keyword
https://read.qxmd.com/read/38387719/effects-of-nitrous-oxide-and-ketamine-on-electrophysiological-and-molecular-responses-in-the-prefrontal-cortex-of-mice-a-comparative-study
#1
JOURNAL ARTICLE
Stanislav Rozov, Roosa Saarreharju, Stanislav Khirug, Markus Storvik, Claudio Rivera, Tomi Rantamäki
Nitrous oxide (N2 O; laughing gas) has recently reported to produce rapid antidepressant effects, but little is known about the underlying mechanisms. We performed transcriptomics, in situ hybridization, and electrophysiological studies to examine the potential shared signatures induced by 1 h inhalation of 50% N2 O and a single subanesthetic dose of ketamine (10 mg/kg, i.p.) in the medial prefrontal cortex (mPFC) in adult mice. Both treatments similarly affected the transcription of several negative regulators of mitogen-activated protein kinases (MAPKs), namely, dual specificity phosphatases...
February 20, 2024: European Journal of Pharmacology
https://read.qxmd.com/read/38300709/the-corest-repressor-complex-mediates-phenotype-switching-and-therapy-resistance-in-melanoma
#2
JOURNAL ARTICLE
Muzhou Wu, Ailish Hanly, Frederick Gibson, Robert Fisher, Samantha Rogers, Kihyun Park, Angelina Zuger, Kevin Kuang, Jay H Kalin, Sarah Nocco, Matthew Cole, Amy Xiao, Filisia Agus, Adam Labadorf, Samuel Beck, Marianne Collard, Philip A Cole, Rhoda M Alani
Virtually all patients with BRAF-mutant melanoma develop resistance to MAPK inhibitors largely through non-mutational events. Although the epigenetic landscape is shown to be altered in therapy-resistant melanomas and other cancers, a specific targetable epigenetic mechanism has not been validated to date. Here, we evaluate the CoREST repressor complex and the recently developed bivalent inhibitor, corin, within the context of melanoma phenotype plasticity and therapeutic resistance. We find that CoREST is a critical mediator of the major distinct melanoma phenotypes and that corin treatment of melanoma cells leads to phenotype reprogramming...
February 1, 2024: Journal of Clinical Investigation
https://read.qxmd.com/read/38260365/identifying-novel-gene-dysregulation-associated-with-opioid-overdose-death-a-meta-analysis-of-differential-gene-expression-in-human-prefrontal-cortex
#3
Javan K Carter, Bryan C Quach, Caryn Willis, Melyssa S Minto, Dana B Hancock, Janitza Montalvo-Ortiz, Olivia Corradin, Ryan W Logan, Consuelo Walss-Bass, Brion S Maher, Eric Otto Johnson
Only recently have human postmortem brain studies of differential gene expression (DGE) associated with opioid overdose death (OOD) been published; sample sizes from these studies have been modest (N = 40-153). To increase statistical power to identify OOD-associated genes, we leveraged human prefrontal cortex RNAseq data from four independent OOD studies and conducted a transcriptome-wide DGE meta-analysis (N = 285). Using a unified gene expression data processing and analysis framework across studies, we meta-analyzed 20LJ098 genes and found 335 significant differentially expressed genes (DEGs) by OOD status (false discovery rate < 0...
January 13, 2024: medRxiv
https://read.qxmd.com/read/38254666/proteomic-signaling-of-dual-specificity-phosphatase-4-dusp4-in-alzheimer-s-disease
#4
JOURNAL ARTICLE
Erming Wang, Allen L Pan, Pritha Bagchi, Srikant Rangaraju, Nicholas T Seyfried, Michelle E Ehrlich, Stephen R Salton, Bin Zhang
DUSP4 is a member of the DUSP (dual-specificity phosphatase) subfamily that is selective to the mitogen-activated protein kinases (MAPK) and has been implicated in a range of biological processes and functions in Alzheimer's disease (AD). In this study, we utilized the stereotactic delivery of adeno-associated virus (AAV)-DUSP4 to overexpress DUSP4 in the dorsal hippocampus of 5xFAD and wildtype (WT) mice, then used mass spectrometry (MS)-based proteomics along with the label-free quantification to profile the proteome and phosphoproteome in the hippocampus...
January 3, 2024: Biomolecules
https://read.qxmd.com/read/38187222/the-exploration-of-glucocorticoid-pathway-based-on-disease-severity-in-covid-19-patients
#5
JOURNAL ARTICLE
Gestina Aliska, Andani Eka Putra, Fenty Anggrainy, Mutia Lailani
Systemic inflammation is a hallmark of Coronavirus Disease 2019 (COVID-19) and is the key to the pathophysiology of its severe cases with host cytokine involvement. Glucocorticoids can moderate this inflammatory effect due to receptor binding (NRC31-the gene encoded), influencing the expression of effector genes and pro-inflammatory cytokines. Another important pathway in the processes of the immune and inflammatory responses is nuclear factor-κB (NF-κB) signaling (NFKBIA-the gene encoded). We aimed to explore the expression of genes in the glucocorticoid pathway in mild and severe COVID-19...
