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https://www.readbyqxmd.com/read/28528603/a-retrospective-study-of-multiple-sequential-light-and-laser-sources-to-activate-aminolevulinic-acid-in-the-treatment-of-acne-vulgaris
#1
Daniel P Friedmann, Mitchel P Goldman, Sabrina G Fabi, Isabella Guiha
Reports of the sequential use of multiple light and laser sources for topical 5-aminolevulinic acid (ALA) activation in photodynamic therapy (PDT) of inflammatory acne vulgaris are lacking. The authors sought to retrospectively compare field-directed ALA-PDT with blue light only, blue light + pulsed dye laser (PDL), blue light + intense pulsed light (IPL), blue light + PDL + IPL, or blue light + red light + PDL + IPL for inflammatory acne of the face or upper trunk. Results showed a trend toward greater patient-reported improvement with comparable tolerability using multiple, sequential light sources in ALA-PDT for acne vulgaris, albeit not statistically significant...
2017: Skinmed
https://www.readbyqxmd.com/read/28521677/molluscum-contagiosum-an-update
#2
Alexander Kc Leung, Benjamin Barankin, Kam Lun Hon
BACKGROUND: Molluscum contagiosum is a viral cutaneous infection in childhood that occurs worldwide. Physicians should familiarize themselves with this common condition. OBJECTIVE: To review in depth the epidemiology, pathophysiology, clinical manifestations, complications and, in particular, treatment of molluscum contagiosum. METHODS: A PubMed search was completed in Clinical Queries using the key term "molluscum contagiosum". Patents were searched using the key term "molluscum contagiosum" from www...
May 17, 2017: Recent Patents on Inflammation & Allergy Drug Discovery
https://www.readbyqxmd.com/read/28520896/laser-assisted-indocyanine-green-dye-angiography-for-postoperative-fistulas-after-salvage-laryngectomy
#3
Erin J Partington, Lindsay S Moore, Russel Kahmke, Jason M Warram, William Carroll, Eben L Rosenthal, Benjamin J Greene
Importance: Pharyngocutaneous fistula formation is an unfortunate complication after salvage laryngectomy for head and neck cancer that is difficult to anticipate and related to a variety of factors, including the viability of native pharyngeal mucosa. Objective: To examine whether noninvasive angiography with indocyanine green (ICG) dye can be used to evaluate native pharyngeal vascularity to anticipate pharyngocutaneous fistula development. Design, Setting, and Participants: This cohort study included 37 patients enrolled from June 1, 2013, to June 1, 2016, and follow-up was for at least 1 month postoperatively...
May 18, 2017: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28518118/harnessing-the-dna-dye-triggered-side-population-phenotype-to-detect-and-purify-cancer-stem-cells-from-biological-samples
#4
Maximilian Boesch, Elisabeth Hoflehner, Dominik Wolf, Guenther Gastl, Sieghart Sopper
Cancer is a stem cell-driven disease and eradication of these cells has become a major therapeutic goal. Deciphering vulnerabilities of Cancer Stem Cells (CSCs) and identifying suitable molecular targets relies on methods that allow their specific discrimination in heterogeneous samples such as cell lines and ex vivo tumor tissue. Flow cytometry/FACS is a powerful technology to multi-parametrically dissect biological samples at the single cell level and is to date the method of choice to recover live cells for downstream analyses...
May 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518092/isolation-of-the-side-population-in-myc-induced-t-cell-acute-lymphoblastic-leukemia-in-zebrafish
#5
Margaret M Pruitt, Wilfredo Marin, Michael R Waarts, Jill L O de Jong
Heterogeneous cell populations, from either healthy or malignant tissues, may contain a population of cells characterized by a differential ability to efflux the DNA-binding dye Hoechst 33342. This "side population" of cells can be identified using flow cytometric methods after the Hoechst 33342 dye is excited by an ultraviolet (UV) laser. The side population of many cell types contains stem- or progenitor-like cells. However, not all cell types have an identifiable side population. Danio rerio, zebrafish, have a robust in vivo model of T-cell acute lymphoblastic leukemia (T-ALL), but whether these zebrafish T-ALLs have a side population is unknown...
