keyword
https://read.qxmd.com/read/38493496/chromatin-modifier-ep400-regulates-oocyte-quality-and-zygotic-genome-activation-in-mice
#1
JOURNAL ARTICLE
Qing Tian, Ying Yin, Yu Tian, Yufan Wang, Yong-Feng Wang, Rikiro Fukunaga, Toshihiro Fujii, Ai-Hua Liao, Lei Li, Wei Zhang, Ximiao He, Wenpei Xiang, Li-Quan Zhou
Epigenetic modifiers that accumulate in oocytes, play a crucial role in steering the developmental program of cleavage embryos and initiating life. However, the identification of key maternal epigenetic regulators remains elusive. In the findings, the essential role of maternal Ep400, a chaperone for H3.3, in oocyte quality and early embryo development in mice is highlighted. Depletion of Ep400 in oocytes resulted in a decline in oocyte quality and abnormalities in fertilization. Preimplantation embryos lacking maternal Ep400 exhibited reduced major zygotic genome activation (ZGA) and experienced developmental arrest at the 2-to-4-cell stage...
March 17, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38493245/metabolomic-profiles-of-ovariectomized-mice-and-their-associations-with-body-composition-and-frailty-related-parameters-in-postmenopausal-women
#2
JOURNAL ARTICLE
S J Kim, Y Jo, S J Park, E Ji, J Y Lee, E Choi, J-Y Baek, I Y Jang, H-W Jung, K Kim, D Ryu, H J Yoo, B-J Kim
BACKGROUND: Menopause, a dramatical estrogen-deficient condition, is considered the most significant milestone in women's health. PURPOSE: To investigate the metabolite changes attributed to estrogen deficiency using random forest (RF)-based machine learning (ML) modeling strategy in ovariectomized (OVX) mice as well as determine the clinical relevance of selected metabolites in older women. METHODS AND RESULTS: Untargeted and targeted metabolomic analyses revealed that metabolites related to TCA cycle, sphingolipids, phospholipids, fatty acids, and amino acids, were significantly changed in the plasma and/or muscle of OVX mice...
March 17, 2024: Journal of Endocrinological Investigation
https://read.qxmd.com/read/38492486/-deciphering-the-enigmatic-role-of-gamma-aminobutyric-acid-gaba-in-plants-synthesis-transport-regulation-signaling-and-biological-roles-in-interaction-with-growth-regulators-and-abiotic-stresses
#3
REVIEW
Saif Ahmad, Qazi Fariduddin
Gamma-aminobutyric acid (GABA) is an amino acid with a four-carbon structure, widely distributed in various organisms. It exists as a zwitterion, possessing both positive and negative charges, enabling it to interact with other molecules and participate in numerous physiological processes. GABA is widely distributed in various plant cell compartments such as cytoplasm mitochondria, vacuoles, peroxisomes, and plastids. GABA is primarily synthesized from glutamate using glutamate decarboxylase and participates in the GABA shunt within mitochondria, regulating carbon and nitrogen metabolism in plants The transport of GABA is regulated by several intracellular and intercellular transporters such as aluminium-activated malate transporters (ALMTs), GABA transporters (GATs), bidirectional amino acid transporters (BATs), and cationic amino acid transporters (CATs)...
March 8, 2024: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/38492251/parallel-functional-reduction-in-the-mitochondria-of-apicomplexan-parasites
#4
JOURNAL ARTICLE
Patrick J Keeling, Mahara Mtawali, Morelia Trznadel, Samuel J Livingston, Kevin C Wakeman
Extreme functional reduction of mitochondria has taken place in parallel in many distantly related lineages of eukaryotes, leading to a number of recurring metabolic states with variously lost electron transport chain (ETC) complexes, loss of the tricarboxylic acid (TCA) cycle, and/or loss of the mitochondrial genome. The resulting mitochondria-related organelles (MROs) are generally structurally reduced and in the most extreme cases barely recognizable features of the cell with no role in energy metabolism whatsoever (e...
