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https://www.readbyqxmd.com/read/28531552/the-role-of-next-generation-sequencing-in-infection-prevention-in-human-parainfluenza-virus-3-infections-in-immunocompromised-patients
#1
Atul Kothari, Mary J Burgess, Juan Carlos Rico Crescencio, Joshua L Kennedy, Jesse L Denson, Kurt C Schwalm, Ashley N Stoner, John C Kincaid, Faith E Davies, Darrell L Dinwiddie
BACKGROUND: Respiratory viral infections are a significant problem in patients with hematologic malignancies. We report a cluster of HPIV 3 infections in our myeloma patients, and describe the utility of next generation sequencing (NGS) to identify transmission linkages which can assist in infection prevention. OBJECTIVES: To evaluate the utility of NGS to track respiratory viral infection outbreaks and delineate between community acquired and nosocomial infections in our cancer units...
May 12, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28529841/rapid-and-conformal-coating-of-polymer-resins-by-airbrushing-for-continuous-and-high-speed-roll-to-roll-nanopatterning-parametric-quality-controls-and-extended-applications
#2
Jae Hyuk Lee, Minho Na, Jiyeop Kim, Kangeun Yoo, Jaekyu Park, Jeong Dae Kim, Dong Kyo Oh, Seungjo Lee, Hongseok Youn, Moon Kyu Kwak, Jong G Ok
We present a facile and scalable coating method based on controlled airbrushing, which is suitable for conformal resin coating in continuous roll-to-roll (R2R) nanoimprint lithography (NIL) process. By controlling the concentration of UV-curable polymeric resin with mixing the volatile solvent and its airbrushing time, the coated resin film thickness can be readily tuned. After R2R NIL using a flexible nanoscale line pattern (nanograting) mold is conducted upon the airbrushed resin film, a large-area uniform nanograting pattern is fabricated with controlled residual layer thickness (RLT) based on the initial film thickness...
2017: Nano Convergence
https://www.readbyqxmd.com/read/28528441/herpesvirus-latency-on-the-importance-of-positioning-oneself
#3
Patrick Lomonte
The nucleus is composed of multiple compartments and domains, which directly or indirectly influence many cellular processes including gene expression, RNA splicing and maturation, protein post-translational modifications, and chromosome segregation. Nuclear-replicating viruses, especially herpesviruses, have co-evolved with the cell, adopting strategies to counteract and eventually hijack this hostile environment for their own benefit. This allows them to persist in the host for the entire life of an individual and to ensure their maintenance in the target species...
2017: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/28526672/a-systematic-review-and-meta-analysis-of-e-mental-health-interventions-to-treat-symptoms-of-posttraumatic-stress
#4
Sara Simblett, Jennifer Birch, Faith Matcham, Lidia Yaguez, Robin Morris
BACKGROUND: Posttraumatic stress disorder (PTSD) is a stress disorder characterized by unwanted intrusive re-experiencing of an acutely distressing, often life-threatening, event, combined with symptoms of hyperarousal, avoidance, as well as negative thoughts and feelings. Evidence-based psychological interventions have been developed to treat these symptoms and reduce distress, the majority of which were designed to be delivered face-to-face with trained therapists. However, new developments in the use of technology to supplement and extend health care have led to the creation of e-Mental Health interventions...
May 17, 2017: JMIR Mental Health
https://www.readbyqxmd.com/read/28526136/emerging-mechanisms-and-roles-for-asymmetric-cytokinesis
#5
C Thieleke-Matos, D S Osório, A X Carvalho, E Morais-de-Sá
Cytokinesis completes cell division by physically separating the contents of the mother cell between the two daughter cells. This event requires the highly coordinated reorganization of the cytoskeleton within a precise window of time to ensure faithful genomic segregation. In addition, recent progress in the field highlighted the importance of cytokinesis in providing particularly important cues in the context of multicellular tissues. The organization of the cytokinetic machinery and the asymmetric localization or inheritance of the midbody remnants is critical to define the spatial distribution of mechanical and biochemical signals...
