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https://www.readbyqxmd.com/read/28928968/evaluation-of-the-effects-of-dimethylsulphoxide-on-morphology-cellular-viability-mrna-and-protein-expression-of-stem-cells-culture-in-growth-media
#1
Hyunjin Lee, Jun-Beom Park
The aim of this study was to evaluate the effects of differential concentration of dimethylsulphoxide (DMSO) on the morphology, cell viability, mRNA, and protein expression of stem cells obtained from the intraoral area. Stem cells derived obtained from gingiva were cultured in a growth medium in the presence of DMSO at concentrations ranging from 0.01 to 10%. The morphology and cellular viability were evaluated on days 1, 3, 5, 7 and 10. Quantitative polymerase chain reaction was used to evaluate the mRNA levels of collagen I and Runt-related transcription factor 2 (Runx2)...
October 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28927744/impact-of-resveratrol-on-bone-repair-in-rats-exposed-to-cigarette-smoke-inhalation-histomorphometric-and-bone-related-gene-expression-analysis
#2
F C Franck, B B Benatti, D C Andia, F R Cirano, R C Casarin, M G Corrêa, F V Ribeiro
This study investigated the effect of resveratrol on bone healing and its influence on the gene expression of bone-related markers in rats exposed to cigarette smoke. Two calvarial defects were created in each of 60 rats, which were assigned equally (n=20) to three groups: (1) resveratrol (10mg/kg)+smoke exposure (SMK+RESV); (2) placebo+smoke exposure (SMK+PLA); or (3) placebo+no smoke exposure (NS+PLA). Substances were administered daily for 30days following surgery. Smoke inhalation was started 7days before surgery and continued for 30days after surgery...
September 15, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28924102/microrna-483-5p-modulates-the-expression-of-cartilage-related-genes-in-human-chondrocytes-through-down-regulating-tgf-%C3%AE-1-expression
#3
Ronghua Xu, Jiayi Li, Bo Wei, Weiling Huo, Liming Wang
Transforming growth factor-β1 (TGF-β1) has been reported to improve chondrocytes phenotype and function. The expression levels of microRNA-483-5p (miR-483-5p), a potential regulator of TGF-β signaling pathway, were elevated in chondrocytes of patients with osteoarthritis. In this study, we aimed to explore the role of miR-483-5p for the expression of cartilage-related genes in chondrocytes. Human chondrocytes were harvested from femoral condyle and tibial plateau of different donors (n = 10) following amputation due to sarcomas not involving the joint space...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28921615/plasticizer-di-2-ethylhexyl-phthalate-interferes-with-osteoblastogenesis-and-adipogenesis-in-a-mouse-model
#4
Chen-Yuan Chiu, Shih-Chun Sun, Chih-Kang Chiang, Ching-Chia Wang, Ding-Cheng Chan, Huang-Jen Chen, Shing-Hwa Liu, Rong-Sen Yang
Plasticizer di(2-ethylhexyl)phthalate (DEHP) can leach from medical devices such as blood storage bags and the tubing. Recently, epidemiological studies showed that phthalate metabolites levels in the urine are associated with low bone mineral density (BMD) in older women. The detailed effect and mechanism of DEHP on osteoblastogenesis and adipogenesis and bone loss remain to be clarified. Here, we investigated the effect and mechanism of DEHP and its active metabolite mono(2 -ethylhexyl)phthalate (MEHP) on osteoblastogenesis and adipogenesis...
September 16, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28919027/macrolactin-f-inhibits-rankl-mediated-osteoclastogenesis-by-suppressing-akt-mapk-and-nfatc1-pathways-and-promotes-osteoblastogenesis-through-a-bmp-2-smad-akt-runx2-signaling-pathway
#5
Liang Li, Mahesh Sapkota, Ming Gao, Hyukjae Choi, Yunjo Soh
The balance between bone formation and bone resorption is maintained by osteoblasts and osteoclasts. In the current study, macrolactin F (MF) was investigated for novel biological activity on the receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclastogenesis in primary bone marrow-derived macrophages (BMMs). We found that RANKL-induced osteoclast formation and differentiation from BMMs was significantly inhibited by MF in a dose-dependent manner without cytotoxicity. RANKL-induced F-actin ring formation and bone resorption activity in BMMs which was attenuated by MF...
