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https://www.readbyqxmd.com/read/28810568/mechanism-of-microrna-146a-mediated-il-6-stat3-signaling-in-lumbar-intervertebral-disc-degeneration
#1
Tao Zhou, Hao Lin, Ziao Cheng, Chaochao Ji, Chao Zhang, Jiwei Tian
The aim of the study was to investigate the mechanism of microRNA (miR)-146a-mediated activation of interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) in lumbar intervertebral disc degeneration. To obtain intervertebral tissue, we recruited 5 patients with lumbar intervertebral disc herniation (experimental group) and 5 patients with lumbar burst fracture (control group). Nucleus pulposus tissue was extracted by surgery and cultured. miR-146a empty vector, mimic, and inhibitor were transfected into the two groups of cells for 24 h and the levels of IL-6, type II collagen (Col II), aggrecan, STAT3, matrix metalloproteinase (MMP)-3, and a disintegrin and metalloproteinase with thrombospondin type I motifs (ADAMTS) were detected...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28799675/biomimetic-implants-for-pelvic-floor-repair
#2
REVIEW
Mahshid Vashaghian, Sebastianus J Zaat, Theodoor H Smit, Jan-Paul Roovers
BACKGROUND: Polypropylene implants are used for the reconstructive surgery of urogynaecological disorders like pelvic organ prolapse, but severe complications associated with their use have been reported. There is evidence that surface properties and a difference in mechanical stiffness between the implant and the host tissue contribute to these adverse events. Electrospinning is an innovative engineering alternative that provides a biomimetic microstructure for implants, resulting in a different mechano-biological performance...
August 11, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28795841/serous-carcinoma-mimicking-primary-urothelial-carcinoma-on-clinical-evaluation-and-pathology-a-potential-diagnostic-pitfall
#3
Leili Mirsadraei, Alexey Hodkoff, Karra Jones, Ahmed Shabaik, A Karim Kader, Cheryl C Saenz, Rodolfo Montironi, David E Tacha, Oluwole Fadare, Donna E Hansel
CONTEXT: - Serous carcinoma of the gynecologic tract often involves the external bladder wall and can occasionally mimic primary urothelial carcinoma of the bladder. OBJECTIVE: - To define the spectrum of morphologic and immunohistochemical features that characterize serous carcinoma involving the bladder wall and its distinction from urothelial carcinoma. DESIGN: - We reviewed all cases of serous carcinoma secondarily involving the bladder wall from the University of California San Diego and Polytechnic Institute for histopathologic and immunohistochemical features...
August 10, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28795385/hsa_circ_0045714-regulates-chondrocyte-proliferation-apoptosis-and-extracellular-matrix-synthesis-by-promoting-the-expression-of-mir-193b-target-gene-igf1r
#4
Bao-Feng Li, Ying Zhang, Jin Xiao, Fei Wang, Mei Li, Xiao-Ze Guo, Hui-Bin Xie, Hong Xia, Bei Chen
In recent years, some studies have been made on the effects of circular RNA (circRNA) in osteoarthritis (OA) and so on; however, its mechanisms remain to be further explored. Studies have shown that tumor necrosis factor-alpha can inhibit hsa_circ_0045714 expression in chondrocytes so as to upregulate miR-193b expression. Dual-luciferase reporter assay showed that insulin-like growth factor 1 receptor (IGF1R) is a key target gene of miR-193b. Hsa_circ_0045714 over-expression does not influence miR-193b expression, but can inhibit its transcriptional activity, thereby upregulating IGF1R expression...
August 9, 2017: Human Cell
https://www.readbyqxmd.com/read/28793225/multiscale-and-multiaxial-mechanics-of-vascular-smooth-muscle
#5
Sae-Ii Murtada, Jay D Humphrey, Gerhard A Holzapfel
Mathematical models can facilitate an integrative understanding of the complexity underlying biological structure and function, but they must be informed and validated by empirical data. Uniaxial contraction of an arterial ring is a well-used in vitro approach for studying characteristics of smooth muscle contractility even though this experimental arrangement does not mimic the in vivo vascular geometry or loading. In contrast, biaxial contraction of an inflated and axially extended excised vessel provides broader information, both passive and active, under more realistic conditions...
