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microRNA 19

Kilian Friedrich, Carina Baumann, Andreas Wannhoff, Christian Rupp, Arianeb Mehrabi, Karl Heinz Weiss, Daniel N Gotthardt
BACKGROUND AND AIMS: The disease course of primary sclerosing cholangitis (PSC) is variable and difficult to predict. MicroRNA-122 (miR-122) is associated with various liver diseases. We investigated the value of miR-122 as a biomarker for the disease course of PSC. METHODS: We determined serum miR-122 levels in a long-term, prospective cohort of 114 PSC patients and a second validation cohort. RESULTS: Based on miR-122 levels, PSC patients were assigned to low or high level miR-122 groups...
June 2018: Journal of Gastrointestinal and Liver Diseases: JGLD
Duo Yu, Yunfeng Li, Zhihui Ming, Hongyong Wang, Zhuo Dong, Ling Qiu, Tiejun Wang
Background: Cervical cancer is one of the most common cancers in women worldwide. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. With the development of high-throughput sequencing technologies, circular RNA (circRNA) has been identified in an increasing number of diseases, especially cancers. It has been reported that circRNA can compete with microRNAs (miRNAs) to change the stability or translation of target RNAs, thus regulating gene expression at the transcriptional level...
2018: PeerJ
Koichi Sawaki, Mitsuro Kanda, Yasuhiro Kodera
Gastric cancer (GC) is the leading cause of cancer-related death worldwide. Despite recent advances in diagnosis and therapy, the prognosis of patients with GC is poor. Many patients have inoperable disease upon diagnosis or experience recurrent disease after curative gastrectomy. Unfortunately, tumor markers for GC, such as serum carcinoembryonic antigen and carbohydrate antigen 19-9, lack sufficient sensitivity and specificity. Therefore, effective biomarkers are required to detect early GC and to predict tumor recurrence and chemosensitivity...
June 14, 2018: Expert Review of Gastroenterology & Hepatology
Peng Zhang, Haifeng Gao, Qing Li, Xinlei Chen, Xifa Wu
The abnormal expression of microRNAs (miRNAs/miRs) has been observed in osteosarcoma (OS), and these differently expressed miRNAs contribute to the occurrence and development of OS by regulating various biological behaviours. Therefore, a comprehensive understanding of the detailed roles of aberrantly expressed miRNAs in OS progression may be favourable to the identification of promising therapeutic strategies for the treatment of patients with this malignancy. The present study demonstrated that miR‑660‑5p (miR‑660) expression was significantly upregulated in OS tissues and cell lines compared with that in normal adjacent tissues and normal human osteoblast hFOB1...
June 14, 2018: Molecular Medicine Reports
Zheng Li, Chao Jiang, Canhua Ye, Shibai Zhu, Xi Chen, William Kk Wu, Wenwei Qian
Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopedic disease. The lack of specific manifestations and effective diagnostic methods make it difficult for this disease to be diagnosed at early stages. Recent studies have shown that microRNAs (miRNA) participate in the development of steroid-induced ONFH, but there is limited research into the diagnostic use of circulating miRNAs. Blood samples from 23 human subjects (7 systemic lupus erythematosus (SLE) patients with steroid-induced ONFH; 7 SLE controls without ONFH; and 9 healthy controls) and 71 rats (19 with steroid-induced ONFH; 28 receiving steroids without ONFH; and 24 untreated controls) were collected to verify the abundance of changes of 6 previously identified ONFH-associated plasma miRNAs (miR-423-5p, miR-99a-5p, miR-10a-5p, miR-21-5p, miR-130a-3p and miR-6787-5p) by quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)...
2018: American Journal of Translational Research
Joanna Miskiewicz, Marta Szachniuk
A small non-coding molecule of microRNA (19⁻24 nt) controls almost every biological process, including cellular and physiological, of various organisms’ lives. The amount of microRNA (miRNA) produced within an organism is highly correlated to the organism’s key processes, and determines whether the system works properly or not. A crucial factor in plant biogenesis of miRNA is the Dicer Like 1 (DCL1) enzyme. Its responsibility is to perform the cleavages in the miRNA maturation process...
June 6, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jieshu Wu, Ye Jiang, Wanshuang Cao, Xiaoting Li, Chunfeng Xie, Shanshan Geng, Mingming Zhu, Zhaofeng Liang, Jianyun Zhu, Weiwei Zhu, Rui Wu, Xiao Ma, Cong Huang, Xue Yang, Shijia Wang, Caiyun Zhong
Breast cancer is the most common cancer among women worldwide. Butyl benzyl phthalate (BBP) is ubiquitous in human's environment, and is strongly linked to breast cancer development. microRNA (miRNA) is an important regulator of target genes. So far, no studies have been reported yet to reveal the action of miRNAs in BBP-mediated breast cancer cell proliferation. In this study, we showed that BBP induced proliferation of both ER(+) MCF-7 and ER(-) MDA-MB-231 breast cancer cells, proved by increased cell viability, transition of cell cycle from G1 to S phase, upregulation of PCNA and Cyclin D1, and downregulation of p21...
