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https://www.readbyqxmd.com/read/27846359/-o-arm-navigation-in-spinal-surgery-for-complex-cases
#1
D Pescador, D Rendón, J Blanco, R González, J M Martín, C Cano-Gala, D Pescador
The intraoperative computer-assisted navigation technique has evolved and changed the way we approach certain complex cases in the vertebral pathologies. The main advantages of this technique are that it increases the accuracy of the procedure, reduces its morbidity and invasiveness, diminishes the radiation dose and improves surgical protocols. In addition to the transpedicular fixation, the navigation system is useful in a high variety of pathologies such as spinal decompression or tumor resection. In the present study, we show several cases where navigation is used intraoperatively in different pathologies and their clinical outcomes...
March 2016: Acta Ortopédica Mexicana
https://www.readbyqxmd.com/read/27686967/rapamycin-negatively-impacts-insulin-signaling-glucose-uptake-and-uncoupling-protein-1-in-brown-adipocytes
#2
Ester García-Casarrubios, Carlos de Moura, Ana I Arroba, Nuria Pescador, María Calderon-Dominguez, Laura Garcia, Laura Herrero, Dolors Serra, Susana Cadenas, Flavio Reis, Eugenia Carvalho, Maria Jesus Obregon, Ángela M Valverde
New onset diabetes after transplantation (NODAT) is a metabolic disorder that affects 40% of patients on immunosuppressive agent (IA) treatment, such as rapamycin (also known as sirolimus). IAs negatively modulate insulin action in peripheral tissues including skeletal muscle, liver and white fat. However, the effects of IAs on insulin sensitivity and thermogenesis in brown adipose tissue (BAT) have not been investigated. We have analyzed the impact of rapamycin on insulin signaling, thermogenic gene-expression and mitochondrial respiration in BAT...
September 26, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27642606/networks-models-of-actin-dynamics-during-spermatozoa-postejaculatory-life-a-comparison-among-human-made-and-text-mining-based-models
#3
Nicola Bernabò, Alessandra Ordinelli, Marina Ramal Sanchez, Mauro Mattioli, Barbara Barboni
Here we realized a networks-based model representing the process of actin remodelling that occurs during the acquisition of fertilizing ability of human spermatozoa (HumanMade_ActinSpermNetwork, HM_ASN). Then, we compared it with the networks provided by two different text mining tools: Agilent Literature Search (ALS) and PESCADOR. As a reference, we used the data from the online repository Kyoto Encyclopaedia of Genes and Genomes (KEGG), referred to the actin dynamics in a more general biological context. We found that HM_ALS and the networks from KEGG data shared the same scale-free topology following the Barabasi-Albert model, thus suggesting that the information is spread within the network quickly and efficiently...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27637760/comparative-analysis-of-the-immunomodulatory-capacities-of-human-bone-marrow-and-adipose-tissue-derived-mesenchymal-stromal-cells-from-the-same-donor
#4
Jaris Valencia, Belén Blanco, Rosa Yáñez, Miriam Vázquez, Carmen Herrero Sánchez, María Fernández-García, Concepción Rodríguez Serrano, David Pescador, Juan F Blanco, Miriam Hernando-Rodríguez, Fermín Sánchez-Guijo, María Luisa Lamana, José Carlos Segovia, Ángeles Vicente, Consuelo Del Cañizo, Agustín G Zapata
BACKGROUND AIMS: The immunomodulatory properties of mesenchymal stromal cells (MSCs), together with their tissue regenerative potential, make them interesting candidates for clinical application. METHODS: In the current study, we analyzed the in vitro immunomodulatory effects of MSCs derived from bone marrow (BM-MSCs) and from adipose tissue (AT-MSCs) obtained from the same donor on both innate and acquired immunity cells. BM-MSCs and AT-MSCs were expanded to fourth or fifth passage and co-cultured with T cells, monocytes or natural killer (NK) cells isolated from human peripheral blood and stimulated in vitro...
October 2016: Cytotherapy
https://www.readbyqxmd.com/read/27462351/filaggrin-gene-mutations-and-new-snps-in-asthmatic-patients-a-cross-sectional-study-in-a-spanish-population
#5
José Luis Cubero, María Isidoro-García, Nieves Segura, David Benito Pescador, Catalina Sanz, Félix Lorente, Ignacio Dávila, Carlos Colás
BACKGROUND: Several null-mutations in the FLG gene that produce a decrease or absence of filaggrin in the skin and predispose to atopic dermatitis and ichthyosis vulgaris have been described. The relationship with asthma is less clear and may be due to the influence of atopy in patients with associated asthma. METHODS: Four hundred individuals were included, 300 patients diagnosed with asthma divided into two groups according to their phenotype (allergic and non-allergic asthma) and 100 strictly characterized controls...
