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https://www.readbyqxmd.com/read/28813453/metabolomics-reveals-that-vine-tea-ampelopsis-grossedentata-prevents-high-fat-diet-induced-metabolism-disorder-by-improving-glucose-homeostasis-in-rats
#1
Wenting Wan, Baoping Jiang, Le Sun, Lijia Xu, Peigen Xiao
BACKGROUND: Vine tea (VT), derived from Ampelopsis grossedentata (Hand.-Mazz.) W.T. Wang, is an alternative tea that has been consumed widely in south China for hundreds of years. It has been shown that drinking VT on a daily basis improves hyperlipidemia and hyperglycemia. However, little is known about the preventive functions of VT for metabolic dysregulation and the potential pathological mechanisms involved. This paper elucidates the preventive effects of VT on the dysregulation of lipid and glucose metabolism using rats maintained on a high-fat-diet (HFD) in an attempt to explain the potential mechanisms involved...
2017: PloS One
https://www.readbyqxmd.com/read/28811348/a-critical-role-of-glutamine-and-asparagine-%C3%AE-nitrogen-in-nucleotide-biosynthesis-in-cancer-cells-hijacked-by-an-oncogenic-virus
#2
Ying Zhu, Tingting Li, Suzane Ramos da Silva, Jae-Jin Lee, Chun Lu, Hyungjin Eoh, Jae U Jung, Shou-Jiang Gao
While glutamine is a nonessential amino acid that can be synthesized from glucose, some cancer cells primarily depend on glutamine for their growth, proliferation, and survival. Numerous types of cancer also depend on asparagine for cell proliferation. The underlying mechanisms of the glutamine and asparagine requirement in cancer cells in different contexts remain unclear. In this study, we show that the oncogenic virus Kaposi's sarcoma-associated herpesvirus (KSHV) accelerates the glutamine metabolism of glucose-independent proliferation of cancer cells by upregulating the expression of numerous critical enzymes, including glutaminase 2 (GLS2), glutamate dehydrogenase 1 (GLUD1), and glutamic-oxaloacetic transaminase 2 (GOT2), to support cell proliferation...
August 15, 2017: MBio
https://www.readbyqxmd.com/read/28808378/rutamarin-an-active-constituent-from-ruta-angustifolia-pers-induced-apoptotic-cell-death-in-the-ht29-colon-adenocarcinoma-cell-line
#3
Shafinah Ahmad Suhaimi, Sok Lai Hong, Sri Nurestri Abdul Malek
BACKGROUND: Ruta angustifolia Pers. is a perennial herb that is cultivated worldwide, including Southeast Asia, for the treatment of various diseases as traditional medicine. OBJECTIVE: The purpose of the study was to identify an active principle of R. angustifolia and to investigate its effect on the HT29 cell death. MATERIALS AND METHODS: The methanol and fractionated extracts (hexane, chloroform, ethyl acetate, and water) of R. angustifolia Pers...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28806601/influence-of-sublethal-doses-of-acetamiprid-and-halosulfuron-methyl-on-metabolites-of-zebra-fish-brachydanio-rerio
#4
Hong Zhang, Lijuan Zhao
Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to investigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio)...
August 4, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28802408/down-regulation-of-malate-synthase-in-mycobacterium-tuberculosis-h37ra-leads-to-reduced-stress-tolerance-persistence-and-survival-in-macrophages
#5
Kumar Sachin Singh, Rishabh Sharma, Deepa Keshari, Nirbhay Singh, Sudheer Kumar Singh
Malate synthase is a condensing enzyme responsible for conversion of glyoxylate to malate in the presence of acetyl-CoA. This reaction helps in bypassing the TCA cycle reactions involving carbon loss and leads to diverting some of the carbon skeletons to gluconeogenic events while rest can continue to provide TCA cycle intermediates. Malate synthase (GlcB) is encoded by MRA_1848 of Mycobacterium tuberculosis H37Ra (Mtb-Ra). We developed a knockdown (KD) Mtb-Ra strain by down-regulating GlcB. The survival studies suggested increased susceptibility to oxidative and nitrosative stress as well as to rifampicin...