August 8, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28783434/highly-stereoselective-heterogeneous-diene-polymerization-by-co-mfu-4l-a-single-site-catalyst-prepared-by-cation-exchange
#6
Romain Dubey, Robert J Comito, Zhenwei Wu, Guanghui Zhang, Adam J Rieth, Christopher H Hendon, Jeffrey T Miller, Mircea Dinca
Molecular catalysts offer tremendous advantages for stereoselective polymerization because their activity and selectivity can be optimized and understood mechanistically using the familiar tools of organometallic chemistry. Yet, this exquisite control over selectivity comes at an operational price that is generally not justifiable for the large-scale manufacture of polyfolefins. In this report we identify Co-MFU-4l, prepared by cation exchange in a metal-organic framework, as a solid catalyst for the polymerization of 1,3-butadiene with high stereoselectivity (>99 % 1,4-cis)...
August 7, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28780604/mir-338-5p-promotes-glioma-cell-invasion-by-regulating-tshz3-and-mmp2
#7
Yanyan Li, Yulun Huang, Zhenyu Qi, Ting Sun, Youxin Zhou
This study was designed to examine differential expression of miR-338-5p in gliomas and the role of miR-338-5p in glioma cell invasion via its potential target gene TSHZ3 encoding Teashirt zinc finger homobox 3, predicted by bioinformatics, and matrix metallopeptidase 2 (MMP2), the key pro-invasive protease overexpressed in gliomas. Quantitative real-time reverse transcription PCR (qRT-PCR) and Spearman correlation analysis were used to determine differential expressions of miR-338-5p and TSHZ3 in astrocytic gliomas of different grades (n = 35) and glioblastoma cell lines (U87 and U251) in comparison to non-neoplastic brain (NNB) tissues (n = 6)...
August 5, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28777535/electrospun-nanofibers-new-concepts-materials-and-applications
#8
Jiajia Xue, Jingwei Xie, Wenying Liu, Younan Xia
Electrospinning is a simple and versatile technique that relies on the electrostatic repulsion between surface charges to continuously draw nanofibers from a viscoelastic fluid. It has been applied to successfully produce nanofibers, with diameters down to tens of nanometers, from a rich variety of materials, including polymers, ceramics, small molecules, and their combinations. In addition to solid nanofibers with a smooth surface, electrospinning has also been adapted to generate nanofibers with a number of secondary structures, including those characterized by a porous, hollow, or core-sheath structure...
August 4, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28766941/a-computational-investigation-of-small-molecule-engagement-of-hot-spots-at-protein-protein-interaction-interfaces
#9
David Xu, Yubing Si, Samy O Meroueh
The binding affinity of a protein-protein interaction is concentrated at amino acids known as hot spots. It has been suggested that small molecules disrupt protein-protein interactions by either (i) engaging receptor protein hot spots; or (ii) mimicking hot spots of the protein ligand. Yet, to date, no systematic studies have been done to explore how effectively existing small-molecule protein-protein interaction inhibitors mimic or engage hot spots at protein interfaces. Here, we employ explicit-solvent molecular dynamics simulations and end-point MM-GBSA free energy calculations to explore this question...
August 2, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28766848/microrna-30a-ameliorates-hepatic-fibrosis-by-inhibiting-beclin1-mediated-autophagy
#10
Jianliang Chen, Yue Yu, Shu Li, Yuting Liu, Shu Zhou, Shouji Cao, Jie Yin, Guoqiang Li
We explored the role of microRNA-30a (miR-30a) and the mechanism involved in hepatic fibrosis. MiR-30a overexpression was achieved by miR-30a mimics transfection in hepatic stellate cells (HSCs) (HSC-T6, LX-2), and miR-30a agomir (ago-miR-30a) treatment in mice. MiR-30a levels were measured using TaqMan miRNA assay system, and the localization of miR-30a was detected by fluorescence in situ hybridization (FISH). The interaction of miR-30a and Beclin1 was confirmed by dual-luciferase reporter assay. Autophagic flux was analysed using tandem mRFP-GFP-LC3 fluorescence microscopy, electron microscopy and Western blot of LC3-II/I ratio...
