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https://www.readbyqxmd.com/read/29146915/transcriptional-profiling-provides-new-insights-into-the-role-of-nitric-oxide-in-enhancing-ganoderma-oregonense-resistance-to-heat-stress
#1
Cheng Chen, Qiang Li, Qiangfeng Wang, Daihua Lu, Hong Zhang, Jian Wang, Rongtao Fu
Ganoderma is well known for its use in traditional Chinese medicine and is widely cultivated in China, Korea, and Japan. Increased temperatures associated with global warming are negatively influencing the growth and development of Ganoderma. Nitric oxide is reported to play an important role in alleviating fungal heat stress (HS). However, the transcriptional profiling of Ganoderma oregonense in response to HS, as well as the transcriptional response regulated by NO to cope with HS has not been reported. We used RNA-Seq technology to generate large-scale transcriptome data from G...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146501/antibacterial-activity-and-probiotic-characterization-of-autochthonous-paenibacillus-polymyxa-isolated-from-anabas-testudineus-bloch-1792
#2
Sebastian J Midhun, Sahadevan Neethu, A Vysakh, Damodaran Arun, E K Radhakrishnan, Mathew Jyothis
Microbial fish pathogens are prevalent in aquaculture. Control of Bacterial fish pathogens is important and bio control of pathogenic bacteria is a novel field of study. The aim of this study was to evaluate the antagonistic activity of bacteria isolated from Anabas testudineus against potent fish pathogens. The cellular components/preparations and filtered cell free culture supernatants were effective against six fish pathogens. Altogether 110 strains were isolated from fish proximal and distal intestine, out of which 10 strains were selected through well diffusion method...
November 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29141194/marginal-stability-drives-irreversible-unfolding-of-large-multi-domain-family-3-glycosylhydrolases-from-thermo-tolerant-yeast
#3
Mohammad Asif Shah, Saroj Mishra, Tapan Kumar Chaudhuri
Protein folding is an extremely complex and fast, yet perfectly defined process, involving interplay of many intra and inter-molecular forces. In vitro, these molecular interactions are reversible for many proteins e.g., smaller and monomeric, organized into single domains. However, refolding of larger multi-domain/multimeric proteins is much more complicated, proceeds in a hierarchal way and is often irreversible. In a comparative study on two large, multi-domain and multimeric isozymes, β -glucosidase I (BGLI) and β-glucosidase II (BGLII) from Pichia etchellsii, we studied spontaneous and assisted refolding under three denaturing conditions viz...
November 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29140896/cold-water-ingestion-improves-exercise-tolerance-of-heat-sensitive-people-with-ms
#4
Georgia K Chaseling, Davide Filingeri, Michael Barnett, Phu Hoang, Scott L Davis, Ollie Jay
PURPOSE: Heat intolerance commonly affects the exercise capacity of people with multiple sclerosis (MS) during bouts of hot weather. Cold-water ingestion is a simple cooling strategy but its efficacy for prolonging exercise capacity with MS remains undetermined. We sought to identify whether cold-water ingestion blunts exercise-induced rises in body temperature and improves exercise tolerance in heat-sensitive individuals with MS. METHODS: On two separate occasions, 20 participants (10 relapsing-remitting MS (EDSS: 1-5); 10 age-matched healthy controls (CON)) cycled at ~40% VO2max at 30°C, 30%RH until volitional exhaustion (or a maximum of 60 min)...
November 15, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29137993/characterization-of-cold-adapted-and-ethanol-tolerant-%C3%AE-glucosidase-from-bacillus-cellulosilyticus-and-its-application-for-directed-hydrolysis-of-cellobiose-to-ethanol
#5
Jian Wu, Alei Geng, Rongrong Xie, Hongcheng Wang, Jianzhong Sun
A β-glucosidase gene from Bacillus cellulosilyticus was expressed in Escherichia coli BL21 and characterized. The recombinant enzyme (BcBgl1A) showed the optimal activity at 40°C, and the half-life of BcBgl1A activity at 40 °C was 24 h. BcBgl1A was sensitive to heat and exhibited obvious cold-adapted activity, retained 27.2% and 46.5% of the optimal activity at 4 and 20°C, respectively. The best activity was observed at pH 7.0 and stable over the range of pH 6.0-8.0. The activity was significantly enhanced by Fe(2+), Fe(3+) and Mn(2+), also showed a high level of NaCl tolerance...
