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https://www.readbyqxmd.com/read/28748500/digital-droplet-pcr-ddpcr-for-the-detection-and-quantification-of-hpv-16-18-33-and-45%C3%A2-a-short-report
#1
Gabriella Lillsunde Larsson, Gisela Helenius
PURPOSE: Human papilloma virus (HPV) infection is associated with several anogenital malignancies. Here, we set out to evaluate digital droplet PCR (ddPCR) as a tool for HPV 16, 18, 33 and 45 viral load quantification and, in addition, to compare the efficacy of the ddPCR assay for HPV 16 detection with that of quantitative real-time PCR (qPCR). METHODS: Clinical samples, positive for HPV genotypes 16, 18, 33 and 45 were analyzed for viral load using ddPCR. Sample DNA was cleaved before droplet generation and PCR...
July 26, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28748477/chromogenic-in-situ-hybridization-methods-for-microrna-biomarker-monitoring-of-drug-safety-and-efficacy
#2
Barbara R Gould, Tina Damgaard, Boye Schnack Nielsen
Disease research and treatment development have turned to the impact and utility of microRNA. The dynamic and highly specific expression of these molecular regulators can be used to predict and monitor disease progression as well as therapeutic treatment efficacy and safety, thus aiding decisions in patient care. In situ hybridization (ISH) of biopsy material has become a routine clinical pathology procedure for monitoring gene structure, expression, and sample characterization. For ribonucleic acid (RNA), determining cell source and level of expression of these biomarkers gives insight into the cellular function and physiopathology...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28744097/comparison-of-corneal-power-obtained-from-verion-image-guided-surgery-system-and-four-other-devices
#3
Hung-Yuan Lin, Hsin-Yang Chen, Han Bor Fam, Ya-Jung Chuang, Ronald Yeoh, Pi-Jung Lin
PURPOSE: To assess the corneal keratometric values obtained using the VERION image-guided surgery system and other devices. METHODS: This study evaluated the right eyes of 115 cataract patients before intraocular lens (IOL) implantation through consecutive tests using 5 devices: VERION Reference Unit, Placido-based corneal topography (OPD-Scan III), monochromatic light-emitting diodes (LenStar LS900 and AL-Scan), and rotary prism technology (auto kerato-refractometer KR-8800)...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28738642/terminal-protection-mediated-autocatalytic-cascade-amplification-coupled-with-graphene-oxide-fluorescence-switch-for-sensitive-and-rapid-detection-of-folate-receptor
#4
Rui Wang, Xiaowen Xu, Pei Li, Yan Wang, Wei Jiang
Folate receptor (FR) is over-expressed in most human tumors and has been regarded as biomarker and therapeutic target. Specific and sensitive detection of FR is essential for tumor treatment and drug development. Here, a specific, sensitive and rapid FR detection strategy was proposed based on terminal protection-mediated autocatalytic cascade amplification coupled with graphene oxide fluorescence switch. Firstly, the specific binding of FR to the folate terminally-labeled on a primary trigger DNA (PT-DNA) could protect the PT-DNA from exonuclease I degradation, converting FR detection to PT-DNA detection...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738581/the-g-bhq-synergistic-effect-improved-double-quenching-molecular-beacons-based-on-guanine-and-black-hole-quencher-for-sensitive-simultaneous-detection-of-two-dnas
#5
Dongshan Xiang, Fengquan Li, Chenyi Wu, Boan Shi, Kun Zhai
We designed two double quenching molecular beacons (MBs) with simple structure based on guanine (G base) and Black Hole Quencher (BHQ), and developed a new analytical method for sensitive simultaneous detection of two DNAs by synchronous fluorescence analysis. In this analytical method, carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were respectively selected as fluorophore of two MBs, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were respectively selected as organic quencher, and three continuous nucleotides with G base were connected to organic quencher (BHQ-1 and BHQ-2)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28723114/simultaneous-imaging-of-three-tumor-related-mrnas-in-living-cells-with-a-dna-tetrahedron-based-multicolor-nanoprobe
