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Red blood cell exchange

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https://www.readbyqxmd.com/read/29273735/the-opposing-forces-of-shear-flow-and-sphingosine-1-phosphate-control-marginal-zone-b-cell-shuttling
#1
Kerry Tedford, Michael Steiner, Stanislav Koshutin, Karin Richter, Laura Tech, Yannik Eggers, Inga Jansing, Kerstin Schilling, Anja Erika Hauser, Mark Korthals, Klaus-Dieter Fischer
Splenic marginal zone B cells (MZB) shuttle between the blood-filled marginal zone for antigen collection and the follicle for antigen delivery. However, it is unclear how MZBs migrate directionally from the marginal zone to the follicle. Here, we show that murine MZBs migrate up shear flow via the LFA-1 (αLβ2) integrin ligand ICAM-1, but adhere or migrate down the flow via the VLA-4 integrin (α4β1) ligand VCAM-1. MZBs lacking Arhgef6 (Pak-interacting exchange factor (αPIX)) or functional LFA-1 are impaired in shuttling due to mislocalization toward the VCAM-1-rich red pulp...
December 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/29250791/successful-management-of-severe-red-blood-cell-alloimmunization-in-pregnancy-with-a-combination-of-therapeutic-plasma-exchange-intravenous-immune-globulin-and-intrauterine-transfusion
#2
Laura C Nwogu, Kenneth J Moise, Kimberly L Klein, Hlaing Tint, Brian Castillo, Yu Bai
BACKGROUND: Antibodies to Rhesus and Kell antigens have been associated with severe hemolytic disease of the fetus and newborn (HDFN) necessitating intrauterine transfusion (IUT) of red blood cells (RBCs). We report a case series of five women with severe HDFN secondary to maternal RBC alloimmunization who were successfully managed with therapeutic plasma exchange (TPE), intravenous immune globulin (IVIG), and IUT. STUDY DESIGN AND METHODS: This is a retrospective case series of five women with severe HDFN who underwent a total of three TPE procedures during Weeks 10 to 13 of pregnancy, followed by weekly IVIG infusions...
December 17, 2017: Transfusion
https://www.readbyqxmd.com/read/29244812/optimal-occlusion-uniformly-partitions-red-blood-cells-fluxes-within-a-microvascular-network
#3
Shyr-Shea Chang, Shenyinying Tu, Kyung In Baek, Andrew Pietersen, Yu-Hsiu Liu, Van M Savage, Sheng-Ping L Hwang, Tzung K Hsiai, Marcus Roper
In animals, gas exchange between blood and tissues occurs in narrow vessels, whose diameter is comparable to that of a red blood cell. Red blood cells must deform to squeeze through these narrow vessels, transiently blocking or occluding the vessels they pass through. Although the dynamics of vessel occlusion have been studied extensively, it remains an open question why microvessels need to be so narrow. We study occlusive dynamics within a model microvascular network: the embryonic zebrafish trunk. We show that pressure feedbacks created when red blood cells enter the finest vessels of the trunk act together to uniformly partition red blood cells through the microvasculature...
December 15, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/29238999/physiological-gas-exchange-mapping-of-hyperpolarized-129-xe-using-spiral-ideal-and-moxe-in-a-model-of-regional-radiation-induced-lung-injury
#4
Brandon Zanette, Elaine Stirrat, Salomeh Jelveh, Andrew Hope, Giles Santyr
PURPOSE: To map physiological gas exchange parameters using dissolved hyperpolarized (HP) 129 Xe in a rat model of regional radiation-induced lung injury (RILI) with spiral-IDEAL and the model of xenon exchange (MOXE). Results are compared to quantitative histology of pulmonary tissue and red blood cell (RBC) distribution. METHODS: Two cohorts (n=6 each) of age-matched rats were used. One was irradiated in the right-medial lung, producing regional injury. Gas exchange was mapped four weeks post-irradiation by imaging dissolved-phase HP 129 Xe using spiral-IDEAL at five gas exchange timepoints using a clinical 1...
