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https://www.readbyqxmd.com/read/28433000/elastic-moduli-of-electrospun-mats-importance-of-fiber-curvature-and-specimen-dimensions
#1
Vijay Kumar, Amit Rawal
Success of tissue engineering relies on the architecture and properties of porous scaffolds. Electrospun nonwoven scaffolds in the form of mats are unique materials due to large surface area to volume ratio, high porosity, versatility in surface functionalities and excellent mechanical properties. Maneuvering the mechanical behavior of the electrospun mat is a major challenge both from theoretical and experimental perspectives. Herein, we report a two-dimensional (2D) analytical model of normalized elastic moduli of electrospun mats by formulating a relationship with the governing fiber and structural parameters...
April 12, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28432818/characterization-of-decellularized-ovine-small-intestine-submucosal-layer-as-extracellular-matrix-based-scaffold-for-tissue-engineering
#2
Morteza Rashtbar, Jamshid Hadjati, Jafar Ai, Issa Jahanzad, Mahmoud Azami, Sadegh Shirian, Somayeh Ebrahimi-Barough, Esmaeil Sadroddiny
Extracellular matrix-based scaffolds derived from mammalian tissues have been used in tissue engineering applications. Among all the tissues, decellularized small intestine submucosal layer (SIS) has been recently investigated for its exceptional characteristics and biocompatibilities. These investigations have been mainly focused on the decellularized porcine SIS; however, there has not been any report on ovine SIS (OSIS) layer. In this study, OSIS was decellularized and its physical, chemical, and morphological properties were evaluated...
April 22, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28432001/in-vivo-and-in-vitro-study-of-osteogenic-potency-of-endothelin-1-on-bone-marrow-derived-mesenchymal-stem-cells
#3
Long-Wei Hu, Xiao Wang, Xin-Qun Jiang, Li-Qun Xu, Hong-Ya Pan
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration. Endothelin-1(EDN-1)is produced by vascular endothelial cells which plays an important role during bone development. However, its role in BMSCs remains largely unknown. We established EDN-1 overexpressed BMSCs, proliferation ability and osteogenesis differentiation were detected respectively...
April 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28431919/influence-of-scaffold-design-on-host-immune-and-stem-cell-responses
#4
REVIEW
Ashwin Nair, Liping Tang
The combined culture of isolated stem cells in tissue engineering scaffolds represents a popular strategy for the regeneration of specialized tissues. Despite of improved outcomes in some tissues, this stem cell-seeded tissue engineering strategy has not led to significant tissue regeneration as expected. The lower-than-expected outcome may be caused by overwhelming immune responses to scaffold materials and poor survival of seeded stem cells following implantation. This review is aimed at summarizing the success and failure of this strategy and also shedding some light on new directions to design scaffolds for promoting regenerative responses via autologous stem cells...
April 18, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28430301/towards-a-bioengineered-kidney-recellularization-strategies-for-decellularized-native-kidney-scaffolds
#5
Michele Fedecostante, Oana G Onciu, Koen G C Westphal, Rosalinde Masereeuw
Patients with end-stage renal disease often undergo dialysis as a partial substitute for kidney function while waiting for their only treatment option: a kidney transplant. Several research directions emerged for alternatives in support of the ever-growing numbers of patients. Recent years brought big steps forward in the field, with researchers questioning and improving the current dialysis devices as well as moving towards the design of a bioengineered kidney. Whole-organ engineering is also being explored as a possibility, making use of animal or human kidney scaffolds for engineering a transplantable organ...
April 20, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28430298/an-experimental-numerical-investigation-on-the-effects-of-macroporous-scaffold-geometry-on-cell-culture-parameters
#6
Hadis Eghbali, Michele M Nava, Gabriella Leonardi, Davod Mohebbi-Kalhori, Roberto Sebastiano, Abdolreza Samimi, Manuela T Raimondi
INTRODUCTION: Perfused bioreactors have been demonstrated to be effective in the delivery of nutrients and in the removal of waste products to and from the interior of cell-populated three-dimensional scaffolds. In this paper, a perfused bioreactor hosting a macroporous scaffold provided with a channel is used to investigate transport phenomena and culture parameters on cell growth. METHODS: MG63 human osteosarcoma cells were seeded on macroporous poly​(ε-caprolactone) scaffolds provided with a channel...
