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https://www.readbyqxmd.com/read/29674246/a-microrna-expression-signature-of-the-postprandial-state-in-response-to-a-high-saturated-fat-challenge
#1
Sergio Lopez, Beatriz Bermudez, Sergio Montserrat-de la Paz, Rocio Abia, Francisco J G Muriana
The postprandial hypertriglyceridemia is an important and largely silent disturbance involved in the genesis of numerous pathological conditions. Exaggerated and prolonged states of postprandial hypertriglyceridemia are frequently related to the ingestion of meals enriched in saturated fatty acids (SFAs). MicroRNAs are noncoding RNAs that function as gene regulators and play significant roles in both health and disease. However, differential miRNA expression between fasting and postprandial states has never been elucidated...
March 18, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29671027/comparison-of-commercial-exosome-isolation-kits-for-circulating-exosomal-microrna-profiling
#2
Meng Ding, Cheng Wang, Xiaolan Lu, Cuiping Zhang, Zhen Zhou, Xi Chen, Chen-Yu Zhang, Ke Zen, Chunni Zhang
Circulating exosomal microRNAs (miRNAs) are valuable biomarker candidates; however, information on the characterization and mutual agreement of commercial kits for circulating exosomal miRNA profiling is scarce. Here, we analyzed the advantages and weaknesses of four commonly used commercial kits for exosomal miRNA profiling and their application to the sample of serum and/or plasma, respectively. NanoSight and Western blotting were conducted to evaluate the efficiency and purity of the isolated exosomes. In our conditions, the size distribution of the isolated particles was appropriate (40-150 nm), and ExoQuick™ Exosome Precipitation Solution (EXQ) generated a relatively high yield of exosomes...
April 18, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29660336/microrna-155-inversely-correlates-with-esophageal-cancer-progression-through-regulating-tumor-associated-macrophage-fgf2-expression
#3
Peng Wang, Li-Juan Xu, Jie-Jie Qin, Lu Zhang, Gui-Hua Zhuang
Esophageal cancer (EC) is one of the most common malignancies with high incidence and mortality. Tumor-associated macrophages (TAMs) in the tumor microenvironment have been linked to the accelerated tumor progression. MicroRNAs (miR) are 19-25 nucleotide-long, noncoding RNA molecules, functioning as modulators of gene expression, and mediate a variety of biological functions, including tumor growth. In the present study, the effects and molecular mechanism of miR-155 in TAMs isolated from EC were explored. The expression of miR-155 and fibroblast growth factor-2 (FGF2) in EC tissues and cell lines were analyzed using reverse transcription-quantitative PCR (qRT-PCR) and western blot assays...
April 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29657293/mir-146-and-mir-155-two-key-modulators-of-immune-response-and-tumor-development
#4
REVIEW
Ugo Testa, Elvira Pelosi, Germana Castelli, Catherine Labbaye
MicroRNAs (miRNAs or miRs) are a class of evolutionarily-conserved small, regulatory non-coding RNAs, 19-3 nucleotides in length, that negatively regulate protein coding gene transcripts' expression. miR-146 (146a and 146b) and miR-155 are among the first and most studied miRs for their multiple roles in the control of the innate and adaptive immune processes and for their deregulation and oncogenic role in some tumors. In the present review, we have focused on the recent acquisitions about the key role played by miR-146a, miR-146b and miR-155 in the control of the immune system and in myeloid tumorigenesis...
June 26, 2017: Non-Coding RNA
https://www.readbyqxmd.com/read/29642855/mir-30a-5p-confers-cisplatin-resistance-by-regulating-igf1r-expression-in-melanoma-cells
#5
Yuxia Li, Jie Zhang, Yajing Liu, Bingyue Zhang, Fubo Zhong, Shubin Wang, Zhengyu Fang
BACKGROUND: Melanoma is notoriously resistant to all current modalities of cancer therapies including chemotherapy. In recent years, microRNAs (miRNAs) have emerged as molecular regulators in the development and progression of melanoma. However, the relationship between microRNA and chemo-resistance of melanoma is little known. In present study, we aimed to investigate the miRNAs related to cisplatin-resistance in melanoma cells. METHODS: After cisplatin (DDP) resistant melanoma cells (M8/DDP and SK-Mel-19/DDP) were established in-vitro, high-throughput screening of differentially expressed miRNAs between resistant cells and parental cells were performed...
