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https://www.readbyqxmd.com/read/27749902/high-throughput-analysis-of-global-dna-methylation-using-methyl-sensitive-digestion
#1
Hiromi Shiratori, Carmen Feinweber, Claudia Knothe, Jörn Lötsch, Dominique Thomas, Gerd Geisslinger, Michael J Parnham, Eduard Resch
DNA methylation is a major regulatory process of gene transcription, and aberrant DNA methylation is associated with various diseases including cancer. Many compounds have been reported to modify DNA methylation states. Despite increasing interest in the clinical application of drugs with epigenetic effects, and the use of diagnostic markers for genome-wide hypomethylation in cancer, large-scale screening systems to measure the effects of drugs on DNA methylation are limited. In this study, we improved the previously established fluorescence polarization-based global DNA methylation assay so that it is more suitable for application to human genomic DNA...
2016: PloS One
https://www.readbyqxmd.com/read/27582016/understanding-process-in-group-based-intervention-delivery-social-network-analysis-and-intra-entity-variability-methods-as-windows-into-the-black-box
#2
Lauren Molloy Elreda, J Douglas Coatsworth, Scott D Gest, Nilam Ram, Katharine Bamberger
Although the majority of evidence-based programs are designed for group delivery, group process and its role in participant outcomes have received little empirical attention. Data were collected from 20 groups of participants (94 early adolescents, 120 parents) enrolled in an efficacy trial of a mindfulness-based adaptation of the Strengthening Families Program (MSFP). Following each weekly session, participants reported on their relations to group members. Social network analysis and methods sensitive to intraindividual variability were integrated to examine weekly covariation between group process and participant progress, and to predict post-intervention outcomes from levels and changes in group process...
November 2016: Prevention Science: the Official Journal of the Society for Prevention Research
https://www.readbyqxmd.com/read/27568347/the-use-of-the-oesophageal-doppler-in-perioperative-medicine-new-opportunities-in-research-and-clinical-practice
#3
Bernardo Bollen Pinto, Glen Atlas, Bart F Geerts, Karim Bendjelid
The oesophageal Doppler (OD) is a minimally invasive haemodynamic monitor used in the surgical theatre and the ICU. Using the OD, goal-directed therapy (GDT) has been shown to reduce perioperative complications in high-risk surgical patients. However, most GDT protocols currently in use are limited to stroke volume optimisation. In the present manuscript, we examine the conceptual models behind new OD-based measurements. These would provide the clinician with a comprehensive view of haemodynamic pathophysiology; including pre-load, contractility, and afterload...
August 27, 2016: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/27422991/effect-of-peep-blood-volume-and-inspiratory-hold-maneuvers-on-venous-return
#4
David Berger, Per W Moller, Alberto Weber, Andreas Bloch, Stefan Bloechlinger, Matthias Haenggi, Soren Sondergaard, Stephan M Jakob, Sheldon Magder, Jukka Takala
According to Guyton's model of circulation, mean systemic filling pressure (MSFP), right atrial pressure (RAP), and resistance to venous return (RVR) determine venous return. MSFP has been estimated from inspiratory hold-induced changes in RAP and blood flow. We studied the effect of positive end-expiratory pressure (PEEP) and blood volume on venous return and MSFP in pigs. MSFP was measured by balloon occlusion of the right atrium (MSFPRAO), and the MSFP obtained via extrapolation of pressure-flow relationships with airway occlusion (MSFPinsp_hold) was extrapolated from RAP/pulmonary artery flow (QPA) relationships during inspiratory holds at PEEP 5 and 10 cmH2O, after bleeding, and in hypervolemia...
September 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27199311/the-effect-of-propofol-on-haemodynamics-cardiac-output-venous-return-mean-systemic-filling-pressure-and-vascular-resistances
#5
F de Wit, A L van Vliet, R B de Wilde, J R Jansen, J Vuyk, L P Aarts, E de Jonge, D P Veelo, B F Geerts
BACKGROUND: Although arterial hypotension occurs frequently with propofol use in humans, its effects on intravascular volume and vascular capacitance are uncertain. We hypothesized that propofol decreases vascular capacitance and therefore decreases stressed volume. METHODS: Cardiac output (CO) was measured using Modelflow(®) in 17 adult subjects after upper abdominal surgery. Mean systemic filling pressure (MSFP) and vascular resistances were calculated using venous return curves constructed by measuring steady-state arterial and venous pressures and CO during inspiratory hold manoeuvres at increasing plateau pressures...
June 2016: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/26206551/toward-isometric-force-capabilities-evaluation-by-using-a-musculoskeletal-model-comparison-with-direct-force-measurement
#6
Vincent Hernandez, Nasser Rezzoug, Philippe Gorce
Developing formalisms to determine force capabilities of human limbs by using musculoskeletal models could be useful for biomechanical and ergonomic applications. In this framework, the purpose of this study was to compare measured maximal isometric force capabilities at the hand in a set of Cartesian directions with forces computed from a musculoskeletal model of the upper-limb. The results were represented under the form of a measured force polytope (MFP) and a musculoskeletal force polytope (MSFP). Both of them were obtained from the convex hull of measured and simulated force vectors endpoints...
