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https://www.readbyqxmd.com/read/28230089/endothelin-receptor-specific-control-of-endoplasmic-reticulum-stress-and-apoptosis-in-the-kidney
#1
Carmen De Miguel, William C Hamrick, Janet L Hobbs, David M Pollock, Pamela K Carmines, Jennifer S Pollock
Endothelin-1 (ET-1) promotes renal damage during cardiovascular disease; yet, the molecular mechanisms involved remain unknown. Endoplasmic reticulum (ER) stress, triggered by unfolded protein accumulation in the ER, contributes to apoptosis and organ injury. These studies aimed to determine whether the ET-1 system promotes renal ER stress development in response to tunicamycin. ETB deficient (ETB def) or transgenic control (TG-con) rats were used in the presence or absence of ETA receptor antagonism. Tunicamycin treatment similarly increased cortical ER stress markers in both rat genotypes; however, only ETB def rats showed a 14-24 fold increase from baseline for medullary GRP78, sXBP-1, and CHOP...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223691/cellular-glycosylation-affects-herceptin-binding-and-sensitivity-of-breast-cancer-cells-to-doxorubicin-and-growth-factors
#2
Diluka Peiris, Alexander F Spector, Hannah Lomax-Browne, Tayebeh Azimi, Bala Ramesh, Marilena Loizidou, Hazel Welch, Miriam V Dwek
Alterations in protein glycosylation are a key feature of oncogenesis and have been shown to affect cancer cell behaviour perturbing cell adhesion, favouring cell migration and metastasis. This study investigated the effect of N-linked glycosylation on the binding of Herceptin to HER2 protein in breast cancer and on the sensitivity of cancer cells to the chemotherapeutic agent doxorubicin (DXR) and growth factors (EGF and IGF-1). The interaction between Herceptin and recombinant HER2 protein and cancer cell surfaces (on-rate/off-rate) was assessed using a quartz crystal microbalance biosensor revealing an increase in the accessibility of HER2 to Herceptin following deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28215742/identification-of-grass-carp-ctenopharyngodon-idella-xbp1s-as-a-primary-member-in-er-stress
#3
Xiangqin Wang, Yichuan Mi, Bin Zhong, Huiling Mao, Yiqi Wan, Tao Zhang, Haizhou Wang, Chengyu Hu
X-box binding protein 1 (XBP1), a vital basic leucine zipper transcription factor for the related gene transcription in endoplasmic reticulum (ER) stress, belongs to the CREB/ATF family. In mammals, XBP1S is the activated one of XBP1 isoform. In order to study the role of fish XBP1S, we cloned and identified the XBP1S (KU509247) from grass carp (Ctenopharyngodon idella) (named CiXBP1S) by homologous cloning and RACE technique. The full length of CiXBP1S is 1694 bp along with 124 bp of 5' UTR, 418 bp of 3' UTR and the longest open reading frame (1152 bp) encoding a polypeptide of 383 amino acids with a well conserved DNA binding domain (BRLZ domain)...
February 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28202509/sodium-channel-subunit-scnn1b-suppresses-gastric-cancer-growth-and-metastasis-via-grp78-degradation
#4
Jun Yu, Qian Yun, Chi Chun Wong, Jiaying Xu, Huarong Chen, Yanquan Zhang, Wei Kang, Hua Wang, Li Zhang, Weilin Li, Eagle Sh Chu, Minnie Yy Go, Philip Wy Chiu, Enders Kw Ng, Francis Kl Chan, Joseph Jy Sung, Jianmin Si
There remains a paucity of functional biomarkers in gastric cancer. Here we report the identification of the sodium channel subunit SCNN1B as a candidate biomarker in gastric cancer (GC). SCNN1B mRNA expression was silenced commonly by promoter hypermethylation in GC cell lines and primary tumor tissues. Tissue microarray analysis revealed that high expression of SCNN1B was an independent prognostic factor for longer survival in GC patients, especially those with late-stage disease. Functional studies demonstrated that SCNN1B overexpression was sufficient to suppress multiple features of cancer cell pathophysiology in vitro and in vivo...