November 11, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29131867/differential-transcriptome-analysis-reveals-genes-related-to-cold-tolerance-in-seabuckthorn-carpenter-moth-eogystia-hippophaecolus
#6
Mingming Cui, Ping Hu, Tao Wang, Jing Tao, Shixiang Zong
Seabuckthorn carpenter moth, Eogystia hippophaecolus (Lepidoptera: Cossidae), is an important pest of sea buckthorn (Hippophae rhamnoides), which is a shrub that has significant ecological and economic value in China. E. hippophaecolus is highly cold tolerant, but limited studies have been conducted to elucidate the molecular mechanisms underlying its cold resistance. Here we sequenced the E. hippophaecolus transcriptome using RNA-Seq technology and performed de novo assembly from the short paired-end reads...
2017: PloS One
https://www.readbyqxmd.com/read/29127509/heart-rate-variability-and-plasma-nephrines-in-the-evaluation-of-heat-acclimatisation-status
#7
Major Michael John Stacey, S K Delves, D R Woods, S E Britland, L Macconnachie, A J Allsopp, S J Brett, J L Fallowfield, C J Boos
PURPOSE: Heat adaptation (HA) is critical to performance and health in a hot environment. Transition from short-term heat acclimatisation (STHA) to long-term heat acclimatisation (LTHA) is characterised by decreased autonomic disturbance and increased protection from thermal injury. A standard heat tolerance test (HTT) is recommended for validating exercise performance status, but any role in distinguishing STHA from LTHA is unreported. The aims of this study were to (1) define performance status by serial HTT during structured natural HA, (2) evaluate surrogate markers of autonomic activation, including heart rate variability (HRV), in relation to HA status...
November 10, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29126027/strong-differences-between-two-congeneric-species-in-sensitivity-to-pesticides-in-a-warming-world
#8
Lin Op de Beeck, Julie Verheyen, Robby Stoks
To predict the impact of pesticides in a warming world we need to know how species differ in the interaction pathways between pesticides and warming. Trait-based approaches have been successful in identifying the 'pace of life' and body size as predictors of sensitivity to pesticides among distantly related species. However, it remains to be tested whether these traits allow predicting differences in sensitivity to pesticides between closely related species, and in the strength of the interaction pathways between pesticides and warming...
November 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29124652/red-shift-in-the-spectrum-of-a-chlorophyll-species-is-essential-for-the-drought-induced-dissipation-of-excess-light-energy-in-a-poikilohydric-moss-bryum-argenteum
#9
Yutaka Shibata, Ahmed Mohamed, Koichiro Taniyama, Kentaro Kanatani, Makiko Kosugi, Hiroshi Fukumura
Some mosses are extremely tolerant of drought stress. Their high drought tolerance relies on their ability to effectively dissipate absorbed light energy to heat under dry conditions. The energy dissipation mechanism in a drought-tolerant moss, Bryum argenteum, has been investigated using low-temperature picosecond time-resolved fluorescence spectroscopy. The results are compared between moss thalli samples harvested in Antarctica and in Japan. Both samples show almost the same quenching properties, suggesting an identical drought tolerance mechanism for the same species with two completely different habitats...
November 9, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/29124510/lps-ameliorates-renal-ischemia-reperfusion-injury-via-hsp27-up-regulation
#10
Kang He, Lei Xia, Jianjun Zhang
PURPOSE: We have recently reported lipopolysaccharide (LPS) pretreatment attenuated renal ischemia/reperfusion injury (IRI), but the exact mechanism remains to be well elucidated. It was reported that heat shock protein (Hsp) 27 was up-regulated after administration of LPS, but whether a direct link existed between Hsp27 up-regulation and LPS-induced protection against renal IRI is still unknown. METHODS: Mice were exposed to IRI or sham procedure, with pretreatment of LPS or not...