September 18, 2015: Journal of Biomechanics
https://www.readbyqxmd.com/read/25365122/integrating-mindfulness-with-parent-training-effects-of-the-mindfulness-enhanced-strengthening-families-program
#7
RANDOMIZED CONTROLLED TRIAL
J Douglas Coatsworth, Larissa G Duncan, Robert L Nix, Mark T Greenberg, Jochebed G Gayles, Katharine T Bamberger, Elaine Berrena, Mary Ann Demi
There is growing support for the efficacy of mindfulness training with parents as an intervention technique to improve parenting skills and reduce risk for youth problem behaviors. The evidence, however, has been limited to small scale studies, many with methodological shortcomings. This study sought to integrate mindfulness training with parents into the Strengthening Families Program: For Parents and Youth 10-14 (SFP 10-14), an empirically-validated family-based preventive intervention. It used a randomized-controlled comparative effectiveness study design (N = 432 families, 31% racial/ethnic minority) to test the efficacy of the Mindfulness-Enhanced Strengthening Families Program (MSFP), compared to standard SFP 10-14 and a minimal-treatment home study control condition...
January 2015: Developmental Psychology
https://www.readbyqxmd.com/read/24483965/bacteriological-quality-and-food-safety-in-a-brazilian-school-food-program
#8
Samara Nagla Chaves Trindade, Julia Silva Pinheiro, Héllen Gonçalves de Almeida, Keyla Carvalho Pereira, Paulo de Souza Costa Sobrinho
INTRODUCTION: Food safety is a critical issue in school food program. OBJECTIVE: This study was conducted to assess the bacteriological quality and food safety practices of a municipal school food program (MSFP) in Jequitinhonha Valley, Brazil. MATERIALS AND METHODS: A checklist based on good manufacturing practices (GMP) for food service was used to evaluate food safety practices. Samples from foods, food contact surfaces, the hands of food handlers, the water supply and the air were collected to assess bacteriological quality in establishments that comprise the MSFP...
2014: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/22805171/impact-of-mood-disturbance-sleep-disturbance-fatigue-and-pain-among-patients-receiving-cancer-therapy
#9
K K F Cheng, R M W Yeung
This paper describes the prevalence of mood disturbance, sleep disturbance, fatigue and pain (MSFP), either alone or in combination in patients receiving cancer therapy, and determines its impact and whether it is a predictor for functional status and the impairment of quality of life (QoL). This is a cross-sectional study using secondary data from a sample of 214 patients being treated by chemotherapy or radiotherapy. In all, 87%, 68%, 66% and 38% of the patients reported MSFP respectively. Co-occurrence of any three and all of the four symptoms, were reported separately at rates of 29% and 31%...
January 2013: European Journal of Cancer Care
https://www.readbyqxmd.com/read/22316684/treatment-of-complex-heavy-metal-wastewater-using-a-multi-staged-ferrite-process
#10
Yao-Jen Tu, Chien-Kuei Chang, Chen-Feng You, Shan-Li Wang
Complete removal of heavy metal from complex heavy-metal wastewater (CHMW) requires advanced technology. This study investigated the feasibility of a multi-staged ferrite process (MSFP) for treating CHMW, containing Cd, Cu, Pb, Cr, Zn, Ag, Hg, Ni, Sn and Mn. Our experimental results showed that most of the supernatants after conventional single-step ferrite process could conform to the effluent standard of Environmental Protection Administration in Taiwan. However, the sludge could not satisfy the toxicity characteristic leaching procedure (TCLP) limits due to high Cd, Cu, and Pb concentrations...
March 30, 2012: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/20972860/malignant-solitary-fibrous-tumor-of-the-pleura-computed-tomography-pathological-correlation-and-comparison-with-computed-tomography-of-benign-solitary-fibrous-tumor-of-the-pleura
#11
COMPARATIVE STUDY
Sun Wha Song, Jung Im Jung, Kyo Young Lee, Mi-Young Kim, Seog Hee Park
PURPOSE: The aim of this study was to determine the computed tomography (CT)-pathological correlation of malignant solitary fibrous tumors of the pleura (MSFP) and to compare these findings with CT findings of benign solitary fibrous tumors of the pleura (BSFTP). MATERIALS AND METHODS: We retrospectively identified the clinical, CT, and pathological findings in seven cases of MSFP. There was a CT-pathological correlation for the MSFPs. Additionally, 12 cases of BSFTP from case files were compared with the clinical and CT features of the MSFPs...
October 2010: Japanese Journal of Radiology
https://www.readbyqxmd.com/read/19428213/uplc-q-tofms-ms-as-a-powerful-technique-for-rapid-identification-of-polymethoxylated-flavones-in-fructus-aurantii
#12
Da-Yong Zhou, Xiu-Li Zhang, Qing Xu, Xing-Ya Xue, Fei-Fang Zhang, Xin-Miao Liang
Polymethoxylated flavones (PMFs), as potential cancer chemopreventive agents, are widely distributed in Citrus genus. In this study, a selected ion monitoring-tandem mass (SIM-MS/MS) method for the rapid identification of PMFs in Fructus aurantii (F. aurantii) with ultra-performance liquid chromatography (UPLC) coupled to quadrupole, hybrid orthogonal acceleration time-of-flight tandem mass spectrometer (Q-TOFMS/MS) was proposed. The MS data for candidates, containing accurate mass and isotopic patterns for both precursors and their fragment ions, were acquired selectively...
August 15, 2009: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/18724869/detection-of-genome-wide-polymorphisms-in-the-at-rich-plasmodium-falciparum-genome-using-a-high-density-microarray
#13
Hongying Jiang, Ming Yi, Jianbing Mu, Louie Zhang, Al Ivens, Leszek J Klimczak, Yentram Huyen, Robert M Stephens, Xin-Zhuan Su
BACKGROUND: Genetic mapping is a powerful method to identify mutations that cause drug resistance and other phenotypic changes in the human malaria parasite Plasmodium falciparum. For efficient mapping of a target gene, it is often necessary to genotype a large number of polymorphic markers. Currently, a community effort is underway to collect single nucleotide polymorphisms (SNP) from the parasite genome. Here we evaluate polymorphism detection accuracy of a high-density 'tiling' microarray with 2...
2008: BMC Genomics
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