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https://www.readbyqxmd.com/read/27926817/description-of-immundisolibacter-cernigliae-gen-nov-sp-nov-a-high-molecular-weight-polycyclic-aromatic-hydrocarbon-degrading-bacterium-within-the-class-gammaproteobacteria-and-proposal-of-immundisolibacterales-ord-nov-and-immundisolibacteraceae-fam-nov
#1
Elizabeth M Corteselli, Michael D Aitken, David R Singleton
The bacterial strain TR3.2T was isolated from aerobic bioreactor-treated soil from a polycyclic aromatic hydrocarbon (PAH)-contaminated site in Salisbury, North Carolina, USA. Strain TR3.2T was identified as a member of "Pyrene Group 2" or "PG2", a previously uncultivated cluster of organisms associated with the degradation of high-molecular-weight PAHs by stable-isotope probing. Based on its 16S rRNA gene sequence the strain was classified as a member of the class Gammaproteobacteria but possessed only 90...
December 7, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27925345/feasibility-and-short-term-impact-of-the-case-study-in-house-group-training-program-for-family-nursing-at-medical-facilities
#2
Akemi Yamazaki, Akemi Tsumura, Hiroko Mine, Chisato Kimura, Akemi Soeda, Kazumi Odatsu, Wataru Kiwado
The aim of this study was to evaluate the feasibility and short-term impact of case study training in family nursing care targeting midlevel nursing professionals. The intervention group participated in four 90-minute case study training sessions over 6 months, while the control group participated in two 90-minute lectures. Using primary outcome variables as evaluation indexes, we measured the participants' total scores on the Family Importance in Nursing Care Scale and 4 subitems 3 times (before, immediately after and 1 month after training) from May 2014 to March 2015 and then conducted 2-way repeated-measure analysis of variance...
December 7, 2016: International Journal of Nursing Practice
https://www.readbyqxmd.com/read/27922813/transcatheter-tricuspid-repair-with-mitraclip-for-severe-primary-tricuspid-regurgitation
#3
Neil P Fam, Kim A Connelly, Christoph Hammerstingl, Geraldine Ong, Anthony W A Wassef, Heather J Ross, Subodh Verma
Severe tricuspid regurgitation is an independent predictor of adverse outcomes, yet few patients undergo surgery and treatment with medical therapy is often inadequate. Recent studies have reported the use of the MitraClip system (Abbott Vascular) to treat secondary tricuspid regurgitation. We describe the first use of MitraClip to treat severe primary tricuspid regurgitation and right heart failure in a patient with previous cardiac transplantation and high surgical risk.
December 2016: Journal of Invasive Cardiology
https://www.readbyqxmd.com/read/27902348/micropepsis-pineolensis-gen-nov-sp-nov-a-mildly-acidophilic-alphaproteobacterium-isolated-from-a-poor-fen-and-proposal-of-micropepsaceae-fam-nov-within-micropepsales-ord-nov
#4
Austin B Harbison, Laiken E Price, Michael D Flythe, Suzanna L Brauer
An obligately anaerobic, mildly acidophilic, fermentative alphaproteobacterium, designated strain CS4 T, was isolated from an acidic, oligotrophic (nutrient-poor) poor fen located near Pineola, NC, USA. Cultures contained Gram-negative staining, slightly curved, non-motile, non-spore forming, non-prosthecate rods (0.1-0.4 µm in diameter and 0.4-4 µm long, depending, in part, on the growth substrate). Growth optima were 35° C (range 15 - 35°C), pH 5.6 (range of 5.0 - 6.8), and with 0-50 mM added NaCl (range 0-100 mM added NaCl)...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902282/defining-the-taxonomic-status-of-described-subdivision-3-acidobacteria-the-proposal-of-bryobacteraceae-fam-nov
#5
Svetlana N Dedysh, Irina S Kulichevskaya, Katharina J Huber, Jörg Overmann
Acidobacteria represent one of the highly diverse but poorly characterized phylogenetic groups of the domain Bacteria. The taxonomically described acidobacteria belong to 27 genera and 49 species, which represent subdivisions 1, 3, 4, 6, 8, 10 and 23 of this phylum. However, the corresponding family ranks have been defined only for some of these characterized microorganisms. Here, we suggest the establishment of a novel family, Bryobacteraceae fam. nov., to accommodate taxonomically described members of subdivision 3 Acidobacteria...
