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https://www.readbyqxmd.com/read/28336294/palmitate-mediated-diacylglycerol-accumulation-causes-endoplasmic-reticulum-stress-plin2-degradation-and-cell-death-in-h9c2-cardiomyoblasts
#1
Ali Akoumi, Taha Haffar, Maria Mousterji, Robert Scott Kiss, Nicolas Bousette
We have previously shown that palmitate causes ER stress in primary cardiomyocytes and this was associated with a diffuse lipid staining histology. In contrast, oleate, which was non-toxic, led to the formation of abundant, clearly delineated lipid droplets. The aberrant lipid histology in palmitate treated cells led us to hypothesize that perhaps there was an impairment in lipid droplet formation, which could lead to accumulation of lipids in the ER and consequent ER stress. To test this hypothesis we treated H9C2s (a cardiomyoblast cell line) with either 300µM oleate or palmitate for 8h...
March 21, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28333839/evidence-for-increased-response-to-induced-endoplasmic-reticulum-stress-in-myeloid-cells-in-acquired-aplastic-anemia
#2
Alpa Sidhu, Michael U Callaghan, Manisha S Gadgeel, Steven A Buck, Andrew M Fribley, Süreyya Savaşan
Autoimmune response targeting the hematopoietic stem cells highlights the current understanding of acquired aplastic anemia (AAA) pathogenesis. Upregulation of the unfolded protein response is the cell's rejoinder to a variety of stresses, which either result in restoring homeostasis or cell death by increased expression of the transcription factor C/EBP homologous protein. We hypothesized that there is an inherent increased sensitivity to various cellular stressors, including the ones that target endoplasmic reticulum (ER) in AAA leading to a decreased proliferation and potentially contributing to susceptibility to autologous cytotoxicity...
April 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28331281/the-involvement-of-atf4-and-s-opsin-in-retinal-photoreceptor-cell-damage-induced-by-blue-led-light
#3
Emi Ooe, Kazuhiro Tsuruma, Yoshiki Kuse, Saori Kobayashi, Masamitsu Shimazawa, Hideaki Hara
PURPOSE: Blue light is a high-energy emitting light with a short wavelength in the visible light spectrum. Blue light induces photoreceptor apoptosis and causes age-related macular degeneration or retinitis pigmentosa. In the present study, we investigated the roles of endoplasmic reticulum (ER) stress induced by blue light-emitting diode (LED) light exposure in murine photoreceptor cells. METHODS: The murine photoreceptor cell line was incubated and exposed to blue LED light (464 nm blue LED light, 450 lx, 3 to 24 h)...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28322783/elevation-of-liver-endoplasmic-reticulum-stress-in-a-modified-choline-deficient-l-amino-acid-defined-diet-fed-non-alcoholic-steatohepatitis-mouse-model
#4
Yo Muraki, Yukimasa Makita, Midori Yamasaki, Yuichiro Amano, Takanori Matsuo
Endoplasmic reticulum (ER) stress caused by accumulation of misfolded proteins is observed in several kinds of diseases. Since ER stress is reported to be involved in the progression of non-alcoholic steatohepatitis (NASH), highly sensitive and simple measurement methods are required for research into developing novel therapy for NASH. To investigate the involvement of ER stress in NASH pathogenesis in a mouse model, an assay for liver ER stress was developed using ER stress activated indicator-luciferase (ERAI-Luc) mice...
