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https://www.readbyqxmd.com/read/29332829/synchronous-detection-of-ebolavirus-conserved-rna-sequences-and-ebolavirus-encoded-mirna-like-fragment-based-on-a-zwitterionic-copper-ii-metal-organic-framework
#1
Gui-Hua Qiu, Zi-Hua Weng, Pei-Pei Hu, Wen-Jun Duan, Bao-Ping Xie, Bin Sun, Xiao-Yan Tang, Jin-Xiang Chen
From a three-dimensional (3D) metal-organic framework (MOF) of {[Cu(Cmdcp)(phen)(H2O)]2·9H2O}n (1, H3CmdcpBr = N-carboxymethyl-(3,5-dicarboxyl)pyridinium bromide, phen = phenanthroline), a sensitive and selective fluorescence sensor has been developed for the simultaneous detection of ebolavirus conserved RNA sequences and ebolavirus-encoded microRNA-like (miRNA-like) fragment. The results from molecular dynamics simulation confirmed that MOF 1 absorbs carboxyfluorescein (FAM)-tagged and 5(6)-carboxyrhodamine, triethylammonium salt (ROX)-tagged probe ss-DNA (probe DNA, P-DNA) by π…π stacking and hydrogen bonding, as well as additional electrostatic interactions to form a sensing platform of P-DNAs@1 with quenched FAM and ROX fluorescence...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29330848/cultured-allogeneic-fibroblast-injection-versus-fibroblasts-cultured-on-amniotic-membrane-scaffold-for-dystrophic-epidermolysis-bullosa-treatment
#2
H Moravvej, F Abdollahimajd, M-H Naseh, Z Piravar, E Abolhasani, N Mozafari, H Niknejad
BACKGROUND: Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB). OBJECTIVE: To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with that of fibroblasts seeded on amniotic membrane scaffolds (FAMS) or standard wound care (SWC) with Vaseline gauze as controls. MATERIALS &METHODS: Seven patients were recruited, and seven wounds were assessed in each patient: three wounds were treated with injection of intradermal fibroblasts, three were treated with FAMS, and one was dressed with SWC...
January 12, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29327315/maternal-bonding-behavior-adult-intimate-relationship-and-quality-of-life
#3
Michaela Schmoeger, Matthias Deckert, Petra Wagner, Ulrike Sirsch, Ulrike Willinger
Continuity and discontinuity in the development of social relationships have been investigated by reviewing the course of social bonds and by analyzing the effects of a sound intimate relationship in adulthood in conjunction with recalled maternal bonding on the quality of life among students.A questionnaire-based study of 207 students was conducted. Perceptions of maternal bonding were designated as being representative of one of the two contrasting bonding types "optimal maternal bonding" and "affectionless maternal control" assessed by the Parental Bonding Instrument (PBI) and combined with perceptions of a sound intimate adult relationship measured by the Family Assessment Measure III Dyadic Relationships Scale (FAM-III-D)...
January 11, 2018: Neuropsychiatrie: Klinik, Diagnostik, Therapie und Rehabilitation
https://www.readbyqxmd.com/read/29325988/arboriscoccus-pini-gen-nov-sp-nov-an-endophyte-from-a-pine-tree-of-the-class-alphaproteobacteria-emended-description-of-geminicoccus-roseus-and-proposal-of-geminicoccaceae-fam-nov
#4
Diogo N Proença, William B Whitman, Neha Varghese, Nicole Shapiro, Tanja Woyke, Nikos C Kyrpides, Paula V Morais
Bacterial strain B29T1T was isolated from the endophytic microbial community of a Pinus pinaster tree trunk and characterized. Strain B29T1T stained Gram-negative and formed diplococci that grew optimally at 26-30°C and at pH 6.0-7.0. The G+C content of the DNA was 61.6mol%. The respiratory quinone was ubiquinone 10 (UK-10), and the major fatty acids were C16:0, cyclo-C19:0ω8c and C18:0 12-methyl, representing 64% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain B29T1T within the order Rhodospirillales in a distinct lineage that also included the genus Geminicoccus...