August 1, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28762369/variable-presentation-of-retrocaval-ureter-report-of-four-cases-and-review-of-literature
#11
Muhammed Ahmed, Abdullahi Alhassan, Muhammad Abubakar Sadiq, Ahmad Tijjani Lawal, Ahmad Bello, Hussaini Yusuf Maitama
Retrocaval ureter is a rare anomaly in which the ureter passes behind the inferior vena cava due to abnormal embryogenesis. Very few cases have been reported from Africa. Although the anomaly is congenital, patients become symptomatic in the third or fourth decade of life. We reviewed the records of four patients with the diagnosis of retrocaval ureter and managed in our centre between January 2010 and December 2016. Three patients presented with recurrent colicky right flank pain while one was asymptomatic...
April 2017: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/28757936/three-point-bending-test-simulation-on-implant-fpds-with-a-bio-faithful-model
#12
V Vertucci, M Montani, C Arcuri, P Cardelli
AIM OF THE STUDY: It is well known by previous important studies that mandible flexes during different jaw movements. According to this assumption it is very important to know how implant supported fixed partial dentures could restrict mandibular movements and, could lead to excess strain accumulation that could modify the resolution of implant treatment. The aim of our project is to create a bio-faithful model able to recreate mandibular movements, during three point bending test methods of (FIXED -PARTIAL -DENTURES) FPDs, to avoid a not flexible metal base, where models' properties doesn't allow to obtain a bio-faithful simulation during testing phases...
January 2017: Oral & Implantology
https://www.readbyqxmd.com/read/28756737/mimicking-the-biochemical-and-mechanical-extracellular-environment-of-the-endochondral-ossification-process-to-enhance-the-in-vitro-mineralization-potential-of-human-mscs
#13
Fiona E Freeman, Jessica Schiavi, Meadhbh A Brennan, Peter Owens, Pierre Layrolle, Laoise McNamara
Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis...
July 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28746083/novel-cardiac-coordinate-modeling-system-for-three-dimensional-quantification-of-inflow-cannula-malposition-of-heartmate-ii-lvads
#14
Brian Lima, Onur Dur, Joyce Chuang, Themistokles Chamogeorgakis, David J Farrar, Kartik S Sundareswaran, Joost Felius, Susan M Joseph, Shelley A Hall, Gonzalo V Gonzalez-Stawinski
Optimal function of left ventricular assist devices (LVADs) depends on proper alignment of the inflow cannula (IC). Quantitative guidelines for IC angulation are lacking because of variation in cardiac geometry and difficulty in analyzing three-dimensional (3D) cannula orientation relative to the left ventricle (LV). Based on contrast-enhanced computed tomography images from five normal and five clinically malpositioned IC cases in patients with HeartMate II LVADs, we developed a method for 3D quantification of IC malpositioning...
July 25, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28746067/perturbing-nr2b-psd-95-interaction-relieves-neuropathic-pain-by-inactivating-camkii-creb-signaling
#15
Fangxia Xu, Xin Zhao, Lin Liu, Jia Song, Yingjun Zhu, Shuaishuai Chu, Xueming Shao, Xiuxiu Li, Zhengliang Ma, Xiaoping Gu
Neuropathic pain is characterized by central sensitization. The interaction between N-methyl-D-aspartate receptors (NMDARs) and postsynaptic density protein-95 (PSD-95) plays a major role in central sensitization. Here, we aimed to investigate the analgesic effect of disruption of the interaction between NMDAR and PSD-95. Chronic dorsal root ganglia compression model rats were used to mimic sciatica. Thermal hyperalgesia and mechanical allodynia were evaluated. The expression of spinal phospho-NR2B, PSD-95, calcium/calmodulin-dependent protein kinase II (CaMKII), and cAMP response element binding protein (CREB) was measured using western blotting...
September 6, 2017: Neuroreport
https://www.readbyqxmd.com/read/28742157/angiotensin-ii-system-in-the-nucleus-tractus-solitarii-contributes-to-autonomic-dysreflexia-in-rats-with-spinal-cord-injury
#16
Kai Wang, Shaoxia Duan, Xueping Wen, Weizhong Wang, Shangping Fang, Dunyi Qi, Xiang Huan, Liwei Wang, Zhenzhou He
BACKGROUND: Autonomic dysreflexia (AD) is a potentially life-threating complication after spinal cord injury (SCI), characterized by episodic hypertension induced by colon or bladder distension. The objective of this study was to determine the role of impaired baroreflex regulation by the nucleus tractus solitarii(NTS) in the occurrence of AD in a rat model. METHODS: T4 spinal cord transection animal model was used in this study, which included 40 Male rats Colorectal distension (CD) was performed to assess AD and compare the changes of BP, HR, and BRS, six weeks after operation...
