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https://www.readbyqxmd.com/read/27911275/microrna-25-targets-pkc%C3%AE-and-protects-osteoblastic-cells-from-dexamethasone-via-activating-ampk-signaling
#1
Jian-Bo Fan, Wei Liu, Xin-Hui Zhu, Hong Yi, Sheng-Yu Cui, Jian-Ning Zhao, Zhi-Ming Cui
AMP-activated protein kinase (AMPK) activation could protect osteoblasts from dexamethasone (Dex). This study aims to provoke AMPK activation via microRNA downregulation of its negative regulator protein kinase C ζ (PKCζ). Results show that microRNA-25-5p (miR-25-5p) targets PKCζ's 3' untranslated regions (UTRs). Forced-expression of miR-25 downregulated PKCζ and activated AMPK in human osteoblastic cells (OB-6 and hFOB1.19 lines), which thereafter protected cells from Dex. Reversely, expression of antagomiR-25, the miR-25 inhibitor, upregulated PKCζ and inhibited AMPK activation, exacerbating Dex damages...
November 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27903911/mirtar2go-a-novel-rule-based-model-learning-method-for-cell-line-specific-microrna-target-prediction-that-integrates-ago2-clip-seq-and-validated-microrna-target-interaction-data
#2
Alireza Ahadi, Gaurav Sablok, Gyorgy Hutvagner
MicroRNAs (miRNAs) are ∼19-22 nucleotides (nt) long regulatory RNAs that regulate gene expression by recognizing and binding to complementary sequences on mRNAs. The key step in revealing the function of a miRNA, is the identification of miRNA target genes. Recent biochemical advances including PAR-CLIP and HITS-CLIP allow for improved miRNA target predictions and are widely used to validate miRNA targets. Here, we present miRTar2GO, which is a model, trained on the common rules of miRNA-target interactions, Argonaute (Ago) CLIP-Seq data and experimentally validated miRNA target interactions...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27895662/muscle-enriched-micrornas-isolated-from-whole-blood-are-regulated-by-exercise-and-are-potential-biomarkers-of-cardiorespiratory-fitness
#3
Joshua Denham, Priscilla R Prestes
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally. Evidence indicating miRNAs influence exercise-induced health and performance adaptations is mounting. Circulating miRNAs are responsible for intercellular communication and could serve as biomarkers for disease and exercise-related traits. Such biomarkers would contribute to exercise screening, monitoring, and the development of personalized exercise prescription. Accordingly, we investigated the impact of long-term strenuous aerobic exercise training and a single bout of maximal aerobic exercise on five muscle-enriched miRNAs implicated in exercise adaptations (miR-1, miR-133a, miR-181a, miR-486, and miR-494)...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27871470/the-function-of-mir-519d-in-cell-migration-invasion-and-proliferation-suggests-a-role-in-early-placentation
#4
REVIEW
Lan Xie, Yoel Sadovsky
The processes of proliferation, migration, and invasion of extravillous trophoblasts are critical for placental implantation and early development, and directly influence pregnancy outcome. Dysregulation of these processes has been associated with placental dysfunction, implicated in clinical conditions such as preeclampsia and placental accreta. Among diverse microRNA (miRNA) species that are expressed in placental trophoblasts, members of the chromosome 19 miRNA cluster (C19MC) stand out in their nearly exclusive expression in the placenta...
December 2016: Placenta
https://www.readbyqxmd.com/read/27855604/novel-microrna-signature-to-differentiate-ulcerative-colitis-from-crohn-disease-a-genome-wide-study-using-next-generation-sequencing
#5
Jingmei Lin, Xinjun Zhang, Zijin Zhao, Noah Welker, Yong Li, Yunlong Liu, Mary Bronner
BACKGROUND: The diagnosis of ulcerative colitis (UC) or Crohn disease (CD) can be challenging given the overlapping features. Knowledge of microRNAs in IBD has expanded recently and supports that microRNAs play an important role. This study aimed to identify novel microRNA biomarkers through comprehensive genome-wide sequencing to distinguish UC from CD. DESIGN: Illumina next generation sequencing was performed on nondysplastic fresh-frozen colonic mucosa of the distal-most colectomy from 19 patients (10 UC and 9 CD) and 18 patients with diverticular disease serving as controls...
