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https://www.readbyqxmd.com/read/29332191/genome-wide-characterization-of-differentially-expressed-genes-provides-insights-into-regulatory-network-of-heat-stress-response-in-radish-raphanus-sativus-l
#1
Ronghua Wang, Yi Mei, Liang Xu, Xianwen Zhu, Yan Wang, Jun Guo, Liwang Liu
Heat stress (HS) causes detrimental effects on plant morphology, physiology, and biochemistry that lead to drastic reduction in plant biomass production and economic yield worldwide. To date, little is known about HS-responsive genes involved in thermotolerance mechanism in radish. In this study, a total of 6600 differentially expressed genes (DEGs) from the control and Heat24 cDNA libraries of radish were isolated by high-throughput sequencing. With Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, some genes including MAPK, DREB, ERF, AP2, GST, Hsf, and Hsp were predominantly assigned in signal transductions, metabolic pathways, and biosynthesis and abiotic stress-responsive pathways...
January 13, 2018: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/29331284/effects-of-an-experimental-heat-wave-on-fatty-acid-composition-in-two-mediterranean-seagrass-species
#2
Pedro Beca-Carretero, Freddy Guihéneuf, Lázaro Marín-Guirao, Jaime Bernardeau-Esteller, Rocío García-Muñoz, Dagmar B Stengel, Juan M Ruiz
Global warming is emerging as one of the most critical threats to terrestrial and marine species worldwide. This study assessed the effects of simulated warming events in culture on two seagrass species, Posidonia oceanica and Cymodocea nodosa, which play a key role in coastal ecosystems of the Mediterranean Sea. Changes in fatty acids as key metabolic indicators were assessed in specimens from two geographical populations of each species adapted to different in situ temperature regimes. Total fatty acid (TFA) content and composition were compared in C...
January 10, 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29330772/rice-wrky11-plays-a-role-in-pathogen-defense-and-drought-tolerance
#3
Heyoung Lee, Jooyoung Cha, Changhyun Choi, Naeyoung Choi, Hyun-So Ji, Sang Ryeol Park, Seungbum Lee, Duk-Ju Hwang
BACKGROUND: Plants are frequently subjected to abiotic and biotic stresses, and WRKY proteins play a pivotal role in the response to such stress. OsWRKY11 is induced by pathogens, drought, and heat, suggesting a function in biotic and abiotic stress responses. RESULTS: This study identified OsWRKY11, a member of WRKY group IIc. It is a transcriptional activator that localized to the nucleus. Ectopic expression of OsWRKY11 resulted in enhanced resistance to a bacterial pathogen, Xanthomonas oryzae pv...
January 12, 2018: Rice
https://www.readbyqxmd.com/read/29330650/a-cold-adapted-endoglucanase-from-camel-rumen-with-high-catalytic-activity-at-moderate-and-low-temperatures-an-anomaly-of-truly-cold-adapted-evolution-in-a-mesophilic-environment
#4
Kamran Khalili Ghadikolaei, Javad Gharechahi, Kamahldin Haghbeen, Kambiz Akbari Noghabi, Ghasem Hosseini Salekdeh, Hossein Shahbani Zahiri
Endoglucanases are important enzymes in plant biomass degradation. They have current and potential applications in various industrial sectors including human and animal food processing, textile, paper, and renewable biofuel production. It is assumed that the cold-active endoglucanases, with high catalytic rates in moderate and cold temperatures, can improve the cost-effectiveness of industrial processes by lowering the need for heating and, thus, energy consumption. In this study, the endoglucanase CelCM3 was procured from a camel rumen metagenome via gene cloning and expression in Escherichia coli BL21 (DE3)...
January 12, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29323664/on-demand-synthesis-of-organozinc-halides-under-continuous-flow-conditions
#5
Mateo Berton, Lena Huck, Jesús Alcázar
Organozinc reagents are versatile building blocks for introducing C(sp2)-C(sp3) and C(sp3)-C(sp3) bonds into organic structures. However, despite their ample synthetic versatility and broad functional group tolerance, the use of organozinc reagents in the laboratory is limited because of their instability, exothermicity and water sensitivity, as well as their labor-intensive preparation. Herein, we describe an on-demand synthesis of these useful reagents under continuous flow conditions, overcoming these primary limitations and supporting widespread adoption of these reagents in synthetic organic chemistry...
