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Kyung Sook Kim, Ting Ting Zhao, Keon Sung Shin, Hyun Jin Park, Yoon Jeong Cho, Kyung Eun Lee, Seung Hwan Kim, Myung Koo Lee
This study investigated the effects of ethanol extract from Gynostemma pentaphyllum (GP-EX) on memory deficits in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned mouse model of Parkinson's disease (PD) (MPTP-lesioned mice). MPTP (30 mg/kg/day, 5 days)-lesioned mice showed deficits of habit learning memory and spatial memory, which were further aggravated by treatment with L-3,4-dihydroxyphenylalanine (L-DOPA) (25 mg/kg, 21 days). However, treatment with GP-EX (50 mg/kg, 21 days) ameliorated memory deficits in MPTP-lesioned mice treated with L-DOPA (25 mg/kg): GP-EX prevented the decreases in retention latency time in the passive avoidance test and tyrosine hydroxylase-immunopositive cells and dopamine levels in the nigrostriatum...
January 2017: Journal of Medicinal Food
Wenhui Jiang, Hu Shan, Jiying Song, Haitao Lü
A rapid and efficient method for the separation and purification of ombuoside from Gynostemma pentaphyllum by microwave-assisted extraction coupled with high-speed counter-current chromatography (HSCCC) was successfully developed. Using an orthogonal array design L9 (3(4)), the extraction conditions, including microwave power, irradiation time, solid-to-liquid ratio and extraction times, were optimized. Ombuoside was isolated and purified from the crude extraction by HSCCC with two-phase solvent system composed of n-hexane:ethyl acetate:ethanol:water (5:6:5:5, v/v) in a single run...
January 2017: Journal of Chromatographic Science
D Leach, I Ahmed, J De Voss, H Wohlmuth
No abstract text is available yet for this article.
December 2016: Planta Medica
Voravuth Somsak, Preeyanuch Borkaew, Chokdee Klubsri, Kittiyaporn Dondee, Panatda Bootprom, Butsarat Saiphet
Due to the emergence and spread of malaria parasite with resistance to antimalarial drugs, discovery and development of new, safe, and affordable antimalarial are urgently needed. In this respect, medicinal plant extracts are targets to optimize antimalarial actions and restore efficacy of standard antimalarial drugs. The present study was aimed at determining the antimalarial activities of Gynostemma pentaphyllum and Moringa oleifera leaf extracts in combination with artesunate against Plasmodium berghei-infected mice...
2016: Journal of Tropical Medicine
Song Guo, Chengxu Sui, Ying Ma
A sensitive, selective and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of gypenoside XLIX, a naturally occurring gypenoside of Gynostemma pentaphyllum in rat plasma and then validated according to the US Food and Drug Administration's Guidance for Industry: Bioanalytical Method Validation. Plasma samples were prepared by a simple solid-phase extraction. Separation was performed on a Waters XBridgeTM BEH C18 chromatography column (4.6 × 50 mm, 2...
November 16, 2016: Biomedical Chromatography: BMC
Huihong Guo, Rufang Li, Shibiao Liu, Na Zhao, Shuo Han, Mengmeng Lu, Xiaomin Liu, Xinli Xia
Gynostemma pentaphyllum (Thunb.) Makino is a perennial medicinal herb widely distributed in China. This herb contains important medicinal components called gypenosides, which belong to dammarane-type triterpenoid saponins. Squalene epoxidase (SE, EC catalyzes the epoxidation of squalene to form oxidosqualene and is a key regulatory enzyme in triterpenoid saponin biosynthesis. In this study, a SE gene designated as GpSE1 was isolated from G. pentaphyllum leaves. The deduced protein sequence of GpSE1 contained two conserved domains involved in the catalytic function of SE...
December 2016: Plant Physiology and Biochemistry: PPB
Yantao Li, Wanjun Lin, Jiajun Huang, Ying Xie, Wenzhe Ma
Gynostemma pentaphyllum (Thunb.) Makino (GpM) (Jiaogulan) has been widely used in Chinese medicine for the treatment of several diseases, including hepatitis, diabetes and cardiovascular disease. Furthermore, GpM has recently been shown to exhibit potent anti-cancer activities. In this review, we have summarized recent research progress on the anti-cancer activities and mechanisms of action of GpM, as well as determining the material basis for the anti-cancer effects of GpM by searching the PubMed, Web of Science and China National Knowledge Infrastructure databases...