2017: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/28525579/modulation-of-cyclobutane-thymine-photodimer-formation-in-t11-tracts-in-rotationally-phased-nucleosome-core-particles-and-dna-minicircles
#6
Kesai Wang, John-Stephen A Taylor
Cyclobutane pyrimidine dimers (CPDs) are DNA photoproducts linked to skin cancer, whose mutagenicity depends in part on their frequency of formation and deamination. Nucleosomes modulate CPD formation, favoring outside facing sites and disfavoring inward facing sites. A similar pattern of CPD formation in protein-free DNA loops suggests that DNA bending causes the modulation in nucleosomes. To systematically study the cause and effect of nucleosome structure on CPD formation and deamination, we have developed a circular permutation synthesis strategy for positioning a target sequence at different superhelix locations (SHLs) across a nucleosome in which the DNA has been rotationally phased with respect to the histone octamer by TG motifs...
May 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28522572/the-prognostic-value-of-the-depth-of-response-in-multiple-myeloma-depends-on-the-time-of-assessment-risk-status-and-molecular-subtype
#7
Carolina Schinke, Antje Hoering, Hongwei Wang, Victoria Carlton, Sharmilan Thanendrarajan, Shayu Deshpande, Purvi Patel, Gabor Molnar, Sandra Susanibar, Meera Mohan, Pankaj Mathur, Muthukumar Radhakrishnan, Shadiqul Hoque, Jorge Jo Kamimoto, Monica Grazziutti, Frits van Rhee, Maurizio Zangari, Giovanni Insuasti-Beltran, Daisy Alapat, Ginell Post, Shmuel Yaccoby, Joshua Epstein, Leo Rasche, Sarah Johnson, Martin Moorhead, Tom Willis, Bart Barlogie, Brian Walker, Niels Weinhold, Faith E Davies, Gareth J Morgan
No abstract text is available yet for this article.
May 18, 2017: Haematologica
https://www.readbyqxmd.com/read/28521879/a-quick-simple-method-for-detecting-circulating-fluorescent-advanced-glycation-end-products-correlation-with-in-vitro-and-in-vivo-non-enzymatic-glycation
#8
Marika Villa, Mariacristina Parravano, Arianna Micheli, Lucia Gaddini, Andrea Matteucci, Cinzia Mallozzi, Francesco Facchiano, Fiorella Malchiodi-Albedi, Flavia Pricci
OBJECTIVE: Advanced glycation end-products (AGEs) constitute a highly heterogeneous family of compounds, relevant in the pathogenesis of diabetic complications, which could represent efficient biomarkers of disease progression and drug response. Unfortunately, due to their chemical heterogeneity, no method has been validated to faithfully monitor their levels in the course of the disease. In this study, we refine a procedure to quantitatively analyze fluorescent AGEs (fAGEs), a subset considered remarkably representative of the entire AGE family, and measure them in in vitro glycated BSA (gBSA) and in plasma and vitreous of diabetic rats, for testing its use to possibly quantify circulating AGEs in patients, as markers of metabolic control...
June 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28521599/religious-involvement-and-happiness-assessing-the-mediating-role-of-compassion-and-helping-others
#9
Neal Krause, Gail Ironson, Peter Hill
Research reveals that a number of different aspects of religious involvement are associated with happiness. However, researchers have yet to provide an overarching theoretical explanation for how multiple dimensions of religion might be associated with happiness. The purpose of this study is to develop and test a conceptual model that includes the following core hypotheses: (1) people who attend worship services more often tend to be more committed to their faith; (2) people who are more committed to their faith are more likely to be compassionate; (3) compassionate individuals are more likely to provide emotional support to significant others; and (4) people who provide support to others tend to be happier...
May 19, 2017: Journal of Social Psychology
https://www.readbyqxmd.com/read/28520985/warning-statements-and-safety-practices-among-manufacturers-and-distributors-of-electronic-cigarette-liquids-in-the-united-states
#10
Pebbles Fagan, Pallav Pokhrel, Thaddeus A Herzog, Mignonne C Guy, Kari-Lyn K Sakuma, Dennis R Trinidad, Kevin Cassel, Dorothy Jorgensen, Tania Lynch, Jamie Q Felicitas-Perkins, Sherilyn Palafox, Faith Hamamura, Sarah Maloney, Kaylah Degree, Kymberle Sterling, Eric Moolchan, Mark S Clanton, Thomas Eissenberg
Introduction.: Prior to the Food and Drug Administration's (FDA) regulation of electronic cigarettes and warning statements related to nicotine addiction, there was no critical examination of manufacturer/distributor voluntary practices that could potentially inform FDA actions aimed to protect consumers. This study examined the content of warning statements and safety characteristics of electronic cigarette liquid bottles using a national sample. Methods.: Research staff randomly selected four electronic cigarette liquid manufacturers/distributors from four U...