May 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28517926/laser-induced-population-inversion-in-rhodamine-6g-for-lysozyme-oligomers-detection
#6
Piotr Hanczyc, Lech Sznitko
Fluorescence spectroscopy is a common method for detecting amyloid fibrils where organic fluorophores are used as markers that exhibit increase of the quantum yield upon binding. However, most of the dyes exhibit enhanced emission only when bound to mature fibrils and significantly weaker signals are obtained in presence of amyloid oligomers. In the concept of population inversion, laser is used as an excitation source in order to keep the major fraction of molecules in the excited state to create the pathways for stimulated emission occurrence...
May 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28513655/boron-difluoride-hemicurcuminoid-as-an-efficient-far-red-to-near-infrared-emitter-toward-oleds-and-laser-dyes
#7
A D'Aléo, M H Sazzad, D H Kim, E Y Choi, J W Wu, G Canard, F Fages, J-C Ribierre, C Adachi
A hemicurcuminoid boron difluoride complex is used as an emitter in organic light-emitting diodes, showing far red/near-infrared electroluminescence with an external quantum efficiency as high as 2.1%. This dye blended in CBP thin films shows amplified spontaneous emission with a threshold of 22 μJ cm(-2) at 750 nm, making this compound attractive for organic semiconductor lasers operating in the near-infrared region.
May 17, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28512573/photoreduction-and-validation-of-haem-ligand-intermediate-states-in-protein-crystals-by-in-situ-single-crystal-spectroscopy-and-diffraction
#8
Demet Kekilli, Tadeo Moreno-Chicano, Amanda K Chaplin, Sam Horrell, Florian S N Dworkowski, Jonathan A R Worrall, Richard W Strange, Michael A Hough
Powerful synergies are available from the combination of multiple methods to study proteins in the crystalline form. Spectroscopies which probe the same region of the crystal from which X-ray crystal structures are determined can give insights into redox, ligand and spin states to complement the information gained from the electron-density maps. The correct assignment of crystal structures to the correct protein redox and ligand states is essential to avoid the misinterpretation of structural data. This is a particular concern for haem proteins, which can occupy a wide range of redox states and are exquisitely sensitive to becoming reduced by solvated electrons generated from interactions of X-rays with water molecules in the crystal...
May 1, 2017: IUCrJ
https://www.readbyqxmd.com/read/28511874/photothermal-and-photodynamic-activity-of-polymeric-nanoparticles-based-on-%C3%AE-tocopheryl-succinate-raft-block-copolymers-conjugated-to-ir-780
#9
Raquel Palao-Suay, Francisco M Martin-Saavedra, María Rosa Aguilar, Clara Escudero Duch, Sergio Martín-Saldaña, Francisco J Parra-Ruiz, Nathan A Rohner, Susan N Thomas, Nuria Vilaboa, Julio San Román
The aim of this work was the generation of a multifunctional nanopolymeric system that incorporates IR-780 dye, a near-infrared (NIR) imaging probe that exhibits photothermal and photodynamic properties; and a derivate of α-tocopheryl succinate (α-TOS), a mitochondria-targeted anticancer compound. IR-780 was conjugated to the hydrophilic segment of copolymer PEG-b-polyMTOS, based on poly(ethylene glycol) (PEG) and a methacrylic derivative of α-tocopheryl succinate (MTOS), to generate IR-NP, self-assembled nanoparticles (NPs) in aqueous media which exhibit a hydrophilic shell and a hydrophobic core...
May 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28508468/visible-light-responsive-color-switching-driven-by-photocatalytic-self-doped-sno2-x-nanocrystals
#10
Dan Han, Baolai Jiang, Ji Feng, Yadong Yin, Wenshou Wang
Visible light-responsive reversible color switching systems are attractive to many applications since visible light has superior penetration and causes far less damages to organic molecules than UV. Here we report that self-doping of Sn2+ in SnO2-x nanocrystals red shifts their absorption to visible region and simultaneously produces oxygen vacancies, which can effectively scavenge photogenerated holes and thus enable color switching of redox dyes using visible light. Wavelength selective switching can also be achieved by coupling the photocatalytic activity of the SnO2-x NCs with the color switching kinetics of different redox dyes...