September 2017: Tuberculosis
https://www.readbyqxmd.com/read/28801776/effects-of-training-status-on-pdh-regulation-in-human-skeletal-muscle-during-exercise
#6
Anders Gudiksen, Lærke Bertholdt, Tomasz Stankiewicz, Jonas Tybirk, Peter Plomgaard, Jens Bangsbo, Henriette Pilegaard
Pyruvate dehydrogenase (PDH) is the gateway enzyme for carbohydrate-derived pyruvate feeding into the TCA cycle. PDH may play a central role in regulating substrate shifts during exercise, but the influence of training state on PDH regulation during exercise is not fully elucidated. The purpose of this study was to investigate the impact of training state on post-translational regulation of PDHa activity during submaximal and exhaustive exercise. Eight untrained and nine endurance exercise-trained healthy male subjects performed incremental exercise on a cycle ergometer: 40 min at 50% incremental peak power output (IPPO), 10 min at 65% (IPPO), followed by 80% (IPPO) until exhaustion...
August 11, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28800966/reconstruction-of-a-metabolic-regulatory-network-in-escherichia-coli-for-purposeful-switching-from-cell-growth-mode-to-production-mode-in-direct-gaba-fermentation-from-glucose
#7
Yuki Soma, Yuri Fujiwara, Takuya Nakagawa, Keigo Tsuruno, Taizo Hanai
γ-aminobutyric acid (GABA) is a drug and functional food additive and is used as a monomer for producing the biodegradable plastic, polyamide 4. Recently, direct GABA fermentation from glucose has been developed as an alternative to glutamate-based whole cell bioconversion. Although total productivity in fermentation is determined by the specific productivity and cell amount responsible for GABA production, the optimal metabolic state for GABA production conflicts with that for bacterial cell growth. Herein, we demonstrated metabolic state switching from the cell growth mode based on the metabolic pathways of the wild type strain to a GABA production mode based on a synthetic metabolic pathway in Escherichia coli through rewriting of the metabolic regulatory network and pathway engineering...
August 8, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28797234/succinyl-proteome-profiling-of-dendrobium-officinale-an-important-traditional-chinese-orchid-herb-revealed-involvement-of-succinylation-in-the-glycolysis-pathway
#8
Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia Shen
BACKGROUND: Lysine succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. RESULTS: In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine succinylation sites in 207 proteins were identified...
August 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28780297/respiration-disruption-and-detoxification-at-the-protein-expression-levels-in-the-pacific-oyster-crassostrea-gigas-under-zinc-exposure
#9
Jie Meng, Wen-Xiong Wang, Li Li, Guofan Zhang
The Pacific oyster (Crassostrea gigas) can accumulate high levels of zinc (Zn) without obvious toxicity, but the related molecular mechanisms are largely unknown. In the present study, C. gigas were exposed to excess Zn for 9days and the differentially expressed proteins (DEPs) were examined using the isobaric tags for relative and absolute quantitation (iTRAQ) method. In total, 2667 proteins containing at least two peptides and detected in both replicates were used for proteomic analysis. Among these DEPs, 332 were up-regulated and 282 were down-regulated...
July 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28776265/traumatic-brain-injury-alters-the-metabolism-and-facilitates-alzheimer-s-disease-in-a-murine-model
#10
Dandan Lou, Yao Du, Daochao Huang, Fang Cai, Yun Zhang, Tinyu Li, Weihui Zhou, Hongchang Gao, Weihong Song
A majority of Alzheimer's disease (AD) cases are sporadic without known cause. People who suffered from traumatic brain injury (TBI) are more likely to develop neurodegeneration and cognitive impairments. However, the role of TBI in pathophysiology of AD remains elusive. The present study intended to explore the effect of TBI on metabolism and its role in AD pathogenesis. We subjected double transgenic AD model mice APP23/PS45 to TBI. We found that TBI promoted β-secretase cleavage of amyloid β precursor protein and amyloid β protein deposition, and exuberated the cognitive impairments in AD mouse models...
