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https://www.readbyqxmd.com/read/28530564/a-new-method-for-the-rapid-detection-of-atlantic-cod-gadus-morhua-pacific-cod-gadus-macrocephalus-alaska-pollock-gadus-chalcogrammus-and-ling-molva-molva-using-a-lateral-flow-dipstick-assay
#1
Ledicia Taboada, Ana Sánchez, Ricardo I Pérez-Martín, Carmen G Sotelo
Species-specific lateral flow dipstick (LFD) assays for the identification of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) in food products were developed. The method comprises a PCR system with four sets of specific primers, for each target species. This step was also devised to dual-labeling of PCR products with biotin and 6-FAM, which are then easily read on a lateral flow dipstick, upon which these products are immobilized by a fixed biotin-ligand and visualized with anti-FAM antibody-coated gold nanoparticles...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28512068/potentially-increased-incidence-of-scaffold-thrombosis-in-patients-treated-with-absorb-bvs-who-terminated-dapt-before-18-months
#2
Cordula M Felix, Georgios J Vlachojannis, Alexander J J IJsselmuiden, Jiang M Fam, Peter C Smits, Wouter J Lansink, Roberto Diletti, Felix Zijlstra, Evelyn S Regar, Eric Boersma, Yoshinobu Onuma, Robert J M van Geuns
AIMS: The aim of this study was to investigate the impact of dual antiplatelet therapy (DAPT) termination on late and very late scaffold thrombosis (ScT) in patients treated with the Absorb bioresorbable vascular scaffold (BVS). METHODS AND RESULTS: Data from the registries of three centres were pooled (808 patients). To investigate the effect of DAPT termination on ScT after a minimum of six months, we selected a subgroup ("DAPT study cohort" with 685 patients) with known DAPT status >6 months and excluded the use of oral anticoagulants and early ScT...
May 18, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28502003/microsatellite-analysis-for-identification-of-individuals-using-bone-from-the-extinct-steller-s-sea-cow-hydrodamalis-gigas
#3
Jeffery F Warner, Michael G Harpole, Lorelei D Crerar
Microsatellite DNA can provide more detailed population genetic information than mitochondrial DNA which is normally used to research ancient bone. The methods detailed in this chapter can be utilized for any type of bone. However, for this example, four microsatellite loci were isolated from Steller's sea cow (Hydrodamalis gigas) using published primers for manatee and dugong microsatellites. The primers DduC05 (Broderick et al., Mol Ecol Notes 6:1275-1277, 2007), Tmakb60, TmaSC5 (Pause et al., Mol Ecol Notes 6: 1073-1076, 2007), and TmaE11 (Garcia-Rodriguez et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28499131/polyphyly-in-the-thecate-amoeba-genus-lecythium-chlamydophryidae-tectofilosida-cercozoa-redescription-of-its-type-species-l-hyalinum-description-of-l-jennyae-sp-nov-and-the-establishment-of-fisculla-gen-nov-and-fiscullidae-fam-nov
#4
Kenneth Dumack, Paul Mausbach, Mona Hegmann, Michael Bonkowski
Although testate amoebae have attracted great interest of protistologists for more than a century, some groups, especially those with a hyaline, organic test (=theca) are still poorly known. One of those is the genus Lecythium Hertwig et Lesser, 1874. Only recently Lecythium spp. were characterized by morphological and molecular means, but data on the type species Lecythium hyalinum Hertwig et Lesser, 1874, was still lacking. In this study, we screened for L. hyalinum in freshwater samples of Germany and the Netherlands...
March 14, 2017: Protist
https://www.readbyqxmd.com/read/28498859/usp9x-counteracts-differential-ubiquitination-of-nphp5-by-march7-and-bbs11-to-regulate-ciliogenesis
#5
Arindam Das, Jin Qian, William Y Tsang
Ciliogenesis is a fundamental biological process central to human health. Precisely how this process is coordinated with the cell cycle remains an open question. We report that nephrocystin-5 (NPHP5/IQCB1), a positive regulator of ciliogenesis, is a stable and low turnover protein subjected to cycles of ubiquitination and deubiquitination. NPHP5 directly binds to a deubiquitinating enzyme USP9X/FAM and two E3 ubiquitin ligases BBS11/TRIM32 and MARCH7/axotrophin. NPHP5 undergoes K63 ubiquitination in a cell cycle dependent manner and K48/K63 ubiquitination upon USP9X depletion or inhibition...
