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https://www.readbyqxmd.com/read/27913413/new-arsenate-reductase-gene-arra-pcr-primers-for-diversity-assessment-and-quantification-in-environmental-samples
#1
Babur S Mirza, Darwin L Sorensen, R Ryan Dupont, Joan E McLean
: The extent of arsenic contamination in drinking water and its potential threat to human health have resulted in considerable research interest in the microbial species responsible for arsenic reduction. The arsenate reductase gene (arrA), an important component of the microbial arsenate reduction system, has been widely used as a biomarker to study arsenate reducing microorganisms. A new primer pair was designed and evaluated for qPCR and high-throughput sequencing of the arrA gene, because currently available PCR primers are not suitable for these applications...
December 2, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27912981/examining-the-association-between-surface-bioburden-and-frequently-touched-sites-in-intensive-care
#2
C E Adams, J Smith, V Watson, C Robertson, S J Dancer
BACKGROUND: Critical care patients are at increased risk of infection. Near-patient surfaces act as reservoirs of microbial soil, which may contain pathogens. AIM: To correlate soil levels with hand-touch frequency of near-patient sites in an intensive care unit (ICU). METHODS: Five sites around each bed in a 10-bed ICU were screened for total microbial soil (cfu/cm(2)) and Staphylococcus aureus every month for 10 months. Selected sites were infusion pump and cardiac monitor, left and right bedrails, and bed table...
November 13, 2016: Journal of Hospital Infection
https://www.readbyqxmd.com/read/27912185/sorption-of-vanadium-v-onto-natural-soil-colloids-under-various-solution-ph-and-ionic-strength-conditions
#3
Xiuhua Luo, Lin Yu, Changzhao Wang, Xianqiang Yin, Ahmed Mosa, Jialong Lv, Huimin Sun
Batch sorption kinetics and isothermal characteristics of V(V) were investigated on three natural soil colloids (manual loessial soil colloid (MSC), aeolian sandy soil colloid (ASC), and cultivated loessial soil colloid (CSC)) under various solution pH and ionic strength (IS) conditions. Colloids were characterized by atomic force microscopy (AFM), X-ray diffraction (XRD), and fourier transform infrared spectroscopy (FTIR). AFM micrographs showed CSC with an aggregated shape with larger particle diameter as compared with ASC and MSC...
November 29, 2016: Chemosphere
https://www.readbyqxmd.com/read/27911419/methods-of-soil-resampling-to-monitor-changes-in-the-chemical-concentrations-of-forest-soils
#4
Gregory B Lawrence, Ivan J Fernandez, Paul W Hazlett, Scott W Bailey, Donald S Ross, Thomas R Villars, Angelica Quintana, Rock Ouimet, Michael R McHale, Chris E Johnson, Russell D Briggs, Robert A Colter, Jason Siemion, Olivia L Bartlett, Olga Vargas, Michael R Antidormi, Mary M Koppers
Recent soils research has shown that important chemical soil characteristics can change in less than a decade, often the result of broad environmental changes. Repeated sampling to monitor these changes in forest soils is a relatively new practice that is not well documented in the literature and has only recently been broadly embraced by the scientific community. The objective of this protocol is therefore to synthesize the latest information on methods of soil resampling in a format that can be used to design and implement a soil monitoring program...
November 25, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27908626/intercropped-pteris-vittata-l-and-morus-alba-l-presents-a-safe-utilization-mode-for-arsenic-contaminated-soil
#5
Xiaoming Wan, Mei Lei, Tongbin Chen, Junxing Yang
Intercropping technology provides income for owners of contaminated soil without increasing environmental risk. Therefore, intercropping of arsenic (As) hyperaccumulator Pteris vittata L. with economic crops is now widely utilized in slightly or moderately As-contaminated farmlands. However, the mechanisms for As mobilization and absorption within the intercropping system are still unclear. To clarify As mobilization and absorption within an intercropping system, portable X-ray fluorescence spectrometry and sequential extraction were utilized to detect the spatial distribution and speciation of As in an intercropped system of P...
