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trichostatin a

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https://www.readbyqxmd.com/read/29157123/trichostatin-a-induces-nag-1-expression-and-apoptosis-in-human-endometriotic-stromal-cells
#1
Seok Kyo Seo, Jae Hoon Lee, Seung Joo Chon, Bo Hyon Yun, Sihyun Cho, Young Sik Choi, Byung Seok Lee
To investigate the effects of trichostatin A (TSA) on nonsteroidal anti-inflammatory drug-activated gene 1 (NAG-1) expression and apoptosis in human endometriotic stromal cells (HESCs), ectopic endometrial tissues were obtained from 15 patients with endometriotic cysts who underwent cystectomy. Human endometriotic stromal cells were isolated and cultured with different concentrations of TSA. Nonsteroidal anti-inflammatory drug-activated gene-1 messenger RNA (mRNA) and protein levels were evaluated by real-time polymerase chain reaction and Western blotting, respectively, and apoptosis was assessed by flow cytometry...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29151363/functional-assessment-of-human-enhancer-activities-using-whole-genome-starr-sequencing
#2
Yuwen Liu, Shan Yu, Vineet K Dhiman, Tonya Brunetti, Heather Eckart, Kevin P White
BACKGROUND: Genome-wide quantification of enhancer activity in the human genome has proven to be a challenging problem. Recent efforts have led to the development of powerful tools for enhancer quantification. However, because of genome size and complexity, these tools have yet to be applied to the whole human genome. RESULTS:  In the current study, we use a human prostate cancer cell line, LNCaP as a model to perform whole human genome STARR-seq (WHG-STARR-seq) to reliably obtain an assessment of enhancer activity...
November 20, 2017: Genome Biology
https://www.readbyqxmd.com/read/29142427/cinnamaldehyde-cinnamic-acid-and-cinnamyl-alcohol-the-bioactives-of-cinnamomum-cassia-exhibit-hdac8-inhibitory-activity-an-in-vitro-and-in-silico-study
#3
Mangesh Patil, Amit S Choudhari, Savita Pandita, Md Ataul Islam, Prerna Raina, Ruchika Kaul-Ghanekar
Background: The altered expression of histone deacetylase family member 8 (HDAC8) has been found to be linked with various cancers, thereby making its selective inhibition a potential strategy in cancer therapy. Recently, plant secondary metabolites, particularly phenolic compounds, have been shown to possess HDAC inhibitory activity. Objective: In the present work, we have evaluated the potential of cinnamaldehyde (CAL), cinnamic acid (CA), and cinnamyl alcohol (CALC) (bioactives of Cinnamomum) as well as aqueous cinnamon extract (ACE), to inhibit HDAC8 activity in vitro and in silico...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29111742/increased-post-translational-lysine-acetylation-of-myelin-basic-protein-is-associated-with-peak-neurological-disability-in-a-mouse-experimental-autoimmune-encephalomyelitis-model-of-multiple-sclerosis
#4
Ryan Lillico, Ting Zhou, Tina Khorshid Ahmad, Nicholas Stesco, Kiana Gozda, Jessica Truong, Jiming Kong, Ted M Lakowski, Michael Namaka
Citrullination of arginine residues is a post-translational modification (PTM) found on myelin basic protein (MBP), which neutralizes MBPs positive charge, and is implicated in myelin damage and multiple sclerosis (MS). Here we identify lysine acetylation as another neutralizing PTM to MBP that may be involved in myelin damage. We quantify changes in lysine and arginine PTMs on MBP derived from mice induced with an experimental autoimmune encephalomyelitis (EAE) model of MS using liquid chromatography tandem mass spectrometry...
November 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29107335/analysis-of-chromatin-opening-in-heterochromatic-non-small-cell-lung-cancer-tumor-initiating-cells-in-relation-to-dna-damaging-antitumor-treatment
#5
Mina Eriksson, Petra Hååg, Beata Brzozowska, Magdalena Lipka, Halina Lisowska, Rolf Lewensohn, Andrzej Wojcik, Kristina Viktorsson, Lovisa Lundholm
PURPOSE: We previously reported that sphere-forming non-small cell lung cancer (NSCLC) tumor-initiating cells (TICs) have an altered activation of DNA damage response- and repair proteins and are refractory to DNA-damaging treatments. We analyzed whether chromatin organization plays a role in the observed refractoriness. METHODS AND MATERIALS: Bulk cells and TICs from the NSCLC H23 and H1299 cell lines were examined using cell viability, clonogenic survival, Western blot, short interfering RNA analysis, and micronucleus assay...
