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https://www.readbyqxmd.com/read/28225583/microfluidic-encapsulation-of-human-mesenchymal-stem-cells-for-articular-cartilage-tissue-regeneration
#1
Fanyi Li, Vinh X Truong, Helmut Thissen, Jessica Frith, John S Forsythe
Stem cell injections for the treatment of articular cartilage damage are a promising approach to achieve tissue regeneration. However, this method is encumbered by high cell apoptosis rates, low retention in the cartilage lesion and inefficient chondrogenesis. Here, we have used a facile, very low cost-based microfluidic technique to create visible light-cured hydrogel microparticles composed of gelatin norbornene (GelNB) and a polyethylene glycol (PEG) crosslinker. In addition, we have demonstrated that the process enables the rapid in situ micro-encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs) under biocompatible microfluidic-processing conditions for long-term maintenance...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28222888/acinar-cell-response-to-liquid-diet-during-rats-growth-period-differs-in-submandibular-and-sublingual-glands-from-that-in-parotid-glands
#2
Shigeru Takahashi, Hiroki Uekita, Hiroto Taniwaki, Takanori Domon
Continuously feeding a liquid diet to growing rodents strongly inhibits parotid gland growth, due to suppressed growth of acinar cells. This study investigated whether a liquid diet had a similar effect on submandibular and sublingual glands of growing rats. Rats were weaned on day 21 after birth and then fed a pellet diet in the control group and a liquid diet in the experimental group for 0, 1, 2, 4, and 8 weeks. Their submandibular and sublingual glands were excised, weighed, and examined histologically, immunohistochemically (using antibodies to 5'-bromo-2-deoxyuridine and cleaved caspase 3), and ultrastructurally...
February 8, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28222838/prevalence-of-food-impaction-induced-periodontitis-in-conventionally-housed-marsh-rice-rats-oryzomys-palustris
#3
Jonathan G Messer, Jessica M Jiron, Hung-Yuan Chen, Evelyn J Castillo, Jorge L Mendieta Calle, Mary K Reinhard, Donald B Kimmel, J Ignacio Aguirre
Marsh rice rats (Oryzomys palustris) fed a pelleted diet high in sucrose and casein have been used as a model for moderate to severe periodontitis. Here we characterize the prevalence, location, and histopathologic features of food-impaction lesions (FIL), a unique type of oral event, in rice rats fed standard pelleted rodent chow from weaning until 34 wk of age. Healthy female rats (n = 90; age, 4 wk) were weaned into groups (n = 10 to 24) and were euthanized at 4, 16, 22, 28, or 34 wk of age. At necropsy, high-resolution photographs of the 4 jaw quadrants were examined by 3 independent observers to determine the presence, number, and location of FIL...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28222835/clinical-assessment-of-urinary-tract-damage-during-sustained-release-estrogen-supplementation-in-mice
#4
Dalis E Collins, Kathleen R Mulka, Mark J Hoenerhoff, Russell S Taichman, Jason S Villano
Estrogen supplementation is a key component of numerous mouse research models but can adversely affect the urinary system. The goal of this study was to develop a clinical scoring system and identify biomarkers of occult urinary tract lesions prior to the development of systemic illness in mice. Ovariectomized or sham-surgery SCID mice were implanted subcutaneously with a placebo pellet or one containing sustained-release estradiol (0.18 mg 60-d release 17β-estradiol). Mice were assessed twice weekly for 4 to 6 wk by using a clinical scoring system that included body condition, general activity, posture, hair coat, hydration, abdominal distension, urine staining of coat and skin, and ability to urinate...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28222133/maternal-psychological-stress-induced-developmental-disability-neonatal-mortality-and-stillbirth-in-the-offspring-of-wistar-albino-rats
#5
Sakthivel Govindaraj, Annadurai Shanmuganathan, Ravindran Rajan
BACKGROUND: Stress is an inevitable part of life, and maternal stress during the gestational period has dramatic effects in the early programming of the physiology and behavior of offspring. The developmental period is crucial for the well-being of the offspring. Prenatal stress influences the developmental outcomes of the fetus, in part because the developing brain is particularly vulnerable to stress. The etiology of birth defects of the offspring is reported to be 30-40% genetic and 7-10% multifactorial, with the remaining 50% still unknown and also there is no clear cause for neonatal mortality and still-birth...