December 22, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29322298/a-new-microsporidium-fibrillaspora-daphniae-g-n-sp-n-infecting-daphnia-magna-crustacea-cladocera-in-siberia-and-its-taxonomic-placing-within-a-new-family-fibrillasporidae-and-new-superfamily-tubulinosematoidea-opisthosporidia-microsporidia
#5
Anastasia V Simakova, Yuri S Tokarev, Irma V Issi
Infection with a new microsporidium, Fibrillaspora daphniae g. n. sp. n., was found in a local Daphnia magna population in Tomsk region (Western Siberia, Russia) at the prevalence rate of 52%. Histological sections showed parasite cells entirely encompassing the host haemocoel. Methanol-fixed spores were elongate, oval, 4.8 ± 0.3 μm × 2.3 ± 0.2 μm in size. All developmental stages were in direct contact with the host cell cytoplasm, with single nuclei, and division by binary fission. The sporont surface was covered with an additional outer layer composed of fine tubules...
January 10, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29310291/fluorescence-sensing-platform-based-on-ruthenium-ii-complexes-as-high-3s-sensitivity-specificity-speed-and-on-off-on-sensors-for-the-mir-185-detection
#6
Bin Sun, Zhen Liang, Bao-Ping Xie, Rong-Tian Li, Lin-Ze Li, Zhi-Hong Jiang, Li-Ping Bai, Jin-Xiang Chen
Inspired by the enormous importance attributed to the biological function of miRNA, we pour our attention into the design and synthesis of four ruthenium(II) complexes and evaluate their applications as miR-185 detection agents by spectroscopic measurements. It was found that all complexes can form sensing platform for the detection of the complementary target miR-185 through the introduction of carboxyfluorescein (FAM) labeled single stranded DNA (P-DNA), giving the detection limits of 0.42nM for Ru 1, 0.28nM for Ru 2, 0...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310271/a-novel-peptide-fe3o4-sio2-au-nanocomposite-based-fluorescence-biosensor-for-the-highly-selective-and-sensitive-detection-of-prostate-specific-antigen
#7
Lanfang Yang, Nan Li, Ke Wang, Xiaoman Hai, Junxia Liu, Fuquan Dang
Highly selective and sensitive detection methods are very important for the early diagnosis of prostate-specific antigen (PSA). Here, we present a novel peptide/Fe3O4@SiO2-Au nanocomposite-based fluorescence biosensor for highly selective and sensitive detection of PSA. The biosensor was made by self-organizing 5-FAM labeled peptides onto the surface of magnetic Fe3O4@SiO2-Au nanocomposites (MNCPs), resulting in efficient quenching of the FAM fluorescence. The PSA specifically recognized and cleaved the 5-FAM-labeled peptides, leading to the fluorescence recovery...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310233/a-fluorescent-on-off-on-probe-for-sensitive-detection-of-atp-based-on-atp-displacing-dna-from-nanoceria
#8
Fangfang Li, Xuan Hu, Fengyi Wang, Baozhan Zheng, Juan Du, Dan Xiao
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29299847/baekduia-soli-gen-nov-sp-nov-a-novel-bacterium-isolated-from-the-soil-of-baekdu-mountain-and-proposal-of-a-novel-family-name-baekduiaceae-fam-nov
#9
Dong-Shan An, Muhammad Zubair Siddiqi, Kyoung-Ho Kim, Hong-Shan Yu, Wan-Taek Im
A taxonomic study was conducted on BR7-21T, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684T, Solirubrobacter pauli ATCC BAA-492T, Patulibacter minatonensis JCM 12834T, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21T was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29258538/predominance-of-girls-with-cancer-in-families-with-multiple-childhood-cancer-cases
#10
Karl-Johan Stjernfelt, Kristoffer von Stedingk, Thomas Wiebe, Lars Hjorth, Håkan Olsson, Ingrid Øra
BACKGROUND: Recent studies indicate that one of four childhood cancers can be attributed to hereditary genetic abnormalities. METHODS: The Lund Childhood Cancer Genetic study includes newly diagnosed childhood cancer patients as well as childhood cancer survivors visiting the Department of Pediatrics or the Late Effect Clinic at Skåne University Hospital, Lund, Sweden. Questionnaires regarding family history of cancer and blood samples were provided. Reported data were validated and extended by use of the Swedish Population- and Cancer Registries...
