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oncorhynchus mykiss

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https://www.readbyqxmd.com/read/29792547/a-method-to-quantify-genotoxicity-of-malathion-in-rainbow-trout-using-the-weighted-averaging
#1
Hadi Poorbagher, Hamed Ghaffari Farsani, Hamid Farahmand
DNA breakage has been frequently used as a biomarker of the pesticide toxicity. The present study introduced a method to quantify the DNA breakage in Oncorhynchus mykiss exposed to the pesticide Malathion. Specimens were exposed to different concentrations of malathion for 1-9 days and their gill and liver were sampled. DNA was extracted and electrophoresed using agarose gel. The pixel density curves were obtained from the gel smears. The area under the curves were arbitrarily divided from 3 up to 7 segments using a Java macro in the software ImageJ...
May 24, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29777415/preparation-of-biologically-active-monomeric-recombinant-zebrafish-danio-rerio-and-rainbow-trout-oncorhynchus-mykiss-recombinant-growth-hormones
#2
Ewa Ocłoń, Gili Solomon, Zvi Hayouka, Arieh Gertler
Fish growth hormones (GHs) play an important role in regulating growth, metabolism, reproduction, osmoregulation, and immunity and have thus garnered attention for their application in aquaculture. Zebrafish GH (zGH) cDNA or rainbow trout GH (rtGH) cDNA was cloned into the pMon3401 vector, expressed in MON105-competent Escherichia coli and purified to homogeneity. Their biological activity was evidenced by their ability to interact with ovine GH receptor extracellular domain and stimulate GH receptor-mediated proliferation in FDC-P1-3B9 cells stably transfected with rabbit GH receptor...
May 18, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29776922/core-vs-diet-associated-and-postprandial-bacterial-communities-of-the-rainbow-trout-oncorhynchus-mykiss-midgut-and-feaces
#3
Eleni Mente, Eleni Nikouli, Efthimia Antonopoulou, Samuel A M Martin, Konstantinos Ar Kormas
This study investigated the impact of different dietary ingredients with different protein/lipid sources on the midgut and feaces bacteria communities structure just before feeding and 3h after feeding a single meal on rainbow trout ( Oncorhynchus mykiss ) individuals. Fish were kept in experimental rearing facilities and fed ad libitum two times daily for five weeks. Fish were fed three different commercial diets containing a higher marine fishmeal/fish oil and a lower marine fishmeal/fish oil content. DNA was extracted from midgut and faeces samples for the analysis of their bacterial 16S rRNA gene diversity by targeting the V3-V4 region with 454 pyrosequencing...
May 18, 2018: Biology Open
https://www.readbyqxmd.com/read/29773635/draft-genome-sequence-of-the-fish-pathogen-flavobacterium-columnare-strain-ms-fc-4
#4
Ryan P Bartelme, Paul Barbier, Ryan S Lipscomb, Scott E LaPatra, Ryan J Newton, Jason P Evenhuis, Mark J McBride
Flavobacterium columnare MS-FC-4 is a highly virulent genetic group 1 (formerly genomovar I) strain isolated from rainbow trout ( Oncorhynchus mykiss ). The draft genome consists of three contigs totaling 3,449,277 bp with 2,811 predicted open reading frames. F. columnare MS-FC-4 is a model strain for functional genomic analyses.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29770134/comparative-immune-and-stress-related-transcript-response-induced-by-air-exposure-and-vibrio-anguillarum-bacterin-in-rainbow-trout-oncorhynchus-mykiss-and-gilthead-seabream-sparus-aurata-mucosal-surfaces
#5
Ali Reza Khansari, Joan Carles Balasch, Eva Vallejos-Vidal, David Parra, Felipe E Reyes-López, Lluís Tort
Fish have to face various environmental challenges that may compromise the efficacy of the immune response in mucosal surfaces. Since the effect of acute stress on mucosal barriers in fish has still not been fully elucidated, we aimed to compare the short-term mucosal stress and immune transcriptomic responses in a freshwater (rainbow trout, Oncorhynchus mykiss ) and a marine fish (gilthead seabream, Sparus aurata ) to bacterial immersion ( Vibrio anguillarum bacterin vaccine) and air exposure stress in skin, gills, and intestine...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29756177/interspecific-germ-cell-transplantation-a-new-light-in-the-conservation-of-valuable-balkan-trout-genetic-resources
#6
Jelena Lujić, Zoran Marinović, Simona Sušnik Bajec, Ida Djurdjevič, Béla Urbányi, Ákos Horváth
Interspecific transplantation of germ cells from the brown trout Salmo trutta m. fario and the European grayling Thymallus thymallus into rainbow trout Oncorhynchus mykiss recipients was carried out in order to improve current practices in conservation of genetic resources of endangered salmonid species in the Balkan Peninsula. Current conservation methods mainly include in situ efforts such as the maintenance of purebred individuals in isolated streams and restocking with purebred fingerlings; however, additional ex situ strategies such as surrogate production are needed...
