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Dye laser

Matthew Essandoh, Rafael A Garcia
Magnetic particles entrapped in different matrices that display high thermal stability, low toxicity, interactive functions at the surface, and high saturation magnetization are of great interest. The objective of this work was to synthesize a novel hemoglobin/iron oxide composite (Hb/Fe3 O4 ) for the removal of different dyes (indigo carmine, naphthol blue black, tartrazine, erythrosine, eriochrome black T and bromophenol blue) from aqueous solutions. The Hb/Fe3 O4 composite was characterized using scanning electron microscopy (SEM), laser diffraction particle size analysis, FT-IR spectroscopy, isoelectric point determination and thermogravimetric analysis (TGA)...
May 10, 2018: Chemosphere
Wei Peng, Henriette S de Bruijn, Eric Farrell, Mouldy Sioud, Vida Mashayekhi, Sabrina Oliveira, Go M van Dam, Jan L N Roodenburg, Max J H Witjes, Dominic J Robinson
OBJECTIVE: The aim of this study was to investigate the effects of targeted photoimmunotherapy (PIT) in vitro on cell lines with various expression levels of epidermal growth factor receptor (EGFR) using an anti-EGFR targeted conjugate composed of Cetuximab and IR700DX, phthalocyanine dye. MATERIALS AND METHODS: Relative EGFR density and cell binding assay was conducted in three human head & neck cancer cell lines (scc-U2, scc-U8, and OSC19) and one reference cell line A431...
May 19, 2018: Lasers in Surgery and Medicine
Minjeong Gu, Keunchang Cho, Seong Ho Kang
The migration behavior of organic fluorescent dyes (i.e., crystal violet, methyl violet base, methyl violet B base, rhodamine 6G, and rhodamine B base) in non-aqueous capillary electrophoresis (NACE) was investigated by focusing on the physicochemical properties of various organic solvents [ethanol, methanol, 2-propanol, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO)] in background electrolyte (BGE). Laser-induced fluorescence (LIF) and UV/Vis detectors were employed to observe both the migration time of organic dyes and the electroosmotic flow (EOF) in NACE, respectively...
May 15, 2018: Journal of Chromatography. A
Wen Liu, Yalun Wang, Xiao Han, Ping Lu, Liang Zhu, Chaowei Sun, Jun Qian, Sailing He
Near-infrared (NIR) fluorescence is very important for high-contrast biological imaging of high-scattering tissues such as brain tissue. Unfortunately, commercial NIR dyes are excited usually by visible light, and their multi-photon absorption (MPA) cross-sections are small. Here, we design new co-encapsulated NIR nanoparticles (NPs) with a large three-photon (3PA) absorption cross-section. A form of aggregation-induced emission (AIE) luminogen (AIEgen), 2,3-bis(4'-(diphenylamino)-[1,1'-biphenyl]-4-yl) fumaronitrile (TPATCN), is introduced as the donor, and a form of NIR dye, silicon 2,3-naphthalocyanine bis-(trihexylsilyloxide) (NIR775), is adopted as the acceptor...
May 18, 2018: Nanoscale
Joshua T Maxwell, Chin-Hsien Tsai, Tahmina A Mohiuddin, Jennifer Q Kwong
Ca2+ handling by mitochondria is a critical function regulating both physiological and pathophysiological processes in a broad spectrum of cells. The ability to accurately measure the influx and efflux of Ca2+ from mitochondria is important for determining the role of mitochondrial Ca2+ handling in these processes. In this report, we present two methods for the measurement of mitochondrial Ca2+ handling in both isolated mitochondria and cultured cells. We first detail a plate reader-based platform for measuring mitochondrial Ca2+ uptake using the Ca2+ sensitive dye calcium green-5N...
April 27, 2018: Journal of Visualized Experiments: JoVE
Abeer N Al-Romaizan, Mahmoud A Hussein
A new candidate laser dye based 1,4-bis[β-(2-naphthothisolyl) vinyl] benzene (BNTVB) were prepared, and characterized in various organic solvents. The center polarity is less sensitive than electronic absorption. A red shift was noticed in the fluorescence spectra (ca. 40 nm) with increment in the solvent's polarity, this means that BNTVB's polarity appreciates upon excitation. The dipole moment of ground state (μg ) and the excited singlet state dipole moment (μe ) are determined from Kawski - Chamma and Bakshiev-Viallet equations using the disparity of Stokes shift with solvent polarity function of ε (dielectric constant) and n (refractive index) of the solvent...