March 9, 2024: European Journal of Protistology
https://read.qxmd.com/read/38491007/a-citric-acid-cycle-deficient-escherichia-coli-as-an-efficient-chassis-for-aerobic-fermentations
#5
JOURNAL ARTICLE
Hang Zhou, Yiwen Zhang, Christopher P Long, Xuesen Xia, Yanfen Xue, Yanhe Ma, Maciek R Antoniewicz, Yong Tao, Baixue Lin
Tricarboxylic acid cycle (TCA cycle) plays an important role for aerobic growth of heterotrophic bacteria. Theoretically, eliminating TCA cycle would decrease carbon dissipation and facilitate chemicals biosynthesis. Here, we construct an E. coli strain without a functional TCA cycle that can serve as a versatile chassis for chemicals biosynthesis. We first use adaptive laboratory evolution to recover aerobic growth in minimal medium of TCA cycle-deficient E. coli. Inactivation of succinate dehydrogenase is a key event in the evolutionary trajectory...
March 15, 2024: Nature Communications
https://read.qxmd.com/read/38490069/atf3-cbs-signaling-axis-coordinates-ferroptosis-and-tumorigenesis-in-colorectal-cancer
#6
JOURNAL ARTICLE
Junjia Liu, Xinyi Lu, Siyu Zeng, Rong Fu, Xindong Wang, Lingtao Luo, Ting Huang, Xusheng Deng, Hualei Zheng, Shaoqian Ma, Dan Ning, Lili Zong, Shu-Hai Lin, Yongyou Zhang
The induction of ferroptosis is promising for cancer therapy. However, the mechanisms enabling cancer cells to evade ferroptosis, particularly in low-cystine environments, remain elusive. Our study delves into the intricate regulatory mechanisms of Activating transcription factor 3 (ATF3) on Cystathionine β-synthase (CBS) under cystine deprivation stress, conferring resistance to ferroptosis in colorectal cancer (CRC) cells. Additionally, our findings establish a positively correlation between this signaling axis and CRC progression, suggesting its potential as a therapeutic target...
March 8, 2024: Redox Biology
https://read.qxmd.com/read/38487538/control-of-immune-cell-signaling-by-the-immuno-metabolite-itaconate
#7
REVIEW
Roland Lang, Md Nur A Alam Siddique
Immune cell activation triggers signaling cascades leading to transcriptional reprogramming, but also strongly impacts on the cell's metabolic activity to provide energy and biomolecules for inflammatory and proliferative responses. Macrophages activated by microbial pathogen-associated molecular patterns and cytokines upregulate expression of the enzyme ACOD1 that generates the immune-metabolite itaconate by decarboxylation of the TCA cycle metabolite cis-aconitate. Itaconate has anti-microbial as well as immunomodulatory activities, which makes it attractive as endogenous effector metabolite fighting infection and restraining inflammation...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38486750/copper-metabolism-and-cuproptosis-in-human-malignancies-unraveling-the-complex-interplay-for-therapeutic-insights
#8
REVIEW
K M Abdullah, Jyoti B Kaushal, Simran Takkar, Gunjan Sharma, Zahraa W Alsafwani, Ramesh Pothuraju, Surinder Kumar Batra, Jawed Akhtar Siddiqui
Copper, a vital trace element, orchestrates diverse cellular processes ranging from energy production to antioxidant defense and angiogenesis. Copper metabolism and cuproptosis are closely linked in the context of human diseases, with a particular focus on cancer. Cuproptosis refers to a specific type of copper-mediated cell death or copper toxicity triggered by disruptions in copper metabolism within the cells. This phenomenon encompasses a spectrum of mechanisms, such as oxidative stress, mitochondrial dysfunction, endoplasmic reticulum stress, and perturbations in metal ion equilibrium...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38484742/cross-validation-of-metabolic-phenotypes-in-sars-cov-2-infected-subpopulations-using-targeted-liquid-chromatography-mass-spectrometry-lc-ms
#9
JOURNAL ARTICLE
Luke Whiley, Nathan G Lawler, Annie Xu Zeng, Alex Lee, Sung-Tong Chin, Maider Bizkarguenaga, Chiara Bruzzone, Nieves Embade, Julien Wist, Elaine Holmes, Oscar Millet, Jeremy K Nicholson, Nicola Gray
To ensure biological validity in metabolic phenotyping, findings must be replicated in independent sample sets. Targeted workflows have long been heralded as ideal platforms for such validation due to their robust quantitative capability. We evaluated the capability of liquid chromatography-mass spectrometry (LC-MS) assays targeting organic acids and bile acids to validate metabolic phenotypes of SARS-CoV-2 infection. Two independent sample sets were collected: (1) Australia: plasma, SARS-CoV-2 positive ( n = 20), noninfected healthy controls ( n = 22) and COVID-19 disease-like symptoms but negative for SARS-CoV-2 infection ( n = 22)...
