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Edwin C Ho, Neil P Fam
Advancements in transcatheter technology has increased the number of therapeutic options for patients with tricuspid regurgitation. One category of repair devices attempts to augment leaflet coaptation by either leaflet plication or by functioning as a space occupying device. These include the MitraClip (Abbott, Santa Clara, CA, USA), PASCAL (Edwards Lifesciences, Irvine, CA, USA) and FORMA (Edwards Lifesciences, Irvine, CA, USA) devices. All three have been successfully implanted with promising short term echocardiographic and clinical outcomes...
March 15, 2018: Minerva Cardioangiologica
Yumao Jiang, Yue Jiao, Yang Liu, Meiyu Zhang, Zhiguo Wang, Yujuan Li, Tao Li, Xiaoliang Zhao, Danqiao Wang
As shown in our previous study, sinomenine hydrochloride (SH), the major bioactive alkaloid isolated from Sinomenium acutum Rehd. et Wils. (Fam. Menispermaceae ), initiates the autophagy-mediated death of human glioblastoma cells by generating reactive oxygen species and activating the autophagy-lysosome pathway. However, its effects on the migration and invasion of human glioblastoma cells have not yet been elucidated. Therefore, human glioblastoma U87 and SF767 cells were treated with SH (0.125 and 0.25 mM) for 24 h, and cell migration and invasion were assessed using scratch wound healing, migration and invasion assays...
March 14, 2018: International Journal of Molecular Sciences
Jianbang Wang, Zhixin Zhou, Liang Yue, Shan Wang, Itamar Willner
The switchable reconfiguration of a mixture of two dimers of DNA origami tiles AB and CD into a mixture of two DNA origami dimers composed of AD and CB, using collection of fuel and anti-fuel strands, is presented. The reversible reconfiguration of the mixture of dimers AB/CD into AD/CB followed by labeling each of the tile with 0, 1, 2 and 3 4× hairpins labels and by imaging the dimer structures by AFM. Subjecting the reconfigurable dimer mixtures to a collection of Mg2+ -dependent DNAzyme subunits and the substrates consisting of the ROX/BHQ2-modified substrate and the FAM/BHQ1-modified substrate leads to the triggered and programmed switchable operation in the presence of appropriate fuel and anti-fuel strands...
March 14, 2018: Nano Letters
Jianhao Wang, Zhilan Zhu, Xiang Wang, Li Yang, Li Liu, Jianpeng Wang, Eseosaserea Igbinigie, Xiaoqian Liu, Jinping Li, Lin Qiu, Yong-Qiang Li, Pengju Jiang
Recently, the in-capillary electrophoresis assay has been applied to variety kinds of analyses owing to its multiple functional integrating features, including mixing of samples, reaction process of the mixtures, and the separation and detection in one capillary system. However, the micro-reactor still has its limitations to the currently available applications, especially the mixing step of the samples inside the capillary could not be well controlled automatically or manually. Herein, we have developed a novel capillary electrophoresis assay for the detection of antibody-peptide binding inside a bending capillary...
March 7, 2018: International Journal of Biological Macromolecules
Hok Khim Fam, Kunho Choi, Lauren Fougner, Chinten James Lim, Cornelius F Boerkoel
Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a nuclear and mitochondrial protein that in nuclei and in vitro repairs blocked 3' DNA termini such as 3' phosphotyrosine conjugates resulting from stalling of topoisomerase I-DNA intermediates. Its mutation also causes spinocerebellar ataxia with axonal neuropathy type 1 (SCAN1). Because Tdp1 colocalizes with mitochondria following oxidative stress, we hypothesized that Tdp1 repairs mitochondrial DNA (mtDNA) and that mtDNA damage mediates entry of Tdp1 into the mitochondria...