2016: Chinese Medicine
Lu Wang, Min Pang, Xiaobing Wang, Pan Wang, Yaping Xiao, Quanhong Liu
BACKGROUND: In order to further develop and utilise Gynostemma pentaphyllum (Thunb.) Makino seeds, a detailed analysis of the characteristics of G. pentaphyllum seed oil (GPSO), including its physico-chemical parameters, fatty acid composition and unsaponifiable matter constituents, has been investigated in this study. The antioxidant potential of GPSO was evaluated by radical-scavenging activity and ferric-reducing antioxidant power assay in vitro, and the antioxidant activity in vivo was examined by using an aged mice model...
August 28, 2016: Journal of the Science of Food and Agriculture
Xiaoya Shang, Yu Chao, Yuan Zhang, Chengyuan Lu, Chunlan Xu, Weining Niu
The immunomodulatory and antioxidant activities of crude polysaccharides extracted from Gynostemma pentaphyllum Makino (GPMPP) were investigated. GPMPP was composed of rhamnose, arabinose, xylose, mannose, glucose and galactose in the molar ratio of 1.39:3.76:1.00:1.64:4.98:5.88. In vivo studies showed GPMPP significantly increased the spleen and thymus indices, activated the macrophage phagocytosis and NK cells, and exhibited activity on none or Con A/LPS-stimulated splenocytes in a dose-dependent manner in C57BL/6 mice...
August 19, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hari Jang, Jin Woo Lee, Chul Lee, Qinghao Jin, Myung Koo Lee, Chong Kil Lee, Mi Kyeong Lee, Bang Yeon Hwang
The phytochemical investigation of the aerial parts of Gynostemma pentaphyllum led to the isolation of a new flavonol glycoside, gynopentaphylloside (1), along with seven known compounds (2-8). The structure of the new compound was determined on the basis of 1D, 2D NMR and HRESIMS data as well as acid hydrolysis. The antioxidant activity of the isolates was evaluated by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay.
September 2016: Archives of Pharmacal Research
Chenghui Liao, Kai Zheng, Yan Li, Hong Xu, Qiangrong Kang, Long Fan, Xiaopeng Hu, Zhe Jin, Yong Zeng, Xiaoli Kong, Jian Zhang, Xuli Wu, Haiqiang Wu, Lizhong Liu, Xiaohua Xiao, Yifei Wang, Zhendan He
Esophageal cancer is one of the leading cause of cancer mortality in the world. Due to the increased drug and radiation tolerance, it is urgent to develop novel anticancer agent that triggers nonapoptotic cell death to compensate for apoptosis resistance. In this study, we show that treatment with gypenoside L (Gyp-L), a saponin isolated from Gynostemma pentaphyllum, induced nonapoptotic, lysosome-associated cell death in human esophageal cancer cells. Gyp-L-induced cell death was associated with lysosomal swelling and autophagic flux inhibition...
July 26, 2016: Oncotarget
Haijie Yu, Haishan Zhang, Weihua Zhao, Liang Guo, Xueyuan Li, Yang Li, Xingang Zhang, Yingxian Sun
BACKGROUND/AIMS: Ischemia-reperfusion (I/R) injury is believed to be the major cause for detriments in coronary heart diseases, but few effective therapies for prevention or treatment of I/R injury are available. Gypenoside (GP) is the predominant effective component of Gynostemma pentaphyllum and possesses capacities against inflammation and oxidation. In the present study, the role of GP in ameliorating myocardial I/R injury was investigated. METHODS: effect GP on the cardiac structure of I/R injured rats was assessed by H&E and TTC staining...
2016: Cellular Physiology and Biochemistry
Haijie Yu, Liye Shi, Guoxian Qi, Shijie Zhao, Yuan Gao, Yuzhe Li
Gypenoside (GP) is the major effective component of Gynostemma pentaphyllum and has been shown to encompass a variety of pharmacological activities. In this study, we investigated whether GP is able to protect cardiomyocytes against injury myocardial ischemia-reperfusion (I/R) injury by using in vitro oxygen-glucose deprivation-reoxygenation (OGD/R) H9c2 cell model and in vivo myocardial I/R rat model. We found that GP pre-treatment alleviated the impairments on the cardiac structure and function in I/R injured rats...
2016: Frontiers in Pharmacology
Hui Cai, Qianlei Liang, Guanqun Ge
Reducing β amyloid- (Aβ-) induced microglial activation is believed to be effective in treating Alzheimer's disease (AD). Microglia can be activated into classic activated state (M1 state) or alternative activated state (M2 state), and the former is harmful; in contrast, the latter is beneficial. Gypenoside (GP) is the major bioactive constituent of Gynostemma pentaphyllum, a traditional Chinese herb medicine. In this study, we hypothesized that GP attenuates Aβ-induced microglial activation by ameliorating microglial M1/M2 states, and the process may be mediated by suppressor of cell signaling protein 1 (SOCS1)...