2016: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/27394219/species-diversity-of-fiddler-crabs-genus-uca-leach-1814-crustacea-ocypodidae-from-taiwan-and-adjacent-islands-with-notes-on-the-japanese-species
#6
Hsi-Te Shih, Jung-Hsiang Lee, Ping-Ho Ho, Hung-Chang Liu, Chia-Hsiang Wang, Hiroshi Suzuki, Shao-Jyun Teng
The fiddler crabs, genus Uca Leach, 1814 (Decapoda, Ocypodidae) of Taiwan, including the offshore islands of Penghu (Pescadores), Kinmen (Quemoy), Matsu (Matzu), and Dongsha (Pratas), are revised, with the recognition of five subgenera and 15 species, viz. Uca (Austruca) Bott, 1973: U. lactea (De Haan, 1835), U. perplexa (H. Milne Edwards, 1837), U. triangularis (A. Milne-Edwards, 1873); U. (Gelasimus) Latreille, 1817: U. borealis Crane, 1975, U. jocelynae Shih, Naruse & Ng, 2010, U. tetragonon (Herbst, 1790), U...
2016: Zootaxa
https://www.readbyqxmd.com/read/27334828/hemostatic-assessment-of-dogs-associated-with-hepatic-parasite-load-of-leishmania-infantum-chagasi
#7
Mariana de Medeiros Torres, Arleana do Bom Parto Ferreira de Almeida, Daphine Ariadne Jesus de Paula, Adriane Jorge Mendonça, Luciano Nakazato, Caroline Argenta Pescador, Valéria Régia Franco Sousa
Leishmania infantum chagasi liver parasite load was compared to hemostatic abnormalities, as well as to clinical, laboratorial, and histopathological findings in dogs with visceral leishmaniasis. The liver parasite load of 30 dogs L. infantum chagasi naturally-infected was evaluated by quantitative real- time PCR and the results were compared with serum biochemistry and primary and secondary hemostasis findings. Moreover, hepatic histological lesions were described in these dogs. Prolonged bleeding time, prothrombin time (PT), and activated partial thromboplastin time (APTT), were observed in the group with visceral leishmaniasis...
June 14, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27304522/hemostatic-assessment-of-dogs-associated-with-hepatic-parasite-load-of-leishmania-infantum-chagasi
#8
Mariana de Medeiros Torres, Arleana do Bom Parto Ferreira de Almeida, Daphine Ariadne Jesus de Paula, Adriane Jorge Mendonça, Luciano Nakazato, Caroline Argenta Pescador, Valéria Régia Franco Sousa
Leishmania infantum chagasi liver parasite load was compared to hemostatic abnormalities, as well as to clinical, laboratorial, and histopathological findings in dogs with visceral leishmaniasis. The liver parasite load of 30 dogs L. infantum chagasi naturally-infected was evaluated by quantitative real- time PCR and the results were compared with serum biochemistry and primary and secondary hemostasis findings. Moreover, hepatic histological lesions were described in these dogs. Prolonged bleeding time, prothrombin time (PT), and activated partial thromboplastin time (APTT), were observed in the group with visceral leishmaniasis...
June 14, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27244389/ultraviolet-femtosecond-kerr-gated-wide-field-fluorescence-microscopy
#9
Jolie C Blake, Jesus Nieto-Pescador, Zhengxin Li, Lars Gundlach
A Kerr-gated microscope capable of imaging ultraviolet luminescence with femtosecond time resolution has been developed. The system allows the spatial, spectral, and temporal measurement of UV-emitting samples. The instrumentation was optimized for emission collection in the UV, resulting in sub 90 fs time resolution of gated signals. ZnO nanowires were used to demonstrate the performance of the instrument. The evolution of the emission from a single nanowire was tracked via ultrafast transient spectroscopy and through sequential imaging...