May 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28507898/optically-and-acoustically-triggerable-sub-micron-phase-change-contrast-agents-for-enhanced-photoacoustic-and-ultrasound-imaging
#11
Shengtao Lin, Anant Shah, Javier Hernández-Gil, Antonio Stanziola, Bethany I Harriss, Terry O Matsunaga, Nicholas Long, Jeffrey Bamber, Meng-Xing Tang
We demonstrate a versatile phase-change sub-micron contrast agent providing three modes of contrast enhancement: 1) photoacoustic imaging contrast, 2) ultrasound contrast with optical activation, and 3) ultrasound contrast with acoustic activation. This agent, which we name 'Cy-droplet', has the following novel features. It comprises a highly volatile perfluorocarbon for easy versatile activation, and a near-infrared optically absorbing dye chosen to absorb light at a wavelength with good tissue penetration...
June 2017: Photoacoustics
https://www.readbyqxmd.com/read/28507428/propranolol-therapy-for-infantile-hemangioma-our-experience
#12
Ling Zhang, Hai-Wei Wu, Weien Yuan, Jia-Wei Zheng
OBJECTIVE: Hemangiomas are the most common benign vascular tumors of infancy. Although most infantile hemangiomas (IHs) have the ability to involute spontaneously after initial proliferation and resolve without consequence, intervention is required in a subset of IHs, which develop complications resulting in ulceration, bleeding, or aesthetic deformity. The primary treatment for this subset of IHs is pharmacological intervention, and propranolol has become the new first-line treatment for complicated hemangiomas...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28503995/preparation-and-characterization-of-surface-modified-plga-polymeric-nanoparticles-used-to-target-treatment-of-intestinal-cancer
#13
Niyaz Ahmad, Md Aftab Alam, Rizwan Ahmad, Atta Abbas Naqvi, Farhan Jalees Ahmad
Docetaxel (DTX), a cytotoxic taxane, is a poor water-soluble drug and exhibits less oral bioavailability. Current research investigates the effective transport, for DTX-loaded chitosan (CS)-coated-poly-lactide-co-glycolide (PLGA)-nanoparticles (NPs) (DTX-CS-PLGA-NPs) and DTX-PLGA-NPs as well as a novel third-generation P-gp inhibitor i.e. GF120918 (Elacridar), across intestinal epithelium with its successive uptake by the tumour cells in an in vitro model. The prepared NPs showed a spherical shape particle size i...
May 14, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28501188/a-hemicyanine-based-colorimetric-and-ratiometric-fluorescent-probe-for-selective-detection-of-cysteine-and-bioimaging-in-living-cell
#14
Guotao Liu, Dan Liu, Xie Han, Xiaole Sheng, Zhiqiang Xu, Sheng Hua Liu, Lintao Zeng, Jun Yin
A new ratiometric fluorescent probe based on a hemicyanine dye was synthesized. Investigation on the response behavior towards amino acids showed that the probe had a capability of rapidly and selectively detecting cysteine over other biothiols such as glutathione and homocysteine with similar structure and reactivity, and it also displayed a high selectivity and rapid response. Moreover, the probe had good water solubility, which authorized it could be applied in fluorescent bioimaging. The laser confocal fluorescence images indicated that the probe could visualize the intracellular cysteine...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28494960/laser-activated-polymeric-microcapsules-for-ultrasound-imaging-and-therapy-in%C3%A2-vitro-feasibility
#15
Guillaume Lajoinie, Tom van Rooij, Ilya Skachkov, Emilie Blazejewski, Gert Veldhuis, Nico de Jong, Klazina Kooiman, Michel Versluis
Polymeric microcapsules with a light-absorbing dye incorporated in their shell can generate vapor microbubbles that can be spatiotemporally controlled by pulsed laser irradiation. These contrast agents of 6-8 μm in diameter can circulate through the vasculature, offering possibilities for ultrasound (molecular) imaging and targeted therapies. Here, we study the impact of such vapor bubbles on human endothelial cells in terms of cell poration and cell viability to establish the imaging and therapeutic windows...
