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https://www.readbyqxmd.com/read/29140298/enhancement-of-bone-marrow-derived-mesenchymal-stem-cell-osteogenesis-and-new-bone-formation-in-rats-by-obtusilactone-a
#1
Yi-Hsiung Lin, Chung-Yi Chen, Liang-Yin Chou, Chung-Hwan Chen, Lin Kang, Chau-Zen Wang
The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias...
November 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29138823/mechanical-strain-promotes-osteogenic-differentiation-of-bone-mesenchymal-stem-cells-from-ovariectomized-rats-via-the-phosphoinositide-3%C3%A2-kinase-akt-signaling-pathway
#2
Ningjuan Ouyang, Peng Zhang, Runqing Fu, Guofang Shen, Lingyong Jiang, Bing Fang
Osteoporosis has become an overwhelming public health problem worldwide. As an elementary physiological factor to regulate bone formation and regeneration, mechanical strain may be used as a non‑invasive intervention in osteoporosis prevention and treatment. However, little is known regarding the underlying mechanism. The aim of the current study was to investigate the effect of continuous mechanical strain (CMS) on osteogenic differentiation of bone mesenchymal stem cells (BMSCs) from ovariectomized rats (OVX BMSCs)...
November 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138637/regulation-of-osteogenic-differentiation-of-placental-derived-mesenchymal-stem-cells-by-insulin-like-growth-factors-and-low-oxygen-tension
#3
Amer Youssef, Victor K M Han
Placental mesenchymal stem cells (PMSCs) are multipotent cells that can differentiate in vitro to multiple lineages, including bone. Insulin-like growth factors (IGFs, IGF-1 and IGF-2) participate in maintaining growth, survival, and differentiation of many stem cells, including osteoprogenitors. Low oxygen tension (PO2) can maintain stem cell multipotency and impede osteogenic differentiation. In this study, we investigated whether PMSC osteogenic differentiation is influenced by low PO2 and by IGFs. Our results indicated that low PO2 decreased osteogenic markers RUNX2 and OPN; however, re-exposure to higher oxygen tension (room air) restored differentiation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29137952/neural-egfl-like-1-regulates-cartilage-maturation-through-runt-related-transcription-factor-3-mediated-indian-hedgehog-signaling
#4
Chenshuang Li, Zhong Zheng, Jie Jiang, Wenlu Jiang, Kevin Lee, Emily A Berthiaume, Eric C Chen, Cymbeline T Culiat, Yan-Heng Zhou, Xinli Zhang, Kang Ting, Chia Soo
The pro-chondrogenic function of runt-related transcription factor 2 (Runx2) was previously considered to be dependent on direct binding with the promoter of Indian hedgehog (Ihh)-the major regulator of chondrocyte differentiation, proliferation, and maturation. Our previous studies identified neural EGFL like 1 (Nell-1) as a Runx2-responsive growth factor for chondrogenic differentiation and maturation. In this study, we further revealed that the pro-chondrogenic activities of Nell-1 also rely on Ihh signaling, by showing: [1] Nell-1 significantly elevated Ihh signal transduction, [2] Nell-1 deficiency markedly reduced Ihh activation in chondrocytes, and [3] Nell-1-stimulated chondrogenesis was significantly reduced by the specific hedgehog inhibitor, cyclopamine...
November 11, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29136173/high-levels-of-gsk-3%C3%AE-signaling-reduce-osteogenic-differentiation-of-stem-cells-in-osteonecrosis-of-femoral-head
#5
Lu Huang, Ying Wang, Yangzi Jiang, Yan Wu, Changchang Hu, Hongwei Ouyang
Osteonecrosis of the femoral head (ONFH) is a common but intractable disease. In this study, we investigated the mechanisms regulating alterations in mesenchymal stem cell (MSC) differentiation in ONFH. Five patients who were diagnosed with ONFH were enrolled in this study. BMSCs were isolated from the osteonecrotic zone in the femoral head (FH-pMSCs) and from the normal zone in the pelvis (hMSCs) of the same patient. Morphology, cell proliferation, and expression of mediators of the Wnt signaling pathway were evaluated...