November 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902267/soortia-roseihalophila-gen-nov-sp-nov-a-new-taxon-isolated-from-a-travertine-spring-in-the-order-balneolales-and-description-of-soortiaceae-fam-nov
#6
Mohammad Ali Amoozegar, Javad Khansha, Maliheh Mehrshad, Seyed Abolhassan Shahzadeh Fazeli, Mohaddaseh Ramezani, Rafael de la Haba, Cristina Sánchez-Porro, Antonio Ventosa
A novel Gram-stain-negative, slightly halophilic motile curved rod with horseshoe shape, designated strain Bsw-2bT, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2bT belongs to the order Balneolales, showing 84.6% sequence similarity to Gracilimonas tropica DSM 19535T and 84.4 % and 83.9 % sequence similarity to Gracilimonas rosea CL-KR2T and Balneola vulgaris DSM 17893T, respectively. Besides, phenotypic and physiological features could clearly differentiate strain Bsw-2bT from species of the most closely related genera Gracilimonas, Balneola, Aliifodinibius and Fodinibius...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902209/fimbriiglobus-ruber-gen-nov-sp-nov-a-gemmata-like-planctomycete-from-sphagnum-peat-bog-and-the-proposal-of-gemmataceae-fam-nov
#7
Irina S Kulichevskaya, Anastasia A Ivanova, Olga I Baulina, W Irene C Rijpstra, Jaap S Sinninghe Damsté, Svetlana N Dedysh
An aerobic, budding, dark pink to red-pigmented bacterium was isolated from an acidic boreal Sphagnum peat bog and designated strain SP5T. Cells of this strain were non-motile spheres that were uniformly covered with crateriform pits and fimbria, and tended to form aggregates during growth in liquid media. Strain SP5T was capable of growth at pH values between 4.0 and 6.8 (optimum at pH 5.5-6.0) and at temperatures between 10 and 30oC (optimum 20-25oC). The preferred growth substrates were sugars and some heteropolysaccharides...
October 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27896897/expanded-clinical-use-of-everolimus-eluting-bioresorbable-vascular-scaffolds-for-treatment-of-coronary-artery-disease
#8
Roberto Diletti, Yuki Ishibashi, Cordula Felix, Yoshinobu Onuma, Shimpei Nakatani, Nicolas M van Mieghem, Eveliyn Regar, Marco Valgimigli, Peter P de Jaegere, Nienke van Ditzhuijzen, Jiang Ming Fam, Jurgen M R Ligthart, Mattie J Lenzen, Patrick W Serruys, Felix Zijlstra, Robert Jan van Geuns
BACKGROUND: Limited data are currently available on the performance of everolimus eluting bioresorbable vascular scaffold (BVS) for treatment of complex coronary lesions representative of daily practice. METHODS: This is a prospective, mono-center, single-arm study, reporting data after BVS implantation in patients presenting with stable, unstable angina, or non-ST segment elevation myocardial infarction caused by de novo stenotic lesions in native coronary arteries...
November 29, 2016: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/27896606/a-knowledge-ecologies-analysis-of-co-designing-water-and-sanitation-services-in-alaska
#9
Dena Fam, Zoë Sofoulis
Willingness to collaborate across disciplinary boundaries is necessary but not sufficient for project success. This is a case study of a transdisciplinary project whose success was constrained by contextual factors that ultimately favoured technical and scientific forms of knowledge over the cultural intelligence that might ensure technical solutions were socially feasible. In response to Alaskan Water and Sewer Challenge (AWSC), an international team with expertise in engineering, consultative design and public health formed in 2013 to collaborate on a two-year project to design remote area water and sanitation systems in consultation with two native Alaskan communities...
November 28, 2016: Science and Engineering Ethics
https://www.readbyqxmd.com/read/27891524/the-design-usability-and-feasibility-of-a-family-focused-diabetes-self-care-support-mhealth-intervention-for-diverse-low-income-adults-with-type-2-diabetes
#10
Lindsay Satterwhite Mayberry, Cynthia A Berg, Kryseana J Harper, Chandra Y Osborn
Family members' helpful and harmful actions affect adherence to self-care and glycemic control among adults with type 2 diabetes (T2D) and low socioeconomic status. Few family interventions for adults with T2D address harmful actions or use text messages to reach family members. Through user-centered design and iterative usability/feasibility testing, we developed a mHealth intervention for disadvantaged adults with T2D called FAMS. FAMS delivers phone coaching to set self-care goals and improve patient participant's (PP) ability to identify and address family actions that support/impede self-care...
