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Ribose AND glycation

Rabia Nabi, Sahir Sultan Alvi, Rizwan Khan, Shafeeque Ahmad, Saheem Ahmad, M Salman Khan
Non-enzymatic glycation mediated advanced glycation end products (AGEs) generation results in the pathogenesis of diabetic complications and atherosclerotic cardiovascular disease (ASCVD) which is greatly influenced by 3-hydroxy-3-methyl-glutaryl Co-A reductase (HMG-R) activity. HMG-R inhibitors, statins, are well known for reducing mortality and morbidity of ASCVD in patients with diabetes due to their pleiotropic effects independent of cholesterol lowering. Due to distinct chemical structures, various statins may play important role in the inhibition of AGEs mediated pathologies...
May 18, 2018: International Journal of Biological Macromolecules
U Daood, J K H Tsoi, P Neelakantan, J P Matinlinna, H A K Omar, M Al-Nabulsi, A S Fawzy
OBJECTIVE: Collagen fibrils aid in anchoring resin composite restorations to the dentine substrate. The aim of the study was to investigate effect of non-enzymatic glycation on bond strength and durability of demineralized dentine specimens in a modified two-step etch-and-rinse dentine adhesive. METHODS: Dentine surfaces were etched with 37% phosphoric acid, bonded with respective in vitro ethanol and acetone adhesives modified with (m/m, 0, 1%, 2% and 3% ribose), restored with restorative composite-resin, and sectioned into resin-dentine slabs and beams to be stored for 24h or 12 months in artificial saliva...
May 17, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Mustafa Unal, Sasidhar Uppuganti, Calen J Leverant, Amy Creecy, Mathilde Granke, Paul Voziyan, Jeffry S Nyman
Establishing a non-destructive method for spatially assessing advanced glycation end-products (AGEs) is a potentially useful step toward investigating the mechanistic role of AGEs in bone quality. To test the hypothesis that the shape of the Amide I in the Raman Spectroscopy (RS) analysis of bone matrix changes upon AGE accumulation, we incubated paired cadaveric cortical bone in ribose or glucose solutions and in control solutions for 4 and 16 weeks, respectively, at 37 °C. Acquiring 10 spectra per bone with a 20X objective and a 830 nm laser, RS was sensitive to AGE accumulation (confirmed by biochemical measurements of pentosidine and fluorescent AGEs)...
March 25, 2018: Journal of Biophotonics
Guang-Xian Liu, Zong-Cai Tu, Wenhua Yang, Hui Wang, Lu Zhang, Da Ma, Tao Huang, Jun Liu, Xue Li
Ultrasound treatment could change the conformation of β-lactoglobulin (β-Lg) and improve the glycation reaction in aqueous solution under neutral condition. However, the effect of ultrasound pretreatment on glycation of β-Lg with pentose at dry-state remains ambiguous, and the relationship between glycation and allergenicity of β-Lg with ultrasound pretreatment is unclear. This study aimed to evaluate the effect of ultrasound pretreatment on glycation and allergenicity of β-Lg. Markedly decreased allergenicity of β-Lg was observed after glycation with ribose before and after ultrasound pretreatment with the minimum found at 400 W...
June 30, 2018: Food Chemistry
Ishrat Jahan Saifi, Km Neelofar, M R Ajmal, Sheelu Shafiq Siddiqi
Glycoxidation of protein may lead to develop diabetes. In the present study, different concentrations of 2-deoxy d-ribose (2-dRib) were used to modify human serum albumin (HSA). Nitro Blue Tetrazolium (NBT) assay results showed that yield of the fructosamine content was directly proportional to the concentration of 2-dRib. UV and fluorescence spectroscopy results showed an increment in hyperchromicity and decrease in fluorescence intensity of 2-dRib modified HSA as compared to native HSA. Further secondary structural changes were confirmed by UV-circular dichroism (UV-CD) and Fourier transform infrared spectroscopy (FT-IR)...
July 1, 2018: International Journal of Biological Macromolecules
Mariarosa Maietta, Raffaella Colombo, Federica Corana, Adele Papetti
Accumulation of advanced glycation end products (AGEs) in vivo is associated with many chronic disorders such as diabetes, renal failure, aging, and Alzheimer's disease. The aim of this study was to expand the knowledge about the functional properties of Origanum dictamnus L. beverage (Cretan tea) by an investigation about the inhibitory effects on the formation of AGEs and the capacity to trap dicarbonyl compounds. Dittany infusion was characterized for its polyphenolic composition by RP-HPLC-DAD-ESI/MSn and twenty compounds were detected...
