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https://www.readbyqxmd.com/read/27905550/deep-sequencing-of-transcriptome-profiling-of-gstm2-knock-down-in-swine-testis-cells
#1
Yuqi Lv, Yi Jin, Yongqiang Zhou, Jianjun Jin, Zhenfa Ma, Zhuqing Ren
Glutathione-S-transferases mu 2 (GSTM2), a kind of important Phase II antioxidant enzyme of eukaryotes, is degraded by nonsense mediated mRNA decay due to a C27T substitution in the fifth exon of pigs. As a reproductive performance-related gene, GSTM2 is involved in embryo implantation, whereas, functional deficiency of GSTM2 induces pre- or post-natal death in piglets potentially. To have some insight into the role of GSTM2 in embryo development, high throughput RNA sequencing is performed using the swine testis cells (ST) with the deletion of GSTM2...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888805/effects-of-abies-sibirica-terpenes-on-cancer-and-aging-associated-pathways-in-human-cells
#2
Anna Kudryavtseva, George Krasnov, Anastasiya Lipatova, Boris Alekseev, Faniya Maganova, Mikhail Shaposhnikov, Maria Fedorova, Anastasiya Snezhkina, Alexey Moskalev
A large number of terpenoids exhibit potential geroprotector and anti-cancer properties. Here, we studied whole transcriptomic effects of Abisil, the extract of fir (Abies sibirica) terpenes, on normal and cancer cell lines. We used early passaged and senescent none-immortalized fibroblasts as cellular aging models. It was revealed that in normal fibroblasts, terpenes induced genes of stress response, apoptosis regulation and tissue regeneration. The restoration of the expression level of some prolongevity genes after fir extract treatment was shown in old cells...
November 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27739308/critical-role-of-the-secondary-binding-pocket-in-modulating-enzymatic-activity-of-dusp5-towards-phosphorylated-erks
#3
Marat R Talipov, Jaladhi Nayak, Michael Lepley, Robert D Bongard, Daniel S Sem, Ramani Ramchandran, Rajendra Rathore
DUSP5 is an inducible nuclear dual-specificity phosphatase that specifically interacts with and deactivates extracellular signal-regulated kinases ERK1 and ERK2, which are responsible for cell proliferation, differentiation, and survival. The phosphatase domain (PD) of DUSP5 has unique structural features absent in other nuclear DUSPs, such as presence of a secondary anion-binding site in the proximity to the reaction center and a glutamic acid E264 positioned next to the catalytic cysteine C263, as well as a remote intra-molecular disulfide linkage...
October 14, 2016: Biochemistry
https://www.readbyqxmd.com/read/27713552/differential-transcriptional-responses-to-ebola-and-marburg-virus-infection-in-bat-and-human-cells
#4
Martin Hölzer, Verena Krähling, Fabian Amman, Emanuel Barth, Stephan H Bernhart, Victor A O Carmelo, Maximilian Collatz, Gero Doose, Florian Eggenhofer, Jan Ewald, Jörg Fallmann, Lasse M Feldhahn, Markus Fricke, Juliane Gebauer, Andreas J Gruber, Franziska Hufsky, Henrike Indrischek, Sabina Kanton, Jörg Linde, Nelly Mostajo, Roman Ochsenreiter, Konstantin Riege, Lorena Rivarola-Duarte, Abdullah H Sahyoun, Sita J Saunders, Stefan E Seemann, Andrea Tanzer, Bertram Vogel, Stefanie Wehner, Michael T Wolfinger, Rolf Backofen, Jan Gorodkin, Ivo Grosse, Ivo Hofacker, Steve Hoffmann, Christoph Kaleta, Peter F Stadler, Stephan Becker, Manja Marz
The unprecedented outbreak of Ebola in West Africa resulted in over 28,000 cases and 11,000 deaths, underlining the need for a better understanding of the biology of this highly pathogenic virus to develop specific counter strategies. Two filoviruses, the Ebola and Marburg viruses, result in a severe and often fatal infection in humans. However, bats are natural hosts and survive filovirus infections without obvious symptoms. The molecular basis of this striking difference in the response to filovirus infections is not well understood...