November 28, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27908623/effects-of-growing-seasons-and-genotypes-on-the-accumulation-of-cadmium-and-mineral-nutrients-in-rice-grown-in-cadmium-contaminated-soil
#6
Yang Liu, Changbo Zhang, Yanling Zhao, Shoujun Sun, Zhongqi Liu
Heavy metals naturally occur in soil but their concentrations may be changed by seasonal rainfall under double-rice cropping system. The field trials at three sites, which represent low, medium and high cadmium (Cd) content in soil, revealed significant genotypic and environmental variations in grain Cd concentrations. Most cultivars in late rice at three sites produced grains with Cd content over the maximum permissible concentration (MPC) 0.20mgkg(-1). However, grain Cd content in early rice was over MPC only at high Cd site...
November 28, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27908488/accumulation-of-cadmium-and-uranium-in-arable-soils-in-switzerland
#7
Moritz Bigalke, Andrea Ulrich, Agnes Rehmus, Armin Keller
Mineral phosphorus (P) fertilizers contain contaminants that are potentially hazardous to humans and the environment. Frequent mineral P fertilizer applications can cause heavy metals to accumulate and reach undesirable concentrations in agricultural soils. There is particular concern about Cadmium (Cd) and Uranium (U) accumulation because these metals are toxic and can endanger soil fertility, leach into groundwater, and be taken up by crops. We determined total Cd and U concentrations in more than 400 topsoil and subsoil samples obtained from 216 agricultural sites across Switzerland...
November 28, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27908415/iodine-in-the-feed-of-cows-and-in-the-milk-with-a-view-to-the-consumer-s-iodine-supply
#8
Friedrich Schöne, Kathrin Spörl, Matthias Leiterer
Milk may be an iodine source for humans, but its magnitude depends on the iodine content of cow feed. The present investigation focused on the iodine level of feed and milk in German feeding practice in comparison with the results of previous milk monitoring and dose-response experiments with dairy cows. In 73 samples of straight feedstuffs (41 concentrates, 32 silages prepared from grass or green maize) and a total of 83 vitamin mineral premixes and compound feeds, respectively, the iodine content was determined and along with the expected daily iodine intake of cows...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27905935/development-of-a-filter-to-prevent-infections-with-spore-forming-bacteria-in-injecting-drug-users
#9
Nour Alhusein, Jenny Scott, Barbara Kasprzyk-Hordern, Albert Bolhuis
BACKGROUND: In heroin injectors, there have been a number of outbreaks caused by spore-forming bacteria, causing serious infections such as anthrax or botulism. These are, most likely, caused by injecting contaminated heroin, and our aim was to develop a filter that efficiently removes these bacteria and is also likely to be acceptable for use by people who inject drugs (i.e. quick, simple and not spoil the hit). METHODS: A prototype filter was designed and different filter membranes were tested to assess the volume of liquid retained, filtration time and efficiency of the filter at removing bacterial spores...
December 1, 2016: Harm Reduction Journal
https://www.readbyqxmd.com/read/27905820/evidence-based-validation-of-quorum-quenching-from-lysinibacillus-sphaericus-and-geobacillus-sp-in-bioremediation-of-oil-sludge
#10
Camilo Gómez-Garzón, Jenny Dussán
Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consortia in applications such as bioremediation. In the current work, we demonstrated the presence of a potential novel AHL-lactonase-encoding locus (Bsph_3377) from Lysinibacillus sphaericus and Geobacillus sp...