March 14, 2024: Journal of Proteome Research
https://read.qxmd.com/read/38483541/ogdh-and-bcl-xl-loss-causes-synthetic-lethality-in-glioblastoma
#10
JOURNAL ARTICLE
Trang Tt Nguyen, Consuelo Torrini, Enyuan Shang, Chang Shu, Jeong-Yeon Mun, Qiuqiang Gao, Nelson Humala, Hasan O Akman, Guoan Zhang, Mike-Andrew Westhoff, Georg Karpel-Massler, Jeffrey N Bruce, Peter Canoll, Markus D Siegelin
Glioblastoma (GBM) remains an incurable disease, requiring more effective therapies. Through interrogation of publicly available CRISPR and RNAi library screens, we identified the alpha-ketoglutarate dehydrogenase (OGDH) gene, which encodes for an enzyme that is part of the tricarboxylic acid cycle (TCA cycle) as essential for GBM growth. Moreover, by combining a transcriptome and metabolite screening analyses we discovered that loss of function of OGDH by the clinically validated drug compound, CPI-613, was synthetically lethal with Bcl-xL inhibition (genetically and through the clinically validated BH3-mimetic, ABT263) in patient-derived xenograft as well neurosphere GBM cultures...
March 14, 2024: JCI Insight
https://read.qxmd.com/read/38483038/metabolic-rewiring-enables-ammonium-assimilation-via-a-non-canonical-fumarate-based-pathway
#11
JOURNAL ARTICLE
Mohammad Saba Yousef Mardoukhi, Johanna Rapp, Iker Irisarri, Katrin Gunka, Hannes Link, Jan Marienhagen, Jan de Vries, Jörg Stülke, Fabian M Commichau
Glutamate serves as the major cellular amino group donor. In Bacillus subtilis, glutamate is synthesized by the combined action of the glutamine synthetase and the glutamate synthase (GOGAT). The glutamate dehydrogenases are devoted to glutamate degradation in vivo. To keep the cellular glutamate concentration high, the genes and the encoded enzymes involved in glutamate biosynthesis and degradation need to be tightly regulated depending on the available carbon and nitrogen sources. Serendipitously, we found that the inactivation of the ansR and citG genes encoding the repressor of the ansAB genes and the fumarase, respectively, enables the GOGAT-deficient B...
March 2024: Microbial Biotechnology
https://read.qxmd.com/read/38481385/sirt5-mediated-lysine-desuccinylation-regulates-oxidative-stress-adaptation-in-magnaporthe-oryzae-during-host-intracellular-infection
#12
JOURNAL ARTICLE
Zhiyong Ren, Xiang Dong, Lun Guan, Lei Yang, Caiyun Liu, Xuan Cai, Hong Hu, Ziwei Lv, Hao Liu, Lu Zheng, Junbin Huang, Richard A Wilson, Xiao-Lin Chen
Plant pathogenic fungi elaborate numerous detoxification strategies to suppress host reactive oxygen species (ROS), but their coordination is not well-understood. Here, we show that Sirt5-mediated protein desuccinylation in Magnaporthe oryzae is central to host ROS detoxification. SIRT5 encodes a desuccinylase important for virulence via adaptation to host oxidative stress. Quantitative proteomics analysis identified a large number of succinylated proteins targeted by Sirt5, most of which were mitochondrial proteins involved in oxidative phosphorylation, TCA cycle, and fatty acid oxidation...