September 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28913955/controlled-jagged1-delivery-induces-human-embryonic-palate-mesenchymal-cells-to-form-osteoblasts
#6
Jean De La Croix Ndong, Stephenson Yvonne, Michael E Davis, Andrés J García, Steven Goudy
Osteoblast commitment and differentiation are controlled by multiple growth factors including members of the Notch signaling pathway. JAGGED1 is a cell surface ligand of the Notch pathway that is necessary for murine bone formation. The delivery of JAGGED1 to induce bone formation is complicated by its need to be presented in a bound form to allow for proper Notch receptor signaling. In this study we investigate whether the sustained release of JAGGED1 stimulates human mesenchymal cells to commit to osteoblast cell fate using polyethylene glycol malemeide (PEG-MAL) hydrogel delivery system...
September 15, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28903624/repair-of-calvarial-bone-defect-using-jarid1a-knockdown-bone-mesenchymal-stem-cells-in-rats
#7
Yuan Deng, Tao Guo, Jipeng Li, Li Guo, Ping Gu, Xianqun Fan
Histone methylation is regarded as an important epigenetic event during stem cell differentiation. Jumonji AT-rich interactive domain 1A (Jarid1a) is a histone demethylase that specifically catalyzes demethylation of dimethyl or trimethyl histone H3K4me3, which is normally associated with transcriptionally active genes. Runt-related transcription factor 2 (Runx2) has been identified as a key transcription factor in the early stage of osteogenesis. A better understanding of this epigenetic mechanism that governs osteogenic differentiation of bone mesenchymal stem cells (BMSCs) can provide new insights into BMSCs based bone tissue engineering...
September 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28901520/puerarin-promotes-the-viability-and-differentiation-of-mc3t3%C3%A2-e1-cells-by-mir%C3%A2-204%C3%A2-regulated-runx2-upregulation
#8
Xiu-Qin Zhan, Xiang-Wei Zeng, Ying-Ying Zhang, Qian Feng, Feng-Ming Zhao, Ze-Qun Jiang, Chao Sun
Puerarin has attracted increasing attention because of its beneficial effects on anti‑osteoporosis, but the molecular mechanisms underlying its actions on osteoblasts are not fully understood. The current study aimed to investigate the effect of puerarin on the cell viability and differentiation of mouse MC3T3‑E1 osteoblast‑like cells in vitro and its underlying mechanisms. The results indicated that 0.01, 0.1 and 1 mg/ml puerarin significantly promoted the viability of osteoblasts, enhanced alkaline phosphatase (ALP) activity and increased the expression of transforming growth factor‑β1, Smad2, Smad3 and Runt‑related transcription factor (Runx)2...
August 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899682/caveolin-1-controls-hyperresponsiveness-to-mechanical-stimuli-and-fibrogenesis-associated-runx2-activation-in-keloid-fibroblasts
#9
Chao-Kai Hsu, Hsi-Hui Lin, Hans I-Chen Harn, Rei Ogawa, Yang-Kao Wang, Yen-Ting Ho, Wan-Rung Chen, Yi-Chao Lee, Julia Yu-Yun Lee, Shyh-Jou Shieh, Chao-Min Cheng, John A McGrath, Ming-Jer Tang
Keloids are pathological scars characterized by excessive extracellular matrix production that are prone to form in body sites with increased skin tension. Caveolin-1 (CAV1), the principal coat protein of caveolae, has been associated with the regulation of cell mechanics, including cell softening and loss of stiffness sensing ability in NIH3T3 fibroblasts. Although CAV1 is present in low amounts in keloid fibroblasts (KFs), the causal association between CAV1 downregulation and its aberrant responses to mechanical stimuli remain unclear...