March 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28303141/the-function-of-fk506-binding-protein-13-in-protein-quality-control-protects-plasma-cells-from-endoplasmic-reticulum-stress-associated-apoptosis
#5
Mini Jeong, Eunkyeong Jang, Suk San Choi, Changhoon Ji, Kyungho Lee, Jeehee Youn
Plasma cells (PCs) are exposed to intense endoplasmic reticulum (ER) stress imposed by enormous rates of immunoglobulin (Ig) synthesis and secretion. Therefore, protein homeostasis is crucial for the survival of PCs, but its molecular mechanism remains largely unknown. Here, we found marked overexpression of FK506-binding protein 13 (FKBP13) in long-lived PCs from autoimmune mice and investigated its function using a plasmacytoma cell line secreting IgA. FKBP13 expression was induced largely in the lumen of ER in response to treatment with an ER stressor tunicamycin or overexpression of an adaptive unfolded protein response (UPR) protein X-box binding protein 1 (XBP1)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28298914/endoplasmic-reticulum-stress-response-in-arabidopsis-roots
#6
Yueh Cho, Kazue Kanehara
Roots are the frontier of plant body to perceive underground environmental change. Endoplasmic reticulum (ER) stress response represents circumvention of cellular stress caused by various environmental changes; however, a limited number of studies are available on the ER stress responses in roots. Here, we report the tunicamycin (TM) -induced ER stress response in Arabidopsis roots by monitoring expression patterns of immunoglobulin-binding protein 3 (BiP3), a representative marker for the response. Roots promptly responded to the TM-induced ER stress through the induction of similar sets of ER stress-responsive genes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28286511/bhbzip60-from-resurrection-plant-boea-hygrometrica-is-an-mrna-splicing-activated-endoplasmic-reticulum-stress-regulator-involved-in-drought-tolerance
#7
Bo Wang, Hong Du, Zhennan Zhang, Wenzhong Xu, Xin Deng
Adverse environmental conditions cause endoplasmic reticulum (ER) stress in plants. To mitigate ER stress damage, ER associated transcription factors and inositol-requiring enzyme-1 (IRE1)-mediated bZIP60 mRNA splicing are activated in plants. A drought-induced gene, encoding the ortholog of AtbZIP60, was identified in the resurrection plant Boea hygrometrica, termed BhbZIP60. In response to ER stress and dehydration, BhbZIP60 mRNA can be spliced to create a frame shift in the C terminus by the excision of 23b segment in a manner of its ortholog in other plants, thus translocating to the nucleus instead of the cytoplasm...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28277743/obesity-induced-endoplasmic-reticulum-stress-causes-lung-endothelial-dysfunction-and-promotes-acute-lung-injury
#8
Dilip Shah, Freddy Romero, Zhi Guo, Jianxin Sun, Jonathan Li, Caleb B Kallen, Ulhas P Naik, Ross Summer
Obesity is a significant risk factor for the acute respiratory distress syndrome (ARDS). The mechanisms underlying this association are unknown. We recently showed that diet-induced obese (DIO) mice exhibit pulmonary vascular endothelial dysfunction which is associated with enhanced susceptibility to lipopolysaccharide (LPS)-induced lung injury. Here, we demonstrate that lung endothelial dysfunction in DIO mice coincides with increased endoplasmic reticulum (ER) stress. Specifically, we observed enhanced expression of the major sensors of misfolded proteins including PERK, IREα and ATF6, in whole lung and in lung endothelial cells isolated from DIO mice...
March 9, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28276509/suppression-of-autophagy-by-mycophenolic-acid-contributes-to-inhibition-of-hcv-replication-in-human-hepatoma-cells
#9
Shoucai Fang, Jinming Su, Bingyu Liang, Xu Li, Yu Li, Junjun Jiang, Jiegang Huang, Bo Zhou, Chuanyi Ning, Jieliang Li, Wenzhe Ho, Yiping Li, Hui Chen, Hao Liang, Li Ye
Previous studies have shown that mycophenolic acid (MPA) has an anti-HCV activity. However, the mechanism of MPA-mediated inhibition of HCV replication remains to be determined. This study investigated whether MPA has an effect on autophagy, a cellular machinery required for HCV replication, thereby, inhibits HCV replication in Huh7 cells. MPA treatment of Huh7 cells could suppress autophagy, evidenced by decreased LC3B-II level and conversion of LC3B-I to LC3B-II, decreased autophagosome formation, and increased p62 level compared to MPA-untreated cells...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28275095/bap1-inhibits-the-er-stress-gene-regulatory-network-and-modulates-metabolic-stress-response
#10
Fangyan Dai, Hyemin Lee, Yilei Zhang, Li Zhuang, Hui Yao, Yuanxin Xi, Zhen-Dong Xiao, M James You, Wei Li, Xiaoping Su, Boyi Gan
The endoplasmic reticulum (ER) is classically linked to metabolic homeostasis via the activation of unfolded protein response (UPR), which is instructed by multiple transcriptional regulatory cascades. BRCA1 associated protein 1 (BAP1) is a tumor suppressor with de-ubiquitinating enzyme activity and has been implicated in chromatin regulation of gene expression. Here we show that BAP1 inhibits cell death induced by unresolved metabolic stress. This prosurvival role of BAP1 depends on its de-ubiquitinating activity and correlates with its ability to dampen the metabolic stress-induced UPR transcriptional network...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28263879/ctenopharyngodon-idella-irf2-and-atf4-down-regulate-the-transcriptional-level-of-prkra
#11
Keyi Huang, Guoqin Qi, Zhicheng Sun, Xiancheng Liu, Xiaowen Xu, Haizhou Wang, Zhen Wu, Yiqi Wan, Chengyu Hu
PRKRA (interferon-inducible double-stranded RNA-dependent protein kinase activator A) is a protective protein which regulates the adaptation of cells to ER stress and virus-stimulated signaling pathways by activating PKR. In the present study, a grass carp (Ctenopharyngodon idella) PRKRA full-length cDNA (named CiPRKRA, KT891991) was cloned and identified. The full-length cDNA is comprised of a 5' UTR (36 bp), a 3' UTR (350 bp) and the longest ORF (882 bp) encoding a polypeptide of 293 amino acids. The deduced amino acid sequence of CiPRKRA contains three typical dsRNA binding motifs (dsRBM)...