May 12, 2018: Journal of Fluorescence
Jiafang Zhu, Wenxin Yu, Tianyou Wang, Yijie Chen, Dongze Lyu, Lei Chang, Gang Ma, Xiaoxi Lin
Port-wine stains (PWS) affect 0.3 to 0.5% of newborns and pulsed dye laser (PDL) remains the treatment of choice. However, no reliable study regarding the benefits of more frequent has been conducted. We designed the present study to evaluate whether more frequent PDL treatments in infantile patients would achieve further lightening of erythema. We prospectively investigated 20 infants with PWS. Two adjacent sites were both treated for a 12-week duration and randomly allocated to be treated for seven sessions at 2-week intervals or three sessions at 6-week intervals...
May 10, 2018: Lasers in Medical Science
Shuaidi Zhang, Rui Xiong, Mahmoud A Mahmoud, Elizabeth Quigley, Huibin Chang, Mostafa A El-Sayed, Vladimir V Tsukruk
We demonstrate that cellulose nanofiber (CNF) biomaterials with high transparency and mechanical robustness can be combined with gold nanorods to form a multifunctional porous membrane for dual-mode surface enhanced Raman scattering detection of both small molecules and cells. The nanoporous nature of the plasmonic nanofiber membranes allows for effective molecular filtration and pre-concentration of the analytes, further boosting the SERS performance. Specifically, due to the low fluorescence and Raman background of the CNF matrix, extremely low loading density of gold nanorods can be used...
May 8, 2018: ACS Applied Materials & Interfaces
Zainab Laftah, Firas Al-Niaimi
Xanthelasmas are localized accumulation of lipid deposits on the eyelids. Lesions are typically asymptomatic and treatment is often sought for cosmetic purposes. Unfortunately, there is paucity of strong evidence in the literature for the effective treatment of normolipidemic xanthelasmas. A literature search using the term "xanthelasma" was carried out in PubMed and Medline databases. Only articles related to treatment were considered and analyzed for their data. Commonly cited treatments include topical trichloroacetic acid, liquid nitrogen cryotherapy, and various lasers including carbon dioxide, Er:YAG, Q-switched Nd:YAG, and pulse dye laser...
January 2018: Journal of Cutaneous and Aesthetic Surgery
Kazuki Hotta, Bradley Jon Behnke, Kazuto Masamoto, Rie Shimotsu, Naoya Onodera, Akihiko Yamaguchi, David C Poole, Yutaka Kano
Via modulation of endothelial integrity and vascular permeability in response to damage skeletal muscle microvessels play a crucial permissive role in tissue leukocyte invasion. However, direct visual evidence of altered microvascular permeability of skeletal muscle has not been technically feasible impairing mechanistic understanding of these responses. Two-photon laser scanning microscopy (TPLSM) allows three-dimensional in vivo imaging of skeletal muscle microcirculation. We hypothesized that the regulation of microvessels permeability in vivo is temporally related to acute inflammatory and regenerative processes following muscle injury...
May 3, 2018: Journal of Applied Physiology
J Madsen, R E Ducker, O Al Jaf, M L Cartron, A M Alswieleh, C H Smith, C N Hunter, S P Armes, G J Leggett
Binary brush structures consisting of poly(cysteine methacrylate) (PCysMA) "corrals" enclosed within poly(oligoethylene glycol methyl ether methacrylate) (POEGMA) "walls" are fabricated simply and efficiently using a two-step photochemical process. First, the C-Cl bonds of 4-(chloromethyl)phenylsilane monolayers are selectively converted into carboxylic acid groups by patterned exposure to UV light through a mask and POEGMA is grown from unmodified chlorinated regions by surface-initiated atom-transfer radical polymerisation (ATRP)...
February 28, 2018: Chemical Science
Han Zhang, Yuze Sun
We present an optofluidic droplet dye laser that is generated by an array of microfluidic nozzles fabricated on a polycarbonate chip. A droplet resonator forms upon pressurizing the nozzle backside microfluidic channel. Multimode low-threshold lasing is observed from individual microdroplets doped with dye. Additionally, droplets can be conveniently released from the nozzle by water rinsing from the top microfluidic channel and subsequently regenerated, and thus achieve optofluidic lasers on-demand. Our work demonstrates a new approach to generating on-chip laser source and laser arrays in a simple, reproducible, reconfigurable, and low-cost fashion...
April 30, 2018: Optics Express
Chih-Hao Liu, Dmitry Nevozhay, Alexander Schill, Manmohan Singh, Susobhan Das, Achuth Nair, Zhaolong Han, Salavat Aglyamov, Kirill V Larin, Konstantin V Sokolov
Wave-based optical elastography is rapidly emerging as a powerful technique for quantifying tissue biomechanical properties due to its noninvasive nature and high displacement sensitivity. However, current approaches are limited in their ability to produce high-frequency waves and highly localized mechanical stress. In this Letter, we demonstrate that the rapid liquid-to-gas phase transition of dye-loaded perfluorocarbon nanodroplets ("nanobombs") initiated by a pulsed laser can produce highly localized, high-frequency, and broadband elastic waves...