2016: Neural Plasticity
Lei Chen, Manreetpal S Brar, Frederick C C Leung, W L Wendy Hsiao
Saponins derived from medicinal plants have raised considerable interest for their preventive roles in various diseases. Here, we investigated the impacts of triterpenoid saponins isolated from Gynostemma pentaphyllum (GpS) on gut microbiome, mucosal environment, and the preventive effect on tumor growth. Six-week old ApcMin/+ mice and their wild-type littermates were fed either with vehicle or GpS daily for the duration of 8 weeks. The fecal microbiome was analyzed by enterobacterial repetitive intergenic consensus (ERIC)-PCR and 16S rRNA gene pyrosequencing...
May 24, 2016: Oncotarget
Qifa Ye, Y I Zhu, Shaojun Ye, Hong Liu, Xingguo She, Ying Niu, Yingzi Ming
Gynostemma pentaphyllum is a traditional Chinese medicine reported to possess a wide range of health benefits. As the major component of G. pentaphyllum, gypenoside (GP) displays various anti-inflammatory and anti-oxidant properties. However, it is unclear whether GP can protect against ischemia/reperfusion (I/R)-induced renal injury, and the underlying molecular mechanisms associated with this process remain unknown. In the present study, a renal I/R injury model in C57BL/6 mice was established. It was observed that, following I/R, serum concentrations of creatinine (Cr) and blood urea nitrogen (BUN) were significantly increased (P<0...
April 2016: Experimental and Therapeutic Medicine
Sumontha Nookabkaew, Nuchanart Rangkadilok, Norratouch Prachoom, Jutamaad Satayavivad
Thailand is predominantly an agriculture-based country. Organic farming is enlisted as an important national agenda to promote food safety and international export. The present study aimed to determine the concentrations of trace elements in commercial organic fertilizers (fermented and nonfermented) composed of pig and cattle manures available in Thailand. Pig and cattle manures as well as animal feeds were also collected from either animal farms or markets. The results were compared to the literature data from other countries...
April 27, 2016: Journal of Agricultural and Food Chemistry
Jia-Dong Chang, Nitin Mantri, Bin Sun, Li Jiang, Ping Chen, Bo Jiang, Zhengdong Jiang, Jialei Zhang, Jiahao Shen, Hongfei Lu, Zongsuo Liang
Recently, an important topic of research has been how climate change is seriously threatening the sustainability of agricultural production. However, there is surprisingly little experimental data regarding how elevated temperature and CO2 will affect the growth of medicinal plants and production of bioactive compounds. Here, we comprehensively analyzed the effects of elevated CO2 and temperature on the photosynthetic process, biomass, total sugars, antioxidant compounds, antioxidant capacity, and bioactive compounds of Gynostemma pentaphyllum...
June 1, 2016: Journal of Plant Physiology
Yantao Li, Jiajun Huang, Wanjun Lin, Zhongwen Yuan, Senling Feng, Ying Xie, Wenzhe Ma
Gynostemma pentaphyllum (Thunb.) Makino (GpM) has been widely used in traditional Chinese medicine (TCM) for the treatment of various diseases including cancer. Most previous studies have focused primarily on polar fractions of GpM for anticancer activities. In this study, a nonpolar fraction EA1.3A from GpM showed potent growth inhibitory activities against four cancer cell lines with IC50 ranging from 31.62 μg/mL to 38.02 μg/mL. Furthermore, EA1.3A also inhibited the growth of breast cancer cell MDA-MB-453 time-dependently, as well as its colony formation ability...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
William Chi-Shing Tai, Wing-Yan Wong, Magnolia Muk-Lan Lee, Brandon Dow Chan, Cheng Lu, Wen-Luan Wendy Hsiao
Gynostemma pentaphyllum saponins (GpS) have been shown to have anti-cancer activity. However, the underlying mechanisms remain unclear. In this study, we used the Apc(Min) (/+) colorectal cancer (CRC) mouse model to investigate the anti-cancer effect of GpS and we demonstrated that GpS treatment could significantly reduce the number and size of intestinal polyps in Apc(Min) (/+) mice. In order to identify the potential targets and mechanisms involved, a comparative proteomics analysis was performed and 40 differentially expressed proteins after GpS treatment were identified...
May 2016: Proteomics
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