2017: PloS One
https://www.readbyqxmd.com/read/28221914/effects-of-inoculation-procedures-on-variability-and-repeatability-of-salmonella-thermal-resistance-in-wheat-flour
#6
Ian M Hildebrandt, Bradley P Marks, Elliot T Ryser, Rossana Villa-Rojas, Juming Tang, Francisco J Garces-Vega, Sarah E Buchholz
Limited prior research has shown that inoculation methods affect thermal resistance of Salmonella in low-moisture foods; however, these effects and their repeatability have not been systematically quantified. Consequently, method variability across studies limits utility of individual data sets and cross-study comparisons. Therefore, the objective was to evaluate the effects of inoculation methodologies on stability and thermal resistance of Salmonella in a low-moisture food (wheat flour), and the repeatability of those results, based on data generated by two independent laboratories...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221079/cue-induced-food-seeking-after-punishment-is-associated-with-increased-fos-expression-in-the-lateral-hypothalamus-and-basolateral-and-medial-amygdala
#7
Erin J Campbell, David J Barker, Helen M Nasser, Konstantin Kaganovsky, Christopher V Dayas, Nathan J Marchant
In humans, relapse to unhealthy eating habits following dieting is a significant impediment to obesity treatment. Food-associated cues are one of the main triggers of relapse to unhealthy eating during self-imposed abstinence. Here we report a behavioral method examining cue-induced relapse to food seeking following punishment-induced suppression of food taking. We trained male rats to lever press for food pellets that were delivered after a 10-s conditional stimulus (CS) (appetitive). Following training, 25% of reinforced lever presses resulted in the presentation of a compound stimulus consisting of a novel CS (aversive) and the appetitive CS followed by a pellet and footshock...
February 20, 2017: Behavioral Neuroscience
https://www.readbyqxmd.com/read/28219467/comparative-fibre-degrading-capacity-in-foals-at-immediate-and-late-post-weaning-periods
#8
C Faubladier, V Julliand, L Beuneiche, C Philippeau
Total tract apparent digestibility of dietary fibrous components and parameters of the faecal digestive ecosystem were compared at immediate and late post-weaning periods using five foals. The foals were abruptly weaned (day 0) from their mares at 6 months of age. Immediately (day 0 to day 14) and later (day 131 to day 194) after weaning, foals received the same basal diet consisting of hay and pellets. Pellets were fed at 0.36% BW. Hay was offered ad libitum and corresponded to 120% of the average voluntary hay intake measured individually during the immediate (days 1 to 4) and late (days 180 to 183) post-weaning periods...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28215861/effects-of-anthelmintic-treatment-and-feed-supplementation-on-parasite-infections-and-morbidity-parameters-in-cambodian-cattle
#9
Veronique Dermauw, Sothy Meas, Bunthon Chea, Thierry Onkelinx, San Sorn, Davun Holl, Johannes Charlier, Jozef Vercruysse, Pierre Dorny
Helminth infections are the cause of morbidity in Cambodian cattle but other factors such as nutritional deficiencies and concurrent diseases may enhance the effects of parasites. The present study aimed to investigate the impact of anthelmintic treatment, feed supplementation, or both on gastrointestinal strongyle (GIS) and trematode infections as well as on morbidity parameters in Cambodian village cattle. At the beginning of the dry season, cattle populations in six villages were randomly assigned to a group: (A) receiving anthelmintic treatment (ivermectin+clorsulon) at week 0; (P) feed pellet supplementation during week 0-13 or both (AP)...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215185/nutritive-value-and-anthelmintic-effect-of-sainfoin-pellets-fed-to-experimentally-infected-growing-rabbits
#10
H Legendre, H Hoste, T Gidenne
Alternative strategies to synthetic chemical drugs are needed in livestock and are a key issue in organic farming today. This study aimed at examining the potentialities of sainfoin, a legume rich in condensed tannins, as a nutraceutical that combines nutritive and antiparasitic effects in rabbits. To test the effect of infection with a helminth (I: infected groups; NI: not infected groups) and the effect of substituting 40% of the alfalfa in a control diet (C) with sainfoin (diet S), four groups of 16 weaned rabbits were arranged according to a 2×2 bifactorial design...