September 22, 2017: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/29101337/trichostatin-a-ameliorates-renal-tubulointerstitial-fibrosis-through-modulation-of-the-jnk-dependent-notch-2-signaling-pathway
#6
Chun-Wu Tung, Yung-Chien Hsu, Chang-Jhih Cai, Ya-Hsueh Shih, Ching-Jen Wang, Pey-Jium Chang, Chun-Liang Lin
Renal fibrosis is the final common pathological feature in a variety of chronic kidney disease. Trichostatin A (TSA), a histone deacetylase inhibitor, reportedly attenuates renal fibrosis in various kidney disease models. However, the detailed molecular action of TSA in ameliorating renal fibrotic injury is not yet fully understood. In a cultured renal fibroblastic cell model, we showed that TGF-β1 triggers upregulation of α-SMA and fibronectin, two hallmarks of myofibroblastic activation. During the course of TGF-β1 treatment, activation of Smad2/3, p38, ERK, JNK and Notch-2 was also detected...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100282/up-regulation-of-irf-3-expression-through-gata-1-acetylation-by-histone-deacetylase-inhibitor-in-lung-adenocarcinoma-a549-cells
#7
Lu-Lu Wang, Lan-Bo Zhou, Jin Shu, Nan-Nan Li, Hui-Wen Zhang, Rui Jin, Li-Li Zhuang, Guo-Ping Zhou
Interferon regulatory factor 3 (IRF-3) is an important transcription factor for interferon genes. Although its functional activation by viral infection has been widely explicated, the regulatory mechanism of IRF-3 gene expression in cancer cells is poorly understood. In this study, we demonstrated treatment of lung adenocarcinoma A549 cells with trichostatin A (TSA) and valproic acid (VPA), two different classes of histone deacetylase inhibitors, strongly stimulated IRF-3 gene expression. Truncated and mutated IRF-3 promoter indicated that a specific GATA-1 element was responsible for TSA-induced activation of IRF-3 promoter...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29099289/trichostatin-a-decreases-the-levels-of-mecp2-expression-and-phosphorylation-and-increases-its-chromatin-binding-affinity
#8
Katrina V Good, Alexia Martínez de Paz, Monica Tyagi, Manjinder S Cheema, Anita A Thambirajah, Taylor L Gretzinger, Gilda Stefanelli, Bob Chow, Oliver Krupke, Michael Hendzel, Kristal Missiaen, Alan Underhill, Nicoletta Landsberger, Juan Ausió
MeCP2 binds to methylated DNA in a chromatin context and has an important role in cancer and brain development and function. Histone deacetylase (HDAC) inhibitors are currently being used to palliate many cancer and neurological disorders. Yet, the molecular mechanisms involved are not well known for the most part and, in particular, the relationship between histone acetylation and MeCP2 is not well understood. In this paper, we study the effect of the HDAC inhibitor trichostatin A (TSA) on MeCP2, a protein whose dysregulation plays an important role in these diseases...
November 3, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29095525/trichostatin-a-alters-cytoskeleton-and-energy-metabolism-of-pancreatic-adenocarcinoma-cells-an-in-depth-proteomic-study
#9
Elisa Dalla Pozza, Marcello Manfredi, Jessica Brandi, Arianna Buzzi, Eleonora Conte, Raffaella Pacchiana, Daniela Cecconi, Emilio Marengo, Massimo Donadelli
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal of all human cancers with a high mortality rate. Resistance to conventional treatments and chemotherapeutics is a typical feature of PDAC. To investigate the causes of drug resistance it is essential to deeply investigate the mechanism of action of chemotherapeutics. In this study, we performed an in depth shotgun proteomic approach using the label-free proteomic SWATH-MS analysis to investigate novel insights of the mechanism of action of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in PDAC cells...
