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Joanna Miskiewicz, Marta Szachniuk
A small non-coding molecule of microRNA (19⁻24 nt) controls almost every biological process, including cellular and physiological, of various organisms’ lives. The amount of microRNA (miRNA) produced within an organism is highly correlated to the organism’s key processes, and determines whether the system works properly or not. A crucial factor in plant biogenesis of miRNA is the Dicer Like 1 (DCL1) enzyme. Its responsibility is to perform the cleavages in the miRNA maturation process...
June 6, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Qingbing Meng, Ming Dai, Xuejun Nie, Wensheng Zhang, Xingli Xu, Jian Li, Hongxin Mu, Xiaolan Liu, Ling Qin, Xiaoqi Zhu, Jun Yan, Minqian Zheng
This study was conducted to detect the expression of miR-19 and Pax6 (Paired box protein 6) in human osteosarcoma cells and the effects on biological characteristics of osteosarcoma cells. Quantitative real-time polymerase chain reaction was used to detect the expression of Pax6 and miR-19 in normal human osteoblasts (hFOB 1.19) and osteosarcoma cell lines (U2OS, Saos-2, and MG-63). Results showed that miR-19 was significantly upregulated in osteosarcoma cell lines compared with that in hFOB 1.19 cells, while the expression of Pax6 messenger RNA was significantly downregulated...
January 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Tian Wang, Ya-Ping Liu, Ting Wang, Bang-Qiang Xu, Bo Xu
In acute lung injury/acute respiratory distress syndrome (ALI/ARDS), pathogenesis is associated with the regulation of macrophage-generated oxidative stress, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)-derived reactive oxygen species(ROS) are key to regulating oxidative stress. In the present study, we found that miR-19 inhibited the expression of p47phox in macrophages, resulting in the alleviation of the lipopolysaccharides(LPS)-induced inflammatory response. In a mouse LPS-induced model of lung injury, miR-19-deficient murine lung tissue was more susceptible to inflammatory responses and exhibited a higher infiltration rate, a higher number of inflammatory cells in the lungs, a higher level of inflammatory cytokines in the Bronchoalveolar lavage fluid (BALF), and more severe pathological damage in lung tissues...
August 5, 2017: Biochemical and Biophysical Research Communications
Hidetoshi Eguchi, Yuichiro Doki, Masaki Mori
RNAs lacking protein-coding ability are called "noncoding RNAs" (ncRNAs). ncRNAs include microRNA (19-24 nucleotides in length) and long ncRNA (lncRNA). MicroRNA binds to the 3'-untranslated regions of target mRNAs and regulates target genes at a posttranscriptional level. In hepatocellular carcinoma (HCC), the roles of microRNAs in hepatocarcinogenesis and microRNAs that can determine the nature of HCC have been reported. Because microRNA is stable in the bloodstream or formalin-fixed specimens, it can be used as a tumor marker...
November 2015: Nihon Geka Gakkai Zasshi
Kai Xu, Ying-Chao Zhao
The underlying molecular pathogenesis in gastric cancer remains poorly unknown. The transcription factor myocyte enhancer factor 2D (MEF2D) participates in the initiation and development of many human cancers. However, its potential roles in gastric cancer have surprisingly not been studied. In present study, we first explored MEF2's expression in gastric cancer, finding that only MEF2D rather than MEF2A, 2B, or 2C was elevated in gastric cancer clinical specimens. Furthermore, immunohistochemical analysis on the tissue samples obtained from 260 patients with gastric cancer revealed that MEF2D expression was significantly associated with the clinical stage, vascular invasion, metastasis, and tumor size...
July 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Jing Li, Sheng Yang, Wen Yan, Jie Yang, Yu-Juan Qin, Xiao-Lin Lin, Rao-Ying Xie, Sheng-Chun Wang, Wen Jin, Fei Gao, Jun-Wen Shi, Wen-Tao Zhao, Jun-Shuang Jia, Hong-Fen Shen, Jie-Rong Ke, Bin Liu, Yi-Qiao Zhao, Wen-Hua Huang, Kai-Tai Yao, Dan-Juan Li, Dong Xiao
The miR-19 family (miR-19a and miR-19b-1) are key oncogenic components of the miR-17-92 cluster. Overexpression of miR-19 is strongly associated with cancer invasion and metastasis, and poor prognosis of cancer patients. However, the underlying mechanisms remain largely unknown. In the present study, we found that enforced expression of miR-19 including miR-19a and miR-19b-1 triggered epithelial-mesenchymal transition (EMT) of lung cancer cells A549 and HCC827 as shown by mesenchymal-like morphological conversion, downregulation of epithelial proteins (e...