December 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29246307/an-albumin-oligonucleotide-assembly-for-potential-combinatorial-drug-delivery-and-half-life-extension-applications
#11
Matthias Kuhlmann, Jonas B R Hamming, Anders Voldum, Georgia Tsakiridou, Maja T Larsen, Julie S Schmøkel, Emil Sohn, Konrad Bienk, David Schaffert, Esben S Sørensen, Jesper Wengel, Daniel M Dupont, Kenneth A Howard
The long blood circulatory property of human serum albumin, due to engagement with the cellular recycling neonatal Fc receptor (FcRn), is an attractive drug half-life extension enabling technology. This work describes a novel site-specific albumin double-stranded (ds) DNA assembly approach, in which the 3' or 5' end maleimide-derivatized oligodeoxynucleotides are conjugated to albumin cysteine at position 34 (cys34) and annealed with complementary strands to allow single site-specific protein modification with functionalized ds oligodeoxynucleotides...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29245386/revisionary-systematics-of-the-endemic-soft-coral-fauna-octocorallia-alcyonacea-alcyoniina-of-the-agulhas-bioregion-south-africa
#12
Catherine S McFadden, Leen P VAN Ofwegen
The Agulhas Bioregion of southern South Africa supports a unique octocoral fauna that is entirely endemic to the region. Recent molecular phylogenetic studies indicate that this endemism extends to deeper taxonomic levels than had previously been recognized, and that most of these endemic species belong to genera and families that are also endemic to South Africa. Here we revise the taxonomy of soft corals (subordinal group Alcyoniina) that were previously assigned to the cosmopolitan families Alcyoniidae, Nephtheidae and Nidaliidae, describing three new families endemic to South Africa, along with four new genera and four new species...
December 12, 2017: Zootaxa
https://www.readbyqxmd.com/read/29242774/multigene-phylogeny-of-endogonales-an-early-diverging-lineage-of-fungi-associated-with-plants
#13
Alessandro Desirò, William R Rimington, Alison Jacob, Natalie Vande Pol, Matthew E Smith, James M Trappe, Martin I Bidartondo, Gregory Bonito
Endogonales is a lineage of early diverging fungi within Mucoromycota. Many species in this order produce small sporophores ("sporocarps") containing a large number of zygospores, and many species form symbioses with plants. However, due to limited collections, subtle morphological differentiation, difficulties in growing these organisms in vitro, and idiosyncrasies in their rDNA that make PCR amplification difficult, the systematics and character evolution of these fungi have been challenging to resolve. To overcome these challenges we generated a multigene phylogeny of Endogonales using sporophores collected over the past three decades from four continents...
December 2017: IMA Fungus
https://www.readbyqxmd.com/read/29241186/strengthening-gastric-cancer-therapy-by-trastuzumab-conjugated-nanoparticles-with-simultaneous-encapsulation-of-anti-mir-21-and-5-fluorouridine
#14
Nan Hu, Jun Feng Yin, Ze Ji, Yidong Hong, Puyuan Wu, Baoxiang Bian, Ziyan Song, Rutian Li, Qin Liu, Fenglei Wu
BACKGROUND/AIMS: MicroRNA-21 is an oncogenic miR (oncomiR) frequently elevated in gastric cancer (GC). Overexpression of miR-21 decreases the sensitivity of GC cells to 5-fluorouridine (5-Fu) and trastuzumab, a humanized monoclonal antibody targeting human epidermal growth factor receptor 2 (HER2). Receptor-mediated endocytosis plays a crucial role in the delivery of biotherapeutics including anti-miRNA oligonucleotides (AMOs). This study is a continuation of earlier findings involving poly(ε-caprolactone) (PCL)-poly (ethylene glycol) (PEG) nanoparticles (PEG-PCL NPs), which were coated with trastuzumab to target GC with HER2 receptor over-expression using anti-miRNA-21 (AMO-21) and 5-Fu...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29233678/detection-of-simultaneous-multi-mutations-using-base-quenched-probe
#15
Huihui Mao, Guanghua Luo, Jun Zhang, Ning Xu
The base-quenched probe method for detecting single nucleotide polymorphisms (SNPs) relies on real-time PCR and melting-curve approaches. Here, we applied the most common commercial fluorophores including FAM, HEX, CY5, CY3, TET, JOE, Texas Red and ROX for labeling probes to detect multi-mutations simultaneously according to the different fluorescence channels. Accuracy of the method was confirmed by direct sequencing. The results demonstrated that all above dyes could be influenced by bases and could be applied to detect SNPs...
