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https://www.readbyqxmd.com/read/28445714/the-toll-like-receptor-2-agonist-pam3csk4-is-neuroprotective-after-spinal-cord-injury
#1
Nicole S Stivers, Nicolas Pelisch, Ben C Orem, Joshua Williams, Jacqueline M Nally, David P Stirling
Microglia/macrophage activation and recruitment following spinal cord injury (SCI) is associated with both detrimental and reparative functions. Stimulation of the innate immune receptor Toll-like receptor-2 (TLR2) has shown to be beneficial following SCI, and it increases axonal regeneration following optic nerve crush. However, the mechanism(s) remain unclear. As microglia express high levels of TLR2, we hypothesized that modulating the microglial response to injury using a specific TLR2 agonist, Pam3CSK4, would prevent secondary-mediated white matter degeneration following SCI...
April 23, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28443303/cutaneous-ulceration-and-breast-implant-compromise-after-pulse-dye-laser-for-radiation-induced-telangiectasias
#2
Anthony M Rossi, Kishwer S Nehal, Babak Mehrara, Erica Lee
No abstract text is available yet for this article.
May 2017: JAAD Case Reports
https://www.readbyqxmd.com/read/28442407/light-magnetic-hyperthermia-triggered-drug-released-from-multi-functional-thermo-sensitive-magnetoliposomes-for-precise-cancer-synergetic-theranostics
#3
Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Precise delivery of antineoplastic drugs to specific tumor region has drawn much attention in recent years. Herein, a light/magnetic hyperthermia triggered drug delivery with multiple functionality is designed based on methotrexate (MTX) modified thermo-sensitive magnetoliposomes (MTX-MagTSLs). In this system, MTX and oleic acid modified magnetic nanoparticles (MNPs) can be applied in biological and magnetic targeting. Meanwhile, lipophilic fluorescent dye Cy5.5 and MNPs are encapsulated into the bilayer of liposomes, which can not only achieve dual-imaging effect to verify the MTX-MagTSLs accumulation in tumor region, but also provide an appropriate laser irradiation region to release Doxorubicin (Dox) under alternating magnetic field (AMF)...
April 22, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28439823/high-throughput-rt-qpcr-for-the-analysis-of-circulating-micrornas
#4
Geok Wee Tan, Lu Ping Tan
Reverse transcription followed by real-time or quantitative polymerase chain reaction (RT-qPCR) is the gold standard for validation of results from transcriptomic profiling studies such as microarray and RNA sequencing. The current need for most studies, especially biomarker studies, is to evaluate the expression levels or fold changes of many transcripts in a large number of samples. With conventional low to medium throughput qPCR platforms, many qPCR plates would have to be run and a significant amount of RNA input per sample will be required to complete the experiments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28439814/imaging-the-accumulated-intracellular-microalgal-lipids-as-a-response-to-temperature-stress
#5
Khaled N M Elsayed, Tatiana A Kolesnikova, Anja Noke, Gerd Klöck
Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28426977/blue-violet-laser-inactivates-methicillin-resistant-staphylococcus-aureus-by-altering-its-transmembrane-potential
#6
Gabriel Biener, Daniela S Masson-Meyers, Violet V Bumah, Grant Hussey, Michael R Stoneman, Chukuka S Enwemeka, Valerica Raicu
The resistance of methicillin-resistant Staphylococcus aureus to antibiotics presents serious clinical problems that prompted the need for finding alternative or combination therapies. One such therapy is irradiation with blue light. To determine the alterations in metabolic processes implicated in the observed antimicrobial effects of blue light, we investigated the changes in membrane potential and the presence of free-radical-producing photo-acceptor molecules. Bacterial cultures irradiated with one or two doses of 405nm laser light (each consisting of 121J/cm(2)) were imaged with spectrally resolved laser-scanning microscopes to detect endogenous fluorescent species as well as the voltage sensitive dye 3,3'-Diethyloxacarbocyanine iodide...
April 6, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28426178/multiplexing-n-glycan-analysis-by-dna-analyzer
#7
Hua-Tao Feng, Pingjing Li, Guo Rui, James Stray, Shaheer Khan, Shiaw-Min Chen, Sam F Y Li
Analysis of N-glycan structures has been gaining attentions over the years due to their critical importance to biopharma based applications and growing roles in biological research. Glycan profiling is also critical to the development of biosimilar drugs. The detailed characterization of N-glycosylation is mandatory because it is a non-template driven process and that significantly influences critical properties such as bio-safety and bio-activity. The ability to comprehensively characterize highly complex mixtures of N-glycans has been analytically challenging and stimulating because of the difficulties in both the structure complexity and time-consuming sample pretreatment procedures...