January 2018: Nature Protocols
https://www.readbyqxmd.com/read/29316302/oxygen-supply-did-not-affect-how-lizards-responded-to-thermal-stress
#6
Agustin Camacho Guerrero, John M VandenBrooks, Angela Riley, Rory S Telemeco, Michael J Angilletta
Zoologists rely on mechanistic niche models of behavioral thermoregulation to understand how animals respond to climate change. These models predict that species will need to disperse to higher altitudes to persist in a warmer world. However, thermal stress and thus thermoregulatory behavior may depend on atmospheric oxygen as well as environmental temperatures. Severe hypoxia causes animals to prefer lower body temperatures, which could be interpreted as evidence that oxygen supply limits heat tolerance. Such a constraint could prevent animals from successfully dispersing to high elevations during climate change...
January 9, 2018: Integrative Zoology
https://www.readbyqxmd.com/read/29314047/plasticity-of-photosynthetic-heat-tolerance-in-plants-adapted-to-thermally-contrasting-biomes
#7
Lingling Zhu, Keith J Bloomfield, Charles H Hocart, John J G Egerton, Odhran S O'Sullivan, Aurore Penillard, Lasantha K Weerasinghe, Owen K Atkin
In many biomes, plants are subject to heat-waves, potentially causing irreversible damage to the photosynthetic apparatus. Field surveys have documented global, temperature-dependent patterns in photosynthetic heat tolerance (PHT ); however, it remains unclear if these patterns reflect acclimation in PHT or inherent differences among species adapted to contrasting habitats. To address these unknowns, we quantified seasonal variations in Tcrit (high temperature where minimal chlorophyll-a fluorescence rises rapidly, reflecting disruption to photosystem II) in 62 species native to six sites from five thermally-contrasting biomes across Australia...
January 5, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29313416/dicot-specific-atg8-interacting-ati3-proteins-interact-with-conserved-ubac2-proteins-and-play-critical-roles-in-plant-stress-responses
#8
Jie Zhou, Zhe Wang, Xiaoting Wang, Xifeng Li, Zhenchao Zhang, Baofang Fan, Cheng Zhu, Zhixiang Chen
Selective macroautophagy/autophagy targets specific cargo by autophagy receptors through interaction with ATG8 (autophagy-related protein 8)/MAP1LC3 (microtubule associated protein 1 light chain 3) for degradation in the vacuole. Here, we report the identification and characterization of 3 related ATG8-interacting proteins (AT1G17780/ATI3A, AT2G16575/ATI3B and AT1G73130/ATI3C) from Arabidopsis. ATI3 proteins contain a WxxL LC3-interacting region (LIR) motif at the C terminus required for interaction with ATG8...
January 9, 2018: Autophagy
https://www.readbyqxmd.com/read/29306198/a-glyphosate-based-herbicide-reduces-fertility-embryonic-upper-thermal-tolerance-and-alters-embryonic-diapause-of-the-threatened-annual-fish-austrolebias-nigrofasciatus
#9
Yuri Dornelles Zebral, Luize Real Lansini, Patrícia Gomes Costa, Mauricio Roza, Adalto Bianchini, Ricardo Berteaux Robaldo
Roundup is the most popular glyphosate-based herbicide (GBH) worldwide. These formulations kill a wide range of plants. Despite that, non-target species can be jeopardized by GBH, such as the annual fish Austrolebias nigrofasciatus. This species occurs in wetlands that dries annually. Key-adaptations permit them to live in such harsh habitats, e. i. Elevated fertility, drought-tolerant diapausing embryos and elevated thermal tolerance. We aimed to evaluate acute (96 h) effects of Roundup exposure (0.36 or 3...