#6
Song Wang, Mengchan Xia, Jie Liu, Sichun Zhang, Xinrong Zhang
We constructed a DNA tetrahedron based multicolor nanoprobe, which could simultaneously imaging of three tumor-related mRNAs in living cells through fluorescence restoration caused by competitive chain replacement reaction. The oligonucleotides used to construct the tetrahedron were extended by adding three 21-base recognition sequences modified with different fluorophores (FAM, Cy3, and Cy5) in the 5' end. Three 11-base complementary sequences modified with quencher (BHQ1 for FAM and BHQ2 for Cy3 and Cy5) were hybridized with the recognition sequences to quench the fluorescence...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28704705/a-new-parasitic-chytrid-staurastromyces-oculus-rhizophydiales-staurastromycetaceae-fam-nov-infecting-the-freshwater-desmid-staurastrum-sp
#7
Silke Van den Wyngaert, Kensuke Seto, Keilor Rojas-Jimenez, Maiko Kagami, Hans-Peter Grossart
Chytrids are a diverse group of ubiquitous true zoosporic fungi. The recent molecular discovery of a large diversity of undescribed chytrids has raised awareness on their important, but so far understudied ecological role in aquatic ecosystems. In the pelagic zone, of both freshwater and marine ecosystems, many chytrid species have been morphologically described as parasites on almost all major groups of phytoplankton. However, the majority of these parasitic chytrids has rarely been isolated and lack DNA sequence data, resulting in a large proportion of "dark taxa" in databases...
May 13, 2017: Protist
https://www.readbyqxmd.com/read/28701205/art-therapy-and-music-reminiscence-activity-in-the-prevention-of-cognitive-decline-study-protocol-for-a-randomized-controlled-trial
#8
Rathi Mahendran, Iris Rawtaer, Johnson Fam, Jonathan Wong, Alan Prem Kumar, Mihir Gandhi, Kenny Xu Jing, Lei Feng, Ee Heok Kua
BACKGROUND: Attention has shifted to the use of non-pharmacological interventions to prevent cognitive decline as a preventive strategy, as well as for those at risk and those with mild cognitive impairment. Early introduction of psycho-social interventions can address cognitive decline and significantly impact quality of life and the wellbeing of elderly individuals. This pilot study explores the feasibility of using art therapy and music reminiscence activity to improve the cognition of community living elderly with mild cognitive impairment...
July 12, 2017: Trials
https://www.readbyqxmd.com/read/28694291/differential-regulation-of-mas-related-g-protein-coupled-receptor-x2-mediated-mast-cell-degranulation-by-antimicrobial-host-defense-peptides-and-p-gingivalis-lps
#9
Kshitij Gupta, Chizobam Idahosa, Saptarshi Roy, Donguk Lee, Hariharan Subramanian, Anuradha Dhingra, Kathleen Boesze-Battaglia, Jonathan Korostoff, Hydar Ali
Porphyromonas gingivalis (P. gingivalis) is a keystone pathogen that contributes to periodontal pathogenesis by disrupting host-microbe homeostasis and promoting dysbiosis. Virulence of P. gingivalis likely reflects an alteration in the lipid A composition of its lipopolysaccharide (LPS) from the penta-acylated (PgLPS1690) to the tetra-acylated (PgLPS1435/1449) form. Mast cells play an important role in periodontitis but the mechanisms of their activation and regulation remain unknown. The expression of epithelium and neutrophil-derived host defense peptides (HDPs; LL-37, human β-defensin-3), which activate mast cells via Mas-related G protein coupled receptor X2 (MRGPRX2), is increased in periodontitis...
July 10, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28691919/modeling-and-analysis-of-a-meso-hydraulic-climbing-robot-with-artificial-muscle-actuation
#10
Edward M Chapman, Tyler E Jenkins, Matthew Bryant
This paper presents a fully coupled electro-hydraulic model of a bio-inspired climbing robot actuated by fluidic artificial muscles (FAMs). This analysis expands upon previous FAM literature by considering not only the force and contraction characteristics of the actuator, but the complete hydraulic and electromechanical circuits as well as the dynamics of the climbing robot. This analysis allows modeling of the time-varying applied pressure, electrical current, and actuator contraction for accurate prediction of the robot motion, energy consumption, and mechanical work output...
