keyword
MENU ▼
Read by QxMD icon Read
search

tunicamycin

keyword
https://www.readbyqxmd.com/read/29154199/ultraviolet-b-radiation-down-regulates-ulk1-and-atg7-expression-and-impairs-the-autophagy-response-in-human-keratinocytes
#1
Xu Chen, Li Li, Song Xu, Wenbo Bu, Kun Chen, Min Li, Heng Gu
Autophagy is a self-digestive pathway that helps to maintain cellular homeostasis, and many autophagy-related gene (ATG)s involved the regulation of the autophagy process. Ultraviolet light is a common stressor of skin, but it is unclear how autophagy is regulated after ultraviolet exposure in epidermal keratinocytes. Here, we found that the mRNAs of some key ATG genes such as ULK1, ATG5 and ATG7 exhibited significantly lower levels in the skin tissues of the face and chest with solar ultraviolet exposure, compared with perineal skin...
September 4, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29149522/melatonin-improves-the-meiotic-maturation-of-porcine-oocytes-by-reducing-endoplasmic-reticulum-stress-during-in-vitro-maturation
#2
Hyo-Jin Park, Jae-Young Park, Jin-Woo Kim, Seul-Gi Yang, Jae-Min Jung, Min-Ji Kim, Man-Jong Kang, Young Ho Cho, Gabbine Wee, Hee-Young Yang, Bong-Seok Song, Sun-Uk Kim, Deog-Bon Koo
Under endoplasmic reticulum (ER)-stress conditions, the unfolded protein response (UPR) generates a defense mechanism in mammalian cells. The regulation of UPR signaling is important in oocyte maturation, embryo development, and female reproduction of pigs. Recent studies have shown that melatonin plays an important role as an anti-oxidant to improve pig oocyte maturation. However, there is no report on the role of melatonin in the regulation of UPR signaling and ER-stress during in vitro maturation (IVM) of porcine oocytes...
November 17, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/29134561/cannabinoid-receptor-type-1-agonist-acea-protects-neurons-from-death-and-attenuates-endoplasmic-reticulum-stress-related-apoptotic-pathway-signaling
#3
Talita A Vrechi, Fernanda Crunfli, Andressa P Costa, Andréa S Torrão
Neurodegeneration is the result of progressive destruction of neurons in the central nervous system, with unknown causes and pathological mechanisms not yet fully elucidated. Several factors contribute to neurodegenerative processes, including neuroinflammation, accumulation of neurotoxic factors, and misfolded proteins in the lumen of the endoplasmic reticulum (ER). Endocannabinoid signaling has been pointed out as an important modulatory system in several neurodegeneration-related processes, inhibiting the inflammatory response and increasing neuronal survival...
November 13, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29130967/endoplasmic-reticulum-stress-promotes-autophagy-and-apoptosis-and-reduces-chemotherapy-resistance-in-mutant-p53-lung-cancer-cells
#4
Ping-Ping Gan, Yang-Ying Zhou, Mei-Zuo Zhong, Yun Peng, Li Li, Jian-Huang Li
BACKGROUND/AIMS: Lung cancer (LC) continues to be one of the most prevalent cancers around the world. During this study we aimed to investigate the involvement of endoplasmic reticulum stress (ERS) in autophagy, apoptosis, and chemotherapy resistance of mutant p53 LC cells. METHODS: Immunohistochemistry was employed to help determine the p53 mutation status of cancer cells from 92 primary LC patients, who were subsequently assigned to either the mutant p53 (n = 39) or wild-type p53 group (n = 53)...
November 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29122482/indirubin-derivatives-protect-against-endoplasmic-reticulum-stress-induced-cytotoxicity-and-down-regulate-chop-levels-in-ht22-cells
#5
Yasuhiro Kosuge, Hiroaki Saito, Tatsuki Haraguchi, Yoshimi Ichimaru, Sachiyo Ohashi, Hiroko Miyagishi, Shunsuke Kobayashi, Kumiko Ishige, Shinichi Miyairi, Yoshihisa Ito
Indirubin and its derivatives have been reported to exhibit anti-cancer and anti-inflammatory activities. Recently, some of its derived analogs have been shown to have neuroprotective potential. Endoplasmic reticulum (ER) stress has been demonstrated to contribute to the pathogenesis of various neurodegenerative diseases, whereas the effects of indirubin derivatives on ER stress-induced cell death have not been addressed. In the present study, a series of 44 derivatives of indirubin was prepared to search for a novel class of neuroprotective agents against ER stress-induced neuronal death...