January 2, 2018: Chemosphere
https://www.readbyqxmd.com/read/29305989/the-immune-response-of-mytilus-edulis-hemocytes-exposed-to-vibrio-splendidus-lgp32-strain-a-transcriptomic-attempt-at-identifying-molecular-actors
#10
Marion Tanguy, Sophie Gauthier-Clerc, Jocelyne Pellerin, Jean-Michel Danger, Ahmed Siah
The marine mussel Mytilus edulis, tolerant to a wide range of environmental changes, combines a key role as a sentinel species for environmental monitoring programs and a significant economic importance. Mortality events caused by infective agents and parasites have not been described in mussels, which suggests an efficient immune system. This study aims at identifying the molecular mechanisms involved in the early immune responses M. edulis' hemocytes challenged with Vibrio splendidus LGP32 strain during 2, 4 and 6 h...
January 3, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29302276/haemoglobin-mediated-response-to-hyper-thermal-stress-in-the-keystone-species-daphnia-magna
#11
Maria Cuenca Cambronero, Bettina Zeis, Luisa Orsini
Anthropogenic global warming has become a major geological and environmental force driving drastic changes in natural ecosystems. Due to the high thermal conductivity of water and the effects of temperature on metabolic processes, freshwater ecosystems are among the most impacted by these changes. The ability to tolerate changes in temperature may determine species long-term survival and fitness. Therefore, it is critical to identify coping mechanisms to thermal and hyper-thermal stress in aquatic organisms...
January 2018: Evolutionary Applications
https://www.readbyqxmd.com/read/29301684/the-effect-of-acclimation-on-heat-tolerance-of-lasioderma-serricorne-fabricius-coleoptera-anobiidae
#12
Meng Li, Xiao-Juan Li, Jian-Hua Lü, Ming-Fei Huo
Lasioderma serricorne (Fabricius) (Coleoptera: Anobiidae) is an important economic insect of various stored products worldwide. With the restricted use of methyl bromide and the increasing negative effects of repeated use of phosphine fumigation, heat treatment becomes the main approach to control stored-product insects. We investigated effect of the acclimation to sublethal high temperature of 36°C and 42°C for different times on heat tolerance of L. serricorne adults, pupae, larvae, and eggs to lethal high temperature of 50°C in laboratory...
January 2018: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29301249/candidate-genes-and-molecular-markers-correlated-to-physiological-traits-for-heat-tolerance-in-fine-fescue-cultivars
#13
Yi Xu, Jinyu Wang, Stacy A Bonos, William A Meyer, Bingru Huang
Heat stress is one of the major abiotic factors limiting the growth of cool-season grass species during summer season. The objectives of this study were to assess genetic variations in the transcript levels of selected genes in fine fescue cultivars differing in heat tolerance, and to identify single nucleotide polymorphism (SNP) markers associated with candidate genes related to heat tolerance. Plants of 26 cultivars of five fine fescue species (Festuca spp.) were subjected to heat stress (38/33 °C, day/night temperature) in controlled environmental growth chambers...
January 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29300786/signatures-of-positive-selection-in-african-butana-and-kenana-dairy-zebu-cattle
#14
Hussain Bahbahani, Bashir Salim, Faisal Almathen, Fahad Al Enezi, Joram M Mwacharo, Olivier Hanotte
Butana and Kenana are two types of zebu cattle found in Sudan. They are unique amongst African indigenous zebu cattle because of their high milk production. Aiming to understand their genome structure, we genotyped 25 individuals from each breed using the Illumina BovineHD Genotyping BeadChip. Genetic structure analysis shows that both breeds have an admixed genome composed of an even proportion of indicine (0.75 ± 0.03 in Butana, 0.76 ± 0.006 in Kenana) and taurine (0.23 ± 0.009 in Butana, 0.24 ± 0.006 in Kenana) ancestries...
2018: PloS One
https://www.readbyqxmd.com/read/29299523/clinical-feasibility-study-of-protrach-dualcare-a-new-speaking-valve-with-heat-and-moisture-exchanger-for-tracheotomized-patients
#15
B J de Kleijn, C J van As-Brooks, J Wedman, B F A M van der Laan
Objective: The aim of this study was to evaluate the clinical feasibility of the ProTrach DualCare (Atos Medical, Hörby, Sweden), a device combining a hands-free speaking valve and a Heat and Moisture Exchanger (HME) for tracheotomized patients. Study Design: A non-randomized, prospective single center feasibility study. Methods: Sixteen adult tracheotomized patients were included. Participants were asked to test the DualCare for two weeks while continuing their normal activities...