November 2, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29075115/novel-biomolecule-lycopene-reduced-graphene-oxide-silver-nanoparticle-enhances-apoptotic-potential-of-trichostatin-a-in-human-ovarian-cancer-cells-skov3
#10
Xi-Feng Zhang, Feng-Hua Huang, Guo-Liang Zhang, Ding-Ping Bai, De Felici Massimo, Yi-Fan Huang, Sangiliyandi Gurunathan
BACKGROUND: Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29072686/suppression-of-bmp-7-by-histone-deacetylase-2-promoted-apoptosis-of-renal-tubular-epithelial-cells-in-acute-kidney-injury
#11
Taotao Ma, Cheng Huang, Qingqing Xu, Yang Yang, Yaru Liu, Xiaoming Meng, Jun Li, Min Ye, Hong Liang
Cisplatin, a highly effective and widely used chemotherapeutic agent, has a major limitation for its nephrotoxicity. Currently, there are no therapies available to treat or prevent cisplatin nephrotoxicity. We recently identified a novel strategy for attenuating its nephrotoxicity in chemotherapy by histone deacetylase (HDAC) inhibitors via epigenetic modification to enhance bone morphogenetic protein 7 (BMP-7) expression. Cisplatin upregulated the activity of HDAC2 in the kidney. Inhibition of HDAC with clinically used trichostatin A (TSA) or valproic acid (VPA) suppressed cisplatin-induced kidney injury and epithelial cell apoptosis...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29064603/histone-deacetylase-inhibitor-stimulates-e2-and-p4-secretion-in-sika-deer-ovarian-granulosa-cells-at-a-moderate-dose
#12
Mingjie Xing, Xiumin Chen, Xiaoxia Li, Yifeng Yang, Xiaoxu Wang, Xinyan Cao, Hailong Xue, Shiyong Wang, Yunfei Diao, Weigang Zhao, Meng Zhao, Xuezhe Cui, Tong Chang, Baozeng Xu, Haijun Wei
The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29061669/lysine-acetylome-profiling-uncovers-novel-histone-deacetylase-substrate-proteins-in-arabidopsis
#13
Markus Hartl, Magdalena Füßl, Paul J Boersema, Jan-Oliver Jost, Katharina Kramer, Ahmet Bakirbas, Julia Sindlinger, Magdalena Plöchinger, Dario Leister, Glen Uhrig, Greg Bg Moorhead, Jürgen Cox, Michael E Salvucci, Dirk Schwarzer, Matthias Mann, Iris Finkemeier
Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcellular compartments. In this study, we determined the proteome-wide signatures of the RPD3/HDA1 class of histone deacetylases in Arabidopsis Relative quantification of the changes in the lysine acetylation levels was determined on a proteome-wide scale after treatment of Arabidopsis leaves with deacetylase inhibitors apicidin and trichostatin A...
October 23, 2017: Molecular Systems Biology
https://www.readbyqxmd.com/read/29054681/role-of-histone-acetylation-in-activation-of-nuclear-factor-erythroid-2-related-factor-2-heme-oxygenase-1-pathway-by-manganese-chloride
#14
Zhipeng Zhang, Zhenkun Guo, Yanting Zhan, Huangyuan Li, Siying Wu
Manganese neurotoxicity is characterized by Parkinson-like symptoms with degeneration of dopaminergic neurons in the basal ganglia as the principal pathological feature. Manganese neurotoxicity studies may contribute to a good understanding of the mechanism of Parkinson's disease (PD). In this study, we first confirmed that MnCl2 can promote the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) protein in the nucleus or cytoplasm while increasing the binding activity of Nrf2 and antioxidant response elements, further promoting the expression of downstream target gene heme oxygenase 1 (HO-1) and leading to increase levels of reactive oxygen species (ROS) and reduce the levels of reduced glutathione (GSH)...
October 17, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29052293/butyrate-promotes-visceral-hypersensitivity-in-an-ibs-like-model-via-enteric-glial-cell-derived-nerve-growth-factor
#15
X Long, M Li, L-X Li, Y-Y Sun, W-X Zhang, D-Y Zhao, Y-Q Li
BACKGROUND: Altered visceral sensation is common in irritable bowel syndrome (IBS) and nerve growth factor (NGF) participates in visceral pain development. Sodium butyrate (NaB) could induce colonic hypersensitivity via peripheral up-regulation of NGF in animals. Enteric glial cells (EGCs) appear to be an important source of NGF. Whether butyrate could induce visceral hypersensitivity via increased EGC-derived NGF is still unknown. METHODS: CRL-2690 cells were used for transcriptome analyses after butyrate treatment...