2017: PloS One
https://www.readbyqxmd.com/read/28739533/effect-of-bisphenol-a-bpa-on-insulin-signal-transduction-and-glut4-translocation-in-gastrocnemius-muscle-of-adult-male-albino-rat
#17
Vigneswari Mullainadhan, Mangala Priya Viswanathan, Balasubramanian Karundevi
Environmental estrogens bind to estrogen receptors, mimic estrogenic actions, and have adverse effects on human health like Bisphenol - A (BPA) which is used as a monomer in the production of polycarbonate plastics (PC) and epoxy resins which are used in variety of canned foods. Skeletal muscle plays an essential role in maintaining systemic glucose metabolism. In the present study, we investigated the possible effects of BPA on insulin signalling molecules and GLUT4 translocation in the gastrocnemius muscle of adult male rat...
July 21, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28739306/the-in-vitro-and-in-vivo-effects-of-microrna-133a-on-intervertebral-disc-destruction-by-targeting-mmp9-in-spinal-tuberculosis
#18
Xin-Wen Wang, Ji-Jun Liu, Qi-Ning Wu, Shu-Fang Wu, Ding-Jun Hao
AIMS: We aim to investigate the role of microRNA-133a (miR-133a) in intervertebral disc destruction by targeting MMP9 in spinal tuberculosis (TB). MAIN METHODS: Rabbit models with spinal TB were established and assigned to the blank, miR-133a mimic, miR-133a inhibitor and negative control (NC) groups. Primary notochordal cells were extracted and separately transfected with miR-133a mimics, miR-133a inhibitor, miR-nonsense sequence control (NC), si-NC and si-MMP9...
July 21, 2017: Life Sciences
https://www.readbyqxmd.com/read/28736342/tfos-dews-ii-diagnostic-methodology-report
#19
REVIEW
James S Wolffsohn, Reiko Arita, Robin Chalmers, Ali Djalilian, Murat Dogru, Kathy Dumbleton, Preeya Gupta, Paul Karpecki, Sihem Lazreg, Heiko Pult, Benjamin D Sullivan, Alan Tomlinson, Louis Tong, Edoardo Villani, Kyung Chul Yoon, Lyndon Jones, Jennifer Craig
The role of the Tear Film and Ocular Surface Society (TFOS) Dry Eye Workshop (DEWS) II Diagnostic Methodology Subcommittee was 1) to identify tests used to diagnose and monitor dry eye disease (DED), 2) to identify those most appropriate to fulfil the definition of DED and its sub-classifications, 3) to propose the most appropriate order and technique to conduct these tests in a clinical setting, and 4) to provide a differential diagnosis for DED and distinguish conditions where it is a comorbidity. Symptom screening with the DEQ-5 or OSDI confirms that a patient might have DED and triggers the diagnostic tests of (ideally non-invasive) breakup time, osmolarity and ocular surface staining with fluorescein and lissamine green (observing the cornea, conjunctiva and eyelid margin)...
May 4, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28736221/scaffold-curvature-mediated-novel-biomineralization-process-originates-a-continuous-soft-tissue-to-bone-interface
#20
Michael Paris, Andreas Götz, Inga Hettrich, Cécile M Bidan, John W C Dunlop, Hajar Razi, Ivo Zizak, Dietmar W Hutmacher, Peter Fratzl, Georg N Duda, Wolfgang Wagermaier, Amaia Cipitria
A myriad of shapes are found in biological tissues, often naturally evolved to fulfill a particular function. In the field of tissue engineering, substrate geometry influences cell behavior and tissue formation in-vitro, yet little is known how this translates to an in-vivo scenario. Here we investigate scaffold curvature-induced tissue growth, without additional growth factors or cells, in an ovine animal model. We show that soft tissue formation follows a curvature-driven tissue growth model. The highly organized endogenous soft matrix, potentially under mechanical strain, leads to a non-standard form of biomineralization, whereby the pre-existing organic matrix is mineralized without collagen remodeling and without an intermediate cartilage ossification phase...
July 20, 2017: Acta Biomaterialia
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