June 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/26952144/bcmimp1-a-botrytis-cinerea-gene-transiently-expressed-in-planta-encodes-a-mitochondrial-protein
#10
David Benito-Pescador, Daniela Santander, M Arranz, José M Díaz-Mínguez, Arturo P Eslava, Jan A L van Kan, Ernesto P Benito
Botrytis cinerea is a widespread necrotrophic fungus which infects more than 200 plant species. In an attempt to characterize the physiological status of the fungus in planta and to identify genetic factors contributing to its ability to infect the host cells, a differential gene expression analysis during the interaction B. cinerea-tomato was carried out. Gene Bcmimp1 codes for a mRNA detected by differential display in the course of this analysis. During the interaction with the host, it shows a transient expression pattern with maximal expression levels during the colonization and maceration of the infected tissues...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26941761/summer-freezing-resistance-a-critical-filter-for-plant-community-assemblies-in-mediterranean-high-mountains
#11
David S Pescador, Ángela Sierra-Almeida, Pablo J Torres, Adrián Escudero
Assessing freezing community response and whether freezing resistance is related to other functional traits is essential for understanding alpine community assemblages, particularly in Mediterranean environments where plants are exposed to freezing temperatures and summer droughts. Thus, we characterized the leaf freezing resistance of 42 plant species in 38 plots at Sierra de Guadarrama (Spain) by measuring their ice nucleation temperature, freezing point (FP), and low-temperature damage (LT50), as well as determining their freezing resistance mechanisms (i...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/26909618/marine-protected-area-and-the-spatial-distribution-of-the-gill-net-fishery-in-copacabana-rio-de-janeiro-rj-brazil
#12
R B Amorim, C Monteiro-Neto
This study characterizes the gill net fishery at Colônia de Pescadores Z13 (CPZ13), in Copacabana, Rio de Janeiro, and its relationship with the marine protected area 'Monumento Natural do Arquipélago das Ilhas Cagarras - MoNa Cagarras', describing the fleet and fishing gears, identifying fishing spots, species and their associations by gillnet type. From June 2012 to May 2013, every Tuesday to Sunday, gill net landings were monitored and fishers interviewed regarding their catch. Small boats (dory whaleboats) are used to set three types of gillnets: "Corvineira" (target species - whitemouth croaker), "linguadeira" (target species - flounders) and "rede-alta" (target species - bluefish)...
February 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/26899503/evaluation-of-combined-use-of-the-maldi-tof-and-genomera-mrsa-sa-assay-for-the-direct-detection-of-methicillin-resistance-in-staphylococcus-aureus-from-positive-blood-culture-bottles
#13
Paula Pescador, María Pilar Romero-Gómez, Rosa Gómez Gil, Jesús Mingorance
No abstract text is available yet for this article.
February 15, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/26894995/efficient-z-scheme-charge-separation-in-novel-vertically-aligned-zno-cdsse-nanotrees
#14
Zhengxin Li, Jesus Nieto-Pescador, Alexander J Carson, Jolie C Blake, Lars Gundlach
A new tree-like ZnO/CdSSe nanocomposite with CdSSe branches grown on ZnO nanowires prepared via a two-step chemical vapor deposition is presented. The nanotrees (NTs) are vertically aligned on a substrate. The CdSSe branches result in strong visible light absorption and form a type-II heterojunction with the ZnO stem that facilitates efficient electron transfer. A combination of photoluminescence spectroscopy and lifetime measurements indicates that the NTs are promising materials for applications that benefit from a Z-scheme charge transfer mechanism...
April 1, 2016: Nanotechnology
https://www.readbyqxmd.com/read/26840575/marine-protected-area-and-the-spatial-distribution-of-the-gill-net-fishery-in-copacabana-rio-de-janeiro-rj-brazil
#15
R B Amorim, C Monteiro-Neto
This study characterizes the gill net fishery at Colônia de Pescadores Z13 (CPZ13), in Copacabana, Rio de Janeiro, and its relationship with the marine protected area 'Monumento Natural do Arquipélago das Ilhas Cagarras - MoNa Cagarras', describing the fleet and fishing gears, identifying fishing spots, species and their associations by gillnet type. From June 2012 to May 2013, every Tuesday to Sunday, gill net landings were monitored and fishers interviewed regarding their catch. Small boats (dory whaleboats) are used to set three types of gillnets: "Corvineira" (target species - whitemouth croaker), "linguadeira" (target species - flounders) and "rede-alta" (target species - bluefish)...