October 28, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29111387/hyperlipidemia-induced-hepassocin-in-the-liver-contributes-to-insulin-resistance-in-skeletal-muscle
#6
Tae Woo Jung, Yoon Hee Chung, Hyoung-Chun Kim, A M Abd El-Aty, Ji Hoon Jeong
Hepassocin (HPS) has recently been identified as a novel hepatokine that causes hepatic steatosis. However, the role of HPS in the development of insulin resistance in skeletal muscle under obesity remains unclear. The effect of hyperlipidemia on hepatic HPS expression was evaluated in primary hepatocytes and liver of mice. HPS-mediated signal pathways were explored using small interfering (si) RNAs of specific genes or inhibitors. We found that treatment of primary hepatocytes with palmitate could induce HPS expression through C/EBPβ-mediated transcriptional activation...
October 27, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29109149/chronic-oxidative-stress-promotes-gadd34-mediated-phosphorylation-of-the-tar-dna-binding-protein-tdp-43-a-modification-linked-to-neurodegeneration
#7
Catherine Wenhui Goh, Irene Chengjie Lee, Jeyapriya Rajameenakshi Sundaram, Simi Elizabeth George, Permeen Yusoff, Matthew Hayden Brush, Newman Siu Kwan Sze, Shirish Shenolikar
Oxidative and endoplasmic reticulum (ER) stresses are hallmarks of the pathophysiology of amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases. In these stresses, different kinases phosphorylate eukaryotic initiation factor eIF2α enabling the translation of stress response genes, among these is GADD34, the protein product of which recruits the α-isoform of protein phosphatase 1 catalytic subunit (PP1α) and eIF2α to assemble a phosphatase complex catalyzing eIF2α dephosphorylation and resumption of protein synthesis...
November 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29107182/identification-of-the-internal-ribosome-entry-sites-ires-of-prion-protein-gene
#8
Xiao-Nuan Luo, Qin-Qin Song, Jie Yu, Juan Song, Xin-Ling Wang, Dong Xia, Peng Sun, Jun Han
Many studies demonstrated that there are several type bands of prion protein in cells. However, the formation of different prion protein bands is elusive. After several low molecular weight bands of prion protein appeared in SMB-S15 cells infected with scrapie agent Chandler, we think that IRES-dependent translation mechanism induced by prion is involved in the formation of prion protein bands. Then we designed a series of pPrP-GFP fusing plasmids and bicistronic plasmids to identify the IRES sites of prion protein gene and found 3 IRES sites inside of PrP mRNA...
October 26, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29105510/microrna-29a-mitigation-of-endoplasmic-reticulum-and-autophagy-aberrance-counteracts-in-obstructive-jaundice-induced-fibrosis-in-mice
#9
Ying-Hsien Huang, Ya-Ling Yang, Fu-Chen Huang, Mao-Meng Tiao, Yen-Cheng Lin, Ming-Horng Tsai, Feng-Sheng Wang
Hepatic fibrosis was caused by a number of signaling pathways that damage liver integrity. We have previously shown that microRNA-29a (miR-29a) protects against liver fibrosis. Aberrant endoplasmic reticulum (ER) and autophagy function reportedly exaggerate hepatic disorders. The aim of this study was to characterize the biological influence of miR-29a on ER function in injured livers with bile duct ligation (BDL). We performed BDL on miR-29a transgenic mice (miR-29aTg) and wild-type mice to induce cholestatic liver injury...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29097707/gp73-regulates-hepatic-steatosis-by-enhancing-scap-srebps-interaction
#10
Xiaoli Yang, Feixiang Wu, Jiankang Chen, Cui Wang, Yongjie Zhu, Feng Li, Qinfang Hao, Cuijuan Duan, Li Wang, Xueping Ma, Deyong Zou, Li Luo, Yiwen Zhao, Kai Guan, Yuan Cao, Pingping Zhang, Pengyu Zhou, Shengli Ma, Zhifeng Yan, Jia Li, Yanhong Zhang, Congwen Wei, Hui Zhong
Elevated Golgi phosphoprotein 2 (GP73, also known as GOLPH2 or GOLM1) expression in serum and liver, which can be induced by viral infection and cytokine treatments, is intimately connected with liver disease, including acute hepatitis, cirrhosis and hepatocellular carcinoma (HCC). However, its pathogenic roles in hepatic diseases have never been clarified in detail. Here, we showed that the upregulated GP73 is indispensable for SREBPs activation and lipogenesis. Notably, GP73 overexpression enhanced SCAP-SREBPs binding and its Golgi trafficking even under cholesterol sufficiency...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29095946/endoplasmic-reticulum-stress-inhibits-expression-of-genes-involved-in-thyroid-hormone-synthesis-and-their-key-transcriptional-regulators-in-frtl-5-thyrocytes
#11
Gaiping Wen, Robert Ringseis, Klaus Eder
Endoplasmic reticulum (ER) stress is characterized by the accumulation of misfolded proteins due to an impairment of ER quality control pathways leading to the activation of a defense system, called unfolded protein response (UPR). While thyrocytes are supposed to be highly susceptible to environmental conditions that cause ER stress due to the synthesis of large amounts of secretory proteins required for thyroid hormone synthesis, systematic investigations on the effect of ER stress on expression of key genes of thyroid hormone synthesis and their transcriptional regulators are lacking...