February 20, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28212707/astrobiological-implications-of-dim-light-phototrophy-in-deep-sea-red-clays
#11
Anindita Das, Tanya Singh, P A LokaBharathi, Prashant K Dhakephalkar, Sweta Mallik, Pranav R Kshirsagar, N H Khadge, B Nagender Nath, Satadru Bhattacharya, Aditya Kumar Dagar, Prabhjot Kaur, Dwijesh Ray, Anil D Shukla, Christabelle E G Fernandes, Sheryl O Fernandes, Tresa Remya A Thomas, Mamatha S S, Babu Shashikant Mourya, Ram Murti Meena
Red clays of Central Indian Basin (CIB) under influence of trace of Rodriguez Triple Junction exhibited chemoautotrophy, low temperature hydrothermal alterations and photoautotrophic potential. Seamount flank TVBC-08, hosting such signatures revealed dominance of aerobic anoxygenic phototroph Erythrobacter, with 93% of total 454 pyrosequencing tags. Subsequently, enrichments for both aerobic (Erythrobacter) and anaerobic anoxygenic phototrophs (green and purple sulphur bacteria) under red and white LED light illumination, with average irradiance 30...
February 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28210781/adolescent-cocaine-exposure-enhances-goal-tracking-behavior-and-impairs-hippocampal-cell-genesis-selectively-in-adult-bred-low-responder-rats
#12
M Julia García-Fuster, Aram Parsegian, Stanley J Watson, Huda Akil, Shelly B Flagel
RATIONALE: Environmental challenges during adolescence, such as drug exposure, can cause enduring behavioral and molecular changes that contribute to life-long maladaptive behaviors, including addiction. Selectively bred high-responder (bHR) and low-responder (bLR) rats represent a unique model for assessing the long-term impact of adolescent environmental manipulations, as they inherently differ on a number of addiction-related traits. bHR rats are considered "addiction-prone," whereas bLR rats are "addiction-resilient," at least under baseline conditions...
February 16, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28207756/structural-changes-on-a-molecular-basis-of-canola-meal-by-conditioning-temperature-and-time-during-pelleting-process-in-relation-to-physiochemical-energy-and-protein-properties-relevant-to-ruminants
#13
Xuewei Huang, Huihua Zhang, Peiqiang Yu
The objectives of this study were: (1) To investigate the effects of conditioning temperature (70, 80, 90°C), time (30, 60 sec), and interaction (temperature × time) during the pelleting process on internal protein molecular structure changes of the co-products; (2) To identify differences in protein molecular structures among pellets that were processed under different conditions, and between unprocessed mash and pellets; 3) To quantify protein molecular structure changes in relation to predicted energy and protein utilization in dairy cows...
2017: PloS One
https://www.readbyqxmd.com/read/28206950/use-of-pellet-guns-for-crowd-control-in-kashmir-how-lethal-is-non-lethal
#14
Siddarth David
The use of pellet guns during the recent unrest in Kashmir as a method of crowd control has been questioned because of several deaths and numerous injuries. Across the world, these rubber pellets have been shown to inflict serious injuries, permanent disability, and death. The volatility of mob violence, inaccuracies in aim of the pellets, over-use of the pellet guns, and the perception of their harmlessness enhances the destructive potential of these so-called non-lethal weapons. There is also the larger ethical question whether any form of pain, however minimal, could be inflicted to control violent crowds...
December 13, 2016: Indian Journal of Medical Ethics
https://www.readbyqxmd.com/read/28206880/determination-of-zn-in-dry-feeds-for-cats-and-dogs-by-energy-dispersive-x-ray-fluorescence-spectrometry
#15
Dayara Virgnía L Ávila, Sidnei O Souza, Silvânio Silvério L Costa, Rennan Geovanny O Araujo, Carlos Alexandre B Garcia, José do Patrocínio H Alves, Elisangela A Passos
This work describes an analytical method for Zn determination in dry feeds for cats and dogs by energy-dispersive X-ray fluorescence (EDXRF). Samples of dry feed were powdered and prepared in the form of pellets for direct analysis by EDXRF. The LOQ (10σ) was 0.4 mg/kg. The samples were also analyzed by inductively coupled plasma-optical emission spectrometry (ICP-OES) as an independent comparative method. Application of a paired t-test showed no significant differences between Zn concentrations obtained by EDXRF and ICP-OES (at a 95% confidence level)...