March 14, 2024: New Phytologist
https://read.qxmd.com/read/38479551/metabolic-alterations-and-mitochondrial-dysfunction-in-human-airway-beas-2b-cells-exposed-to-vanadium-pentoxide
#13
JOURNAL ARTICLE
Xiaojia He, Matthew Ryan Smith, Zachery R Jarrell, ViLinh Thi Ly, Yongliang Liang, Choon-Myung Lee, Michael Orr, Young-Mi Go, Dean P Jones
Vanadium pentoxide (V+5 ) is a hazardous material that has drawn considerable attention due to its wide use in industrial sectors and increased release into environment from human activities. It poses potential adverse effects on animals and human health, with pronounced impact on lung physiology and functions. In this study, we investigated the metabolic response of human bronchial epithelial BEAS-2B cells to low-level V+5 exposure (0.01, 0.1, and 1 ppm) using liquid chromatography-high resolution mass spectrometry (LC-HRMS)...
March 11, 2024: Toxicology
https://read.qxmd.com/read/38478874/environmentally-relevant-concentrations-of-tetrabromobisphenol-a-exposure-impends-neurovascular-formation-through-perturbing-mitochondrial-metabolism-in-zebrafish-embryos-and-human-primary-endothelial-cells
#14
JOURNAL ARTICLE
Xiangyu Zeng, Shengtao Ma, Yijun Luo, Yangjian Zhang, Qi Wang, Zhuyi Zhang, Weijian Ke, Ya Ma, Haichen Hu, Thomas Hartung, Yanhong Wei, Xiali Zhong
Tetrabromobisphenol A (TBBPA), the most extensively utilized brominated flame retardant, has raised growing concerns regarding its environmental and health risks. Neurovascular formation is essential for metabolically supporting neuronal networks. However, previous studies primarily concerned the neuronal injuries of TBBPA, its impact on the neurovascularture, and molecular mechanism, which are yet to be elucidated. In this study, 5, 30, 100, 300 μg/L of TBBPA were administered to Tg (fli1a: eGFP) zebrafish larvae at 2-72 h postfertilization (hpf)...
March 13, 2024: Environmental Science & Technology
https://read.qxmd.com/read/38477776/hypoxia-rewires-glucose-and-glutamine-metabolism-in-different-sources-of-skeletal-stem-and-progenitor-cells-similarly-except-for-pyruvate
#15
JOURNAL ARTICLE
Shauni Loopmans, Guillaume Tournaire, Ingrid Stockmans, Steve Stegen, Geert Carmeliet
Skeletal stem and progenitor cells (SSPCs) are crucial for bone development, homeostasis, and repair. SSPCs are considered to reside in a rather hypoxic niche in the bone, but distinct SSPC niches have been described in different skeletal regions, and they likely differ in oxygen and nutrient availability. Currently it remains unknown whether the different SSPC sources have a comparable metabolic profile and respond in a similar manner to hypoxia. In this study, we show that cell proliferation of all SSPCs was increased in hypoxia, suggesting that SSPCs can indeed function in a hypoxic niche in vivo...
January 11, 2024: Journal of Bone and Mineral Research
https://read.qxmd.com/read/38475505/aging-induced-reduction-in-safflower-seed-germination-via-impaired-energy-metabolism-and-genetic-integrity-is-partially-restored-by-sucrose-and-da-6-treatment
#16
JOURNAL ARTICLE
Tang Lv, Juan Li, Lanyu Zhou, Tao Zhou, Hugh W Pritchard, Chaoxiang Ren, Jiang Chen, Jie Yan, Jin Pei
Seed storage underpins global agriculture and the seed trade and revealing the mechanisms of seed aging is essential for enhancing seed longevity management. Safflower is a multipurpose oil crop, rich in unsaturated fatty acids that are at high risk of peroxidation as a contributory factor to seed aging. However, the molecular mechanisms responsible for safflower seed viability loss are not yet elucidated. We used controlled deterioration (CDT) conditions of 60% relative humidity and 50 °C to reduce germination in freshly harvested safflower seeds and analyzed aged seeds using biochemical and molecular techniques...
