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https://www.readbyqxmd.com/read/28651008/production-and-purification-of-human-hsp90%C3%AE-in-escherichia-coli
#1
Martina Radli, Dmitry B Veprintsev, Stefan G D Rüdiger
The molecular chaperone Hsp90 is an essential member of the cellular proteostasis system. It plays an important role in the stabilisation and activation of a large number of client proteins and is involved in fatal disease processes, e.g. Alzheimer disease, cancer and cystic fibrosis. This makes Hsp90 a crucial protein to study. Mechanistic studies require large amounts of protein but the production and purification of recombinant human Hsp90 in Escherichia coli is challenging and laborious. Here we identified conditions that influence Hsp90 production, and optimised a fast and efficient purification protocol...
2017: PloS One
https://www.readbyqxmd.com/read/28650492/exploiting-the-biosynthetic-machinery-of-streptomyces-pilosus-to-engineer-a-water-soluble-zirconium-iv-chelator
#2
Tomas Richardson-Sanchez, William Tieu, Michael P Gotsbacher, Thomas J Telfer, Rachel Codd
The water solubility of a natural product-inspired octadentate hydroxamic acid chelator designed to coordinate Zr(iv)-89 has been improved by using a combined microbiological-chemical approach to engineer four ether oxygen atoms into the main-chain region of a methylene-containing analogue. First, an analogue of the trimeric hydroxamic acid desferrioxamine B (DFOB) that contained three main-chain ether oxygen atoms (DFOB-O3) was generated from cultures of the native DFOB-producer Streptomyces pilosus supplemented with oxybis(ethanamine) (OBEA), which competed against the native 1,5-diaminopentane (DP) substrate during DFOB assembly...
June 26, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28648957/teaching-psychology-to-nursing-students-a-discussion-of-the-potential-contribution-of-psychology-towards-building-resilience-to-lapses-in-compassionate-caring
#3
Jan M A de Vries, Fiona Timmins
Psychology is a required element in nursing education in many countries. It is particularly aimed at teaching nursing students to get a better understanding of patients, colleagues, health care organizations and themselves, and moreover to apply what they learn about psychology to optimise their care. A meaningful integration of psychology within nursing education requires an emphasis on its application in understanding aspects of care and skills development. However, its ultimate value is demonstrated when addressing problem areas in nursing and health care...
June 17, 2017: Nurse Education in Practice
https://www.readbyqxmd.com/read/28648701/universal-genomic-testing-the-next-step-in-oncological-decision-making-or-a-dead-end-street
#4
Julian Walter Holch, Klaus Hans Metzeler, Andreas Jung, Kristina Riedmann, Philipp Jakob Jost, Wilko Weichert, Thomas Kirchner, Volker Heinemann, Christoph Benedikt Westphalen
The concept of 'personalised medicine' aims at allocating patients to different treatment options based on individual characteristics to optimise treatment benefit and side effects. In oncology, personalised treatments coupled to biomarkers have led to the approval of targeted agents with high anti-tumour activity. However, these therapies are often limited to narrow, molecularly defined subsets of patients with a specific morphomolecular tumour profile. Recently, it became obvious that the same molecular alteration might drive oncogenesis in many different tumours, and it might be beneficial to target the alteration in a histology informed but entity-overarching way...
