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https://www.readbyqxmd.com/read/28639694/rancidity-development-of-refrigerated-rainbow-trout-oncorhynchus-mykiss-fillets-comparative-effects-of-in-vivo-and-in-vitro-effects-of-lycopene
#1
Ali Ehsani, Mohammad Sedigh Jasour, Naser Agh, Mohammad Hashemi, Mahdi Khodadadi
BACKGROUND: The problem of lipid oxidation in fish during storage is well known and it is related to both temperature and storage time. Antioxidants could have a main role in limiting deteriorative effects of lipid oxidation in fish. The present study was conducted to investigate the effect of dietary supplement against postmortem addition of lycopene on deterioration of trout fillets during storage at 4±1°C for 12 days. RESULTS: At the end of feeding trial, no significant differences were observed among fatty acid composition of different dietary groups...
June 22, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28636253/estimation-of-vanadium-water-quality-benchmarks-for-the-protection-of-aquatic-life-with-relevance-to-the-athabasca-oil-sands-region-using-species-sensitivity-distributions
#2
Stephanie Schiffer, Karsten Liber
Elevated vanadium (V) concentrations in oil sands coke, which is produced and stored on the site of some major Athabasca Oil Sands companies, could pose a risk to aquatic ecosystems in northern Alberta, Canada, depending on its future storage and utilization. In the present study, V toxicity was determined in reconstituted Athabasca River water to various freshwater organisms, including 2 midge species (Chironomus dilutus and Chironomus riparius; 4-d and 30- to 40-d exposures) and 2 freshwater fish species (Oncorhynchus mykiss and Pimephales promelas; 4-d and 28-d exposures) to facilitate estimation of water quality benchmarks...
June 21, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28634082/cytokine-modulation-by-stress-hormones-and-antagonist-specific-hormonal-inhibition-in-rainbow-trout-oncorhynchus-mykiss-and-gilthead-sea-bream-sparus-aurata-head-kidney-primary-cell-culture
#3
Ali Reza Khansari, David Parra, Felipe E Reyes-López, Lluís Tort
A tight interaction between endocrine and immune systems takes place mainly due to the key role of head kidney in both hormone and cytokine secretion, particularly under stress situations in which the physiological response promotes the synthesis and release of stress hormones which may lead into immunomodulation as side effect. Although such interaction has been previously investigated, this study evaluated for the first time the effect of stress-associated hormones together with their receptor antagonists on the expression of cytokine genes in head kidney primary cell culture (HKPCC) of the freshwater rainbow trout (Oncorhynchus mykiss) and the seawater gilthead sea bream (Sparus aurata)...
June 17, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28628243/using-ultrahigh-resolution-mass-spectrometry-and-toxicity-identification-techniques-to-characterize-the-toxicity-of-oil-sands-process-affected-water-the-case-for-classical-naphthenic-acids
#4
Sarah A Hughes, Ashley Mahaffey, Bryon Shore, Josh Baker, Bruce Kilgour, Christine Brown, Kerry M Peru, John V Headley, Howard C Bailey
Previous assessments of oil sands process-affected water (OSPW) toxicity were hampered by lack of high-resolution analytical analysis, use of non-standard toxicity methods, and variability between OSPW samples. We integrated ultrahigh-resolution mass spectrometry with a Toxicity Identification Evaluation (TIE) approach to quantitatively identify the primary cause of acute toxicity of OSPW to rainbow trout (Oncorhynchus mykiss). The initial characterization of OSPW toxicity indicated that toxicity was associated with non-polar organic compounds, and toxicant(s) were further isolated within a range of discrete methanol fractions that were then subjected to Orbitrap mass spectrometry to evaluate the contribution of naphthenic acid (NA) fraction compounds to toxicity...
