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https://www.readbyqxmd.com/read/28213543/functional-characterization-of-a-28-kb-catabolic-island-from-pseudomonas-sp-m1-involved-in-biotransformation-of-%C3%AE-myrcene-and-related-plant-derived-volatiles
#1
Pedro Soares-Castro, Pedro Montenegro-Silva, Hermann J Heipieper, Pedro M Santos
Pseudomonas sp. M1 is able to mineralize highly hydrophobic and recalcitrant compounds such as benzene, phenol and their methylated/halogenated derivatives, as well as the backbone of several monoterpenes. The ability to use such spectrum of compounds as sole carbon source is, most probably, associated with a genetic background evolved under different environmental constraints. The outstanding performance of M1 strain regarding β-myrcene catabolism was elucidated in this work, with focus on the biocatalytical potential of β-myrcene-associated core-code, comprised in a 28-kb genomic island (GI), predicted to be organized in 8 transcriptional units...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28211326/evaluation-of-in%C3%A2-vivo-and-in%C3%A2-vitro-toxicological-and-genotoxic-potential-of-aluminum-chloride
#2
Letícia Nazareth Fernandes Paz, Laís Mesquita Moura, Danielle Cristinne A Feio, Mirella de Souza Gonçalves Cardoso, Wagner Luiz O Ximenes, Raquel C Montenegro, Ana Paula N Alves, Rommel R Burbano, Patrícia Danielle L Lima
Aluminum and its compounds are common contaminants of water and food, as well as medications and cosmetics. The wide distribution of the element facilitates the demand for detailed studies of its biological and toxicological effects. This work aimed to evaluate the possible genotoxic and toxic activity resulting from in vivo and in vitro exposure to Al. For in vivo analysis, 40 Swiss mice were used, various concentrations of hydrated aluminum chloride were administered orally. They were analyzed for possible genic activity and metal cytotoxicity using a micronucleus test (MN), and for toxicity through histopathological evaluation of the extracted organs...
February 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28210960/first-description-of-the-male-and-redescription-of-the-female-of-ixodes-tapirus-kohls-1956-acari-ixodidae-a-parasite-of-tapirs-perissodactyla-tapiridae-from-the-mountains-of-colombia-costa-rica-and-panama
#3
Dmitry A Apanaskevich, Lillian G Domínguez, Sugeys S Torres, Juan A Bernal, Victor M Montenegro, Sergio E Bermúdez
The male of Ixodes tapirus Kohls, 1956 (Acari: Ixodidae) is described for the first time and the female is redescribed in greater detail. Adults of I. tapirus are similar to those of Ixodes guatemalensis Kohls, 1956, Ixodes lasallei Méndez & Ortiz, 1958, Ixodes montoyanus Cooley, 1944 and Ixodes venezuelensis Kohls, 1953 but can be distinguished by their overall size, the amount of sclerotisation of the conscutum and accessory plates, the shape of the scutum, the number of punctations and their pattern on the conscutum and scutum, the depth of the punctations on the basis capituli dorsally, the shape and size of the porose areas and the size and shape of the auriculae...
February 15, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28203467/costello-syndrome-and-umbilical-ligament-rhabdomyosarcoma-in-two-pediatric-patients-case-reports-and-review-of-the-literature
#4
Carlos Sánchez-Montenegro, Alejandra Vilanova-Sánchez, Saturnino Barrena-Delfa, Jair Tenorio, Fernando Santos-Simarro, Sixto García-Miñaur, Pablo Lapunzina, Leopoldo Martínez-Martínez
Costello syndrome is caused by heterozygous de novo missense mutations in the protooncogene HRAS with tumor predisposition, especially rhabdomyosarcoma. We here report two pediatric patients with Costello syndrome and umbilical ligament rhabdomyosarcoma. A review of the literature published in English in MEDLINE from January 1971 to June 2016 using the search terms "Costello syndrome" and "rhabdomyosarcoma" was performed, including two new cases that we describe. Twenty-six patients with Costello syndrome and rhabdomyosarcoma were recorded with mean age of diagnosis of 2 years and 8 months...