February 27, 2024: Plants (Basel, Switzerland)
https://read.qxmd.com/read/38473844/nicotinamide-mononucleotide-nmn-works-in-type-2-diabetes-through-unexpected-effects-in-adipose-tissue-not-by-mitochondrial-biogenesis
#17
JOURNAL ARTICLE
Roua Gabriela Popescu, Anca Dinischiotu, Teodoru Soare, Ene Vlase, George Cătălin Marinescu
Nicotinamide mononucleotide (NMN) has emerged as a promising therapeutic intervention for age-related disorders, including type 2 diabetes. In this study, we confirmed the previously observed effects of NMN treatment on glucose uptake and investigated its underlying mechanisms in various tissues and cell lines. Through the most comprehensive proteomic analysis to date, we discovered a series of novel organ-specific effects responsible for glucose uptake as measured by the IPGTT: adipose tissue growing (suggested by increased protein synthesis and degradation and mTOR proliferation signaling upregulation)...
February 23, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473771/multi-omics-analysis-of-the-effects-of-soil-amendment-on-rapeseed-brassica-napus-l-photosynthesis-under-drip-irrigation-with-brackish-water
#18
JOURNAL ARTICLE
Ziwei Li, Hua Fan, Le Yang, Shuai Wang, Dashuang Hong, Wenli Cui, Tong Wang, Chunying Wei, Yan Sun, Kaiyong Wang, Yantao Liu
Drip irrigation with brackish water increases the risk of soil salinization while alleviating water shortage in arid areas. In order to alleviate soil salinity stress on crops, polymer soil amendments are increasingly used. But the regulation mechanism of a polymer soil amendment composed of polyacrylamide polyvinyl alcohol, and manganese sulfate (PPM) on rapeseed photosynthesis under drip irrigation with different types of brackish water is still unclear. In this field study, PPM was applied to study the responses of the rapeseed ( Brassica napus L...
February 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38472772/exploring-the-antibacterial-potential-and-underlying-mechanisms-of-prunella-vulgaris-l-on-methicillin-resistant-staphylococcus-aureus
#19
JOURNAL ARTICLE
Ziyin Li, Qiqi He, Feifei Xu, Xinxin Yin, Zhuofan Guan, Jia Song, Zhini He, Xingfen Yang, Chen Situ
Prunella vulgaris L. (PV) is a widely distributed plant species, known for its versatile applications in both traditional and contemporary medicine, as well as in functional food development. Despite its broad-spectrum antimicrobial utility, the specific mechanism of antibacterial action remains elusive. To fill this knowledge gap, the present study investigated the antibacterial properties of PV extracts against methicillin-resistant Staphylococcus aureus (MRSA) and assessed their mechanistic impact on bacterial cells and cellular functions...
February 22, 2024: Foods (Basel, Switzerland)
https://read.qxmd.com/read/38467616/mitochondrial-metabolism-sustains-cd8-t-cell-migration-for-an-efficient-infiltration-into-solid-tumors
#20
JOURNAL ARTICLE
Luca Simula, Mattia Fumagalli, Lene Vimeux, Irena Rajnpreht, Philippe Icard, Gary Birsen, Dongjie An, Frédéric Pendino, Adrien Rouault, Nadège Bercovici, Diane Damotte, Audrey Lupo-Mansuet, Marco Alifano, Marie-Clotilde Alves-Guerra, Emmanuel Donnadieu
The ability of CD8+ T cells to infiltrate solid tumors and reach cancer cells is associated with improved patient survival and responses to immunotherapy. Thus, identifying the factors controlling T cell migration in tumors is critical, so that strategies to intervene on these targets can be developed. Although interstitial motility is a highly energy-demanding process, the metabolic requirements of CD8+ T cells migrating in a 3D environment remain unclear. Here, we demonstrate that the tricarboxylic acid (TCA) cycle is the main metabolic pathway sustaining human CD8+ T cell motility in 3D collagen gels and tumor slices while glycolysis plays a more minor role...
March 11, 2024: Nature Communications
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