March 1, 2018: Food & Function
Beibei Wu, Lexiang Yu, Pingdong Hu, Yang Lu, Juan Li, Yan Wei, Rongqiao He
d-Ribose (Rib), a reactive glycation compound that exists in organisms, abnormally increases in the urine of diabetic patients and can yield large amounts of advanced glycation end products (AGEs), leading to cell dysfunction. However, whether cellular proteins are sensitive to this type of glycation is unknown. In this study, we found that cellular AGEs accumulate in Chinese hamster ovary (CHO) cells with increased Rib concentration and administration time. Mass spectrum analysis of isolated AGE-modified proteins from cell lysates showed that glucose-regulated protein 78 kD (GRP78) is one of the main ribosylated proteins...
April 2018: Biochimica et Biophysica Acta
Alexander Klaus, Tim Baldensperger, Roman Fiedler, Matthias Girndt, Marcus A Glomb
In the present study, we investigated the role of transketolase (TK) in the modulation of glycolaldehyde driven Maillard reactions. In vitro experiments with recombinant human TK reduced glycolaldehyde and glyoxal induced carbonyl stress and thereby suppressed the formation of advanced glycation endproducts up to 70% due to the enzyme-catalyzed conversion of glycolaldehyde to erythrulose. This was further substantiated by the use of 13 C-labeled compounds. For the first time, glycolaldehyde and other sugars involved in the TK reaction were quantified in vivo and compared to nondiabetic uremic patients undergoing hemodialysis...
February 14, 2018: Journal of Agricultural and Food Chemistry
Zeba Siddiqui, Mohd Ishtikhar, Moinuddin, Saheem Ahmad
Glycation of biological macromolecule leads to the establishment of advanced glycation end products (AGEs) having implications in metabolic disorders. d‑ribose appears to be the most reactive among the naturally occurring sugars and contribute significantly to the glycation reactions in vivo, however, no report have been published yet to discuss d‑ribose induced glycation of hemoglobin (Hb). Therefore, the present study was designed to investigate d‑ribose induced glycoxidative damage to Hb protein. Briefly, the commercially available Hb was glycated with d‑ribose for varying time intervals...
June 2018: International Journal of Biological Macromolecules
Nanhai Zhang, Zongcai Tu, Hui Wang, Guangxian Liu, Zhenxing Wang, Tao Huang, Xu Qin, Xing Xie, A'mei Wang
Ultrasonication is an emerging technology applied in food processing and biological experimental pretreatments. Cavitation phenomena induced during ultrasonic treatment can generate localized high temperature and pressure, which can result in glycation reaction between protein and reducing sugars. In this study, the mixture of bovine serum albumin (BSA) and d-ribose was treated under 600 W for different times. Interestingly, a large amount of carbonized black materials appeared after ultrasonication, while the UV absorbance and intrinsic fluorescence spectra reflecting conformational changes were not obvious...
January 24, 2018: Journal of Agricultural and Food Chemistry
Guillaume A W Beaudoin, Qiang Li, Steven D Bruner, Andrew D Hanson
Flavins are notoriously photolabile, but while the photoproducts derived from the iso -alloxazine ring are well known the other photoproducts are not. In the case of FAD, typically the main cellular flavin, the other photoproducts are predicted to include four- and five-carbon sugars linked to ADP. These FAD photoproducts were shown to be potent glycating agents, more so than ADP-ribose. Such toxic compounds would require disposal via an ADP-sugar diphosphatase or other route. Comparative analysis of bacterial genomes uncovered a candidate disposal gene that is chromosomally clustered with genes for FAD synthesis or transport and is predicted to encode a protein of the PhnP cyclic phosphodiesterase family...
January 11, 2018: Biochemical Journal
R Li, R Rajan, W C V Wong, D G Reid, M J Duer, V J Somovilla, N Martinez-Saez, G J L Bernardes, R Hayward, C M Shanahan
Non-enzymatic glycation of extracellular matrix with (U-13 C5 )-d-ribose-5-phosphate (R5P), enables in situ 2D ssNMR identification of many deleterious protein modifications and crosslinks, including previously unreported oxalamido and hemiaminal (CH3 -CH(OH)NHR) substructures. Changes in charged residue proportions and distribution may be as important as crosslinking in provoking and understanding harmful tissue changes.
December 14, 2017: Chemical Communications: Chem Comm
P Ramlagan, P Rondeau, C Planesse, V S Neergheen-Bhujun, E Bourdon, T Bahorun
This study aimed at investigating and comparing the anti-diabetic potential of black and green teas. Biochemical analyses indicate higher antioxidant potency, significantly correlated with the phytochemicals present, in green teas compared to black teas. Both extracts afforded a similar level of protection to erythrocytes against peroxyl radical-induced lysis. Non-cytotoxic concentration of green and black tea extracts significantly reduced the reactive oxygen species (ROS) production (P < 0.01), lowered the oxidation of proteins (P < 0...
