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https://www.readbyqxmd.com/read/28819629/mixed-lactobacillus-plantarum-strains-inhibit-staphylococcus-aureus-induced-inflammation-and-ameliorate-intestinal-microflora-in-mice
#1
Dayong Ren, Shengjie Gong, Jingyan Shu, Jianwei Zhu, Fengjun Rong, Zhenye Zhang, Di Wang, Liangfeng Gao, Tianming Qu, Hongyan Liu, Ping Chen
Objective. Staphylococcus aureus is an important pathogen that causes intestinal infection. We examined the immunomodulatory function of single and mixed Lactobacillus plantarum strains, as well as their impacts on the structure of the microbiome in mice infected with Staphylococcus aureus. The experiment was divided into three groups: protection, treatment, and control. Serum IFN-γ and IL-4 levels, as well as intestinal sIgA levels, were measured during and 1 week after infection with Staphylococcus aureus with and without Lactobacillus plantarum treatment...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28811748/salivary-diagnostic-markers-in-males-and-females-during-rest-and-exercise
#2
Kay Rutherfurd-Markwick, Carlene Starck, Deborah K Dulson, Ajmol Ali
BACKGROUND: Saliva is a useful diagnostic tool for analysis in sports, exercise and nutrition research, as collection is easy and non-invasive and it contains a large number of analytes affected by a range of physiological and pathological stressors and conditions. This study examined key salivary electrolytes and stress and immune markers in males and females at rest and during exercise. METHODS: Unstimulated whole saliva from 20 healthy, recreationally active participants (8 males and 12 females) was analysed for flow rate, osmolality, sodium (Na(+)), potassium (K(+)), chloride (Cl(-)), secretory immunoglobulin A (SIgA), α-amylase activity and cortisol during both rest and moderate intensity (70% peak power) cycling exercise in a randomised crossover design...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28811215/molecular-mechanisms-underlying-protection-against-h9n2-influenza-virus-challenge-in-mice-by-recombinant-lactobacillus-plantarum-with-surface-displayed-ha2-ltb
#3
Yanlong Jiang, Guilian Yang, Qi Wang, Zhannan Wang, Wentao Yang, Wei Gu, Chunwei Shi, Jianzhong Wang, Haibin Huang, Chunfeng Wang
It has been considered that the Avian influenza virus (AIV) causes severe threats to poultry industry. In this study, we constructed a series of recombinant Lactobacillus plantarum (L. plantarum) with surface displayed hemagglutinin subunit 2 (HA2) alone or together with heat-labile toxin B subunit (LTB) from enterotoxigenic Escherichia coli. Balb/c mice were used as model to evaluate the protective effects of recombinant L. plantarum strains against H9N2 subtype challenge. The results showed that the presence of LTB significantly increased the percentages of CD3(+)CD4(+)IL-4(+), CD3(+)CD4(+)IFN-γ(+) and CD3(+)CD4(+)IL-17(+) T cells, as well as CD3(+)CD8(+)IFN-γ(+) T cells in spleen and MLNs determined by Fluorescence-Activated Cell Sorting assay...
August 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28792376/construction-of-an-inactivated-typhoid-vaccine-candidate-expressing-escherichia-coli-heat-labile-enterotoxin-b-subunit-and-evaluation-of-its-immunogenicity-in-a-murine-model
#4
Boram Kim, Gayeon Won, John Hwa Lee
PURPOSE: Typhoid fever caused by Salmonella enterica serovar Typhi has contributed to the global public health burden, particularly in developing countries. In this study, an S. Typhi ghost was developed and its capacity as a vaccine candidate against typhoid fever was assessed. METHODOLOGY: An asd+ plasmid pJHL187 harbouring a ghost cassette comprising the PhiX 174 Elysis gene tightly controlled under the convergent promotor system was transformed into an asd gene-deleted mutant S...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28790976/factors-affecting-measurement-of-salivary-cortisol-and-secretory-immunoglobulin-a-in-field-studies-of-athletes
#5
Barry Thomas Pritchard, Warren Stanton, Roger Lord, Peter Petocz, Gert-Jan Pepping
AIMS: Biological and lifestyle factors, such as daily rhythm, caffeine ingestion, recent infection, and antibiotic intake, have been shown to influence measurements of salivary cortisol (SC) and secretory immunoglobulin A (sIgA). Current methodology in unsynchronized, field-based biomarker studies does not take these effects into account. Moreover, very little is known about the combined effects of biological and lifestyle factors on SC and sIgA. This study supports development of a protocol for measuring biomarkers from saliva collected in field studies by examining the individual and combined effects of these factors on SC and sIgA...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28752945/synthesis-of-the-compatible-solute-proline-by-bacillus-subtilis-point-mutations-rendering-the-osmotically-controlled-prohj-promoter-hyperactive
#6
Tamara Hoffmann, Monika Bleisteiner, Praveen Kumar Sappa, Leif Steil, Ulrike Mäder, Uwe Völker, Erhard Bremer
The ProJ and ProH enzymes of Bacillus subtilis catalyze together with ProA (ProJ-ProA-ProH), osmostress-adaptive synthesis of the compatible solute proline. The proA-encoded gamma-glutamyl phosphate reductase is also used for anabolic proline synthesis (ProB-ProA-ProI). Transcription of the proHJ operon is osmotically inducible whereas that of the proBA operon is not. Targeted and quantitative proteome analysis revealed that the amount of ProA is not limiting for the interconnected anabolic and osmostress-responsive proline production routes...
