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https://www.readbyqxmd.com/read/28735393/generation-of-a-stress-inducible-luminescent-arabidopsis-and-its-use-in-genetic-screening-for-stress-responsive-gene-deregulation-mutants
#1
Si-In Yu, Byeong-Ha Lee
In order to understand plant stress tolerance and its application, it is important to identify the signaling components involved in the stress-regulated gene expression. One initial step for this is generation of a stress-inducible luminescent Arabidopsis and its use in genetic mutant screening. Here, we describe how to generate a transgenic Arabidopsis line harboring a single copy of the STABILIZED1 (STA1) promoter-driven luciferase transgene (STA1p-LUC) as an example. STA1 is a pre-mRNA splicing factor Prp6p homolog and is induced by cold and heat stresses...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28731217/small-rnas-in-regulating-temperature-stress-response-in-plants
#2
Qing Liu, Shijuan Yan, Tifeng Yang, Shaohong Zhang, Yue-Qin Chen, Bin Liu
Due to global climate change, temperature stress has become one of the primary causes of crop losses worldwide. Much progress has been made in unraveling the complex stress response mechanisms in plants, particularly in the identification of temperature stress responsive protein-coding genes. Recently discovered microRNAs (miRNAs) and endogenous small-interfering RNAs (siRNAs) have also been demonstrated as important players in plant temperature stress response. Using high-throughput sequencing, many small RNAs, especially miRNAs, have been identified to be triggered by cold or heat...
July 21, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/28727798/cloning-and-expression-of-genes-encoding-heat-shock-proteins-in-liriomyza-trifolii-and-comparison-with-two-congener-leafminer-species
#3
Ya-Wen Chang, Jing-Yun Chen, Ming-Xing Lu, Yuan Gao, Zi-Hua Tian, Wei-Rong Gong, Chang-Sheng Dong, Yu-Zhou Du
The polyphagous agromyzid fly, Liriomyza trifolii, is a significant and important insect pest of ornamental and vegetable crops worldwide. The adaptation of insects to different environments is facilitated by heat shock proteins (HSPs), which play an important role in acclimation to thermal stress. In this study, we cloned and characterized five HSP-encoding genes of L. trifolii (Lthsp20, Lthsp40, Lthsp60, Lthsp70, and Lthsp90) and monitored their expression under different thermal stresses using real-time quantitative PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28724078/genome-wide-association-study-of-developing-leaves-heat-tolerance-during-vegetative-growth-stages-in-a-sorghum-association-panel
#4
Junping Chen, Ratan Chopra, Chad Hayes, Geoffrey Morris, Sandeep Marla, John Burke, Zhanguo Xin, Gloria Burow
Heat stress reduces grain yield and quality worldwide. Enhancing heat tolerance of crops at all developmental stages is one of the essential strategies required for sustaining agricultural production especially as frequency of temperature extremes escalates in response to climate change. Although heat tolerance mechanisms have been studied extensively in model plant species, little is known about the genetic control underlying heat stress responses of crop plants at the vegetative stage under field conditions...
July 2017: Plant Genome
https://www.readbyqxmd.com/read/28720381/tolerance-to-baked-and-fermented-cow-s-milk-in-children-with-ige-mediated-and-non-ige-mediated-cow-s-milk-allergy-in-patients-under-two-years-of-age
#5
A Uncuoglu, N Yologlu, I E Simsek, Z S Uyan, M Aydogan
BACKGROUND: IgE-mediated cow's milk allergy (CMA) has been shown consistent in milder heated-milk tolerant and severe heated-milk reactant groups in patients older than two years. Little is known whether fermentation of milk gives rise to similar clinical phenotypes. We aimed to determine the influence of extensively heated and fermented cow's milk on the IgE-mediated and non-IgE-mediated CMA in children younger than two years. METHODS: Subjects followed with the diagnosis of IgE-mediated and non-IgE-mediated CMA for at least six months underwent unheated milk challenge...
