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https://www.readbyqxmd.com/read/28930077/bioresorbable-vascular-scaffold-in-chronic-total-occlusions
#1
Jiang Ming Fam, Roberto Diletti
No abstract text is available yet for this article.
September 20, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28919154/is-the-search-for-a-pain-personality-of-added-value-to-the-fear-avoidance-model-fam-of-chronic-pain
#2
Katja Boersma
No abstract text is available yet for this article.
September 11, 2017: Scandinavian Journal of Pain
https://www.readbyqxmd.com/read/28917781/fluorescence-self-quenching-assay-for-the-detection-of-target-collagen-sequences-using-a-short-probe-peptide
#3
Linge Nian, Yue Hu, Caihong Fu, Chen Song, Jie Wang, Jianxi Xiao
The development of novel assays to detect collagen fragments is of utmost importance for diagnostic, prognostic and therapeutic decisions in various collagen-related diseases, and one essential question is to discover probe peptides that can specifically recognize target collagen sequences. Herein we have developed the fluorescence self-quenching assay as a convenient tool to screen the capability of a series of fluorescent probe peptides of variable lengths to bind with target collagen peptides. We have revealed that the targeting ability of probe peptides is length-dependent, and have discovered a relatively short probe peptide FAM-G(POG)8 capable to identify the target peptide...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28916748/%C3%AE-2-adrenoceptor-mediated-inhibition-in-the-central-amygdala-blocks-fear-conditioning
#4
N M Holmes, J W Crane, M Tang, J Fam, R F Westbrook, A J Delaney
The central amygdala is critical for the acquisition and expression of fear memories. This region receives a dense innervation from brainstem noradrenergic cell groups and has a high level of α2-adrenoceptor expression. Using whole-cell electrophysiological recordings from rat brain slices, we characterise the role of pre-synaptic α2-adrenoceptor in modulating discrete inhibitory and excitatory connections within both the lateral and medial division of the central amygdala. The selective α2-adrenoceptor agonist clonidine blocked the excitatory input from the pontine parabrachial neurons onto neurons of the lateral central amygdala...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916108/a-eu-iii-doped-metal-organic-framework-conjugated-with-fluorescein-labeled-single-stranded-dna-for-detection-of-cu-ii-and-sulfide
#5
Han Weng, Bing Yan
In this paper, Bio-MOF-1 is prepared as reported and then Eu(3+) is introduced into it via cation exchange method. A FAM-labeled ssDNA is chosen to fabricate with the obtained Eu(3+)@Bio-MOF-1. A luminescent hybrid material is assembled, which can exhibit the fluorescence of Eu(3+) and FAM simultaneously by adjusting the ratio of FAM-ssDNA and Eu(3+)@Bio-MOF-1. The sample is then used for the detecting of metal ions, results shows which has good selectively for Cu(2+) (LOD = 0.14 μM, 0-250 μM). The introduction of Cu(2+) can quench the fluorescence of FAM while the luminescent intensity of Eu(3+) enhancing...
October 2, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28905708/williamwhitmania-taraxaci-gen-nov-sp-nov-a-proteolytic-anaerobe-with-a-novel-type-of-cytology-from-lake-untersee-in-antarctica-description-of-williamwhitmaniaceae-fam-nov-and-emendation-of-the-order-bacteroidales-krieg-2012
#6
Elena V Pikuta, Zhe Lyu, Richard B Hoover, Yuchen Liu, Nisha B Patel, Hans J Busse, Paul A Lawson
The proteolytic bacterium strain A7P-90mT was isolated from Lake Untersee, Antarctica. The anoxic water was collected from a perennially sealed (~100 millennia) glacial ice lake. Gram-stain-negative cells were 0.18-0.3×8.0-25.0 µm in size, straight, slender rods with unusual gliding motility by external, not previously reported, organelles named here as antiae. At the end of stationary phase of growth, spheroplasts were terminally formed and the cells resembled dandelions. After death, cells were helical...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28898132/staged-residential-post-acute-rehabilitation-for-adults-following-acquired-brain-injury-a-comparison-of-functional-gains-rated-on-the-uk-functional-assessment-measure-uk-fim-fam-and-the-mayo-portland-adaptability-inventory-mpai-4
#7
Diana Jackson, Karla Seaman, Kristylee Sharp, Rachel Singer, Janet Wagland, Lynne Turner-Stokes
OBJECTIVE: To compare the UK Functional Assessment Measure (UK FIM+FAM) and Mayo-Portland Adaptability Inventory (MPAI-4) as measures of functional change in patients with brain injury receiving a staged residential post-acute community-based rehabilitation programme. RESEARCH DESIGN: Longitudinal cohort study of consecutive admissions (N = 42) over 3 years. METHODS: Patients were assessed at admission and discharge/annual review. We examined groups according to stage of independence on admission: Maximum support (stages 1 and 2: N = 17); moderate/maximum self-care/household support (stage 3: N = 15); minimal self-care and moderate household/community support (stages 4-6: N = 10)...