November 9, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29124059/evaluation-of-three-hydration-strategies-in-detection-dogs-working-in-a-hot-environment
#11
Cynthia M Otto, Elizabeth Hare, Jess L Nord, Shannon M Palermo, Kathleen M Kelsey, Tracy A Darling, Kasey Schmidt, Destiny Coleman
Physical activity in hot environments can increase the risk of heat stress or heat stroke in dogs. Heat tolerance is influenced by acclimatization to the environment, physical fitness, and hydration state. Three common strategies to promote hydration in working dogs are free access to water (W), oral electrolyte solutions (OESs), and administration of subcutaneous fluids (SQs). None of these methods have been compared for safety or efficacy in a working environment. In a cross-over design, seven vehicle-screening canines were randomly assigned to each of the three hydration strategies during working shifts at the Sarita, TX checkpoint...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29123535/trait-and-marker-associations-in-oryza-nivara-and-o-rufipogon-derived-rice-lines-under-two-different-heat-stress-conditions
#12
V Vishnu Prasanth, M Suchandranath Babu, Ramana K Basava, V G N Tripura Venkata, Satendra K Mangrauthia, S R Voleti, Sarla Neelamraju
Wild species and derived introgression lines (ILs) are a good source of genes for improving complex traits such as heat tolerance. The effect of heat stress on 18 yield traits was studied in four treatments in two seasons, under field conditions by subjecting 37 ILs and recurrent parents Swarna and KMR3, N22 mutants, and wild type and 2 improved rice cultivars to heat stress using polycover house method in wet season and late sowing method in dry season. Normal grown unstressed plants were controls. Both correlation and path coefficient analysis showed that the major contributing traits for high yield per plant (YPP) under heat stress conditions were tiller number, secondary branches in panicle, filled grain number, and percent spikelet fertility...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29123532/food-legumes-and-rising-temperatures-effects-adaptive-functional-mechanisms-specific-to-reproductive-growth-stage-and-strategies-to-improve-heat-tolerance
#13
REVIEW
Kumari Sita, Akanksha Sehgal, Bindumadhava HanumanthaRao, Ramakrishnan M Nair, P V Vara Prasad, Shiv Kumar, Pooran M Gaur, Muhammad Farooq, Kadambot H M Siddique, Rajeev K Varshney, Harsh Nayyar
Ambient temperatures are predicted to rise in the future owing to several reasons associated with global climate changes. These temperature increases can result in heat stress- a severe threat to crop production in most countries. Legumes are well-known for their impact on agricultural sustainability as well as their nutritional and health benefits. Heat stress imposes challenges for legume crops and has deleterious effects on the morphology, physiology, and reproductive growth of plants. High-temperature stress at the time of the reproductive stage is becoming a severe limitation for production of grain legumes as their cultivation expands to warmer environments and temperature variability increases due to climate change...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29123529/t-cell-mediated-chronic-inflammatory-diseases-are-candidates-for-therapeutic-tolerance-induction-with-heat-shock-proteins
#14
REVIEW
Ariana Barbera Betancourt, Qingkang Lyu, Femke Broere, Alice Sijts, Victor P M G Rutten, Willem van Eden
Failing immunological tolerance for critical self-antigens is the problem underlying most chronic inflammatory diseases of humans. Despite the success of novel immunosuppressive biological drugs, the so-called biologics, in the treatment of diseases such rheumatoid arthritis (RA) and type 1 diabetes, none of these approaches does lead to a permanent state of medicine free disease remission. Therefore, there is a need for therapies that restore physiological mechanisms of self-tolerance. Heat shock proteins (HSPs) have shown disease suppressive activities in many models of experimental autoimmune diseases through the induction of regulatory T cells (Tregs)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29121266/stress-inducible-galactinol-synthase-of-chickpea-cagols-implicates-in-heat-and-oxidative-stress-tolerance-through-reducing-stress-induced-excessive-reactive-oxygen-species-accumulation
#15
Prafull Salvi, Nitin Uttam Kamble, Manoj Majee
Raffinose Family Oligosaccharides (RFOs) participates in various aspects of plant physiology and galactinol synthase (GolS, EC 2.4.1.123) catalyzes the key step of RFO biosynthesis. Stress induced accumulation of RFOs particularly galactinol and raffinose has been reported in few plants; however their precise role and mechanistic insight in stress adaptation remain elusive. In present study, we have shown that the GolS activity as well as galactinol and raffinose content are significantly increased in response to various abiotic stresses in chickpea...