July 10, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28690254/mno2-nanosheet-based-fluorescence-sensing-platform-for-sensitive-detection-of-endonuclease
#11
Chao Hu, Xiang Juan Kong, Ru Qin Yu, Ting Ting Chen, Xia Chu
A novel fluorescence sensing platform for ultrasensitive detection of S1 nuclease activity has been constructed based on MnO2 nanosheets and FAM labeled single-stranded DNA (FAM-ssDNA). In this system, MnO2 nanosheets were found to have different adsorbent ability toward ssDNA and mono- or oligonucleotide fragments. FAM-ssDNA could adsorb on MnO2 nanosheets and resulted in significant fluorescence quenching through fluorescence resonance energy transfer (FRET), while mono- or oligonucleotide fragments could not adsorb on MnO2 nanosheets and still retained strong fluorescence emission...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28685314/conformability-in-everolimus-eluting-bioresorbable-scaffolds-compared-with-metal-platform-coronary-stents-in-long-lesions
#12
Jiang Ming Fam, Yuki Ishibashi, Cordula Felix, Bu Chun Zhang, Roberto Diletti, Nicolas van Mieghem, Evelyn Regar, Ron van Domburg, Yoshinobu Onuma, Robert-Jan van Geuns
The aim of this study was to determine if there are significant differences in curvature of the treated vessel after the deployment of a polymeric BRS or MPS in long lesions. The impact of long polymeric bioresorbable scaffolds (BRS) compared with metallic platform stents (MPS) on vessel curvature is unknown. This retrospective study compares 32 patients who received a single everolimus-eluting BRS with 32 patients treated with a single MPS of 28 mm. Quantitative coronary angiography (QCA) was used to evaluate curvature of the treatment and peri-treatment region before and after percutaneous coronary intervention (PCI)...
July 6, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28683562/gaze-to-faces-across-interactive-contexts-in-infants-at-heightened-risk-for-autism
#13
Devon N Gangi, A J Schwichtenberg, Ana-Maria Iosif, Gregory S Young, Fam Baguio, Sally Ozonoff
Infant social-communicative behavior, such as gaze to the face of an interactive partner, is an important early developmental skill. Children with autism spectrum disorder exhibit atypicalities in social-communicative behavior, including gaze and eye contact. Behavioral differences in infancy may serve as early markers of autism spectrum disorder and help identify individuals at highest risk for developing the disorder. Researchers often assess social-communicative behavior in a single interactive context, such as during assessment with an unfamiliar examiner or play with a parent...
July 1, 2017: Autism: the International Journal of Research and Practice
https://www.readbyqxmd.com/read/28658011/the-clinical-alliance-and-research-in-electroconvulsive-therapy-network-an-australian-initiative-for-improving-service-delivery-of-electroconvulsive-therapy
#14
Donel M Martin, Verònica Gálvez, Shani Lauf, Vanessa Dong, Stephen A Baily, Narcís Cardoner, Herng N Chan, Don Davidson, Johnson Fam, Nick De Felice, Erika Martinez-Amoros, Titus Mohan, Jothi Ramalingam, Shanthi I Sarma, Phern C Tor, Susan Waite, Colleen K Loo
OBJECTIVE: There is currently substantial heterogeneity in electroconvulsive therapy (ECT) treatment methods between clinical settings. Understanding how this variation in clinical practice is related to treatment outcomes is essential for optimizing service delivery. The Clinical Alliance and Research in ECT Network is a clinical and research framework with the aims of improving clinical practice, enabling auditing and benchmarking, and facilitating the collection of naturalistic clinical data...
June 28, 2017: Journal of ECT
https://www.readbyqxmd.com/read/28653992/flexible-packaging-by-film-assisted-molding-for-microintegration-of-inertia-sensors
#15
Daniel Hera, Armin Berndt, Thomas Günther, Stephan Schmiel, Christine Harendt, André Zimmermann
Packaging represents an important part in the microintegration of sensors based on microelectromechanical system (MEMS). Besides miniaturization and integration density, functionality and reliability in combination with flexibility in packaging design at moderate costs and consequently high-mix, low-volume production are the main requirements for future solutions in packaging. This study investigates possibilities employing printed circuit board (PCB-)based assemblies to provide high flexibility for circuit designs together with film assisted transfer molding (FAM) to package sensors...