September 12, 2017: Brain Injury: [BI]
https://www.readbyqxmd.com/read/28889204/a-microfluidics-based-mobility-shift-assay-to-identify-new-inhibitors-of-%C3%AE-secretase-for-alzheimer-s-disease
#8
Rongfeng Liu, Yu-Chih Liu, Junwei Meng, Haiyan Zhu, Xuehong Zhang
The β-secretase (BACE1) initiates the generation of toxic amyloid-β peptide (Aβ) from amyloid-β precursor protein (APP), which was widely considered to play a key role in the pathogenesis of Alzheimer's disease (AD). Here, a novel microfluidics-based mobility shift assay (MMSA) was developed, validated, and applied for the screening of BACE1 inhibitors for AD. First, the BACE1 activity assay was established with a new fluorescent peptide substrate (FAM-EVNLDAEF) derived from the Swedish mutant APP, and high-quality ratiometric data were generated in both endpoint and kinetic modes by electrophoretic separation of peptide substrate from the BACE1 cleaved product (FAM-EVNL) before fluorescence quantification...
September 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28887671/histamine-2-receptor-antagonism-elicits-protection-against-doxorubicin-induced-cardiotoxicity-in-rodent-model
#9
Sundar Kumar Kondru, Ajay Godwin Potnuri, Lingesh Allakonda, Prasad Konduri
Doxorubicin (DOX), an anthracycline-based antibiotic, is regularly used in the management of carcinomas, and haematological malignancies have been downplayed in chemotherapy because of its ability to induce dilated cardiomyopathy (DCM). Dexrazoxane is approved to combat the cardiotoxicity, but limited by its adverse effects. Redox imbalance and reactive oxygen species generation plays major role in DOX-induced cardiotoxicity. Histamine, known to mediate various cardiovascular effects, but nevertheless the role of histamine or its receptors in DOX-induced DCM is remained obscure...
September 8, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28887375/csf-inflammatory-response-after-intraventricular-hemorrhage
#10
Maged D Fam, Hussein A Zeineddine, Javed Khader Eliyas, Agnieszka Stadnik, Michael Jesselson, Nichol McBee, Karen Lane, Ying Cao, Meijing Wu, Lingjiao Zhang, Richard E Thompson, Sayona John, Wendy Ziai, Daniel F Hanley, Issam A Awad
OBJECTIVE: To investigate the temporal pattern and relevant associations of CSF inflammatory measures after intraventricular hemorrhage (IVH). METHODS: We analyzed prospectively collected CSF cell counts and protein and glucose levels from participants in the Clot Lysis Evaluation of Accelerated Resolution of IVH phase III (CLEAR III) trial. Corrected leukocyte count and cell index were calculated to adjust for CSF leukocytes attributable to circulating blood. Data were chronologically plotted...
September 8, 2017: Neurology
https://www.readbyqxmd.com/read/28884673/reclassification-of-halothiobacillus-hydrothermalis-and-halothiobacillus-halophilus-to-guyparkeria-gen-nov-in-the-thioalkalibacteraceae-fam-nov-with-emended-descriptions-of-the-genus-halothiobacillus-and-family-halothiobacillaceae
#11
Rich Boden
The genus Halothiobacillus contains four species of obligate autotrophs with validly published names, of which Halothiobacillus halophilus and Halothiobacillus hydrothermalis are very distant from the type species - on the basis of the 16S rRNA gene, they have 90.7 % and 90.9 % identity to that of the type species, Halothiobacillus neapolitanus. As these values fall below the Yarza cut-off for the rank of genus, and these two species also show no clear affiliation to the closely related genus Thioalkalibacter, a polyphasic study was undertaken to determine if they represent a separate genus...