October 20, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29039546/antiproliferative-effects-of-tsa-pxd%C3%A2-101-and-ms%C3%A2-275-in-a2780-and-mcf7-cells-acetylated-histone-h4-and-acetylated-tubulin-as-markers-for-hdaci-potency-and-selectivity
#16
Vasilis P Androutsopoulos, Demetrios A Spandidos
Inhibition of histone deacetylase enzymes (HDACs) has been well documented as an attractive target for the development of chemotherapeutic drugs. The present study investigated the effects of two prototype hydroxamic acid HDAC inhibitors, namely Trichostatin A (TSA) and Belinostat (PXD‑101) and the benzamide Entinostat (MS‑275) in A2780 ovarian carcinoma and MCF7 breast adenocarcinoma cells. The three HDACi inhibited the proliferation of A2780 and MCF7 cells at comparable levels, below the µM range. Enzyme inhibition assays in a cell‑free system showed that TSA was the most potent inhibitor of total HDAC enzyme activity followed by PXD‑101 and MS‑275...
October 6, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29027833/trichostatin-a-and-5-aza-2-deoxycytidine-influence-the-expression-of-cold-induced-genes-in-arabidopsis
#17
Yuan Song, Lijun Liu, Gaopeng Li, Lizhe An, Lining Tian
The expression of cold-induced genes is critical for plants to survive under freezing stress. However, the underlying mechanisms for the decision of when, where, and which genes to express are unclear when a plant meets a sudden temperature drop. Previous studies have demonstrated epigenetics to play a central role in the regulation of gene expression in plant responses to environmental stress. DNA methylation and histone deacetylation are the two most important epigenetic modifications. This study was conducted to investigate the effects of inhibiting DNA methylation and histone deacetylation on gene expression, and to explore the potential role of epigenetics in plant responses to cold stress...
October 13, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29019029/hippocampal-acetylation-may-improve-prenatal-stress-induced-depression-like-behavior-of-male-offspring-rats-through-regulating-ampars-expression
#18
Yong Lu, Junli Zhang, Lin Zhang, Shaokang Dang, Qian Su, Huiping Zhang, Tianwei Lin, Xiaoxiao Zhang, Yurong Zhang, Hongli Sun, Zhongliang Zhu, Hui Li
This study is to determine the role and mechanism of hippocampal acetylation in prenatal stress (PS) induced depression-like behavior of male offspring rats. PS-induced depression rat model was established. Sucrose preference and forced swim test were used to observe the behavior changes of male offspring rats. Hippocampal acetylation was induced by Trichostatin A injection. Quantitative real-time PCR and Western blot were used to determine the changes of AMPARs in acetylated hippocampus. The behavioral tests proved that AMPA was involved in the PS-induced depression-like behavior in offspring rats...
December 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28993733/lncrna-uc002mbe-2-interacting-with-hnrnpa2b1-mediates-akt-deactivation-and-p21-up-regulation-induced-by-trichostatin-in-liver-cancer-cells
#19
Ting Chen, Chengxin Gu, Cailin Xue, Tao Yang, Yun Zhong, Shiming Liu, Yuqiang Nie, Hui Yang
Long non-coding RNAs (lncRNAs) have been implicated in liver carcinogenesis. We previously showed that the induction of lncRNA-uc002mbe.2 is positively associated with the apoptotic effect of trichostatin A (TSA) in hepatocellular carcinoma (HCC) cells. The current study further analyzed the role of uc002mbe.2 in TSA-induced liver cancer cell death. The level of uc002mbe.2 was markedly increased by TSA in the cytoplasm of HCC cells. Knockdown of uc002mbe.2 prohibited TSA-induced G2/M cell cycle arrest, p21 induction, and apoptosis of Huh7 cells and reversed the TSA-mediated decrease in p-AKT...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28993195/effect-of-trichostatin-a-on-radiation-induced-epithelial-mesenchymal-transition-in-a549%C3%A2-cells
#20
SunilGowda Sunnaghatta Nagaraja, Vishnuvarthan Krishnamoorthy, Raghavi Raviraj, Alagudinesh Paramasivam, Devipriya Nagarajan
Radiotherapy is used to treat tumors of different origins and nature, but often lead to development of radioresistance and metastasis of cells. Interestingly, radiation induces epithelial-mesenchymal transition (EMT), a process by which epithelial cells undergo mesenchymal phenotype and stimulates tumor progression capability. Our study investigated the effect of Trichostatin A (TSA), a natural derivate isolated from Streptomyces, upon radiation-induced lung EMT and we tried to understand the role of signaling molecules in irradiated lung cancer cells (A549)...
December 2, 2017: Biochemical and Biophysical Research Communications
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