keyword
MENU ▼
Read by QxMD icon Read
search

Plasmin

keyword
https://www.readbyqxmd.com/read/29777136/functional-proteomic-profiling-of-secreted-serine-proteases-in-health-and-inflammatory-bowel-disease
#1
Alexandre Denadai-Souza, Chrystelle Bonnart, Núria Solà Tapias, Marlène Marcellin, Brendan Gilmore, Laurent Alric, Delphine Bonnet, Odile Burlet-Schiltz, Morley D Hollenberg, Nathalie Vergnolle, Céline Deraison
While proteases are essential in gastrointestinal physiology, accumulating evidence indicates that dysregulated proteolysis plays a pivotal role in the pathophysiology of inflammatory bowel disease (IBD). Nonetheless, the identity of overactive proteases released by human colonic mucosa remains largely unknown. Studies of protease abundance have primarily investigated expression profiles, not taking into account their enzymatic activity. Herein we have used serine protease-targeted activity-based probes (ABPs) coupled with mass spectral analysis to identify active forms of proteases secreted by the colonic mucosa of healthy controls and IBD patients...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29767556/hydronephrosis-is-associated-with-elevated-plasmin-in-urine-in-pediatric-patients-and-rats-and-changes-in-ncc-and-%C3%AE-enac-abundance-in-rat-kidney
#2
Rikke Zachar, Ammar Al-Mashhadi, Henrik Dimke, Per Svenningsen, Boye L Jensen, Mattias Carlström
Obstruction of urine flow at the level of the pelvo-ureteric junction (UPJO) and subsequent development of hydronephrosis is one of the most common congenital renal malformations. UPJO is associated with development of salt-sensitive hypertension, which is set by the obstructed kidney, and with a stimulated renin-angiotensin-aldosterone system (RAAS) in rodent models. This study aimed at investigating the hypothesis that i) in pediatric patients with UPJO the RAAS is activated prior to surgical relief of the obstruction; ii) in rats with UPJO the RAAS activation is reflected by increased abundance of renal aldosterone-stimulated Na+ transporters; and iii) the injured UPJO kidney allows aberrant filtration of plasminogen leading to proteolytic activation of the epithelial sodium channel gamma subunit (γ-ENaC)...
May 16, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29752284/analysis-of-the-thrombotic-and-fibrinolytic-activities-of-tumor-cell-derived-extracellular-vesicles
#3
Ludovic Durrieu, Alamelu Bharadwaj, David M Waisman
Exosomes and microvesicles (MVs) are small extracellular vesicles secreted by tumor cells and are suggested to contribute to the thrombotic events that commonly occur in patients with advanced malignancies. Paradoxically, these vesicles have been reported to also possess fibrinolytic activity. To determine whether thrombotic or fibrinolytic activity is a predominant characteristic of these extracellular vesicles, we prepared exosomes and MVs from 2 breast cancer cell lines (MDA-MB-231 and MCF7), a lung cancer cell line (A549), and a leukemia cell line (NB4) and assayed their thrombotic and fibrinolytic activities...
May 22, 2018: Blood Advances
https://www.readbyqxmd.com/read/29746547/combining-different-proteomic-approaches-to-resolve-complexity-of-the-milk-protein-fraction-of-dromedary-bactrian-camels-and-hybrids-from-different-regions-of-kazakhstan
#4
Alma Ryskaliyeva, Céline Henry, Guy Miranda, Bernard Faye, Gaukhar Konuspayeva, Patrice Martin
Nutritional suitability of milk is not only related to gross composition, but is also strongly affected by the microheterogeniety of the protein fraction. Hence, to go further into the evaluation of the potential suitability of non-bovine milks in human/infant nutrition it is necessary to have a detailed characterization of their protein components. Combining proven proteomic approaches (SDS-PAGE, LC-MS/MS and LC-ESI-MS) and cDNA sequencing, we provide here in depth characterization of the milk protein fraction of dromedary and Bactrian camels, and their hybrids, from different regions of Kazakhstan...
2018: PloS One
https://www.readbyqxmd.com/read/29739066/basic-evaluation-of-the-newly-developed-lias-auto-p-fdp-assay-and-the-influence-of-plasmin-%C3%AE-2-plasmin-inhibitor-complex-values-on-discrepancy-in-the-comparison-with-lias-auto-d-dimer-neo-assay
#5
Osamu Kumano, Masahiro Ieko, Yutaka Komiyama, Sumiyoshi Naito, Mika Yoshida, Nobuhiko Takahashi, Kazumasa Ohmura, Junki Hayasaki, Mineji Hayakawa
BACKGROUND: Laboratory determination of fibrin/fibrinogen degradation products (FDP) levels, along with that of the D-dimer, is important for assessing the fibrinolytic situation. Recently, we developed a new FDP reagent "Lias Auto P-FDP", which can detect various FDP fragments. The purpose of this study was to evaluate the basic performance of the newly developed Lias Auto P-FDP and compare it with Lias Auto D-Dimer Neo assay. METHODS: The within-run precision of Lias Auto P-FDP and Lias Auto D-Dimer was determined 20 times in low and high value controls...