January 15, 2024: Heliyon
https://read.qxmd.com/read/38128678/dual-specificity-phosphatase-1-inhibits-singapore-grouper-iridovirus-replication-via-regulating-apoptosis-in-epinephelus-coioides
#6
JOURNAL ARTICLE
Jia-Yang He, Yi-Jie Cai, Yong-Xuan Li, Wei Huang, Yue Zhang, Ke-Jian Liu, Zhuo Sun, Li-Ling Dai, Qi-Wei Qi, Hong-Yan Sun
The dual-specificity phosphatase (DUSP) family plays key roles in the maintenance of cellular homeostasis and apoptosis etc. In this study, the DUSP member DUSP1 of Epinephelus coioides was characterized: the length was 2371 bp including 281 bp 5' UTR, 911 bp 3' UTR, and a 1125 bp open reading frame encoding 374 amino acids. E. coioides DUSP1 has two conserved domains, a ROHD and DSPc along with a p38 MAPK phosphorylation site, localized at Ser308. E. coioides DUSP1 mRNA can be detected in all of the tissues examined, and the subcellular localization showed that DUSP1 was mainly distributed in the nucleus...
December 19, 2023: Fish & Shellfish Immunology
https://read.qxmd.com/read/38106132/role-of-dusp-5-ko-on-vascular-properties-of-middle-cerebral-artery-in-rats
#7
Chengyun Tang, Huawei Zhang, Jane J Border, Yedan Liu, Xing Fang, Joshua R Jefferson, Andrew Gregory, Claire Johnson, Tae Jin Lee, Shan Bai, Ashok Sharma, Seung Min Shin, Hongwei Yu, Richard J Roman, Fan Fan
Vascular aging influences hemodynamics, elevating risks for vascular diseases and dementia. We recently demonstrated that knockout (KO) of Dusp5 enhances cerebral and renal hemodynamics and cognitive function. This improvement correlates with elevated pPKC and pERK1/2 levels in the brain and kidneys. Additionally, we observed that Dusp5 KO modulates the passive mechanical properties of cerebral and renal arterioles, associated with increased myogenic tone at low pressure, enhanced distensibility, greater compliance, and reduced stiffness...
December 6, 2023: bioRxiv
https://read.qxmd.com/read/37886598/proteomic-signaling-of-dual-specificity-phosphatase-4-dusp4-in-alzheimer-s-disease
#8
Erming Wang, Allen L Pan, Pritha Bagchi, Srikant Ranjaraju, Nicholas T Seyfried, Michelle E Ehrlich, Stephen R Salton, Bin Zhang
DUSP4 is a member of the DUSP (Dual-Specificity Phosphatase) subfamily that is selective to the mitogen-activated protein kinases (MAPK) and has been implicated in a range of biological processes and functions in Alzheimer's disease (AD). In this study, we utilized stereotactic delivery of adeno-associated virus (AAV)-DUSP4 to overexpress DUSP4 in the dorsal hippocampus of 5xFAD and wildtype (WT) mice, then used mass spectrometry (MS)-based proteomics along with label-free quantification to profile the proteome and phosphoproteome in the hippocampus...
October 19, 2023: Research Square
https://read.qxmd.com/read/37766214/the-role-of-epinephelus-coioides-dusp5-in-regulating-singapore-grouper-iridovirus-infection
#9
JOURNAL ARTICLE
Jiayang He, Yijie Cai, Wei Huang, Yunxiang Lin, Yurong Lei, Cuifen Huang, Zongbin Cui, Qiwei Qin, Hongyan Sun
The dual-specificity phosphatase (DUSP) family plays an important role in response to adverse external factors. In this study, the DUSP5 from Epinephelus coioides , an important marine fish in Southeast Asia and China, was isolated and characterized. As expected, E. coioides DUSP5 contained four conserved domains: a rhodanese homology domain (RHOD); a dual-specificity phosphatase catalytic domain (DSPc); and two regions of low compositional complexity, indicating that E. coioides DUSP5 belongs to the DUSP family...
August 25, 2023: Viruses
https://read.qxmd.com/read/37745468/proteomic-signaling-of-dual-specificity-phosphatase-4-dusp4-in-alzheimer-s-disease
#10
Erming Wang, Allen L Pan, Pritha Bagchi, Srikant Ranjaraju, Nicholas T Seyfried, Michelle E Ehrlich, Stephen R Salton, Bin Zhang
DUSP4 is a member of the DUSP (Dual-Specificity Phosphatase) subfamily that is selective to the mitogen-activated protein kinases (MAPK) and has been implicated in a range of biological processes and functions in Alzheimer's disease (AD). In this study, we utilized stereotactic delivery of adeno-associated virus (AAV)-DUSP4 to overexpress DUSP4 in the dorsal hippocampus of 5×FAD and wildtype (WT) mice, then used mass spectrometry (MS)-based proteomics along with label-free quantification to profile the proteome and phosphoproteome in the hippocampus...