September 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28878894/genomic-regions-and-pathways-associated-with-gastrointestinal-parasites-resistance-in-santa-in%C3%A3%C2%AAs-breed-adapted-to-tropical-climate
#12
Mariana Piatto Berton, Rafael Medeiros de Oliveira Silva, Elisa Peripolli, Nedenia Bonvino Stafuzza, Jesús Fernández Martin, Maria Saura Álvarez, Beatriz Villanueva Gavinã, Miguel Angel Toro, Georgget Banchero, Priscila Silva Oliveira, Joanir Pereira Eler, Fernando Baldi, José Bento Sterman Ferraz
BACKGROUND: The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with resistance to gastrointestinal parasites, particularly Haemonchus contortus, in a breed of sheep adapted to tropical climate. Phenotypes evaluations were performed to verify resistance to gastrointestinal parasites, and were divided into two categories: i) farm phenotypes, assessing body condition score (BCS), degree of anemia assessed by the famacha chart (FAM), fur score (FS) and feces consistency (FC); and ii) lab phenotypes, comprising blood analyses for hematocrit (HCT), white blood cell count (WBC), red blood cell count (RBC), hemoglobin (HGB), platelets (PLT) and transformed (log10) egg per gram of feces (EPGlog)...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28877431/in-situ-imaging-of-tissue-remodeling-with-collagen-hybridizing-peptides
#13
Jeongmin Hwang, Yufeng Huang, Timothy J Burwell, Norman C Peterson, Jane Connor, Stephen J Weiss, S Michael Yu, Yang Li
Collagen, the major structural component of nearly all mammalian tissues, undergoes extensive proteolytic remodeling during developmental states and a variety of life-threatening diseases such as cancer, myocardial infarction, and fibrosis. While degraded collagen could be an important marker of tissue damage, it is difficult to detect and target using conventional tools. Here, we show that a designed peptide (collagen hybridizing peptide: CHP), which specifically hybridizes to the degraded, unfolded collagen chains, can be used to image degraded collagen and inform tissue remodeling activity in various tissues: labeled with 5-carboxyfluorescein and biotin, CHPs enabled direct localization and quantification of collagen degradation in isolated tissues within pathologic states ranging from osteoarthritis and myocardial infarction to glomerulonephritis and pulmonary fibrosis, as well as in normal tissues during developmental programs associated with embryonic bone formation and skin aging...
September 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28875900/sulfurivermis-fontis-gen-nov-sp-nov-a-sulfur-oxidizing-autotroph-and-proposal-of-thioprofundaceae-fam-nov
#14
Hisaya Kojima, Miho Watanabe, Manabu Fukui
A novel Gram-stain-negative, chemolithoautotrophic sulfur oxidizer, strain JG42T, was isolated from a hot spring microbial mat. As an electron donor for autotrophic growth, strain JG42T utilized sulfide, thiosulfate, tetrathionate and elemental sulfur. Cells of strain JG42T were oxidase-positive and catalase-negative. The G+C content of the genomic DNA was 65 mol%. The predominant cellular fatty acid was C16 : 0. Phylogenetic analysis of the 16S rRNA gene indicated that strain JG42T belonged to the order Chromatiales, but sequence similarities to the known species were less than 94 %...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28870881/transcatheter-edge-to-edge-repair-of-lead-associated-tricuspid-regurgitation
#15
Neil P Fam, Edwin C Ho, Naveed Ahmed, Kim A Connelly
No abstract text is available yet for this article.