April 13, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28429412/bone-extracellular-matrix-hydrogel-enhances-osteogenic-differentiation-of-c2c12-myoblasts-and-mouse-primary-calvarial-cells
#7
Noura Alom, Heather Peto, Glen R Kirkham, Kevin M Shakesheff, Lisa J White
Hydrogel scaffolds derived from the extracellular matrix (ECM) of mammalian tissues have been successfully used to promote tissue repair in vitro and in vivo. The objective of this study was to evaluate the osteogenic potential of ECM hydrogels prepared from demineralized and decellularized bovine bone in the presence and absence of osteogenic medium. Culture of C2C12 and mouse primary calvarial cells (mPCs) on decellularized bone ECM (bECM) and demineralized bone matrix (DBM) gels resulted in increased expression of osteogenic gene markers, including a 3...
April 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28426898/improving-myoblast-differentiation-on-electrospun-poly-%C3%AE%C2%B5-caprolactone-scaffolds
#8
Phammela N Abarzúa-Illanes, Cristina Padilla, Andrea Ramos, Mauricio Isaacs, Jorge Ramos-Grez, Hugo C Olguín, Loreto M Valenzuela
Polymer scaffolds are used as an alternative to support tissue regeneration when it does not occur on its own. Cell response on polymer scaffolds is determined by factors such as polymer composition, topology, and the presence of other molecules. We evaluated the cellular response of murine skeletal muscle myoblasts on aligned or unaligned fibers obtained by electrospinning poly(ε-caprolactone) (PCL), and blends with poly(lactic-co-glycolic acid) (PLGA) or decorin, a proteoglycan known to regulate myogenesis...
April 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28426701/exploratory-study-on-the-effect-of-osteoactivin-on-muscle-regeneration-in-a-rat-volumetric-muscle-loss-model
#9
Jinjin Ma, Andrew R Baker, Anthony Calabro, Kathleen A Derwin
Wounds causing extensive injury loss of muscle, also known as volumetric muscle loss (VML), are frequently associated with high-energy civilian trauma and combat-related extremity injuries. Currently, no effective clinical therapy is available for promoting de novo muscle tissue regeneration to restore muscle function following VML. Recent studies have shown evidence that osteoactivin (OA), a transmembrane glycoprotein, has the ability to prevent skeletal muscle atrophy in response to denervation. Therefore the objective of this study is to investigate the potential regenerative effect of OA embedded and delivered via a cross-linked gelatin hydrogel within a volumetric tibialis anterior muscle defect in a rat model...
