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https://www.readbyqxmd.com/read/28930993/confocal-imaging-of-neuropeptide-y-phluorin-a-technique-to-visualize-insulin-granule-exocytosis-in-intact-murine-and-human-islets
#1
Madina Makhmutova, Tao Liang, Herbert Gaisano, Alejandro Caicedo, Joana Almaça
Insulin secretion plays a central role in glucose homeostasis under normal physiological conditions as well as in disease. Current approaches to study insulin granule exocytosis either use electrophysiology or microscopy coupled to the expression of fluorescent reporters. However most of these techniques have been optimized for clonal cell lines or require dissociating pancreatic islets. In contrast, the method presented here allows for real time visualization of insulin granule exocytosis in intact pancreatic islets...
September 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28925709/effect-of-hydrophobic-chain-length-on-the-stability-and-guest-exchange-behavior-of-shell-sheddable-micelles-formed-by-disulfide-linked-diblock-copolymers
#2
Haiyan Fan, Yixia Li, Jinxian Yang, Xiaodong Ye
Reduction-responsive micelles hold enormous promise for the application as drug carriers due to the fast drug release triggered by reducing conditions and high anticancer activity. However, the effect of hydrophobic chain length on the stability and guest exchange of reduction-responsive micelles, especially for the micelles formed by diblock copolymers containing single disulfide group, are not fully understood. Here, shell-sheddable micelles formed by a series of disulfide-linked copolymer poly(ethylene glycol)-b-poly(-caprolactone) (PEG-SS-PCL) containing the same chain length of PEG but different chain lengths of hydrophobic block PCL were prepared and well characterized...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28925589/recurrent-twin-twin-transfusion-syndrome-rttts-and-twin-anemia-polycythemia-sequence-taps-after-fetoscopic-laser-surgery-fls-size-of-the-cannula-does-matter
#3
Roopali Donepudi, Joost Akkermans, Lovepreet Mann, Frans J Klumper, Johanna M Middeldorp, Enrico Lopriore, Kenneth J Moise, Michael Bebbington, Anthony Johnson, Dick Oepkes, Ramesha Papanna
OBJECTIVES: Optimal outcomes after fetoscopic laser surgery (FLS) for twin-twin transfusion (TTTS) depend on the successful completion of ablation of all placental anastomoses. Our objective was to determine the incidence and risk factors for the occurrence of recurrent TTTS (rTTTS) or twin anemia-polycythemia sequence (TAPS) after FLS, and focusing on the impact of different diameters of the cannula. METHODS: A secondary analysis of prospectively collected data of 666 consecutive patients undergoing laser surgery for TTTS from 2 centers was performed...
September 19, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28917946/cellular-uptake-and-intracellular-localization-of-poly-acrylic-acid-nanoparticles-in-a-rainbow-trout-oncorhynchus-mykiss-gill-epithelial-cell-line-rtgill-w1
#4
Lindsey C Felix, Van A Ortega, Greg G Goss
The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of NP-containing products down the drain and/or in landfills, as well as direct usage on agricultural land. Therefore, NPs will inevitably contaminate aquatic environments and interact with resident organisms...