September 14, 2023: bioRxiv
https://read.qxmd.com/read/37658990/cold-stress-induces-apoptosis-in-silver-pomfret-via-dusp-jnk-pathway
#11
JOURNAL ARTICLE
Jiabao Hu, Man Zhang, Kaiheng Yan, Youyi Zhang, Yaya Li, Jiajie Zhu, Guanlin Wang, Xiangbing Wang, Yuanbo Li, Xiang Huang, Jie Tang, Rongyue Zheng, Shanliang Xu, Danli Wang, Yajun Wang, Xiaojun Yan
We cultured silver pomfret for 20 days, decreasing water temperature from 18 to 8 ℃, and sampled muscle every 5 days. Muscle fiber degeneration and apoptosis began to increase at 13 ℃ detected by HE and TUNEL staining. Further analysis of transcriptome revealed that several apoptosis-related pathways were highly enriched by differentially expressed genes (DEGs). We analyzed 10 DEGs from these pathways by RT-qPCR during the temperature-decreasing process. JNK1, PIDD, CytC, Casp 3, and GADD45 were up-regulated after 15 and 20 days, while DUSP3, JNK2, and PARP genes were down-regulated after 15 and 20 days...
September 2, 2023: Marine Biotechnology
https://read.qxmd.com/read/37516290/the-dusp-domain-of-pseudophosphatase-mk-styx-interacts-with-g3bp1-to-decrease-stress-granules
#12
JOURNAL ARTICLE
Jonathan Smailys, Fei Jiang, Tatiana Prioleau, Kylan Kelley, Olivia Mitchell, Samah Nour, Lina Ali, William Buchser, Lynn Zavada, Shantá D Hinton
Mitogen activated protein kinase phosphoserine/threonine/tyrosine-binding protein (MK-STYX) is a dual specificity (DUSP) member of the protein tyrosine phosphatase family. It is a pseudophosphatase, which lacks the essential amino acids histidine and cysteine in the catalytic active signature motif (HCX5 R). We previously reported that MK-STYX interacts with G3BP1 [Ras-GAP (GTPase-activating protein) SH3 (Src homology 3) domain-binding-1] and reduces stress granules, stalled mRNA. To determine how MK-STYX reduces stress granules, truncated domains, CH2 (cell division cycle 25 phosphatase homology 2) and DUSP, of MK-STYX were used...
July 27, 2023: Archives of Biochemistry and Biophysics
https://read.qxmd.com/read/37511459/extracellular-heat-shock-protein-70-increases-the-glucocorticoid-receptor-and-dual-specificity-phosphatase-1-via-toll-like-receptor-4-and-attenuates-inflammation-in-airway-epithelial-cells
#13
JOURNAL ARTICLE
Liang Zhou, Lei Fang, Michael Tamm, Daiana Stolz, Michael Roth
Heat shock protein 70 (HSP70) regulates the ligand binding of the glucocorticoid receptor (GR). In asthma patients, heat treatment increased both the GR expression and secretion of extracellular HSP70 (eHSP70) by bronchial epithelial cells (EC). The objective of this study was to assess the effects of eHSP70 on GR expression and the GR-dependent regulation of immune response in human bronchial ECs. Cells were treated with either eHSP70 or transfected with an expression vector for intracellular HSP70 (iHSP70)...
July 20, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37493185/the-diverging-role-of-cdc14b-from-mitotic-exit-in-yeast-to-cell-fate-control-in-humans
#14
REVIEW
Patrick Partscht, Elmar Schiebel
CDC14, originally identified as crucial mediator of mitotic exit in budding yeast, belongs to the family of dual-specificity phosphatases (DUSPs) that are present in most eukaryotes. Contradicting data have sparked a contentious discussion whether a cell cycle role is conserved in the human paralogs CDC14A and CDC14B but possibly masked due to redundancy. Subsequent studies on CDC14A and CDC14B double knockouts in human and mouse demonstrated that CDC14 activity is dispensable for mitotic progression in higher eukaryotes and instead suggested functional specialization...
July 26, 2023: EMBO Journal
https://read.qxmd.com/read/37476896/expression-of-dual-specificity-phosphatases-in-tgf%C3%A3-1-induced-emt-in-skov3-cells
#15
JOURNAL ARTICLE
Sabire Güler, Abdullah Yalçın
BACKGROUND: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial- mesenchymal transition (EMT) in ovarian adenocarcinoma cells. METHODS: The ovarian adenocarcinoma cell line SKOV3 was used as a TGFβ1-induced EMT model. Cells were incubated with 5 ng/mL TGFβ1 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers...