April 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28425593/medium-sized-nevus-spilus-of-the-neck-treated-with-pulsed-dye-laser
#8
Vito Abrusci, Valentina Benzecry
Patients with large benign melanocytic lesions located on the neck represent a therapeutic challenge since the neck is a high-risk area for hypertrophic scarring and/or retraction. When treating a benign, extensive and visible melanocytic lesion, the choice of therapy mainly depends on the likelihood of improved cosmesis. We report a case of a 33-year-old woman with a medium-sized Nevus Spilus located on the neck, successfully treated with four sessions of pulsed dye laser. We achieved an excellent clinical and cosmetic result in such a challenging area...
April 20, 2017: Dermatologic Therapy
https://www.readbyqxmd.com/read/28424926/erratum-to-co2-erbium-yag-dye-laser-combination-an-effective-and-successful-treatment-for-angiofibromas-in-tuberous-sclerosis
#9
Paolo Fioramonti, Liliana De Santo, Martina Ruggieri, Sara Carella, Federico Lo Torto, Maria Giuseppina Onesti, Nicolò Scuderi
No abstract text is available yet for this article.
April 19, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28414151/targeted-antigen-delivery-to-dendritic-cell-via-functionalized-alginate-nanoparticles-for-cancer-immunotherapy
#10
Chuangnian Zhang, Gaona Shi, Ju Zhang, Huijun Song, Jinfeng Niu, Shengbin Shi, Pingsheng Huang, Yanming Wang, Weiwei Wang, Chen Li, Deling Kong
The purpose of the present study was to identify an "easy-to-adopt" strategy to enhance immune responses using functionalized alginate (ALG) nanoparticles (MAN-ALG/ALG=OVA NPs), which were prepared by CaCl2 cross-linking of two different types of ALG. The mannose (MAN) modified ALG (MAN-ALG) was used for dendritic cell targeting. The other component, composed of ovalbumin (OVA), a model antigen, is conjugated to ALG (ALG=OVA) via pH sensitive Schiff base bond. Grafting of alginate was demonstrated by FT-IR and (1)H NMR, while the morphological structure, particle size, Zeta potential of MAN-ALG/ALG=OVA NPs were measured using TEM and DLS...
April 13, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28413806/ryanodine-and-ip3-receptor-mediated-calcium-signaling-play-a-pivotal-role-in-neurological-infrared-laser-modulation
#11
Gleb P Tolstykh, Cory A Olsovsky, Bennett L Ibey, Hope T Beier
Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion ([Formula: see text]) sensitive dyes show changes in [Formula: see text] levels after various IR stimulation parameters, which suggests that [Formula: see text] may originate from the external solution...
April 2017: Neurophotonics
https://www.readbyqxmd.com/read/28412801/-preliminary-report-on-the-application-of-femtosecond-laser-assisted-anterior-capsulotomy-in-intumescent-white-cataract-surgery
#12
T T Peng, Y Wang, X Y Bao
Objective: To assess the effect of femtosecond laser-assisted anterior capsulotomy in intumescent white cataract surgery. Methods: Retrospective case series study. One hundred and fifty cataract patients (150 eyes) from June 2014 to June 2015 in Wuhan Aier ophthalmology hospital were enrolled in this study. They were divided into three groups: 50 intumescent white cataract patients (50 eyes) in the study group were performed femtosecond laser-assisted capsulotomy (LenSx system) operation selectively. Fifty intumescent white cataract patients (50 eyes) underwent normal manual continuous circular capsulorhexis as the control group 1...
April 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28411450/activatable-near-infrared-dye-conjugated-hyaluronic-acid-based-nanoparticles-as-a-targeted-theranostic-agent-for-enhanced-fluorescence-ct-photoacoustic-imaging-guided-photothermal-therapy
#13
Xiaolong Liang, Lei Fang, Xiaoda Li, Xu Zhang, Fan Wang
Targeted theranostic nano-system integrating functions of both diagnosis and therapy shows great potential for improving diagnosis and therapeutic efficacy. Herein, multifunctional nanoparticle based on activatable hyaluronic acid (HA) conjugating two near-infrared (NIR) dyes of Cy5.5 and IR825 was successfully designed and fabricated, and simultaneously used as a carrier for encapsulating perfluorooctylbromide (PFOB). In this system, PFOB showed good capability to absorb the X-rays, Cy5.5 on the outer surface acted as a fluorescent dye activatable by hyaluronidases (Hyals) in the tumor, and IR825 in the core as a photothermal agent...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28406508/integration-of-digital-microfluidics-with-whispering-gallery-mode-sensors-for-label-free-detection-of-biomolecules
#14
Sentayehu F Wondimu, Sebastian von der Ecken, Ralf Ahrens, Wolfgang Freude, Andreas E Guber, Christian Koos
We present a multi-sensor chip comprising an array of whispering-gallery mode (WGM) micro-goblet lasers integrated into a digital microfluidic (DMF) system. In contrast to earlier demonstrations, the lasers are fabricated from dye-doped poly-methyl methacrylate (PMMA) at low cost using spin-coating, mask-based optical lithography, wet chemical etching, and thermal reflow techniques. Pumping and read-out of the devices is accomplished via simple free-space optics, thereby allowing large-scale sensor arrays to be addressed...