2017: Case Reports in Genetics
https://www.readbyqxmd.com/read/28202286/equatorial-wrinkles-in-the-human-lens-capsule
#5
H J Burd, G A Montenegro, L P Cortés, R I Barraquer, R Michael
Equatorial wrinkles, or crenations, have been previously observed around the equator in coronal images of the human ocular lens. However, wrinkles are typically not apparent when the lens is viewed from saggital directions. In the current paper, the existence and geometry of these wrinkles is shown to be consistent with a mechanical model of the isolated lens, in which the capsule is held in a state of residual tension by a spatially uniform internal pressure. The occurrence of equatorial wrinkles is therefore seen to be a mechanical consequence of the spheroidal shape of the lens capsule and an excess intralenticular pressure...
February 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28194704/challenges-and-future-prospects-for-the-delivery-of-biologics-oral-mucosal-pulmonary-and-transdermal-routes
#6
Javier O Morales, Kristin R Fathe, Ashlee Brunaugh, Silvia Ferrati, Song Li, Miguel Montenegro-Nicolini, Zeynab Mousavikhamene, Jason T McConville, Mark R Prausnitz, Hugh D C Smyth
Biologic products are large molecules such as proteins, peptides, nucleic acids, etc., which have already produced many new drugs for clinical use in the last decades. Due to the inherent challenges faced by biologics after oral administration (e.g., acidic stomach pH, digestive enzymes, and limited permeation through the gastrointestinal tract), several alternative routes of administration have been investigated to enable sufficient drug absorption into systemic circulation. This review describes the buccal, sublingual, pulmonary, and transdermal routes of administration for biologics with relevant details of the respective barriers...
February 13, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28194580/hepatitis-c-virus-genotype-3a-in-a-population-of-injecting-drug-users-in-montenegro-bayesian-and-evolutionary-analysis
#7
Boban Mugosa, Eleonora Cella, Alessia Lai, Alessandra Lo Presti, Aletheia Blasi, Zoran Vratnica, Danijela Vujoševic, Erika Ebranati, Dragan Lauševic, Michele Guarino, Gianguglielmo Zehender, Teresa Milano, Stefano Pascarella, Silvia Spoto, Silvia Angeletti, Massimo Ciccozzi
Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this population. Sixty-four HCV-positive IDUs in Montenegro were enrolled between 2013 and 2014, and the NS5B gene was sequenced. The Bayesian analysis showed that the most prevalent subtype was HCV-3a. Phylogenetic data showed that HCV-3a reached Montenegro in the late 1990s, causing an epidemic that exponentially grew between the 1995 and 2005...
February 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28187680/revision-of-the-flightless-click-beetle-genus-i-dima-i-charpentier-1825-coleoptera-elateridae-dimini-in-the-balkan-peninsula
#8
Josef Mertlik, Tamás Németh, Robin Kundrata
The genus Dima Charpentier, 1825 in the Balkan Peninsula and surrounding areas was revised based on the adult semaphoronts. Altogether 33 species are known from the investigated area, of which nine are new for science: D. bruhai sp. nov. (Greece), D. dusaneki sp. nov. (Macedonia), D. fialai sp. nov. (Macedonia), D. kozufensis sp. nov. (Greece, Macedonia), D. orientalis sp. nov. (Bulgaria), D. pelikani sp. nov. (Albania, Montenegro), D. pelionensis sp. nov. (Greece), D. vonickai sp. nov. (Albania), and D. zbuzeki sp...
January 13, 2017: Zootaxa
https://www.readbyqxmd.com/read/28167885/characterization-of-pseudomonas-syringae-pv-syringae-causal-agent-of-citrus-blast-of-mandarin-in-montenegro
#9
Žarko Ivanović, Tatjana Perović, Tatjana Popović, Jovana Blagojević, Nenad Trkulja, Snježana Hrnčić
Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P...