2016: Journal of Diabetes Research
https://www.readbyqxmd.com/read/27886763/dna-covalently-linked-to-graphene-oxide-for-biotin-streptavidin-interaction-assay
#11
Yue He, Bining Jiao
Based on terminal protection of small molecule-linked DNA and the covalently linking DNA to graphene oxide (GO) strategy, a high resisting nonspecific probe displacement platform for small molecule-protein interaction assay is proposed in this work. Specifically, the small molecule-linked DNA (probe 1) can be protected from exonuclease-catalyzed digestion upon binding to the protein target of the small molecule, so the DNA strand may hybridize with another DNA strand (FAM and amino dual modified DNA, probe 2) that is previously covalently linked onto GO surface...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27885061/alemtuzumab-improves-quality-of-life-outcomes-compared-with-subcutaneous-interferon-beta-1a-in-patients-with-active-relapsing-remitting-multiple-sclerosis
#12
Rafael Arroyo González, Mariko Kita, Heidi Crayton, Eva Havrdova, David H Margolin, Stephen L Lake, Gavin Giovannoni
BACKGROUND: Alemtuzumab was superior on clinical and magnetic resonance imaging (MRI) outcomes versus subcutaneous interferon beta-1a in phase 3 trials in patients with relapsing-remitting multiple sclerosis. OBJECTIVE: To examine quality-of-life (QoL) outcomes in the alemtuzumab phase 3 trials. METHODS: Patients who were treatment naive (Comparison of Alemtuzumab and Rebif(®) Efficacy in Multiple Sclerosis I [CARE-MS I]) or had an inadequate response to prior therapy (CARE-MS II) received annual courses of alemtuzumab 12 mg/day at baseline (5 days) and Month 12 (3 days) or subcutaneous interferon beta-1a 44 µg three times/week...
November 24, 2016: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/27878128/the-epidemilogical-characteristics-of-motorcyclists-associated-injuries-in-road-traffics-accidents-a-hospital-based-study
#13
Saber Ghaffari-Fam, Ehsan Sarbazi, Amin Daemi, Mohammad Reza Sarbazi, Hossein Ali Nikbakht, Shaker Salarilak
OBJECTIVES: To determine the epidemiology of injuries of the motorcyclists in road traffic accidents (RTIs) between 2008 and 2014 in East Azarbayejan province of Iran. METHODS: A cross-sectional study was performed in East Azerbaijan province in Imam Reza hospital on motorcyclists subjected to injuries in RTIs between 2008 and 2014. Demographic characteristics (age, sex), duration of hospitalization, final status of injured people after discharge from the hospital, admission ward, number of admissions due to injuries in RTIs, nature of injury, and the clinical services provided to injured people were gathered from hospital information system (HIS)...
October 2016: Bulletin of Emergency and Trauma
https://www.readbyqxmd.com/read/27876876/fabrication-of-functional-hollow-microspheres-constructed-from-mof-shells-promising-drug-delivery-systems-with-high-loading-capacity-and-targeted-transport
#14
Xuechuan Gao, Xiao Hai, Huricha Baigude, Weihua Guan, Zhiliang Liu
An advanced multifunctional, hollow metal-organic framework (MOF) drug delivery system with a high drug loading level and targeted delivery was designed and fabricated for the first time and applied to inhibit tumour cell growth. This hollow MOF targeting drug delivery system was prepared via a simple post-synthetic surface modification procedure, starting from hollow ZIF-8 successfully obtained for the first time via a mild phase transformation under solvothermal conditions. As a result, the hollow ZIF-8 exhibits a higher loading capacity for the model anticancer drug 5-fluorouracil (5-FU)...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27871616/a-sensitive-fluorescence-biosensor-for-alkaline-phosphatase-activity-based-on-the-cu-ii-dependent-dnazyme
#15
Mengmeng Zhao, Yajuan Guo, Lixu Wang, Fang Luo, Cuiying Lin, Zhenyu Lin, Guonan Chen
Alkaline phosphatase (ALP) plays an important role in phosphate metabolism processes; deviation from its normal level may indicate different kinds of diseases, so it is highly necessary to develop some simple and sensitive methods to monitor the ALP level. In this study, a simple, high selective, and sensitive fluorescent biosensor has been proposed for ALP activity determination. The Cu(II)-dependent DNAzyme (Cu-Enzyme) are divided into two parts: Cu-Enzyme 1 and Cu-Enzyme 2, and labelled with alkyne and azido groups, respectively...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27869723/quencher-free-fluorescence-method-for-the-detection-of-mercury-ii-based-on-polymerase-aided-photoinduced-electron-transfer-strategy
#16
Haisheng Liu, Linbin Ma, Changbei Ma, Junyan Du, Meilan Wang, Kemin Wang
A new quencher-free Hg(2+) ion assay method was developed based on polymerase-assisted photoinduced electron transfer (PIET). In this approach, a probe is designed with a mercury ion recognition sequence (MRS) that is composed of two T-rich functional areas separated by a spacer of random bases at the 3'-end, and a sequence of stacked cytosines at the 5'-end, to which a fluorescein (FAM) is attached. Upon addition of Hg(2+) ions into this sensing system, the MRS folds into a hairpin structure at the 3'-end with Hg(2+)-mediated base pairs...
