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enterococcus faecalis biofilm formation

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https://www.readbyqxmd.com/read/28334141/distribution-of-antimicrobial-resistance-determinants-virulence-associated-factors-and-crispr-loci-in-isolates-of-enterococcus-faecalis-from-various-settings-and-genetic-lineages
#1
Iwona Gawryszewska, Katarzyna Malinowska, Alicja Kuch, Dorota Chrobak-Chmiel, Lucja Laniewska-Trokenheim, Waleria Hryniewicz, Ewa Sadowy
Enterococcus faecalis represents an important factor of hospital-associated infections (HAIs). The knowledge on its evolution from a commensal to an opportunistic pathogen is still limited, thus we performed a study to characterize distribution of factors that may contribute to this adaptation. Using a collection obtained from various settings (hospitalized patients, community carriers, animals, fresh food, sewage, water) we investigated differences in antimicrobial susceptibility, distribution of antimicrobial resistance genes, virulence-associated determinants and phenotypes, and CRISPR loci in the context of the clonal relatedness of isolates...
March 1, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28303818/biofilm-formation-capability-of-enterococcal-strains-causing-urinary-tract-infection-vis-a-vis-colonisation-and-correlation-with-enterococcal-surface-protein-gene
#2
Shubha Garg, Balvinder Mohan, Neelam Taneja
BACKGROUND: Data regarding differences in biofilm formation among urinary isolates of enterococci causing nosocomial infection versus asymptomatic colonisation is lacking. Conflicting data are available for the role of enterococcal surface protein (esp) gene in the development of enterococcal biofilms. MATERIALS AND METHODS: A total of 50 (25 each of asymptomatic bacteriuria and urinary tract infection (UTI) isolates were collected from admitted patients who had nosocomial acquisition of enterococci in urine culture (≥105 cfu/ml)...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28253588/-effects-of-epigallocatechin-3-gallate-modification-on-the-bonding-stability-of-an-etch-and-rinse-adhesive-to-intraradicular-dentin
#3
H H Yu, L Zhang, F Yu, H Zhou, L J Shen, J H Chen
Objective: To evaluate the effects of epigallocatechin-3-gallate (EGCG) modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin, and to find a new strategy to improve the stability of bonding interface. Methods: EGCG was incorporated into Single Bond 2 (SB2) with the concentration of 200 mg/L and 400 mg/L respectively to fabricated experimental adhesives group A and group B, while Single Bond 2 without EGCG was used as control group. Laser scanning confocal microscope (LSCM) and scanning electron microscope (SEM) were used to observe the bacterial biofilm adherent to the surface of the cured adhesive...
February 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/28242666/adjuvant-antibiotic-activity-of-acidic-sophorolipids-with-potential-for-facilitating-wound-healing
#4
Helen L Lydon, Niki Baccile, Breedge Callaghan, Roger Marchant, Christopher A Mitchell, Ibrahim M Banat
The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (>95% purity)...
February 27, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28237766/n-acetylcysteine-inhibits-growth-adhesion-and-biofilm-formation-of-gram-positive-skin-pathogens
#5
Daria Eroshenko, Tatyana Polyudova, Vladimir Korobov
N-Acetylcysteine (NAC) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of Gram-positive and Gram-negative bacteria. Our aim was to assess the effects of NAC on the growth of Gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. The minimum inhibitory concentrations (MICs) of NAC against Enterococcus faecalis, Corynebacterium ammoniagenes, Mycobacterium smegmatis, Propionibacterium acnes, Staphylococcus aureus, S. epidermidis, Streptococcus pyogenes, and 14 clinical strains of coagulase-negative staphylococci (CNS) ranged from 0...
February 22, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28194365/antibacterial-effect-of-urushiol-on-e-faecalis-as-a-root-canal-irrigant
#6
Sang-Wan Kim, Dong-Hoon Shin
OBJECTIVES: The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl. MATERIALS AND METHODS: The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at 37℃ for 7 days to allow biofilm formation...
February 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28151960/d-amino-acids-reduce-enterococcus-faecalis-biofilms-in-vitro-and-in-the-presence-of-antimicrobials-used-for-root-canal-treatment
#7
Peter S Zilm, Victor Butnejski, Giampiero Rossi-Fedele, Stephen P Kidd, Suzanne Edwards, Krasimir Vasilev
Enterococcus faecalis is the most frequent species present in post-treatment disease and plays a significant role in persistent periapical infections following root canal treatment. Its ability to persist in stressful environments is inter alia, due to its ability to form biofilms. The presence of certain D-amino acids (DAAs) has previously been shown to reduce formation of Bacillus subtilis biofilms. The aims of this investigation were to determine if DAAs disrupt biofilms in early and late growth stages for clinical E...