June 19, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28602736/effect-of-vitamin-c-on-innate-immune-responses-of-rainbow-trout-oncorhynchus-mykiss-leukocytes
#5
Esther Leal, Carlos Zarza, Carolina Tafalla
Vitamin C, also known as ascorbic acid, is an essential micronutrient that influences a wide variety of physiological processes, including immunological functions. Although the positive effects of vitamin C supplementation on the immunological status of fish has been established in different species, the bases for these positive effects are still unknown. Hence, the aim of our study was to evaluate the in vitro effect of vitamin C on several innate immune functions of rainbow trout (Oncorhynchus mykiss) leukocyte populations...
June 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602677/immune-responses-to-methanolic-extract-of-black-cumin-nigella-sativa-in-rainbow-trout-oncorhynchus-mykiss
#6
Yasemin Celik Altunoglu, Soner Bilen, Ferhat Ulu, Gouranga Biswas
The immune stimulating effects of the methanolic extract of black cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss) was evaluated. Variable concentrations of black cumin methanolic extract [0 (Control), 0.1 and 0.5 g kg(-1) of feed] were individually added to the basal diet and rainbow trout was fed for 30 days to assess the innate immune responses and growth performance. Feed conversion ratio significantly decreased in the group fed with 0.5 g kg(-1) black cumin extract. Respiratory burst activity was observed to be the highest in the 0...
June 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28599186/histological-alterations-in-gills-and-liver-of-rainbow-trout-oncorhynchus-mykiss-after-exposure-to-the-antibiotic-oxytetracycline
#7
S Rodrigues, S C Antunes, B Nunes, A T Correia
The aim of this study was to investigate the histopathological effects of oxytetracycline (OTC) on the gill and liver tissues of rainbow trouts (Oncorhynchus mykiss) following acute (96h: 0.005-50mg/L) and chronic (28days: 0.3125-5μg/L) exposures. Results suggest the existence of a cause-and-effect relationship between the exposure to OTC and tissue damage. Most predominant disorders observed in gills were progressive (e.g. hypertrophy of mucous cells and hyperplasia of epithelial cells) in acute exposure and regressive (e...
May 27, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28596211/low-levels-of-extracellular-glucose-limit-cardiac-anaerobic-metabolism-in-some-species-of-fish
#8
Kathy A Clow, Connie E Short, William R Driedzic
There is a wide interspecific range in plasma glucose levels in teleosts from less than 0.5 to greater than 10 mmol l(-1) Here it is assessed how glucose availability influences glucose metabolism in hearts of Atlantic cod (Gadus morhua), rainbow trout (Oncorhynchus mykiss), lumpfish (Cyclopterus lumpus) and short-horned sculpin (Myoxocephalus scorpius) under normoxic and hypoxic conditions. These species had plasma glucose levels of 5.1, 4.8, 0.9 and 0.5 mmol l(-1), respectively. Rates of glucose metabolism and lactate production were determined in isolated hearts perfused with media containing physiological levels of glucose...
June 8, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28585158/properties-of-probiotics-kocuria-sm1-and-rhodococcus-sm2-isolated-from-fish-guts
#9
S M Sharifuzzaman, Hafizur Rahman, Dawn A Austin, B Austin
This study characterized probiotics Kocuria SM1 and Rhodococcus SM2, which were recovered from the intestinal microbiota of rainbow trout (Oncorhynchus mykiss, Walbaum). The cultures were Gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. Cell multiplication of SM1 and SM2 was observed at 4-37 °C (45 °C for SM1), in 0-20% (w/v) NaCl and at pH 2-11. The viability was not affected when exposed to pepsin at pH 2.0 and 3.0, and pancreatin at pH 8.0. Neither isolates were chrome azurol S-positive for siderophore production...
June 5, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28578488/17%C3%AE-estradiol-increases-non-cpg-methylation-in-exon-1-of-the-rainbow-trout-oncorhynchus-mykiss-myod-gene
#10
Prasanthi P Koganti, Jian Wang, Beth Cleveland, Jianbo Yao
MyoD is an important myogenic transcription factor necessary for the differentiation of myogenic precursor cells (MPC) to form mature myotubes, a process essential for muscle growth. Epigenetic markers such as CpH methylation are known gene regulators that are important for the differentiation process. In this study, we investigated whether DNA methylation is a potential mechanism associated with the ability of 17β-estradiol (E2) to reduce MyoD gene expression and muscle growth in rainbow trout. Rainbow trout received a single intraperitoneal injection of E2 or the injection vehicle (control)...
