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enterococcus faecalis biofilm formation

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https://www.readbyqxmd.com/read/29723434/prgb-promotes-aggregation-biofilm-formation-and-conjugation-through-dna-binding-and-compaction
#1
Andreas Schmitt, Kai Jiang, Martha I Camacho, Venkateswara Rao Jonna, Anders Hofer, Fredrik Westerlund, Peter J Christie, Ronnie P-A Berntsson
Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer...
May 3, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29709604/ex-vivo-assessment-of-synergic-effect-of-chlorhexidine-for-enhancing-antimicrobial-photodynamic-therapy-efficiency-on-expression-patterns-of-biofilm-associated-genes-of-enterococcus-faecalis
#2
Behnam Bolhari, Maryam Pourhajibagher, Farzaneh Bazarjani, Nasim Chiniforush, Mehdi Rostami Rad, Salma Pirmoazen, Abbas Bahador
BACKGROUND: It has clearly demonstrated that Enterococcus faecalis, as a persistent microorganism is the major agent in the etiopatogeny of endodontic infections. Recently, the limitations of conventional endodontic therapy have given rise to many attempts to introduce antimicrobial photodynamic therapy (aPDT) as an alternative treatment. The aim of this study was to analyze the ex vivo effect of aPDT in combination with 2.0 % chlorhexidine (CHX) as a conventional therapy on colony count and expression patterns of genes associated with biofilm formation of E...
April 27, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29625241/phenotype-and-genotype-alteration-during-adaptive-evolution-of-enterococcus-faecalis-to-antimicrobials
#3
Yao Sun, Hong Lu, Xiaoxiao Zhang, Qing Wu, Wenzi Bi, Haiyang Liu, Jianming Cao, Tieli Zhou
The current worldwide emergence of resistance to antibiotics in bacteria constitutes an important growing public health threat. The mechanisms of the emergence and dissemination of resistance remain to be elucidated. Adaptation laboratory evolution provide an approach to investigate the acquisition of de novo mutations that confers drug resistance. In our study, 3 Enterococcus faecalis clinical isolates and E. faecalis ATCC29212 were evolved resistant to 8 kinds of antimicrobials spanning five chemical classes, including ciprofloxacin, levofloxacin, gatifloxacin, penicillin, imipenem, vancomycin, chloramphenicol and gentamicin...
April 3, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29550501/antibacterial-naphthoquinone-derivatives-targeting-resistant-strain-gram-negative-bacteria-in-biofilms
#4
Juliana S Novais, Caroline S Moreira, Ana Carolina J A Silva, Raquel S Loureiro, Agnes Marie Sá Figueiredo, Vitor F Ferreira, Helena C Castro, David R da Rocha
The aims of this study were the planning, synthesis and in vitro evaluation of 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against Gram-negative and Gram-positive strains, searching for potential lead compounds against bacterial biofilm formation. A series of 12 new analogs of 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones were synthesized by adding a thiol and different substituents to a ο-quinone methide using microwave irradiation. The compounds were tested against Gram-positive (Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S...
March 14, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29517121/effects-of-three-%C3%AE-alkylidene-%C3%AE-lactams-on-the-formation-of-multispecies-biofilms
#5
Josiane de Almeida, Andrea L Pimenta, Ulisses A Pereira, Luiz C A Barbosa, Michel A Hoogenkamp, Suzette V van der Waal, Wim Crielaard, Wilson T Felippe
This study evaluated the inhibitory effects of lactams on Streptococcus mutans, Enterococcus faecalis, and Candida glabrata multispecies biofilm formation. γ-Alkylidene-γ-lactams 1, 2, and 3 [solubilized in 3.5% dimethyl sulfoxide (DMSO)] were tested. Glass coverslips were conditioned with either the lactams or 3.5% DMSO (control) for 1 h, inoculated with microbial cultures, and incubated for 48 h. To assess the effect of the lactams on biofilm formation, the following parameters were determined: the biofilm biomass (by both crystal violet staining and protein determination); the amount of insoluble polysaccharides of the extracellular matrix; and the number of viable and total cells [by both colony-forming unit counting and quantitative real-time PCR (qPCR)]...