May 18, 2017: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/28520484/fe-en-acci%C3%A3-n-promoting-physical-activity-among-churchgoing-latinas
#11
Elva M Arredondo, John P Elder, Jessica Haughton, Donald J Slymen, James F Sallis, Lilian G Perez, Natalicio Serrano, Maíra T Parra, Rodrigo Valdivia, Guadalupe X Ayala
OBJECTIVES: To evaluate the impact of a faith-based intervention to promote physical activity in Latinas. METHODS: We randomized 16 churches in San Diego County, California, to a physical activity intervention or cancer screening comparison condition (n = 436). The intervention followed an ecological framework and involved promotoras. We examined 12-month intervention effects, including accelerometer-based moderate-to-vigorous physical activity (MVPA; primary outcome) and secondary outcomes...
May 18, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28517969/prevalence-and-determinants-of-depression-among-patients-under-the-care-of-traditional-health-practitioners-in-a-kenyan-setting-policy-implications
#12
Christine W Musyimi, Victoria N Mutiso, Abednego M Musau, Lydia K Matoke, David M Ndetei
In Kenya, there is paucity of information on depression among traditional health practitioner (THP) patients, particularly in rural areas. The aim of this study was to estimate prevalence and identify determinants of major depressive disorder (MDD) among patients of THP in rural Kenya using the World Health Organization (WHO) Mental Health Gap Action Programme Intervention Guideline (mhGAP-IG). All adult patients seeking care from trained THPs (either traditional healers such as diviners and herbalists or faith healers, who use treatments such as prayers, laying hands on patients, or providing holy water and ash to their patients) over a period of 3 months (September 2014 to November 2014) were screened for depression using mhGAP-IG and their sociodemographic characteristics recorded...
January 1, 2017: Transcultural Psychiatry
https://www.readbyqxmd.com/read/28515443/development-of-a-whole-organism-platform-for-phenotype-based-analysis-of-igf1r-pi3k-akt-tor-action
#13
Chengdong Liu, Wei Dai, Yan Bai, Changfeng Chi, Yi Xin, Gen He, Kangsen Mai, Cunming Duan
Aberrant regulation of the insulin-like growth factor (IGF)/insulin (IIS)-PI3K-AKT-TOR signaling pathway is linked to major human diseases, and key components of this pathway are targets for therapeutic intervention. Current assays are molecular target- or cell culture-based platforms. Due to the great in vivo complexities inherited in this pathway, there is an unmet need for whole organism based assays. Here we report the development of a zebrafish transgenic line, Tg(igfbp5a:GFP), which faithfully reports the mitotic action of IGF1R-PI3K-Akt-Tor signaling in epithelial cells in real-time...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28514649/widespread-mitotic-bookmarking-by-histone-marks-and-transcription-factors-in-pluripotent-stem-cells
#14
Yiyuan Liu, Bobbie Pelham-Webb, Dafne Campigli Di Giammartino, Jiexi Li, Daleum Kim, Katsuhiro Kita, Nestor Saiz, Vidur Garg, Ashley Doane, Paraskevi Giannakakou, Anna-Katerina Hadjantonakis, Olivier Elemento, Effie Apostolou
During mitosis, transcription is halted and many chromatin features are lost, posing a challenge for the continuity of cell identity, particularly in fast cycling stem cells, which constantly balance self-renewal with differentiation. Here we show that, in pluripotent stem cells, certain histone marks and stem cell regulators remain associated with specific genomic regions of mitotic chromatin, a phenomenon known as mitotic bookmarking. Enhancers of stem cell-related genes are bookmarked by both H3K27ac and the master regulators OCT4, SOX2, and KLF4, while promoters of housekeeping genes retain high levels of mitotic H3K27ac in a cell-type invariant manner...