September 7, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28917730/direct-and-comprehensive-analysis-of-dyes-based-on-integrated-molecular-and-structural-information-via-laser-desorption-laser-postionization-mass-spectrometry
#5
Rong Liu, Zhibin Yin, Yixin Leng, Wei Hang, Benli Huang
Laser desorption laser postionization time-of-flight mass spectrometry (LDPI-TOFMS) was employed for direct analysis and determination of typical basic dyes. It was also used for the analysis and comprehensive understanding of complex materials such as blue ballpoint pen inks. Simultaneous emergences of fragmental and molecular information largely simplify and facilitate unambiguous identification of dyes via variable energy of 266nm postionization laser. More specifically, by optimizing postionization laser energy with the same energy of desorption laser, the structurally significant results show definite differences in the fragmentation patterns, which offer opportunities for discrimination of isomeric species with identical molecular weight...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28914045/electron-transfer-from-bi-isonicotinic-acid-emerges-upon-photodegradation-of-n3-sensitized-tio2-electrodes
#6
Melike Karakus, Wen Zhang, Hans Joachim Räder, Mischa Bonn, Enrique Cánovas
The long-term stability of dye sensitized solar cells (DSSCs) is determined to a large extent by the photodegradation of their sensitizers. Understanding the mechanism of light induced decomposition of dyes sensitizing a mesoporous oxide matrix may therefore contribute to solutions to increase the life span of DSSCs. Here, we investigate, using ultrafast terahertz photoconductivity measurements, the evolution of interfacial electron transfer (ET) dynamics in N3 dye-sensitized mesoporous TiO2 electrodes upon dye photodegradation...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28905964/development-and-applications-of-a-near-infrared-dye-benzylguanine-conjugate-to-specifically-label-snap-tagged-proteins
#7
Xinbo Song, Hui Bian, Chao Wang, Mingyu Hu, Ning Li, Yi Xiao
Near-infrared (NIR) fluorescent probes are advantageous over visible ones, for they can avoid the interference from the short-wavelength background emission in biological systems. However, there are a very limited number of NIR probes that can specifically label target proteins in living cells. In this work, a series of long-wavelength dyes (N-NIR, S-NIR, and K-NIR) analogous to the novel Changsha NIR family are synthesized conveniently through a new approach that is different from the previously reported one...
September 14, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28902984/fine-and-clean-photothermally-controlled-nir-drug-delivery-from-biocompatible-nickel-bis-dithiolene-containing-liposomes
#8
Kenny Mebrouk, Mathieu Ciancone, Thomas Vives, Sandrine Cammas-Marion, Thierry Benvegnu, Catherine Le Goff-Gaillard, Yannick Arlot-Bonnemains, Marc Fourmigué, Franck Camerel
This work demonstrates that metal-bis(dithiolene) complexes can be efficiently incorporated inside organic nanocarriers and, that under near-infrared (NIR) irradiation, their high photothermal activity can be finely used to release encapsulated drugs on demand. In contrast to gold nanoparticles and other organic NIR dyes, nickel-bis(dithiolene) complexes do not produce singlet oxygen under irradiation, a highly desirable characteristic to preserve the chemical integrity and activity of the loaded drug during the NIR-triggered release from the nanocarriers...
September 13, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28892383/o2-b-1-%C3%AE-g-quenching-by-o2-co2-h2o-and-n2-at-temperatures-of-300-800-k
#9
Marsel Vakifovich Zagidullin, Nikolay Anatol'evich Khvatov, Iakov A Medvedkov, Georgy I Tolstov, Alexander Moiseevich Mebel, Michael Charles Heaven, Valeriy N Azyazov
Rate constants for the removal of O2 b1Σg+ by collisions with O2, N2, CO2 and H2O have been determined over the temperature range from 297 to 800 K. O2(b1Σg+) was excited by pulses from a tunable dye laser, and the deactivation kinetics were followed by observing the temporal behavior of the b1Σg+ - X3Σg- fluorescence. The removal rate constants for CO2, N2 and H2O were not strongly dependent on temperature, and could be represented by the expressions kCO2=(1.18±0.05)×10-17×T^1.5×exp((595 ± 25) /T); kN2=(8±0...
September 11, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28884917/molluscum-contagiosum-review-and-update-on-management
#10
REVIEW
Emily Forbat, Firas Al-Niaimi, Faisal R Ali
Molluscum contagiosum (MC) is an infectious dermatosis that commonly presents in children and immunocompromised individuals. Although lesions usually resolve spontaneously after several months, they can be symptomatic and cause psychosocial distress. We review the evidence underlying treatment methods available for MC lesions, including potassium hydroxide, salicylic acid, hydrogen peroxide, retinoids, cantharidin, cryotherapy, curettage, and pulsed dye laser to aid practicing dermatologists in therapy selection...
