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https://www.readbyqxmd.com/read/28549370/assessment-of-the-influence-of-key-abiotic-factors-on-the-alternative-microcystin-degradation-pathway-s-mlr-a-detailed-comparison-with-the-mlr-route-mlr
#1
Jesús Morón-López, Lucía Nieto-Reyes, Rehab El-Shehawy
Cyanobacterial proliferation and toxin production in water bodies around the world have led to global concern about the control of these issues. Indigenous bacteria have been shown to degrade the cyanotoxin microcystin (MC) in natural environments. The mlr cluster has been widely used as a marker for microcystin biodegradation; however, recent studies have shown that alternative pathway(s) also contribute to the natural removal of MCs in the ecosystem. The main objective of this study is to provide initial insights concerning how key abiotic factors affect the rate of MC biodegradation via alternative pathway(s) and to provide a detailed comparison with the mlr(+) pathway...
May 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28549361/-leukocyte-esterase-as-a-diagnostic-tool-for-an-infectious-disease-of-the-knee
#2
S U Ceja-Picazo, S Fuentes-Figueroa, A H Rivera-Villa, A R Hernández-Salgado, R Torres-González, A M Pérez, J A Hernández-García, J M Pérez-Atanasio
BACKGROUND: Articular infection is an orthopedic challenge due to its difficult diagnosis and devastating results. Various diagnostic studies exist: culture, ESR, CRP, count of leukocytes, among others, but none is specific, they all take more than 30 minutes to complete, and require complex infrastructure. In this study, we determine the sensitivity and specificity of the leukocyte esterase for detection of an infectious process joint in Mexican population. MATERIAL AND METHODS: During November 2015 to April 2016, we obtained synovial fluid from two groups of patients: one with a diagnosis of synovial joint infection with or without implant, and the control group, without infection but with degenerative pathology of the knee...
November 2016: Acta Ortopédica Mexicana
https://www.readbyqxmd.com/read/28549354/the-facespan-the-perceptual-span-for-face-recognition
#3
Michael Papinutto, Junpeng Lao, Meike Ramon, Roberto Caldara, Sébastien Miellet
In reading, the perceptual span is a well-established concept that refers to the amount of information that can be read in a single fixation. Surprisingly, despite extensive empirical interest in determining the perceptual strategies deployed to process faces and an ongoing debate regarding the factors or mechanism(s) underlying efficient face processing, the perceptual span for faces-the Facespan-remains undetermined. To address this issue, we applied the gaze-contingent Spotlight technique implemented in an old-new face recognition paradigm...
May 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28549353/crowding-visual-awareness-and-their-respective-neural-loci
#4
Kilho Shin, Susana T L Chung, Bosco S Tjan
In peripheral vision, object identification can be impeded when a target object is flanked by other objects. This phenomenon of crowding has been attributed to basic processes associated with image encoding by the visual system, but the neural origin of crowding is not known. Determining whether crowding depends on subjective awareness of the flankers can provide information on the neural origin of crowding. However, recent studies that manipulated flanker awareness have yielded conflicting results. In the current study, we suppressed flanker awareness with two methods: interocular suppression (IOS) and adaptation-induced blindness (AIB)...
May 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28549340/a-critical-assessment-of-the-in-vitro-measurement-of-cortical-bone-stiffness-with-ultrasound
#5
L Peralta, X Cai, P Laugier, Q Grimal
Elasticity assessment based on bulk wave velocity (BWV) measurements is the most popular technique to characterize the anisotropic stiffness tensor in cortical bone. Typically, a cuboid bone specimen is cut with its sides along the different anatomical directions. Then, the velocity of shear and longitudinal waves propagating along different directions are assessed, from which stiffness coefficients are calculated. Despite the importance of obtaining accurate elasticity values for bone research, there is no generally accepted protocol to measure BWV and the precision of the technique has been seldom investigated...
May 19, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28549333/endoplasmic-reticulum-er-stress-and-camp-pka-pathway-mediated-zn-induced-hepatic-lipolysis
#6
Yu-Feng Song, Christer Hogstrand, Chuan-Chuan Wei, Kun Wu, Ya-Xiong Pan, Zhi Luo
The present study was performed to determine the effect of Zn exposure influencing endoplasmic reticulum (ER) stress, explore the underlying molecular mechanism of Zn-induced hepatic lipolysis in a fish species of significance for aquaculture, yellow catfish Pelteobagrus fulvidraco. We found that waterborne Zn exposure evoked ER stress and unfolded protein response (UPR), and activated cAMP/PKA pathway, and up-regulated hepatic lipolysis. The increase in ER stress and lipolysis were associated with activation of cAMP/PKA signaling pathway...
May 23, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28549321/studies-on-free-radical-scavenging-cancer-cell-antiproliferation-and-calf-thymus-dna-interaction-of-schiff-bases
#7
Cleiton M da Silva, Marina M Silva, Fabiano S Reis, Ana Lúcia T G Ruiz, João E de Carvalho, Josué C C Santos, Isis M Figueiredo, Rosemeire B Alves, Luzia V Modolo, Ângelo de Fátima
Thirty-nine Schiff bases were synthesized by performing microwave-assisted condensation of the corresponding aldehydes and aromatic amines. Their reactive nitrogen species (RNS) scavenging activity and inhibitory effects against cancer cell growth were then subsequently investigated. Additionally, the interaction between the calf thymus DNA (ctDNA) and selected Schiff bases was evaluated using fluorescence spectroscopy, and their binding parameters were determined. The yields of the various compounds ranged from moderate to excellent (43-99%) after only a 2-min reaction...