May 13, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29751256/peer-pressure-on-the-riverbank-assessing-catch-and-release-anglers-willingness-to-sanction-others-bad-behavior
#7
Meaghan L Guckian, Andy J Danylchuk, Steven J Cooke, Ezra M Markowitz
Given the well-documented impacts of angler behavior on the biological fitness of angled and released fish, optimizing the conservation value of catch-and-release angling hinges on the extent to which anglers are willing to adopt recommended best practices and refrain from harmful ones. One potentially powerful mechanism underlying adoption of best practices is the social pressure anglers can apply to one another to enforce community norms and values. Past work in other domains demonstrates that forms of interpersonal communication-including social sanctioning-can foster context-appropriate social norms and increase cooperative behavior; yet to date, little research has examined these dynamics in the context of species conservation...
May 7, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29747051/immune-modulation-of-two-batf3-paralogues-in-rainbow-trout-oncorhynchus-mykiss
#8
Jun Wang, Min Liu, Yang Wu, Sohye Yoon, Abdo Alnabulsi, Fuguo Liu, Clara Fernández-Álvarez, Tiehui Wang, Jason W Holland, Chris J Secombes, Jun Zou
Basic leucine zipper transcription factor ATF-like (BATF) -3 is a member of the activator protein 1 (AP‑1) family of transcription factors and is known to play a vital role in regulating differentiation of antigen-presenting cells in mammals. In this study, two BATF3 homologues (termed BATF3a and BATF3b) have been identified in rainbow trout (Oncorhynchus mykiss). Both genes were constitutively expressed in tissues, with particularly high levels of BATF3a in spleen, liver, pyloric caecae and head kidney. BATF3a was also more highly induced by PAMPs and cytokines in cultured cells, with type II IFN a particularly potent inducer...
May 7, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29741132/pumpkin-seed-cake-as-a-fishmeal-substitute-in-fish-nutrition-effects-on-growth-performance-morphological-traits-and-fillet-colour-of-two-freshwater-salmonids-and-two-catfish-species
#9
Alexander Michael Greiling, Christiane Schwarz, Martin Gierus, Markus Rodehutscord
The objectives of this study were to investigate the digestibility of pumpkin seed cake (PSC) for the rainbow trout, Oncorhynchus mykiss (Walbaum, 1792), and effects on performance and product quality traits of four different fish species when PSC partially replaced fishmeal in extruded diets. A digestibility trial was carried out to determine apparent digestibility coefficients (ADC) for crude protein (CP), ether extract (EE) and gross energy (GE) of PSC fed to rainbow trout. In subsequent growth trials, effects on performance and morphological traits and fillet colour values of four different fish species [rainbow trout; brook trout, Salvelinus fontinalis (Mitchill, 1814); African sharptooth catfish, Clarias gariepinus (Burchell, 1822); and wels catfish, Silurus glanis (Linnaeus, 1758)] were evaluated when 60% of fishmeal protein of a reference diet was replaced by PSC protein (based on digestible CP)...