February 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28181559/sirtuin1-protects-endothelial-caveolin-1-expression-and-preserves-endothelial-function-via-suppressing-mir-204-and-endoplasmic-reticulum-stress
#5
M Kassan, A Vikram, Y R Kim, Q Li, A Kassan, H H Patel, S Kumar, M Gabani, J Liu, J S Jacobs, K Irani
Sirtuin1 (Sirt1) is a class III histone deacetylase that regulates a variety of physiological processes, including endothelial function. Caveolin1 (Cav1) is also an important determinant of endothelial function. We asked if Sirt1 governs endothelial Cav1 and endothelial function by regulating miR-204 expression and endoplasmic reticulum (ER) stress. Knockdown of Sirt1 in endothelial cells, and in vivo deletion of endothelial Sirt1, induced endothelial ER stress and miR-204 expression, reduced Cav1, and impaired endothelium-dependent vasorelaxation...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178380/pre-ischemia-melatonin-treatment-alleviated-acute-neuronal-injury-after-ischemic-stroke-by-inhibiting-er-stress-dependent-autophagy-via-perk-and-ire1-signalings
#6
Dayun Feng, Bao Wang, Lei Wang, Neeta Abraham, Kai Tao, Lu Huang, Wei Shi, Yushu Dong, Yan Qu
Melatonin has demonstrated a potential protective effect in central nervous system. Thus, it is interesting to determine whether pre-ischemia melatonin administration could protect against cerebral ischemia/reperfusion (IR) related injury and the underlying molecular mechanisms. In this study, we revealed that IR injury significantly activated endoplasmic reticulum (ER) stress and autophagy in a middle cerebral artery occlusion (MCAO) mouse model. Pre-ischemia melatonin treatment was able to attenuate IR-induced ER stress and autophagy...
February 8, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28157203/hepatocyte-growth-factor-secreted-by-bone-marrow-stem-cell-reduce-er-stress-and-improves-repair-in-alveolar-epithelial-ii-cells
#7
Izabela Nita, Katrin Hostettler, Luca Tamo, Michaela Medová, Giuseppe Bombaci, Jun Zhong, Ramanjaneyulu Allam, Yitzhak Zimmer, Michael Roth, Thomas Geiser, Amiq Gazdhar
Idiopathic Pulmonary Fibrosis (IPF) is a progressive, irreversible lung disease with complex pathophysiology. Evidence of endoplasmic reticulum (ER) stress has been reported in alveolar epithelial cells (AEC) in IPF patients. Secreted mediators from bone marrow stem cells (BMSC-cm) have regenerative properties. In this study we investigate the beneficial effects of BMSC-cm on ER stress response in primary AEC and ER stressed A549 cells. We hypothesize that BMSC-cm reduces ER stress. Primary AEC isolated from IPF patients were treated with BMSC-cm...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28153729/4-pba-reverses-autophagic-dysfunction-and-improves-insulin-sensitivity-in-adipose-tissue-of-obese-mice-via-akt-mtor-signaling
#8
Qinyue Guo, Lin Xu, Huixia Li, Hongzhi Sun, Shufang Wu, Bo Zhou
BACKGROUND: 4-phenyl butyric acid (4-PBA) has been considered as a key regulator of insulin resistance in obesity. However the mechanism of 4-PBA involved in insulin resistance remains elusive. METHODS: We evaluated the effect of 4-PBA on abnormal autophagy and endoplasmic reticulum (ER) stress in obese mice. 4-PBA was administered in obese mice and adipocyte models, and metabolic parameters, autophagy markers, ER stress indicators, Akt/mTOR signaling and insulin signaling molecular were assessed...
March 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28150859/brain-derived-neurotrophic-factor-improves-proliferation-of-endometrial-epithelial-cells-by-inhibition-of-endoplasmic-reticulum-stress-during-early-pregnancy
#9
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family binds to two transmembrane receptors; neurotrophic receptor tyrosine kinase 2 (NTRK2) with high affinity and p75 with low affinity. Although BDNF-NTRK2 signaling in the central nervous system is known, signaling in the female reproductive system is unknown. Therefore, we determined effects of BDNF on porcine endometrial luminal epithelial (pLE) cells isolated from Day 12 of pregnancy, as well as expression of BDNF and NTRK2 in endometria of cyclic and pregnant pigs...
February 2, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28146117/anti-fibrotic-effect-of-losartan-an-angiotensin-ii-receptor-blocker-is-mediated-through-inhibition-of-er-stress-via-up-regulation-of-sirt1-followed-by-induction-of-ho-1-and-thioredoxin
#10
Hyosang Kim, Chung Hee Baek, Raymond Bok Lee, Jai Won Chang, Won Seok Yang, Sang Koo Lee
Endoplasmic reticulum (ER) stress is increasingly identified as modulator of fibrosis. Losartan, an angiotensin II receptor blocker, has been widely used as the first choice of treatment in chronic renal diseases. We postulated that anti-fibrotic effect of losartan is mediated through inhibition of ER stress via SIRT1 (silent mating type information regulation 2 homolog 1) hemeoxygenase-1 (HO-1)/thioredoxin pathway. Renal tubular cells, tunicamycin (TM)-induced ER stress, and unilateral ureteral obstruction (UUO) mouse model were used...