November 10, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29132091/effect-of-anti-diabetic-drugs-on-bone-metabolism-evidence-from-preclinical-and-clinical-studies
#6
REVIEW
Mohammad Adil, Rashid Ali Khan, Abul Kalam, Shiva Kumar Venkata, Amit Dattatraya Kandhare, Pinaki Ghosh, Manju Sharma
Diabetes mellitus is associated with abnormal bone health and an increased risk of fracture even though patients have normal or higher BMD. The mechanisms behind diabetes mellitus- induced various skeletal disorders remain unclear. Anti-diabetic drugs may have negative or positive impact on bone metabolism. For instance, thiazolidinediones increases the bone loss and risk of fracture possibly through PPARγ activation in bone marrow cells and hamper osteoblastogenesis via decreasing Runx2 transcription factor, IGF-1 and Wnt signalling pathways...
May 25, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29130990/increased-bone-morphogenetic-protein-signaling-in-the-cutaneous-vasculature-of-patients-with-calciphylaxis
#7
Sagar U Nigwekar, Pawina Jiramongkolchai, Florian Wunderer, Emily Bloch, Rika Ichinose, Rosalynn M Nazarian, Ravi I Thadhani, Rajeev Malhotra, Donald B Bloch
BACKGROUND: The objective of this study was to investigate the role of bone morphogenetic protein (BMP) signal transduction in the pathogenesis of calciphylaxis. METHODS: Skin biopsy specimens were obtained from 18 patients with, and 12 patients without, calciphylaxis. Tissue sections were stained with antibodies directed against BMP effector proteins phosphorylated-SMAD (p-SMAD) 1/5/9, inhibitor of DNA 1 (Id1), inhibitor of DNA 3 (Id3), and Runx2. The intensity of staining was scored semi-quantitatively as strong versus weak or absent...
November 9, 2017: American Journal of Nephrology
https://www.readbyqxmd.com/read/29129496/runt-related-transcription-factor-2-runx2-inhibits-apoptosis-of-intestinal-epithelial-cells-in-crohn-s-disease
#8
Liugen Gu, Juan Zhao, Shiqing Zhang, Weisong Xu, Runzhou Ni, Xiaojuan Liu
Apoptosis in intestinal epithelial cells (IECs) prevents the development of Crohn's disease (CD), a type of inflammatory bowel disease (IBD). Runt-related transcription factor 2 (Runx2) inhibits apoptosis in osteosarcoma-derived U2OS cells via down-regulating the transcriptional activity of p53. However, the expression and function of Runx2 in CD remain unclear. In this study, Runx2 protein levels were decreased in the intestinal epithelial cells (IECs) of CD patients and in a mouse 2, 4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis model; in contrast, the expression levels of p53 and Bax, a p53-target gene, were increased...
November 8, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29126771/bmp-2-plasmid-dna-loaded-chitosan-films-a-new-strategy-for-bone-engineering
#9
Juan Li, Jun Lin, Wenke Yu, Xiaojia Song, Qiaoling Hu, Jing-Hong Xu, Huiming Wang, Christian Mehl
OBJECTIVES: Bone defects are common in every area of medicine and remain a clinical challenge. Tissue engineering has led to promising new strategies in accelerating bone repair. Bone morphogenetic proteins (BMPs) play crucial roles in bone regeneration, but are required in supra-physiological doses, which are expensive and produce severe side effects. METHODS: To address these issues, we prepared BMP-2 plasmid DNA-loaded chitosan films, and examined their effects on mouse osteoblast-like MC3T3-E1 cell morphology, proliferation, and runt-related transcription factor 2 (RUNX2) expression...
October 14, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29126229/osteoblast-amp-activated-protein-kinase-regulates-postnatal-skeletal-development-in-male-mice
#10
Ippei Kanazawa, Ayumu Takeno, Ken-Ichiro Tanaka, Masakazu Notsu, Toshitsugu Sugimoto
Studies have shown that AMP-activated protein kinase (AMPK), a crucial regulator of energy homeostasis, plays important roles in osteoblast differentiation and mineralization. However, little is known about in vivo roles of osteoblastic AMPK in bone development. Thus, to investigate in vivo roles of osteoblast AMPK, we conditionally inactivated Ampk in osterix (Osx)-expressing cells by crossing Osx-Cre mice with floxed AMPKα1 to generate mice lacking AMPKα1 in osteoblasts (Ampk-/- mice). Compared with wild-type and Ampk+/- mice, Ampk-/- mice displayed retardation of postnatal bone development, although bone deformity was not observed at birth...