November 18, 2016: Sensors
https://www.readbyqxmd.com/read/27861701/preparing-family-caregivers-to-recognize-delirium-symptoms-in-older-adults-after-elective-hip-or-knee-arthroplasty
#17
Margaret J Bull, Lesley Boaz, Mehdi Maadooliat, Mary E Hagle, Lynn Gettrust, Maureen T Greene, Sue Baird Holmes, Jane S Saczynski
OBJECTIVES: To test the feasibility of a telephone-based intervention that prepares family caregivers to recognize delirium symptoms and how to communicate their observations to healthcare providers. DESIGN: Mixed-method, pre-post quasi-experimental design. SETTING: A Midwest Veterans Affairs Medical Center and a nonprofit health system. PARTICIPANTS: Forty-one family caregiver-older adult dyads provided consent; 34 completed the intervention...
November 11, 2016: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/27859383/design-installation-and-performance-evaluation-of-a-custom-dye-matrix-standard-for-automated-capillary-electrophoresis
#18
Kevin Wesley Cloete, Peter Gustav Ristow, Mohaimin Kasu, Maria Eugenia D'Amato
Capillary electrophoresis equipment detects and deconvolutes mixtures containing up to six fluorescently labelled DNA fragments. This deconvolution is done by the collection software which requires a spectral calibration file. The calibration file is used to adjust for the overlap that occurs between the emission spectra of fluorescence dyes. All commercial genotyping and sequencing kits require the installation of a corresponding matrix standard to generate a calibration file. Due to the differences in emission spectrum overlap between fluorescent dyes, the application of existing commercial matrix standards to the electrophoretic separation of DNA labelled with other fluorescent dyes can yield undesirable results...
November 10, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27856713/effect-of-increased-and-maintained-frequency-of-speed-endurance-training-on-performance-and-muscle-adaptations-in-runners
#19
Casper Skovgaard, Nicki Winfield Almquist, Jens Bangsbo
The aim of the study was, in runners accustomed to speed endurance training (SET), to examine the effect of increased and maintained frequency of SET on performance and muscular adaptations. After familiarization (FAM) to SET, eighteen male (n=14) and female (n=4) runners (VO2-max: 57.3±3.4 ml·min(-1); mean±SD) completed twenty sessions of maintained low-frequency (LF; every fourth day; n=7) or high-frequency (HF; every second day; n=11) SET (intervention period; INT). Before FAM as well as before and after INT, subjects completed a series of running tests and a biopsy from m...
November 17, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27852341/larval-digenean-preferences-in-two-sympatric-snail-species-at-differing-tidal-levels-off-the-atlantic-coast-of-patagonia
#20
G Di Giorgio, C Gilardoni, E Bagnato, F Cremonte, C Ituarte
Larval stages of the trematodes Maritrema madrynense and Hemiuroidea fam. gen. et sp. differentially parasitized Siphonaria lessonii and S. lateralis, two sympatric pulmonate snail species on the rocky intertidal shore at Puerto Deseado, south-western Atlantic coast of Patagonia, Argentina. Snail specimens were collected at two sampling sites with contrasting physical-chemical characteristics. One site, in the upper intertidal, was exposed to sewage from fish-processing plants, greater hydrodynamic forcing and desiccation, a wider temperature range, longer exposure to ultraviolet radiation and higher abundance of birds...
November 17, 2016: Journal of Helminthology
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