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https://www.readbyqxmd.com/read/28210718/synthesis-and-epr-spectroscopic-characterization-of-the-perchlorotriarylmethyl-tricarboxylic-acid-radical-ptmtc-and-its-13-c-labelled-analogue-13c-ptmtc
#1
Marwa Elewa, Nadica Maltar-Strmečki, Mohamed M Said, Hosam A El Shihawy, Mohamed El-Sadek, Juliane Frank, Simon Drescher, Malte Drescher, Karsten Mäder, Dariush Hinderberger, Peter Imming
A hydrophilic tris(tetrachlorotriaryl)methyl (tetrachloro-TAM) radical labelled 50% with (13)C at the central carbon atom was prepared. The mixture of isotopologue radicals was characterised by continuous wave and pulsed X-band electron paramagnetic spectroscopy (EPS). For the pharmaceutical and medical applications planned, the quantitative influence of oxygen, viscosity, temperature and pH on EPR line widths was studied in aqueous buffer, DMSO, water-methanol and water-glycerol mixtures. Under in vivo conditions, pH can be disregarded...
February 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28206759/first-evidence-of-vibrationally-driven-bimolecular-reactions-in-solution-the-reactions-of-br-atoms-with-dimethylsulfoxide-and-methanol
#2
Jae Yoon Shin, Michael A Shaloski, Forrest Fleming Crim, Amanda S Case
We present evidence for vibrational enhancement of the rate of the bimolecular reactions of Br atoms with dimethylsulfoxide (DMSO) and methanol (CH3OH) in the condensed phase. The abstraction of a hydrogen atom from either of these solvents by a Br atom is highly endoergic: 3269 cm(-1) for DMSO and 1416 or 4414 cm(-1) for CH3OH, depending on the hydrogen atom abstracted. Thus, there is no thermal abstraction reaction at room temperature. Broadband electronic transient absorption shows that following photolysis of bromine precursors, Br atoms form van der Waals complexes with the solvent molecules in about 5 ps and this Br(●)-solvent complex undergoes recombination...
February 16, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28202328/discovery-of-hyaluronidase-inhibitors-from-natural-products-and-their-mechanistic-characterization-under-dmso-perturbed-assay-conditions
#3
Keisuke Tomohara, Tomohiro Ito, Saika Onikata, Atsushi Kato, Isao Adachi
The present study discovered four novel hyaluronan-degrading enzyme (hyaluronidase) inhibitors including chikusetsusaponins and catechins through the activity-guided separation of Panax japonicus and Prunus salicina, respectively. Although the discovery resulted in identification of usual frequent hitters, subsequent mechanistic characterizations under our DMSO-perturbed assay conditions and related protocols revealed that chikusetusaponin IV would serve as an aggregating and non-specific binding inhibitor, while (-)-epicatechin would interact specifically with enzyme at the catalytic site or more likely at a kind of catechin-binding site with a relatively week inhibitory activity...
January 31, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28198521/sevoflurane-ameliorates-doxorubicin-induced-myocardial-injury-by-affecting-the-phosphorylation-states-of-proteins-in-pi3k-akt-mtor-signaling-pathway
#4
Yini Wu, Jianping Wang, Xiaoyan Yu, Dongli Li, Xin Han, Lihua Fan
BACKGROUND: The effect of sevoflurane on the doxorubicin-induced myocardial injury was explored by investigating the phosphorylation states of proteins in phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway. METHODS: Myocardial injury rat models were induced by doxorubicin and evenly assigned into five groups according to different treatment: Doxorubicin group (DG, 200-µL saline solution), sevoflurane group (SevG, inhaled with 2...
February 15, 2017: Cardiology Journal
https://www.readbyqxmd.com/read/28198137/membrane-fluidity-is-involved-in-the-regulation-of-heat-stress-induced-secondary-metabolism-in-ganoderma-lucidum
#5
Yong-Nan Liu, Tian-Jun Zhang, Xiao-Xiao Lu, Bao-Liang Ma, Ang Ren, Liang Shi, Ai-Liang Jiang, Han-Shou Yu, Ming-Wen Zhao
Ganoderma lucidum has become a potential model system for evaluating how environmental factors regulate the secondary metabolism of basidiomycetes. Heat stress (HS) is one of the most important environmental factors. It was previously reported that HS could induce the biosynthesis of ganoderic acids (GA). In this study, we found that HS increased GA biosynthesis and also significantly increased cell membrane fluidity. Furthermore, our results showed that addition of the membrane rigidifier DMSO could revert the increased GA biosynthesis elicited by HS...