April 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29729197/excessive-plasmin-compromises-hepatic-sinusoidal-vascular-integrity-after-acetaminophen-overdose
#6
Siqi Gao, Robert Silasi-Mansat, Mandi Behar, Florea Lupu, Courtney T Griffin
The serine protease plasmin degrades extracellular matrix (ECM) components both directly and indirectly through activation of matrix metalloproteinases. Excessive plasmin activity and subsequent ECM degradation causes hepatic sinusoidal fragility and hemorrhage in developing embryos. We report here that excessive plasmin activity in a murine acetaminophen (APAP) overdose model likewise compromises hepatic sinusoidal vascular integrity in adult animals. We found that hepatic plasmin activity is upregulated significantly at 6 hr after APAP overdose...
May 5, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29726798/computational-simulations-assessment-of-mutations-impact-on-streptokinase-sk-from-a-group-g-streptococci-with-enhanced-activity-insights-into-the-functional-roles-of-structural-dynamics-flexibility-of-sk-and-stabilization-of-sk-%C3%AE-plasmin-catalytic-complex
#7
Faegheh Kazemi, Seyed Shahriar Arab, Nasir Mohajel, Malihe Keramati, Niloofar Niknam, Mohammad Mehdi Aslani, Farzin Roohvand
Streptokinase (SK), a plasminogen activator (PA) that converts inactive plasminogen (Pg) to plasmin (Pm), is a protein secreted by groups A, C and G streptococci (GAS, GCS and GGS, respectively), with high sequence divergence and functional heterogeneity. While roles of some residual changes in altered SK functionality are shown, the underlying structural mechanisms are less known. Herein, using computational approaches, we analyzed the conformational basis for the increased activity of SK from a GGS (SKG132) isolate with four natural residual substitutions (Ile33Phe, Arg45Gln, Asn228Lys, Phe287Ile) compared to the standard GCS (SKC)...
May 4, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29724819/establishing-the-transient-mass-balance-of-thrombosis-from-tissue-factor-to-thrombin-to-fibrin-under-venous-flow
#8
Shu Zhu, Jason Chen, Scott L Diamond
OBJECTIVE: We investigated the coregulation of thrombin and fibrin as blood flows over a procoagulant surface. APPROACH AND RESULTS: Using microfluidic perfusion of factor XIIa-inhibited human whole blood (200 s- 1 wall shear rate) over a 250-μ long patch of collagen/TF (tissue factor; ≈1 molecule per μm2 ) and immunoassays of the effluent for F1.2 (prothrombin fragment 1.2), TAT (thrombin-antithrombin complex), and D-dimer (post-end point plasmin digest), we sought to establish the transient mass balance for clotting under venous flow...
May 3, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29724147/characteristics-of-fibrinolytic-disorders-in-acute-promyelocytic-leukemia
#9
Ping Wang, Yingmei Zhang, Huiyuan Yang, Wenyi Hou, Bo Jin, Jinxiao Hou, Haitao Li, Hongli Zhao, Jin Zhou
OBJECTIVES: Catastrophic hemorrhage remains the main cause of acute promyelocytic leukemia (APL) treatment failure. This study was aimed to study the pathogenesis of coagulopathy in patients with APL. METHODS: Multiple procoagulant and profibrinolytic parameters in plasma and peripheral leukocytes from 24 patients with newly diagnosed APL accompanied by coagulopathy before and after arsenic trioxide (ATO) treatment were evaluated. RESULTS: Prior to the treatment, the patients had elevated D-dimer and decreased fibrinogen levels...
May 4, 2018: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/29723259/generation-and-characterization-of-monoclonal-antibodies-against-the-n-terminus-of-alpha-2-antiplasmin
#10
Shiraazkhan Abdul, Miet Peeters, Els Brouwers, Joyce J M C Malfliet, Frank W G Leebeek, Paul J Declerck, Dingeman C Rijken, Shirley Uitte de Willige
Around 70% of circulating alpha-2-antiplasmin (α2AP), the main natural plasmin inhibitor, is N-terminally cleaved between residues Pro12 and Asn13 by antiplasmin-cleaving enzyme. This converts native Met-α2AP into the more potent fibrinolysis inhibitor Asn-α2AP. The Arg6Trp (R6W) polymorphism affects the N-terminal cleavage rate of Met-α2AP in a purified system, with ~8-fold faster conversion of Met(R6)-α2AP than Met(W6)-α2AP. To date, assays to determine N-terminally intact Met-α2AP in plasma have been limited to an ELISA that only measures Met(R6)-α2AP...
