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https://www.readbyqxmd.com/read/28225818/heat-induced-radiolabeling-and-click-chemistry-a-powerful-combination-for-generating-multifunctional-nanomaterials
#1
Hushan Yuan, Moses Q Wilks, Georges El Fakhri, Marc D Normandin, Charalambos Kaittanis, Lee Josephson
A key advantage of nanomaterials for biomedical applications is their ability to feature multiple small reporter groups (multimodality), or combinations of reporter groups and therapeutic agents (multifunctionality), while being targeted to cell surface receptors. Here a facile combination of techniques for the syntheses of multimodal, targeted nanoparticles (NPs) is presented, whereby heat-induced-radiolabeling (HIR) labels NPs with radiometals and so-called click chemistry is used to attach bioactive groups to the NP surface...
2017: PloS One
https://www.readbyqxmd.com/read/28223714/comparative-physiological-and-transcriptomic-analyses-provide-integrated-insight-into-osmotic-cold-and-salt-stress-tolerance-mechanisms-in-banana
#2
Wei Hu, Zehong Ding, Weiwei Tie, Yan Yan, Yang Liu, Chunlai Wu, Juhua Liu, Jiashui Wang, Ming Peng, Biyu Xu, Zhiqiang Jin
The growth, development, and production of banana plants are constrained by multiple abiotic stressors. However, it remains elusive for the tolerance mechanisms of banana responding to multiple abiotic stresses. In this study, we found that Fen Jiao (FJ) was more tolerant to osmotic, cold, and salt stresses than BaXi Jiao (BX) by phenotypic and physiological analyses. Comparative transcriptomic analyses highlighted stress tolerance genes that either specifically regulated in FJ or changed more than twofold in FJ relative to BX after treatments...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223317/stabilized1-modulates-pre-mrna-splicing-for-thermotolerance
#3
Geundon Kim, Young-Hee Cho, Byeong-Ha Lee, Sang-Dong Yoo
High temperature stress often leads to differential RNA splicing, thus accumulating different types and/or amounts of mature mRNAs in eukaryotic cells. However, regulatory mechanisms underlying plant pre-mRNA splicing in the environmental stress conditions remain elusive. Herein, we describe that a U5-snRNP-interacting protein homolog STABILIZED1 (STA1) has pre-mRNA splicing activity for heat-inducible transcripts including HEAT STRESS TRANSCRIPTION FACTORS and various HEAT SHOCK PROTEINS for the establishment of heat stress tolerance in Arabidopsis thaliana...
February 21, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28222239/evolutionary-rescue-and-local-adaptation-under-different-rates-of-temperature-increase-a-combined-analysis-of-changes-in-phenotype-expression-and-genotype-frequency-in-paramecium-microcosms
#4
Joshua Killeen, Claire Gougat-Barbera, Sascha Krenek, Oliver Kaltz
Evolutionary Rescue (ER) occurs when populations, which have declined due to rapid environmental change, recover through genetic adaptation. The success of this process and the evolutionary trajectory of the population strongly depend on the rate of environmental change. Here we investigated how different rates of temperature increase (from 23°C to 32°C) affect population persistence and evolutionary change in experimental microcosms of the protozoan Paramecium caudatum. Consistent with theory on ER, we found that those populations experiencing the slowest rate of temperature increase were the least likely to become extinct and tended to be the best adapted to the new temperature environment...
February 21, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28219390/the-genome-landscape-of-indigenous-african-cattle
#5
Jaemin Kim, Olivier Hanotte, Okeyo Ally Mwai, Tadelle Dessie, Salim Bashir, Boubacar Diallo, Morris Agaba, Kwondo Kim, Woori Kwak, Samsun Sung, Minseok Seo, Hyeonsoo Jeong, Taehyung Kwon, Mengistie Taye, Ki-Duk Song, Dajeong Lim, Seoae Cho, Hyun-Jeong Lee, Duhak Yoon, Sung Jong Oh, Stephen Kemp, Hak-Kyo Lee, Heebal Kim
BACKGROUND: The history of African indigenous cattle and their adaptation to environmental and human selection pressure is at the root of their remarkable diversity. Characterization of this diversity is an essential step towards understanding the genomic basis of productivity and adaptation to survival under African farming systems. RESULTS: We analyze patterns of African cattle genetic variation by sequencing 48 genomes from five indigenous populations and comparing them to the genomes of 53 commercial taurine breeds...
February 20, 2017: Genome Biology
https://www.readbyqxmd.com/read/28217005/chromatin-based-epigenetic-regulation-of-plant-abiotic-stress-response
#6
Garima Pandey, Namisha Sharma, Pranav Pankaj Sahu, Manoj Prasad
Plants are continuously exposed to various abiotic and biotic factors limiting their growth and reproduction. In response, they need various sophisticated ways to adapt to adverse environmental conditions without compromising their proper development, reproductive success and eventually survival. This requires an intricate network to regulate gene expression at transcriptional and post-transcriptional levels, including epigenetic switches. Changes in chromatin modifications such as DNA and histone methylation have been observed in plants upon exposure to several abiotic stresses...