February 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28166266/medication-related-factors-associated-with-health-related-quality-of-life-in-patients-older-than-65-years-with-polypharmacy
#10
Alonso Montiel-Luque, Antonio Jesús Núñez-Montenegro, Esther Martín-Aurioles, Jose Carlos Canca-Sánchez, Maria Carmen Toro-Toro, José Antonio González-Correa
METHODS AND DESIGN: Objective: To describe the relationship between medication-related factors and the health-related quality of life in patients older than 65 years who use multiple medications (polypharmacy). Design: Cross-sectional descriptive study. Setting: Primary care. Participants: Patients older than 65 years who use multiple medications (n = 375). Measurements: The main outcome measure was health-related quality of life according to the EuroQol-5D instrument. Sociodemographic, clinical and medication-related variables were recorded during home interviews...
2017: PloS One
https://www.readbyqxmd.com/read/28151764/increased-resistance-to-flow-and-ventilator-failure-secondary-to-faulty-co2-absorbent-insert-not-detected-during-automated-anesthesia-machine-check-a-case-report
#11
Ingrid Moreno-Duarte, Julio Montenegro, Konstantin Balonov, Roman Schumann
Most modern anesthesia workstations provide automated checkout, which indicates the readiness of the anesthesia machine. In this case report, an anesthesia machine passed the automated machine checkout. Minutes after the induction of general anesthesia, we observed a mismatch between the selected and delivered tidal volumes in the volume auto flow mode with increased inspiratory resistance during manual ventilation. Endotracheal tube kinking, circuit obstruction, leaks, and patient-related factors were ruled out...
February 1, 2017: A & A Case Reports
https://www.readbyqxmd.com/read/28148778/lipid-rafts-of-mouse-liver-contain-nonextended-and-extended-acetylcholinesterase-variants-along-with-m3-muscarinic-receptors
#12
María Fernanda Montenegro, Juan Cabezas-Herrera, F Javier Campoy, Encarnación Muñoz-Delgado, Cecilio J Vidal
The observation of acetylcholinesterase (AChE) type H (AChEH), which is the predominant AChE variant in visceral organs and immune cells, in lipid rafts of muscle supports functional reasons for the raft targeting of glypiated AChEH The search for these reasons revealed that liver AChE activity is mostly confined to rafts and that the liver is able to make N-extended AChE variants and target them to rafts. These results prompted us to test whether AChE and muscarinic receptors existed in the same raft. Isolation of flotillin-2-rich raft fractions by their buoyancy in sucrose gradients, followed by immunoadsorption and matrix-assisted laser desorption ionization-time of flight-mass spectrometry application, gave the following results: 1) most hepatic AChE activity emanates from AChE-H mRNA, and its product, glypiated AChEH, accumulates in rafts; 2) N-extended N-AChE readthrough variant, nonglypiated N-AChEH, and N-AChE tailed variant were all identified in liver rafts; and 3) M3 AChRs were observed in rafts, and coprecipitation of raft-confined N-AChE and M3 receptors by using anti-M3 antibodies showed that enzyme and receptor reside in the same raft unit...
February 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28137425/cognitive-evaluation-for-the-diagnosis-of-alzheimer-s-disease-based-on-turing-test-and-virtual-environments
#13
Juan Manuel Fernandez Montenegro, Vasileios Argyriou
Alzheimer's screening tests are commonly used by doctors to diagnose the patient's condition and stage as early as possible. Most of these tests are based on pen-paper interaction and do not embrace the advantages provided by new technologies. This paper proposes novel Alzheimer's screening tests based on virtual environments and game principles using new immersive technologies combined with advanced Human Computer Interaction (HCI) systems. These new tests are focused on the immersion of the patient in a virtual room, in order to mislead and deceive the patient's mind...