March 9, 2018: Scientific Reports
Tarek Zghaib, Ali Keramati, Jonathan Chrispin, Dong Huang, Muhammad A Balouch, Luisa Ciuffo, Ronald D Berger, Joseph E Marine, Hiroshi Ashikaga, Hugh Calkins, Saman Nazarian, David D Spragg
Background: Bipolar voltage mapping, as part of atrial fibrillation (AF) ablation, is traditionally performed in a point-by-point (PBP) approach using single-tip ablation catheters. Alternative techniques for fibrosis-delineation include fast-anatomical mapping (FAM) with multi-electrode circular catheters, and late gadolinium-enhanced magnetic-resonance imaging (LGE-MRI). The correlation between PBP, FAM, and LGE-MRI fibrosis assessment is unknown. Objective: In this study, we examined AF substrate using different modalities (PBP, FAM, and LGE-MRI mapping) in patients presenting for an AF ablation...
January 2018: JACC. Clinical Electrophysiology
Juan Song, Fei Yin, Xia Li, Na Dong, Yingjie Zhu, Yanan Shao, Baoli Chen, Wei Jiang, Chen-Zhong Li
We developed a novel approach to determine formamidopyrimidine DNA glycosylase (FPG) activity by taking advantage of target-induced self-primed rolling circle amplification (RCA) and magnetic nanoprobes. Herein, a unique nick (8-oxoguanine, 8-oxoG) was positioned in duplex DNA containing P-circle and P1 , which together serve as a FPG substrate, RCA template, and RCA primer probe. The presence of FPG specifically binds 8-oxoG and cleaves the P-circle into two parts, producing 5'-phosphoryl termini. A phosphodiester bond between the 5'-phosphoryl and 3'-hydroxyl termini was formed with the addition of T4 DNA ligase, producing an unnicked circular strand...
March 8, 2018: Analyst
Deniz Ortaç, Mustafa Cemek, Turan Karaca, Mehmet E Büyükokuroğlu, Zafer Ö Özdemir, Ayşe Tuba Kocaman, Sadık Göneş
CONTEXT: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis. OBJECTIVE: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute gastric mucosal injury in animal models. MATERIALS AND METHODS: Wistar rats were treated with 500, 250 or 100 mg/kg okra; 20 mg/kg famotidine (Fam); and 75 mg/kg quercetin (Que)...
December 2018: Pharmaceutical Biology
Elizabeth Fam, Jeanne M Ferrante
To help understand and mitigate health disparities, it is important to conduct research with underserved and underrepresented minority populations under real world settings. There is a gap in the literature detailing real-time research staff experience, particularly in their own words, while conducting in-person patient recruitment in urban community health centers. This paper describes challenges faced at the clinic, staff, and patient levels, our lessons learned, and strategies implemented by research staff while recruiting predominantly low-income African-American women for an interviewer-administered survey study in four urban Federally Qualified Health Centers in New Jersey...
February 2018: Journal of the National Medical Association
Faris A Alodaibi, Julie M Fritz, Anne Thackeray, Shane L Koppenhaver, Jeffrey J Hebert
OBJECTIVE: To examine the prognostic value of the Fear Avoidance Model (FAM) variables when predicting pain intensity and disability 10-weeks postoperative following lumbar disc surgery. METHODS: We recruited patients scheduled for first-time, single level lumbar disc surgery. The following aspects of the FAM were assessed at preoperative baseline and after 10 postoperative weeks: numeric pain rating scale (0-10) for leg and back pain intensity separately, Pain Catastrophizing Scale (PCS), Fear Avoidance Beliefs Questionnaire (FABQ), Beck Depression Inventory (BDI), Oswestry Disability Questionnaire (ODI), and the International Physical Activity Questionnaire (IPAQ)...