2017: PloS One
https://www.readbyqxmd.com/read/28108098/comparative-efficacies-of-daptomycin-vancomycin-and-linezolid-in-experimental-enterococcal-peritonitis
#8
Toshiki Kajihara, Shigeki Nakamura, Naoki Iwanaga, Kazuhiro Oshima, Katsuji Hirano, Taiga Miyazaki, Koichi Izumikawa, Katsunori Yanagihara, Yoshitsugu Miyazaki, Noboru Hattori, Nobuoki Kohno, Shigeru Kohno, Hiroshi Mukae
Enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. Although the in vitro susceptibility of daptomycin (DAP) against Enterococci is well established, the Food and Drug Administration has only approved its use for complicated skin and skin structure infections induced by Enterococcus faecalis. In this study we evaluated the potential therapeutic application of DAP in a murine model of enterococcal experimental peritonitis...
January 17, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28039075/comparative-genome-analysis-of-multiple-vancomycin-resistant-enterococcus-faecium-isolated-from-two-fatal-cases
#9
Shu Yong Lim, Kien-Pong Yap, Cindy Shuan Ju Teh, Kartini Abdul Jabar, Kwai Lin Thong
Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VREfm) strains isolated from two fatal cases in a tertiary hospital in Malaysia. Two sequence types, ST80 and ST203, were identified which belong to the clinically important clonal complex (CC) 17...
December 28, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27939738/effect-of-the-association-of-nonsteroidal-anti-inflammatory-and-antibiotic-drugs-on-antibiofilm-activity-and-ph-of-calcium-hydroxide-pastes
#10
Rafaela Pignatti de Freitas, Vanessa Raquel Greatti, Murilo Priori Alcalde, Bruno Cavalini Cavenago, Rodrigo Ricci Vivan, Marco Antonio Hungaro Duarte, Ana Carolina Villas Bôas Weckwerth, Paulo Henrique Weckwerth
INTRODUCTION: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs). METHODS: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication)...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27930849/searching-for-a-potential-antibacterial-lead-structure-against-bacterial-biofilms-among-new-naphthoquinone-compounds
#11
C S Moreira, A C J A Silva, J S Novais, A M Sá Figueiredo, V F Ferreira, D R da Rocha, H C Castro
AIMS: The aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against Gram-negative and Gram-positive bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, to probe for potential lead structures. METHODS AND RESULTS: Thirty-six new analogues were prepared with good yields using a simple, fast, operational three-procedure reaction and a thiol addition to an ο-quinone methide using microwave irradiation...
December 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27880799/treatment-of-oral-biofilms-by-a-d-enantiomeric-peptide
#12
Tian Zhang, Zhejun Wang, Robert E W Hancock, César de la Fuente-Núñez, Markus Haapasalo
Almost all dental diseases are caused by biofilms that consist of multispecies communities. DJK-5, which is a short D-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. Peptide DJK-5 at 10 μg/mL effectively prevented the growth of these microbes in culture media in a time-dependent manner. In addition to the prevention of growth, peptide DJK-5 completely killed both Streptococcus mutans and Enterococcus faecalis suspended from biofilms after 30 minutes of incubation in liquid culture media...
2016: PloS One
https://www.readbyqxmd.com/read/27878401/enterocin-b3a-b3b-produced-by-lab-collected-from-infant-faeces-potential-utilization-in-the-food-industry-for-listeria-monocytogenes-biofilm-management
#13
Alaa Al-Seraih, Yanath Belguesmia, John Baah, Sabine Szunerits, Rabah Boukherroub, Djamel Drider
Enterococcus faecalis B3A-B3B produces the bacteriocin B3A-B3B with activity against Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens, but apparently not against fungi or Gram-negative bacteria, except for Salmonella Newport. B3A-B3B enterocin has two different nucleotides but similar amino acid composition to the class IIb MR10A-MR10B enterocin. B3A-B3B consists of two peptides of predicted molecular mass of 5176.31 Da (B3A) and 5182...
February 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27812619/exopolysaccharide-dispelled-by-calcium-hydroxide-with-volatile-vehicles-related-to-bactericidal-effect-for-root-canal-medication
#14
Lei Lei, Meiying Shao, Yan Yang, Mengying Mao, Yingming Yang, Tao Hu
Objective: Enterococcus faecalis is the dominant microbial species responsible for persistent apical periodontitis with ability to deeply penetrate into the dentin. Exopolysaccharides (EPS) contribute to the pathogenicity and antibiotic resistance of E. faecalis. Our aim was to investigate the antimicrobial activity of calcium hydroxide (CH), camphorated parachlorophenol (CMCP), and chlorhexidine (CHX) against E. faecalis in dentinal tubules. Material and Methods: Decoronated single-canal human teeth and semicylindrical dentin blocks were incubated with E...