October 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27702885/dual-specific-phosphatase-12-ameliorates-cardiac-hypertrophy-in-response-to-pressure-overload
#5
Wei-Ming Li, Yi-Fan Zhao, Guo-Fu Zhu, Wen-Hui Peng, Meng-Yun Zhu, Xue-Jing Yu, Wei Chen, Da-Chun Xu, Ya-Wei Xu
BACKGROUND: Pathological cardiac hypertrophy is an independent risk factor of heart failure. However, we still lack effective methods to reverse cardiac hypertrophy. DUSP12 is a member of the DUSP family, which is characterized by its dual specific phosphatase activity to dephosphorylate both tyrosine and serine/threonine residues on one substrate. Some DUSPs have been identified as being involved in the regulation of cardiac hypertrophy. However, the role of DUSP12 during pathological cardiac hypertrophy is still unclear...
October 4, 2016: Clinical Science (1979-)
https://www.readbyqxmd.com/read/27696308/microrna-200c-modulates-dusp-1-expression-in-diabetes-induced-cardiac-hypertrophy
#6
Gurinder Bir Singh, Satish K Raut, Sanskriti Khanna, Akhilesh Kumar, Saurabh Sharma, Rishikesh Prasad, Madhu Khullar
Mitogen-activated protein kinases (MAPKs) (ERK1/2, JNK, and p38) are upregulated in diabetic cardiomyopathy (DCM). Dual-specific phosphatase-1 (DUSP-1) has been reported to regulate the activity of MAPKs in cardiac hypertrophy; however, the role of DUSP-1 in regulating MAPKs activity in DCM is not known. MicroRNAs have been reported to regulate the expression of several genes in hypertrophied failing hearts. However, little is known about the microRNAs regulating DUSP-1 expression in diabetes-related cardiac hypertrophy...
September 30, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27575022/regulation-of-cardiac-hypertrophy-and-remodeling-through-the-dual-specificity-mapk-phosphatases-dusps
#7
Ruijie Liu, Jeffery D Molkentin
Mitogen-activated protein kinases (MAPKs) play a critical role in regulating cardiac hypertrophy and remodeling in response to increased workload or pathological insults. The spatiotemporal activities and inactivation of MAPKs are tightly controlled by a family of dual-specificity MAPK phosphatases (DUSPs). Over the past 2 decades, we and others have determined the critical role for selected DUSP family members in controlling MAPK activity in the heart and the ensuing effects on ventricular growth and remodeling...
August 27, 2016: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/27514808/visualizing-and-quantitating-the-spatiotemporal-regulation-of-ras-erk-signaling-by-dual-specificity-mitogen-activated-protein-phosphatases-mkps
#8
Christopher J Caunt, Andrew M Kidger, Stephen M Keyse
The spatiotemporal regulation of the Ras/ERK pathway is critical in determining the physiological and pathophysiological outcome of signaling. Dual-specificity mitogen-activated protein kinase (MAPK) phosphatases (DUSPs or MKPs) are key regulators of pathway activity and may also localize ERK to distinct subcellular locations. Here we present methods largely based on the use of high content microscopy to both visualize and quantitate the subcellular distribution of activated (p-ERK) and total ERK in populations of mouse embryonic fibroblasts derived from mice lacking DUSP5, a nuclear ERK-specific MKP...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27475814/retraction-statement-paper-by-qian-y-ma-j-guo-x-sun-j-yu-y-caob-zhang-l-ding-x-huang-j-shao-jf-entitled-curcumin-enhances-the-radiosensitivity-ofu87-cells-by-inducing-dusp-2-up-regulation-cell-physiol-biochem-2015-35-4-1381-93-doi-10-1159-000373959
#9
https://www.readbyqxmd.com/read/27459239/role-for-dusp1-dual-specificity-protein-phosphatase-1-in-the-regulation-of-autophagy
#10
Juan Wang, Jun-Ying Zhou, Dhonghyo Kho, John J Reiners, Gen Sheng Wu
Accumulating evidence suggests that mitogen-activated protein kinases (MAPKs) regulate macroautophagy/autophagy. However, the involvement of dual-specificity protein phosphatases (DUSPs), endogenous inhibitors for MAPKs, in autophagy remains to be determined. Here we report that DUSP1/MKP-1, the founding member of the DUSP family, plays a critical role in regulating autophagy. Specifically, we demonstrate that DUSP1 knockdown by shRNA in human ovarian cancer CAOV3 cells and knockout in murine embryonic fibroblasts, increases both basal and rapamycin-increased autophagic flux...