May 9, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28494055/modern-management-of-nasal-hemangiomas
#16
Robert G Keller, Shawn Stevens, Marcelo Hochman
Importance: Current treatment approaches for infantile hemangiomas of the nose include observation, pharmacologic agents, surgery, and/or laser therapy. Because of the known functional, social, and cosmetic effect of nasal deformities, obtaining the best possible result is critical. Optimal timing, type, duration, and extent of therapy remain unclear. Observations: Results of a review of 86 patients (64 females and 22 males; mean age, 4.8 months [range, 2 days-23 years]) with infantile hemangiomas of the nose treated from January 1, 1999, to December 31, 2015, and a review of the literature are presented to gain insight into the preferred approach to the treatment of these lesions...
May 11, 2017: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/28493330/improved-panels-for-clinical-immune-phenotyping-utilization-of-the-violet-laser
#17
Mark Ryherd, Matthew Plassmeyer, Connor Alexander, Ines Eugenio, Yuliya Kleschenko, Ariel Badger, Raavi Gupta, Oral Alpan, Søren Ulrik Sønder
BACKGROUND: Clinical diagnostic laboratories are subject to numerous regulations imposed by government agencies. Laboratory developed tests for flow cytometry panels are essentially restricted to the use of analyte-specific reagents (ASR) antibodies. With the advances in clinical flow cytometry systems, there is a trend towards the utilization of blue/red/violet laser flow systems and 8 to 10-color panels. Currently, the selection of commercially available ASR antibodies for the violet laser is very limited...
May 10, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28492660/selective-vesicle-aggregation-achieved-via-the-self-assembly-of-terpyridine-based-building-blocks
#18
Guiping Wang, Yuanyuan Peng, Lingyun Lou, Pengyao Xing, Guangyan Du
Herein, we report the self-assembly of a mono terpyridine-based building block modified with long alkyl chains, which gives rise to vesicular aggregates in aqueous media. The vesicles are responsive to transition metal ions, and form different kinds of aggregates after metal-ligand coordination. In particular, Ni(ii) shows a unique influence on morphological transitions, whereby vesicles aggregate and fuse upon the addition of Ni(ii) ions. Spectroscopic and morphological studies are highlighted in this work...
May 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/28492633/rational-molecular-design-enhancing-the-photonic-performance-of-red-emitting-perylene-bisimide-dyes
#19
E Avellanal-Zaballa, G Durán-Sampedro, A Prieto-Castañeda, A R Agarrabeitia, I García-Moreno, I López-Arbeloa, J Bañuelos, M J Ortiz
We report the synthesis of novel multichromophoric organic architectures, where perylene red is decorated with BODIPY and/or hydroxycoumarin dyes acting as light harvesters and energy donors. The computationally-aided photophysical study of these molecular assemblies reveals a broadband absorption which, regardless of the excitation wavelength, leads solely to a bright red-edge emission from perylene bisimide after efficient intramolecular energy transfer hops. The increase of the absorbance of these molecular antennas at key pumping wavelengths enhances the laser action of the commercial perylene red...
May 11, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28488393/in-vitro-analysis-of-early-calcification-in-aortic-valvular-interstitial-cells-using-laser-induced-breakdown-spectroscopy-libs
#20
Seyyed Ali Davari, Shirin Masjedi, Zannatul Ferdous, Dibyendu Mukherjee
Calcific aortic valve disease (CAVD) is a major cardiovascular disorder caused by osteogenic differentiation of valvular interstitial cells (VICs) within aortic valves. Conventional methods like colorimetric assays and histology fail to detect small calcium depositions during in-vitro VIC cultures. Laser-induced breakdown spectroscopy (LIBS) is a robust analytical tool used for inorganic materials characterizations, but relatively new to biomedical applications. We employ LIBS, for the first time, for quantitative in-vitro detection of calcium depositions in VICs at various osteogenic differentiation stages...
May 9, 2017: Journal of Biophotonics
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