December 9, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29224176/validation-of-a-method-for-estimating-energy-expenditure-during-walking-in-middle-aged-adults
#16
Nathan Caron, Teddy Caderby, Nicolas Peyrot, Chantal Verkindt, Georges Dalleau
PURPOSE: The aim of this study was to test the validity of a method using an inertial measurement unit for estimating activity-related energy expenditure (AEE) during walking in middle-aged adults. METHODS: Twenty healthy middle-aged participants completed different treadmill walking trials with an inertial measurement unit adhered to their lower back. Gas exchange was monitored with indirect calorimetry. Mechanical data were used to estimate AEE from an algorithm developed by Bouten et al...
December 9, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29222904/dietary-patterns-change-over-two-years-in-early-adolescent-girls-in-hawai-i
#17
Michelle Ann Mosley, Jinan C Banna, Eunjung Lim, Marie Kainoa Fialkowski, Rachel Novotny
BACKGROUND AND OBJECTIVES: In investigating diet-disease relationships, examination of dietary patterns allows for conclusions to be drawn based on overall intake. This study characterized dietary patterns of early adolescent girls over a two-year period and examined the relationship between dietary patterns and body mass index (BMI). METHODS AND STUDY DESIGN: Cross-sectional analyses were performed using longitudinal data from food records of early adolescent girls (n=148) 9 to 14 years in Hawai'i from the Female Adolescent Maturation (FAM) study...
2018: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29215269/lanthanum-based-metal-organic-frameworks-for-specific-detection-of-sudan-virus-rna-conservative-sequences-down-to-single-base-mismatch
#18
Shui-Ping Yang, Wei Zhao, Pei-Pei Hu, Ke-Yang Wu, Zhi-Hong Jiang, Li-Ping Bai, Min-Min Li, Jin-Xiang Chen
Reactions of La(NO3)3·6H2O with the polar, tritopic quaternized carboxylate ligands N-carboxymethyl-3,5-dicarboxylpyridinium bromide (H3CmdcpBr) and N-(4-carboxybenzyl)-3,5-dicarboxylpyridinium bromide (H3CbdcpBr) afford two water-stable metal-organic frameworks (MOFs) of {[La4(Cmdcp)6(H2O)9]}n (1, 3D) and {[La2(Cbdcp)3(H2O)10]}n (2, 2D). MOFs 1 and 2 absorb the carboxyfluorescein (FAM)-tagged probe DNA (P-DNA) and quench the fluorescence of FAM via a photoinduced electron transfer (PET) process. The nonemissive P-DNA@MOF hybrids thus formed in turn function as sensing platforms to distinguish conservative linear, single-stranded RNA sequences of Sudan virus with high selectivity and low detection limits of 112 and 67 pM, respectively (at a signal-to-noise ratio of 3)...
December 7, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29205156/edge-to-edge-repair-of-a-large-anterior-leaflet-notch-with-severe-tricuspid-regurgitation
#19
Neil P Fam, Edwin C Ho, Jeremy Edwards, Kim A Connelly
No abstract text is available yet for this article.
December 5, 2017: EuroIntervention
https://www.readbyqxmd.com/read/29204748/imaging-the-expression-of-glypican-3-in-hepatocellular-carcinoma-by-pet
#20
Zhen Wang, Yan-Jiang Han, Shun Huang, Meng Wang, Wen-Lan Zhou, Hong-Sheng Li, Quan-Shi Wang, Hu-Bing Wu
The glypican-3 (GPC3) receptor is overexpressed in hepatocellular carcinoma (HCC) and is a potential diagnostic and therapeutic target. GPC3-targeted molecular imaging will be helpful to differentiate diagnosis and guide therapy. In the present study, we will develop a novel PET probe for imaging the expression of GPC-3. L5 (sequence: RLNVGGTYFLTTRQ), a GPC3 targeting peptide, was labeled with 5-carboxyfluorescein (FAM) and 18F-fluoride. Cell binding tests were performed to identify the binding specificity of FAM-L5 and 18F radiolabeled peptide...
December 4, 2017: Amino Acids
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