September 9, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28899668/the-sp7-gene-is-required-for-maturation-of-osteoblast-linage-cells-in-medaka-oryzias-latipes-vertebral-column-development
#10
Yuki Azetsu, Keiji Inohaya, Yoshiro Takano, Masato Kinoshita, Mai Tasaki, Akira Kudo
Sp7 is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, we established medaka sp7 mutants using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures. In particular, the neural and the hemal arches were not formed, although the chordal centra were formed. Analysis of the transgenic medaka revealed that sp7 mutant had normal distribution of type X collagen a1 a (col10a1a)-positive osteoblast-like cells around the centrum and at the proximal region of the vertebral arch...
September 9, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28898414/bmp-signaling-regulates-the-skeletal-and-connective-tissue-differentiation-during-caudal-fin-regeneration-in-sailfin-molly-poecilia-latipinna
#11
Shailja Rajaram, Sonam Patel, Gowri Kumari Uggini, Isha Desai, Suresh Balakrishnan
Caudal fin regeneration in sailfin molly, Poecilia latipinna (Lesueur 1821) involves an initial wound healing stage, followed by blastema that is formed of fast proliferating cells. In order to replicate the lost fin, correct differentiation of the blastemal cells into various tissues is the prime essence. Among the molecular signals governing proper differentiation of blastemal cells, members of the bone morphogenetic protein (BMP) family are crucial. Herein, we investigated the specific effects of inhibition of BMP signaling using LDN193189 on skeletal and connective tissue formation in the regenerating tail fin of P...
September 12, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28898321/-cleidocranial-dysplasia-a-case-report
#12
Olga Medina, Nelson Muñoz, Carlos Moneriz
INTRODUCTION: Cleidocraneal dysplasia (CCD) is a rare skeletal autosomal dominant syndrome characterized by dental anomalies and bone abnormalities. These clinical manifestations do not require treatment in most cases. The disease is caused by mutation in the gene RUNX2 (CBAF1), located on the short arm of chromosome 6. OBJECTIVE: To report a case of CCD and perform a literature review focused on clinical manifestations and diagnosis. CASE REPORT: A 3 year old patient, who was clinically diagnosed with CCD since birth...
2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/28895234/runx2-induction-during-differentiation-of-wharton-s-jelly-mesenchymal-stem-cells-to-osteoblasts-is-regulated-by-jarid1b-histone-demethylase
#13
Francisco Bustos, Hugo Sepúlveda, Catalina P Prieto, Margarita Carrasco, Lorena Díaz, José Palma, José Lattus, Martín Montecino, Verónica Palma
Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSC) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the RUNX2 osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing JARID1B histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential...
September 12, 2017: Stem Cells
https://www.readbyqxmd.com/read/28889456/harmine-promotes-periodontal-ligament-cell-induced-tissue-regeneration
#14
Hyun-Chang Lim, Byung-Yoon Cha, Sun U Song, Jeong-Ho Yun
OBJECTIVE: to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)-induced tissue regeneration. MATERIALS AND METHODS: Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP-2 and hPDLC/harmine by alizarin red S staining, real-time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model...
September 10, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28887210/can-half-marathon-affect-overall-health-the-yin-yang-of-sport
#15
Luca Dalle Carbonare, Marcello Manfredi, Giuseppe Caviglia, Eleonora Conte, Elisa Robotti, Emilio Marengo, Samuele Cheri, Francesco Zamboni, Daniele Gabbiani, Michela Deiana, Daniela Cecconi, Federico Schena, Monica Mottes, Maria Teresa Valenti
Physical activity improves overall health and counteracts metabolic pathologies. Adipose tissue and bone are important key targets of exercise; the prevalence of diseases associated with suboptimal physical activity levels has increased in recent times as a result of lifestyle changes. Mesenchymal stem cells (MSCs) differentiation in either osteogenic or adipogenic lineage is regulated by many factors. Particularly, the expression of master genes such as RUNX2 and PPARγ2 is essential for MSC commitment to osteogenic or adipogenic differentiation, respectively...