April 11, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29626470/exosomal-mir-675-from-metastatic-osteosarcoma-promotes-cell-migration-and-invasion-by-targeting-caln1
#6
Liangzhi Gong, Qiyuan Bao, Chuanzhen Hu, Jun Wang, Qi Zhou, Li Wei, Lei Tong, Weibin Zhang, Yuhui Shen
Exosomal microRNAs(miRNAs) transfer from tumor to stromal cells is reportedly associated with cancer progression and metastasis in various epithelial cancers. However, the role of exosomal miRNA in the metastasis of osteosarcoma(OS) -the most common bone malignancy-still largely remains unknown. In this study, we purified exosomes with a median size close to 100 nm from cell culture media as well as patient serum, and proved that exosomes derived from the metastatic, but not the non-metastatic OS cells increase the migration and invasion of non-malignant fibroblast cells (hFOB1...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29622762/combination-of-circulating-mirna-320a-b-and-d-dimer-improves-diagnostic-accuracy-in-deep-vein-thrombosis-patients
#7
Zhiyun Jiang, Junfen Ma, Qian Wang, Fan Wu, Jiedan Ping, Liang Ming
BACKGROUND D-dimer tests have been widely used to rule-out deep venous thrombosis (DVT), but with low specificity. Circulating microRNAs (miRNAs) are novel promising biomarkers in diverse diseases. The purpose of our study was to identify the diagnostic abilities of circulating miRNA-320a/b and to assess their correlation with plasma D-dimer in DVT and post-thrombotic syndrome (PTS) patients. MATERIAL AND METHODS Plasma samples were taken from 30 DVT patients, 30 PTS patients, and 30 age- and sex-matched healthy volunteers...
April 6, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29618948/microrna-338-3p-inhibits-proliferation-migration-invasion-and-emt-in-osteosarcoma-cells-by-targeting-activator-of-90-kda-heat-shock-protein-atpase-homolog-1
#8
Riliang Cao, Jianli Shao, Yabin Hu, Liang Wang, Zhizhong Li, Guodong Sun, Xiaoliang Gao
Background: Osteosarcoma (OS) is a rare, malignant bone tumor that primarily affects adolescents and has a high degree of malignancy and high incidence of recurrence and metastasis. Our study aimed to explore the role of miR-338-3p in OS cells. Methods: qRT-qPCR was performed to quantify miR-338-3p expression levels in OS tissue samples and in three common OS cell lines. MG-63 and Saos2 cells were separately transfected with miR-338-3p or NC mimics and miR-338-3p expression levels was determined by qRT-PCR...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29595698/identification-of-key-genes-and-mirnas-associated-with-carotid-atherosclerosis-based-on-mrna-seq-data
#9
Zhanglin Mao, Fen Wu, Yunfeng Shan
This study was aimed to explore the crucial genes and microRNAs (miRNAs) associated with the carotid atherosclerosis (CA).Two public datasets GSE28829 and GSE43292 were obtained from Gene Expression Omnibus databases to analyze the differentially expressed genes (DEGs) between primary and advanced atherosclerotic plaque tissues. The Gene Ontology (GO) terms, pathways, and protein-protein interactions (PPIs) of these DEGs were analyzed. miRNAs and transcription factor (TF) were predicted.A total of 112 upregulated and 179 downregulated intersection DEGs were identified between 2 datasets...
March 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29575671/rituximab-may-cause-increased-hepatitis-c-virus-viremia-in-rheumatoid-arthritis-patients-through-declining-exosomal-microrna-155
#10
Tsai-Ling Liao, Shie-Liang Hsieh, Yi-Ming Chen, Hsin-Hua Chen, Hung-Jen Liu, Hsiu-Chin Lee, Der-Yuan Chen
OBJECTIVE: Several studies observed that rituximab (RTX) may enhance hepatitis C virus (HCV) viral activity. MicroRNAs (miRNAs) have been implicated in modulating host immune response in HCV infection and then shuttled by the exosomes to exert biological functions. But the role of exosomal miRNAs in RTX-related HCV activity enhancement remains unclear. METHODS: The association between RTX and increased HCV activity was examined using an in vitro cell-based assay...