October 18, 2017: Food & Function
Sebastian M Fica-Contreras, Sydney O Shuster, Nathaniel D Durfee, Gregory J K Bowe, Nathaniel J Henning, Staci A Hill, Geoffrey D Vrla, David R Stillman, Kelly M Suralik, Roger K Sandwick, Sunhee Choi
Extensive research has linked the amyloid-beta (Aβ) peptide to neurological dysfunction in Alzheimer's disease (AD). Insoluble Aβ plaques in the AD patient brain contain high concentrations of advanced glycation end-products (AGEs) as well as transition metal ions. This research elucidated the roles of Aβ, sugars, and Cu2+ in the oxidative stress mechanism of AD at the molecular level. Mass spectral (MS) analysis of the reactions of Aβ with two representative sugars, ribose-5-phosphate (R5P) and methylglyoxal (MG), revealed Lys-16 and Arg-5 as the primary glycation sites...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Xixi Chen, Tao Su, Yao Chen, Yingge He, Ying Liu, Yong Xu, Yan Wei, Juan Li, Rongqiao He
Glycated haemoglobin (HbA1c) is the most important marker of hyperglycaemia in diabetes mellitus. We show that d-ribose reacts with haemoglobin, thus yielding HbA1c. Using mass spectrometry, we detected glycation of haemoglobin with d-ribose produces 10 carboxylmethyllysines (CMLs). The first-order rate constant of fructosamine formation for d-ribose was approximately 60 times higher than that for d-glucose at the initial stage. Zucker Diabetic Fatty (ZDF) rat, a common model for type 2 diabetes mellitus (T2DM), had high levels of d-ribose and HbA1c, accompanied by a decrease of transketolase (TK) in the liver...
November 2017: EBioMedicine
Rudolf Flückiger, Enzo Cocuzzi, Ram H Nagaraj, Menachem Shoham, Timothy S Kern, M Edward Medof
Decay accelerating factor (DAF or CD55) is a cell associated C3 and C5 convertase regulator originally described in terms of protection of self-cells from systemic complement but now known to modulate adaptive T cell responses. It is expressed on all cell types. We investigated whether nonenzymatic glycation could impair its function and potentially be relevant to complications of diabetes mellitus and other conditions that result in nonenzymatic glycation including cancer, Alzheimer's disease, and aging. Immunoblots of affinity-purified DAF from erythrocytes of patients with diabetes showed pentosidine, glyoxal-AGEs, carboxymethyllysine, and argpyrimidine...
January 2018: Molecular Immunology
Mariarosa Maietta, Raffaella Colombo, Roberto Lavecchia, Milena Sorrenti, Antonio Zuorro, Adele Papetti
The role of polyphenolic compounds extractable from artichoke solid wastes in the formation of advanced glycation end products (AGEs) was studied. Outer bracts and stems were extracted using different water-ethanol mixtures and HPLC-DAD analyses indicated aqueous and hydro-alcoholic 20:80 stem extracts as the richest in polyphenols. The samples were characterized in their phenolic composition (using mass spectrometry) and antioxidant capacity. Antiglycative capacity was evaluated by in vitro BSA-sugars (glucose, fructose, and ribose) and BSA-methylglyoxal (MGO) tests, formation of Amadori products assay, direct glyoxal (GO) and MGO trapping capacity...
October 2017: Food Research International
Junya Ozawa, Akinori Kaneguchi, Kengo Minamimoto, Ryo Tanaka, Nobuhiro Kito, Hideki Moriyama
Joint mobility decreases in the elderly and in diabetics, this process is thought to be caused by accumulation of advanced-glycation end products (AGEs). Here, we aimed to elucidate the role of AGEs in joint contracture formation in rat knees. Rats were injected with ribose or saline into the knees twice weekly for eight weeks. Pentosidine (AGE) levels were measured in the knee-joint tissues. After serial injections, rats were subjected to unilateral knee-joint immobilization in a flexion position for various periods...
September 1, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Peerzada Shariq Shaheen Khaki, Anna Feroz, Azad Alam Siddiqui, Syed Mudasir Qadri, Fakhra Amin, Bilqees Bano
Glycation induced advanced glycation end products (AGEs) of proteins formed as a result of Maillard reaction is currently at the heart of a number of pathological conditions. The formation of chemically stable AGEs can permanently alter protein structure and function; hence can serve as an implication in long term complications. Cystatins with high amyloidogenic inclination are implicated in various diseases including cancer and neurodegenerative conditions. The aggregates of cystatin purified from caprine brain have been studied on addition of glucose and ribose using UV absorption, fluorescence emission, circular dichroism (CD) spectroscopy and transmission electron microscopy (TEM)...
January 2018: International Journal of Biological Macromolecules
Vítor Spínola, Paula C Castilho
The study was performed to assess, for the first time, the in vitro anti-diabetic potential of ten Asteraceae plant extracts to inhibit the activity of digestive enzymes (α-amylase, α-, β-glucosidases and lipase) responsible for hydrolysis/digestion of sugar and lipids. Prevention of advanced glycation end-products (AGEs) formation was evaluated in bovine serum albumin/ribose glycation reaction model. The phytochemical profiles and caffeoylquinic acids (CQAs) contents were determined for the methanolic extract of each plant...
November 2017: Phytochemistry
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