November 1, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28204789/potential-of-pelleted-wheat-straw-as-an-alternative-bedding-material-for-broilers
#16
S K Kheravii, R A Swick, M Choct, S-B Wu
No abstract text is available yet for this article.
February 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28203457/effect-of-strontium-on-nd-doped-ba1-x-sr-x-ce0-65zr0-25nd0-1o3-%C3%AE-proton-conductor-as-an-electrolyte-for-solid-oxide-fuel-cells
#17
J Madhuri Sailaja, K Vijaya Babu, N Murali, V Veeraiah
This paper investigated the Sr doping effect on the microstructure, chemical stability, and conductivity of Ba1-x Sr x Ce0.65Zr0.25Nd0.1O3-δ (0 ⩽ x ⩽ 0.2) electrolyte prepared by sol-gel method. The lattice constants and unit cell volumes were found to decrease as Sr atomic percentage increased in accordance with the Vegard law, confirming the formation of solid solution. Incorporation of Sr into the composition resulted in smaller grains besides suppressing the formation of secondary phases of SrCeO3...
May 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28202943/arterial-pressure-lability-is-improved-by-sodium-glucose-cotransporter-2-inhibitor-in-streptozotocin-induced-diabetic-rats
#18
Tomoko Yoshikawa, Takuya Kishi, Keisuke Shinohara, Ko Takesue, Risa Shibata, Noriyuki Sonoda, Toyoshi Inoguchi, Kenji Sunagawa, Hiroyuki Tsutsui, Yoshitaka Hirooka
To prevent cardiovascular events in patients with diabetes mellitus (DM), it is essential to reduce arterial pressure (AP). Sodium-glucose cotransporter 2 inhibitor (SGLT2i) prevents cardiovascular events via the depressor response in patients with DM. In the present study, we examined whether SGLT2i ameliorates AP lability in DM rats. Ten-week-old male Sprague-Dawley rats were administered a single intravenous injection of streptozotocin (50 mg kg(-1)) and were divided into three groups treated with low-dose SGLT2i, vehicle (VEH) or subcutaneously implanted insulin pellets (SGLT2i, VEH and Insulin group, respectively) for 14 days...
February 16, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28201935/partition-and-metabolic-fate-of-dietary-glycerol-in-muscles-and-liver-of-juvenile-tilapia
#19
Diego Vicente da Costa, Jorge Dias, Rita Colen, Priscila Vieira Rosa, Sofia Engrola
This study investigated the effect of dietary glycerol on the metabolism of juvenile tilapia (Oreochromis mossambicus) and to determine its metabolic fate. The experimental diets contained 0% (Group CON), 5% (Group G5) and 15% glycerol (Group G15) and were fed for 40 d to apparent satiation, three times a day. For the metabolism trials, six fish from each treatment were randomly chosen and tube-fed with five pellets labelled with (14)C-glycerol [(14)C(U)] in order to evaluate the absorption, catabolism, retention and partition of glycerol in muscle and liver...
April 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28199961/proteomic-analysis-of-laser-capture-microdissected-focal-lesions-in-a-rat-model-of-progenitor-marker-positive-hepatocellular-carcinoma
#20
Adeola O Adebayo Michael, Nagib Ahsan, Valerie Zabala, Heather Francois-Vaughan, Stephanie Post, Kate E Brilliant, Arthur R Salomon, Jennifer A Sanders, Philip A Gruppuso
We have shown previously that rapamycin, the canonical inhibitor of the mechanistic target of rapamycin (mTOR) complex 1, markedly inhibits the growth of focal lesions in the resistant hepatocyte (Solt-Farber) model of hepatocellular carcinoma (HCC) in the rat. In the present study, we characterized the proteome of persistent, pre-neoplastic focal lesions in this model. One group was administered rapamycin by subcutaneous pellet for 3 weeks following partial hepatectomy and euthanized 4 weeks after the cessation of rapamycin...
February 9, 2017: Oncotarget
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