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https://www.readbyqxmd.com/read/28214451/a-novel-lc-ms-ms-method-for-the-simultaneous-quantification-of-topiramate-and-its-main-metabolites-in-human-plasma
#1
Daniela Milosheska, Robert Roškar
The aim of the present report was to develop and validate simple, sensitive and reliable LC-MS/MS method for quantification of topiramate (TPM) and its main metabolites: 2,3-desisopropylidene TPM, 4,5-desisopropylidene TPM, 10-OH TPM and 9-OH TPM in human plasma samples. The most abundant metabolite 2,3-desisopropylidene TPM was isolated from patients urine, characterized and afterwards used as an authentic standard for method development and validation. Sample preparation method employs 100μL of plasma sample and liquid-liquid extraction with a mixture of ethyl acetate and diethyl ether as extraction solvent...
February 3, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28214448/effect-of-fluoride-toothpaste-with-nano-sized-trimetaphosphate-on-enamel-demineralization-an-in-vitro-study
#2
Marcelle Danelon, Juliano Pelim Pessan, Francisco Nunes Souza-Neto, Emerson Rodrigues de Camargo, Alberto Carlos Botazzo Delbem
OBJECTIVE: This study evaluated the effect of toothpastes containing 1100ppm F associated or not with micrometric or nano-sized sodium trimetaphosphate (TMP) on enamel demineralization in vitro, using a pH cycling model. DESIGN: Bovine enamel blocks (4mm×4mm, n=96) were randomly allocated into eight groups (n=12), according to the test toothpastes: Placebo (without fluoride or TMP); 1100ppm F (1100F); 1100F plus micrometric TMP at concentrations of 1%, 3% or 6%; and 1100F plus nanosized TMP at 1%, 3% or 6%...
February 10, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28214399/magnetite-platinum-nanoparticles-modified-glassy-carbon-electrode-as-electrochemical-detector-for-nitrophenol-isomers
#3
Giles G Gerent, Almir Spinelli
A glassy carbon electrode was modified with magnetite and platinum nanoparticles stabilized with 3-n-propyl-4-picoline silsesquioxane chloride. This chemically-modified electrode is proposed for the first time for the individual or simultaneous electrochemical detection of nitrophenol isomers. Nanoparticles act as catalysts and also increase the surface area. The polymer stabilizes the particles and provides the electrochemical separation of isomers. Under optimized conditions, the reduction peak currents, obtained by differential-pulse voltammetry, of 2-, 3-, and 4-nitrophenol increased linearly with increases in their concentration in the range of 0...
February 9, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28214385/temporal-changes-in-physiology-and-haematology-in-response-to-high-and-micro-doses-of-recombinant-human-erythropoietin
#4
Brad Clark, Sarah M Woolford, Annette Eastwood, Ken Sharpe, Peter G Barnes, Christopher J Gore
There is evidence to suggest athletes have adopted recombinant human erythropoietin (rHuEPO) dosing regimens that diminish the likelihood they will be caught by direct detection techniques. However, the temporal response in physiology, performance and athlete biological passport (ABP) parameters to such regimens is not clearly understood. Participants were assigned to a high dose only group (HIGH, n = 8, six rHuEPO doses of 250 IU/kg over two weeks), a combined high micro-dose group (COMB, n = 8, high dose plus nine rHuEPO micro-doses over a further three weeks), or one of two placebo control groups who received saline in the same pattern as the HIGH (HIGH-PLACEBO, n = 4) or COMB (COMB-PLACEBO, n = 4) groups...
February 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28214384/placental-pathology-in-hiv-infection-at-term-a-comparison-with-hiv-uninfected-women
#5
Emma Kalk, Pawel Schubert, Julie A Bettinger, Mark F Cotton, Monika Esser, Amy Slogrove, Colleen A Wright
OBJECTIVES: To describe and correlate placental characteristics from pregnancies in HIV-infected and HIV-negative women with maternal and infant clinical and immunological data. METHODS: Prospective descriptive study of placentas from term, uncomplicated vaginal births in a cohort of HIV-infected (n=120) and HIV-negative (n=103) women in Cape Town, South Africa. Microscopic and macroscopic features were used to determine pathological cluster diagnoses. The majority of HIV-infected women received some form of drug treatment for the prevention of vertical transmission of HIV...