June 2018: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29470048/designing-porphyrinic-covalent-organic-frameworks-for-the-photodynamic-inactivation-of-bacteria
#6
Jan Hynek, Jaroslav Zelenka, Jiří Rathouský, Pavel Kubát, Tomáš Ruml, Jan Demel, Kamil Lang
Microbial colonization of biomedical devices is a recognized complication contributing to healthcare-associated infections. One of the possible approaches to prevent surfaces from the biofilm formation is antimicrobial photodynamic inactivation based on the cytotoxic effect of singlet oxygen, O2 (1 Δg ), a short-lived, highly oxidative species, produced by energy transfer between excited photosensitizers and molecular oxygen. We synthesized porphyrin-based covalent organic frameworks (COFs) by Schiff-base chemistry...
March 14, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29426645/novel-endodontic-disinfection-approach-using-catalytic-nanoparticles
#7
Sarah Bukhari, Dongyeop Kim, Yuan Liu, Bekir Karabucak, Hyun Koo
INTRODUCTION: The aim of this study was to test a new disinfection technology using biomimetic iron oxide nanoparticles (IO-NPs) with peroxidaselike activity to enhance antibacterial activity on root canal surfaces and in dentinal tubules. METHODS: The canal surfaces and dentinal tubules of single-rooted intact extracted teeth were infected by growing Enterococcus faecalis biofilms for 3 weeks. The samples were divided into 6 treatment groups: (1) phosphate-buffered saline (PBS) (negative control), (2) 3% hydrogen peroxide (H2 O2 ) (test control), (3) IO-NPs (0...
May 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29424388/evaluation-antibacterial-and-antibiofilm-activity-of-the-antimicrobial-peptide-p34-against-staphylococcus-aureus-and-enterococcus-faecalis
#8
Géssica A Costa, Fernanda C P Rossatto, Aline W Medeiros, Ana Paula F Correa, Adriano Brandelli, Ana Paula G Frazzon, Amanda DE S DA Motta
The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods...
January 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29409364/graphene-onto-medical-grade-titanium-an-atom-thick-multimodal-coating-that-promotes-osteoblast-maturation-and-inhibits-biofilm-formation-from-distinct-species
#9
Nileshkumar Dubey, Kassapa Ellepola, Fanny E D Decroix, Julien L P Morin, A H Castro Neto, Chaminda J Seneviratne, Vinicius Rosa
The time needed for the osseointegration of titanium implants is deemed too long. Moreover, the bacterial colonization of their surfaces is a major cause of failure. Graphene can overcome these issues but its wet transfer onto substrates employs hazardous chemicals limiting the clinical applications. Alternatively, dry transfer technique has been developed, but the biological properties of this technique remain unexplored. Here, a dry transfer technique based on a hot-pressing method allowed to coat titanium substrates with high-quality graphene and coverage area >90% with a single transfer...
May 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29372297/genomic-and-functional-characterisation-of-two-enterococcus-strains-isolated-from-cotija-cheese-and-their-potential-role-in-ripening
#10
Myrna Olvera-García, Alejandro Sanchez-Flores, Maricarmen Quirasco Baruch
Enterococcus spp. are present in the native microbiota of many traditional fermented foods. Their ability to produce antibacterial compounds, mainly against Listeria monocytogenes, has raised interest recently. However, there is scarce information about their proteolytic and lipolytic potential, and their biotechnological application is currently limited because enterococcal strains have been related to nosocomial infections. In this work, next-generation sequencing and optimised bioinformatic pipelines were used to annotate the genomes of two Enterococcus strains-one E...