May 16, 2017: Cell Reports
https://www.readbyqxmd.com/read/28514629/the-emergent-properties-of-conspecific-attraction-can-limit-a-species-ability-to-track-environmental-change
#15
Kirk W Stodola, Michael P Ward
Multiple biotic, abiotic, and evolutionary constraints interact to determine a species' range. However, most species are not present in all suitable and accessible locations. Dispersal ability may explain why many species do not occupy all suitable habitat, but highly mobile species also exhibit a mismatch. Habitat selection behavior where individuals are site faithful and settle near conspecifics could create a social pressure that make a species' geographic range resistant to change. We investigated this possibility by using an individual-based model of habitat selection where habitat quality moved each year...
June 2017: American Naturalist
https://www.readbyqxmd.com/read/28512323/nicotine-in-floral-nectar-pharmacologically-influences-bumblebee-learning-of-floral-features
#16
D Baracchi, A Marples, A J Jenkins, A R Leitch, L Chittka
Many plants defend themselves against herbivores by chemical deterrents in their tissues and the presence of such substances in floral nectar means that pollinators often encounter them when foraging. The effect of such substances on the foraging behaviour of pollinators is poorly understood. Using artificial flowers in tightly-controlled laboratory settings, we examined the effects of the alkaloid nicotine on bumblebee foraging performance. We found that bumblebees confronted simultaneously with two equally rewarded nicotine-containing and nicotine-free flower types are deterred only by unnaturally high nicotine concentrations...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28512237/regulation-of-dna-demethylation-by-the-xpc-dna-repair-complex-in-somatic-and-pluripotent-stem-cells
#17
Jaclyn J Ho, Claudia Cattoglio, David T McSwiggen, Robert Tjian, Yick W Fong
Faithful resetting of the epigenetic memory of a somatic cell to a pluripotent state during cellular reprogramming requires DNA methylation to silence somatic gene expression and dynamic DNA demethylation to activate pluripotency gene transcription. The removal of methylated cytosines requires the base excision repair enzyme TDG, but the mechanism by which TDG-dependent DNA demethylation occurs in a rapid and site-specific manner remains unclear. Here we show that the XPC DNA repair complex is a potent accelerator of global and locus-specific DNA demethylation in somatic and pluripotent stem cells...
April 15, 2017: Genes & Development
https://www.readbyqxmd.com/read/28511764/telehealth-wave-surf-s-up-for-fed-state-policymakers
#18
Richard Mark Kirkner
If millions of Americans lose Medicaid or private health insurance coverage because of the unACAing of American health care, telehealth may seem like a gimmicky sideshow rather than a good-faith effort to bring health care into the digital century.
April 2017: Managed Care
https://www.readbyqxmd.com/read/28511602/evaluation-of-student-handwashing-practices-during-a-school-based-hygiene-program-in-rural-western-kenya-2007
#19
Genevieve La Con, Katharine Schilling, Julie Harris, Bobbie Person, Mercy Owuor, Lorraine Ogange, Sitnah Faith, Robert Quick
Unsafe drinking water and inadequate handwashing facilities in primary schools increase the risk of absenteeism due to diarrhea and respiratory infections. To mitigate these risks, we provided 28 schools in rural Western Kenya with handwashing and drinking water stations (containers with lids and taps on metal stands), bleach for water treatment, soap for handwashing, and educational materials. We observed the use of the water stations and assessed teachers' attitudes toward the intervention. Of 151 total handwashing stations, 69 (59%) were observed to have soap and water and treated drinking water 4 months after implementation; observations of pupils showed an increase in handwashing behavior in water stations located < 10 m, as compared with those >10 m, from latrines ( p < ...
January 2017: International Quarterly of Community Health Education
https://www.readbyqxmd.com/read/28510289/genome-organization-during-the-cell-cycle-unity-in-division
#20
REVIEW
Rosela Golloshi, Jacob T Sanders, Rachel Patton McCord
During the cell cycle, the genome must undergo dramatic changes in structure, from a decondensed, yet highly organized interphase structure to a condensed, generic mitotic chromosome and then back again. For faithful cell division, the genome must be replicated and chromosomes and sister chromatids physically segregated from one another. Throughout these processes, there is feedback and tension between the information-storing role and the physical properties of chromosomes. With a combination of recent techniques in fluorescence microscopy, chromosome conformation capture (Hi-C), biophysical experiments, and computational modeling, we can now attribute mechanisms to many long-observed features of chromosome structure changes during cell division...
May 16, 2017: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
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