February 18, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28214376/sensitive-quantification-of-coixol-a-potent-insulin-secretagogue-in-scoparia-dulcis-extract-using-high-performance-liquid-chromatography-combined-with-tandem-mass-spectrometry-and-uv-detection
#6
Arslan Ali, Faraz Ul Haq, Qamar Ul Arfeen, Khaga Raj Sharma, Achyut Adhikari, Syed Ghulam Musharraf
Diabetes is a major global health problem which requires new studies for its prevention and control. Scoparia dulcis, an herbal product, is widely used for treatment of diabetes. Recent studies demonstrate coixol as a potent and non-toxic insulin secretagogue from S. dulcis. This study focuses on developing two quantitative methods of coixol in Scoparia dulcis methanol-based extracts. Quantification of coixol was performed using high performance liquid chromatography-tandem mass spectrometry (method 1) and high performance liquid chromatography-ultraviolet detection (method 2) with limit of detection of 0...
February 18, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28214372/canonical-and-cellular-pathways-timing-gamete-release-in-acropora-digitifera-okinawa-japan
#7
Y Rosenberg, T Doniger, S Harii, F Sinniger, O Levy
Natural light cycles are important for synchronizing behavioral and physiological rhythms over varying time-periods in both plants and animals. An endogenous clock, regulated by positive and negative elements, interacting in feedback loops, control these rhythms. Many corals exhibit diel cycles of polyp expansion and contraction entrained by solar light patterns and monthly cycles of spawning or planulation that correspond to nocturnal lunar light cycles. However, despite considerable interest in studies of coral reproduction, there is currently not enough molecular information about the cellular pathways involved with synchronizing spawning/planulation in broadcast spawners and brooders...
February 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28214369/diagnostic-accuracy-of-history-physical-exam-laboratory-tests-and-point-of-care-ultrasound-for-pediatric-acute-appendicitis-in-the-emergency-department-a-systematic-review-and-meta-analysis
#8
Roshanak Benabbas, Mark Hanna, Jay Shah, Richard Sinert
BACKGROUND: Acute appendicitis (AA) is the most common surgical emergency in children. Accurate and timely diagnosis is crucial but challenging due to atypical presentations and the inherent difficulty of obtaining a reliable history and physical examination in younger children. OBJECTIVES: To determine the utility of history, physical exam, laboratory tests, Pediatric Appendicitis Score (PAS) and Emergency Department-Point-of-Care Ultrasound (ED-POCUS) in the diagnosis of AA in ED pediatric patients...
February 18, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28214368/evaluation-of-deformable-image-registration-between-external-beam-radiotherapy-and-hdr-brachytherapy-for-cervical-cancer-with-a-3d-printed-deformable-pelvis-phantom
#9
Noriyuki Kadoya, Yuya Miyasaka, Yujiro Nakajima, Yoshihiro Kuroda, Kengo Ito, Mizuki Chiba, Kiyokazu Sato, Suguru Dobashi, Takaya Yamamoto, Noriyoshi Takahashi, Masaki Kubozono, Ken Takeda, Keiichi Jingu
PURPOSE: In this study, we developed a 3D-printed deformable pelvis phantom for evaluating spatial DIR accuracy. We then evaluated the spatial DIR accuracies of various DIR settings for cervical cancer. METHODS: A deformable female pelvis phantom was created based on patient CT data using 3D printing. To create the deformable uterus phantom, we first 3D printed both a model of uterus and a model of the internal cavities of the vagina and uterus. We then made a mold using the 3D printed uterus phantom...
February 18, 2017: Medical Physics
https://www.readbyqxmd.com/read/28214367/population-genetic-structure-and-adaptation-of-malaria-parasites-on-the-edge-of-endemic-distribution
#10
Craig W Duffy, Hampate Ba, Samuel Assefa, Ambroise D Ahouidi, Yacine B Deh, Abderahmane Tandia, Freja C M Kirsebom, Dominic P Kwiatkowski, David J Conway
To determine whether the major human malaria parasite Plasmodium falciparum exhibits fragmented population structure or local adaptation at the northern limit of its African distribution where the dry Sahel zone meets the Sahara, samples were collected from diverse locations within Mauritania over a range of ~ 1000 kilometres. Microsatellite genotypes were obtained for 203 clinical infection samples from eight locations, and Illumina paired-end sequences were obtained to yield high coverage genome-wide single nucleotide polymorphism (SNP) data for 65 clinical infection samples from four locations...