September 5, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28853681/proposal-for-the-reclassification-of-obligately-purine-fermenting-bacteria-clostridium-acidurici-barker-1938-and-clostridium-purinilyticum-d%C3%A3-rre-et-al-1981-as-gottschalkia-acidurici-gen-nov-comb-nov-and-gottschalkiapurinilytica-comb-nov-and-of-eubacterium
#16
Anja Poehlein, Natalya Yutin, Rolf Daniel, Michael Y Galperin
Several strictly anaerobic bacteria that are Gram-stain-positive have the ability to use uric acid as the sole source of carbon and energy. The phylogeny of three such species, Clostridium acidurici, Clostridium purinilyticum, and Eubacterium angustum, members of the Clostridium cluster XII that ferment purines, but not most amino acids or carbohydrates, has been re-examined, taking advantage of their recently sequenced genomes. Phylogenetic analyses, based on 16S rRNA gene sequences, protein sequences of RpoB and GyrB, and on a concatenated alignment of 50 ribosomal proteins, revealed tight clustering of C...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28853135/epidemiological-characteristics-and-trends-in-the-incidence-of-animal-bites-in-maku-county-islamic-republic-of-iran-2003-2012
#17
Seyed Morteza Shamshirgaran, Hamid Barzkar, Saber Ghaffari-Fam, Ahmad Kosha, Parvin Sarbakhsh, Pari Ghasemzadeh
This study examined the epidemiological characteristics and incidence over time of animal bites in Maku County. Data were obtained from health centre records of animal bites from 2003 to 2012. Information on demographic characteristics and bite-related factors were recorded. A total of 2232 people were bitten; the frequency of bites was highest among males (75.4%), rural residents (72.3%) and those < 20 years (47.3%). Most bites were to the legs and feet (65%) and dogs were responsible for 92% of bites. Over 25% of those bitten delayed seeking care for more than a day, particularly rural residents...
August 27, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/28841253/identification-of-highly-expressed-plasmodium-vivax-proteins-from-clinical-isolates-using-proteomics
#18
Apoorva Venkatesh, Anwesha Lahiri, Panga Jaipal Reddy, Jayanthi Shastri, Sheetal Bankar, Swati Patankar, Sanjeeva Srivastava
PURPOSE: Plasmodium vivax is the most geographically widespread species responsible for malaria in humans. Our study focused on identifying highly expressed parasite proteins using a shotgun proteomics approach. EXPERIMENTAL DESIGN: Parasites (P. vivax) were isolated from 7 patient samples using saponin lysis. Protein extracts from these parasites were processed and subjected to LC-MS/MS analysis. RESULTS: An overall proteome coverage of 586 P...
August 25, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28840802/mabikibacter-ruber-gen-nov-sp-nov-a-bacterium-isolated-from-marine-sediment-and-proposal-of-mabikibacteraceae-fam-nov-in-the-class-alphaproteobacteria
#19
Grace Choi, Ming Gao, Dae-Sung Lee, Hyukjae Choi
Bacterial strain YP382-1-AT was isolated from marine sediment at Gimnyeong Beach, Jeju Island, Republic of Korea. This strain was found to be aerobic and Gram-stain-negative, with red to purple pigmentation. Cells of strain YP382-1-AT were motile, had a single polar flagellum and were short rod-shaped. The predominant quinone in strain YP382-1-AT was Q-10. The major fatty acid was C18 : 1ω7c and the DNA G+C content was 60.3 mol%. Phylogenetic analysis based on comparisons of 16S rRNA gene sequences showed that strain YP382-1-AT belonged to the order Rhizobiales in the class Alphaproteobacteria...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28838478/2-year-outcomes-after-transcatheter%C3%A2-mitral-valve-replacement
#20
Ander Regueiro, Jian Ye, Neil Fam, Vinayak N Bapat, François Dagenais, Mark D Peterson, Stephan Windecker, John G Webb, Josep Rodés-Cabau
OBJECTIVES: This study sought to determine late (2-year) outcomes following transcatheter mitral valve replacement (TMVR) with the FORTIS valve (Edwards Lifesciences, Irvine, California). BACKGROUND: No data exist on long-term clinical outcomes following TMVR in patients with severe native mitral regurgitation (MR). METHODS: This multicenter registry included consecutive patients with severe MR who underwent TMVR with the FORTIS valve under a compassionate clinical use program...
August 28, 2017: JACC. Cardiovascular Interventions
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