2018: PloS One
P W Crous, M J Wingfield, T I Burgess, A J Carnegie, G E St J Hardy, D Smith, B A Summerell, J F Cano-Lira, J Guarro, J Houbraken, L Lombard, M P Martín, M Sandoval-Denis, A V Alexandrova, C W Barnes, I G Baseia, J D P Bezerra, V Guarnaccia, T W May, M Hernández-Restrepo, A M Stchigel, A N Miller, M E Ordoñez, V P Abreu, T Accioly, C Agnello, A Agustin Colmán, C C Albuquerque, D S Alfredo, P Alvarado, G R Araújo-Magalhães, S Arauzo, T Atkinson, A Barili, R W Barreto, J L Bezerra, T S Cabral, F Camello Rodríguez, R H S F Cruz, P P Daniëls, B D B da Silva, D A C de Almeida, A A de Carvalho Júnior, C A Decock, L Delgat, S Denman, R A Dimitrov, J Edwards, A G Fedosova, R J Ferreira, A L Firmino, J A Flores, D García, J Gené, A Giraldo, J S Góis, A A M Gomes, C M Gonçalves, D E Gouliamova, M Groenewald, B V Guéorguiev, M Guevara-Suarez, L F P Gusmão, K Hosaka, V Hubka, S M Huhndorf, M Jadan, Ž Jurjević, B Kraak, V Kučera, T K A Kumar, I Kušan, S R Lacerda, S Lamlertthon, W S Lisboa, M Loizides, J J Luangsa-Ard, P Lysková, W P Mac Cormack, D M Macedo, A R Machado, E F Malysheva, P Marinho, N Matočec, M Meijer, A Mešić, S Mongkolsamrit, K A Moreira, O V Morozova, K U Nair, N Nakamura, W Noisripoom, I Olariaga, R J V Oliveira, L M Paiva, P Pawar, O L Pereira, S W Peterson, M Prieto, E Rodríguez-Andrade, C Rojo De Blas, M Roy, E S Santos, R Sharma, G A Silva, C M Souza-Motta, Y Takeuchi-Kaneko, C Tanaka, A Thakur, M Th Smith, Z Tkalčec, N Valenzuela-Lopez, P van der Kleij, A Verbeken, M G Viana, X W Wang, J Z Groenewald
Novel species of fungi described in this study include those from various countries as follows: Antarctica : Cadophora antarctica from soil. Australia : Alfaria dandenongensis on Cyperaceae , Amphosoma persooniae on Persoonia sp., Anungitea nullicana on Eucalyptus sp . , Bagadiella eucalypti on Eucalyptus globulus , Castanediella eucalyptigena on Eucalyptus sp., Cercospora dianellicola on Dianella sp., Cladoriella kinglakensis on Eucalyptus regnans , Cladoriella xanthorrhoeae (incl. Cladoriellaceae fam. nov...
December 2017: Persoonia
A Hashimoto, M Matsumura, K Hirayama, K Tanaka
The family Lophiotremataceae ( Pleosporales , Dothideomycetes ) is taxonomically revised on the basis of morphological observations and phylogenetic analyses of sequences of nuclear rDNA SSU, ITS, and LSU regions and tef1 and rpb2 genes. A total of 208 sequences were generated from species of Lophiotremataceae and its relatives. According to phylogenetic analyses, Lophiotremataceae encompasses the genus Lophiotrema and five new genera: Atrocalyx , Crassimassarina , Cryptoclypeus , Galeaticarpa , and Pseudocryptoclypeus ...
December 2017: Persoonia
Taeeung Kim, Xianghao Ren, Kyu-Jung Chae
This study evaluated the unique features of a filamentous algae matrix (FAM) that can be applied to high rate algal ponds (HRAPs) as a promising way to remove nutrient from polluted rural streams. The results show that the HRAPs, coupled with the FAM, effectively removed nitrogen and phosphorus (79.8% and 81.2%, respectively), and achieved high production of DO, with a maximum of 11.0 g O2 g FAM-1 d-1 . The FAM functioned wells as a screen to prevent excessive algae loss from the system and obtained relatively high biomass growth rate (0...
March 1, 2018: Journal of Environmental Management
Rachel R S Fam, Kum C Hiong, Celine Y L Choo, Wai P Wong, Shit F Chew, Yuen K Ip
Giant clams harbor symbiotic zooxanthellae (Symbiodinium), which are nitrogen-deficient, mainly in the fleshy and colorful outer mantle. This study aimed to sequence and characterize the algal Glutamine Synthetase (GS) and Glutamate Synthase (GLT), which constitute the glutamate synthase cycle (or GS-GOGAT cycle, whereby GOGAT is the protein acronym of GLT) of nitrogen assimilation, from the outer mantle of the fluted giant clam, Tridacna squamosa. We had identified a novel GS-like cDNA coding sequence of 2325 bp, and named it as T...