December 2016: Current Genomics
https://www.readbyqxmd.com/read/28216116/pain-sensitivity-and-pain-scoring-in-patients-with-morbid-obesity
#7
Bart Torensma, Linda Oudejans, Monique van Velzen, Dingeman Swank, Marieke Niesters, Albert Dahan
BACKGROUND: There are indications that pain perception is altered in patients with obesity, which complicates postoperative pain treatment. An essential part for adequate pain treatment is the capacity of the patient to grade pain. OBJECTIVES: The aim of this study was to identify the differences in pain perception and pain processing in patients with without obesity. SETTING: Dutch Obesity Clinic West; private practice and the Leiden University Medical Center, the Netherlands; university hospital...
January 10, 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28216106/metabolic-engineering-of-a-haploid-strain-derived-from-a-triploid-industrial-yeast-for-producing-cellulosic-ethanol
#8
Soo Rin Kim, Jeffrey M Skerker, In Iok Kong, Heejin Kim, Matthew J Maurer, Guo-Chang Zhang, Dairong Peng, Na Wei, Adam P Arkin, Yong-Su Jin
Many desired phenotypes for producing cellulosic biofuels are often observed in industrial Saccharomyces cerevisiae strains. However, many industrial yeast strains are polyploid and have low spore viability, making it difficult to use these strains for metabolic engineering applications. We selected the polyploid industrial strain S. cerevisiae ATCC 4124 exhibiting rapid glucose fermentation capability, high ethanol productivity, strong heat and inhibitor tolerance in order to construct an optimal yeast strain for producing cellulosic ethanol...
February 16, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28214356/comparative-analysis-of-the-transcriptional-responses-to-low-and-high-temperature-in-three-rice-planthopper-species
#9
Hai-Jian Huang, Jian Xue, Ji-Chong Zhuo, Ruo-Lin Cheng, Hai-Jun Xu, Chuan-Xi Zhang
The brown planthopper (Nilaparvata lugens, BPH), white-backed planthopper (Sogatella furcifera, WBPH), and small brown planthopper (Laodelphax striatellus, SBPH) are important rice pests in Asia. These three species differ in thermal tolerance and exhibit quite different migration and overwintering strategies. To understand the underlying mechanisms, we sequenced and compared the transcriptome of the three species under different temperature treatments. We found that metabolism-, exoskeleton-, and chemosensory-related genes were modulated...
February 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28199351/physiological-plasticity-related-to-zonation-affects-hsp70-expression-in-the-reef-building-coral-pocillopora-verrucosa
#10
Davide Poli, Elena Fabbri, Stefano Goffredo, Valentina Airi, Silvia Franzellitti
This study investigates for the first time the transcriptional regulation of a stress-inducible 70-kDa heat shock protein (hsp70) in the scleractinian coral Pocillopora verrucosa sampled at three locations and two depths (3 m and 12 m) in Bangka Island waters (North Sulawesi, Indonesia). Percentage of coral cover indicated reduced habitat suitability with depth and at the Tanjung Husi (TA) site, which also displayed relatively higher seawater temperatures. Expression of the P. verrucosa hsp70 transcript evaluated under field conditions followed a depth-related profile, with relatively higher expression levels in 3-m collected nubbins compared to the 12-m ones...
2017: PloS One
https://www.readbyqxmd.com/read/28199171/climate-adaptation-of-tropical-cattle
#11
W Barendse
There is sustained growth in the number of tropical cattle, which represent more than half of all cattle worldwide. By and large, most research in tropical areas is still focused on breeds of cattle, their particular advantages or disadvantages in tropical areas, and the tropical forages or feeds that could be usefully fed to them. A consistent issue for adaptation to climate is the heat of tropical environments. Changing the external characteristics of the animal, such as color and coat characteristics, is one way to adapt, and there are several major genes for these traits...
February 8, 2017: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/28194152/hsp65-producing-lactococcus-lactis-prevents-inflammatory-intestinal-disease-in-mice-by-il-10-and-tlr2-dependent-pathways
#12
Ana Cristina Gomes-Santos, Rafael Pires de Oliveira, Thaís Garcias Moreira, Archimedes Barbosa Castro-Junior, Bernardo Coelho Horta, Luísa Lemos, Leonardo Augusto de Almeida, Rafael Machado Rezende, Denise Carmona Cara, Sérgio Costa Oliveira, Vasco Ariston Carvalho Azevedo, Anderson Miyoshi, Ana Maria Caetano Faria
Heat shock proteins (Hsps) are highly expressed at all sites of inflammation. As they are ubiquitous and immunodominant antigens, these molecules represent good candidates for the therapeutic use of oral tolerance in autoimmune and chronic inflammatory diseases. Evidences from human and animal studies indicate that inflammatory bowel disease (IBD) results from uncontrolled inflammatory responses to intestinal microbiota. Hsps are immunodominant proteins expressed by several immune cells and by commensal bacteria...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28193450/heat-shock-70%C3%A2-kda-protein-cognate-5-involved-in-wssv-toleration-of-litopenaeus-vannamei
#13
Kai Yuan, Feng-Hua Yuan, Hong-Hui He, Hai-Tao Bi, Shao-Ping Weng, Jian-Guo He, Yi-Hong Chen
The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5...