March 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29363364/insights-into-biological-activity-of-ureidoamides-with-primaquine-and-amino-acid-moieties
#11
Josipa Vlainić, Ivan Kosalec, Kristina Pavić, Dimitra Hadjipavlou-Litina, Eleni Pontiki, Branka Zorc
Primaquine (PQ) ureidoamides 5a-f were screened for antimicrobial, biofilm eradication and antioxidative activities. Susceptibility of the tested microbial species towards tested compounds showed species- and compound-dependent activity. N-(diphenylmethyl)-2-[({4-[(6-methoxyquinolin-8-yl)amino]pentyl}carbamoyl)amino]-4-methylpentanamide (5a) and 2-(4-chlorophenyl)-N-(diphenylmethyl)-2-[({4-[(6-methoxyquinolin-8-yl)amino]pentyl}carbamoyl)amino]acetamide (5d) showed antibacterial activity against S. aureus strains (MIC = 6...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29358339/quantitative-proteomics-of-strong-and-weak-biofilm-formers-of-enterococcus-faecalis-reveals-novel-regulators-of-biofilm-formation
#12
Tanujaa Suriyanarayanan, Lin Qingsong, Lim Teck Kwang, Lee Yew Mun, Chaminda Jayampath Seneviratne
Enterococcus faecalis is a bacterial pathogen associated with both endodontic and systemic infections. The biofilm formation ability of E. faecalis plays a key role in its virulence and drug resistance attributes. The formation of E. faecalis biofilms on implanted medical devices often results in treatment failure. In the present study, we report protein markers associated with the biofilm formation ability of E. faecalis using iTRAQ-based quantitative proteomics approach. In order to elucidate the biofilm-associated protein markers, we investigated the proteome of strong and weak biofilm-forming E...
April 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29355416/antimicrobial-activity-of-a-colloidal-agnp-suspension-demonstrated-in-vitro-against-monoculture-biofilms-toward-a-novel-tooth-disinfectant-for-treating-dental-caries
#13
D R Schwass, K M Lyons, R Love, G R Tompkins, C J Meledandri
A novel silver nanoparticle (AgNP) formulation was developed as a targeted application for the disinfection of carious dentine. Silver nitrate (AgNO3 ) was chemically reduced using sodium borohydrate (NaBH4 ) in the presence of sodium dodecyl sulfate (SDS) to form micelle aggregate structures containing monodisperse 6.7- to 9.2-nm stabilized AgNPs. AgNPs were characterized by measurement of electrical conductivity and dynamic light scattering, scanning electron microscopy, transmission electron microscopy, and inductively coupled plasma mass spectrometry...
February 2018: Advances in Dental Research
https://www.readbyqxmd.com/read/29354778/the-efficacy-of-gaseous-ozone-against-different-forms-of-candida-albicans
#14
M Zargaran, M Fatahinia, A Zarei Mahmoudabadi
Background and Purpose: Ozone is an inorganic molecule with effective antimicrobial properties. Clinical treatment of ozonated water was used for the elimination of Candida albicans , Enterococcus faecalis , endotoxins, and biofilms from root canals. In addition, its therapeutic effects for tinea pedis, ulcers, and leishmaniasis were investigated. The purpose of the present study was to evaluate the fungicidal effects of ozone on different forms of C. albicans . In addition, antifungal susceptibility profile of strains was assessed before and after exposure to ozone...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29325735/whole-transcriptome-analysis-reveals-potential-novel-mechanisms-of-low-level-linezolid-resistance-in-enterococcus-faecalis
#15
Ruoyi Hua, Yun Xia, Wenyao Wu, Jia Yan, Mi Yang
Linezolid is an oxazolidinone antibiotic commonly used to treat serious infections caused by vancomycin-resistant enterococcus. Recently, low-level linezolid resistant Enterococcus faecalis strains have emerged worldwide, but the resistant mechanisms remain undefined. Whole-transcriptome profiling was performed on an E. faecalis strain P10748 with low-level linezolid resistance in comparison with a linezolid-susceptible strain 3138 and the standard control strain ATCC29212. The functions of differentially expressed genes (DEGs) were predicted, with some DEGs potentially involved in drug resistance were validated by PCR and quantitative PCR (qPCR)...
