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https://www.readbyqxmd.com/read/28644763/methyltransferase-g9a-regulates-osteogenesis-via-twist-gene-repression
#1
N Higashihori, B Lehnertz, A Sampaio, T M Underhill, F Rossi, J M Richman
Here we investigate the role of epigenetic factors in controlling the timing of cranial neural crest cell differentiation. The gene coding for histone H3 lysine 9 methyltransferase G9A was conditionally deleted in neural crest cells with Wnt1-Cre. The majority of homozygous-null animals survived to birth but thereafter failed to thrive. Phenotypic analysis of postnatal animals revealed that the mutants displayed incomplete ossification and 20% shorter jaws as compared to their wild-type littermates. At E13...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28643928/calvarial-bone-development-and-suture-closure-in-dicer-deficient-mice
#2
P Atsawasuwan, M Ouibaidin, B Dalal, H Khan, A Mohammed
OBJECTIVE: To evaluate whether lack of Dicer during calvaria development would lead to dysmorphology of calvaria and suture closure in mice. MATERIALS AND METHODS: A conditional Dicer deficient under Osx promoter mouse was employed in this study. The 4- and 10-week-old conditional Dicer-deficient mice control littermates and Osx-cre transgenic mice were studied for calvarial bone morphology and suture closure. Dry skull, microcomputed tomography (μCT), histological and gene expression studies were investigated to evaluate the effect of Dicer deficiency on calvarial bone morphology and their related genes during calvaria development...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28643917/estrogen-receptor-alpha-mediates-mandibular-condylar-cartilage-growth-in-male-mice
#3
J L Robinson, V Gupta, P Soria, E Clanaman, S Gurbarg, M Xu, J Chen, S Wadhwa
OBJECTIVES: In the appendicular skeleton, estrogen via ERα signalling has been shown to mediate endochondral growth plate fusion in both males and females. However, the role of ERα in mediating growth of the mandibular condylar cartilage is unknown. Thus, this study focuses on the characterization of the mandibular condylar cartilage phenotype in young and adult male ERαKO mice. SETTING: Columbia University Medical Center. MATERIAL AND METHODS: WT and ERαKO C57BL/6 male mice were sacrificed at 49 days or 9 months for phenotypic analysis...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28643442/the-effect-of-collagen-coating-on-titanium-with-nanotopography-on-in-vitro-osteogenesis
#4
Daniel G Costa, Emanuela P Ferraz, Rodrigo P F Abuna, Paulo T de Oliveira, Marco Morra, Marcio M Beloti, Adalberto L Rosa
Several studies have shown the positive effects of Ti either with nanotopography or coated with collagen on osteoblast differentiation. Thus, we hypothesized that the association of nanotopography with collagen may increase the in vitro osteogenesis on Ti surface. Ti discs with nanotopography with or without collagen coating were characterized by scanning electron microscopy and atomic force microscopy. Rat calvaria-derived osteoblastic cells were cultured on both Ti surfaces for up to 14 days and the following parameters were evaluated: cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization, protein expression of bone sialoprotein (BSP) and osteopontin (OPN), and gene expression of collagen type 1a (Coll1a), runt related transcription factor 2 (Runx2), osterix (OSX), osteocalcin (OC), Ki67, Survivin and Bcl2 associated X protein (BAX)...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28639943/bioprintable-form-of-chitosan-hydrogel-for-bone-tissue-engineering
#5
T Tolga Demirtas, Gülseren Irmak, Menemse Gumusderelioglu
Bioprinting can be defined as the 3D patterning of living cells and other biologics by using a computer-aided layer-by-layer deposition approach. Presence of cells within the ink to usage of a "bio-ink" presents the potential to print of living tissues and organ analogs that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration. In this study, it was shown for the first time that chitosan solution and its composite with nanostructured bone-like hydroxyapatite (HA) can be mixed with cells and printed successfully...