July 15, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28718632/systematic-approaches-to-efficiently-produce-2-3-butanediol-in-a-marine-cyanobacterium
#6
Nicole E Nozzi, Anna E Case, Austin L Carroll, Shota Atsumi
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues remain in scale-up. Use of the cyanobacterium Synechococcus sp. PCC 7002 (7002) as a chemical production chassis could address a number of these issues given the higher tolerance to salt, light, and heat as well as the fast growth rate of 7002 in comparison to traditional model cyanobacteria such as Synechococcus elongatus PCC 7942 and Synechocystis sp...
July 18, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28714602/genome-wide%C3%A2-identification-and-characterization%C3%A2-of%C3%A2-hsp-gene-superfamily-in-whitefly-bemisia-tabaci-and-expression-profiling%C3%A2-analysis%C3%A2-under-temperature-stress
#7
Xin-Ru Wang, Chao Wang, Fei-Xue Ban, Dan-Tong Zhu, Shu-Sheng Liu, Xiao-Wei Wang
Heat shock proteins (Hsp) are essential molecular chaperones that play important roles in the stress stimulation of insects. Bemisia tabaci, a phloem feeder and invasive species, can cause extensive crop damage through direct feeding and transmission of plant viruses. Here we employed comprehensive genomics approaches to identity HSP superfamily members in Middle East Asia Minor 1 whitefly genome. In total, we identified 26 Hsp genes, including 3 Hsp90, 17 Hsp70, 1 Hsp60 and 5 sHSP (small heat shock protein, sHSP) genes...
July 17, 2017: Insect Science
https://www.readbyqxmd.com/read/28711268/short-communication-implementation-of-a-breeding-value-for-heat-tolerance-in-australian-dairy-cattle
#8
Thuy T T Nguyen, Phil J Bowman, Mekonnen Haile-Mariam, Gert J Nieuwhof, Benjamin J Hayes, Jennie E Pryce
Excessive ambient temperature and humidity can impair milk production and fertility of dairy cows. Selection for heat-tolerant animals is one possible option to mitigate the effects of heat stress. To enable selection for this trait, we describe the development of a heat tolerance breeding value for Australian dairy cattle. We estimated the direct genomic values of decline in milk, fat, and protein yield per unit increase of temperature-humidity index (THI) using 46,726 single nucleotide polymorphisms and a reference population of 2,236 sires and 11,853 cows for Holsteins and 506 sires and 4,268 cows for Jerseys...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28709955/neonatal-injury-results-in-sex-dependent-nociceptive-hypersensitivity-and-social-behavioral-deficits-during-adolescence-without-altering-morphine-response
#9
Nikita N Burke, Tuan Trang
Neonatal injury is associated with persistent changes in sensory function and altered nociceptive thresholds that give rise to aberrant pain sensitivity in later life. While these changes are well documented in adult rodents, little is known about the consequences of neonatal injury during adolescence. Since adolescence is a critical developmental period during which persistent pain conditions can arise, we examined the impact of neonatal injury on nociception, social behavior and response to morphine in adolescent Sprague Dawley rats...
July 11, 2017: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28708887/carried-over-heat-stress-in-the-egg-stage-reduces-subsequent-performance-in-a-butterfly
#10
Michael Klockmann, Friederike Kleinschmidt, Klaus Fischer
Increasing heat stress caused by anthropogenic climate change may pose a substantial challenge to biodiversity due to associated detrimental effects on survival and reproduction. Therefore, heat tolerance has recently received substantial attention, but its variation throughout ontogeny and effects carried over from one developmental stage to another remained largely neglected. To explore to what extent stress experienced early in life affects later life stages, we here investigate effects of heat stress experienced in the egg stage throughout ontogeny in the tropical butterfly Bicyclus anynana...
2017: PloS One
https://www.readbyqxmd.com/read/28708766/is-the-rationale-more-important-than-deception-a-randomized-controlled-trial-of-openlabel-placebo-analgesia
#11
Cosima Locher, Antje Frey Nascimento, Irving Kirsch, Joe Kossowsky, Andrea Meyer, Jens Gaab
Research on open-label placebos questions whether deception is a necessary characteristic of placebo effects. Yet, comparisons between open-label and deceptive placebos are lacking. We therefore assessed effects of open-label and deceptive placebos in comparison to no treatment with a standardized experimental heat pain paradigm in a RCT in healthy participants. Participants (N = 160) were randomly assigned to no treatment (NT), open-label placebo without rationale (OPR-), open-label placebo with rationale (OPR+) and deceptive placebo (DP)...