June 3, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28560280/data-for-itraq-based-quantification-of-the-cardiac-proteome-of-rainbow-trout-oncorhynchus-mykiss-at-rest-and-with-exercise-training
#11
L A Dindia, S L Alderman, T E Gillis
This data article presents the first description of the rainbow trout cardiac ventricle at the level of the proteome, with more than 700 proteins identified and quantified using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS. The abundances of these proteins were compared across 4 durations of moderate exercise training (0, 4, 7, and 14 d), and a total of 107 proteins were differentially abundant during the course of the training program. The differentially abundant proteins are presented here grouped by functional classification...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28551229/biopolymer-coated-nanoliposomes-as-carriers-of-rainbow-trout-skin-derived-antioxidant-peptides
#12
Leila Ramezanzade, Seyed Fakhreddin Hosseini, Maryam Nikkhah
In this study, an antioxidant peptide fraction with a molecular mass<30kDa (PF30) isolated from rainbow trout (Oncorhynchus mykiss) skin gelatin hydrolysates was encapsulated in chitosan-coated nanoliposomes. The mean particle size of liposomal nanovesicles containing PF30 was found to be in the range 163.4-234nm with a low polydispersity index (PDI<0.5); furthermore, the ζ-potential changed from +3.9mV in uncoated liposomes to +45.5mV in biopolymer-coated liposomes. FTIR spectra showed electrostatic interactions as well as hydrogen bonding between phospholipid head groups and amine groups of chitosan...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28548690/rainbow-trout-oncorhynchus-mykiss-immune-response-towards-a-recombinant-vaccine-targeting-the-parasitic-ciliate-ichthyophthirius-multifiliis
#13
L von Gersdorff Jørgensen, P W Kania, K J Rasmussen, A H Mattsson, J Schmidt, A Al-Jubury, A Sander, A Salanti, K Buchmann
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28548689/phenotype-of-aeromonas-salmonicida-sp-salmonicida-cyclic-adenosine-3-5-monophosphate-receptor-protein-crp-mutants-and-its-virulence-in-rainbow-trout-oncorhynchus-mykiss
#14
K Valderrama, M Saravia, J Santander
Precise deletion of genes related to virulence can be used as a strategy to produce attenuated bacterial vaccines. Here, we study the deletion of the cyclic-3',5'-adenosine monophosphate (cAMP) receptor protein (Crp) in Aeromonas salmonicida, the aetiologic agent of furunculosis in marine and freshwater fish. The Crp protein is a conserved global regulator, controlling physiology processes, like sugar utilization. Deletion of the crp gene has been utilized in live attenuated vaccines for mammals, birds and warm water fish...
May 26, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28531838/antimicrobial-agents-triclosan-chloroxylenol-methylisothiazolinone-and-borax-used-in-cleaning-had-genotoxic-and-histopathologic-effects-on-rainbow-trout
#15
Erol Capkin, Tuna Ozcelep, Sevki Kayis, Ilhan Altinok
Triclosan (TRC), chloroxylenol (PCMX) and methylisothiazolinone (MIT) have been commonly used as an antimicrobial in soaps while borax (BRX) is used in household cleaning. After using these chemicals, they are washed down drains and getting into the aquatic ecosystem in which they may affect aquatic living organisms. In the present study, the chronic effects of TRC, PCMX, MIT and BRX on genotoxicity, gene expression and histopathology of rainbow trout (Oncorhynchus mykiss) were evaluated for 40 days under semi static condition...