March 25, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29568931/mir-590-3p-mediates-melatonin-induced-cell-apoptosis-by-targeting-septin-7-in-the-human-osteoblast-cell-line-hfob-1-19
#11
Xiaotong Meng, Yue Zhu, Lin Tao, Sichao Zhao, Shui Qiu
The present study aimed to investigate the association between septin 7 (SEPT7) and melatonin-induced apoptosis in the human fetal osteoblastic cell line hFOB 1.19. MicroRNA (miR)‑590‑3p was identified by identifying overlapping miRNAs that target SEPT7, across different databases (miRDB, DIANA and Targetscan). Apoptosis was assessed via flow cytometric analysis. Small interfering RNA of SEPT7 and a miR‑590‑3p inhibitor were used for gene silencing and the efficiency was assessed by reverse transcription‑quantitative polymerase chain reaction...
March 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29568877/microrna%C3%A2-22-inhibits-the-proliferation-and-migration-and-increases-the-cisplatin-sensitivity-of-osteosarcoma-cells
#12
Xiang Zhou, Dimple Natino, Xu Zhai, Zhongyang Gao, Xijing He
Osteosarcoma (OS) is the major type of primary bone tumor and is associated with a poor prognosis due to chemotherapy resistance. Accumulating evidence indicates that microRNAs (miRNAs/miRs) may influence the tumor progression of OS and cell sensitivity to chemotherapy. In the present study, a total of 7 patients with OS and 7 healthy volunteers were recruited. Reverse transcription‑quantitative polymerase chain reaction and ELISA were performed to determine the expression of miRNAs and mRNAs in the serum of participants...
March 20, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29568201/three-microrna-signature-identified-by-bioinformatics-analysis-predicts-prognosis-of-gastric-cancer-patients
#13
Cheng Zhang, Chun-Dong Zhang, Ming-Hui Ma, Dong-Qiu Dai
AIM: To identify multiple microRNAs (miRNAs) for predicting the prognosis of gastric cancer (GC) patients by bioinformatics analysis. METHODS: The original microarray dataset GSE93415, which included 20 GC and 20 tumor adjacent normal gastric mucosal tissues, was downloaded from the Gene Expression Omnibus database and used for screening differentially expressed miRNAs (DEMs). The cut-off criteria were P < 0.05 and fold change > 2.0. In addition, we acquired the miRNA expression profiles and clinical information of 361 GC patients from The Cancer Genome Atlas database to assess the prognostic role of the DEMs...
March 21, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29567311/conditional-regulation-of-gene-expression-by-ligand-induced-occlusion-of-a-microrna-target-sequence
#14
Huihui Mou, Guocai Zhong, Matthew R Gardner, Haimin Wang, Yi-Wen Wang, Dechun Cheng, Michael Farzan
RNA switches that modulate gene expression with small molecules have a number of scientific and clinical applications. Here, we describe a novel class of small regulatory on switches based on the ability of a ligand-bound aptamer to promote stem formation between a microRNA target sequence (miR-T) and a complementary competing strand. Two on switch architectures employing this basic concept were evaluated, differing in the location of a tetracycline aptamer and the region of a miR-21 target sequence (miR-21-T) masked by its competing strand...
February 27, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29564929/hepatotoxicity-after-paracetamol-overdose-in-a-patient-with-cystic-fibrosis-despite-early-acetylcysteine-and-utility-of-microrna-to-predict-hepatotoxicity
#15
Anselm Wong, Benjamin Cheung, Charlotte Nejad, Michael Gantier, Andis Graudins
CASE DETAILS: A 19-year-old girl presented to the emergency department following overdose of 10 g of paracetamol on a background history of cystic fibrosis. Paracetamol concentration was below the nomogram line, but was treated with acetylcysteine seven hours post-overdose given her symptomatology. Nineteen hours following her overdose she developed hepatotoxicity, despite early initiation of acetylcysteine. She was discharged well six days post-ingestion. On presentation, delta miRNA-122-miR483 was 20 times that of control patients, however, alanine aminotransferase was normal...
