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https://www.readbyqxmd.com/read/28645157/secretory-iga-response-against-pseudomonas-aeruginosa-in-the-upper-airways-and-the-link-with-chronic-lung-infection-in-cystic-fibrosis
#1
Renan Mauch, Claudio Rossi, Talita Aiello, José Ribeiro, Antonio Ribeiro, Niels Høiby, Carlos Levy
We assessed the diagnostic ability of an ELISA test for measurement of specific secretory IgA (sIgA) in saliva to identify Cystic Fibrosis (CF) patients with P. aeruginosa chronic lung infection and intermittent lung colonization. A total of 102 Brazilian CF patients and 53 healthy controls were included. Specific serum IgG response was used as a surrogate to distinguish CF patients according to their P. aeruginosa colonization/infection status. The rate of sIgA positivity was 87.1% in CF chronically infected patients (Median value = 181...
June 22, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28633646/role-of-serotonin-on-the-intestinal-mucosal-immune-response-to-stress-induced-diarrhea-in-weaning-mice
#2
Yulan Dong, Yanan Han, Zixu Wang, Zhuoming Qin, Chenyu Yang, Jing Cao, Yaoxing Chen
BACKGROUND: During weaning, babies and young animal often experience diarrhea from food intolerance and/or decreasing levels of maternal antibodies, and diarrhea tends to be particularly severe during the early-weaned period, which often exhibits an underdeveloped immune system, a disturbed gut environment and results in nutrient malabsorption and dehydration. It was deduced that neuroendocrine might have close relation with diarrhea, especially 5-HT. METHODS: To explore the role of serotonin (5-HT) in weaning mice subjected to stress-induced diarrhea, 21-day-old weaned mice were divided into the following groups: control group, stress-induced diarrhea group (restrained by binding the hind limbs and intragastric administration of folium sennae with 0...
June 21, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28630108/inducing-mucosal-iga-a-challenge-for-vaccine-adjuvants-and-delivery-systems
#3
REVIEW
Prosper N Boyaka
Mucosal IgA or secretory IgA (SIgA) are structurally equipped to resist chemical degradation in the harsh environment of mucosal surfaces and enzymes of host or microbial origin. Production of SIgA is finely regulated, and distinct T-independent and T-dependent mechanisms orchestrate Ig α class switching and SIgA responses against commensal and pathogenic microbes. Most infectious pathogens enter the host via mucosal surfaces. To provide a first line of protection at these entry ports, vaccines are being developed to induce pathogen-specific SIgA in addition to systemic immunity achieved by injected vaccines...
July 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28625525/recombinant-lactobacillus-casei-expressing-clostridium-perfringens-toxoids-%C3%AE-%C3%AE-2-%C3%AE%C2%B5-and-%C3%AE-1-gives-protection-against-clostridium-perfringens-in-rabbits
#4
Li Zhao, Zhihou Guo, Jiali Liu, Zi Wang, Ruichong Wang, Yijing Li, Li Wang, Yigang Xu, Lijie Tang, Xinyuan Qiao
The present study used Lactobacillus casei ATCC 393 as antigen delivery system to express C. perfringens toxoids α-β2-ε-β1 to construct the recombination Lactobacillus casei pPG-2-α-β2-ε-β1/L. casei 393. After being induced by 1% xylose, the specificity and integrity of recombinant strain were determined by Western-blotting. Rabbits as native animal model were immunized orally with pPG-2-α-β2-ε-β1/L. casei 393 and the titers of specific IgG and sIgA were determined by ELISA. The result showed that oral administration with the recombinants could elicit both local mucosal and systemic immune responses...