June 22, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28648698/three-year-pooled-analysis-of-factors-associated-with-clinical-outcomes-across-dabrafenib-and-trametinib-combination-therapy-phase-3-randomised-trials
#5
Dirk Schadendorf, Georgina V Long, Daniil Stroiakovski, Boguslawa Karaszewska, Axel Hauschild, Evgeny Levchenko, Vanna Chiarion-Sileni, Jacob Schachter, Claus Garbe, Caroline Dutriaux, Helen Gogas, Mario Mandalà, John B A G Haanen, Céleste Lebbé, Andrzej Mackiewicz, Piotr Rutkowski, Jean-Jacques Grob, Paul Nathan, Antoni Ribas, Michael A Davies, Ying Zhang, Mathilde Kaper, Bijoyesh Mookerjee, Jeffrey J Legos, Keith T Flaherty, Caroline Robert
AIM: Understanding predictors of long-term benefit with currently available melanoma therapies is the key for optimising individualised treatments. A prior pooled analysis of dabrafenib plus trametinib (D + T)-randomised trials (median follow-up, 20.0 months) identified baseline lactate dehydrogenase (LDH) and number of organ sites with metastasis as predictive factors for progression-free (PFS) and overall (OS) survival. However, longer-term follow-up analyses are needed to confirm which patients treated with D + T can achieve maximum benefit...
June 21, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28648670/the-application-of-atherogenic-dyslipidaemia-consensus-recommendations-in-the-primary-care-setting-dat-ap-study
#6
Angel Díaz Rodríguez, Adalberto Serrano Cumplido, Jesús Millán Nuñez-Cortés, Marta Rodríguez de Miguel, Mª Luisa Orera-Peña
BACKGROUND AND AIMS: The "DAT-AP" (from the Spanish, "Dislipemia ATerogénica en Atención Primaria", for Atherogenic Dyslipidaemia in Primary Care) study objective is to determine to what extent published consensus guidelines for the diagnostic and therapeutic management of AD are used in the primary care setting, and to evaluate the approach of the participating physicians towards the detection, diagnosis, and treatment of AD. METHODS: This is descriptive, cross-sectional, multicentre study performed between January and May 2015 in primary care centres throughout Spain...
June 22, 2017: Clínica e Investigación en Arteriosclerosis
https://www.readbyqxmd.com/read/28648615/structural-biology-and-the-design-of-new-therapeutics-from-hiv-and-cancer-to-mycobacterial-infections
#7
REVIEW
Sherine E Thomas, Vitor Mendes, So Yeon Kim, Sony Malhotra, Bernardo Ochoa-Montaño, Michal Blaszczyk, Tom L Blundell
Interest in applications of protein crystallography to medicine was evident as the first high-resolution structures emerged in the 50s and 60s. In Cambridge Max Perutz and John Kendrew sought to understand mutations in sickle cell and other genetic diseases related to haemoglobin, while in Oxford the group of Dorothy Hodgkin became interested in long-lasting zinc-insulin crystals for treatment of diabetes and later considered insulin redesign as synthetic insulins became possible. The use of protein crystallography in structure-guided drug discovery emerged as enzyme structures allowed the identification of potential inhibitor-binding sites and optimisation of interactions of hits using the structure of the target protein...
June 22, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28648471/optimisation-of-continuous-gas-fermentation-by-immobilisation-of-acetate-producing-acetobacterium-woodii
#8
Franziska Steger, Lydia Rachbauer, Matthias Windhagauer, Lucy F R Montgomery, Günther Bochmann
Hydrogen from water electrolysis is often suggested as a way of storing the excess energy from wind and solar power plants. However, unlike natural gas, hydrogen is difficult to store and distribute. One solution is to convert the hydrogen into other fuels or bulk chemicals. In this study we investigated fermentation in which homoacetogenic clostridia apply the Wood-Ljungdahl pathway to generate acetate from H2 and CO2. Acetate can be used as a bulk chemical or further transformed into biofuels. Autotrophic growth with CO2 as the sole carbon source is slow compared to heterotrophic growth, so the aim of this work was to improve continuous gas fermentation by immobilising the acetate-producing clostridia, thus preventing their wash out from the bioreactor...