January 31, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28131804/downregulation-of-autophagy-related-gene-atg5-and-gabarap-expression-by-ifn-%C3%AE-1-contributes-to-its-anti-hcv-activity-in-human-hepatoma-cells
#11
Xu Li, Yu Li, Shoucai Fang, Jinming Su, Junjun Jiang, Bingyu Liang, Jiegang Huang, Bo Zhou, Ning Zang, Wenzhe Ho, Jieliang Li, Yiping Li, Hui Chen, Li Ye, Hao Liang
Type-III interferon (IFN-λ), the most recently discovered family of IFNs, shares common features with type I IFNs, but also has many distinctive activities. It is not clear that whether IFN-λ has additional antiviral mechanisms. In this study, we investigated the effects of IFN-λ on autophagy, a cellular process closely related to hepatitis C virus (HCV) infection in human hepatoma Huh7 cells. Our results showed that IFN-λ1 treatment inhibit autophagic activity in Huh7 cells, as evidenced by the decreased expression of microtubule-associated protein 1 light chain 3B (LC3B)-II and conversion of LC3B-I to LC3B-II, decreased formation of GFP-LC3 puncta and accumulation of autophagosomes...
January 26, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28104755/n-glycosylation-of-human-sphingomyelin-phosphodiesterase-acid-like-3a-smpdl3a-is-essential-for-stability-secretion-and-activity
#12
Mathew Traini, Raani Kumaran, Morten Thaysen-Andersen, Maaike Kockx, Wendy Jessup, Leonard Kritharides
Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a recently identified phosphodiesterase, which is a secreted N -linked glycoprotein. SMPDL3A is highly homologous to acid sphingomyelinase (aSMase), but unlike aSMase can not cleave sphingomyelin. Rather, SMPDL3A hydrolyzes nucleotide tri- and diphosphates and their derivatives. While recent structural studies have shed light on these unexpected substrate preferences, many other aspects of SMPDL3A biology which may give insight into its function in vivo remain obscure...
January 19, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28100484/atf6-knock-down-decreases-apoptosis-arrests-the-s-phase-of-the-cell-cycle-and-increases-steroid-hormone-production-in-mouse-granulosa-cells
#13
Yongjie Xiong, Huatao Chen, Pengfei Lin, Aihua Wang, Lei Wang, Yaping Jin
Activating transcription factor 6 (ATF6) is an important factor in the endoplasmic reticulum (ER) stress signaling pathway. The aim of this study was to assess the role of ATF6 in mouse granulosa cells with respect to apoptosis, the cell cycle, and steroid hormone production, as well as several key genes related to follicular development, via RNA interference, immunohistochemical staining, real-time quantitative polymerase chain reaction, western blotting, flow cytometry, TUNEL assay, and ELISA. Immunohistochemical staining revealed that ATF6 was extensively distributed in the granulosa cells of various ovarian follicles and oocytes in adult female mice...
January 18, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28087337/tunicamycin-impairs-olfactory-learning-and-synaptic-plasticity-in-the-olfactory-bulb
#14
Jia Tong, Fumino Okutani, Yoshihiro Murata, Mutsuo Taniguchi, Toshiharu Namba, Yu-Jie Wang, Hideto Kaba
Tunicamycin (TM) induces endoplasmic reticulum (ER) stress and inhibits N-glycosylation in cells. ER stress is associated with neuronal death in neurodegenerative disorders, such as Parkinson's disease and Alzheimer's disease, and most patients complain of the impairment of olfactory recognition. Here we examined the effects of TM on aversive olfactory learning and the underlying synaptic plasticity in the main olfactory bulb (MOB). Behavioral experiments demonstrated that the intrabulbar infusion of TM disabled aversive olfactory learning without affecting short-term memory...