February 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28198061/antiinflammatory-oxicams-as-multi-donor-ligand-systems-ph-and-solvent-dependent-coordination-modes-of-meloxicam-and-piroxicam-to-ru-and-os
#6
Farhana Aman, Muhammad Hanif, Mario Kubanik, Adnan Ashraf, Tilo Soehnel, Stephen Jamieson, Waseeq Siddiqui, Christian Hartinger
The nitrogen- and sulfur-containing 1,2-benzothiazines meloxicam and piroxicam are widely used as nonsteroidal antiinflammatory drugs. Intrigued by the presence of multiple donor atoms and therefore a potentially rich coordination chemistry, we prepared a series of organometallic Ru and Os compounds with meloxicam and piroxicam featuring either as mono- or bidentate ligand systems. The choice of the solvent and the pH value was identified as the critical parameter to achieve selectively mono- or bidentate coordination...
February 14, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28197586/determination-of-the-temperature-dependent-cell-membrane-permeabilities-using-microfluidics-with-integrated-flow-and-temperature-control
#7
Cifeng Fang, Fujun Ji, Zhiquan Shu, Dayong Gao
We developed an integrated microfluidic platform for instantaneous flow and localized temperature control. The platform consisted of a flow-focusing region for sample delivery and a cross-junction region embedded with a microheater for cell trapping and localized temperature control by using an active feedback control system. We further used it to measure the membrane transport properties of Jurkat cells, including the osmotically inactive cell volume (Vb) and cell membrane permeabilities to water (Lp) and to cryoprotective agent (CPA) solutions (dimethyl sulfoxide (DMSO) in this study) (PS) at various temperatures (room temperature, 30 °C, and 37 °C)...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28195476/solvation-of-uracil-and-its-derivatives-by-dmso-a-dft-supported-1-h-nmr-and-13-c-nmr-study
#8
Dominika Antonina Kubica, Sergey Molchanov, Adam Gryff-Keller
(1)H NMR and (13)C NMR spectra of uracil, thymine, 5-hydroxymethyluracil, 5,6-dihydrouracil and 5,6-dihydrothymine in DMSO-d6 solutions have been measured. Additionally, molecular structures as well as NMR parameters of these compounds and their various solvates have been calculated using DFT B3LYP/6-311++G(2d,p) PCM(DMSO) method. The analysis of the chemical shift data for these compounds has shown that, indeed, in DMSO solutions they occur as equilibrium mixtures of free molecules and solvates in which solute and solvent molecules are joined by NH···O and/or OH···O hydrogen bonds...
February 14, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28195380/small-molecule-mixture-analysis-by-heteronuclear-nmr-under-spin-diffusion-conditions-in-the-viscous-dmso-water-solvent
#9
Pedro Lameiras, Solène Patis, Jouda Jakhlal, Stéphanie Castex, Pascale Clivio, Jean-Marc Nuzillard
Spin diffusion in NMR occurs for small and medium-sized molecules when their tumbling rate reduces in solution so that magnetization exchange by longitudinal cross relaxation becomes highly efficient. Composite DMSO-water viscous solvents were used for the first time to access the individual NMR spectra of mixture components in spin diffusion conditions. The easy handling and high dissolution power of [D6]DMSO/H2O offers a wide range of potential applications for polar and apolar mixture analysis. In addition to 2D 1H-1H NOESY and 1H-13C HSQC-NOESY, 1H-15N HSQC-NOESY, 1D and 2D 1H-19F HOESY experiments were set up to offer new ways to individualize molecules within a mixture...
February 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28193995/evaluating-the-effectiveness-of-gtm-1-rapamycin-and-carbamazepine-on-autophagy-and-alzheimer-disease
#10
Lijuan Zhang, Lina Wang, Run Wang, Yuan Gao, Haoyue Che, Yonghua Pan, Peng Fu
BACKGROUND This study was proposed to compare the efficacy and safety of GTM-1, Rapamycin (Rap), and Carbamazepine (CBZ) in managing Alzheimer disease (AD). The impact of the above mentioned therapeutic drugs on autophagy was also investigated in our study. MATERIAL AND METHODS Firstly, 3×Tg AD mice were randomly allocated into 4 groups (each group with 10 mice), in which AD mice were separately treated with dimethylsulfoxide (DMSO, vehicle group), GTM-1 (6 mg/kg), Rap (1 mg/kg), and CBZ (100 mg/kg). Then spatial memory and learning ability of mice was tested using the Morris water maze...