May 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28549308/climate-and-the-eye-case-crossover-analysis-of-retinal-detachment-after-exposure-to-ambient-heat
#8
Nathalie Auger, Marc-André Rhéaume, Marianne Bilodeau-Bertrand, Tina Tang, Tom Kosatsky
BACKGROUND: Retinal detachment is an important cause of visual loss, but the association with outdoor heat exposure has not been studied. Our objective was to determine the relationship between acute exposure to high outdoor temperature and risk of retinal detachment. MATERIALS AND METHODS: We analysed 14,302 individuals with inpatient procedures for retinal detachment from April through September between 2006 and 2013 in the province of Quebec, Canada. Using a time-stratified case-crossover study design, we examined the association of retinal detachment with outdoor summer temperature the preceding week...
May 23, 2017: Environmental Research
https://www.readbyqxmd.com/read/28549305/adsorption-of-steroid-micropollutants-on-polymer-based-spherical-activated-carbon-pbsac
#9
Matteo Tagliavini, Fabio Engel, Peter Georg Weidler, Torsten Scherer, Andrea Iris Schäfer
Removal and interaction mechanisms of four different steroid micropollutants, estrone (E1), estradiol (E2), progesterone (P) and testosterone (T) were determined for different types of polymer-based spherical activated carbon (PBSAC). Higher than 90% removal and significantly faster kinetics compared to conventional granular activated carbon (GAC) were observed, while performance was comparable with powdered activated carbon (PAC). No influence of pH in the range 2-12 was determined, while the presence of humic acid (HA) reduced both the removal and the kinetic by up to 20%...
April 4, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28549299/the-effect-of-feed-supplementation-with-effective-microorganisms-em-on-pro-and-anti-inflammatory-cytokine-concentrations-in-pigs
#10
Ewa Laskowska, Łukasz Jarosz, Zbigniew Grądzki
Effective microorganisms (EM) are used in numerous fields associated with agriculture. The beneficial effects of EM on the general health of pigs and on production parameters are also determined by the influence of these microbes on immunity. The aim of the study was to assess the effect of feed supplementation with EM on the concentration of pro- and anti-inflammatory cytokines in the serum and in a culture of PBMCs with and without ConA stimulation in pigs. ELISA kits were used to determine the cytokine levels in the porcine serum and the PBMC culture supernatants...
April 14, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28549294/chiral-recognition-of-naproxen-enantiomers-based-on-fluorescence-quenching-of-bovine-serum-albumin-stabilized-gold-nanoclusters
#11
Marzieh Jafari, Javad Tashkhourian, Ghodratollah Absalan
A simple, fast and green method for chiral recognition of S- and R-naproxen has been introduced. The method was based on quenching of the fluorescence intensity of bovine serum albumin-stabilized gold nanoclusters in the presence of naproxen enantiomers. The quenching intensity in the presence of S-naproxen was higher than R-naproxen when phosphate buffer solution at pH7.0 was used. The chiral recognition occurred due to steric effect between bovine serum albumin conformation and naproxen enantiomers. Two linear determination range were established as 7...
May 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28549293/automated-recognition-of-the-pericardium-contour-on-processed-ct-images-using-genetic-algorithms
#12
É O Rodrigues, L O Rodrigues, L S N Oliveira, A Conci, P Liatsis
This work proposes the use of Genetic Algorithms (GA) in tracing and recognizing the pericardium contour of the human heart using Computed Tomography (CT) images. We assume that each slice of the pericardium can be modelled by an ellipse, the parameters of which need to be optimally determined. An optimal ellipse would be one that closely follows the pericardium contour and, consequently, separates appropriately the epicardial and mediastinal fats of the human heart. Tracing and automatically identifying the pericardium contour aids in medical diagnosis...
May 17, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28549288/ecological-effects-of-soil-properties-and-metal-concentrations-on-the-composition-and-diversity-of-microbial-communities-associated-with-land-use-patterns-in-an-electronic-waste-recycling-region
#13
Wencheng Wu, Changxun Dong, Jiahui Wu, Xiaowen Liu, Yingxin Wu, Xianbin Chen, Shixiao Yu
Soil microbes play vital roles in ecosystem functions, and soil microbial communities may be strongly structured by land use patterns associated with electronic waste (e-waste) recycling activities, which can increase the heavy metal concentration in soils. In this study, a suite of soils from five land use types (paddy field, vegetable field, dry field, forest field, and e-waste recycling site) were collected in Longtang Town, Guangdong Province, South China. Soil physicochemical properties and heavy metal concentrations were measured, and the indigenous microbial assemblages were profiled using 16S rRNA high-throughput sequencing and clone library analyses...