2017: PloS One
https://www.readbyqxmd.com/read/28425470/graphene-oxide-as-an-interface-phase-between-polyetheretherketone-and-hydroxyapatite-for-tissue-engineering-scaffolds
#10
Shuping Peng, Pei Feng, Ping Wu, Wei Huang, Youwen Yang, Wang Guo, Chengde Gao, Cijun Shuai
The poor bonding strength between biopolymer and bioceramic has remained an unsolved issue. In this study, graphene oxide (GO) was introduced as an interface phase to improve the interfacial bonding between polyetheretherketone (PEEK) and hydroxyapatite (HAP) for tissue engineering scaffolds. On the one hand, the conjugated structure of GO could form strong π-π stacking interaction with the benzene rings in PEEK. On the other hand, GO with a negatively charge resulting from oxygen functional groups could adsorb the positively charged calcium atoms (C sites) of HAP...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28424770/recent-advances-in-bioink-design-for-3d-bioprinting-of-tissues-and-organs
#11
REVIEW
Shen Ji, Murat Guvendiren
There is a growing demand for alternative fabrication approaches to develop tissues and organs as conventional techniques are not capable of fabricating constructs with required structural, mechanical, and biological complexity. 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of structure, mechanics, and biological matter [i.e., cells and extracellular matrix (ECM) components]. 3D bioprinting is an additive manufacturing approach that utilizes a "bioink" to fabricate devices and scaffolds in a layer-by-layer manner...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28423500/the-role-of-hesperetin-on-osteogenesis-of-human-mesenchymal-stem-cells-and-its-function-in-bone-regeneration
#12
Deting Xue, Erman Chen, Wei Zhang, Xiang Gao, Shengdong Wang, Qiang Zheng, Zhijun Pan, Hang Li, Ling Liu
Hesperetin has been suggested to be involved in bone strength. We aimed to investigate the effects of hesperetin on the osteogenic differentiation of human mesenchymal stem cells and its related mechanisms. We showed that hesperetin promoted osteogenic differentiation of human mesenchymal stem cells in vitro. It potentially exerts its effects via the ERK and Smad signaling pathways. Using a rat osteotomy model, we showed that human mesenchymal stem cells combined with a hesperetin/gelatin sponge scaffold resulted in accelerated fracture healing in vivo...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423354/cd54-rabbit-adipose-derived-stem-cells-overexpressing-hif-1%C3%AE-facilitate-vascularized-fat-flap-regeneration
#13
De-Quan Li, Guan-Ming Lu, Yi-Dan Liang, Zhi-Jie Liang, Min-Hong Huang, Qi-Liu Peng, Dong-Hua Zou, Rong-He Gu, Fang-Tian Xu, Hui Gao, Zhen-Dong Chen, Guang-Yi Chi, Zhong-Heng Wei, Li Chen, Hong-Mian Li
Fat flap transplantation is frequently performed in patients suffering from soft tissue defects resulting from disease or trauma. This study explored the feasibility of constructing vascularized fat flaps using rabbit adipose-derived stem cells (rASCs) and collagen scaffolds in a rabbit model. We evaluated rASCs proliferation, paracrine function, adipogenesis, vascularization, and CD54 expression, with or without HIF-1α transfection in vitro and in vivo. We observed that adipogenic differentiation potential was greater in rASCs with high CD54 expression (CD54+rASCs) than in those with low expression (CD54-rASCs), both in vitro and in vivo...
April 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422570/an-induced-adipocyte-sheet-reduces-inflammatory-reactions-during-remodeling-of-xenogeneic-scaffolds-in-vivo
#14
Tong Yi, Qiang Wang, Fan Fan, Gouhui Fan
Surgical therapy of cardiovascular diseases frequently requires replacement of diseased tissues with prosthetic devices or grafts. Calcification is the main reason for the degeneration of implanted grafts. However, some factors reduce stenosis and attenuate calcification of implanted grafts. In this study, we used an autologous induced adipocyte cell-sheet (IACS) as a drug delivery system to determine whether its secretion ability has a beneficial effect on the remodeling process of grafts in a rat subcutaneous model...
April 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28421875/three-dimensional-printing-and-its-applications-in-otorhinolaryngology-head-and-neck-surgery
#15
Trevor D Crafts, Susan E Ellsperman, Todd J Wannemuehler, Travis D Bellicchi, Taha Z Shipchandler, Avinash V Mantravadi
Objective Three-dimensional (3D)-printing technology is being employed in a variety of medical and surgical specialties to improve patient care and advance resident physician training. As the costs of implementing 3D printing have declined, the use of this technology has expanded, especially within surgical specialties. This article explores the types of 3D printing available, highlights the benefits and drawbacks of each methodology, provides examples of how 3D printing has been applied within the field of otolaryngology-head and neck surgery, discusses future innovations, and explores the financial impact of these advances...