May 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28530575/effects-of-ergothioneine-rich-mushroom-extracts-on-lipid-oxidation-and-discoloration-in-salmon-muscle-stored-at-low-temperatures
#16
Jade Pahila, Hisayuki Kaneda, Reiko Nagasaka, Tomoyuki Koyama, Toshiaki Ohshima
The effects of supplementing ergothioneine-rich mushroom extracts (ME) on discoloration and lipid oxidation in astaxanthin-pigmented salmon muscles were evaluated. ME (Flammulina velutipes) were added (in vitro) to minced Oncorhynchus mykiss muscles and stored at -10°C. During several weeks of storage, the ME-treated group showed lower lipid hydroperoxide (HPO) accumulation and higher retained astaxanthin levels than in the control group. The effects of adding concentrated ME (Pleurotus cornucopiae) to Oncorhynchus kisutch diets were also tested over a 2-month feeding trial...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530295/dose-and-chemical-species-specific-effects-of-selenium-against-arsenite-toxicity-in-cultured-hepatocytes-of-rainbow-trout-oncorhynchus-mykiss
#17
Ankur Jamwal, Som Niyogi
The present study evaluated the mechanistic underpinnings of the interactive effects of selenium (Se), both inorganic (selenite) and organic (selenomethionine (SeMet)), against arsenite (As-III) cytotoxicity using rainbow trout (Oncorhynchus mykiss) hepatocytes in primary culture. Arsenite is known to induce cytotoxic effects by disrupting cellular redox homeostasis. In contrast, Se is essential for the maintenance of cellular anti-oxidative machinery, but when present above a threshold concentration, can also induce reactive oxygen species (ROS) generation and cause oxidative damage...
June 21, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28525936/heavy-metal-and-arsenic-concentrations-in-rainbow-trout-oncorhynchus-mykiss-farmed-in-a-dam-reservoir-on-the-firat-euphrates-river-risk-based-consumption-advisories
#18
Memet Varol, Gülderen Kurt Kaya, Alper Alp
The concentrations of ten metals in rainbow trout (Oncorhynchus mykiss) farmed in the Karakaya Dam Reservoir (Turkey) on the Firat River were determined. The metal concentrations in rainbow trout did not exceed the maximum permissible levels. Biomagnification factors (BMF) of ten metals were <1, indicating that these metals were not biomagnified. The estimated daily intake (EDI) of each metal was much lower than the respective tolerable daily intake (TDI). The target hazard quotient (THQ) for individual metal and total THQ for combined metals were lower than 1, indicating no health risk for consumers due to the intake of either individual metal or combined metals...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28525709/sensory-and-behavioral-responses-of-a-model-fish-to-oil-sands-process-affected-water-with-and-without-treatment
#19
Megan Reichert, Brian Blunt, Tia Gabruch, Tanja Zerulla, Allison Ralph, Mohamed Gamal El-Din, Bruce R Sutherland, Keith B Tierney
If oil sands process-affected water (OSPW) is to be returned to the environment, a desire is that it not adversely affect aquatic life. We investigated whether a relevant model fish (rainbow trout, Oncorhynchus mykiss) could detect OSPW using its olfactory sense (smell) and whether exposure to it would result in behavioral changes. We also investigated whether ozonation of OSPW, which lowers the concentration of organic compounds attributed with toxicity (naphthenic acids), would ameliorate any observed adverse effects...
June 2, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28525337/molecular-characterization-and-detection-of-enterotoxins-methicillin-resistance-genes-and-antimicrobial-resistance-of-staphylococcus-aureus-from-fish-and-ground-beef
#20
S Arslan, F Özdemir
A total of 120 samples including 40 freshwaterfish(Oncorhynchus mykiss), 40 seawater fish (Sparus aurata) and 40 ground beef samples were examined for the presence of Staphylococcus aureus. The isolates were identified using biochemical tests and a PCR for the species-specific fragment (Sa442) and thermonuclease gene (nucA). The presence of staphylococcal enterotoxin genes (sea, seb, sec, sed and see), toxin genes (eta, etb, tsst), methicillin resistance gene (mecA) and some phenotypic virulence factors was also tested...
March 28, 2017: Polish Journal of Veterinary Sciences
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