July 12, 2017: Pain
https://www.readbyqxmd.com/read/28707371/individuals-with-sickle-cell-disease-have-a-significantly-greater-vasoconstriction-response-to-thermal-pain-than-controls-and-have-significant-vasoconstriction-in-response-to-anticipation-of-pain
#12
Maha Khaleel, Mammen Puliyel, Payal Shah, John Sunwoo, Roberta M Kato, Patjanaporn Chalacheva, Wanwara Thuptimdang, Jon Detterich, John C Wood, Jennie Tsao, Lonnie Zeltzer, Richard Sposto, Michael C K Khoo, Thomas D Coates
The painful vaso-occlusive crises (VOC) that characterize sickle cell disease (SCD) progress over hours from the asymptomatic steady-state. SCD patients report that VOC can be triggered by stress, cold exposure and pain itself. We anticipated that pain could cause neural-mediated vasoconstriction, decreasing regional blood flow and promoting entrapment of sickle cells in the microvasculature. Therefore, we measured microvascular blood flow in the fingers of both hands using plethysmography and laser-Doppler flowmetry while applying a series of painful thermal stimuli on the right forearm in 23 SCD patients and 25 controls...
July 13, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28706528/molecular-mechanisms-behind-the-physiological-resistance-to-intense-transient-warming-in-an-iconic-marine-plant
#13
Lazaro Marín-Guirao, Laura Entrambasaguas, Emanuela Dattolo, Juan M Ruiz, Gabriele Procaccini
The endemic Mediterranean seagrass Posidonia oceanica is highly threatened by the increased frequency and intensity of heatwaves. Meadows of the species offer a unique opportunity to unravel mechanisms marine plants activate to cope transient warming, since their wide depth distribution impose divergent heat-tolerance. Understanding these mechanisms is imperative for their conservation. Shallow and deep genotypes within the same population were exposed to a simulated heatwave in mesocosms, to analyze their transcriptomic and photo-physiological responses during and after the exposure...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28702937/genetic-engineering-strategies-for-biotic-and-abiotic-stress-tolerance-and-quality-enhancement-in-horticultural-crops-a-comprehensive-review
#14
REVIEW
Nehanjali Parmar, Kunwar Harendra Singh, Deepika Sharma, Lal Singh, Pankaj Kumar, J Nanjundan, Yasin Jeshima Khan, Devendra Kumar Chauhan, Ajay Kumar Thakur
Genetic engineering technique offers myriads of applications in improvement of horticultural crops for biotic and abiotic stress tolerance, and produce quality enhancement. During last two decades, a large number of transgenic horticultural crops has been developed and more are underway. A number of genes including natural and synthetic Cry genes, protease inhibitors, trypsin inhibitors and cystatin genes have been used to incorporate insect and nematode resistance. For providing protection against fungal and bacterial diseases, various genes like chitinase, glucanase, osmotin, defensin and pathogenesis-related genes are being transferred to many horticultural crops world over...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28702041/characterization-of-bip-genes-from-pepper-capsicum-annuum-l-and-the-role-of-cabip1-in-response-to-endoplasmic-reticulum-and-multiple-abiotic-stresses
#15
Hu Wang, Huanhuan Niu, Yufei Zhai, Minghui Lu
Adverse environmental conditions have a detrimental impact on crop growth and development, and cause protein denaturation or misfolding. The binding protein (BiP) plays an important protective role by alleviating endoplasmic reticulum (ER) stress induced by misfolded proteins. In this study, we characterized three BiP genes (CaBiP1, CaBiP2, and CaBiP3) in pepper, an economically important vegetable and spice species. The role of CaBiP1 in plant tolerance to ER stress and adverse environmental conditions (including heat, salinity, osmotic and drought stress) were investigated...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28695381/development-of-a-method-to-assess-the-ichthyotoxicity-of-the-harmful-marine-microalgae-karenia-spp-using-gill-cell-cultures-from-red-sea-bream-pagrus-major
#16
Nobuyuki Ohkubo, Yuji Tomaru, Haruo Yamaguchi, Saho Kitatsuji, Kazuhiko Mochida
The present study reports the development of a method to investigate ichthyotoxicity of harmful marine microalgae using cultured red sea bream (Pagrus major) gill cells. The cultured gill cells formed adherent 1-2 layers on the bottom of the culture plate and could tolerate seawater exposure for 4 h without significant alteration in cell survival. The microalgae Karenia mikimotoi, Karenia papilionacea, K. papilionacea phylotype-I, and Heterosigma akashiwo were cultured, then directly exposed to gill cells...