March 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28251705/selenium-supplementation-modulates-apoptotic-processes-in-thyroid-follicular-cells
#12
Immacolata C Nettore, Emma De Nisco, Silvio Desiderio, Carmela Passaro, Luigi Maione, Mariarosaria Negri, Luigi Albano, Rosario Pivonello, Claudia Pivonello, Giuseppe Portella, Paola Ungaro, Annamaria Colao, Paolo E Macchia
Selenium (Se) is an essential micronutrient modulating several physiopathological processes in the human body. The aim of the study is to characterize the molecular effects determined by Se-supplementation in thyroid follicular cells, using as model the well-differentiated rat thyroid follicular cell line FRTL5. Experiments have been performed to evaluate the effects of Se on cell growth, mortality and proliferation and on modulation of pro- and antiapoptotic pathways. The results indicate that Se-supplementation improves FRTL5 growth rate...
March 2, 2017: BioFactors
https://www.readbyqxmd.com/read/28246472/melatonin-a-novel-selective-atf-6-inhibitor-induces-human-hepatoma-cell-apoptosis-through-cox-2-downregulation
#13
Li-Jia Bu, Han-Qing Yu, Lu-Lu Fan, Xiao-Qiu Li, Fang Wang, Jia-Tao Liu, Fei Zhong, Cong-Jun Zhang, Wei Wei, Hua Wang, Guo-Ping Sun
AIM: To clarify the mechanisms involved in the critical endoplasmic reticulum (ER) stress initiating unfolded protein response pathway modified by melatonin. METHODS: Hepatoma cells, HepG2, were cultured in vitro. Flow cytometry and TUNEL assay were used to measure HepG2 cell apoptosis. Western blotting and quantitative reverse transcription-polymerase chain reaction methods were used to determine the protein and messenger RNA levels of ER stress and apoptosis related genes' expression, respectively...
February 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28246389/undercarboxylated-osteocalcin-reverts-insulin-resistance-induced-by-endoplasmic-reticulum-stress-in-human-umbilical-vein-endothelial-cells
#14
Qinyue Guo, Huixia Li, Lin Xu, Shufang Wu, Hongzhi Sun, Bo Zhou
Osteocalcin has been considered to be an important regulator of energy metabolism in type 2 diabetes mellitus (T2DM). However, the mechanism underlying the involvement of uncarboxylated osteocalcin in the vascular complications of T2DM is not fully understood. In the present study, we analyzed the potential correlations between uncarboxylated osteocalcin and macro- or microangiopathic complications in subjects with T2DM and tested the impact of uncarboxylated osteocalcin on insulin resistance in human umbilical vein endothelial cells (HUVECs)...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28230089/endothelin-receptor-specific-control-of-endoplasmic-reticulum-stress-and-apoptosis-in-the-kidney
#15
Carmen De Miguel, William C Hamrick, Janet L Hobbs, David M Pollock, Pamela K Carmines, Jennifer S Pollock
Endothelin-1 (ET-1) promotes renal damage during cardiovascular disease; yet, the molecular mechanisms involved remain unknown. Endoplasmic reticulum (ER) stress, triggered by unfolded protein accumulation in the ER, contributes to apoptosis and organ injury. These studies aimed to determine whether the ET-1 system promotes renal ER stress development in response to tunicamycin. ETB deficient (ETB def) or transgenic control (TG-con) rats were used in the presence or absence of ETA receptor antagonism. Tunicamycin treatment similarly increased cortical ER stress markers in both rat genotypes; however, only ETB def rats showed a 14-24 fold increase from baseline for medullary GRP78, sXBP-1, and CHOP...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223691/cellular-glycosylation-affects-herceptin-binding-and-sensitivity-of-breast-cancer-cells-to-doxorubicin-and-growth-factors
#16
Diluka Peiris, Alexander F Spector, Hannah Lomax-Browne, Tayebeh Azimi, Bala Ramesh, Marilena Loizidou, Hazel Welch, Miriam V Dwek