June 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28639404/mechanoresponsiveness-of-human-adipose-stem-cells-on-nanocomposite-and-micro-hybrid-composite
#6
Fransisca A S van Esterik, Samaneh Ghazanfari, Behrouz Zandieh-Doulabi, Cornelis M Semeins, Cornelis J Kleverlaan, Jenneke Klein-Nulend
Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared to micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared to micrometer-sized filler particles...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28639378/regulation-of-the-fate-of-dental-derived-mesenchymal-stem-cells-using-engineered-alginate-gelma-hydrogels
#7
Sahar Ansari, Patricia Sarrion, Mohammad Mahdi Hasani-Sadrabdi, Tara Aghaloo, Benjamin M Wu, Alireza Moshaverinia
Mesenchymal stem cells (MSCs) derived from dental and orofacial tissues provide an alternative therapeutic option for craniofacial bone tissue regeneration. However, there is still a need to improve stem cell delivery vehicles to regulate the fate of the encapsulated MSCs for high quality tissue regeneration. Matrix elasticity plays a vital role in MSC fate determination. Here we have prepared various hydrogel formulations based on alginate and gelatin methacryloyl (GelMA) and have encapsulated gingival mesenchymal stem cells (GMSCs) and human bone marrow MSCs (hBMMSCs) within these fabricated hydrogels...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28637981/effects-of-vascular-endothelial-growth-factor-on-osteoblasts-around-dental-implants-in-vitro-and-in-vivo
#8
Mengkai Guang, Bo Huang, Yang Yao, Liang Zhang, Bo Yang, Ping Gong
Vasculogenesis is a pivotal procedure during dental implant osseointegration and bone repair process. Vascular endothelial growth factor (VEGF), regarded as one of the most important vasculogenesis factor, also plays a central role in bone repair, but its role around dental implants is still unknown. In the present study, rat primary osteoblasts seeded on titanium discs were tested using proliferation, enzyme-linked immunosorbent assay, Real-time PCR, and alkaline phosphatase (ALP) expression. Chicken embryo chorioallantoic membrane (CAM) was used to test the vasculogenesis property...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/28631378/runx2-i-is-an-early-regulator-of-epithelial-mesenchymal-cell-transition-in-the-chick-embryo
#9
Andre L P Tavares, Jessie A Brown, Emily C Ulrich, Katerina Dvorak, Raymond B Runyan
BACKGROUND: Though normally linked to bone and cartilage development, the Runt-related transcription factor, RUNX2, was reported in the mouse heart during development of the valves. We examined RUNX2 expression and function in the developing avian heart as it related to the epithelial-mesenchymal transition (EMT) in the atrioventricular canal. EMT can be separated into an activation stage involving hypertrophy and cell separation and an invasion stage where cells invade the extracellular matrix...
June 19, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28627829/mir-29a-and-messenger-rna-expression-of-bone-turnover-markers-in-canonical-wnt-pathway-in-patients-with-ankylosing-spondylitis
#10
Jinxian Huang, Guoxiang Song, Zhihua Yin, Zhongchao Fu, Zhizhong Ye
BACKGROUND: Recent studies showed that the canonical Wnt pathway and miR-29a play important roles in the pathogenesis of bone formation. We studied the levels of miR-29a and messenger RNA (mRNA) of bone turnover markers in the canonical Wnt pathway in ankylosing spondylitis (AS). METHODS: The levels of miR-29a and mRNA of bone turnover markers in canonical Wnt pathway from peripheral blood mononuclear cells were determined by real-time quantitative polymerase chain reaction in 38 patients with AS and 32 healthy controls...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28627701/mangiferin-positively-regulates-osteoblast-differentiation-and-suppresses-osteoclast-differentiation
#11
Yuusuke Sekiguchi, Hiroshi Mano, Sachie Nakatani, Jun Shimizu, Aya Kataoka, Kana Ogura, Yoshifumi Kimira, Midori Ebata, Masahiro Wada
Mangiferin is a polyphenolic compound present in Salacia reticulata. It has been reported to reduce bone destruction and inhibit osteoclastic differentiation. This study aimed to determine whether mangiferin directly affects osteoblast and osteoclast proliferation and differentiation, and gene expression in MC3T3‑E1 osteoblastic cells and osteoclast‑like cells derived from primary mouse bone marrow macrophage cells. Mangiferin induced significantly greater WST‑1 activity, indicating increased cell proliferation...
June 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28624869/incorporation-of-cerium-oxide-into-hydroxyapatite-coating-protects-bone-marrow-stromal-cells-against-h2o2-induced-inhibition-of-osteogenic-differentiation
#12
Kai Li, Qingyi Shen, Youtao Xie, Mingyu You, Liping Huang, Xuebin Zheng
Oxidative stress exerts a key influence in osteoporosis in part by inhibiting osteogenic differentiation of bone marrow stromal cells (BMSCs). With their unique antioxidant properties and reported biocompatibility, cerium oxide (CeO2) ceramics exhibit promising potential for the treatment of osteoporosis resulting from oxidative stress. In this study, protective effects of CeO2-incorporated hydroxyapatite coatings (HA-10Ce and HA-30Ce) on the viability and osteogenic differentiation of H2O2-treated BMSCs were examined...
June 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#13
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28622088/engineering-osteoinductive-biomaterials-by-bioinspired-synthesis-of-apatite-coatings-on-collagen-hydrogels-with-varied-pore-microarchitectures
#14
Jacqueline Lorraine Harding, Matthew Osmond, Melissa Diane Krebs
Biomaterial controlled osteoinduction is influenced by the porous microenvironment and the composition of incorporated calcium orthophosphate (CaPi) polymorphs, however for the design of materials that rival the efficacies of natural grafts a systematic approach to assessing the physicochemical properties that affects cellular differentiation is needed. In this research, we introduce a bioinspired synthetic approach to the mineralization of preformed porous collagen hydrogel scaffolds with tunable apatite coatings...