June 22, 2017: Anaerobe
https://www.readbyqxmd.com/read/28648187/-role-of-the-nurse-in-the-case-of-complex-hand-wounds
#9
REVIEW
Camille Choufani, Laurent Mathieu, Naklan Ouattara, Hugues Lefort
The initial assessment and care determine the quality of the secondary surgical treatment of complex hand wounds. The caregiver providing this initial care plays a critical role. The analysis of the management of these traumas in exceptional situations enables the key stages of the initial treatment to be identified: bleeding control, infection prevention and an accurate assessment of the injury. These measures thereby help to optimise the patient's transfer towards a specialised unit.
June 2017: Soins; la Revue de Référence Infirmière
https://www.readbyqxmd.com/read/28648127/specialist-palliative-care-nurses-management-of-the-needs-of-patients-with-depression
#10
Jessica Elizabeth Hayes, Bethne Hart, Jane Phillips
BACKGROUND: Depression is an important condition to consider if we are to optimise the care outcomes for patients with palliative care needs. Depression has a high incidence in palliative patients, with up to 15% diagnosed with major depression and 37% expressing some form of depressive symptoms ( O'Connor et al, 2010 ). The challenge is to ensure that palliative care patients with depression are identified in a timely manner and that their depression is effectively managed. AIM: To examine how Australian specialist inpatient palliative care nurses perceive, assess and respond to depression in a patient case study...
June 2, 2017: International Journal of Palliative Nursing
https://www.readbyqxmd.com/read/28647551/factors-predicting-falls-and-mobility-outcomes-in-patients-with-stroke-returning-home-after-rehabilitation-who-are-at-risk-of-falling
#11
Mandy Man-Di Ng, Keith D Hill, Frances Batchelor, Elissa Burton
OBJECTIVE: To identify factors predicting falls and limited mobility in people with stroke at 12 months after returning home from rehabilitation. DESIGN: Observational cohort study with 12 month follow-up. SETTING: Community. PARTICIPANTS: People with stroke (n=144) and increased falls risk discharged home from rehabilitation. INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURES: Falls were measured using monthly calendars completed by participants, and mobility was assessed using gait speed over five metres (high mobility (>0...
June 21, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28647146/analysis-of-oxidised-heavy-paraffininc-products-by-high-temperature-comprehensive-two-dimensional-gas-chromatography
#12
H Potgieter, R Bekker, J Beigley, E Rohwer
Heavy petroleum fractions are produced during crude and synthetic crude oil refining processes and they need to be upgraded to useable products to increase their market value. Usually these fractions are upgraded to fuel products by hydrocracking, hydroisomerization and hydrogenation processes. These fractions are also upgraded to other high value commercial products like lubricant oils and waxes by distillation, hydrogenation, and oxidation and/or blending. Oxidation of hydrogenated heavy paraffinic fractions produces high value products that contain a variety of oxygenates and the characterization of these heavy oxygenates is very important for the control of oxidation processes...
June 16, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28646107/become-one-with-the-force-optimising-mechanotherapy-through-an-understanding-of-mechanobiology
#13
EDITORIAL
Stuart J Warden, William R Thompson
No abstract text is available yet for this article.
July 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28646106/optimising-load-to-optimise-outcomes
#14
EDITORIAL
Philip Glasgow
No abstract text is available yet for this article.
July 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28645327/impact-of-growth-matrix-on-pharmacodynamics-of-antimicrobial-drugs-for-pig-pneumonia-pathogens
#15
Lucy Dorey, Peter Lees
BACKGROUND: The most widely used measure of potency of antimicrobial drugs is Minimum Inhibitory Concentration (MIC). MIC is usually determined under standardised conditions in broths formulated to optimise bacterial growth on a species-by-species basis. This ensures comparability of data between laboratories. However, differences in values of MIC may arise between broths of differing chemical composition and for some drug classes major differences occur between broths and biological fluids such as serum and inflammatory exudate...