April 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28406466/artificial-red-blood-cells-as-potential-photosensitizers-in-dye-laser-treatment-against-port-wine-stains
#15
REVIEW
Naoaki Rikihisa, Shoji Watanabe, Yoshiaki Saito, Hiromi Sakai
We suggest a novel method that uses artificial blood cells (hemoglobin vesicles, Hb-Vs) as photosensitizers in dye laser treatment (at 595-nm wavelength) for port-wine stains (i.e., capillary malformations presenting as red birthmarks) based on the results of animal experiments. As compared with human red blood cells, Hb-Vs have the same absorbance of 595 nm wavelength light and produce the same level of heat following dye laser irradiation. Small sized Hb-Vs (250 nm) distribute in the plasma phase in blood and tend to flow in the marginal zone of microvessels...
April 13, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28403266/laser-assisted-photodynamic-therapy-two-novel-protocols-for-enhanced-treatment-results
#16
Mary L Stevenson, Julie K Karen, Elizabeth K Hale
<p>Photodynamic therapy (PDT) uses a topical photosensitizing agent which is activated by a light source to cause destruction of specific cells. Commonly used for the treatment of actinic keratoses and photodamage, PDT can also be used for other conditions including acne and sebaceous hyperplasia. Here we report our experience with two treatment protocols. The first protocol utilizes laser assisted delivery of topical 5-aminolevulinic acid for enhanced efficacy of blue light photodynamic therapy in the treatment of actinic keratoses and photodamage...
April 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28401413/concentration-dependent-approach-for-accurate-determination-of-two-photon-absorption-cross-section-of-fluorescent-dye-molecule
#17
Sandeep Kumar Maurya, Chayan Dutta, Debabrata Goswami
We have investigated the concentration dependent of two-photon induced fluorescence (TPIF) in methanolic solution of Rhodamine 6G and Rhodamine B dye using 120 fs laser pulses at 780 nm, 76 MHz repetition rate. TPIF study of these dyes was compared with their respective one photon fluorescence intensity. We have shown the effect of chopper on TPIF intensity from Rhodamine dyes, which have shown direct influence on the determined TPA Cross section of these dyes.
April 11, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28397902/lasing-behavior-of-surface-functionalized-carbon-quantum-dot-rhb-composites
#18
Ashish Yadav, Liang Bai, Yanmei Yang, Juan Liu, Ajeet Kaushik, Gary J Cheng, Lin Jiang, Lifeng Chi, Zhenhui Kang
Random lasers have been extensively and intensively investigated due to their fundamental importance and promising applications. Here, we explored the lasing behavior of carbon quantum dot (CQD)/Rhodamine B (RhB) composites. CQDs exhibited a broad emission spectrum which overlapped with the absorption spectrum of the RhB dye. We investigated an approach wherein the -OH, -NH2 and -PO4 group functionalized CQD/RhB composites showed lasing behavior. The optical pumping of functionalized CQD/RhB composites exhibited lasing emission which is dynamically tunable as a function of the surface properties of CQDs at the laser wavelength of 532 nm by varying the pump energy...
April 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28388282/combined-pulsed-dye-laser-and-fiberoptic-nd-yag-laser-for-the-treatment-of-hypertrophic-port-wine-stain
#19
Mohammed Radmanesh, Ramin Radmanesh
The hypertrophic port wine stain (PWS) is only partially and superficially treated with the Pulsed dye laser(PDL) because of its limited depth of penetration. We used combined PDL and fiberoptic 1444nm Nd-YAG laser to treat a case with hypertrophic PWS. METHOD: After tumescent anesthesia, few holes were made by a 16 gauge needle on different sides of the lesion. The fiberoptic tip was of 1444nm Nd-YAG laser was inserted within the holes and was pushed forward while triggering. In a fan pattern and by a back and forth movement, the subcutaneous and deep dermal areas were coagulated...
April 7, 2017: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/28387354/highly-ph-responsive-sensor-based-on-amplified-spontaneous-emission-coupled-to-colorimetry
#20
Qi Zhang, Jose R Castro Smirnov, Ruidong Xia, Jose M Pedrosa, Isabel Rodriguez, Juan Cabanillas-Gonzalez, Wei Huang
We demonstrated a simple, directly-readable approach for high resolution pH sensing. The method was based on sharp changes in Amplified Spontaneous Emission (ASE) of a Stilbene 420 (ST) laser dye triggered by the pH-dependent absorption of Bromocresol Green (BG). The ASE threshold of BG:ST solution mixtures exhibited a strong dependence on BG absorption, which was drastically changed by the variations of the pH of BG solution. As a result, ASE on-off or off-on was observed with different pH levels achieved by ammonia doping...
April 7, 2017: Scientific Reports
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