February 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28214364/in-situ-analysis-of-bacillus-licheniformis-biofilms-amyloid-like-polymers-and-edna-are-involved-in-the-adherence-and-aggregation-of-the-extracellular-matrix
#11
I Randrianjatovo-Gbalou, P Rouquette, D Lefebvre, E Girbal-Neuhauser, C-E Marcato-Romain
AIMS: This study attempts to determine which exopolymeric substances are involved in the adherence and aggregation of a Bacillus licheniformis biofilm. METHODS AND RESULTS: The involvement of extracellular proteins and eDNA were particularly investigated using DNase and proteinase K treatment. The permeability of the biofilms increased 5-fold after DNase I-treatment. Quantification of the matrix components showed that, whatever the enzyme tested, some eDNA and amyloid-like polymers were removed simultaneously...
February 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28214362/is-there-an-association-between-verbal-school-bullying-and-possible-sleep-bruxism-in-adolescents
#12
Junia Maria Serra-Negra, Isabela Almeida Pordeus, Patricia Corrêa-Faria, Livia Bonfim Fulgêncio, Saul Martins Paiva, Daniele Manfredini
The aim of this study was to determine the association between verbal school bullying and possible sleep bruxism (SB) in adolescents. A case-control study was carried out at the population level by recruiting 13- to 15-year old participants among the attendants of schools of Itabira, Brazil. The case group was composed of 103 adolescents with possible SB (i.e., self- or parental-reported), while the control group included 206 adolescents without possible SB. All participants answered a questionnaire on the occurrence of their involvement in verbal school bullying episodes, based on the National School of Health Research (PeNSE) as well as an evaluation of their economic class according to the criteria of the Brazilian Association of Research Companies...
February 18, 2017: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/28214361/identification-of-arabidopsis-genic-and-non-genic-promoters-by-paired-end-sequencing-of-tss-tags
#13
Mutsutomo Tokizawa, Kazutaka Kusunoki, Hiroyuki Koyama, Atsushi Kurotani, Tetsuya Sakurai, Yutaka Suzuki, Tomoaki Sakamoto, Tetsuya Kurata, Yoshiharu Y Yamamoto
Information about transcription start sites (TSSs) provides foundational data for analyses of promoter architecture. In this report, we used paired- and single-end deep sequencing to analyze Arabidopsis TSS tags from several libraries prepared from roots, shoots, flowers, and etiolated seedlings. The clustering of approximately 33 million mapped TSS tags led to the identification of 324,461 promoters that covered 79.7% (21,672/27,206) of protein-coding genes in the Arabidopsis genome. In addition, we identified intragenic, antisense, and Orphan promoters that were not associated with any gene models...
February 18, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28214348/organo-protective-and-antioxidant-properties-of-leaf-extracts-of-syzygium-guineense-var-macrocarpum-against-ferric-nitriloacetate-induced-stress-of-wistar-rats
#14
Francine Tankeu Nzufo, Constant Anatole Pieme, Jacques Romain Njimou, Prosper Cabral Biapa Nya, Bruno Moukette Moukette, Bravi Marco, Chianese Angelo, Ngogang Jeanne Yonkeu
Background The present study focused on the antioxidant, phenolic profile and free radical scavenging-mediated protective effect of leaves extracts of Syzygium guineense var. macrocarpum against ferric nitriloacetate-induced stress in the liver, heart, kidney and brain tissues of Wistar rats homogenates. Methods Spectrophotometric standardized methods were used to determine the free radical scavenging potential, antioxidant and protective properties of plant extracts on rat homogenates. Results All the extracts showed a concentration-dependent free radical quenching potential, and the ability to protect all the tested organs by inhibiting the lipid peroxidation and potentiating or restoring the activity of enzymatic and non enzymatic markers...