July 28, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28744263/recombinant-invasive-lactococcus-lactis-carrying-a-dna-vaccine-coding-the-ag85a-antigen-increases-inf-%C3%AE-il-6-and-tnf-%C3%AE-cytokines-after-intranasal-immunization
#7
Pamela Mancha-Agresti, Camila Prosperi de Castro, Janete S C Dos Santos, Maíra A Araujo, Vanessa B Pereira, Jean G LeBlanc, Sophie Y Leclercq, Vasco Azevedo
Tuberculosis (TB) remains a major threat throughout the world and in 2015 it caused the death of 1.4 million people. The Bacillus Calmette-Guérin is the only existing vaccine against this ancient disease; however, it does not provide complete protection in adults. New vaccines against TB are eminently a global priority. The use of bacteria as vehicles for delivery of vaccine plasmids is a promising vaccination strategy. In this study, we evaluated the use of, an engineered invasive Lactococcus lactis (expressing Fibronectin-Binding Protein A from Staphylococcus aureus) for the delivery of DNA plasmid to host cells, especially to the mucosal site as a new DNA vaccine against tuberculosis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28744226/the-mucosal-immune-function-is-not-compromised-during-a-period-of-high-intensity-interval-training-is-it-time-to-reconsider-an-old-assumption
#8
Dennis-Peter Born, Christoph Zinner, Billy Sperlich
Purpose: The aim of the study was to evaluate the mucosal immune function and circadian variation of salivary cortisol, Immunoglobin-A (sIgA) secretion rate and mood during a period of high-intensity interval training (HIIT) compared to long-slow distance training (LSD). Methods: Recreational male runners (n = 28) completed nine sessions of either HIIT or LSD within 3 weeks. The HIIT involved 4 × 4 min of running at 90-95% of maximum heart rate interspersed with 3 min of active recovery while the LSD comprised of continuous running at 70-75% of maximum heart rate for 60-80 min...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28709802/human-secretory-igm-emerges-from-plasma-cells-clonally-related-to-gut-memory-b-cells-and-targets-highly-diverse-commensals
#9
Giuliana Magri, Laura Comerma, Marc Pybus, Jordi Sintes, David Lligé, Daniel Segura-Garzón, Sabrina Bascones, Ada Yeste, Emilie K Grasset, Cindy Gutzeit, Mathieu Uzzan, Meera Ramanujam, Menno C van Zelm, Raquel Albero-González, Ivonne Vazquez, Mar Iglesias, Sergi Serrano, Lucía Márquez, Elena Mercade, Saurabh Mehandru, Andrea Cerutti
Secretory immunoglobulin A (SIgA) enhances host-microbiota symbiosis, whereas SIgM remains poorly understood. We found that gut IgM(+) plasma cells (PCs) were more abundant in humans than mice and clonally related to a large repertoire of memory IgM(+) B cells disseminated throughout the intestine but rare in systemic lymphoid organs. In addition to sharing a gut-specific gene signature with memory IgA(+) B cells, memory IgM(+) B cells were related to some IgA(+) clonotypes and switched to IgA in response to T cell-independent or T cell-dependent signals...