June 2018: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/29740479/a-new-single-nucleotide-polymorphism-database-for-rainbow-trout-generated-through-whole-genome-resequencing
#10
Guangtu Gao, Torfinn Nome, Devon E Pearse, Thomas Moen, Kerry A Naish, Gary H Thorgaard, Sigbjørn Lien, Yniv Palti
Single-nucleotide polymorphisms (SNPs) are highly abundant markers, which are broadly distributed in animal genomes. For rainbow trout ( Oncorhynchus mykiss ), SNP discovery has been previously done through sequencing of restriction-site associated DNA (RAD) libraries, reduced representation libraries (RRL) and RNA sequencing. Recently we have performed high coverage whole genome resequencing with 61 unrelated samples, representing a wide range of rainbow trout and steelhead populations, with 49 new samples added to 12 aquaculture samples from AquaGen (Norway) that we previously used for SNP discovery...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29737878/involvement-of-cortisol-and-sirtuin1-during-the-response-to-stress-of-hypothalamic-circadian-system-and-food-intake-related-peptides-in-rainbow-trout-oncorhynchus-mykiss
#11
Fatemeh Naderi, Juan Hernández-Pérez, Mauro Chivite, José L Soengas, Jesús M Míguez, Marcos A López-Patiño
Stress is conditioning animal welfare by negatively affecting a wide range of physiological and behavioral functions. This may be applied to circadian physiology and food intake. Cortisol, the stress-related hormone, may mediate such effect of stress, but other indirect mediators might be considered, such as sirtuin1. Then, either the independent modulatory effect or the existence of any interaction between mediators may be responsible. The circadian system is the main modulator of several integrative mechanisms at both central and peripheral levels that are rhythmically presented, thus influencing different processes such as food intake...
May 8, 2018: Chronobiology International
https://www.readbyqxmd.com/read/29733961/dietary-olive-leaf-olea-europea-l-extract-alters-some-immune-gene-expression-levels-and-disease-resistance-to-yersinia-ruckeri-infection-in-rainbow-trout-oncorhynchus-mykiss
#12
Esin Baba, Ümit Acar, Sevdan Yılmaz, Fahriye Zemheri, Sebahattin Ergün
The aim for the present study was to investigate the effects of olive leaf (Olea europea L.) extract (OLE) on the control of Yersinia ruckeri infection in rainbow (Oncorhynchus mykiss) trout and to assess the impact on the expression of immune-related genes in the spleen and serum biochemical parameters of rainbow trout. Five experimental diets were prepared by adding 0.0%, 0.1%, 0.25%, 0.50% and 1.0% of OLE. Each diet was fed to triplicate groups of fish (mean body weight 51.22 ± 3.04 g) twice a day (at 09:00 and 17:00 h) for 60 days...
May 4, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29727836/fish-thermal-habitat-current-use-and-simulation-of-thermal-habitat-availability-in-lakes-of-the-argentine-patagonian-andes-under-climate-change-scenarios-rcp-4-5-and-rcp-8-5
#13
Pablo H Vigliano, Magalí M Rechencq, María V Fernández, Gustavo E Lippolt, Patricio J Macchi
Habitat use in relation to the thermal habitat availability and food source as a forcing factor on habitat selection and use of Percichthys trucha (Creole perch), Oncorhynchus mykiss (rainbow trout), Salmo trutta (brown trout) and Salvelinus fontinalis (brook trout) were determined as well as future potential thermal habitat availability for these species under climate change scenarios Representative Concentration Pathways 4.5 and 8.5. This study was conducted in three interconnected lakes of Northern Patagonia (Moreno Lake system)...
May 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29716943/hepatic-glucose-metabolic-responses-to-digestible-dietary-carbohydrates-in-two-isogenic-lines-of-rainbow-trout
#14
X Song, L Marandel, M Dupont-Nivet, E Quillet, I Geurden, S Panserat
Rainbow trout ( Oncorhynchus mykiss ) was recognized as typical "glucose-intolerant" fish and poor dietary carbohydrate user. Our first objective was to test the effect of dietary carbohydrates itself (without modification of dietary protein intake) on hepatic glucose gene expression (taking into account of the paralogs. The second aim was to research if two isogenic trout lines had different responds to carbohydrate intake, showing one with a better use dietary carbohydrates. Thus, we used two isogenic lines of rainbow trout (named A32h and AB1h) fed with either high carbohydrate diet or low carbohydrate diet for 12 weeks...
May 1, 2018: Biology Open
https://www.readbyqxmd.com/read/29714625/prevalence-and-antimicrobial-resistance-profile-of-listeria-species-isolated-from-farmed-and-on-sale-rainbow-trout-oncorhynchus-mykiss-in-western-iran
#15
Ramin Rezai, Elham Ahmadi, Behnam Salimi
Listeria species are important foodborne pathogens, among which L. monocytogenes and L. ivanovii cause human listeriosis. The purpose of this study was to evaluate the incidence and antimicrobial resistance profiles of Listeria species in farmed and on-sale rainbow trout ( Oncorhynchus mykiss) in Kurdistan province, western Iran. A total of 240 fresh rainbow trout fish (120 samples from farms and 120 samples from retail outlets) were collected and analyzed phenotypically for the presence of Listeria. All Listeria isolates were differentiated with molecular techniques, and L...