May 12, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28495579/a-new-chemotherapy-agent-free-theranostic-system-composed-of-graphene-oxide-nano-complex-and-aptamers-for-treatment-of-cancer-cells
#6
Amirhossein Bahreyni, Rezvan Yazdian-Robati, Shirin Hashemitabar, Mohammad Ramezani, Pouria Ramezani, Khalil Abnous, Seyed Mohammad Taghdisi
The common cancer treatment strategies like chemotherapy and radiotherapy are nonspecific and can trigger severe side effects by damaging normal cells. So, targeted cancer therapies, such as apoptosis induction, have attracted great attention in recent years. In this project, two nano-complexes, MUC1 aptamer-NAS-24 aptamer-Graphene oxide (GO) and MUC1 aptamer-Cytochrome C aptamer-GO, were designed to induce cell programmed death in MDA-MB-231 and MCF-7 cells (breast cancer cell lines) and to verify the level of apoptosis in both cell lines...
May 8, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28486578/development-of-a-validated-comparative-stability-indicating-assay-method-for-some-h2-receptor-antagonists
#7
Sameh Ahmed, Salwa R Elshaboury, Niveen A Mohamed, Shereen Farrag
A comparative force degradation high performance thin layer chromatography (HPTLC) method was developed and validated for some H2-receptor antagonists. The studied H2-receptor antagonists were ranitidine (RAN), nizatidine (NIZ) and famotidine (FAM). The degradation behaviors of the studied H2-receptor antagonists were studied under different stress conditions (hydrolytic, thermal and oxidative) conditions as well as storage conditions according to International Conference on Harmonization (ICH) recommendations...
May 9, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28485277/first-transjugular-edge-to-edge-mitral-valve-repair-with-the-mitraclip-system
#8
Neil P Fam, Edwin C Ho, Naveed Ahmed, Jeremy Edwards
No abstract text is available yet for this article.
May 9, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28481560/an-examination-of-changes-in-behavioral-control-when-stimuli-with-different-associative-histories-are-conditioned-in-compound
#9
Justine Fam, R Frederick Westbrook, Nathan M Holmes
This series of experiments used rats to examine changes in behavioral control when stimuli with different associative histories are conditioned in compound. The initial experiments used blocking designs. Experiment 1 provided a within-subject demonstration of blocking, and Experiment 2 used the compound test procedure to show that, when a novel stimulus, X, is conditioned in compound with an already conditioned stimulus (CS), A, these audiovisual compound stimulus (AX)+ conditioning trials produce a greater increase in behavioral control for X than A...
May 8, 2017: Journal of Experimental Psychology. Animal Learning and Cognition
https://www.readbyqxmd.com/read/28479778/paratesticular-aggressive-angiomyxoma-a-rare-case
#10
Muhamad Izwan Ismail, Yin Ping Wong, Guan Hee Tan, Xeng Inn Fam
Aggressive angiomyxoma (AAM) particularly testicular origin is a rare benign mesenchymal myxoid tumor which is locally aggressive, blatant for local recurrence, and may metastasize. It occurs mostly in females of childbearing age and extremely rare in males. AMM particular testicular origin is not reported in literature yet. This is a 65-year-old man who had a right scrotal swelling. Ultrasound scrotum showed a soft tissue tumor of the right testis. The patient underwent radical right orchidectomy of which histopathologically confirmed to be a paratesticular AAM with clear resection margins...
April 2017: Urology Annals
https://www.readbyqxmd.com/read/28479777/one-year-survivor-of-metastatic-renal-cell-carcinoma-treated-with-pazopanib
#11
Fam Xeng Inn, Nurzarina Ahmed, Erica Yee Hing, Mohd Hafidzul Jasman
Tyrosine kinase inhibitor (TKI) and its side effects are well known. However, these are mainly descriptive, with pictorial data lacking. Here, in we report a case of metastatic renal cell carcinoma, treated with TKI, with classic side effects; supplemented with images that demonstrate the adverse effects of the drug. In addition, we discuss and demonstrate the computed tomography changes.