March 6, 2017: Neuroscience
https://www.readbyqxmd.com/read/28071725/astragalus-polysaccharides-attenuate-pcv2-infection-by-inhibiting-endoplasmic-reticulum-stress-in-vivo-and-in-vitro
#15
Hongxia Xue, Fang Gan, Gang Qian, Junfa Hu, Shu Hao, Jing Xu, Xingxiang Chen, Kehe Huang
This study explored the effects of Astragalus polysaccharide (APS) on porcine circovirus type 2 (PCV2) infections and its mechanism in vivo and vitro. First, fifty 2-week-old mice were randomly divided into five groups: a group without PCV2 infection and groups with PCV2 infections at 0, 100, 200 or 400 mg/kg APS treatments. The trial lasted for 28 days. The results showed that APS treatments at 200 and 400 mg/kg reduced the pathological injury of tissues, inhibited PCV2 infection and decreased glucose-regulated protein 78 (GRP78) and GADD153/CHOP gene mRNA and protein expression significantly (P < 0...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28068312/mray-antibiotic-complex-reveals-details-of-tunicamycin-mode-of-action
#16
Jonna K Hakulinen, Jenny Hering, Gisela Brändén, Hongming Chen, Arjan Snijder, Margareta Ek, Patrik Johansson
The rapid increase of antibiotic resistance has created an urgent need to develop novel antimicrobial agents. Here we describe the crystal structure of the promising bacterial target phospho-N-acetylmuramoyl-pentapeptide translocase (MraY) in complex with the nucleoside antibiotic tunicamycin. The structure not only reveals the mode of action of several related natural-product antibiotics but also gives an indication on the binding mode of the MraY UDP-MurNAc-pentapeptide and undecaprenyl-phosphate substrates...
March 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28067406/ninjurin1-assembles-into-a-homomeric-protein-complex-maintained-by-n-linked-glycosylation
#17
Sung-Jin Bae, Min Wook Shin, Ran Hee Kim, Dongyoon Shin, Taekwon Son, Hee-Jun Wee, Kyu-Won Kim
Ninjurin1 (Ninj1) is a cell surface protein known as a homophilic adhesion molecule. Previous studies have shown a trans-interaction of Ninj1 between immune cells and endothelial cells; however, little is known about Ninj1 modification and structure in the cis-interaction. We showed that Ninj1 assembles into a homomeric complex via a cis-interaction mediated by the intracellular region and N-glycosylation at Asn(60) . We identified cis-interaction between Ninj1 proteins using CFP- and YFP-tagged Ninj1 by Förster resonance energy transfer using a confocal microscope and fluorescence-activated cell sorter...
January 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28063235/a-critical-role-for-adiponectin-mediated-development-of-endometrial-luminal-epithelial-cells-during-the-peri-implantation-period-of-pregnancy
#18
Whasun Lim, Myung Jin Choi, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
Adiponectin is one of the adipokines in the collagen superfamily. It is secreted primarily by white adipocytes and influences reproductive processes including ovarian and uterine functions. Adiponectin regulates energy homeostasis, insulin sensitivity and is anti-inflammatory in various tissues. Its receptors (ADIPOR1 and ADIPOR2) are widely expressed in mammalian tissues, including porcine conceptuses and endometrial during the estrous cycle and peri-implantation period of pregnancy. However, regulatory effects of adiponectin on endometrial epithelial cells are unknown...
January 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28049460/bitter-melon-protects-against-er-stress-in-ls174t-colonic-epithelial-cells
#19
Dale A Kunde, Wai Chin Chong, Prathiba V Nerurkar, Kiran D K Ahuja, Jeremy Just, Jason A Smith, Nuri Guven, Rajaraman D Eri
BACKGROUND: Bitter Melon (BM) has been used as a functional food in traditional Chinese and Indian medicine for many generations and has gained a great deal of attention due to its apparent benefits in moderating some of the pathogenic processes in a variety of inflammatory conditions. BM extract (BME) has been shown to possess strong anti-oxidant properties. In addition, it can ameliorate oxidative stress and potentially ER stress. There is increasing evidence that oxidative and ER stress are major contributors for intestinal secretory cell dysfunction which leads to local inflammation and disease pathogenesis that are hallmarks of inflammatory bowel diseases (IBD)...
January 3, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28039489/trail-receptor-gene-editing-unveils-trail-r1-as-a-master-player-of-apoptosis-induced-by-trail-and-er-stress
#20
Florent Dufour, Thibault Rattier, Andrei Alexandru Constantinescu, Luciana Zischler, Aymeric Morlé, Hazem Ben Mabrouk, Etienne Humblin, Guillaume Jacquemin, Eva Szegezdi, Fabien Delacote, Naziha Marrakchi, Gilles Guichard, Catherine Pellat-Deceunynck, Pierre Vacher, Patrick Legembre, Carmen Garrido, Olivier Micheau
TRAIL induces selective tumor cell death through TRAIL-R1 and TRAIL-R2. Despite the fact that these receptors share high structural homologies, induction of apoptosis upon ER stress, cell autonomous motility and invasion have solely been described to occur through TRAIL-R2. Using the TALEN gene-editing approach, we show that TRAIL-R1 can also induce apoptosis during unresolved unfolded protein response (UPR). Likewise, TRAIL-R1 was found to co-immunoprecipitate with FADD and caspase-8 during ER stress. Its deficiency conferred resistance to apoptosis induced by thaspigargin, tunicamycin or brefeldin A...
February 7, 2017: Oncotarget
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