Alterations in protein glycosylation are a key feature of oncogenesis and have been shown to affect cancer cell behaviour perturbing cell adhesion, favouring cell migration and metastasis. This study investigated the effect of N-linked glycosylation on the binding of Herceptin to HER2 protein in breast cancer and on the sensitivity of cancer cells to the chemotherapeutic agent doxorubicin (DXR) and growth factors (EGF and IGF-1). The interaction between Herceptin and recombinant HER2 protein and cancer cell surfaces (on-rate/off-rate) was assessed using a quartz crystal microbalance biosensor revealing an increase in the accessibility of HER2 to Herceptin following deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28215742/identification-of-grass-carp-ctenopharyngodon-idella-xbp1s-as-a-primary-member-in-er-stress
#17
Xiangqin Wang, Yichuan Mi, Bin Zhong, Huiling Mao, Yiqi Wan, Tao Zhang, Haizhou Wang, Chengyu Hu
X-box binding protein 1 (XBP1), a vital basic leucine zipper transcription factor for the related gene transcription in endoplasmic reticulum (ER) stress, belongs to the CREB/ATF family. In mammals, XBP1S is the activated one of XBP1 isoform. In order to study the role of fish XBP1S, we cloned and identified the XBP1S (KU509247) from grass carp (Ctenopharyngodon idella) (named CiXBP1S) by homologous cloning and RACE technique. The full length of CiXBP1S is 1694 bp along with 124 bp of 5' UTR, 418 bp of 3' UTR and the longest open reading frame (1152 bp) encoding a polypeptide of 383 amino acids with a well conserved DNA binding domain (BRLZ domain)...
February 20, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28202509/sodium-channel-subunit-scnn1b-suppresses-gastric-cancer-growth-and-metastasis-via-grp78-degradation
#18
Yun Qian, Chi Chun Wong, Jiaying Xu, Huarong Chen, Yanquan Zhang, Wei Kang, Li Zhang, Weilin Li, Hua Wang, Eagle Sh Chu, Enders Kw Ng, Minnie Yy Go, Philip Wy Chiu, Francis Kl Chan, Joseph Jy Sung, Jianmin Si, Jun Yu
There remains a paucity of functional biomarkers in gastric cancer. Here we report the identification of the sodium channel subunit SCNN1B as a candidate biomarker in gastric cancer (GC). SCNN1B mRNA expression was silenced commonly by promoter hypermethylation in GC cell lines and primary tumor tissues. Tissue microarray analysis revealed that high expression of SCNN1B was an independent prognostic factor for longer survival in GC patients, especially those with late-stage disease. Functional studies demonstrated that SCNN1B overexpression was sufficient to suppress multiple features of cancer cell pathophysiology in vitro and in vivo...
February 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28181559/sirtuin1-protects-endothelial-caveolin-1-expression-and-preserves-endothelial-function-via-suppressing-mir-204-and-endoplasmic-reticulum-stress
#19
M Kassan, A Vikram, Y R Kim, Q Li, A Kassan, H H Patel, S Kumar, M Gabani, J Liu, J S Jacobs, K Irani
Sirtuin1 (Sirt1) is a class III histone deacetylase that regulates a variety of physiological processes, including endothelial function. Caveolin1 (Cav1) is also an important determinant of endothelial function. We asked if Sirt1 governs endothelial Cav1 and endothelial function by regulating miR-204 expression and endoplasmic reticulum (ER) stress. Knockdown of Sirt1 in endothelial cells, and in vivo deletion of endothelial Sirt1, induced endothelial ER stress and miR-204 expression, reduced Cav1, and impaired endothelium-dependent vasorelaxation...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178380/pre-ischemia-melatonin-treatment-alleviated-acute-neuronal-injury-after-ischemic-stroke-by-inhibiting-endoplasmic-reticulum-stress-dependent-autophagy-via-perk-and-ire1-signalings
#20
Dayun Feng, Bao Wang, Lei Wang, Neeta Abraham, Kai Tao, Lu Huang, Wei Shi, Yushu Dong, Yan Qu
Melatonin has demonstrated a potential protective effect in central nervous system. Thus, it is interesting to determine whether pre-ischemia melatonin administration could protect against cerebral ischemia/reperfusion (IR)-related injury and the underlying molecular mechanisms. In this study, we revealed that IR injury significantly activated endoplasmic reticulum (ER) stress and autophagy in a middle cerebral artery occlusion mouse model. Pre-ischemia melatonin treatment was able to attenuate IR-induced ER stress and autophagy...
February 8, 2017: Journal of Pineal Research
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