September 2016: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/27800142/antibacterial-efficacy-of-super-oxidized-water-on-enterococcus-faecalis-biofilms-in-root-canal
#15
Recai Zan, Tayfun Alacam, Ihsan Hubbezoglu, Tutku Tunc, Zeynep Sumer, Oguzhan Alici
BACKGROUND: The success of endodontic treatment depends on a few crucial factors. One of these factors is the complete chemomechanic preparation of root canal against various bacteria. In particular, the effect of resistant bacteria may cause intense pain with flare-up and formation of periapical lesions. Therefore, the strong effect of irrigants plays an important role in terms of the complete elimination of these bacteria to achieve long-term successful treatment. OBJECTIVES: The aim of this study was to investigate the antibacterial effects of super-oxidized water (SPO) in root canals infected with Enterococcus faecalis biofilms...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27770916/biofilm-behavior-on-sulfonated-poly-ether-ether-ketone-speek
#16
Juan F D Montero, Henrique A Tajiri, Guilherme M O Barra, Márcio C Fredel, Cesar A M Benfatti, Ricardo S Magini, Andréa L Pimenta, Júlio C M Souza
Poly(ether-ether-ketone) (PEEK) has also shown to be very attractive for incorporating therapeutic compounds thanks to a sulfonation process which modifies the material structure resulting in a sulfonated-PEEK (sPEEK). Concerning biomedical applications, the objective of this work was to evaluate the influence of different sulfonation degree of sPEEK on the biofilm growth. PEEK samples were functionalized by using sulphuric acid (98%) and then dissolved into dimethyl-sulfoxide. A dip coating technique was used to synthesize sPEEK thin films...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27770039/a-critical-role-for-extracellular-dna-in-dental-plaque-formation
#17
N Rostami, R C Shields, S A Yassin, A R Hawkins, L Bowen, T L Luo, A H Rickard, R Holliday, P M Preshaw, N S Jakubovics
Extracellular DNA (eDNA) has been identified in the matrix of many different monospecies biofilms in vitro, including some of those produced by oral bacteria. In many cases, eDNA stabilizes the structure of monospecies biofilms. Here, the authors aimed to determine whether eDNA is an important component of natural, mixed-species oral biofilms, such as plaque on natural teeth or dental implants. To visualize eDNA in oral biofilms, approaches for fluorescently stained eDNA with either anti-DNA antibodies or an ultrasensitive cell-impermeant dye, YOYO-1, were first developed using Enterococcus faecalis, an organism that has previously been shown to produce extensive eDNA structures within biofilms...
October 21, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27736645/enterococcal-metabolite-cues-facilitate-interspecies-niche-modulation-and-polymicrobial-infection
#18
Damien Keogh, Wei Hong Tay, Yao Yong Ho, Jennifer L Dale, Siyi Chen, Shivshankar Umashankar, Rohan B H Williams, Swaine L Chen, Gary M Dunny, Kimberly A Kline
Enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. E. faecalis co-exists with Escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. During infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. In this study, we investigated the contribution of E. faecalis to mixed-species infection when iron availability is restricted...
October 12, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27736638/enterococcus-faecalis-e-%C3%A2-coli-s-siderophore-inducing-sidekick
#19
Elizabeth R Hughes, Sebastian E Winter
Many infectious diseases involve polymicrobial infections, which are characterized by synergistic interactions between different microorganisms colonizing a host. In this issue of Cell Host & Microbe, Keogh et al. (2016) show that Enterococcus faecalis promotes Escherichia coli biofilm formation in low-iron conditions, thus facilitating polymicrobial growth.
October 12, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27690474/-effect-of-lipophosphonoxins-on-inhibition-of-bacterial-colonization-of-bone-cements
#20
R Večeřová, K Bogdanová, D Rejman, J Gallo, M Kolář
OBJECTIVE: The study aimed at determining the ability of lipophosphonoxin DR5026 to inhibit the formation of bacterial biofilm on the bone cement surface and assessing potential development of bacterial resistance. MATERIAL AND METHODS: Bone cement (Hi-Fatigue Bone Cement 2x40, aap Biomaterials GmbH, Germany) was polymerized with lipophosphonoxin DR5026. Cement samples were cultured using bacterial suspension containing Staphylococcus epidermidis CCM7221 at an inoculum density of 106 CFU/mL...
2016: Epidemiologie, Mikrobiologie, Imunologie
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