keyword
MENU ▼
Read by QxMD icon Read
search

CS protein

keyword
https://www.readbyqxmd.com/read/28088654/transcriptional-response-of-skeletal-muscle-to-a-low-protein-perinatal-diet-in-rat-offspring-at-different-ages-the-role-of-key-enzymes-of-glucose-fatty-acid-oxidation
#1
José Luiz de Brito Alves, Ana Elisa Toscano, João Henrique da Costa-Silva, Hubert Vidal, Carol Góis Leandro, Luciano Pirola
Skeletal muscle is a plastic tissue during development with distinctive acute and chronic response to maternal protein restriction. This study evaluated gene and protein expression of key-enzymes of glycolytic pathway (HK2, PFK, PDK4 and CS), and fatty acid oxidation (CPT1 and β-HAD) of two different types of skeletal muscle [soleus and extensor digitorium longus (EDL)] from offspring rats at 30 and 90 days of age, exposed to maternal isoenergetic low protein diet throughout gestation and lactation. Pups from dams fed 17% protein diet (n=5, normal protein, Np), and low protein pups from dams fed 8% casein diet (low protein, Lp, n=5) were evaluated...
December 15, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#2
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28087273/chitosan-cross-linked-with-poly-ethylene-glycol-dialdehyde-via-reductive-amination-as-effective-controlled-release-carriers-for-oral-protein-drug-delivery
#3
Zi-Wei Jing, Zhi-Wei Ma, Chen Li, Yi-Yang Jia, Min Luo, Xi-Xi Ma, Si-Yuan Zhou, Bang-Le Zhang
The covalently cross-linked chitosan-poly(ethylene glycol)1540 derivatives have been developed as a controlled release system with potential for the delivery of protein drug. The swelling characteristics of the hydrogels based on these derivatives as the function of different PEG content and the release profiles of a model protein (bovine serum albumin, BSA) from the hydrogels were evaluated in simulated gastric fluid with or without enzyme in order to simulate the gastrointestinal tract conditions. The derivatives cross-linked with difunctional PEG1540-dialdehyde via reductive amination can swell in alkaline pH and remain insoluble in acidic medium...
December 31, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28078525/chitosan-ceramide-coating-on-gold-nanorod-to-improve-its-physiological-stability-and-reduce-the-lipid-surface-related-toxicity
#4
Gantumur Battogtokh, Oyuntuya Gotov, Young Tag Ko
Gold nanoparticles are promising materials for many applications that include imaging, drug delivery, and photothermal therapy. However, AuNPs can be unstable and/or toxic. We purposed to improve the stability and reduce toxicity of gold nanorods (AuNR) upon coating with biocompatible polymer, chitosan-ceramide (CS-CE), without replacing the original layer, CTAB. CS-CE-coated AuNR was prepared by simple mixing for 24 h and purified by centrifugation. The coating was confirmed by UV-Vis absorption analysis and surface charge and size measurement...
January 11, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28074912/influences-of-calcium-silicate-on-chemical-forms-and-subcellular-distribution-of-cadmium-in-amaranthus-hypochondriacus-l
#5
Huanping Lu, Zhian Li, Jingtao Wu, Yong Shen, Yingwen Li, Bi Zou, Yetao Tang, Ping Zhuang
A pot experiment was conducted to investigate the effects of calcium silicate (CS) on the subcellular distribution and chemical forms of cadmium (Cd) in grain amaranths (Amaranthus hypochondriacus L. Cv. 'K112') grown in a Cd contaminated soil. Results showed that the dry weight and the photosynthetic pigments contents in grain amaranths increased significantly with the increasing doses of CS treatments, with the highest value found for the treatment of CS3 (1.65 g/kg). Compared with the control, application of CS4 (3...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28063255/targeting-of-the-fun30-nucleosome-remodeller-by-the-dpb11-scaffold-facilitates-cell-cycle-regulated-dna-end-resection
#6
Susanne Cs Bantele, Pedro Ferreira, Dalia Gritenaite, Dominik Boos, Boris Pfander
DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination. Resection is thus under cell cycle control, but additionally regulated by chromatin and nucleosome remodellers. Here we show that both layers of control converge in the regulation of resection by the evolutionarily conserved Fun30/SMARCAD1 remodeller. Yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DSB-localized scaffold proteins Dpb11 and TOPBP1, respectively...