November 17, 2016: MicroRNA
https://www.readbyqxmd.com/read/27853906/downregulation-of-estrogen-receptor-and-modulation-of-growth-of-breast-cancer-cell-lines-mediated-by-paracrine-stromal-cell-signals
#6
J Huang, P Woods, D Normolle, J P Goff, P V Benos, C J Stehle, R A Steinman
PURPOSE: Breast cancers have a poorer prognosis if estrogen receptor expression was lost during recurrence. It is unclear whether this conversion is cell autonomous or whether it can be promoted by the microenvironment during cancer dormancy. We explored the ability of marrow-derived stromal cell lines to arrest co-cultured breast cancer cells and suppress estrogen receptor alpha (ER) expression during arrest, facilitating the emergence of estrogen-independent breast cancer clones. METHODS: Cancer cell growth, ER protein, microRNA, and mRNA levels were measured in breast cancer cell lines exposed to conditioned medium from marrow stromal lines in the presence and absence of estrogen and of signaling pathway modulators...
November 16, 2016: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/27824863/demethylation-of-microrna-124a-genes-attenuated-proliferation-of-rheumatoid-arthritis-derived-fibroblast-like-synoviocytes-and-synthesis-of-tumor-necrosis-factor-%C3%AE
#7
Qiao Zhou, Li Long, Ting Zhou, Juan Tian, Bin Zhou
OBJECTIVE: To examine the impact of 5-Aza-2'-deoxycytidine (5-AzadC) on methylation status of miR-124a genes in rheumatoid arthritis (RA) associated fibroblast-like synoviocytes (FLS) and its effect on RA-FLS proliferation and TNF-α expression. MATERIALS AND METHODS: FLS were isolated from seven RA-derived synovial tissues and cultured in vitro. The expression of miR-124a was measured by real time quantitative polymerase chain reaction (PCR) in FLS with or without 5-AzadC treatment...
2016: PloS One
https://www.readbyqxmd.com/read/27823999/microrna-210-plays-a-critical-role-in-the-angiogenic-effect-of-isoprenaline-on-human-umbilical-vein-endothelial-cells-via-regulation-of-noncoding-rnas
#8
You-You Yan, Zhi-Hui Wang, Lei Zhao, Dan-Dan Song, Chao Qi, Lu-Lu Liu, Jun-Nan Wang
BACKGROUND: β-adrenoceptors play a crucial regulatory role in blood vessel endothelial cells. Isoprenaline (ISO, a β-adrenergic agonist) has been reported to promote angiogenesis through upregulation of vascular endothelial growth factor (VEGF) expression; however, the underlying mechanism remains to be investigated. It is widely accepted that certain noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), can regulate endothelial cell behavior, including their involvement in angiogenesis...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27822220/identification-of-micrornas-actively-involved-in-fatty-acid-biosynthesis-in-developing-brassica-napus-seeds-using-high-throughput-sequencing
#9
Jia Wang, Hongju Jian, Tengyue Wang, Lijuan Wei, Jiana Li, Chao Li, Liezhao Liu
Seed development has a critical role during the spermatophyte life cycle. In Brassica napus, a major oil crop, fatty acids are synthesized and stored in specific tissues during embryogenesis, and understanding the molecular mechanism underlying fatty acid biosynthesis during seed development is an important research goal. In this study, we constructed three small RNA libraries from early seeds at 14, 21, and 28 days after flowering (DAF) and used high-throughput sequencing to examine microRNA (miRNA) expression...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27819716/association-of-genetic-variations-in-mir-146a-rs2910164-and-mir-149-rs11614913-with-the-development-of-classic-kaposi-sarcoma
#10
H Yang, Q L Lu, X J Wu, H Y Ma, Y Y Qu, D Z Zhang, X M Pu
Classic Kaposi sarcoma is a type of vascular proliferative inflammatory disease. Previous studies have reported significant associations between microRNAs expression and the development of classic Kaposi sarcoma. Here, we conducted a case-control study to investigate the association between miR-146a and miR-149 genetic polymorphisms and risk of classic Kaposi sarcoma in a Chinese population. Both classic Kaposi sarcoma patients and healthy controls were recruited between December 2013 and October 2015. Genotyping of miR-146a and miR-149 was performed by polymerase chain reaction-coupled with restriction fragment length polymorphism...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27818919/micrornas-in-idiopathic-pulmonary-fibrosis-involvement-in-pathogenesis-and-potential-use-in-diagnosis-and-therapeutics
#11
REVIEW
Huimin Li, Xiaoguang Zhao, Hongli Shan, Haihai Liang
MicroRNAs (miRNAs) are a class of phylogenetically conserved, non-coding short RNAs, 19-22 nt in length which suppress protein expression through base-pairing with the 3'-untranslated region of target mRNAs. miRNAs have been found to participate in cell proliferation, differentiation and apoptosis. Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and high lethality fibrotic lung disease for which currently there is no effective treatment. Some miRNAs have been reported to be involved in the pathogenesis of pulmonary fibrosis...