July 18, 2017: Immunity
https://www.readbyqxmd.com/read/28700735/molecular-mechanisms-by-which-casein-glycomacropeptide-maintains-internal-homeostasis-in-mice-with-experimental-ulcerative-colitis
#10
Yongbo Cui, Chenchen Zhu, Zhu Ming, Jiangming Cao, Yali Yan, Pei Zhao, Guangchang Pang, Zixin Deng, Yi Yao, Qingsen Chen
OBJECTIVES: The aim of this study was to elucidate the molecular mechanisms by which food-derived casein glycomacropeptide (CGMP) maintains internal homeostasis in the intestinal mucosa and to investigate the effects of CGMP on the intestinal mucosal immunological barrier and related signal transduction pathways. METHODS: In this study, a famoxadone (OXZ)-induced mouse experimental ulcerative colitis (UC) model was built. The experimental UC mice were intragastrically administered milk-derived CGMP for four consecutive days...
2017: PloS One
https://www.readbyqxmd.com/read/28685588/recent-advances-on-biodegradable-polymeric-carrier-based-mucosal-immunization-an-overview
#11
Lovedeep Kaur, Ankush Sharma, Awesh Kumar Yadav, Neeraj Mishra
Mucosal administration of vaccine is most prevalent way to induce desired immunity against various types of antigen and microbial in central and in addition, the peripheral blood in most external mucosal surface. Mucosal delivery of vaccine provides both humoral and cellular responses against mucosal infection. Mucosa, which are exposed to heavy loads of commensal and pathogenic microorganism, are one of the main region where infections are built up, also, thus have frontline status in immunity, making mucosa perfect site for vaccines application...
July 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28662707/oral-symptoms-and-salivary-findings-in-oral-lichen-planus-oral-lichenoid-lesions-and-stomatitis
#12
Kristine Roen Larsen, Jeanne Duus Johansen, Jesper Reibel, Claus Zachariae, Kasper Rosing, Anne Marie Lynge Pedersen
BACKGROUND: To examine if patients with oral lichen planus, oral lichenoid lesions and generalised stomatitis and concomitant contact allergy have more frequent and severe xerostomia, lower unstimulated and chewing-stimulated saliva and citric-acid-stimulated parotid saliva flow rates, and higher salivary concentration of total protein and sIgA than cases without contact allergy and healthy controls. METHODS: Forty-nine patients (42 women, aged 61.0 ± 10.3 years) and 29 healthy age- and gender-matched subjects underwent a standardised questionnaire on general and oral health, assessment of xerostomia, clinical examination, sialometry, mucosal biopsy and contact allergy testing...
June 29, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28653779/adrenal-maturation-nutritional-status-and-mucosal-immunity-in-bolivian-youth
#13
Carolyn R Hodges-Simeon, Sean P Prall, Aaron D Blackwell, Michael Gurven, Steven J C Gaulin
OBJECTIVES: Humans-and several other apes-exhibit a unique pattern of post-natal adrenal maturation; however, the causes and consequences of variation in adrenal development are not well understood. In this study, we examine developmental and age-related maturation of the adrenal gland (measured via dehydroepiandrosterone-sulfate [DHEA-S]) for potential life-history associations with growth and mucosal immunity in a rural population of immune-challenged Bolivian juveniles and adolescents...
June 27, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/28651382/assignment-of-sigma-factors-of-rna-polymerase-to-promoters-in-corynebacterium-glutamicum
#14
Hana Dostálová, Jiří Holátko, Tobias Busche, Lenka Rucká, Andrey Rapoport, Petr Halada, Jan Nešvera, Jörn Kalinowski, Miroslav Pátek
Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σ(A)), the primary-like σ(B) and five alternative sigma factors (σ(C), σ(D), σ(E), σ(H) and σ(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28648455/kinetic-modelling-and-meta-analysis-of-the-b-subtilis-siga-regulatory-network-during-spore-germination-and-outgrowth
#15
O Ramaniuk, M Černý, L Krásný, J Vohradský
This study describes the meta-analysis and kinetic modelling of gene expression control by sigma factor SigA of Bacillus subtilis during germination and outgrowth based on microarray data from 14 time points. The analysis computationally models the direct interaction among SigA, SigA-controlled sigma factor genes (sigM, sigH, sigD, sigX), and their target genes. Of the >800 known genes in the SigA regulon, as extracted from databases, 311 genes were analysed, and 190 were confirmed by the kinetic model as being controlled by SigA...