February 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28193424/a-hyperbranched-%C3%AE-d-glucan-with-compact-coil-conformation-from-lignosus-rhinocerotis-sclerotia
#11
Ting Hu, Qilin Huang, Kahing Wong, Hong Yang, Jingsi Gan, Yanru Li
An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC-MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1→3)-β-d-Glcp backbone with every three residues bearing a (1→6)-linked and hyperbranched side chain that contained three (1→6)-β-d-Glcp residues as secondary main chain and two terminal β-d-Glcp residues linked at O3. The degree of branching was 0.76 from GC-MS analysis, implying a highly branched structure for LRP...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28191569/benzimidazolylquinoxalines-novel-fluorophores-with-tuneable-sensitivity-to-solvent-effects
#12
Timur I Burganov, Nataliya A Zhukova, Vakhid A Mamedov, Christoph Bannwarth, Stefan Grimme, Sergey A Katsyuba
We report on the photophysical properties, conjugation, conformational behavior, intra- and intermolecular hydrogen bonds (HBs) of a series of novel fluorophores, consisting of 3-arylquinoxaline and benzimidazole moieties linked by a single CC bond. Computations employing density functional theory (DFT) reveal that conjugation between these moieties stabilizes syn-conformers with two HB centers located on the same side of the molecule. Anti-conformers form stronger intermolecular HBs with DMSO and DMF than syn-conformers, and this influences the energy gap between syn- and anti-forms, especially upon excitation of the molecules to the S1 state...
February 13, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28190562/a-combined-approach-to-early-detect-in-vitro-drug-induced-hemostatic-changes-in-preclinical-safety
#13
Myriam Defontis, Serge Côté, David Ledieu
Early detection of drug-induced alterations of hemostasis is challenging. Drugs can affect different components of the Virchow's triad and measurement of plasmatic coagulation times lacks sensitivity. New techniques for a more global assessment of the hemostasis are now available: the impedance platelet aggregometry, the thromboelastography and the thrombin generation measurement. The aim of this study was to evaluate three techniques (i.e.: Multiplate(®), TEG(®) and CAT) for the in vitro detection of the effect of a drug known to induce hemostatic alterations in a preclinical safety environment...
February 9, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28186696/evaluation-of-freeze-drying-and-cryopreservation-protocols-for-long-term-storage-of-biomaterials-based-on-decellularized-intestine
#14
Eloísa Díaz-Moreno, Daniel Durand-Herrera, Víctor Carriel, Miguel-Ángel Martín-Piedra, María-Del-Carmen Sánchez-Quevedo, Ingrid Garzón, Antonio Campos, Ricardo Fernández-Valadés, Miguel Alaminos
We evaluated the efficiency of several protocols to preserve the main components of decellularized tissue scaffolds for delayed use. Decellularized rat intestine scaffolds were generated by using SDS and triton X-100 and preserved for 3 months subjected to eight freeze-drying (F1 to F8) and 14 cryopreservation protocols (C1 to C14). Morphological analysis showed that cryopreservation tended to preserve the tissue morphostructure more efficiently than freeze-drying. Histological analysis showed that the content of proteoglycans and glycoproteins was efficiently preserved by most methods...