2017: PloS One
https://www.readbyqxmd.com/read/29089601/selective-catalytic-hydrogenation-of-the-n-acyl-and-uridyl-double-bonds-in-the-tunicamycin-family-of-protein-n-glycosylation-inhibitors
#12
Neil Pj Price, Michael A Jackson, Karl E Vermillion, Judith A Blackburn, Jiakun Li, Biao Yu
Tunicamycin is a Streptomyces-derived inhibitor of eukaryotic protein N-glycosylation and bacterial cell wall biosynthesis, and is a potent and general toxin by these biological mechanisms. The antibacterial activity is dependent in part upon a π-π stacking interaction between the tunicamycin uridyl group and a specific Phe residue within MraY, a tunicamycin-binding protein in bacteria. We have previously shown that reducing the tunicamycin uridyl group to 5,6-dihydrouridyl (DHU) significantly lowers its eukaryotic toxicity, potentially by disrupting the π-stacking with the active site Phe...
November 1, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/29081452/reduced-glutathione-alleviates-tunicamycin-induced-endoplasmic-reticulum-stress-in-mouse-preimplantation-embryos
#13
Ihsan Ali, Hai Xing Liu, Li Zhong-Shu, Ma Dong-Xue, Lijie Xu, Syed Zahid Ali Shah, Obaid Ullah, Fang Nan-Zhu
Endoplasmic reticulum (ER) stress, a dysfunction in protein-folding capacity, is involved in many pathological and physiological responses, including embryonic development. This study aims to determine the developmental competence, apoptosis, and stress-induced gene expression in mouse preimplantation embryos grown in an in vitro culture medium supplemented with different concentrations of the ER stress inducer tunicamycin (TM) and the antioxidant glutathione (GSH). Treatment of zygotes with 0.5 µg/ml TM significantly decreased (P < 0...
October 27, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29079428/globular-adiponectin-protects-hepatocytes-from-tunicamycin-induced-cell-death-via-modulation-of-the-inflammasome-and-heme-oxygenase-1-induction
#14
Amrita Khakurel, Pil-Hoon Park
Endoplasmic reticulum (ER) stress, which is defined as the accumulation of unfolded or misfolded proteins in the ER, triggers cellular dysfunction and eventually leads to cell death. In particular, excessive and prolonged ER stress is closely related with hepatic injury. Adiponectin, a hormone predominantly produced by adipose tissue, is known to possess potent hepatoprotective properties and exhibits a cytoprotective effect in response to chronic ER stress. However, the underlying mechanisms are not clearly understood...
October 24, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29066182/tetramethylpyrazine-suppresses-angiotensin-ii-induced-soluble-epoxide-hydrolase-expression-in-coronary-endothelium-via-anti-er-stress-mechanism
#15
Shiu-Kwong Mak, Cheuk-Man Yu, Wen-Tao Sun, Guo-Wei He, Xiao-Cheng Liu, Qin Yang
Activation of soluble epoxide hydrolase (sEH) is associated with endothelial dysfunction in hypertension, though the underlying mechanisms are inadequately understood and the role of endoplasmic reticulum (ER) stress is yet to be studied in detail. Tetramethylpyrazine (TMP), a major bioactive ingredient of Chinese herb Chuanxiong, is well-known for its cardiovascular benefits. Nevertheless, whether TMP may protect vascular endothelium from ER stress and whether regulation of sEH is involved remain unknown. This study aimed at investigating the role of ER stress in angiotensin-II (Ang-II)-induced sEH dysregulation and elucidating the significance of ER stress regulation in the vasoprotective effect of TMP...