April 2017: Urology Annals
https://www.readbyqxmd.com/read/28475704/combined-fundus-autofluorescence-and-near-infrared-reflectance-as-prognostic-biomarkers-for-visual-acuity-in-foveal-sparing-geographic-atrophy
#12
Moritz Lindner, Jennifer Nadal, Matthias M Mauschitz, Anna Lüning, Joanna Czauderna, Maximilian Pfau, Steffen Schmitz-Valckenberg, Frank G Holz, Matthias Schmid, Monika Fleckenstein
Purpose: To identify predictors of best corrected visual acuity (BCVA) in eyes with foveal-sparing geographic atrophy (GA) secondary to age-related macular degeneration (AMD). Methods: Best corrected visual acuity (Early Treatment Diabetic Retinopathy Study charts); serial fundus autofluorescence; and near-infrared reflectance images of patients participating in the FAM (NCT00393692) and DSGA (NCT02051998) studies were analyzed. The sizes of GA and spared fovea, and the minimal linear dimension of intact retinal pigment epithelium ("bridge") between the residual foveal island and the surrounding retina were quantified and associations with BCVA were assessed by local regression curves and mixed effects models...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28472341/in-mammalian-foetal-testes-sox9-regulates-expression-of-its-target-genes-by-binding-to-genomic-regions-with-conserved-signatures
#13
Massilva Rahmoun, Rowena Lavery, Sabine Laurent-Chaballier, Nicolas Bellora, Gayle K Philip, Moïra Rossitto, Aleisha Symon, Eric Pailhoux, Florence Cammas, Jessica Chung, Stefan Bagheri-Fam, Mark Murphy, Vivian Bardwell, David Zarkower, Brigitte Boizet-Bonhoure, Philippe Clair, Vincent R Harley, Francis Poulat
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS)...
May 2, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28469188/the-first-late-triassic-chinese-triadophlebiomorphan-insecta-odonatoptera-biogeographic-implications
#14
Daran Zheng, André Nel, He Wang, Bo Wang, Edmund A Jarzembowski, Su-Chin Chang, Haichun Zhang
The clade Triadophlebiomorpha represents a morphological 'link' between the Paleozoic griffenflies (Meganisoptera) and the modern taxa. Nevertheless they are relatively poorly known in the body structures and paleobiogeography. The Triassic dragonfly is extremely rare in China with only one previously recorded. A new family, Sinotriadophlebiidae Zheng, Nel et Zhang fam. nov., for the genus and species Sinotriadophlebia lini Zheng, Nel et Zhang gen. et sp. nov., is described from the Upper Triassic Baijiantan Formation of Xinjiang, northwestern China...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28452376/a-new-approach-to-transfect-nf-%C3%AE%C2%BAb-decoy-oligodeoxynucleotides-into-the-periodontal-tissue-using-the-ultrasound-microbubble-method
#15
Hiroyuki Yamaguchi, Yuji Ishida, Jun Hosomichi, Jun-Ichi Suzuki, Risa Usumi-Fujita, Yasuhiro Shimizu, Sawa Kaneko, Takashi Ono
The objective of this study is to investigate the effect of the ultrasound-microbubble technique in nuclear factor kappa B (NF-κB) decoy oligodeoxynucleotide (ODN) transfection in the gingival tissue in mice. The 6-FAM-labeled scrambled decoy ODN with microbubbles was applied to the periodontal tissue in 8-week-old male C57BL/6J mice by ultrasound radiation at low (LUM-Sc) and high (HUM-Sc) intensities to optimize the transfection condition of the ultrasound-microbubble method. Histological inspections were performed two hours after transfection to compare the expression with that in the sham-operated group without ultrasound radiation (A-Sc)...