October 2, 2016: Autophagy
https://www.readbyqxmd.com/read/27422819/follicle-stimulating-hormone-fsh-dependent-regulation-of-extracellular-regulated-kinase-erk-phosphorylation-by-the-mitogen-activated-protein-map-kinase-phosphatase-mkp3
#11
Elyse M Donaubauer, Nathan C Law, Mary E Hunzicker-Dunn
Within the ovarian follicle, granulosa cells (GCs) surround and support immature oocytes. FSH promotes the differentiation and proliferation of GCs and is essential for fertility. We recently reported that ERK activation is necessary for FSH to induce key genes that define the preovulatory GC. This research focused on the phosphoregulation by FSH of ERK within GCs. FSH-stimulated ERK phosphorylation on Thr(202)/Tyr(204) was PKA-dependent, but MEK(Ser(217)/Ser(221)) phosphorylation was not regulated; rather, MEK was already active...
September 9, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27280600/genomic-and-transcriptomic-profiles-and-in-vitro-resistance-to-mitoxantrone-and-idarubicin-in-pediatric-acute-leukemias
#12
Joanna Laskowska, Joanna Lewandowska-Bieniek, Joanna Szczepanek, Jan Styczyński, Andrzej Tretyn
BACKGROUND: A major problem in the treatment of leukemia is the development of drug resistance to chemotherapeutic agents. METHODS: To determine the ex vivo drug resistance profile to anthracyclines, an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) cytotoxicity assay was performed on mononuclear cells obtained from 155 patients with acute lymphoblastic leukemia (ALL) or acute myeloblastic leukemia (AML). Gene expression profiles (for 51 patients with ALL and 16 with AML) were prepared on the basis of cRNA hybridization to oligonucleotide arrays of the human genome (Affymetrix)...
August 2016: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27255711/structural-basis-of-the-recruitment-of-ubiquitin-specific-protease-usp15-by-spliceosome-recycling-factor-sart3
#13
Qi Zhang, Rachel Harding, Feng Hou, Aiping Dong, John R Walker, Joseph Bteich, Yufeng Tong
Ubiquitin-specific proteases (USPs) USP15 and USP4 belong to a subset of USPs featuring an N-terminal tandem domain in USP (DUSP) and ubiquitin-like (UBL) domain. Squamous cell carcinoma antigen recognized by T-cell 3 (SART3), a spliceosome recycling factor, binds to the DUSP-UBL domain of USP15 and USP4, recruiting them to the nucleus from the cytosol to control deubiquitination of histone H2B and spliceosomal proteins, respectively. To provide structural insight, we solved crystal structures of SART3 in the apo-form and in complex with the DUSP-UBL domain of USP15 at 2...
August 12, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27225478/dusp8-regulates-cardiac-ventricular-remodeling-by-altering-erk1-2-signaling
#14
Ruijie Liu, Jop H van Berlo, Allen J York, Ronald J Vagnozzi, Marjorie Maillet, Jeffery D Molkentin
RATIONALE: Mitogen-activated protein kinase (MAPK) signaling regulates the growth response of the adult myocardium in response to increased cardiac workload or pathological insults. The dual-specificity phosphatases (DUSPs) are critical effectors, which dephosphorylate the MAPKs to control the basal tone, amplitude, and duration of MAPK signaling. OBJECTIVE: To examine DUSP8 as a regulator of MAPK signaling in the heart and its impact on ventricular and cardiac myocyte growth dynamics...
July 8, 2016: Circulation Research
https://www.readbyqxmd.com/read/27162525/regulatory-roles-of-mapk-phosphatases-in-cancer
#15
REVIEW
Heng Boon Low, Yongliang Zhang
The mitogen-activated protein kinases (MAPKs) are key regulators of cell growth and survival in physiological and pathological processes. Aberrant MAPK signaling plays a critical role in the development and progression of human cancer, as well as in determining responses to cancer treatment. The MAPK phosphatases (MKPs), also known as dual-specificity phosphatases (DUSPs), are a family of proteins that function as major negative regulators of MAPK activities in mammalian cells. Studies using mice deficient in specific MKPs including MKP1/DUSP1, PAC-1/DUSP2, MKP2/DUSP4, MKP5/DUSP10 and MKP7/DUSP16 demonstrated that these molecules are important not only for both innate and adaptive immune responses, but also for metabolic homeostasis...