September 1, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27905626/-study-of-toxocara-sp-eggs-in-soils-of-public-areas-of-the-city-of-chill%C3%A3-n-chile
#11
Millaray Melín-Coloma, Carmen Villaguala-Pacheco, Raúl Lisboa-Navarro, Carlos Landaeta-Aqueveque
INTRODUCTION: Squares and public areas are mentioned frequently as source of infections by Toxocara sp. for people. There is no study of the presence of Toxocara sp. eggs in soils of the city of Chillán, Chile. AIMS: To assess the presence of toxocara eggs in the soils of public areas of Chillán. MATERIAL AND METHODS: 43 public areas were studied in January of 2014. Samples were processed by sedimentation-flotation method. In addition, two positive control assays were performed: one with feces contaminated with Toxocara eggs mixed with soil; and the other leaving the feces on the soil and then analyzing this soil...
August 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/27902938/functional-components-of-the-bacterial-czccba-efflux-system-reduce-cadmium-uptake-and-accumulation-in-transgenic-tobacco-plants
#12
Andrea Nesler, Giovanni DalCorso, Elisa Fasani, Anna Manara, Gian Pietro Di Sansebastiano, Emanuele Argese, Antonella Furini
Cadmium (Cd) is a toxic trace element released into the environment by industrial and agricultural practices, threatening the health of plants and contaminating the food/feed chain. Biotechnology can be used to develop plant varieties with a higher capacity for Cd accumulation (for use in phytoremediation programs) or a lower capacity for Cd accumulation (to reduce Cd levels in food and feed). Here we generated transgenic tobacco plants expressing components of the Pseudomonas putida CzcCBA efflux system. Plants were transformed with combinations of the CzcC, CzcB and CzcA genes, and the impact on Cd mobilization was analysed...
November 27, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27902329/abundance-of-antibiotic-resistance-genes-in-environmental-bacteriophages
#13
Taruna Anand, Bidhan Ch Bera, Rajesh K Vaid, Sanjay Barua, Thachamvally Riyesh, Nitin Virmani, Mubarik Hussain, Raj K Singh, Bhupendra N Tripathi
The ecosystem is continuously exposed to a wide variety of antimicrobials through waste effluents, agricultural runoffs, animal related and anthropogenic activities, which contribute to spread of antibiotic resistance genes (ARGs). The contamination of ecosystems with ARGs may create increased opportunities for their transfer to naive microbes and eventually lead to entry into the human food chain. Transduction is a significant mechanism of horizontal gene transfer in natural environments, which has traditionally been underestimated as compared to transformation...
October 21, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27902299/chryseobacterium-nepalense-sp-nov-isolated-from-oil-contaminated-soil
#14
Dhiraj Kumar Chaudhary, Jaisoo Kim
During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Biratnagar, Morang, Nepal, a yellow-coloured, Gram-staining negative, aerobic, non-motile, and rod shaped bacterium, designated strain C-5-3T, was isolated. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain C-5-3T belongs to the genus Chryseobacterium and is closely related to 'Chryseobacterium profundimaris' DY46 (98.19% sequence similarity), Chryseobacterium takakiae AG1-2T (98...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902243/rugosibacter-aromaticivorans-gen-nov-sp-nov-a-novel-bacterium-within-the-family-rhodocyclaceae-isolated-from-contaminated-soil-capable-of-degrading-aromatic-compounds
#15
Elizabeth M Corteselli, Michael D Aitken, David R Singleton
A bacterial strain designated Ca6T was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soil from the site of a former manufactured gas plant in Charlotte, North Carolina, USA and phylogenetically linked to the Rhodocyclaceae family of the Betaproteobacteria. Its 16S rRNA gene was highly similar to globally distributed environmental sequences, including those previously designated "Pyrene Group 1" demonstrated to grow on the PAHs phenanthrene and pyrene by stable-isotope probing. The closest described relative was Sulfuritalea hydrogenivorans strain sk43HT (93...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27898799/effects-of-dolomitic-limestone-application-on-zinc-speciation-in-boreal-forest-smelter-contaminated-soils
#16