July 11, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28694818/insights-into-the-molecular-events-that-regulate-heat-induced-chilling-tolerance-in-citrus-fruits
#17
María T Lafuente, Beatriz Establés-Ortíz, Luis González-Candelas
Low non-freezing temperature may cause chilling injury (CI), which is responsible for external quality deterioration in many chilling-sensitive horticultural crops. Exposure of chilling-sensitive citrus cultivars to non-lethal high-temperature conditioning may increase their chilling tolerance. Very little information is available about the molecular events involved in such tolerance. In this work, the molecular events associated with the low temperature tolerance induced by heating Fortune mandarin, which is very sensitive to chilling, for 3 days at 37°C prior to cold storage is presented...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28694815/co-overexpression-of-the-constitutively-active-form-of-osbzip46-and-aba-activated-protein-kinase-sapk6-improves-drought-and-temperature-stress-resistance-in-rice
#18
Yu Chang, Ba Hoanh Nguyen, Yongjun Xie, Benze Xiao, Ning Tang, Wenliu Zhu, Tongmin Mou, Lizhong Xiong
Drought is one of the major abiotic stresses threatening rice (Oryza sativa) production worldwide. Drought resistance is controlled by multiple genes, and therefore, a multi-gene genetic engineering strategy is theoretically useful for improving drought resistance. However, the experimental evidence for such a strategy is still lacking. In this study, a few drought-responsive genes from rice were assembled by a multiple-round site-specific assembly system, and the constructs were introduced into the rice cultivar KY131 via Agrobacterium-mediated transformation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28693422/relationships-between-drought-heat-and-air-humidity-responses-revealed-by-transcriptome-metabolome-co-analysis
#19
Elisabeth Georgii, Ming Jin, Jin Zhao, Basem Kanawati, Philippe Schmitt-Kopplin, Andreas Albert, J Barbro Winkler, Anton R Schäffner
BACKGROUND: Elevated temperature and reduced water availability are frequently linked abiotic stresses that may provoke distinct as well as interacting molecular responses. Based on non-targeted metabolomic and transcriptomic measurements from Arabidopsis rosettes, this study aims at a systematic elucidation of relevant components in different drought and heat scenarios as well as relationships between molecular players of stress response. RESULTS: In combined drought-heat stress, the majority of single stress responses are maintained...
July 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28689860/the-expression-pattern-of-hsp70-plays-a-critical-role-in-thermal-tolerance-of-marine-demersal-fish-multilevel-responses-of-paralichthys-olivaceus-and-its-hybrids-p-%C3%A2-olivaceus-%C3%A2-%C3%A2-%C3%A3-p-%C3%A2-dentatus-%C3%A2-to-chronic-and-acute-heat-stress
#20
Yifan Liu, Daoyuan Ma, Chunyan Zhao, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Yanfeng Wang, Qinghua Liu, Jun Li
Ocean warming has multifaceted impacts on marine organisms. This study investigated the different responses of Paralichthys olivaceus and the hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress. By comparing their survival, behavioural and histological changes, we found that the hybrids possess a better thermal tolerance with a higher cumulative survival rate (CSR), relatively fewer behavioural changes and less gill damage. Moreover, we analysed the relationship between thermal tolerance and the hsp70 expression pattern and found that thermal tolerant species (the hybrids) exhibited higher threshold induction temperature, shorter durations, stronger magnitudes and a delay in hsp70 expression...
June 23, 2017: Marine Environmental Research
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