May 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28549285/environmental-response-to-long-term-mariculture-activities-in-the-weihai-coastal-area-china
#14
Hongmei Li, Xiaomin Li, Qiang Li, Ying Liu, Jide Song, Yongyu Zhang
The environmental impacts of rapid expansion of mariculture have garnered worldwide attention. China is currently one of the largest countries to engage in this practice. In this study, a representative mariculture zone, the Weihai coastal area in China, was explored to determine the temporal variations in regional nutrients, N/P ratio, dissolved oxygen (DO), pH, chlorophyll a (Chl-a), and cellular abundance of diatoms and dinoflagellates in response to the rapid growth in mariculture activities between 2006 and 2014...
May 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28549276/different-seizure-onset-patterns-in-mesiotemporal-lobe-epilepsy-have-a-distinct-interictal-signature
#15
Birgit Frauscher, Nicolás von Ellenrieder, François Dubeau, Jean Gotman
OBJECTIVE: Experimental research demonstrated that distinct underlying mechanisms go along with different seizure-onset patterns on EEG. These different mechanisms may reflect different tissue abnormalities which, we hypothesize, could also be reflected in morphological differences in the interictal epileptic and background EEG activity. METHODS: We searched our database of intracranial EEG recordings for mesiotemporal lobe epilepsy patients with either predominant low-voltage fast activity (LVF) or periodic spiking (PS)...
May 9, 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/28549271/the-association-between-the-early-motor-repertoire-and-language-development-in-term-children-born-after-normal-pregnancy
#16
Sahar Salavati, Christa Einspieler, Giulia Vagelli, Dajie Zhang, Jasmin Pansy, Johannes G M Burgerhof, Peter B Marschik, Arend F Bos
BACKGROUND: The assessment of the early motor repertoire is a widely used method for assessing the infant's neurological status. AIM: To determine the association between the early motor repertoire and language development. STUDY DESIGN: Prospective cohort study. SUBJECTS: 22 term children born after normal pregnancy; video recorded for the assessment of the early motor repertoire including their motor optimality score (MOS), according to Prechtl, at 3 and 5months post term...
May 23, 2017: Early Human Development
https://www.readbyqxmd.com/read/28549266/self-cascade-reaction-catalyzed-by-cuo-nanoparticle-based-dual-functional-enzyme-mimics
#17
Ai-Ling Hu, Hao-Hua Deng, Xiao-Qing Zheng, Yan-Yu Wu, Xiu-Ling Lin, Ai-Lin Liu, Xing-Hua Xia, Hua-Ping Peng, Wei Chen, Guo-Lin Hong
It is desirable but challenging to assemble various biomimetic properties into a functional catalytic cascade system. In this work, cupric oxide nanoparticles were investigated as oxidase mimics for the aerobic oxidation of cysteine to cystine with the generation of hydrogen peroxide. Coupling this property with the peroxidase-like activity of CuO nanoparticles, we constructed a self-organized cascade reaction system based on a single-component nanozyme, which includes the oxidation of cysteine to yield cystine and hydrogen peroxide and the hydrogen peroxide-mediated oxidation of terephthalic acid to produce a fluorescence change...
May 19, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28549265/facile-screening-of-potential-xenoestrogens-by-an-estrogen-receptor-based-reusable-optical-biosensor
#18
Lanhua Liu, Xiaohong Zhou, Yun Lu, Didi Shan, Bi Xu, Miao He, Hanchang Shi, Yi Qian
The apparent increase in hormone-induced cancers and disorders of the reproductive tract has led to a growing demand for new technologies capable of screening xenoestrogens. We reported an estrogen receptor (ER)-based reusable fiber biosensor for facile screening estrogenic compounds in environment. The bioassay is based on the competition of xenoestrogens with 17β-estradiol (E2) for binding to the recombinant receptor of human estrogen receptor α (hERα) protein, leaving E2 free to bind to fluorophore-labeled anti-E2 monoclonal antibody...
May 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28549260/identification-of-oral-cavity-biofilm-forming-bacteria-and-determination-of-their-growth-inhibition-by-acacia-arabica-tamarix-aphylla-l-and-melia-azedarach-l-medicinal-plants
#19
Muhammad Khalid, Danial Hassani, Muhammad Bilal, Zahid Ali Butt, Muhammad Hamayun, Ayaz Ahmad, Danfeng Huang, Anwar Hussain
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability...
May 18, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28549259/purinergic-2x7-receptor-activation-regulates-wnt-signaling-in-human-mandibular-derived-osteoblasts
#20
Pimrumpai Rochanakit Sindhavajiva, Panunn Sastravaha, Mansuang Arksornnukit, Prasit Pavasant
OBJECTIVE: Purinergic 2X7 receptor (P2X7R) activation modulates in vitro mineralization by primary rat and human osteoblasts. However, the detailed mechanism of how P2X7R activation affects primary human osteoblasts remains unclear. The aim of this study was to investigate the effect of P2X7R activation on human mandibular-derived osteoblast (hMOB) differentiation. DESIGN: Primary human osteoblasts were obtained from non-pathologic mandibular bone from healthy patients...
May 18, 2017: Archives of Oral Biology
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