March 22, 2018: Clinical Toxicology
https://www.readbyqxmd.com/read/29564065/single-nucleotide-polymorphism-rs696-in-mir449a-binding-site-of-nfkbia-gene-is-correlated-with-risk-of-colorectal-cancer
#16
Miganoosh Simonian, Meysam Mosallayi, Maryam Miraghajani, Awat Feizi, Sharifeh Khosravi, Ahmad Reza Salehi, Deniz Mortazavi, Farideh Saberi, Rasoul Salehi
Aim: In present study we have elucidated the role of 2758 A>G (rs696), in the recognition site of miR449a in the 3' UTR of NFKB inhibitor alpha (NFKBIA) gene, in development of sporadic colorectal cancer. Background: Colorectal cancer (CRC) is rated as second cause of cancer death. Genetic determinants are considered as driving forces in development of sporadic CRC. Single nucleotide polymorphisms (SNPs), are attributed as the main genetic factor in cancers susceptibility...
2018: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/29562723/in-silico-integration-approach-to-identify-a-key-mirna-regulating-a-gene-network-in-aggressive-prostate-cancer
#17
Claudia Cava, Gloria Bertoli, Antonio Colaprico, Gianluca Bontempi, Giancarlo Mauri, Isabella Castiglioni
Like other cancer diseases, prostate cancer (PC) is caused by the accumulation of genetic alterations in the cells that drives malignant growth. These alterations are revealed by gene profiling and copy number alteration (CNA) analysis. Moreover, recent evidence suggests that also microRNAs have an important role in PC development. Despite efforts to profile PC, the alterations (gene, CNA, and miRNA) and biological processes that correlate with disease development and progression remain partially elusive. Many gene signatures proposed as diagnostic or prognostic tools in cancer poorly overlap...
March 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29559644/bone-related-circulating-micrornas-mir-29b-3p-mir-550a-3p-and-mir-324-3p-and-their-association-to-bone-microstructure-and-histomorphometry
#18
Xaver Feichtinger, Christian Muschitz, Patrick Heimel, Andreas Baierl, Astrid Fahrleitner-Pammer, Heinz Redl, Heinrich Resch, Elisabeth Geiger, Susanna Skalicky, Rainer Dormann, Fabian Plachel, Peter Pietschmann, Johannes Grillari, Matthias Hackl, Roland Kocijan
The assessment of bone quality and the prediction of fracture risk in idiopathic osteoporosis (IOP) are complex prospects as bone mineral density (BMD) and bone turnover markers (BTM) do not indicate fracture-risk. MicroRNAs (miRNAs) are promising new biomarkers for bone diseases, but the current understanding of the biological information contained in the variability of miRNAs is limited. Here, we investigated the association between serum-levels of 19 miRNA biomarkers of idiopathic osteoporosis to bone microstructure and bone histomorphometry based upon bone biopsies and µCT (9...
March 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29547527/the-oncogenic-relevance-of-mir-17-92-cluster-and-its-paralogous-mir-106b-25-and-mir-106a-363-clusters-in-brain-tumors
#19
REVIEW
Renata Gruszka, Magdalena Zakrzewska
The fundamental function of ribonucleic acids is to transfer genetic information from DNA to protein during translation process, however, this is not the only way connecting active RNA sequences with essential biological processes. Up until now, many RNA subclasses of different size, structure, and biological function were identified. Among them, there are non-coding single-stranded microRNAs (miRNAs). This subclass comprises RNAs of 19-25 nucleotides in length that modulate the activity of well-defined coding RNAs and play a crucial role in many physiological and pathological processes...
March 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29535815/serum-levels-of-mir-320-family-members-are-associated-with-clinical-parameters-and-diagnosis-in-prostate-cancer-patients
#20
Verena Lieb, Katrin Weigelt, Lena Scheinost, Kersten Fischer, Thomas Greither, Marios Marcou, Gerit Theil, Helmut Klocker, Hans-Juergen Holzhausen, Xin Lai, Julio Vera, Arif B Ekici, Wolfgang Horninger, Paolo Fornara, Bernd Wullich, Helge Taubert, Sven Wach
We studied the association of the serum levels of the microRNA family members miR-320a/-b/-c with clinico-pathological data to assess their applicability as diagnostic biomarker in prostate cancer (PCa) patients. The levels of miR-320a/-b/-c in 3 groups were evaluated by qRT-PCR (145 patients with PCa, 31 patients with benign prostatic hyperplasia (BPH) and 19 healthy controls). The levels of the three family members of miR-320 were directly correlated within each group ( P < 0.001), but they differed significantly among the three groups ( P < 0...
February 13, 2018: Oncotarget
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