March 20, 2018: Gene
https://www.readbyqxmd.com/read/29285081/relevance-of-the-clustered-regularly-interspaced-short-palindromic-repeats-of-enterococcus-faecalis-strains-isolated-from-retreatment-root-canals-on-periapical-lesions-resistance-to-irrigants-and-biofilms
#16
Zhongchun Tong, Yu Du, Junqi Ling, Lijia Huang, Jinglei Ma
A high prevalence of Enterococcus faecalis ( E. faecalis ) is observed in teeth with root canal treatment failures. Clustered regularly interspaced short palindromic repeats (CRISPR) are widely distributed in prokaryotes that have adaptive immune systems against mobile elements, including pathogenic genes. The present study investigated the relevance of the CRISPR in E. faecalis strains isolated from retreated root canals on biofilms, periapical lesions and drug resistance. A total of 20 E. faecalis strains were extracted from the root canals of teeth referred for root canal retreatment...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29280095/the-efficacy-of-tetrasodium-edta-on-biofilms
#17
S L Percival, A-M Salisbury
The aetiology of delayed wound healing characteristic of a chronic wound is relatively unknown but is thought to be due to a combination of the patient's underlying pathophysiology and external factors including infection and biofilm formation. The invasion of the wound by the hosts' resident microbiome and exogenous microorganisms can lead to biofilm formation. Biofilms have increased tolerance to antimicrobial interventions and constitute a concern to chronic wound healing. Consequently, anti-biofilm technologies with proven efficacy in areas outside of wound care need evaluation to determine whether their efficacy could be relevant to the control of biofilms in wounds...
December 27, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29225595/characteristics-of-and-virulence-factors-associated-with-biofilm-formation-in-clinical-enterococcus-faecalis-isolates-in-china
#18
Jin-Xin Zheng, Yang Wu, Zhi-Wei Lin, Zhang-Ya Pu, Wei-Ming Yao, Zhong Chen, Duo-Yun Li, Qi-Wen Deng, Di Qu, Zhi-Jian Yu
Enterococcus faecalis biofilm traits and distribution characteristics in China have not been clarified. This study aimed to determine the prevalence and characteristics of E. faecalis biofilm formation in a sample of clinical isolates and to explore the virulence factors associated with biofilm formation in those isolates. A total of 265 E. faecalis isolates were collected from patients in Shenzhen, China. Virulence genes were detected within the genomes of the microbes by polymerase chain reaction. The isolates were subjected to multilocus sequence typing (MLST) based on housekeeping genes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29215338/evaluation-of-antimicrobial-durability-and-anti-biofilm-effects-in-urinary-catheters-against-enterococcus-faecalis-clinical-isolates-and-reference-strains
#19
Didem Kart, Ayşe Semra Kustimur, Meral Sağıroğlu, Ayşe Kalkancı
BACKGROUND: Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial-coated or impregnated urinary catheters are seen as a possible way to prevent these infections. AIMS: To determine the biofilm-forming ability of 89 E. faecalis isolates from urinary tract infections and to compare several urinary catheters for antimicrobial durability and the inhibitory effects on biofilm formation of different laboratory strains and clinical isolates of E...
December 1, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/29208399/association-between-extracellular-material-and-biofilm-formation-in-response-to-sodium-hypochlorite-by-clinical-isolates-of-enterococcus-faecalis
#20
Austin Yoo, Giampiero Rossi-Fedele, Stephen P Kidd, Anthony H Rogers, Peter S Zilm
INTRODUCTION: Extracellular material (ECM) surrounding Enterococcus faecalis may play a role in increasing resistance to environmental stresses. Our aim was to determine ECM levels in response to subminimal inhibitory concentrations of sodium hypochlorite (sub-MIC/NaOCl) or anaerobic growth and determine the impact on biofilm development. METHODS: From 37 E. faecalis clinical strains, 19 were selected according to their biofilm-producing ability by using a crystal violet biofilm assay: 10 strong, 4 intermediate, and 5 non-biofilm producers...
February 2018: Journal of Endodontics
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