November 7, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29120390/functional-characterization-of-waterlogging-and-heat-stresses-tolerance-gene-pyruvate-decarboxylase-2-from-actinidia-deliciosa
#16
Hui-Ting Luo, Ji-Yu Zhang, Gang Wang, Zhan-Hui Jia, Sheng-Nan Huang, Tao Wang, Zhong-Ren Guo
A previous report showed that both Pyruvatedecarboxylase (PDC) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit AdPDC1 gene was required during waterlogging, but might not be required during other environmental stresses. Here, the function of another PDC gene, named AdPDC2, was analyzed. The expression of the AdPDC2 gene was determined using qRT-PCR, and the results showed that the expression levels of AdPDC2 in the reproductive organs were much higher than those in the nutritive organs...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29118776/design-of-a-custom-rt-qpcr-array-for-assignment-of-abiotic-stress-tolerance-in-traditional-portuguese-grapevine-varieties
#17
Luísa C Carvalho, Marília Silva, João L Coito, Margarida P Rocheta, Sara Amâncio
Widespread agricultural losses attributed to drought, often combined with high temperatures, frequently occur in the field, namely in Mediterranean climate areas, where the existing scenarios for climate change indicate an increase in the frequency of heat waves and severe drought events in summer. Grapevine (Vitis vinifera L.) is the most cultivated fruit species in the world and the most valuable one and is a traditional Mediterranean species. Currently, viticulture must adjust to impending climate changes that are already pushing vine-growers toward the use of ancient and resilient varieties...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29118239/a-positive-genetic-correlation-between-hypoxia-tolerance-and-heat-tolerance-supports-a-controversial-theory-of-heat-stress
#18
Collin Teague, Jacob P Youngblood, Kinley Ragan, Michael J Angilletta, John M VandenBrooks
We used quantitative genetics to test a controversial theory of heat stress, in which animals overheat when the demand for oxygen exceeds the supply. This theory, referred to as oxygen- and capacity-limited thermal tolerance, predicts a positive genetic correlation between hypoxia tolerance and heat tolerance. We demonstrate the first genetic correlation of this kind in a model organism, Drosophila melanogaster Genotypes more likely to fly under hypoxic stress (12% O2) were also more likely to fly under heat stress (39°C)...
November 2017: Biology Letters
https://www.readbyqxmd.com/read/29113889/production-optimization-purification-and-characterization-of-a-heat-tolerant-acidic-pectinase-from-bacillus-sp-zj1407
#19
Ping Yu, Chaochao Xu
Medium compositions for a heat-tolerant acidic pectinase production from Bacillus sp. ZJ1407 were optimized via response surface methodology (RSM) and its enzymatic properties were investigated. A 2-level factorial design was used to estimate the main effect of factors, and to screen the significant factors. A central composite design was used to find out the optimal concentrations of screened key factors. Lactose, tryptone and (NH4)2SO4 were found to have a significant influence on the pectinase activity (p <0...
November 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29110450/three-dimensional-framework-of-graphene-nanomeshes-shell-co3o4-synthesized-as-superior-bifunctional-electrocatalyst-for-zinc-air-batteries
#20
Congwei Wang, Zheng Zhao, Xiaofeng Li, Rui Yan, Jie Wang, Anni Li, Xiaoyong Duan, Junying Wang, Yong Liu, Junzhong Wang
The synthesis of durable and low-cost electrocatalyst is crucial but challenging. Here, we developed a one-pot pyrolysis approach toward the preparation of heteroatom-doped hierarchical porous three-dimensional (3D) graphene frameworks decorated with multilayer graphene shell-coated cobalt oxide nanocrystal. Large literal sheet size of graphene nanomeshes may stimulate rapid thermolysis with cobalt-oleate complex to form Co3O4 nanocrystals and in situ growth of multilayer graphene coating co-doped by boron and nitrogen with controlling heating rate up to 600 °C...
November 14, 2017: ACS Applied Materials & Interfaces
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