November 1, 2016: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28420992/deficient-adipogenesis-of-scleroderma-patient-and-healthy-african-american-monocytes
#16
Rebecca Lee, Charles Reese, Gustavo Carmen-Lopez, Beth Perry, Michael Bonner, Marina Zemskova, Carole L Wilson, Kristi L Helke, Richard M Silver, Stanley Hoffman, Elena Tourkina
Monocytes from systemic sclerosis (SSc, scleroderma) patients and healthy African Americans (AA) are deficient in the regulatory protein caveolin-1 leading to enhanced migration toward chemokines and fibrogenic differentiation. While dermal fibrosis is the hallmark of SSc, loss of subcutaneous adipose tissue is a lesser-known feature. To better understand the etiology of SSc and the predisposition of AA to SSc, we studied the adipogenic potential of SSc and healthy AA monocytes. The ability of SSc and healthy AA monocytes to differentiate into adipocyte-like cells (ALC) is inhibited compared to healthy Caucasian (C) monocytes...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28420433/umbilical-cord-derived-mesenchymal-stem-cells-on-scaffolds-facilitate-collagen-degradation-via-upregulation-of-mmp-9-in-rat-uterine-scars
#17
Lu Xu, Lijun Ding, Lei Wang, Yun Cao, Hui Zhu, Jingjie Lu, Xin'an Li, Tianran Song, Yali Hu, Jianwu Dai
BACKGROUND: Severe injuries of the uterus may trigger uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat uterine scars following full-thickness excision of uterine walls...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28420432/elevated-gab2-induces-tumor-growth-and-angiogenesis-in-colorectal-cancer-through-upregulating-vegf-levels
#18
Chenbo Ding, Junmin Luo, Xiaobo Fan, Longmei Li, Shanshan Li, Kunming Wen, Jihong Feng, Guoqiu Wu
BACKGROUND: Grb2-associated binder 2 (Gab2) is a scaffolding protein that serves as a critical signaling amplifier downstream of tyrosine kinase receptors. Our previous study has shown that Gab2 induces epithelial-to-mesenchymal transition (EMT) and promotes metastasis in colorectal cancer (CRC). However, the role of Gab2 in CRC growth and angiogenesis remains unclear. METHODS: The expression of vascular endothelial growth factor (VEGF) in different colorectal tissues was detected by immunohistochemistry and qRT-PCR to evaluate its correlation with Gab2...
April 18, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28420296/the-effect-of-engineered-nanotopography-of-electrospun-microfibers-on-fiber-rigidity-and-macrophage-cytokine-production
#19
Nicholas J Schaub, Anthony R D'Amato, Andrew Mason, David T Corr, Erin Y Harmon, Michelle R Lennartz, Ryan J Gilbert
Currently, it is unknown how the mechanical properties of electrospun fibers, and the presentation of surface nanotopography influence macrophage gene expression and protein production. By further elucidating how specific fiber properties (mechanical properties or surface properties) alter macrophage behavior, it may be possible to create electrospun fiber scaffolds capable of initiating unique cellular and tissue responses. In this study, we determined the elastic modulus and rigidity of fibers with varying topographies created by finely controlling humidity and including a non-solvent during electrospinning...
April 18, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28420291/a-study-of-long-term-stability-and-antimicrobial-activity-of-chlorhexidine-polyhexamethylene-biguanide-and-silver-nanoparticle-incorporated-in-sericin-based-wound-dressing
#20
Sumate Ampawong, Pornanong Aramwit
In this study, three kinds of antiseptics which were 0.05% chlorhexidine, 0.2% polyhexamethylene biguanide (PHMB), or 200 ppm silver nanoparticle was introduced to incorporate in the sericin-based scaffold to produce the antimicrobial dressing for the treatment of infected chronic wound. The effects of antiseptic incorporation on the stability, release of sericin, and short-term and long-term (6 months) antimicrobial activity of the sericin dressing against gram-negative and gram-positive bacteria were investigated...
April 19, 2017: Journal of Biomaterials Science. Polymer Edition
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