2018: PloS One
https://www.readbyqxmd.com/read/29723163/a-fibrin-biofilm-covers-the-blood-clot-and-protects-from-microbial-invasion
#11
Fraser L Macrae, Cédric Duval, Praveen Papareddy, Stephen R Baker, Nadira Yuldasheva, Katherine J Kearney, Helen R McPherson, Nathan Asquith, Joke Konings, Alessandro Casini, Jay L Degen, Simon D Connell, Helen Philippou, Alisa S Wolberg, Heiko Herwald, Robert As Ariëns
Haemostasis requires conversion of fibrinogen to fibrin fibres that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining the clot. We demonstrated that only fibrin is required to form the film, and that it occurred in vitro and in vivo...
May 3, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29720403/urokinase-type-plasminogen-activator-upa-promotes-ezrin-mediated-reorganization-of-the-synaptic-cytoskeleton-in-the-ischemic-brain
#12
Paola Merino, Ariel Diaz, Luis Guillermo Manrique, Lihong Cheng, Manuel Yepes
Synaptic repair in the ischemic brain is a complex process that requires reorganization of the actin cytoskeleton.  Ezrin, radixin and moesin (ERM) are a group of evolutionary conserved proteins that link the plasma membrane to the actin cytoskeleton and act as scaffolds for signaling transduction.  Urokinase-type plasminogen activator (uPA) is a serine proteinase that upon binding to the urokinase-type plasminogen activator receptor (uPAR) catalyzes the conversion of plasminogen into plasmin on the cell surface and activates intracellular signaling pathways...
May 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29701177/a-case-of-infection-related-glomerulonephritis-with-massive-eosinophilic-infiltration
#13
Masahiro Okabe, Tsuyoshi Takamura, Akiko Tajiri, Nobuo Tsuboi, Masahiro Ishikawa, Makoto Ogura, Ryuji Ohashi, Takashi Oda, Takashi Yokoo
Infection-related glomerulonephritis (IRGN) is rarely complicated with eosinophil infiltration into the glomerulus. Here we report a case of eosinophilic proliferative glomerulonephritis related with infection. A 70-year-old man with respiratory symptoms displayed hypereosinophilia, hypocomplementemia, impaired renal function, and nephrotic syndrome. Renal biopsy revealed endocapillary proliferative glomerulonephritis with immunostaining for immunoglobulin G and complement 3, and subepithelial hump-like electron-dense deposits, thus fulfilling the criteria for IRGN...
April 27, 2018: Clinical Nephrology
https://www.readbyqxmd.com/read/29691836/early-life-regional-and-temporal-variation-in-filaggrin-derived-natural-moisturising-factor-filaggrin-processing-enzyme-activity-corneocyte-phenotypes-and-plasmin-activity-implications-for-atopic-dermatitis
#14
M A McAleer, I Jakasa, N Raj, C P F O'Donnell, M E Lane, A V Rawlings, R Voegeli, W H I McLean, S Kezic, A D Irvine
BACKGROUND: Filaggrin is central to the pathogenesis of atopic dermatitis (AD). The cheeks are a common initiation site of infantile AD. Regional and temporal expression of levels of filaggrin degradation products (natural moisturising factors, NMF), activities of filaggrin processing enzymes (bleomycin hydrolase; BH and calpain-1; C-1) and plasmin, and corneocyte envelope (CE) maturity in early life are largely unknown. OBJECTIVES: We did a cross-sectional, observational study investigating regional and age dependent variations in NMF levels, activity of proteases and CE maturity in stratum corneum (SC) from infants to determine if these could explain the observed predilection sites for AD in early life...