February 10, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28193304/physiological-response-and-semen-quality-of-rabbit-bucks-supplemented-with-moringa-leaves-ethanolic-extract-during-summer-season
#14
N I El-Desoky, N M Hashem, A Elkomy, Z R Abo-Elezz
Exposure of rabbit bucks to summer heat stress reduces their homeostasis and semen quality leading to a temporal subfertility. The potentiality of ethanolic extract of Moringa oleifera leaves (M. oleifera ethanolic extract (MLEE)) to reduce negative impacts of heat stress on physiological and semen quality traits was investigated. A total of 28 adult V-line rabbit bucks were randomly distributed among four experimental groups of seven rabbits each. The first group received water (placebo) and served as a control (M0)...
February 14, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28193292/the-effect-of-elevated-temperatures-on-the-life-history-and-insecticide-resistance-phenotype-of-the-major-malaria-vector-anopheles-arabiensis-diptera-culicidae
#15
Shüné V Oliver, Basil D Brooke
BACKGROUND: Temperature plays a crucial role in the life history of insects. Recent climate change research has highlighted the importance of elevated temperature on malaria vector distribution. This study aims to examine the role of elevated temperatures on epidemiologically important life-history traits in the major malaria vector, Anopheles arabiensis. Specifically, the differential effects of temperature on insecticide-resistant and susceptible strains were examined. METHODS: Two laboratory strains of A...
February 14, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28190387/engineering-crops-for-future-a-phosphoproteomics-approach
#16
Vinay Kumar, Tushar Khare, Mansi Sharma, Shabir Hussain Wani
Abiotic stresses like salinity, drought, heat, metal, radiation and oxidative stress, and especially their combinations are major limiting factors for growth and productivity of the crops. Various molecular and biochemical processes governing the plant responses to abiotic stresses have often been investigated and hold the key for producing high-yielding and abiotic stress-tolerant crops. Plant responses to abiotic stresses are dynamic and intricate, and vary with type, level, and duration of the stress involved, as well as on the type of tissue under stress...
February 9, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/28188907/safety-pharmacokinetic-and-pharmacodynamic-study-in-healthy-subjects-of-oral-neo6860-a-modality-selective-trpv1-antagonist
#17
William Brown, Richard L Leff, Andrew Griffin, Stuart Hossack, Roxane Aubray, Philippe Walker, Dan A Chiche
: Most previous TRPV1 antagonist programs have been put on hold, mainly due on-target adverse events: hyperthermia and impaired noxious heat sensation. NEO6860 is a TRPV1 antagonist, blocking capsaicin activation of the target, with little or no effect against pH or heat activation. The hypothesis is that this pharmacological profile will translate into analgesia without undesired effects on the body temperature or heat-pain threshold. This phase I, double blind, placebo controlled, ascending dose study, included 64 subjects...
February 7, 2017: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28187140/diel-periodicity-of-drosophila-suzukii-diptera-drosophilidae-under-field-conditions
#18
Richard K Evans, Michael D Toews, Ashfaq A Sial
Drosophila suzukii Matsumura (Diptera: Drosophilidae), an economically important pest of blueberry and other thin-skinned fruits, persists and prolifically reproduces under seemingly lethal climatic conditions in the field. However, behavioral and physiological mechanisms employed by D. suzukii to tolerate such extreme climatic conditions in the field are unknown. The primary objective of this project was to investigate diel periodicity of D. suzukii and their reproductive success under field conditions as related by climatic factors such as temperature and relative humidity...
2017: PloS One
https://www.readbyqxmd.com/read/28187136/candidate-genes-and-molecular-markers-associated-with-heat-tolerance-in-colonial-bentgrass
#19
David Jespersen, Faith C Belanger, Bingru Huang
Elevated temperature is a major abiotic stress limiting the growth of cool-season grasses during the summer months. The objectives of this study were to determine the genetic variation in the expression patterns of selected genes involved in several major metabolic pathways regulating heat tolerance for two genotypes contrasting in heat tolerance to confirm their status as potential candidate genes, and to identify PCR-based markers associated with candidate genes related to heat tolerance in a colonial (Agrostis capillaris L...
2017: PloS One
https://www.readbyqxmd.com/read/28186132/bacterial-community-dynamics-are-linked-to-patterns-of-coral-heat-tolerance
#20
Maren Ziegler, Francois O Seneca, Lauren K Yum, Stephen R Palumbi, Christian R Voolstra
Ocean warming threatens corals and the coral reef ecosystem. Nevertheless, corals can be adapted to their thermal environment and inherit heat tolerance across generations. In addition, the diverse microbes that associate with corals have the capacity for more rapid change, potentially aiding the adaptation of long-lived corals. Here, we show that the microbiome of reef corals is different across thermally variable habitats and changes over time when corals are reciprocally transplanted. Exposing these corals to thermal bleaching conditions changes the microbiome for heat-sensitive corals, but not for heat-tolerant corals growing in habitats with natural high heat extremes...
February 10, 2017: Nature Communications
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