September 2015: Laboratory Investigation; a Journal of Technical Methods and Pathology
Amy Lewis, Shameer Mehta, Luke N Hanna, Laura A Rogalski, Rosemary Jeffery, Anke Nijhuis, Tomoko Kumagai, Paolo Biancheri, Jake G Bundy, Cleo L Bishop, Roger Feakins, Antonio Di Sabatino, James C Lee, James O Lindsay, Andrew Silver
BACKGROUND: Development of fibrosis and subsequent stricture formation in Crohn's disease (CD) increases morbidity and rates of surgery and reduces patients' quality of life. There are currently no biomarkers of intestinal fibrosis that might allow earlier identification and better management of patients at increased risk of stricture formation. METHODS: MicroRNA profiling of serum from CD patients was used to identify microRNAs associated with stricture formation...
August 2015: Inflammatory Bowel Diseases
Oscar Peralta-Zaragoza, Faustino De-la-O-Gómez, Jessica Deas, Gloria Fernández-Tilapa, Geny Del Socorro Fierros-Zárate, Claudia Gómez-Cerón, Ana Burguete-García, Kirvis Torres-Poveda, Victor Hugo Bermúdez-Morales, Mauricio Rodríguez-Dorantes, Carlos Pérez-Plasencia, Vicente Madrid-Marina
RNA interference is a natural mechanism to silence post-transcriptional gene expression in eukaryotic cells in which microRNAs act to cleave or halt the translation of target mRNAs at specific target sequences. Mature microRNAs, 19-25 nucleotides in length, mediate their effect at the mRNA level by inhibiting translation, or inducing cleavage of the mRNA target. This process is directed by the degree of complementary nucleotides between the microRNAs and the target mRNA; perfect complementary base pairing induces cleavage of mRNA, whereas several mismatches lead to translational arrest...
2015: Methods in Molecular Biology
D Hühn, A N Kousholt, C S Sørensen, A A Sartori
MicroRNA-19 (miR-19) was recently identified as the key oncogenic component of the polycistronic miR-17∼92 cluster, also known as oncomiR-1, which is frequently upregulated or amplified in multiple tumor types. However, the gene targets and the pathways underlying the tumor-promoting activity of miR-19 still remain largely elusive. CtIP/RBBP8 promotes DNA-end resection, a critical step in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR), and is considered to function as a tumor suppressor...
July 23, 2015: Oncogene
Michael P Gantier
No abstract text is available yet for this article.
August 2013: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
Claudio Minoia, Elena Sturchio, Benedetta Porro, Barbara Ficociello, Alberto Zambelli, Marcello Imbriani
Malignant mesothelioma is an aggressive cancer refractory to current therapies, the incidence of which is expected to rise in the next decades. Exposure to asbestos is a well known risk factor, as InternationalAgency for Research and Cancer (IARC) classified this compound as group I (carcinogenic to humans). The lack of tumor biomarkers for diagnosis and medical survey plays a fundamental role for the development of a universally accepted therapeutic approach. In this review we evaluated the mechanism of asbestos carcinogenesis by analyzing activated oncogenes, genetic predisposition, and SV40 infection as cofactors...
October 2011: Giornale Italiano di Medicina del Lavoro Ed Ergonomia
Geert C van Almen, Wouter Verhesen, Rick E W van Leeuwen, Mathijs van de Vrie, Casper Eurlings, Mark W M Schellings, Melissa Swinnen, Jack P M Cleutjens, Marc A M J van Zandvoort, Stephane Heymans, Blanche Schroen
To understand the process of cardiac aging, it is of crucial importance to gain insight into the age-related changes in gene expression in the senescent failing heart. Age-related cardiac remodeling is known to be accompanied by changes in extracellular matrix (ECM) gene and protein levels. Small noncoding microRNAs regulate gene expression in cardiac development and disease and have been implicated in the aging process and in the regulation of ECM proteins. However, their role in age-related cardiac remodeling and heart failure is unknown...
October 2011: Aging Cell
Xiaoxi Zhang, Haijun Yu, Jessica R Lou, Jie Zheng, Hua Zhu, Narcis-Ioan Popescu, Florea Lupu, Stuart E Lind, Wei-Qun Ding
Tissue factor has been recognized as a regulator of tumor angiogenesis and metastasis. The tissue factor gene is selectively expressed in highly invasive breast cancer cells, and the mechanisms regulating tissue factor expression in these cells remain unclear. This study demonstrates that microRNA-19 (miR-19) regulates tissue factor expression in breast cancer cells, providing a molecular basis for the selective expression of the tissue factor gene. Tissue factor protein was barely detectable in MCF-7, T47D, and ZR-75-1 cells (less invasive breast lines) but was expressed at a significantly higher level in MDA-MB-231 and BT-20 cells (invasive breast lines) as assayed by Western blot...
January 14, 2011: Journal of Biological Chemistry
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