November 2016: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/27808368/association-between-polymorphisms-in-the-promoter-region-of-pri-mir-34b-c-and-risk-of-hepatocellular-carcinoma
#12
L L Chen, Y Shen, J B Zhang, S Wang, T Jiang, M Q Zheng, Z J Zheng, C X Chen
Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths worldwide. MicroRNA-34 (miR-34) gene plays a key role in altering the apoptotic cycle and pathways of downstream cells, and therefore influences carcinogenesis. In this case-control study, we assessed the role of the pri-miR-34b/c rs4938723 polymorphism in HCC risk. The pri-miR-34b/c polymorphic genotype was determined in 286 patients with HCC and 572 controls using polymerase chain reaction-restriction fragment length polymorphism. The male gender (X(2) = 12...
October 5, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27804865/microrna-decay-refining-microrna-regulatory-activity
#13
Geneviève Pépin, Michael Paul Gantier
MicroRNAs (miRNAs) are short 19-25 nucleotide RNA molecules that impact on most biological processes by regulating the efficiency of messenger RNA (mRNA) translation. To date, most research activity has been focused on the control of miRNA expression and its functional consequences. Nonetheless, much remains unknown about the mechanisms affecting the level of specific miRNAs in the cell, a critical feature impacting on their regulatory activity. This review focuses on the factors that regulate the abundance of miRNAs, including synthesis, post-transcriptional modifications, nucleases, target binding, and secretion...
October 27, 2016: MicroRNA
https://www.readbyqxmd.com/read/27801427/developmental-changes-in-barley-microrna-expression-profiles-coupled-with-mirna-target-analysis
#14
Andrzej Pacak, Katarzyna Kruszka, Aleksandra Swida-Barteczka, Przemyslaw Nuc, Wojciech Karlowski, Artur Jarmolowski, Zofia Szweykowska-Kulinska
MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development...
November 2, 2016: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/27796698/microrna-155-induced-t-lymphocyte-subgroup-drifting-in-iga-nephropathy
#15
Lichuan Yang, XiaoYan Zhang, Wei Peng, Mian Wei, Wei Qin
BACKGROUND: MicroRNA-155 (miR-155) is an important immune regulator of T lymphocyte subgroup balance and function. This study was performed to explore the relationships between miR-155 expression in peripheral blood mononuclear cells (PBMCs), T lymphocyte subgroups, T lymphocyte regulators, and the clinical manifestations of IgA nephropathy (IgAN) patients. METHODS: Sixty biopsy-proven IgAN patients and 25 healthy controls were included in this study. The expression of miRNAs in PBMCs was determined using a microRNA microarray and real-time RT-PCR...