December 14, 2017: Medical Physics
https://www.readbyqxmd.com/read/29218398/enhanced-hemoglobin-oxygen-unloading-in-migratory-salmonids
#5
Jacelyn J Shu, Till S Harter, Phillip R Morrison, Colin J Brauner
Recent findings indicate that some teleost fishes may be able to greatly enhance hemoglobin-oxygen (Hb-O2) unloading at the tissues under conditions that result in catecholamine release. The putative mechanism relies on the high pH sensitivity of teleost hemoglobin (Hb), intracellular red blood cell (RBC) pH regulation via β-adrenergic Na+/H+ exchanger (β-NHE) activity, and plasma-accessible carbonic anhydrase at the tissues that short-circuits RBC pH regulation. Previous studies have shown that in rainbow trout, this system may double Hb-O2 unloading to red muscle compared to a situation without short-circuiting...
December 7, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/29196100/mathematical-calculation-of-lifespan-of-transfused-rbcs-in-sickle-cell-disease-patients
#6
Jaehyup Kim, Amena Usmani, Nicole De Simone, Ravi Sarode
BACKGROUND: Transfusion of donor red blood cells (RBCs) remains an important part of management of sickle cell disease (SCD). However, the survival characteristics of transfused donor RBCs in SCD patients have not been well studied. We sought to calculate survival kinetics of transfused RBCs in SCD patients since it is unclear whether transfused RBCs get destroyed at faster rate as innocent bystander or persist longer due to decreased destruction capacity such as functional splenectomy...
November 24, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29193101/case-series-supporting-heme-detoxification-via-therapeutic-plasma-exchange-in-acute-multiorgan-failure-syndrome-resistant-to-red-blood-cell-exchange-in-sickle-cell-disease
#7
James E Louie, Caitlin J Anderson, Katayoun Fayaz M Fomani, Alonye Henry, Trevor Killeen, Narla Mohandas, Karina Yazdanbakhsh, John D Belcher, Gregory M Vercellotti, Patricia A Shi
BACKGROUND: Depletion of haptoglobin (Hp) and hemopexin (Hx) with increase in free hemoglobin and heme are important etiologies of vaso-occlusive complications in sickle cell disease (SCD). This study is the first to show an association between clinical improvement in SCD and repletion of Hp and Hx by therapeutic plasma exchange (TPE) using plasma replacement. STUDY DESIGN AND METHODS: Thirteen fresh-frozen plasma (FFP) units derived from consecutive whole blood donations were thawed at 37°C after 10 months of storage; Hp and Hx concentrations immediately postthaw and after 5 days of refrigerated storage were analyzed by enzyme-linked immunosorbent assay (ELISA)...
November 27, 2017: Transfusion
https://www.readbyqxmd.com/read/29139162/epidemiology-of-therapeutic-apheresis-with-a-multidisciplinary-approach-at-a-high-volume-pediatric-center
#8
Rachel M Sirignano, Matthew L Paden, Ross Fasano, Erin K Meyer
INTRODUCTION: Therapeutic apheresis (TA) is used inconsistently in pediatric populations. We seek to define our multidisciplinary institutional practice. METHODS: We conducted a retrospective chart review of patients receiving TA from January 1, 2012 through October 31, 2015. Data collected included demographics, American Society of Apheresis (ASFA) indication, complications, and mortality. RESULTS: Over 46 months, 1198 TA procedures were conducted on 289 patients ranging in age from 5 months to 21 years with weights ranging from 4...
November 15, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29130518/automated-red-blood-cell-exchange-in-preparation-for-filgrastim-mobilization-of-autologous-peripheral-blood-hematopoietic-progenitor-cells-in-a-patient-with-sickle-cell-anemia
#9
Yong Zhao, Jeffrey A Bailey, Jeanne Linden, Patricia St Pierre, Jan Cerny, Michelle Vauthrin, Mindy Greene, Robert Weinstein
Increasing survival of patients with sickle cell anemia (SCA) well into adulthood results in a rising likelihood of developing hematological malignancy. High-dose chemotherapy with autologous hematopoietic progenitor cell (HPC) rescue is standard of care for several hematological malignancies, but the risk of severe or life-threatening vaso-occlusive phenomena during filgrastim mobilization of HPC for collection poses a potential barrier to this approach. We report the use of automated red cell exchange in preparation for filgrastim mobilization in a patient with homozygous SCA...