October 21, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29027487/effect-of-tauroursodeoxycholic-acid-on-pufa-levels-and-inflammation-in-an-animal-and-cell-model-of-hepatic-endoplasmic-reticulum-stress
#16
M Aslan, E Kıraç, Ö Yılmaz, B Ünal, E K Konuk, F Özcan, H Tuzcu
The aim of this study was to evaluate hepatic polyunsaturated fatty acids (PUFAs) and inflammatory response in an animal and cell model of endoplasmic reticulum (ER) stress. Rats were divided into control, tunicamycin (TM)-treated, and TM + tauroursodeoxycholic acid (TUDCA)-treated groups. Hepatic ER stress was induced by TM and the ER stress inhibitor TUDCA was injected 30 min before induction of ER stress. Liver THLE-3 cells were treated with TM and TUDCA was administered in advance to decrease cytotoxic effects...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29023770/c-c-motif-chemokine-ligand-23-abolishes-er-stress-and-lps-induced-reduction-in-proliferation-of-bovine-endometrial-epithelial-cells
#17
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
To reduce embryonic losses in domestic animals for economic production of livestock meat and milk, chemokines and their receptors are required for proper implantation and placentation during early pregnancy. In this study, we investigated the effects of C-C-motif chemokine ligand 23 (CCL23) on the proliferation of bovine endometrial (BEND) epithelial cells. CCL23 treatment improved BEND cell proliferation by enhancing PCNA and cyclin D1 expression via activation of the PI3K/AKT and MAPK signaling pathways. In addition, a combination of CCL23 and tunicamycin reversed the ER stress-induced reduction in cell proliferation and the decreased expression of UPR-mediated signaling proteins, including IRE1α, PERK, and ATF6α...
October 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28987725/mechanical-and-hypoxia-stress-can-cause-chondrocytes-apoptosis-through-over-activation-of-endoplasmic-reticulum-stress
#18
Ziwei Huang, Min Zhou, Qian Wang, Mengjiao Zhu, Sheng Chen, Huang Li
OBJECTIVE: To examine the role of mechanical force and hypoxia on chondrocytes apoptosis and osteoarthritis (OA)-liked pathological change on mandibular cartilage through over-activation of endoplasmic reticulum stress (ERS). METHODS: We used two in vitro models to examine the effect of mechanical force and hypoxia on chondrocytes apoptosis separately. The mandibular condylar chondrocytes were obtained from three-week-old male Sprague-Dawley rats. Flexcell 5000T apparatus was used to produce mechanical forces (12%, 0...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28980921/protease-sensitivity-and-redistribution-of-cd71-and-glycophorin-a-on-k562-cells
#19
C-H Yu, H Wang, Y Wang, N-X Cui, X Zhao, L Rong, Z-C Yi
Transmembrane proteins are delivered to plasma membrane from the endoplasmic reticulum and Golgi complex by vesicular transport along with the cytoskeletal network. Disruption of this process likely affects transmembrane protein expression. K562 cells were digested with Streptomyces griseus protease for different periods of time, and then re-cultured with different cytoskeletal and glycosylation inhibitors. Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by flow cytometry...
September 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28978108/tunicamycin-induced-endoplasmic-reticulum-stress-promotes-apoptosis-of-prostate-cancer-cells-by-activating-mtorc1
#20
Prasun Guha, Engin Kaptan, Padmaja Gade, Dhananjaya V Kalvakolanu, Hafiz Ahmed
Studies suggest that tunicamycin may work as a therapeutic drug to cancer cells by inducing stress in the endoplasmic reticulum (ER) through unfolded protein response (UPR) and thereby promoting apoptosis. However, mechanisms of the prolonged activation of the UPR under sustained ER stress in the regulation of cell apoptosis are largely unknown. To delineate the role of candidate genes in the apoptotic process under ER stress and to search for new therapeutic strategies to treat metastatic castration resistant prostate cancer, we performed whole genome expression microarray analysis in tunicamycin treated metastatic androgen-insensitive prostate cancer cells, PC-3...
September 15, 2017: Oncotarget
keyword
keyword
80827
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"