November 3, 2017: Endocrinology
https://www.readbyqxmd.com/read/29124284/ca-2-nuclear-factor-of-activated-t-cells-signaling-is-enriched-in-early-onset-rectal-tumors-devoid-of-canonical-wnt-activation
#11
Raju Kumar, Ratheesh Raman, Viswakalyan Kotapalli, Swarnalata Gowrishankar, Saumyadipta Pyne, Jonathan R Pollack, Murali D Bashyam
Our previous extensive analysis revealed a significant proportion of early-onset colorectal tumors from India to be localized to the rectum in younger individuals and devoid of deregulated Wnt/β-catenin signaling. In the current study, we performed a comprehensive genome-wide analysis of clinically well-annotated microsatellite stable early-onset sporadic rectal cancer (EOSRC) samples. Results revealed extensive DNA copy number alterations in rectal tumors in the absence of deregulated Wnt/β-catenin signaling...
November 9, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29122669/osteoanabolic-activity-of-whey-derived-anti-oxidative-mhirl-and-yveel-and-angiotensin-converting-enzyme-inhibitory-yllf-alpmhir-ipa-and-wlahk-bioactive-peptides
#12
Masum Pandey, Rajeev Kapila, Suman Kapila
Exploring bone rebuilding anabolic agents has been gaining much attention due to their potential therapeutic effects in treating several bone disorders including osteoporosis. Whey protein has been reported to affect bone health osteoanabolically, in terms of proliferation and differentiation of primary osteoblast cells. This study investigates whether whey derived anti-oxidative (AO) (P1- MHIRL, P2- YVEEL) and angiotensin converting enzyme inhibitory (ACE inhibitory) (P3- YLLF, P4-ALPMHIR, P5-IPA, P6- WLAHK) bioactive peptides affect the proliferation and differentiation of primary osteoblast cells isolated from rat calvaria...
November 6, 2017: Peptides
https://www.readbyqxmd.com/read/29115171/sika-pilose-antler-type-i-collagen-promotes-bmsc-differentiation-via-the-erk1-2-and-p38-mapk-signal-pathways
#13
Yanshuang Wang, Su Luo, Dafang Zhang, Xiaobo Qu, Yinfeng Tan
CONTEXT: Sika pilose antler type I collagen (SPC-I) have been reported to promote bone marrow mesenchymal stem cell (BMSC) proliferation and differentiation. However, the underlying mechanism is still unclear. OBJECTIVE: This study investigates the molecular mechanisms of SPC-I on the BMSC proliferation and differentiation of osteoblast (OB) in vitro. MATERIAL AND METHODS: The primary rat BMSC was cultured and exposed to SPC-I at different concentrations (2...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29111170/moderate-intensity-4mt-static-magnetic-fields-prevent-bone-architectural-deterioration-and-strength-reduction-by-stimulating-bone-formation-in-streptozotocin-treated-diabetic-rats
#14
Hao Zhang, Lu Gan, Xiaoquan Zhu, Jun Wang, Licun Han, Peng Cheng, Da Jing, Xiaodong Zhang, Qingshun Shan
Type 1 diabetes mellitus (T1DM) has been associated with deterioration of bone microarchitecture and strength, resulting in increased fracture risk. Substantial studies have revealed the capacity of moderate-intensity static magnetic fields (SMF) on promoting osteoblastogenesis in vitro and stimulating bone growth and bone regeneration in vivo, whereas it is unknown whether SMF can resist T1DM-associated osteopenia/osteoporosis. We herein investigated the potential effects of whole-body SMF exposure with 4mT on bone loss in streptozotocin-induced T1DM rats...
October 27, 2017: Bone
https://www.readbyqxmd.com/read/29107124/genetic-inhibition-of-ppar%C3%AE-s112-phosphorylation-reduces-bone-formation-and-stimulates-marrow-adipogenesis
#15
Chunxi Ge, Guisheng Zhao, BinBin Li, Yan Li, William P Cawthorn, Ormond A MacDougald, Renny T Franceschi
A common feature of many skeletal diseases is the accumulation of marrow fat. A reciprocal relationship exists between osteogenesis and adipogenesis in bone marrow that is mediated by the relative activity of PPARγ and RUNX2 transcription factors. The ERK/MAPK pathway is an important inducer of MSC differentiation to osteoblasts and an inhibitor of adipogenesis that functions by phosphorylating RUNX2 and PPARγ. To begin to assess the importance of this regulation in vivo, we examined the consequences of blocking one arm of this pathway, PPARγ S112 phosphorylation, by evaluating the bone phenotype of PPARγ S112A mutant mice...