April 28, 2017: International Journal of Oral Science
https://www.readbyqxmd.com/read/28447873/employment-outcome-ten-years-after-moderate-to-severe-traumatic-brain-injury-a-prospective-cohort-study
#16
Erik Grauwmeijer, Majanka Heijenbrok-Kal, Iain Haitsma, Gerard Ribbers
The objective of this prospective cohort study was to evaluate the probability of employment and predictors of employment in patients with moderate to severe Traumatic Brain Injury (TBI) over 10 years follow-up. 113 patients (18-67 years) were included with follow-up measurements 3, 6, 12, 18, 24, 36 months and ten years after TBI. Potential predictors of employment probability included patient characteristics, injury severity factors, functional outcome measured at discharge from the hospital with the Glasgow Outcome Scale (GOS), Barthel Index (BI), Functional Independence Measure (FIM), and the Functional Assessment Measure (FAM)...
April 27, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28441340/selection-characterization-and-interaction-studies-of-a-dna-aptamer-for-the-detection-of-bifidobacterium-bifidum
#17
Lujun Hu, Linlin Wang, Wenwei Lu, Jianxin Zhao, Hao Zhang, Wei Chen
A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. After 12 rounds of selection targeted against B. bifidum, 30 sequences were obtained and divided into seven families according to primary sequence homology and similarity of secondary structure. Four FAM (fluorescein amidite) labeled aptamer sequences from different families were selected for further characterization by flow cytometric analysis...
April 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28436517/real-time-measurement-of-the-intracellular-ph-of-yeast-cells-during-glucose-metabolism-using-ratiometric-fluorescent-nanosensors
#18
Mohamed M Elsutohy, Veeren M Chauhan, Robert Markus, Mohammed Aref Kyyaly, Saul J B Tendler, Jonathan W Aylott
Intracellular pH is a key parameter that influences many biochemical and metabolic pathways that can also be used as an indirect marker to monitor metabolic and intracellular processes. Herein, we utilise ratiometric fluorescent pH-sensitive nanosensors with an extended dynamic pH range to measure the intracellular pH of yeast (Saccharomyces cerevisiae) during glucose metabolism in real-time. Ratiometric fluorescent pH-sensitive nanosensors consisting of a polyacrylamide nanoparticle matrix covalently linked to two pH-sensitive fluorophores, Oregon green (OG) and 5(6)carboxyfluorescein (FAM), and a reference pH-insensitive fluorophore, 5(6)carboxytetramethylrhodamine (TAMRA), were synthesised...
May 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28430524/mirna-related-polymorphisms-in-mir-423-rs6505162-and-pex6-rs1129186-and-risk-of-esophageal-squamous-cell-carcinoma-in-an-iranian-cohort
#19
Ziba Nariman-Saleh-Fam, Milad Bastami, Mohammad Hossein Somi, Farkhondeh Behjati, Yaser Mansoori, Abdolreza Daraei, Zahra Saadatian, Lida Nariman-Saleh-Fam, Habibollah Mahmoodzadeh, Yashar Makhdoumi, Fatemeh Varshoee Tabrizi, Bahador Ebrahimi-Sharif, Azam Hezarian, Shahnaz Naghashi, Mohammad Reza Abbaszadegan, Javad Tavakkoly-Bazzaz
AIMS: Iran is located in the Asian esophageal cancer belt. It is a high-risk region for esophageal squamous cell carcinoma (ESCC). The extent to which genetic components, especially variants within miRNAs or their binding sites, contribute to risk of ESCC in the region is not yet fully understood. Herein, tests were done on an Iranian cohort to evaluate the association of miRNA-related polymorphisms in miR-423 (rs6505162) and peroxisomal biogenesis factor 6 (PEX6) (rs1129186 within a miR-149-5p-binding site) with risk of ESCC risk...
April 21, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28416893/letter-to-the-editor-are-the-doors-opened-to-a-genetic-based-algorithm-for-personalized-resistance-training
#20
G Monnerat-Cahli, D Paulúcio, R S Moura Neto, R Silva, Fams Pompeu, B Budowle, C G Santos
No abstract text is available yet for this article.
March 2017: Biology of Sport
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