June 16, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28622009/mir-139-5p-represses-bmsc-osteogenesis-via-targeting-wnt-%C3%AE-catenin-signaling-pathway
#15
Haitao Long, Buhua Sun, Liang Cheng, Shushan Zhao, Yong Zhu, Ruibo Zhao, Jianxi Zhu
Osteogenesis of mesenchymal stem cells (MSCs) has played a necessary role in the repair of bone. According to some reports, microRNAs participate in different physiological activity of the cells, including cell differentiation. This study investigated the function that miR-139-5p plays in the osteogenic differentiation of human bone marrow MSCs (hBMSCs). In addition to miR-139-5p, the effects of alkaline phosphatase (ALP), a membrane-bound metalloenzyme that is considered an early osteogenic differentiation marker, have also been investigated...
June 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28621459/microtubule-actin-crosslinking-factor-1-promotes-osteoblast-differentiation-by-promoting-%C3%AE-catenin-tcf1-runx2-signaling-axis
#16
Lifang Hu, Peihong Su, Chong Yin, Yan Zhang, Runzhi Li, Kun Yan, Zhihao Chen, Dijie Li, Ge Zhang, Liping Wang, Zhiping Miao, Airong Qian, Cory J Xian
Osteoblast differentiation is a multistep process delicately regulated by many factors, including cytoskeletal dynamics and signaling pathways. Microtubule actin crosslinking factor 1 (MACF1), a key cytoskeletal linker, has been shown to play key roles in signal transduction and in diverse cellular processes; however, its role in regulating osteoblast differentiation is still needed to be elucidated. To further uncover the functions and mechanisms of action of MACF1 in osteoblast differentiation, we examined effects of MACF1 knockdown (MACF1-KD) in MC3T3-E1 osteoblastic cells on their osteoblast differentiation and associated molecular mechanisms...
June 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28621452/osteogenic-commitment-and-differentiation-of-human-mesenchymal-stem-cells-by-low-intensity-pulsed-ultrasound-stimulation
#17
Viviana Costa, Valeria Carina, Simona Fontana, Angela De Luca, Francesca Monteleone, Stefania Pagani, Maria Sartori, Stefania Setti, Cesare Faldini, Riccardo Alessandro, Milena Fini, Gianluca Giavaresi
Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis...
June 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28615725/transcriptome-analysis-reveals-similarities-between-human-blood-cd3-cd56-bright-cells-and-mouse-cd127-innate-lymphoid-cells
#18
David S J Allan, Ana Sofia Cerdeira, Anuisa Ranjan, Christina L Kirkham, Oscar A Aguilar, Miho Tanaka, Richard W Childs, Cynthia E Dunbar, Jack L Strominger, Hernan D Kopcow, James R Carlyle
For many years, human peripheral blood natural killer (NK) cells have been divided into functionally distinct CD3(-) CD56(bright) CD16(-) and CD3(-) CD56(dim) CD16(+) subsets. Recently, several groups of innate lymphoid cells (ILC), distinct from NK cells in development and function, have been defined in mouse. A signature of genes present in mouse ILC except NK cells, defined by Immunological Genome Project studies, is significantly over-represented in human CD56(bright) cells, by gene set enrichment analysis...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28611356/the-osteogenesis-promoting-effects-of-alpha-lipoic-acid-against-glucocorticoid-induced-osteoporosis-through-the-nox4-nf-kappab-jnk-and-pi3k-akt-pathways
#19
Shi-Yu Lu, Chang-Yuan Wang, Yue Jin, Qiang Meng, Qi Liu, Zhi-Hao Liu, Ke-Xin Liu, Hui-Jun Sun, Mo-Zhen Liu
Recently, accumulating evidence has indicated that glucocorticoid-induced osteoporosis (GIOP) is closely related to oxidative stress and apoptosis. Alpha-lipoic acid (LA), a naturally endogenous anti-oxidant, possesses anti-oxidative and anti-apoptosis activities, implicating LA as a therapeutic agent for the treatment of GIOP. In this study, the osteogenesis-promoting effects of LA against GIOP were investigated and the mechanisms were further probed. Here, the results showed that LA inhibited oxidative stress, suppressed apoptosis and improved osteopenia by promoting the expression of osteogenesis markers, including ALP, COL-I, OCN, BMP-2, RUNX2 and OSX...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608802/enhanced-osteogenic-differentiation-in-zoledronate-treated-osteoporotic-patients
#20
Luca Dalle Carbonare, Monica Mottes, Giovanni Malerba, Antonio Mori, Martina Zaninotto, Mario Plebani, Alessandra Dellantonio, Maria Teresa Valenti
Bisphosphonates are well known inhibitors of osteoclast activity and thus may be employed to influence osteoblast activity. The present study was designed to evaluate the in vivo effects of zoledronic acid (ZA) on the proliferation and osteoblastic commitment of mesenchymal stem cells (MSC) in osteoporotic patients. We studied 22 postmenopausal osteoporotic patients. Densitometric, biochemical, cellular and molecular data were collected before as well as after 6 and 12 months of ZA treatment. Peripheral blood MSC-like cells were quantified by colony-forming unit fibroblastic assay; their osteogenic differentiation potential was evaluated after 3 and 7 days of induction, respectively...
June 13, 2017: International Journal of Molecular Sciences
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