October 26, 2017: Bone
https://www.readbyqxmd.com/read/29107070/inflammation-driven-colon-neoplasmatogenesis-in-upa-deficient-mice-is-associated-with-an-increased-expression-of-runx-transcriptional-regulators
#16
Hara Afaloniati, George S Karagiannis, Alexandros Hardas, Theofilos Poutahidis, Katerina Angelopoulou
Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of inflammation-driven intestinal neoplasmatogenesis, in which chronic colitis and CRC are induced using dextran sodium sulfate (DSS). In this report, we explored the contribution of intracellular components of Smad-mediated BMP signal transduction using the same model...
October 26, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29106055/lnc-ntf3-5-promotes-osteogenic-differentiation-of-maxillary-sinus-membrane-stem-cells-via-sponging-mir-93-3p
#17
Wei Peng, Shuang-Xi Zhu, Jin Wang, Li-Li Chen, Jun-Quan Weng, Song-Ling Chen
BACKGROUND: The function and the mechanism of long non-coding RNAs (lncRNAs) on the osteogenic differentiation of maxillary sinus membrane stem cells (MSMSCs) remain largely unknown. MATERIALS AND METHODS: The expression of lnc-NTF3-5 and Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and Alkaline Phosphatase (ALP) was examined by quantitative real-time PCR (qRT-PCR) in MSMSCs during the process osteogenic differentiation. Then the function of lnc-NTF3-5 was evaluated by loss- and gain-of-function techniques, as well as qRT-PCR, western blot, and Alizarin Red staining...
November 6, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/29098245/-osteogenic-development-in-vivo-and-osteogenic-differentiation-in-vitro-of-mouse-maxillary-primordium-mesenchymal-cells-a-comparative-study
#18
Jun Zheng, Lei Wang, Dong-Liang Mu, Yu-Fang DU, Li-Jia Liu, Jie Zheng
PURPOSE: To compare the differences between the osteogenic development in vivo and osteogenic differentiation in vitro of mouse maxillary primordium mesenchymal cells (MPMCs). METHODS: E10.5 and E17.5 mouse MPMCs were cultured in vitro to observe cell morphology. E10.5 primary MPMCs, after culturing in vitro for 3 days, were cultured in osteogenic differentiation in vitro for another 7 days. Then immunofluorescence and qPCR were used to compare the difference of osteogenic differentiation with E17...
June 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29095952/computer-simulation-models-as-a-tool-to-investigate-the-role-of-micrornas-in-osteoarthritis
#19
Carole J Proctor, Graham R Smith
The aim of this study was to show how computational models can be used to increase our understanding of the role of microRNAs in osteoarthritis (OA) using miR-140 as an example. Bioinformatics analysis and experimental results from the literature were used to create and calibrate models of gene regulatory networks in OA involving miR-140 along with key regulators such as NF-κB, SMAD3, and RUNX2. The individual models were created with the modelling standard, Systems Biology Markup Language, and integrated to examine the overall effect of miR-140 on cartilage homeostasis...
2017: PloS One
https://www.readbyqxmd.com/read/29094227/down-regulation-of-noggin-and-mir-138-coordinately-promote-osteogenesis-of-mesenchymal-stem-cells
#20
Xing-Kun Sun, Jin Zhou, Lei Zhang, Tian Ma, Yu-Han Wang, Yan-Mei Yang, Yan-Ting Tang, Hong Li, Li-Jun Wang
Mesenchymal stem cells (MSCs) can differentiate to osteocytes under suitable conditions. In recent years, micro-nucleotides have been progressively used to modulate gene expression in cells due to the consideration of safety. Our present study aimed to investigate whether co-delivery of Noggin-siRNA and antimiR-138 enhances the osteogenic effect of MSCs. Using a murine MSC line, C3H/10T1/2 cells, the delivery efficiency of Noggin-siRNA and antimiR-138 into MSCs was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR)...
November 2, 2017: Journal of Molecular Histology
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