September 5, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28882757/ion-channel-functional-protein-kinase-trpm7-regulates-mg-ions-to-promote-the-osteoinduction-of-human-osteoblast-via-pi3k-pathway-in-vitro-simulation-of-the-bone-repairing-effect-of-mg-based-alloy-implant
#16
Xiuzhi Zhang, Haiyue Zu, Dewei Zhao, Ke Yang, Simiao Tian, Xiaoming Yu, Faqiang Lu, Baoyi Liu, Xiaobing Yu, Benjie Wang, Wei Wang, Shibo Huang, Yongxuan Wang, Zihua Wang, Zhaodong Zhang
Mg-based alloys, as the potential orthopaedic implant, can self-degrade to avoid second operation for its remove, and enable to promote bone repair; however, the underlying molecular mechanisms remain unclear. In the present study, we examined the effect of Mg ions on osteogenesis, chemotaxis and anti-alkaline stress in hFOB1.19 human osteoblast cells to simulate bone-repairing effect of a biodegradable Mg-based alloy implant in vitro, and explored the regulatory role of the transient receptor potential melastatin 7 (TRPM7)/phosphoinositide 3-kinase (PI3K) signalling pathway in the process of Mg ion-induced bone repair by knockdown of TRPM7 and antagonizing PI3K activity...
September 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28881301/surface-immobilization-of-gelatin-onto-tio2-nanotubes-to-modulate-osteoblast-behavior
#17
Min Lai, Ziyang Jin, Weili Qiao
To improve the bioactivity of titanium implants, a homogeneous layer of TiO2 nanotubes with a diameter of approximately 110nm was prepared by anodization. Gelatin was immobilized onto TiO2 nanotubes through an intermediate layer of polydopamine. The surface characteristics of different substrates were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and contact angle measurements, respectively. These results demonstrate that gelatin was successfully immobilized onto TiO2 nanotubes...
August 26, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28879758/light-controlled-bmsc-sheet-implant-complexes-with-improved-osteogenesis-via-an-lrp5-%C3%AE-catenin-runx2-regulatory-loop
#18
Zhiwei Jiang, Huiming Wang, Ke Yu, Yuting Feng, Ying Wang, Tingben Huang, Kaichen Lai, Yue Xi, Guoli Yang
The combination of bone marrow mesenchymal stem cell (BMSC) sheets and titanium implants (BMSC sheet-implant complexes) can accelerate osseointegration. However, methods of fabricating BMSC sheet-implant complexes are quite limited, and the survival of BMSC sheet-implant complexes is one of the key barriers. Here, we show that a light-controlled fabricating system can generate less injured BMSC sheet-implant complexes with improved viability and osteogenesis, and non-invasive monitoring of viability of BMSC sheet-implant complexes using lentiviral delivery system is feasible...
September 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28879048/no-evidence-for-a-direct-role-of-hla-b27-in-pathological-bone-formation-in-axial-spa
#19
Barbara Neerinckx, Simon Kollnberger, Jacqueline Shaw, Rik Lories
OBJECTIVE: The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis and osteogenesis. METHODS: Different in vitro differentiation systems were used to mimic endochondral and direct bone formation. ATDC5 cells and primary human periosteum-derived cells (hPDCs) were transduced with lentiviral vectors expressing HLA-B27 or HLA-B7...
2017: RMD Open
https://www.readbyqxmd.com/read/28878609/novel-mutation-of-the-runx2-gene-in-patients-with-cleidocranial-dysplasia
#20
Ewa Hordyjewska, Anna Jaruga, Grzegorz Kandzierski, Przemko Tylzanowski
Cleidocranial dysplasia (CCD) is an autosomal dominant disorder linked to mutations in the Runt-related transcription factor 2, encoded by the RUNX2 gene, which is essential for osteoblast differentiation and skeletal development. Here, we describe a novel nonsense mutation (c.532C>T; p.Q178X) in RUNX2 identified in 3 affected members of a Polish family with CCD. The localization and transcriptional transactivation studies show that the mutated form of the protein has altered the subcellular localization and significantly decreased transactivation properties, respectively...
August 2017: Molecular Syndromology
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