Jordan G Hamilton, Richard E Farrell, Ning Chen, Joel Reid, Renfei Feng, Derek Peak
Anthropogenic activities at the HudBay Minerals, Inc., Flin Flon (Manitoba, Canada) mining and processing facility have severely affected the surrounding boreal forest ecosystem. Soil contamination occurred via a combination of metal and sulfuric acid deposition and has resulted in forest dieback and ineffective natural recovery. A community-led effort to revegetate areas of the landscape through the application of a dolomitic limestone has been met with varied success. Zinc (Zn) speciation has shown to be closely linked to the presence or absence of an invasive metal-tolerant grass species, with soils being broadly classed into two revegetation response groups...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898798/visualization-of-colloid-transport-pathways-in-mineral-soils-using-titanium-iv-oxide-as-a-tracer
#17
Stefan Koch, Petra Kahle, Bernd Lennartz
In soils, colloidal transport has been identified as the most important pathway for strong adsorbing, environmental contaminants like pesticides, heavy metals, and phosphorus. We conducted a comparative dye tracer experiment using a Brilliant Blue (BB) solution and a Titanium(IV) oxide (TiO) colloid suspension (average particle size 0.3 μm), aiming to visualize and quantify colloid pathways in soils. Both dye tracers showed comparable general flow patterns with preferred transport over the deepest part of the soil profile, independent of clay content...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898796/sorption-of-pharmaceuticals-heavy-metals-and-herbicides-to-biochar-in-the-presence-of-biosolids
#18
Daniel A Bair, Fungai N D Mukome, Inna E Popova, Temitope A Ogunyoku, Allie Jefferson, Daoyuan Wang, Sarah C Hafner, Thomas M Young, Sanjai J Parikh
Agricultural practices are increasingly incorporating recycled waste materials, such as biosolids, to provide plant nutrients and enhance soil functions. Although biosolids provide benefits to soil, municipal wastewater treatment plants receive pharmaceuticals and heavy metals that can accumulate in biosolids, and land application of biosolids can transfer these contaminants to the soil. Environmental exposure of these contaminants may adversely affect wildlife, disrupt microbial communities, detrimentally affect human health through long-term exposure, and cause the proliferation of antibiotic-resistant bacteria...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898791/high-removal-of-effluent-borne-nitrogen-with-multiple-external-electron-donors-in-the-engineered-drainfield-of-an-advanced-septic-system
#19
Mriganka De, Gurpal S Toor
Septic systems can be a major source of nitrogen (N) in shallow groundwater. We designed an in situ engineered drainfield with aerobic-anaerobic (sand-woodchips) and anaerobic (elemental sulfur-oyster shell) media to remove N in the vadose zone and reduce N transport to groundwater. Effluent was dispersed on top of the engineered drainfield (3.72 m infiltrative surface) and then infiltrated through the aerobic-anaerobic and anaerobic media before reaching natural soil. Water samples were collected over 64 sampling events (May 2012-December 2013) from three parts of the drainfield: (i) a suction cup lysimeter installed at the sand-woodchips interface, (ii) a pipe after effluent passed through the aerobic-anaerobic media, and (iii) a tank containing anaerobic media...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898779/production-of-biomass-crops-using-biowastes-on-low-fertility-soil-1-influence-of-biowastes-on-plant-and-soil-quality
#20
J Esperschuetz, C Anderson, S Bulman, O Lense, J Horswell, N Dickinson, R Hofmann, B H Robinson
Land application of biosolids to low-fertility soil can improve soil quality by increasing concentrations of macronutrients and trace elements. Mixing biosolids with sawdust could reduce the risks of contaminant accumulation posed by rebuilding soils using biosolids alone. We aimed to determine the effects of biosolids and biosolids-sawdust on the plant quality and chemical composition of sorghum, rapeseed, and ryegrass. Plants were grown in a greenhouse over a 5-mo period in a low-fertility soil amended with biosolids (1250 kg N ha), biosolids-sawdust (0...
November 2016: Journal of Environmental Quality
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