January 22, 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/26820565/individual-species-affect-plant-traits-structure-in-their-surroundings-evidence-of-functional-mechanisms-of-assembly
#16
Julia Chacón-Labella, Marcelino de la Cruz, David S Pescador, Adrián Escudero
Evaluating community assembly through the use of functional traits is a promising tool for testing predictions arising from Niche and Coexistence theories. Although interactions among neighboring species and their inter-specific differences are known drivers of coexistence with a strong spatial signal, assessing the role of individual species on the functional structure of the community at different spatial scales remains a challenge. Here, we ask whether individual species exert a measurable effect on the spatial organization of different functional traits in local assemblages...
April 2016: Oecologia
https://www.readbyqxmd.com/read/26620852/impact-of-size-secondary-structure-and-counterions-on-the-binding-of-small-ribonucleic-acids-to-layered-double-hydroxide-nanoparticles
#17
Blanca V Rodriguez, Jorge Pescador, Nicole Pollok, Gary W Beall, Corina Maeder, L Kevin Lewis
Use of ribonucleic acid (RNA) interference to regulate protein expression has become an important research topic and gene therapy tool, and therefore, finding suitable vehicles for delivery of small RNAs into cells is of crucial importance. Layered double metal hydroxides such as hydrotalcite (HT) have shown great promise as nonviral vectors for transport of deoxyribose nucleic acid (DNA), proteins, and drugs into cells, but the adsorption of RNAs to these materials has been little explored. In this study, the binding of small RNAs with different lengths and levels of secondary structure to HT nanoparticles has been analyzed and compared to results obtained with small DNAs in concurrent experiments...
December 30, 2015: Biointerphases
https://www.readbyqxmd.com/read/26452680/finite-element-analysis-and-cadaveric-cinematic-analysis-of-fixation-options-for-anteriorly-implanted-trabecular-metal-interbody-cages
#18
Pedro Berjano, Juan Francisco Blanco, Diego Rendon, Jorge Hugo Villafañe, David Pescador, Carlos Manuel Atienza
PURPOSE: To assess, with finite element analysis and an in vitro biomechanical study in cadaver, whether the implementation of an anterior interbody cage made of hedrocel with nitinol shape memory staples in compression increases the stiffness of the stand-alone interbody cage and to compare these constructs' stiffness to other constructs common in clinical practice. METHODS: A biomechanical study with a finite element analysis and cadaveric testing assessed the stiffness of different fixation modes for the L4-L5 functional spinal unit: intact spine, destabilized spine with discectomy, posterior pedicle-screw fixation, anterior stand-alone interbody cage, anterior interbody cage with bilateral pedicle screws and anterior interbody cage with two shape memory staples in compression...
November 2015: European Spine Journal
https://www.readbyqxmd.com/read/26415862/genetic-diversity-of-brazilian-bovine-pestiviruses-detected-between-1995-and-2014
#19
S Silveira, M N Weber, A C S Mósena, M S da Silva, A F Streck, C A Pescador, E F Flores, R Weiblen, D Driemeier, J F Ridpath, C W Canal
Pestivirus infections in ruminants result in significant economic losses worldwide. The aetiological agents are three species from the genus Pestivirus, family Flaviviridae, including bovine viral diarrhoea virus type 1 (BVDV-1), BVDV-2, border disease virus (BDV), and an atypical pestivirus named HoBi-like pestivirus. In this study, eighty-nine pestivirus isolates that were collected in Brazil between 1995 and 2014 and that originated from either cattle, fetal bovine serum (FBS) or as cell culture contaminants were genotyped based on a comparison of gene sequences from their 5' untranslated regions (5'UTR), N-terminal autoprotease (N(pro) ) and envelope glycoprotein 2 (E2)...
September 28, 2015: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/26278600/photoinduced-ultrafast-heterogeneous-electron-transfer-at-molecule-semiconductor-interfaces
#20
Jesus Nieto-Pescador, Baxter Abraham, Lars Gundlach
This Perspective discusses recent developments in ultrafast electron transfer dynamics at interfaces between organic and inorganic materials. Heterogeneous electron transfer (HET) is a key process in important fields like catalysis and solar energy conversion. Furthermore, the solid state nature of the systems gives control over relevant parameters and allows for investigating excited state dynamics and electron transfer processes in unprecedented detail. Progress in synthesis, sample preparation, and instrumentation makes it possible to provide experimental proof of recent prediction from theory concerning the adiabaticity of the reaction and the influence of coherence...
October 16, 2014: Journal of Physical Chemistry Letters
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