November 11, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29111410/the-z-spectrum-from-human-blood-at-7t
#10
Simon M Shah, Olivier E Mougin, Andrew J Carradus, Nicolas Geades, Richard Dury, William Morley, Penny A Gowland
Chemical Exchange Saturation Transfer (CEST) has been used to assess healthy and pathological tissue in both animals and humans. However, the CEST signal from blood has not been fully assessed. This paper presents the CEST and nuclear Overhauser enhancement (NOE) signals detected in human blood measured via z-spectrum analysis. We assessed the effects of blood oxygenation levels, haematocrit, cell structure and pH upon the z-spectrum in ex vivo human blood for different saturation powers at 7T. The data were analysed using Lorentzian difference (LD) model fitting and AREX (to compensate for changes in T1), which have been successfully used to study CEST effects in vivo...
October 27, 2017: NeuroImage
https://www.readbyqxmd.com/read/29070138/-identification-of-a-rare-ael05-b101-subtype-and-selection-of-blood-transfusion-strategy
#11
Qiang Feng, Jian-Yu Xiao, Min-Hui Wu, Yue-Xiang Zhang, Yong Xia, Xin Mu, Qing Chen
OBJECTIVE: To explore the mechanism of ABO discrepancy in a patient by ABO genotyping and the reasonable blood transfusion strategy. METHODS: Routine serological test was carried out to analyze ABO blood group. The presence of the blood group determinants on the red blood cells were determined by adsorption-elution test. Exons 1-7 and adjacent introns of the ABO gene were amplified by PCR and sequenced. RESULTS: The patient showed ABO forward and reverse typing discrepancy...
October 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29066970/hupresin-retains-binding-capacity-for-butyrylcholinesterase-and-acetylcholinesterase-after-sanitation-with-sodium-hydroxide
#12
Seda Onder, Emilie David, Ozden Tacal, Lawrence M Schopfer, Oksana Lockridge
Hupresin is a new affinity resin that binds butyrylcholinesterase (BChE) in human plasma and acetylcholinesterase (AChE) solubilized from red blood cells (RBC). Hupresin is available from the CHEMFORASE company. BChE in human plasma binds to Hupresin and is released with 0.1 M trimethylammonium bromide (TMA) with full activity and 10-15% purity. BChE immunopurified from plasma by binding to immobilized monoclonal beads has fewer contaminating proteins than the one-step Hupresin-purified BChE. However, when affinity chromatography on Hupresin follows ion exchange chromatography at pH 4...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28990205/developing-a-protocol-for-normothermic-ex-situ-limb-perfusion
#13
Eliana F R Duraes, Maria Madajka, Russell Frautschi, Basem Soliman, Cagri Cakmakoglu, Addison Barnett, Kashyap Tadisina, Qiang Liu, Patrick Grady, Cristiano Quintini, Toshihiro Okamoto, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
BACKGROUND: Ischemia time represents a significant limitation for successful extremity transplantation because of the rapid deterioration of ischemic muscle. Normothermic ex-situ preservation is an emergent method to prolong the organ viability following procurement, by replicating the physiologic conditions. The aim of this study was to develop an ex-situ normothermic limb perfusion system to preserve the viability and function of porcine limbs for 12 hours following procurement. METHODS: A total of 18 swine limbs were perfused...
October 9, 2017: Microsurgery
https://www.readbyqxmd.com/read/28981948/-identification-of-a-bw14-subtype-and-exploration-for-its-molecular-basis
#14
Qing Chen, Ping Li, Jianyu Xiao, Le Lu, Yan Ye, Shuya Wang, Chengyin Huang, Yunlong Zhuang
OBJECTIVE: To identify a rare subtype of the ABO blood group system and explore its molecular basis. METHODS: Based on a standard serological assay, ABO subtype and haplotype were analyzed through PCR amplification of the 7 exons and adjacent introns of the ABO gene and TA clone sequencing. RESULTS: Forward typing showed a B type, while reverse typing demonstrated an extremely weak anti-B on routine gel analysis, which indicated a forward and reverse typing discrepancy...
October 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28972944/delayed-development-of-hemolytic-anemia-with-fragmented-red-blood-cells-and-cardiac-and-renal-impairments-after-high-dose-chemotherapy-and-autologous-hematopoietic-stem-cell-transplantation-for-malignant-lymphoma
#15
Futoshi Iioka, Yusuke Toda, Yuya Nagai, Takashi Akasaka, Daiki Shimomura, Katsuyo Tsuda, Fumihiko Nakamura, Hitoshi Ohno
Among 42 consecutive patients with malignant lymphoma who underwent high-dose chemotherapy (HDC) followed by autologous hematopoietic stem cell transplantation (AHSCT), 5 developed hemolytic anemia with fragmented red blood cells (HA-FrRBCs) on days 87-125 (median 107) of AHSCT. Nadir Hb levels ranged between 5.0 and 6.4 g/dL with 2.2-5.6% FrRBCs. All patients developed grade ≥3 hypoxia and heart failure, and 4 developed grade ≥3 hypertension. The ejection fraction of the left ventricle assessed by echocardiography was significantly reduced in 3 patients...