December 2017: Laryngoscope Investigative Otolaryngology
https://www.readbyqxmd.com/read/29299265/effects-of-temperature-and-drought-on-early-life-stages-in-three-species-of-butterflies-mortality-of-early-life-stages-as-a-key-determinant-of-vulnerability-to-climate-change
#16
Michael Klockmann, Klaus Fischer
Anthropogenic climate change poses substantial challenges to biodiversity conservation. Well-documented responses include phenological and range shifts, and declines in cold but increases in warm-adapted species. Thus, some species will suffer while others will benefit from ongoing change, although the biological features determining the prospects of a given species under climate change are largely unknown. By comparing three related butterfly species of different vulnerability to climate change, we show that stress tolerance during early development may be of key importance...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29298355/functional-regions-of-hpaxm-as-elicitors-with-specific-heat-tolerance-induce-the-hypersensitive-response-or-plant-growth-promotion-in-nonhost-plants
#17
Yue Liu, Xiaoyun Zhou, Wenbo Liu, Xiaohui Xiong, Chuyang Lv, Xiang Zhou, Weiguo Miao
HpaXm produced by the cotton leaf blight bacterium Xanthomonas citri subsp. malvacearum is a novel harpin elicitor of the induced hypersensitive response (HR) in tobacco. We investigated whether fragments of HpaXm, compared with fragments of Hpa1Xoo, are sufficient for HR or plant growth promotion (PGP) elicitation using four synthetic peptides (HpaXm35-51, HpaXm10-39, Hpa1Xoo36-52 and Hpa1Xoo10-40). We also heated the fragments to determine the heat tolerance of the functional fragments. HpaXm35-51 and Hpa1Xoo36-52 induced hypersensitive response (HR)...
2018: PloS One
https://www.readbyqxmd.com/read/29297201/geographic-variation-in-pollen-color-is-associated-with-temperature-stress
#18
Matthew H Koski, Laura F Galloway
The evolution of flower color, especially petal pigmentation, has received substantial attention. Less understood is the evolutionary ecology of pollen pigmentation, though it varies among and within species and its biochemical properties affect pollen viability. We characterize the distribution of pollen color across 24 populations of the North American herb Campanula americana, and assess the degree to which this variation is genetically based. We identify abiotic factors that covary with pollen color and test whether germination of light and dark pollen is differentially affected by variable temperature and UV...
January 3, 2018: New Phytologist
https://www.readbyqxmd.com/read/29288744/metabolic-suppression-in-the-pelagic-crab-pleuroncodes-planipes-in-oxygen-minimum-zones
#19
Brad A Seibel, Bryan E Luu, Shannon N Tessier, Trisha Towanda, Kenneth B Storey
The pelagic red crab, Pleuroncodes planipes, is abundant throughout the Eastern Tropical Pacific in both benthic and pelagic environments to depths of several hundred meters. The oxygen minimum zones in this region reaches oxygen levels as low as 0.1kPa at depths within the crabs vertical range. Crabs maintain aerobic metabolism to a critical PO2 of ~0.27±0.2kPa (10°C), in part by increasing ventilation as oxygen declines. At subcritical oxygen levels, they enhance anaerobic ATP production slightly as indicated by modest increases in lactate levels...
December 27, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29285618/oryza-sativa-salt-induced-ring-e3-ligase-2-ossirp2-acts-as-a-positive-regulator-of-transketolase-in-plant-response-to-salinity-and-osmotic-stress
#20
Sandeep Chapagain, Yong Chan Park, Ju Hee Kim, Cheol Seong Jang
A rice gene (OsSIRP2) encoding the RING Ub E3 ligase was highly induced under salinity stress and physically interacted with a transketolase (OsTKL1). Overexpression of OsSIRP2 conferred salinity and osmotic stress tolerance in plants. The RING E3 ligases play a vital role in post transitional modification through ubiquitination-mediated protein degradation that mediate plants responses during abiotic stresses and signal transduction. In this study, we report an Oryza sativa salt induced Really Interesting New Gene (RING) finger protein 2 gene (OsSIRP2) and elucidate its role under salinity and osmotic stress...
December 28, 2017: Planta
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