June 23, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28644896/how-fast-is-a-collective-bacterial-state-established
#16
Mikkel Lindstrøm Sørensen, Peter Dahl, Thomas Sams
Bacteria in a biofilm colony have the capacity to monitor the size and growth conditions for the colony and modify their phenotypical behaviour to optimise attacks, defence, migration, etc. The quorum sensing systems controlling this involve production and sensing of diffusive signal molecules. Frequently, quorum sensing systems carry a positive feedback loop which produces a switch at a threshold size of the colony. This all-or-none switch can be beneficial to create a sudden attack, leaving a host little time to establish a defence...
2017: PloS One
https://www.readbyqxmd.com/read/28642125/safety-and-efficacy-of-dual-lead-thalamic-deep-brain-stimulation-for-patients-with-treatment-refractory-multiple-sclerosis-tremor-a-single-centre-randomised-single-blind-pilot-trial
#17
Seth F Oliveria, Ramon L Rodriguez, Dawn Bowers, Daniel Kantor, Justin D Hilliard, Erin H Monari, Bonnie M Scott, Michael S Okun, Kelly D Foote
BACKGROUND: Efficacy in previous studies of surgical treatments of refractory multiple sclerosis tremor using lesioning or deep brain stimulation (DBS) has been variable. The aim of this study was to investigate the safety and efficacy of dual-lead thalamic DBS (one targeting the ventralis intermedius-ventralis oralis posterior nucleus border [the VIM lead] and one targeting the ventralis oralis anterior-ventralis oralis posterior border [the VO lead]) for the treatment of multiple sclerosis tremor...
June 19, 2017: Lancet Neurology
https://www.readbyqxmd.com/read/28641903/-effectiveness-of-physical-exercise-on-fitness-in-frail-older-adults-a-systematic-review-of-randomised-trials
#18
Maria Viladrosa, Carles Casanova, Angela Claudia Ghiorghies, Pilar Jürschik
Performing exercise to maintain a good physical condition is crucial to improve and prevent frailty in older adults. The aim of this review was to assess the beneficial effects of physical exercise on fitness in frail older adults. A thorough literature search of randomised clinical trials (RCT) in the last 15 years was performed on different electronic databases. The methodological assessment of studies was obtained using the PEDro scale. Ten RCT were included, providing a final sample of 1,130 individuals...
June 19, 2017: Revista Española de Geriatría y Gerontología
https://www.readbyqxmd.com/read/28640926/clinimetric-properties-of-hip-abduction-strength-measurements-obtained-using-a-handheld-dynamometer-in-individuals-with-a-lower-extremity-amputation
#19
Ruud A Leijendekkers, Gerben van Hinte, Amy D Sman, J Bart Staal, Maria W G Nijhuis-van der Sanden, Thomas J Hoogeboom
INTRODUCTION: Suitable handheld dynamometer (HHD)-techniques to test hip abduction strength in individuals with a lower extremity amputation, irrespective of their amputation level are absent. The aim of this study was to optimise a HHD-technique and to test its reproducibility and validity. METHODS: This study involved three phases, in which two techniques were evaluated. Both HHD-techniques used a lever-arm of 22 centimetre. HHD-technique 1 used a break-technique...
2017: PloS One
https://www.readbyqxmd.com/read/28639355/how-do-the-characteristics-of-breast-cancer-diagnostic-assessment-programmes-influence-service-delivery-a-mixed-methods-study
#20
A R Gagliardi, G Honein-AbouHaidar, T Stuart-McEwan, J Smylie, A Arnaout, J Seely, F C Wright, M J Dobrow, M C Brouwers, K Bukhanov, D R McCready
Diagnostic assessment programmes (DAPs) coordinate multidisciplinary teamwork (MDT), and improve wait times and patient satisfaction. No research has established optimal DAP design. This study explored how DAP characteristics influence service delivery. A mixed methods case study of four breast cancer DAPs was conducted including qualitative interviews with health-care providers and retrospective chart review. Data were integrated using multiple approaches. Twenty-three providers were interviewed; 411 medical records were reviewed...
June 21, 2017: European Journal of Cancer Care
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