January 27, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28121183/betalain-rich-concentrate-supplementation-improves-exercise-performance-and-recovery-in-competitive-triathletes
#14
RANDOMIZED CONTROLLED TRIAL
Cristhian F Montenegro, David A Kwong, Zev A Minow, Brian A Davis, Christina F Lozada, Gretchen A Casazza
We aimed to determine the effects of a betalain-rich concentrate (BRC) of beetroots, containing no sugars or nitrates, on exercise performance and recovery. Twenty-two (9 men and 13 women) triathletes (age, 38 ± 11 years) completed 2 double-blind, crossover, randomized trials (BRC and placebo) starting 7 days apart. Each trial was preceded by 6 days of supplementation with 100 mg·day(-1) of BRC or placebo. On the 7th day of supplementation, exercise trials commenced 120 min after ingestion of 50 mg BRC or placebo and consisted of 40 min of cycling (75 ± 5% maximal oxygen consumption) followed by a 10-km running time trial (TT)...
February 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28104258/the-impact-of-betaplus-program-on-patient-treatment-satisfaction-with-interferon-beta-1b-in-multiple-sclerosis-multicentric-cross-sectional-survey-in-the-western-balkan-countries
#15
Jelena Drulovic, Mirjana Cukic, Sanja Grgic, Evica Dincic, Ranko Raicevic, Congor Nadj, Gordana Toncev, Slobodan Vojinovic, Sarlota Mesaros, Darija Kisic Tepavcevic, Irena Dujmovic, Daliborka Tadic, Svetlana Miletic-Drakulic, Jelena Dackovic, Smiljana Kostic, Jevto Erakovic, Lorand Sakalas, Dejan Savic, Vesna Suknjaja, Vanja Martinovic, Gorica Maric, Tatjana Pekmezovic
BACKGROUND: Long-term treatment adherence to disease-modifying drugs (DMDs) may have significant impact on clinical outcomes in multiple sclerosis (MS). It has been recently emphasized that low treatment satisfaction (TS) may be an important factor for achieving high rates of treatment adherence. Interferon (IFN) beta-1b was the first DMD approved for the treatment of MS. The aims of our study were to assess TS in subjects with relapsing-remitting (RR) MS treated with IFN beta-1b in Serbia, Montenegro and the Republika Srpska, Bosnia and Herzegovina (B&H), and additionally, to evaluate the impact of patient support program on TS and adherence...
January 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/28093040/cadmium-in-liver-and-kidneys-of-domestic-balkan-and-alpine-dairy-goat-breeds-from-montenegro-and-serbia
#16
Vladimir Tomović, Marija Jokanović, Mila Tomović, Milana Lazović, Branislav Šojić, Snežana Škaljac, Maja Ivić, Sunčica Kocić-Tanackov, Igor Tomašević, Aleksandra Martinović
Concentrations of cadmium (Cd) were determined in the samples of 144 animals around 1 and of 144 animals around 4 years old. Cd was analyzed by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES), after microwave digestion. Cd concentrations were higher (p < 0.05) in kidney than in liver and higher (p < 0.05) in older animals than in young ones. In domestic Balkan goat which was raised in a free-ranged system Cd accumulation was lower (p < 0.05) than in Alpine goat raised in an intensive production system...
January 16, 2017: Food Additives & Contaminants. Part B, Surveillance
https://www.readbyqxmd.com/read/28079240/new-index-for-the-diagnosis-of-liver-fibrosis-in-schistosomiasis-mansoni
#17
Ana Virgínia Matos Sá Barreto, Vinícius Martins Alecrim, Tibério Batista de Medeiros, Ana Lúcia Coutinho Domingues, Edmundo Pessoa Lopes, João Roberto Maciel Martins, Helena Bonciani Nader, George Tadeu Nunes Diniz, Silvia Maria Lucena Montenegro, Clarice Neuenschwander Lins de Morais
BACKGROUND: - Periportal fibrosis is the major pathological consequence of the Schistosoma mansoni infection. OBJECTIVE: - To evaluate the accuracy of serum markers and to construct an index to assess fibrosis. METHODS: - Patients (n=116) with schistosomiasis were evaluated by ultrasound scan and measurements of serum levels of aminotransferases, γ-glutamyl transferase, alkaline phosphatase, hyaluronic acid, cytokines and platelets. Ultrasound images were used to evaluate the fibrosis using Niamey's classification and identified 19 patients without periportal fibrosis (patterns A and B), 48 with mild to moderate fibrosis (C and D) and 49 with advanced fibrosis (E and F)...