January 7, 2017: ELife
https://www.readbyqxmd.com/read/28063246/exacerbation-caused-by-5-asa-intolerance-in-children-with-ulcerative-colitis
#7
Hirotaka Shimizu, Katsuhiro Arai, Julian Tang, Kenji Hosoi, Rie Funayama
BACKGROUND: 5-aminosalicylate (5-ASA) is widely used as the first-line drug for ulcerative colitis (UC). 5-ASA is mostly a safe and effective drug, but it can bring about exacerbation due to 5-ASA intolerance. 5-ASA intolerance can be confusing and it can mislead physicians to consider unnecessary treatment escalation plan including corticosteroid (CS), biologics, or even surgery. In spite of the clinical importance of 5-ASA intolerance, there have been few publications about its incidence, clinical features, and diagnosis...
January 7, 2017: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/28062227/interleukin-1%C3%AE-secreted-from-betanodavirus-infected-microglia-caused-the-death-of-neurons-in-giant-grouper-brains
#8
Yu-Hui Chiang, Yu-Chi Wu, Shau-Chi Chi
High interleukin (IL)-1β gene expression was observed in dead giant grouper brains after nervous necrosis virus (NNV) infection. To investigate the neuronal death caused by NNV infection, primary tissue culture of giant grouper brains (pGB) was performed. In NNV-infected pGB cells, the viral capsid protein was detected in both neurons and microglia; furthermore, microglial proliferation and neuronal death were observed. The culture supernatant (CS) of NNV-infected pGB cells contained IL-1β and tumor necrosis factor-α, which were mainly released from the microglia...
January 3, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28046184/nutritive-value-of-extruded-or-multi-enzyme-supplemented-cold-pressed-soybean-cake-for-pigs
#9
T A Woyengo, R Patterson, C L Levesque
The objectives were to determine the standardized ileal digestibility (SID) of AA and NE value of cold-pressed soybean cake (CP-SBC), and the effect of extrusion or adding multi-enzyme to CP-SBC diet for growing pigs. Eight ileal-cannulated pigs (initial BW = 79.7 ± 3.97 kg) were fed 4 diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets included a cornstarch-based diet with CP-SBC, extruded CP-SBC, and SBC plus multi-enzyme (1,200 U of xylanase, 150 U of glucanase, 500 U of cellulase, 60 U of mannanase, 700 U of invertase, 5,000 U of protease, and 12,000 U of amylase/kilogram of diet; Superzyme-CS, 0...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28038739/physicochemical-antioxidant-and-biocompatible-properties-of-chondroitin-sulphate-isolated-from-chicken-keel-bone-for-potential-biomedical-applications
#10
Aruna Rani, Rwivoo Baruah, Arun Goyal
Chicken keel bone cartilage was explored for cheaper and sustainable source for isolation of chondroitin sulphate (CS) for its future use in tissue engineering and pharmaceutical industry. HPSEC analysis displayed two peaks of 100kDa for CS-keel polysaccharide and 1kDa for protein. DLS analysis of CS-keel displayed polydispersity. CS-keel yield was 15% and 53±5% uronic acid content. The quantified percentages of UA-GalNAc4S and UA-GalNAc6S disaccharide in CS-keel were 58% and 42%, respectively. FT-IR identified CS-keel to be chondroitin 4-sulphate...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28036086/formation-and-physiochemical-properties-of-silver-nanoparticles-with-various-exopolysaccharides-of-a-medicinal-fungus-in-aqueous-solution
#11
Wenjie Jian, Lu Zhang, Ka-Chai Siu, Angxin Song, Jian-Yong Wu
Natural polysaccharides are the most widely used biopolymers for green synthesis of eco-friendly silver nanoparticles (AgNPs). In a previous study, a high molecular weight (MW) fraction of exopolysaccharides (EPS) produced by a medicinal fungus Cs-HK1 has been shown useful for green and facile synthesis of AgNPs in water. This study was to further evaluate the effects of molecular properties of EPS on the formation, stability and properties of AgNPs with different EPS fractions at various pH conditions. Three EPS fractions (P0...