June 15, 2017: Vaccine
https://www.readbyqxmd.com/read/28622869/brucella-lipopolysaccharide-reinforced-salmonella-delivering-brucella-immunogens-protects-mice-against-virulent-challenge
#5
Jonathan Lalsiamthara, John Hwa Lee
Intracellular pathogen Salmonella exhibits natural infection broadly analogous to Brucella, this phenomenon makes Salmonella a pragmatic choice for an anti-Brucella vaccine delivery platform. In this study we developed and formulated a combination of four attenuated Salmonella Typhimurium live vector strains delivering heterologous Brucella antigens (rBs), namely lumazine synthase, proline racemase subunit A, lipoprotein outer membrane protein-19, and Cu-Zn superoxide dismutase. With an aim to develop a cross-protecting vaccine, Brucella pan-species conserved rBs were selected...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28614533/effect-of-fluoride-on-salivary-immunoglobulins-and-sialic-acid
#6
Kadriye Görkem Ulu Güzel, Zuhal Kirzioğlu, Ali Kudret Adiloğlu, Münciye Semra Özay Ertürk
Objective: The aim of our study was to evaluate the effect of fluoride on salivary immunoglobulin and sialic acid levels in children with dental fluorosis and healthy teeth who live in places with high fluoride concentration in drinking water. Method: Fifty-one (51) healthy children between 6 and 12 years old with no caries were randomly selected from primary schools enrolled in the dental-care program operated by the Department of Pediatric Dentistry. The children were divided into two groups: group I comprised 26 children with dental fluorosis [Thylstrup-Fejerskov Dental Fluorosis Index (TFI) = 4] who lived in Isparta (2...
April 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28611957/genetic-virulence-profile-of-enteroaggregative-escherichia-coli-strains-isolated-from-danish-children-with-either-acute-or-persistent-diarrhea
#7
Betina Hebbelstrup Jensen, Anja Poulsen, Stig Hebbelstrup Rye Rasmussen, Carsten Struve, Jørgen H Engberg, Alice Friis-Møller, Nadia Boisen, Rie Jønsson, Randi F Petersen, Andreas M Petersen, Karen A Krogfelt
Enteroaggregative Escherichia coli (EAEC) is frequently found in diarrheal stools worldwide. It has been associated with persistent diarrhea, weight loss, and failure to thrive in children living in developing countries. A number of important EAEC virulence genes are identified; however, their roles in acute and persistent diarrhea have not been previously investigated. The aim of this study was to identify specific EAEC virulence genes associated with duration and type of diarrhea in Danish children. We aimed to improve the current diagnostics of EAEC and enable targeting of strains with an expected severe disease course...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28599261/secretory-immunoglobulin-purification-from-whey-by-chromatographic-techniques
#8
Alexander Matlschweiger, Hannah Engelmaier, Gottfried Himmler, Rainer Hahn
Secretory immunoglobulins (SIg) are a major fraction of the mucosal immune system and represent potential drug candidates. So far, platform technologies for their purification do not exist. SIg from animal whey was used as a model to develop a simple, efficient and potentially generic chromatographic purification process. Several chromatographic stationary phases were tested. A combination of two anion-exchange steps resulted in the highest purity. The key step was the use of a small-porous anion exchanger operated in flow-through mode...
May 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28594235/live-attenuated-auxotrophic-mutant-of-salmonella-typhimurium-expressing-immunogenic-ha1-protein-enhances-immunity-and-protective-efficacy-against-h1n1-influenza-virus-infection
#9
Nitin Machindra Kamble, Kim Je Hyoung, John Hwa Lee
AIM: To evaluate the efficacy of attenuated Salmonella typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264...
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28593178/association-of-toll-like-cell-receptors-tlr2-p-arg753gln-and-tlr4-p-asp299gly-polymorphisms-with-indicators-of-general-and-local-immunity-in-patients-with-atopic-dermatitis
#10
Yury A Tyurin, Anton F Shamsutdinov, Nikolay N Kalinin, Alsou A Sharifullina, Irina D Reshetnikova
A whole group of polymorphisms of genes involved in the formation of the epidermal barrier, immune responses, and their regulation is important in the formation of atopic phenotype. The purpose of the study is to determine the relationship of polymorphisms of genes of Toll-like receptors TLR2 and TLR4 with clinical and immunological parameters in atopic dermatitis patients in a "case-control" study. Polymorphisms of genes TLR2 (p.Arg753Gln) and TLR4 (Asp299Gly) were detected by PCR. Parameters of the state of innate and adaptive immunity were assessed by the level of local production of sIgA, cytokine profile of blood serum for IL-4, IL-10, and IFN-γ...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28587925/siga-shigella-immune-complexes-interact-with-dectin-1-and-signr3-to-differentially-regulate-mouse-peyer-s-patch-and-mesenteric-lymph-node-dendritic-cell-s-responsiveness
#11
Josip Mikulic, Gilles Bioley, Blaise Corthésy
In addition to contributing to immune exclusion at mucosal surfaces, secretory IgA (SIgA) made of polymeric IgA and secretory component is able to selectively reenter via microfold cells into Peyer's patches (PPs) present along the intestine and to associate with dendritic cells (DCs) of the CD11c(+)CD11b(+)MHCII(+)F4/80(-)CD8(-)phenotype in the subepithelial dome region and the draining mesenteric lymph nodes (MLNs). However, the nature of the receptor(s) for SIgA on murine PP and MLN DCs is unknown. We find that glycosylated secretory component moiety and polymeric IgA are both involved in the specific interaction with these cells...