October 4, 2017: Acta Haematologica
https://www.readbyqxmd.com/read/28969140/therapeutic-plasma-exchange-an-emerging-treatment-modality-in-patients-with-neurologic-and-non-neurologic-diseases
#16
Shreedevi S Bobati, Karkal Ravishankar Naik
INTRODUCTION: Therapeutic Plasma Exchange (TPE) is a procedure in which the patient's blood is passed through an apheresis machine, where the filtered plasma is removed and discarded with reinfusion of red blood cells along with replacement fluid such as plasma or albumin in to the patient. We present our experience with TPE in treatment of various neurologic and non-neurologic diseases. AIM: To evaluate TPE as primary therapy or as a first-line adjunct to other initial therapies as mentioned by American Society for Apheresis (ASFA)...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28961212/a-comprehensive-review-of-our-current-understanding-of-red-blood-cell-rbc-glycoproteins
#17
REVIEW
Takahiko Aoki
Human red blood cells (RBC), which are the cells most commonly used in the study of biological membranes, have some glycoproteins in their cell membrane. These membrane proteins are band 3 and glycophorins A-D, and some substoichiometric glycoproteins (e.g., CD44, CD47, Lu, Kell, Duffy). The oligosaccharide that band 3 contains has one N-linked oligosaccharide, and glycophorins possess mostly O-linked oligosaccharides. The end of the O-linked oligosaccharide is linked to sialic acid. In humans, this sialic acid is N-acetylneuraminic acid (NeuAc)...
September 29, 2017: Membranes
https://www.readbyqxmd.com/read/28959352/production-of-erythropoietin-specific-polyclonal-antibodies
#18
Kourosh Maboudi, Seyedeh Marzieh Hosseini, Mina Sepahi, Hashem Yaghoubi, Shahin Hadadian
BACKGROUND: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests...
March 2017: Iranian Journal of Biotechnology
https://www.readbyqxmd.com/read/28955123/evaluation-of-human-nonmercaptalbumin-as-a-marker-for-oxidative-stress-and-its-association-with-various-parameters-in-blood
#19
Rie Masudo, Keiko Yasukawa, Takahiro Nojiri, Naoyuki Yoshikawa, Hironori Shimosaka, Shinji Sone, Yumiko Oike, Akemi Ugawa, Tsutomu Yamazaki, Kentaro Shimokado, Yutaka Yatomi, Hitoshi Ikeda
Oxidative status of albumin was not a useful biomarker for oxidative stress in practical use due to time-consuming measuring method. We evaluated oxidized, human nonmercaptalbumin measured more quickly than ever by a novel method using anion-exchange HPLC. In 60 subjects taking a general health examination, mean serum human nonmercaptalbumin level was 25.1 ± 3.0% with no gender difference but positive correlation with age. There were no links between human nonmercaptalbumin and C-reactive protein, γ-glutamyltransferase or iron, reportedly associated with oxidative stress...
September 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/28942500/evaluation-of-immunostimulatory-and-immunotherapeutic-effects-of-tropical-mushroom-lentinus-edodes-against-eimeriasis-in-chicken
#20
Muhammad Irfan Ullah, Masood Akhtar, Mian Muhammad Awais, Muhammad Irfan Anwar, Kashfa Khaliq
Mushrooms (Lentinus edodes) were processed for hot water (HWE), methanolic (ME), and polysaccharide (PSE) extracts. Polysaccharides were isolated through ion exchange (DEAE cellulose) and size exclusion (Sephadex G-100) chromatography. Monosaccharides including maltose (0.282%), glucose (0.113%), and mannose (0.451%) were identified, qualitatively and quantitatively, from the isolated polysaccharides through high-performance liquid chromatography. The whole study was divided into two experiments. Experiment 1 was meant for the evaluation of HWE and ME; whereas, experiment 2 was meant for the evaluation of PSE for immunostimulatory and immunotherapeutic activities...
September 24, 2017: Tropical Animal Health and Production
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