December 29, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28028723/shenfu-injection-inhibits-inflammation-in-patients-with-acute-myocardial-infarction-complicated-by-cardiac-shock
#12
Yan-Yan Jin, Hai Gao, Xin-Yong Zhang, Hui Ai, Xiao-Lin Zhu, Jing Wang
OBJECTIVES: To investigate the effect of Shenfu Injection (, SFI) on inflammatory factors in patients with acute myocardial infarction complicated by cardiogenic shock (CS) treated with and intra-aortic balloon pump (IABP). METHODS: This study enrolled 60 patients with ST-segment elevation myocardial infarction (STEMI) complicated by CS. Patients underwent IABP and emergency percutaneous coronary intervention (PCI) were randomly divided into two groups by random number table with 30 cases in each group, one given SFI treatment (100 mL/24 h), one not...
December 27, 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28026993/connective-tissue-growth-factor-promotes-pulmonary-epithelial-cell-senescence-and-is-associated-with-copd-severity
#13
Jun-Ho Jang, Hitendra S Chand, Shannon Bruse, Melanie Doyle-Eisele, Christopher Royer, Jacob McDonald, Clifford Qualls, Aloysius J Klingelhutz, Yong Lin, Rama Mallampalli, Yohannes Tesfaigzi, Toru Nyunoya
The purpose of this study was to determine whether expression of connective tissue growth factor (CTGF) protein in chronic obstructive pulmonary disease (COPD) is consistent in humans and animal models of COPD and to investigate the role of this protein in lung epithelial cells. CTGF in lung epithelial cells of ex-smokers with COPD was compared with ex-smokers without COPD by immunofluorescence. A total of twenty C57Bl/6 mice and sixteen non-human primates (NHPs) were exposed to cigarette smoke (CS) for 4 weeks...
December 27, 2016: COPD
https://www.readbyqxmd.com/read/28024754/mips-graphene-nanoplatelets-mwcnts-modified-glassy-carbon-electrode-for-the-determination-of-cardiac-troponin-i
#14
Ya Ma, Xiao-Lei Shen, Hai-Shui Wang, Jia Tao, Jian-Zhi Huang, Qiang Zeng, Li-Shi Wang
An electrochemical sensor with high selectivity in addition to sensitivity was developed for the determination of cardiac troponin I (cTnI), based on the modification of cTnI imprinted polymer film on a glassy carbon electrode (GCE). The sensor was fabricated by layer-by-layer assembled graphene nanoplatelets (GS), multiwalled carbon nanotubes (MWCNTs), chitosan (CS), glutaraldehyde (GA) composites, which can increase the electronic transfer rate and the active surface area to capture a larger number of antigenic proteins...
December 23, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/28024612/fabrication-of-a-three-dimensional-%C3%AE-tricalcium-phosphate-gelatin-containing-chitosan-based-nanoparticles-for-sustained-release-of-bone-morphogenetic-protein-2-implication-for-bone-tissue-engineering
#15
Farshid Bastami, Zahrasadat Paknejad, Maissa Jafari, Majid Salehi, Maryam Rezai Rad, Arash Khojasteh
Fabrication of an ideal scaffold having proper composition, physical structure and able to have sustained release of growth factors still is challenging for bone tissue engineering. Current study aimed to design an appropriate three-dimensional (3-D) scaffold with suitable physical characteristics, including proper compressive strength, degradation rate, porosity, and able to sustained release of bone morphogenetic protein-2 (BMP2), for bone tissue engineering. A highly porous 3-D β-tricalcium phosphate (β-TCP) scaffolds, inside of which two perpendicular canals were created, was fabricated using foam-casting technique...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28012925/cigarette-smoke-extract-induces-epithelial-mesenchymal-transition-of-human-bladder-cancer-t24-cells-through-activation-of-erk1-2-pathway
#16
Xin Sun, Qifei Deng, Zhaofeng Liang, Zhiqi Liu, Hao Geng, Li Zhao, Qirui Zhou, Jie Liu, Jiaxing Ma, Daming Wang, Dexin Yu, Caiyun Zhong
Bladder cancer is a common genitourinary malignant disease worldwide. Abundant evidence has shown that cigarette smoke (CS) is a crucial risk factor for bladder cancer. Nevertheless, the mechanism underlying the relationship between cigarette smoking and bladder cancer remains unclear. In the present study, we investigated the effects of cigarette smoke extract (CSE) on mitogen-activated protein kinase (MAPK) pathway activation and EMT alterations in human bladder cancer T24 cells, and the preventive effect of extracellular regulated protein kinases 1 and 2 (ERK1/2) inhibitor U0126 was further examined...