May 1, 2018: Optics Letters
Andy Ying Guey Fuh, Ming-Hsien Li, Te-Wei Chang, Yu-I Lee, Shing Trong Wu
This study demonstrates the optical manipulation of colloids dispersed in azo-dye-doped liquid crystals (DDLCs) where the accumulation occurs at the interfaces of the phase domains. We explain the mechanism related to the formation of the domains and the movement of the colloids in DDLCs with respect to the isomerization of azo-dye molecules via the illumination of laser beams. The colloids are dragged to the interfaces of the isotropic/nematic domain and the air bubble/isotropic domain by molecular interaction and Marangoni flows...
April 20, 2018: Applied Optics
Alexander Durst, Michael Wensing, Edouard Berrocal
This paper describes the adaptation of the laser-induced fluorescence measurement technique for the investigation of the primary breakup of modern diesel and gasoline direct injection sprays. To investigate the primary breakup, a microscopic technique is required, and with the help of special tracer dyes, a high fluorescence signal can be achieved in the visible range of the electromagnetic spectrum, resulting in good image quality with a nonintensified camera. Besides the optimization of the optical setup for the microscopic field of view, different tracer dyes are compared, and their solubility and fluorescence are tested in the desired surrogate and real-world fuels...
April 1, 2018: Applied Optics
Md Rejvi Kaysir, Simon Fleming, Alexander Argyros
Luminescent solar concentrators (LSCs) utilizing stimulated emission by a seed laser are a promising approach to overcome the limitations of conventional LSCs, with a significant reduction of the photovoltaic material. In our previous work, we demonstrated the principle of a stimulated LSC (s-LSC) and correspondingly developed a model for quantifying the output power of such a system, taking into account different important physical parameters. The model suggested Perylene Red (PR) dye as a potential candidate for s-LSCs...
April 1, 2018: Applied Optics
Nicole M Wakida, Gladys Mae S Cruz, Clarissa C Ro, Emmanuel G Moncada, Nima Khatibzadeh, Lisa A Flanagan, Michael W Berns
This study aims to understand the phagocytic response of astrocytes to the injury of neurons or other astrocytes at the single cell level. Laser nanosurgery was used to damage individual cells in both primary mouse cortical astrocytes and an established astrocyte cell line. In both cases, the release of material/substances from laser-irradiated astrocytes or neurons induced a phagocytic response in near-by astrocytes. Propidium iodide stained DNA originating from irradiated cells was visible in vesicles of neighboring cells, confirming phagocytosis of material from damaged cortical cells...
2018: PloS One
Santosh Lohumi, Hoonsoo Lee, Moon S Kim, Jianwei Qin, Lalit Mohan Kandpal, Hyungjin Bae, Anisur Rahman, Byoung-Kwan Cho
The potential adulteration of foodstuffs has led to increasing concern regarding food safety and security, in particular for powdered food products where cheap ground materials or hazardous chemicals can be added to increase the quantity of powder or to obtain the desired aesthetic quality. Due to the resulting potential health threat to consumers, the development of a fast, label-free, and non-invasive technique for the detection of adulteration over a wide range of food products is necessary. We therefore report the development of a rapid Raman hyperspectral imaging technique for the detection of food adulteration and for authenticity analysis...
2018: PloS One
M Paula Militello, Marcela S Altamirano, Sonia G Bertolotti, Carlos M Previtali
The interaction of the singlet and triplet excited states of the synthetic dye safranine-O with carboxyl-terminated poly(amidoamine) (PAMAM) dendrimers was investigated in a buffer solution at pH 8. Low half-generation PAMAM dendrimers (G -0.5; G +0.5: G 1.5) were employed. The UV-vis absorption spectrum of the dye presents only a very small red shift in the presence of dendrimers. Fluorescence quenching was detected and it was interpreted by a static mechanism in terms of the association of the dye with the dendrimer...
April 30, 2018: Photochemical & Photobiological Sciences
Li Zhang, Jie Peng, Ming-Fang Hong, Jia-Qing Chen, Ru-Ping Liang, Jian-Ding Qiu
A facile and sensitive method for the quantitative detection of telomerase and in situ imaging of intracellular telomerase is developed by using a graphene oxide (GO)-based fluorescent nanosensor. The nanosensor consists of a fluorescent DNA (P1) adsorbed on the GO surface. Here, GO serves not only as a fluorescence quencher but also as a carrier to successfully transport P1 into cancer cells as a signal reporter. P1 is a dye-labeled single-stranded DNA complementary to the telomeric repeated sequence, and initially the combination of P1 and GO exhibits minimal background fluorescence...
April 30, 2018: Analyst
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