June 1, 2018: Toxicology Letters
Jing Li, Liang Hui, Qianyan Kang, Rong Li
Aberrant proliferation and migration of retinal pigment epithelium (RPE) cells contributes to the pathology of various ocular diseases. miR-27b has been reported to be crucial in the regulation of cell differentiation, proliferation, apoptosis, and migration. However, the role of miR-27b on RPE proliferation and migration remains largely unknown. Here the effect of miR-27b on ARPE-19 cells under platelet-derived growth factor (PDGF)-BB stimulation was explored. In this study, we found that the expression level of miR-27b was significantly reduced in ARPE-19 cells under PDGF-BB stimulation...
May 24, 2018: Pathology, Research and Practice
Xue-Jia Lin, Jian-Hong Fang, Xiao-Jing Yang, Chong Zhang, Yunfei Yuan, Limin Zheng, Shi-Mei Zhuang
We previously found that 19 microRNAs (miRNAs) significantly increased in the sera of hepatocellular carcinoma (HCC) patients. Here, we evaluated whether these miRNAs were secreted by HCC cells and contributed to tumor angiogenesis. High level of miR-210-3p (miR-210) was detected in the exosomes isolated from the sera of HCC patients and the conditioned media of hepatoma cells. Higher miR-210 level in serum was correlated with higher microvessel density in HCC tissues. Moreover, the HCC cell-secreted exosomes promoted in vitro tubulogenesis of endothelial cells, which was strengthened by overexpressing miR-210 in HCC cells but was attenuated by repressing miR-210 or DROSHA in HCC cells...
June 1, 2018: Molecular Therapy. Nucleic Acids
Ander Anasagasti, Maitane Ezquerra-Inchausti, Olatz Barandika, Maider Muñoz-Culla, María M Caffarel, David Otaegui, Adolfo López de Munain, Javier Ruiz-Ederra
Purpose: The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods: miRNAs-mRNA interaction networks were generated for analysis of biological pathways involved in retinal degeneration. Results: Of more than 1900 miRNAs analyzed, we selected 19 miRNAs on the basis of (1) a significant differential expression in rd10 retinas compared with control samples and (2) an inverse expression relationship with predicted mRNA targets involved in biological pathways relevant to retinal biology and/or degeneration...
May 1, 2018: Investigative Ophthalmology & Visual Science
Christoph Sippl, Ralf Ketter, Lisa Bohr, Yoo Jin Kim, Markus List, Joachim Oertel, Steffi Urbschat
BACKGROUND: Standard therapeutic protocols for glioblastoma, the most aggressive type of brain cancer, include surgery followed by chemoradiotherapy. Additionally, carmustine-eluting wafers can be implanted locally into the resection cavity. OBJECTIVE: To evaluate microRNA (miRNA)-181d as a prognostic marker of responses to carmustine wafer implantation. METHODS: A total of 80 glioblastoma patients (40/group) were included in a matched pair analysis...
May 26, 2018: Neurosurgery
J Kováčová, J Juráček, A Poprach, T Büchler, J Kopecký, O Fiala, M Svoboda, M Lojová, O Slabý
BACKGROUND: Renal cell carcinoma (RCC) accounts for 2-3% of all malignant tumours. Metastatic RCC (mRCC) is commonly treated with tyrosine kinase inhibitors (TKI). Effective TKIs administration can be achieved only by accurate prediction of therapeutical response. Therefore, the aim of this study was to analyse papers concerning predictive potential of microRNA (miRNA). MATERIAL AND METHODS: We chose seven candidate miRNAs and analysed their expression on 44 patients divided into cohort with poor and good response to sunitinib treatment...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
A Kopková, J Šána, M Večeřa, D Knoflíčková, M Smrčka, O Slabý, P Fadrus
BACKGROUNDS: Deregulated levels of miRNAs, short noncoding RNAs associated with pathogenesis of many diseases, have been observed in cerebrospinal fluid (CSF). Therefore, the analysis of CSF miRNAs in patients affected by tumors of central nervous system (CNS) might help to develop new diagnostic platform enabling more precise diagnosis. Thus, in our study we tried to optimize methodical approaches to be used for miRNA detection as RNA isolation and selection of suitable technology for global high-throughput miRNA profiling...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
J Juráček, M Staník, B Peltanová, S Adamcová, J Doležel, M Fedorko, L Radová, O Slabý
BACKGROUND: Currently, there are no urinary-based tumour markers with sufficient sensitivity and specificity to replace cystoscopy in the detection of bladder cancer (BCA). Urinary microRNAs are emerging as clinically useful class of biomarkers for early and non-invasive detection of urologic malignancies. PATIENTS AND METHODS: In this study, 155 patients with BCA and 83 healthy controls were enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays and candidate miRNAs further validated in independent cohort using specific TaqMan assays and quantitative real-time polymerase chain reaction method...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