December 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28012591/facile-preparation-of-polysaccharide-functionalized-macroporous-adsorption-resin-for-highly-selective-enrichment-of-glycopeptides
#17
Xudong Sun, Jing Dong, Jinan Li, Mingliang Ye, Weibing Zhang, Junjie Ou
A facile approach for preparation of hydrophilic polysaccharide-functionalized macroporous adsorption resin (MAR) was presented. Polydisperse MAR with approximately 250μm in diameter was synthesized via suspension polymerization and then modified with polysaccharides, sodium hyaluronate (HA) and chitosan (CS), through layer-by-layer (LbL) self-assembly process. The preparation method was facile, low cost and easy to expand production from tens of grams to one kilogram. The resulting MAR@(HA/CS)20 possesses highly hydrophilic property and rapid adsorption behavior...
December 16, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28008246/the-administration-of-a-high-refined-carbohydrate-diet-promoted-an-increase-in-pulmonary-inflammation-and-oxidative-stress-in-mice-exposed-to-cigarette-smoke
#18
Karina Braga Pena, Camila de Oliveira Ramos, Nícia Pedreira Soares, Pamela Félix da Silva, Ana Carla Balthar Bandeira, Guilherme de Paula Costa, Sílvia Dantas Cangussú, André Talvani, Frank Silva Bezerra
This study aimed to evaluate the effects of a high refined carbohydrate diet and pulmonary inflammatory response in C57BL/6 mice exposed to cigarette smoke (CS). Twenty-four male mice were divided into four groups: control group (CG), which received a standard diet; cigarette smoke group (CSG), which was exposed to CS; a high refined carbohydrate diet group (RG), which received a high refined carbohydrate diet; and a high refined carbohydrates diet and cigarette smoke group (RCSG), which received a high refined carbohydrate diet and was exposed to CS...
2016: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28006950/augmentation-of-s-nitrosoglutathione-gsno-controls-cigarette-smoke-induced-inflammatory-oxidative-stress-and-copd-emphysema-pathogenesis-by-restoring-cftr-function
#19
Manish Bodas, David Silverberg, Kyla Walworth, Kathryn A Brucia, Neeraj Vij
AIMS: Cigarette-smoke (CS) mediated CFTR-dysfunction, autophagy-impairment, and resulting inflammatory-oxidative/nitrosative stress leads to COPD-emphysema pathogenesis. Nitric oxide (NO)-signaling regulates lung function, and low serum NO-levels correlate with COPD severity. We aim to evaluate here the effects and mechanism(s) of GSNO-augmentation in COPD-emphysema pathogenesis. RESULTS: Our data shows that CFTR co-localizes with aggresome-bodies in the lungs of COPD subjects with increasing emphysema severity (GOLD I-IV) as compared to non-emphysema controls (GOLD-0)...
December 22, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/27993784/hum-mploc-3-0-prediction-enhancement-of-human-protein-subcellular-localization-through-modeling-the-hidden-correlations-of-gene-ontology-and-functional-domain-features
#20
Hang Zhou, Yang Yang, Hong-Bin Shen
MOTIVATION: Protein subcellular localization prediction has been an important research topic in computational biology over the last decade. Various automatic methods have been proposed to predict locations for large scale protein datasets, where statistical machine learning algorithms are widely used for model construction. A key step in these predictors is encoding the amino acid sequences into feature vectors. Many studies have shown that features extracted from biological domains, such as gene ontology and functional domains, can be very useful for improving the prediction accuracy...
December 19, 2016: Bioinformatics
keyword
keyword
81225
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"