October 31, 2016: International Urology and Nephrology
https://www.readbyqxmd.com/read/27781291/expressed-microrna-associated-with-high-rate-of-egg-production-in-chicken-ovarian-follicles
#16
N Wu, U Gaur, Q Zhu, B Chen, Z Xu, X Zhao, M Yang, D Li
MicroRNA (miRNA) is a highly conserved class of small noncoding RNA about 19-24 nucleotides in length that function in a specific manner to post-transcriptionally regulate gene expression in organisms. Tissue miRNA expression studies have discovered a myriad of functions for miRNAs in various aspects, but a role for miRNAs in chicken ovarian tissue at 300 days of age has not hitherto been reported. In this study, we performed the first miRNA analysis of ovarian tissues in chickens with low and high rates of egg production using high-throughput sequencing...
October 26, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27780544/isolation-of-human-trophoblastic-extracellular-vesicles-and-characterization-of-their-cargo-and-antiviral-activity
#17
Yingshi Ouyang, Avraham Bayer, Tianjiao Chu, Vladimir A Tyurin, Valerian E Kagan, Adrian E Morelli, Carolyn B Coyne, Yoel Sadovsky
INTRODUCTION: Primary human trophoblasts release a repertoire of extracellular vesicles (EVs). Among them are nano-sized exosomes, which we found to suppress the replication of a wide range of diverse viruses. These exosomes contain trophoblastic microRNAs (miRNAs) that are expressed from the chromosome 19 miRNA cluster and exhibit antiviral properties. Here, we report our investigation of the cargo of placental EVs, focusing on the composition and the antiviral properties of exosomes, microvesicles, and apoptotic blebs...
November 2016: Placenta
https://www.readbyqxmd.com/read/27777199/-expression-of-microrna-155-in-papillary-thyroid-carcinoma-and-its-clinical-significance
#18
You-Zhi Zhu, Ke Zheng, Hui-Hao Zhang, Ling Chen, Kun-Lin Wu, Cai-Hong Ren, Zong-Cai Wang, Ling-Jun Kong, Wei-Hong Ruan, Xiang-Jin Chen
OBJECTIVE: To investigate the correlation of microRNA-155 (miR-155) expression with clinicopathological features of patients with papillary thyroid carcinoma (PTC) and explore the value of miR-155 in prognostic assessment of PTC. METHODS: We collected 86 pairs of fresh PTC and adjacent tissues to examine the expression of miR-155 using fluorescent quantitative PCR. miR-155 expressions in the tissues were analyzed in relation to the clinicopathological features of the patients...
October 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27775664/a-comprehensive-microrna-expression-profile-of-liver-and-lung-metastases-of-colorectal-cancer-with-their-corresponding-host-tissue-and-its-prognostic-impact-on-survival
#19
Mathieu Pecqueux, Isabell Liebetrau, Wiebke Werft, Hendrik Dienemann, Thomas Muley, Joachim Pfannschmidt, Benjamin Müssle, Nuh Rahbari, Sebastian Schölch, Markus W Büchler, Jürgen Weitz, Christoph Reissfelder, Christoph Kahlert
MicroRNAs are small non-coding RNAs with a length of 18-25 nucleotides. They can regulate tumor invasion and metastasis by changing the expression and translation of their target mRNAs. Their expression is substantially altered in colorectal cancer cells as well as in the adjacent tumor-associated stroma. Both of these compartments have a mutual influence on tumor progression. In the development of metastases, cancer cells initially interact with the host tissue. Therefore, compartment-specific expression signatures of these three locations-tumor, associated stroma, and host tissue-can provide new insights into the complex tumor biology of colorectal cancer...
October 21, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27765331/altered-expression-of-interleukin-17a-and-its-targeting-micrornas-in-oral-lichen-planus-a-pilot-study
#20
Zhengyu Shen, Chenping Zhang, Zengtong Zhou, Wei Liu, Linjun Shi, Xuemin Shen
OBJECTIVE: To investigate the potential role of interleukin (IL)-17A and its targeting microRNAs (miRNAs) in oral lichen planus (OLP). STUDY DESIGN: We compared the IL-17A expression levels in biopsies from patients with OLP (n = 50) against normal controls (n = 19) by using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot analyses, identified the targeting miRNAs of IL-17A, and examined the miRNA expression levels in OLP...
November 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
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