May 1, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29710581/interaction-of-carboxylated-cdse-zns-quantum-dots-with-fish-embryos-towards-understanding-of-nanoparticles-toxicity
#16
Ričardas Rotomskis, Živilė Jurgelėnė, Mantas Stankevičius, Milda Stankevičiūtė, Nijolė Kazlauskienė, Kęstutis Jokšas, Danguolė Montvydienė, Vytautas Kulvietis, Vitalijus Karabanovas
Due to colloidal instability even with protective coatings, nanoparticles tend to aggregate in complex environments and possibly interact with biota. In this study, visualization of quantum dots (QDs) interaction with rainbow trout (Oncorhynchus mykiss) embryos was performed. Studies on zebrafish (Danio rerio) and pearl gourami (Trichogaster leerii) embryos have shown that QDs interact with embryos in a general manner and their affects are independent on the type of the embryo. It was demonstrated that carboxylated CdSe/ZnS QDs (4 nM) were aggregating in accumulation media and formed agglomerates on the surface of fish embryos under 1-12 days incubation in deep-well water...
April 23, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29710407/distribution-and-prevalence-of-myxobolus-cerebralis-in-postfire-areas-of-plumas-national-forest-utility-of-environmental-dna-sampling
#17
Christine A Richey, Kirsten V Kenelty, Kristina Van Stone Hopkins, Brittany N Stevens, Beatriz Martínez-López, Samantha M Barnum, Sascha L Hallett, Stephen D Atkinson, Jerri L Bartholomew, Esteban Soto
Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M...
April 30, 2018: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/29710401/infection-by-nanophyetus-salmincola-and-toxic-contaminant-exposure-in-out-migrating-steelhead-from-puget-sound-washington-implications-for-early-marine-survival
#18
M F Chen, S M O'Neill, A J Carey, R H Conrad, B A Stewart, K R Snekvik, G M Ylitalo, P K Hershberger
Out-migrating steelhead Oncorhynchus mykiss from four Puget Sound rivers and associated marine basins of Puget Sound in Washington State were examined for the parasite, Nanophyetus salmincola in 2014 to determine whether recent trends in reduced marine survival are associated with the presence of this pathogen. A subset of steelhead from three of these river-marine basin combinations was analyzed for the presence of persistent organic pollutants (POPs) to assess whether exposure to these contaminants is a contributing factor to their reduced marine survival...
April 30, 2018: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/29709592/the-skin-immune-response-of-rainbow-trout-oncorhynchus-mykiss-walbaum-associated-with-puffy-skin-disease-psd
#19
Lyndsay Christie, Ronny van Aerle, Richard K Paley, David W Verner-Jeffreys, Hannah Tidbury, Matthew Green, Stephen W Feist, Irene Cano
Puffy skin disease (PSD) is an emerging skin condition which affects rainbow trout, Oncorhynchus mykiss (Walbaum). The transmission pattern of PSD suggests an infectious aetiology, however, the actual causative infectious agent(s) remain(s) unknown. In the present study, the rainbow trout epidermal immune response to PSD was characterised. Skin samples from infected fish were analysed and classified as mild, moderate or severe PSD by gross pathology and histological assessment. The level of expression of 26 immune-associated genes including cytokines, immunoglobulins and cell markers were examined by TaqMan qPCR assays...
April 27, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29705987/habitat-availability-and-ontogenetic-shifts-alter-bottlenecks-in-size-structured-fish-populations
#20
Ariane Cantin, John R Post
For species that utilize different habitats throughout their life cycle, the habitat limitation at a given stage can act as a bottleneck on population abundance, impacting density-dependent processes such as individual growth and survival. We explore the influence of habitat limitation on population dynamics by developing a multi-stage population model based on lake-dwelling rainbow trout (Oncorhynchus mykiss) populations where adults occupy the lake habitat but use tributaries for spawning and juvenile rearing...
April 29, 2018: Ecology
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