February 26, 2018: Gene
L Franco-Trigo, J Tudball, D Fam, S I Benrimoj, D Sabater-Hernández
BACKGROUND: Collaboration between relevant stakeholders in health service planning enables service contextualization and facilitates its success and integration into practice. Although community pharmacy services (CPSs) aim to improve patients' health and quality of life, their integration in primary care is far from ideal. Key stakeholders for the development of a CPS intended at preventing cardiovascular disease were identified in a previous stakeholder analysis. Engaging these stakeholders to create a shared vision is the subsequent step to focus planning directions and lay sound foundations for future work...
February 21, 2018: Research in Social & Administrative Pharmacy: RSAP
Oleg N Medvedev, Lynne Turner-Stokes, Stephen Ashford, Richard J Siegert
OBJECTIVES: To determine whether the UK Functional Assessment Measure (UK FIM+FAM) fits the Rasch model in stroke patients with complex disability and, if so, to derive a conversion table of Rasch-transformed interval level scores. METHODS: The sample included a UK multicentre cohort of 1,318 patients admitted for specialist rehabilitation following a stroke. Rasch analysis was conducted for the 30-item scale including 3 domains of items measuring physical, communication and psychosocial functions...
February 28, 2018: Journal of Rehabilitation Medicine
Judith S Kempfle, Kim Nguyen, Christine Hamadani, Nicholas Koen, Albert S Edge, Boris A Kashemirov, David H Jung, Charles E McKenna
Hearing loss affects more than two-thirds of the elderly population, and more than 17% of all adults in the U.S. Sensorineural hearing loss related to noise exposure or aging is associated with loss of inner ear sensory hair cells (HCs), cochlear spiral ganglion neurons (SGNs), and ribbon synapses between HCs and SGNs, stimulating intense interest in therapies to regenerate synaptic function. 7,8-Dihydroxyflavone (DHF) is a selective and potent agonist of tropomyosin receptor kinase B (TrkB) and protects the neuron from apoptosis...
February 27, 2018: Bioconjugate Chemistry
Brian Kim, Kang Wook Chung, Ji Hoon Lee
A cost-effective and simple biosensor was developed for the accurate and rapid monitoring of norovirus GII in a sample. The modified DNA aptamer, 5 guanines linked to conventional DNA aptamer, designed for the research, was able to rapidly capture norovirus GII in tap water as well artificial urine. In addition, the extra guanines of the modified DNA aptamer enhanced the sensitivity of biosensor with guanine chemiluminescence detection based on the principle of intra chemiluminescent resonance transfer (Intra-CRET)...
February 11, 2018: Journal of Pharmaceutical and Biomedical Analysis
Yi-Jun Shi, Liang-Jun Wang, Yuan-Chin Lee, Chia-Hui Huang, Wan-Ping Hu, Long-Sen Chang
This study presents two sensitive fluorescent assays for sensing heparin on the basis of the electrostatic interaction between heparin and Naja naja atra cardiotoxin 3 (CTX3). Owing to CTX3-induced folded structure of an adenosine-based molecular beacon (MB) or a DNA aptamer against CTX3, a reduction in the fluorescent signal of the aptamer or MB 5'-end labeled with carboxyfluorescein (FAM) and 3'-end labeled with 4-([4-(dimethylamino)phenyl]azo)-benzoic acid (DABCYL) was observed upon the addition of CTX3...
February 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Luciana Albuquerque, Ana Rita M Polónia, Cristina Barroso, Hugo J C Froufe, Olga Lage, Alexandre Lobo-da-Cunha, Conceição Egas, Milton S da Costa
An isolate, designated SPSPC-11T , with an optimum growth temperature of about 50 °C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive...
January 10, 2018: International Journal of Systematic and Evolutionary Microbiology
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