Jiabao Hu, Man Zhang, Kaiheng Yan, Youyi Zhang, Yaya Li, Jiajie Zhu, Guanlin Wang, Xiangbing Wang, Yuanbo Li, Xiang Huang, Jie Tang, Rongyue Zheng, Shanliang Xu, Danli Wang, Yajun Wang, Xiaojun Yan
We cultured silver pomfret for 20 days, decreasing water temperature from 18 to 8 ℃, and sampled muscle every 5 days. Muscle fiber degeneration and apoptosis began to increase at 13 ℃ detected by HE and TUNEL staining. Further analysis of transcriptome revealed that several apoptosis-related pathways were highly enriched by differentially expressed genes (DEGs). We analyzed 10 DEGs from these pathways by RT-qPCR during the temperature-decreasing process. JNK1, PIDD, CytC, Casp 3, and GADD45 were up-regulated after 15 and 20 days, while DUSP3, JNK2, and PARP genes were down-regulated after 15 and 20 days...
September 2, 2023: Marine Biotechnology