June 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28645157/secretory-iga-response-against-pseudomonas-aeruginosa-in-the-upper-airways-and-the-link-with-chronic-lung-infection-in-cystic-fibrosis
#16
Renan Mauch, Claudio Rossi, Talita Aiello, José Ribeiro, Antonio Ribeiro, Niels Høiby, Carlos Levy
We assessed the diagnostic ability of an ELISA test for measurement of specific secretory IgA (sIgA) in saliva to identify Cystic Fibrosis (CF) patients with P. aeruginosa chronic lung infection and intermittent lung colonization. A total of 102 Brazilian CF patients and 53 healthy controls were included. Specific serum IgG response was used as a surrogate to distinguish CF patients according to their P. aeruginosa colonization/infection status. The rate of sIgA positivity was 87.1% in CF chronically infected patients (Median value = 181...
June 22, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28633646/role-of-serotonin-on-the-intestinal-mucosal-immune-response-to-stress-induced-diarrhea-in-weaning-mice
#17
Yulan Dong, Yanan Han, Zixu Wang, Zhuoming Qin, Chenyu Yang, Jing Cao, Yaoxing Chen
BACKGROUND: During weaning, babies and young animal often experience diarrhea from food intolerance and/or decreasing levels of maternal antibodies, and diarrhea tends to be particularly severe during the early-weaned period, which often exhibits an underdeveloped immune system, a disturbed gut environment and results in nutrient malabsorption and dehydration. It was deduced that neuroendocrine might have close relation with diarrhea, especially 5-HT. METHODS: To explore the role of serotonin (5-HT) in weaning mice subjected to stress-induced diarrhea, 21-day-old weaned mice were divided into the following groups: control group, stress-induced diarrhea group (restrained by binding the hind limbs and intragastric administration of folium sennae with 0...
June 21, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28630108/inducing-mucosal-iga-a-challenge-for-vaccine-adjuvants-and-delivery-systems
#18
REVIEW
Prosper N Boyaka
Mucosal IgA or secretory IgA (SIgA) are structurally equipped to resist chemical degradation in the harsh environment of mucosal surfaces and enzymes of host or microbial origin. Production of SIgA is finely regulated, and distinct T-independent and T-dependent mechanisms orchestrate Ig α class switching and SIgA responses against commensal and pathogenic microbes. Most infectious pathogens enter the host via mucosal surfaces. To provide a first line of protection at these entry ports, vaccines are being developed to induce pathogen-specific SIgA in addition to systemic immunity achieved by injected vaccines...
July 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28625525/recombinant-lactobacillus-casei-expressing-clostridium-perfringens-toxoids-%C3%AE-%C3%AE-2-%C3%AE%C2%B5-and-%C3%AE-1-gives-protection-against-clostridium-perfringens-in-rabbits
#19
Li Zhao, Zhihou Guo, Jiali Liu, Zi Wang, Ruichong Wang, Yijing Li, Li Wang, Yigang Xu, Lijie Tang, Xinyuan Qiao
The present study used Lactobacillus casei ATCC 393 as antigen delivery system to express C. perfringens toxoids α-β2-ε-β1 to construct the recombination Lactobacillus casei pPG-2-α-β2-ε-β1/L. casei 393. After being induced by 1% xylose, the specificity and integrity of recombinant strain were determined by Western-blotting. Rabbits as native animal model were immunized orally with pPG-2-α-β2-ε-β1/L. casei 393 and the titers of specific IgG and sIgA were determined by ELISA. The result showed that oral administration with the recombinants could elicit both local mucosal and systemic immune responses...
July 13, 2017: Vaccine
https://www.readbyqxmd.com/read/28622869/brucella-lipopolysaccharide-reinforced-salmonella-delivering-brucella-immunogens-protects-mice-against-virulent-challenge
#20
Jonathan Lalsiamthara, John Hwa Lee
Intracellular pathogen Salmonella exhibits natural infection broadly analogous to Brucella, this phenomenon makes Salmonella a pragmatic choice for an anti-Brucella vaccine delivery platform. In this study we developed and formulated a combination of four attenuated Salmonella Typhimurium live vector strains delivering heterologous Brucella antigens (rBs), namely lumazine synthase, proline racemase subunit A, lipoprotein outer membrane protein-19, and Cu-Zn superoxide dismutase. With an aim to develop a cross-protecting vaccine, Brucella pan-species conserved rBs were selected...
June 2017: Veterinary Microbiology
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