April 25, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29681849/danhong-injection-combined-with-t-pa-improves-thrombolytic-therapy-in-focal-embolic-stroke
#15
Min Li, Jing Zhou, Weifeng Jin, Xiaohong Li, Yuyan Zhang
Background: Hemorrhagic transformation, neurotoxicity, short treatment time windows, and other defects are considered as the major limitations for the thrombolytic therapy. This study is devoted to figure out whether Danhong injection (DHI) combined with tissue-plasminogen activator (t-PA) could extend the treatment time windows and ameliorate brain injury, hemorrhagic complication and BBB disruption after focal embolic stroke. Methods: In vitro , the combined concentrations of DHI and t-PA were added to wells reacted with plasminogen and D-Val-Leu-Lys-AMC...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29679322/three-dimensional-hydrogel-based-culture-to-study-the-effects-of-toxicants-on-ovarian-follicles
#16
Hong Zhou, Ariella Shikanov
Various toxicants, such as drugs and their metabolites, can cause potential ovarian toxicity. As the functional units of the ovary, ovarian follicles are susceptible to this type of damage at all developmental stages. Studying the effects of toxicants on ovarian follicles is an important task. Three-dimensional (3D) hydrogels, such as fibrin alginate interpenetrating networks (FA-IPNs), can support ovarian follicle culture in vitro for extended periods of time and serve as a suitable tool for studying ovotoxicity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29669808/lactoferrin-is-a-natural-inhibitor-of-plasminogen-activation
#17
Alexander Zwirzitz, Michael Reiter, Rostislav Skrabana, Anna Ohradanova-Repic, Otto Majdic, Marianna Gutekova, Ondrej Cehlar, Eva Petrovčíková, Eva Kutejova, Gerold Stanek, Hannes Stockinger, Vladimir Leksa
The plasminogen system is essential for dissolution of fibrin clots, and in addition, it is involved in a wide variety of other physiological processes, including proteolytic activation of growth factors, cell migration, and removal of protein aggregates. On the other hand, uncontrolled plasminogen activation contributes to many pathological processes, e.g. tumor cells' invasion in cancer progression. Moreover, some virulent bacterial species, e.g. Streptococci or Borrelia, bind human plasminogen and hijack the host's plasminogen system to penetrate tissue barriers...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29664836/teg-lysis-shutdown-represents-coagulopathy-in-bleeding-trauma-patients-analysis-of-the-proppr-cohort
#18
Jessica C Cardenas, Charles E Wade, Bryan A Cotton, Mitchell J George, John B Holcomb, Martin A Schreiber, Nathan J White
BACKGROUND: Thrombelastography (TEG) fibrinolysis shutdown after trauma is associated with increased mortality due to hypercoagulability-associated organ failure. However, a lack of mechanistic data has precluded the development of novel interventions to treat shutdown. OBJECTIVES: To define the pathophysiology of TEG shutdown in severely injured, bleeding patients through secondary analysis of the PROPPR trial. METHODS: Fibrinolysis was characterized in PROPPR subjects using admission TEG lysis at 30 minutes (LY30) or plasmin-antiplasmin (PAP) levels...
April 16, 2018: Shock
https://www.readbyqxmd.com/read/29657009/molecular-and-biochemical-characterization-of-taenia-solium-%C3%AE-enolase
#19
Shaohua Zhang, Yanan You, Xuenong Luo, Yadong Zheng, Xuepeng Cai
Enolase (EC 4.2.1.11) acts as a multifunctional enzyme in many organisms, being involved in metabolism, transcription regulation and pathogenesis. In the current study, the recombinant α-enolase from Taenia solium (His-Tseno) was prepared and antiserum against His-Tseno was generated in rabbits. Consequently, we analyzed the enzymatic characteristics, plasminogen binding activity, tissue localization and expression patterns of Tseno. The study demonstrated that the enzymatic activity of His-Tseno was enhanced at pH around 7...
April 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29629340/enterokinase-enhances-influenza-a-virus-infection-by-activating-trypsinogen-in-human-cell-lines
#20
Hideki Hayashi, Yoshinao Kubo, Mai Izumida, Etsuhisa Takahashi, Hiroshi Kido, Ko Sato, Mutsuo Yamaya, Hidekazu Nishimura, Kou Nakayama, Toshifumi Matsuyama
Cleavage and activation of hemagglutinin (HA) by trypsin-like proteases in influenza A virus (IAV) are essential prerequisites for its successful infection and spread. In host cells, some transmembrane serine proteases such as TMPRSS2, TMPRSS4 and HAT, along with plasmin in the bloodstream, have been reported to cleave the HA precursor (HA0 ) molecule into its active forms, HA1 and HA2 . Some trypsinogens can also enhance IAV proliferation in some cell types (e.g., rat cardiomyoblasts). However, the precise activation mechanism for this process is unclear, because the expression level of the physiological activator of the trypsinogens, the TMPRSS15 enterokinase, is expected to be very low in such cells, with the exception of duodenal cells...
2018: Frontiers in Cellular and Infection Microbiology
keyword
keyword
80502
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"