June 2023: Turkish Journal of Medical Sciences
https://read.qxmd.com/read/37409507/revisiting-vh1-phosphatase-at-the-time-of-monkeypox-back-to-the-spotlight
#16
JOURNAL ARTICLE
Angel Bottini, Diana R D C G Pacheco, Fabio L Forti, Nunzio Bottini
Vaccinia virus is a poxvirus that has been successfully leveraged to develop vaccines for smallpox, which is caused by the closely related Variola virus. Smallpox has been declared as 'eradicated' by the WHO in 1980; however, it still poses a potential bioterrorism threat. More recently, the spreading of monkeypox (MPox) in non-endemic countries has further highlighted the importance of continuing the exploration for druggable targets for poxvirus infections. The vaccinia H1 (VH1) phosphatase is the first reported dual specificity phosphatase (DUSP) able to hydrolyze both phosphotyrosine and phosphoserine/phosphotheonine residues...
July 6, 2023: Biochemical Society Transactions
https://read.qxmd.com/read/37291053/quercetin-induces-dual-specificity-phosphatase-5-via-serum-response-factor
#17
Kanokkan Boonruang, Ilju Kim, Chaeyoung Kwag, Junsun Ryu, Seung Joon Baek
The phytochemical quercetin has gained attention for its anti-inflammatory and anti-tumorigenic properties in various types of cancer. Tumorigenesis involves aberrant regulation of kinase/phosphatase, highlighting the importance of maintaining homeostasis. Dual Specificity Phosphatase (DUSPs) plays a crucial role in controlling the phosphorylation of ERK. The current study aimed to clone the DUSP5 promoter and investigate its transcriptional activity in the presence of quercetin. The results revealed that quercetin-induced DUSP5 expression is associated with the serum response factor (SRF) binding site located in the DUSP5 promoter...
June 9, 2023: BMB Reports
https://read.qxmd.com/read/37195197/proteogenomic-and-v-d-j-analysis-of-human-decidual-t-cells-highlights-unique-transcriptional-programming-and-clonal-distribution
#18
JOURNAL ARTICLE
Deborah A Chasman, Rene Welch Schwartz, Jessica Vazquez, Melina Chavarria, Eryne T Jenkins, Gladys E Lopez, Chanel T Tyler, Aleksandar K Stanic, Irene M Ong
Immunological tolerance toward the semiallogeneic fetus is one of many maternal adaptations required for a successful pregnancy. T cells are major players of the adaptive immune system and balance tolerance and protection at the maternal-fetal interface; however, their repertoire and subset programming are still poorly understood. Using emerging single-cell RNA sequencing technologies, we simultaneously obtained transcript, limited protein, and receptor repertoire at the single-cell level, from decidual and matched maternal peripheral human T cells...
May 17, 2023: Journal of Immunology
https://read.qxmd.com/read/37160873/tfeb-inhibition-induces-melanoma-shut-down-by-blocking-the-cell-cycle-and-rewiring-metabolism
#19
JOURNAL ARTICLE
C Ariano, F Costanza, M Akman, C Riganti, D Corà, E Casanova, E Astanina, V Comunanza, F Bussolino, G Doronzo
Melanomas are characterised by accelerated cell proliferation and metabolic reprogramming resulting from the contemporary dysregulation of the MAPK pathway, glycolysis and the tricarboxylic acid (TCA) cycle. Here, we suggest that the oncogenic transcription factor EB (TFEB), a key regulator of lysosomal biogenesis and function, controls melanoma tumour growth through a transcriptional programme targeting ERK1/2 activity and glucose, glutamine and cholesterol metabolism. Mechanistically, TFEB binds and negatively regulates the promoter of DUSP-1, which dephosphorylates ERK1/2...
May 9, 2023: Cell Death & Disease
https://read.qxmd.com/read/37073906/the-sars-cov-2-virus-could-downregulate-dual-specificity-phosphatase-1-expression-in-oral-cancer-a-plausible-hypothesis-that-could-explain-oral-cancer-progression-in-the-covid-19-pandemic-and-post-pandemic-situation
#20
JOURNAL ARTICLE
Rajeswari Ramesh, Pearlcid Siroraj, Saranya Varadarajan, Thodur Madapusi Balaji, Shankargouda Patil
This study explores the downregulation of Dual Specificity Phosphatase-1 (DUSP-1) expression in oral cancer progression during the pandemic and post-pandemic situations. Keywords: COVID-19, Dual specificity phosphatase-1, Oral cancer, SARS-CoV-2.
October 1, 2022: Journal of Contemporary Dental Practice
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