January 2017: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/28074986/-recommendations-for-the-management-of-pancreatic-cancer-type-adenocarcinoma-a-consensus-statement-reached-during-the-2015-latin-american-symposium-on-gastroenterological-oncology
#18
Christian Caglevic, Jorge Gallardo, Marcela de la Torre, Mauricio Mahave, Bettina Müller, Sebastián Solé, Yuri Moscoso, Hernán De La Fuente, Juan Carlos Roa, Sebastián Hoefler, Jean M Butte, Pablo González M, Juan Manuel O'Connor, Javiera Torres, Verónica Pérez Encalada, Daniel Alarcón Cano, Luis Ubillos, Christian Rolfo, Alejo Lingua, Consuelo Díaz Romero, Alejandro Padilla Rosciano, Viviana Cuartero, Germán Calderillo Ruiz, Gilberto Schwartsmann, Xavier Kon Jara, Andrés Andrade G, Luis Mas López, Olga Barajas, Marcela Carballido, Hanns Lembach, Lena Morillas G, Enrique Roca, José Lobatón, Paola Montenegro B, Andrés Yepes, Hugo Marsiglia
Pancreatic cancer is a malignancy of great impact in developed countries and is having an increasing impact in Latin America. Incidence and mortality rates are similar for this cancer. This is an important reason to offer to the patients the best treatments available. During the Latin American Symposium of Gastroenterology Oncology (SLAGO) held in Viña del Mar, Chile, in April 2015, a multidisciplinary group of specialists in the field met to discuss about this disease. The main conclusions of this meeting, where practitioners from most of Latin American countries participated, are listed in this consensus that seek to serve as a guide for better decision making for patients with pancreatic cancer in Latin America...
October 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/28069315/long-term-non-isothermal-reactive-transport-model-of-compacted-bentonite-concrete-and-corrosion-products-in-a-hlw-repository-in-clay
#19
Alba Mon, Javier Samper, Luis Montenegro, Acacia Naves, Jesús Fernández
Radioactive waste disposal in deep geological repositories envisages engineered barriers such as carbon-steel canisters, compacted bentonite and concrete liners. The stability and performance of the bentonite barrier could be affected by the corrosion products at the canister-bentonite interface and the hyper-alkaline conditions caused by the degradation of concrete at the bentonite-concrete interface. Additionally, the host clay formation could also be affected by the hyper-alkaline plume at the concrete-clay interface...
December 24, 2016: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/28050539/comparative-analysis-of-pain-in-patients-who-underwent-total-knee-replacement-regarding-the-tourniquet-pressure
#20
Marcos George de Souza Leão, Gladys Pedrosa Martins Neta, Lucas Inoue Coutinho, Thiago Montenegro da Silva, Yacov Machado Costa Ferreira, Waryla Raissa Vasconcelos Dias
OBJECTIVES: To evaluate through the visual analog scale (VAS) the pain in patients undergoing total knee replacement (TKR) with different pressures of the pneumatic tourniquet. METHODS: An observational, randomized, descriptive study on an analytical basis, with 60 patients who underwent TKR, divided into two groups, which were matched: a group where TKR was performed with tourniquet pressures of 350 mmHg (standard) and the other with systolic blood pressure plus 100 mmHg (P + 100)...
November 2016: Revista Brasileira de Ortopedia
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