June 3, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28585763/a-combinatorial-strategy-of-alternative-promoter-use-during-differentiation-of-a-heterocystous-cyanobacteria
#12
Alicia M Muro-Pastor, Manuel Brenes-Álvarez, Agustín Vioque
Heterocystous cyanobacteria such as Nostoc sp. are filamentous photosynthetic organisms that, in response to nitrogen deficiency, undergo a differentiation process transforming certain, semi-regularly spaced cells into heterocysts, devoted to nitrogen fixation. During transition to a nitrogen-fixing regime, growth of most vegetative cells in the filament is temporarily arrested due to nutritional deprivation, but developing heterocysts require intense transcriptional activity. Therefore, the coexistence of arrested vegetative cells and actively developing prospective heterocysts relies on the simultaneous operation of somewhat opposite transcriptional programs...
June 6, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28577529/the-effect-of-immunoregulation-of-streptococcus-lactis-l16-strain-upon-staphylococcus-aureus-infection
#13
Maopeng Wang, Shengjie Gong, Shouwen Du, Yilong Zhu, Fengjun Rong, Rongrong Pan, Yang Di, Chang Li, Dayong Ren, Ningyi Jin
BACKGROUND: Staphylococcus aureus is an important pathogen that causes various infections in medical facilities. However, resistance to multiple drugs has made this infection difficult to manage. Thus, new therapeutic strategies are urgently needed to solve this worldwide public health problem. The Streptococcus lactis L16 strain was isolated from the fermented hot chili sauce. To explore whether it can be used as a protective agent against S. aureus infection, we designed a mouse model of S...
June 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28573797/is-salivary-iga-level-a-potential-biomarker-for-immunosuppression-in-hiv-positive-children-a-systematic-review-and-meta-analysis
#14
REVIEW
Fawad Javed, Zohaib Akram, Munerah Saleh Binshabaib, Shatha Subhi ALHarthi, Sergio Varela Kellesarian, Fahim Vohra
The aim of this systematic review was to determine whether or not assessment of salivary secretory immunoglobulin A (sIgA) levels could be a potential biomarker for immunosuppression in HIV-positive children. The Patient, Exposure, Comparative, Outcome question was "Is sIgA level a potential biomarker for immunosuppression in HIV-positive children?" Electronic and manual literature searches were conducted in indexed databases (MEDLINE, PubMed, EMBASE, ScienceDirect, and SCOPUS databases) up to and including June 2017...