February 16, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28214337/palmitate-induces-mitochondrial-superoxide-generation-and-activates-ampk-in-podocytes
#15
Eugene Lee, Jin Choi, Hyun Soon Lee
Studies have shown that high levels of serum free fatty acids (FFAs) are associated with lipotoxicity and type 2 diabetes. Palmitic acid (PA) is the predominant circulating saturated FFA, yet its role in the pathogenesis of diabetic nephropathy (DN) is not clear. Recently, one study suggested that mitochondrial superoxide production is related to AMP-activated protein kinase (AMPK) activity in diabetic mice kidneys. To elucidate the link between PA and oxidative stress and AMPK activity in DN, we compared the cultured murine podocytes exposed to PA and oleic acid (OA)...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28214331/pomc-maintains-tumor-initiating-properties-of-tumor-tissue-derived-long-term-cultured-breast-cancer-stem-cells
#16
Xiaoti Lin, Weiyu Chen, Fengqin Wei, Binhua P Zhou, Mien-Chie Hung, Xiaoming Xie
The identification and understanding of the molecular network of cancer stem cells (CSCs) have had a profound impact on our view of carcinogenesis and treatment strategy. Unfortunately, a major problem is that serial passages of CSCs from clinical solid tumor specimens currently are not available in any lab, and thus, reported data are difficult to confirm and intensively interrogated. Here, we have generated two tumor tissue-derived breast CSC (BCSC) lines that showed prolonged maintenance over 20 serial passages in vitro, while retaining their tumor-initiating biological properties...
February 18, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28214329/-p-clphse-2-reduces-hepatotoxicity-induced-by-monosodium-glutamate-by-improving-mitochondrial-function-in-rats
#17
Caroline B Quines, Pietro M Chagas, Diane Hartmann, Nélson R Carvalho, Félix A Soares, Cristina W Nogueira
It is has been demonstrated that mitochondrial dysfunction, oxidative stress and chronic inflammatory process are associated with progress of morbid obesity in human patients. For this reason, the searching for safe and effective antiobesity drugs has been the subject of intense research. In this context, the organic selenium compounds have attracted much attention due to their pharmacological properties, such as antihyperglycemic, antioxidant and anti-inflammatory. The aim of this study was to evaluate the hepatoprotective action of p-chloro-diphenyl diselenide (p-ClPhSe)2 , an organic selenium compound, in a model of obesity induced by monosodium glutamate (MSG) administration in rats...
February 18, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28214326/treatment-preferences-for-biologicals-in-psoriasis-experienced-patients-appreciate-sustainability
#18
Christian Kromer, Wiebke K Peitsch, Raphael Herr, Astrid Schmieder, Diana Sonntag, Marthe-Lisa Schaarschmidt
BACKGROUND AND OBJECTIVES: Treatment satisfaction can be improved by integrating patients' preferences into shared decision-making. We recently investigated patients' preferences for attributes of biologicals, and showed high preferences for safety and efficacy. The objective of the present study was to assess the impact of treatment experience on these preferences. PATIENTS AND METHODS: Preferences for outcome (probability of 50 % and 90 % improvement, time until response, sustainability of success, probability of mild and severe adverse events, probability of ACR 20 response) and process attributes (treatment location, frequency, duration, and delivery method) were analyzed in 200 participants with moderate-to-severe psoriasis using conjoint analysis...
February 2017: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/28214293/ultra-high-frequency-ultrasound-of-fascicles-in-the-median-nerve-at-the-wrist
#19
Michael S Cartwright, Vanessa Baute, James B Caress, Francis O Walker
INTRODUCTION: An ultra high-frequency (70 MHz) ultrasound device has recently been approved for human use. This study was conducted to determine if this device facilitates counting of fascicles within the median nerve at the wrist. METHODS: Twenty healthy volunteers underwent imaging of the median nerve at the wrist bilaterally. The number of fascicles in each nerve was counted by two independent raters. RESULTS: The mean fascicle number was 22...
February 18, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28214292/prescription-profile-of-pyridostigmine-use-in-a-population-of-patients-with-myasthenia-gravis
#20
Jorge Enrique Machado-Alba, Luis Felipe Calvo-Torres, Andrés Gaviria-Mendoza, César Augusto Mejía-Vélez
INTRODUCTION: We determined the pyridostigmine prescription pattern in a population of patients with myasthenia gravis. METHODS: A descriptive cross-sectional study was done using a prescription database of 3.5 million individuals from which patients diagnosed with myasthenia gravis (MG) and for whom pyridostigmine was prescribed. RESULTS: A total of 306 outpatients with MG were found, and 258 were receiving pyridostigmine, mean age, 53.0±18...
February 18, 2017: Muscle & Nerve
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