June 27, 2017: Sensors
https://www.readbyqxmd.com/read/28638960/comparative-diagnostic-evaluation-of-omp31-gene-based-taqman%C3%A2-real-time-pcr-assay-with-visual-lamp-assay-and-indirect-elisa-for-caprine-brucellosis
#16
Suman Saini, V K Gupta, K Gururaj, D D Singh, R V S Pawaiya, N K Gangwar, A K Mishra, Deepak Dwivedi, Dimple Andani, Ashok Kumar, T K Goswami
Brucellosis is one of the leading causes of abortion in domestic animals that imposes costs on both economy and society. The disease is highly zoonotic and poses risk to animal handlers due to its zoonotic nature. It causes stillbirth, loss of kids and abortion in last term of pregnancy. Reproductive damage includes infertility in does and orchitis and epididymitis in breeding bucks, which result in high financial losses to farmers and the agriculture industry as a whole. It requires highly sensitive and specific assays to diagnose the disease at field level...
August 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28635171/replacing-paps-in-vitro-phase-ii-sulfation-of-steroids-with-the-liver-s9-fraction-employing-atp-and-sodium-sulfate
#17
Sumudu A Weththasinghe, Christopher C Waller, Han Ling Fam, Bradley J Stevenson, Adam T Cawley, Malcolm D McLeod
In vitro technologies provide the capacity to study drug metabolism where in vivo studies are precluded due to ethical or financial constraints. The metabolites generated by in vitro studies can assist anti-doping laboratories to develop protocols for the detection of novel substances that would otherwise evade routine screening efforts. In addition, professional bodies such as the Association of Official Racing Chemists (AORC) currently permit the use of in vitro derived reference materials for confirmation purposes providing additional impetus for the development of cost effective in vitro metabolism platforms...
June 21, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28631624/printing-induced-cell-injury-evaluation-during-laser-printing-of-3t3-mouse-fibroblasts
#18
Zhengyi Zhang, Wenxuan Chai, Ruitong Xiong, Lei Zhou, Yong Huang
Three-dimensional bioprinting has emerged as a promising solution for the freeform fabrication of living cellular constructs, which can be used for tissue/organ transplantation and tissue models. During bioprinting, some living cells are unavoidably injured and may become necrotic or apoptotic cells. This study aims to investigate the printing-induced cell injury and evaluates injury types of post-printing cells using the annexin V/7-aminoactinomycin D and FAM-DEVD-FMK/propidium iodide assays during laser printing of NIH 3T3 mouse fibroblasts...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28602196/sequence-specific-recognition-of-hiv-1-dsdna-in-the-large-amount-of-normal-dsdna-based-upon-nicking-enzyme-signal-amplification-and-triplex-dna
#19
Houya Zhu, Manjun Zhang, Li Zou, Ruimin Li, Liansheng Ling
A sensitive fluorescent strategy for sequence specific recognition of HIV dsDNA was established based upon Nicking Enzyme Signal Amplification (NESA) and triplex formation. dsDNA sequence from the site 7960 to site 7991 of the HIV1 dsDNA gene was designed as target dsDNA, which was composed of two complementary strands Oligonucleotide 1 with the sequence of 3'-CTT CCT TAT CTT CTT CTT CCA CCT CTC TCT CT-5' (Oligo-1) and Oligonucleotide 2 with the sequence of 5'-GAA GGA ATA GAA GAA GAA GGT GGA GAG AGA GA-3' (Oligo-2)...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28591083/investigating-the-fear-avoidance-model-in-people-with-whiplash-the-association-between-fear-of-movement-and-in-vivo-activity
#20
Ashley Pedler, Steven J Kamper, Annick Maujean, Michele Sterling
OBJECTIVES: The aims of this study were to examine the relationship between fear of movement and activity levels in people with WAD over the first 3 months post injury, to determine the mediating effect of: fear of movement on the relationship between catastrophising and activity levels, and activity levels on the relationship between fear of movement and disability in patients with WAD. METHODS: Ambulatory monitoring of physical activity was conducted for a minimum of 8 waking hours on 2 consecutive days within 4 weeks post injury and at 12 weeks post injury for 103 (74 female) people with WAD...
June 6, 2017: Clinical Journal of Pain
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