September 2017: Pediatric Dermatology
https://www.readbyqxmd.com/read/28883632/extraction-separation-and-characterization-of-endotoxins-in-water-samples-using-solid-phase-extraction-and-capillary-electrophoresis-laser-induced-fluorescence
#11
Fun Man Fung, Min Su, Hua-Tao Feng, Sam Fong Yau Li
This study focuses on one of the key environmental threats, endotoxins, also known as lipopolysaccharides (LPS). A capillary electrophoresis method in combination with laser induced fluorescence (LIF) detection was developed for the analysis of endotoxins from 16 different bacterial strains. LPSs were derivatized with the amino-reactive fluorescent dye, fluorescein isothiocyanate (FITC), separated by capillary zone electrophoresis (CZE) under the optimized conditions with the use of 50 mM sodium tetraborate buffer (pH 9...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28875100/development-of-an-advanced-diagnostic-concept-for-intestinal-inflammation-molecular-visualisation-of-nitric-oxide-in-macrophages-by-functional-poly-lactic-co-glycolic-acid-microspheres
#12
Kathleen Lange, Christian Lautenschläger, Maria Wallert, Stefan Lorkowski, Andreas Stallmach, Alexander Schiller
We here describe a new approach to visualise nitric oxide (NO) in living macrophages by fluorescent NO-sensitive microspheres based on poly(lactic-co-glycolic acid) (PLGA). PLGA microspheres loaded with NO550 dye were prepared through a modified solvent-evaporation method. Microparticles were characterized by a mean hydrodynamic diameter of 3000 nm, zeta potential of -26.000 ± 0.351 mV and a PDI of 0.828 ± 0.298. Under abiotic conditions, NO release was triggered through UV radiation (254 nm) of 10 mM sodium nitroprusside dehydrate (SNP)...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28858925/pulsed-dye-laser-at-subpurpuric-settings-for-the-treatment-of-pulsed-dye-laser-induced-ecchymoses-in-patients-with-port-wine-stains
#13
Jeremy A Brauer, Joshua A Farhadian, Leonard J Bernstein, Yoon-Soo Bae, Roy G Geronemus
BACKGROUND: Post-procedure purpura is a major complaint of patients with port-wine stains (PWSs) treated with pulsed dye laser (PDL). OBJECTIVE: To assess the safety and efficacy of using PDL at nonpurpuric settings to treat ecchymoses that develop within PWSs after treatment with PDL. MATERIALS AND METHODS: Prospective, randomized, controlled study using 595-nm PDL for treatment of PWSs and laser-induced ecchymoses. Port-wine stains were treated in entirety at baseline...
August 30, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28852292/fractional-carbon-dioxide-long-pulse-nd-yag-and-pulsed-dye-laser-in-the-management-of-keloids
#14
Ashwini Annabathula, C Shanmuga Sekar, C R Srinivas
BACKGROUND: Keloids are abnormal wound responses characterised by excessive deposition of collagen and glycoprotein. They are both aesthetically and symptomatically distressing for most of the patients. There are reports of keloid management with pulsed dye laser (PDL), fractional carbon dioxide (CO2) laser and neodymium-doped yttrium aluminium garnet (Nd:YAG) laser individually and also in combination of CO2 with PDL and CO2 with Nd:YAG. Here, we discuss a combination of all the 3 lasers as a therapy for keloids...
April 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28842769/time-of-flight-ion-mobility-spectrometry-in-combination-with-laser-induced-fluorescence-detection-system
#15
Florian Uteschil, Andriy Kuklya, Klaus Kerpen, Robert Marks, Ursula Telgheder
A laser-induced fluorescence (LIF) was used as a complimentary detection system for time-of-flight ion mobility spectrometry (TOF-IMS). A LIF detection system is potentially faster than a conventional electrometer detector and can provide additional (to usual for IMS drift time) analytical information, namely wavelength of fluorescence maxima and fluorescence lifetime. Therefore, better discrimination ability can be expected. Additionally, the combination of IMS and LIF operates at atmospheric pressure. This allows fluorescence measurements of specified ions and ion clusters, which would not survive in a mass spectrometer...