June 1, 2017: Reviews in Medical Virology
https://www.readbyqxmd.com/read/28553275/host-immune-selection-of-rumen-bacteria-through-salivary-secretory-iga
#15
Janelle M Fouhse, Luke Smiegielski, Melanie Tuplin, Le Luo Guan, Benjamin P Willing
The rumen microbiome is integral to efficient production in cattle and shows strong host specificity, yet little is known about what host factors shape rumen microbial composition. Secretory immunoglobulin A (SIgA) is produced in large amounts in the saliva, can coat both commensal and pathogenic microbes within the gut, and presents a plausible mechanism of host specificity. However, the role salivary SIgA plays in commensal bacteria selection in ruminants remains elusive. The main objectives of this study were to develop an immuno-affinity benchtop method to isolate SIgA-tagged microbiota and to determine if salivary SIgA preferentially binds selected bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28527759/selection-of-new-lactic-acid-bacteria-strains-bearing-probiotic-features-from-mucosal-microbiota-of-healthy-calves-looking-for-immunobiotics-through-in-vitro-and-in-vivo-approaches-for-immunoprophylaxis-applications
#16
Sávio Sandes, Luige Alvim, Bruno Silva, Leonardo Acurcio, Cinara Santos, Márcia Campos, Camila Santos, Jacques Nicoli, Elisabeth Neumann, Álvaro Nunes
From the birth, since their mucosal microbiota and immune system are not fully developed, newborn calves are susceptible to several mucosal pathogenic microorganisms. Operating through humoral and non-humoral mechanisms in the host, several lactic acid bacteria strains bearing probiotic features are often employed in livestock as food supplement, improving animal production performance, promoting health and reducing the severity of mucosal infections. Accordingly, we isolated, species-level identified and screened for their probiotic potentials seventy lactic acid bacteria strains from upper airway, vaginal and intestinal mucosa of healthy calves...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28525303/low-level-laser-therapy-after-wisdom-teeth-surgery-evaluation-of-immunologic-markers-secretory-immunoglobulin-a-and-lysozyme-levels-and-thermographic-examination-placebo-controlled-study
#17
Tatjana Dostalova, Veronika Kroulikova, Stepan Podzimek, Helena Jelinková
OBJECTIVE: The goal of this investigation is to analyze the effect of therapeutic low-level laser therapy (LLLT) to have a possibility to check pain and inflammation connected with surgical removal of impacted lower third molars in general anesthesia or even phobia [not accompanied by pain or fear of dental treatment, using immunologic markers-secretory immunoglobulin A (sIgA) and lysozyme]. The healing process was also monitored by infrared thermography. BACKGROUND DATA: LLLT can accelerate the proliferation phase of healing and decrease the inflammatory reaction, but the effect is not really clear...
May 16, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28498440/gastrin-induces-multidrug-resistance-via-the-degradation-of-p27kip1-in-the-gastric-carcinoma-cell-line-sgc7901
#18
Kun Zhuang, Lingxia Zhang, Xin Zhang, Hailing Tang, Jun Zhang, Yuan Yan, Kun Han, Hanqing Guo
Multidrug resistance (MDR) is one of the major reasons for the failure of chemotherapy-based gastric carcinoma (GC) treatments, hence, biologically based therapies are urgently needed. Gastrin (GAS), a key gastrointestinal (GI) hormone, was found to be involved in tumor formation, progression, and metastasis. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining analysis revealed a high level of expression of GAS in drug-insensitive GC tissues (P<0.01) and similar results were revealed in GC cell lines SGC7901 and its multidrug-resistant variants SGC7901/VCR and SGC7901/ADR...
June 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28496443/higher-levels-of-secretory-iga-are-associated-with-low-disease-activity-index-in-patients-with-reactive-arthritis-and-undifferentiated-spondyloarthritis
#19
Fabián Salas-Cuestas, Wilson Bautista-Molano, Juan M Bello-Gualtero, Ivonne Arias, Diana Marcela Castillo, Lorena Chila-Moreno, Rafael Valle-Oñate, Daniel Herrera, Consuelo Romero-Sánchez
INTRODUCTION: Both reactive arthritis (ReA) and undifferentiated spondyloarthritis (uSpA) belong to the group of autoinflammatory diseases called spondyloarthritis (SpA). Hypotheses have been proposed about a relationship between the intestinal mucosa and inflammation of joint tissues. The role of immunoglobulin IgA or secretory immunoglobulin A (SIgA) in the inflammatory and/or clinical activity of patients with SpA remains poorly understood. OBJECTIVE: To evaluate the status of total IgA and SIgA, and the association among the levels of SIgA, IgA, IgA anti-Chlamydia trachomatis, and anti-Shigella spp...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28487998/effects-of-dietary-l-tryptophan-supplementation-on-intestinal-response-to-chronic-unpredictable-stress-in-broilers
#20
Yunshuang Yue, Yuming Guo, Ying Yang
Stress has been recognized as a critical risk factor for gastrointestinal diseases in both humans and animals. However, nutritional strategies to attenuate stress-induced intestinal barrier function and underlying mechanisms remain largely unknown. This study tested the hypothesis that L-tryptophan enhanced intestinal barrier function by regulating mucosal serotonin metabolism in chronic unpredictable stress-exposed broilers. One-day-old male broilers (Arbor Acres) were fed a basal diet supplemented with or without L-tryptophan in the absence or presence of chronic unpredictable stress...
May 9, 2017: Amino Acids
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