April 2016: Immune Network
https://www.readbyqxmd.com/read/27066978/adenosine-a2a-receptor-signaling-attenuates-lps-induced-pro-inflammatory-cytokine-formation-of-mouse-macrophages-by-inducing-the-expression-of-dusp1
#16
Krisztina Köröskényi, Beáta Kiss, Zsuzsa Szondy
Adenosine is known to reduce inflammation by suppressing the activity of most immune cells. Previous studies have shown that lipopolysaccharide (LPS) stimulated mouse macrophages produce adenosine, and the adenosine A2A receptor (A2AR) signaling activated in an autocrine manner attenuates LPS-induced pro-inflammatory cytokine formation. It has been suggested that A2AR signaling inhibits LPS-induced pro-inflammatory cytokine production through a unique cAMP-dependent, but PKA- and Epac-independent signaling pathway...
July 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27060135/structural-basis-for-recruiting-and-shuttling-of-the-spliceosomal-deubiquitinase-usp4-by-sart3
#17
Joon Kyu Park, Tanuza Das, Eun Joo Song, Eunice EunKyeong Kim
Squamous cell carcinoma antigen recognized by T-cells 3 (SART3) is a U4/U6 recycling factor as well as a targeting factor of USP4 and USP15. However, the details of how SART3 recognizes these deubiquitinases and how they get subsequently translocated into the nucleus are not known. Here, we present the crystal structures of the SART3 half-a-tetratricopeptide (HAT) repeat domain alone and in complex with the domain present in ubiquitin-specific protease (DUSP)-ubiquitin-like (UBL) domains of ubiquitin specific protease 4 (USP4)...
June 20, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27050915/p38mapk-activation-and-dusp10-expression-in-meningiomas
#18
Mahlon D Johnson, Jay E Reeder, Mary O'Connell
The mitogen activated protein kinase (MAPK) p38MAPK has been implicated in regulation of cell proliferation and apoptosis. However, expression, activation and regulation has not been studied in meningiomas, to our knowledge. p38MAPK is regulated, in part, by dual specificity phosphatases (DUSP) that inactivate signaling by dephosphorylation. DUSP10 is also a likely participant in regulating meningioma proliferation. Five fetal and an adult human leptomeninges and 37 meningioma cultures (MC) were evaluated for DUSP10 as well as phosphorylation of its substrates p38MAPK and p44/42MAPK by western blot and DUSP10 expression by polymerase chain reaction...
August 2016: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/27050744/specific-induction-of-tslp-by-the-viral-rna-analogue-poly-i-c-in-primary-epithelial-cells-derived-from-nasal-polyps
#19
Korneliusz Golebski, Joost van Tongeren, Danielle van Egmond, Esther J de Groot, Wytske J Fokkens, Cornelis M van Drunen
INTRODUCTION: Chronic rhinosinusitis with nasal polyposis is an inflammatory disease that, although not directly linked to allergy, often displays a Th2-skewed inflammation characterized by elevated local IgE and IL-5 levels. The nasal cavity is constantly exposed to bacteria and viruses that may trigger epithelial inflammatory responses. To gain more insight into mechanisms by which such a biased inflammation might arise, we have investigated the epithelial expression of the Th2 skewing mediators (TSLP, IL-25, and IL-33) in relationship to disease and microbial triggers...
2016: PloS One
https://www.readbyqxmd.com/read/26859151/differential-roles-for-dusp-family-members-in-epithelial-to-mesenchymal-transition-and-cancer-stem-cell-regulation-in-breast-cancer
#20
Tara Boulding, Fan Wu, Robert McCuaig, Jennifer Dunn, Christopher R Sutton, Kristine Hardy, Wenjuan Tu, Amanda Bullman, Desmond Yip, Jane E Dahlstrom, Sudha Rao
Dual-specificity phosphatases (DUSPs) dephosphorylate threonine/serine and tyrosine residues on their substrates. Here we show that DUSP1, DUSP4, and DUSP6 are involved in epithelial-to-mesenchymal transition (EMT) and breast cancer stem cell (CSC) regulation. DUSP1, DUSP4, and DUSP6 are induced during EMT in a PKC pathway signal-mediated EMT model. We show for the first time that the key chromatin-associated kinase PKC-θ directly regulates a subset of DUSP family members. DUSP1, DUSP4, and DUSP6 globally but differentially co-exist with enhancer and permissive active histone post-translational modifications, suggesting that they play distinct roles in gene regulation in EMT/CSCs...
2016: PloS One
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