February 10, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28186202/inhibition-of-receptor-interacting-protein-kinase-1-with-necrostatin-1s-ameliorates-disease-progression-in-elastase-induced-mouse-abdominal-aortic-aneurysm-model
#15
Qiwei Wang, Ting Zhou, Zhenjie Liu, Jun Ren, Noel Phan, Kartik Gupta, Danielle M Stewart, Stephanie Morgan, Carmel Assa, K Craig Kent, Bo Liu
Abdominal aortic aneurysm (AAA) is a common aortic disease with a progressive nature. There is no approved pharmacological treatment to effectively slow aneurysm growth or prevent rupture. Necroptosis is a form of programmed necrosis that is regulated by receptor-interacting protein kinases (RIPs). We have recently demonstrated that the lack of RIP3 in mice prevented aneurysm formation. The goal of the current study is to test whether perturbing necroptosis affects progression of existing aneurysm using the RIP1 inhibitors Necrostatin-1 (Nec-1) and an optimized form of Nec-1, 7-Cl-O-Nec-1 (Nec-1s)...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28184930/resveratrol-enhances-the-radiosensitivity-of-nasopharyngeal-carcinoma-cells-by-downregulating-e2f1
#16
Yuhui Tan, Xianli Wei, Wenyin Zhang, Xiaolan Wang, Kun Wang, Biaoyan Du, Jianyong Xiao
Identification of safe, effective radiosensitizing agents is urgently needed to improve the outcome of radiotherapy in nasopharyngeal cancer (NPC). In this study, we assessed the ability of the polyphenol resveratrol to act as a radiosensitizer in vitro and in vivo. CNE-1 cells were treated with 50 µM resveratrol for 24 h, then irradiated. E2F transcription factor 1 (E2F1) was stably knocked down and overexpressed using lentiviruses. A xenograft model of NPC was established in nude mice using CNE-1 cells...
March 2017: Oncology Reports
https://www.readbyqxmd.com/read/28181812/transition-metal-free-selective-oxidative-c-sp-3-s-se-coupling-of-oxindoles-tetralone-and-arylacetamides-synthesis-of-unsymmetrical-organochalcogenides
#17
Ch Durga Prasad, Moh Sattar, Sangit Kumar
Transition-metal-free synthetic methods have been developed for the preparation of unsymmetrical diaryl and aryl alkyl chalcogenides: sulfones, sulfides, and selenides from the sp(3)-C-H bond of oxindole, tetralone, arylacetamide, and aryl chalcogenide precursors. Sulfones were obtained from sodium sulfinates using potassium iodide, tert-butyl hydroperoxide in DMSO, and acetic acid. Sulfides and selenides were prepared from diaryl disulfides or diselenides employing potassium tert-butoxide in DMSO. α-Tetralone underwent concomitant chalcogenation and aromatization resulting in 2-chalcogenyl-1-naphthols in one pot...
February 9, 2017: Organic Letters
https://www.readbyqxmd.com/read/28178884/improved-post-thaw-function-and-epigenetic-changes-in-mesenchymal-stromal-cells-cryopreserved-using-multicomponent-osmolyte-solutions
#18
Kathryn Pollock, Rebekah Samsonraj, Amel Dudakovic, Roman Thaler, Aron Stumbras, David McKenna, Peter Dosa, Andre van Wijnen, Allison Hubel
Current methods for freezing mesenchymal stromal cells (MSCs) result in poor post-thaw function, which limits the clinical utility of these cells. This investigation develops a novel approach to preserve MSCs using combinations of sugars, sugar alcohols and small molecule additives. MSCs frozen using these solutions exhibit improved post-thaw attachment and a more normal alignment of the actin cytoskeleton compared to cells exposed to dimethylsulfoxide (DMSO). Osteogenic and chondrogenic differentiation assays show that cells retain their mesenchymal lineage properties...
February 8, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28178225/poloxamer-based-thermoreversible-gel-for-topical-delivery-of-emodin-influence-of-p407-and-p188-on-solubility-of-emodin-and-its-application-in-cellular-activity-screening
#19
Eunmi Ban, Mijung Park, Seonghee Jeong, Taekhyun Kwon, Eun-Hee Kim, Kiwon Jung, Aeri Kim
Emodin is a component in a Chinese herb, Rheum officinale Baill, traditionally used for diabetes and anticancer. Its poor solubility is one of the major challenges to pharmaceutical scientists. We previously reported on thermoreversible gel formulations based on poloxamer for the topical delivery of emodin. The present study was to understand the effect of poloxamer type on emodin solubility and its application in cellular activity screening. Various gel formulations composed of poloxamer 407 (P407), poloxamer 188 (P188) and PEG400 were prepared and evaluated...
February 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28176384/the-effect-of-some-cryoprotectants-on-dromedary-camel-frozen-thawed-semen
#20
D A El-Badry, R H Mohamed, H A El-Metwally, T R Abo Al-Naga
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh-G), dimethyl formamide (DMF, Sh-DF), dimethyl sulfoxide (DMSO, Sh-DS) or ethylene glycol (EG, Sh-EG), all at 6% final concentration, and the samples were subjected to cryopreservation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
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