P Ahmad, M Slavík, J Šána, M Hermanová, P Šlampa, P Smilek, T Kazda, O Slabý
BACKGROUND: Radiotherapy plays a key role in the treatment of squamous cell head and neck cancers (HNSCC). The effectivity of radiation therapy is often limited by radioresistance of these tumours. microRNAs (miRNAs) are endogenous, evolutionary conserved, small non-coding RNAs involved in regulation of cellular processes associated with radioresistance. The objective of this study was to identify miRNA profile enabling to predict the radiation treatment outcomes in HNSCC patients. MATERIAL AND METHODS: The retrospective study included HNSCC patients who underwent a definitive radiotherapy...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
Sandra Franco, Daniela Buccione, Raquel Pluvinet, Beatriz Mothe, Lidia Ruiz, Maria Nevot, Ana Jordan-Paiz, Laia Ramos, Susanna Aussó, Rosa M Morillas, Lauro Sumoy, Miguel Angel Martinez, Cristina Tural
Human immunodeficiency virus type 1 (HIV-1)-induced inflammation and/or long-term antiretroviral drug toxicity may contribute to the evolution of liver disease. We investigated circulating plasma microRNAs (miRNAs) as potential biomarkers of liver injury in patients mono-infected with HIV-1. We performed large-scale deep sequencing analyses of small RNA level on plasma samples from patients with HIV-1 mono-infection that had elevated or normal levels of alanine aminotransferase (ALT) or focal nodular hyperplasia (FNH)...
May 25, 2018: Antiviral Research
Ji Eun Kim, Jung Seop Eom, Won-Young Kim, Eun Jung Jo, Jeongha Mok, Kwangha Lee, Ki Uk Kim, Hye-Kyung Park, Min Ki Lee, Mi-Hyun Kim
BACKGROUND: Low-dose computed tomography can identify smaller nodules more often than chest radiography in lung screening. However, complications from invasive diagnostic procedures performed to detect nodules are common. Exosomes contain a diverse array of biomolecules that reflect the biological state of the cell from which they are released. The aim of this study was to investigate the diagnostic value of bronchoalveolar lavage (BAL) fluid exosomal microRNAs (miRNAs) for early-stage lung adenocarcinoma...
May 28, 2018: Thoracic Cancer
Ping Lin, Lijuan Zhu, Wenjing Sun, Zhengkai Yang, Hui Sun, Dong Li, Rongjun Cui, Xiulan Zheng, Xiaoguang Yu
MicroRNAs (miRNAs/miRs), which are endogenous non-coding single-stranded RNAs 19-25 nucleotides in length, regulate gene expression by blocking translation or transcription repression. The present study revealed that miR-3160-5p was widely expressed in prostate cancer cells by reverse transcription-quantitative polymerase chain reaction. There was a negative association between the expression of miR-3160-5p and F-box and WD repeat domain containing 8 (Fbxw8) in prostate cancer DU145 cells. A luciferase activity assay was used to verify that Fbxw8 is the target of miR-3160-5p...
June 2018: Oncology Letters
Ning Yang, Ling Wang, Jun Liu, Li Liu, Jiangbo Huang, Xian Chen, Zhigang Luo
The present study investigated the molecular mechanism by which microRNA-206 (miR-206) targets Annexin A2 (ANXA2) expression and inhibits the invasion and metastasis of prostatic cancer cells through regulation of the epithelial-mesenchymal transition (EMT). Using bioinformatics analysis, miR-206 was identified as the most promising candidate miRNA that targeted ANXA2 . Prostate tissue specimens from 60 patients with prostate cancer, 30 patients with metastatic prostate cancer and 20 patients with benign prostatic hyperplasia (BPH) were examined for ANXA2 protein expression by immunohistochemistry and western blotting and for miR-206 expression by reverse transcription-quantitative polymerase chain reaction...
June 2018: Oncology Letters
Hongchao Han, Lisheng Wang, Jie Xu, Aikun Wang
MicroRNAs (miRNA/miRs) are small, non-coding RNA molecules (19-25 nucleotides in length), which function to regulate gene expression. It has been reported that miR-128 serves an important role in regulating cancer cell growth; increasing evidence has indicated that the expression of miR-128 is decreased in pancreatic cancer (PC) cells. However, the specific mechanisms of miR-128 in regulating PC cell growth are unclear. In the present study, it was confirmed that the expression of miR-128 was significantly decreased within PC tissues compared with adjacent normal tissues via reverse transcription-quantitative polymerase chain reaction analysis...
June 2018: Experimental and Therapeutic Medicine
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