August 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28841992/laser-carved-micro-crack-channels-in-paper-based-dilution-devices
#16
Qian Liu, Chaoping Xu, Heng Liang
We developed novel laser carved micro-crack (LCC) paper-based channels to significantly accelerate the liquid flow without an external pump. For the aqueous solutions they increased the flow velocity 59 times in 16% laser power-8 micro-cracks-LCC channel compared with it in solely-printed channels. All experimental data from both LCC and solely-printed channels were well-fitted by the time-distance quadratic trinomial that we developed on laser power and micro-crack number. We designed and fabricated T-junction microstructures of LCCs...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28841139/sensitive-optical-bio-sensing-of-p-type-wse2-hybridized-with-fluorescent-dye-attached-dna-by-doping-and-de-doping-effects
#17
Kyu Hyun Han, Jun Young Kim, Seong Gi Jo, Changwon Seo, Jeongyong Kim, Jinsoo Joo
Layered transition metal dichalcogenides (TMDCs), such as MoS<sub>2</sub>, WSe<sub>2</sub>, and WS<sub>2</sub>, are exciting two-dimensional (2-D) materials because they possess tunable optical and electrical properties that depend on the number of layers. In this study, the nanoscale photoluminescence (PL) characteristics of the p-type WSe<sub>2</sub> monolayer, and WSe<sub>2</sub> layers hybridized with the fluorescent dye Cy3 attached to probe DNA (Cy3/p-DNA), have been investigated as a function of the concentration of Cy3/DNA by using high-resolution laser confocal microscopy (LCM)...
August 25, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28839420/comparison-of-three-different-sealer-placement-techniques-an-in-vitro-confocal-laser-microscopic-study
#18
Avoy Kumar Dash, Shanin Farista, Abhilasha Dash, Ajinkya Bendre, Sana Farista
INTRODUCTION: Three-dimensional obturation of the root canal system is the final objective of root canal therapy. Greater penetration of sealer in root dentine lesser will be the voids at the dentine-sealer interface. Hence, analysis of the dentin/sealer interface allows the determination of a filling technique which could obturate the root canals with least gaps and voids. Therefore, the aim of this study is to compare the depth and percentage of sealer penetration into root dentin using three different root canal sealer placement techniques under confocal laser scanning microscope...
April 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28835908/photothrombotic-induction-of-capillary-ischemia-in-the-mouse-cortex-during-in-vivo-two-photon-imaging
#19
Robert G Underly, Andy Y Shih
Photothrombosis of blood vessels refers to the activation of a circulating photosensitive dye with a green light to induce clotting in vivo (Watson et al., 1985). Previous studies have described how a focused green laser could be used to noninvasively occlude pial arterioles and venules at the brain surface (Schaffer et al., 2006; Nishimura et al., 2007; Shih et al., 2013). Here we show that small regions of the capillary bed can similarly be occluded to study the ischemic response within the capillary system of the mouse cerebral cortex...
July 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28831254/development-of-a-novel-microemulsion-for-oral-absorption-enhancement-of-all-trans-retinoic-acid
#20
Thirapit Subongkot, Tanasait Ngawhirunpat
This study was aimed to develop a novel microemulsion that contained oleth-5 as a surfactant to enhance the oral absorption of all-trans retinoic acid (ATRA). The prepared microemulsion was evaluated for its particle size, shape, zeta potential, in vitro release, in vitro intestinal absorption, intestinal membrane cytotoxicity and stability. The obtained microemulsion was spherical in shape with a particle size of <200 nm and a negative surface charge. The in vitro release of the ATRA-loaded microemulsion was best fit with the zero-order model...
2017: International Journal of Nanomedicine
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