August 3, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28769919/cellular-response-to-ciprofloxacin-in-low-level-quinolone-resistant-escherichia-coli
#11
Jesús Machuca, Esther Recacha, Alejandra Briales, Paula Díaz-de-Alba, Jesús Blazquez, Álvaro Pascual, José-Manuel Rodríguez-Martínez
Bactericidal activity of quinolones has been related to a combination of DNA fragmentation, reactive oxygen species (ROS) production and programmed cell death (PCD) systems. The underlying molecular systems responsible for reducing bactericidal effect during antimicrobial therapy in low-level quinolone resistance (LLQR) phenotypes need to be clarified. To do this and also define possible new antimicrobial targets, the transcriptome profile of isogenic Escherichia coli harboring quinolone resistance mechanisms in the presence of a clinical relevant concentration of ciprofloxacin was evaluated...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28767134/the-differential-impact-of-amino-acids-on-oxphos-system-activity-following-carbohydrate-starvation-in-arabidopsis-cell-suspensions
#12
João Henrique F Cavalcanti, Carla G S Quinhones, Peter Schertl, Danielle S Brito, Holger Eubel, Tatjana Hildebrandt, Adriano Nunes-Nesi, Hans-Peter Braun, Wagner L Araújo
Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid (TCA) cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons via the ETF/ETFQO complex to the mitochondrial electron transport chain (mETC). Given to this, here we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids...
August 2, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28766915/metabolomics-analysis-reveals-distinct-profiles-of-nonmuscle-invasive-and-muscle-invasive-bladder-cancer
#13
Divya Sahu, Yair Lotan, Bryan Wittmann, Bruce Neri, Donna E Hansel
Urothelial carcinoma is the most common form of bladder cancer, but pathway changes that occur with stage-wise progression have not been well defined. We used a metabolomics approach to identify potential metabolic pathways uniquely altered in normal urothelium, nonmuscle-invasive bladder cancer (NMIBC), and muscle-invasive bladder cancer (MIBC). We performed global metabolomic profiling using GC-mass spectrometry (MS) and LC-MS platforms to identify metabolite signatures between normal urothelium and high-grade urothelial carcinoma of different stages...
August 1, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28764799/metabolomics-study-of-the-therapeutic-mechanism-of-schisandra-chinensis-lignans-in-diet-induced-hyperlipidemia-mice
#14
Jing-Hui Sun, Xu Liu, Li-Xin Cong, He Li, Cheng-Yi Zhang, Jian-Guang Chen, Chun-Mei Wang
BACKGROUND: Schisandra, a globally distributed plant, has been widely applied for the treatment of diseases such as hyperlipidemia, fatty liver and obesity in China. In the present work, a rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS)-based metabolomics was conducted to investigate the intervention effect of Schisandra chinensis lignans (SCL) on hyperlipidemia mice induced by high-fat diet (HFD). METHODS: Hyperlipidemia mice were orally administered with SCL (100 mg/kg) once a day for 4 weeks...
August 1, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28763759/metabolomics-analysis-of-danggui-sini-decoction-on-treatment-of-collagen-induced-arthritis-in-rats
#15
Bang Cheng, Hua Zheng, Fang Wu, Jinxia Wu, Xuwen Liu, Chaoling Tang, Shiyin Lu, Zhaoni Chen, Fangming Song, Junxiang Ruan, Hongye Zhang, Hui Song, Wei Liu, Hongwei Guo, Yonghong Liang, Zhiheng Su
Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by persistent joint inflammation leading to bone and cartilage damage and even disability. However, the pathogenesis of RA is multi-factorial and to a large degree, remains unknown. Danggui Sini decoction (DSD), a traditional Chinese medicine (TCM) formula, has been widely used as a remedy for rheumatoid arthritis (RA) in recent years. In our study, (1)H-nuclear magnetic resonance ((1)H NMR) based metabolomics analysis of 7 potential biomarkers, including taurine (1), urea (2), betaine (3), pyruvate (4), hippurate (5), succinate (6) and acetone (7) was performed to investigate the progression of RA and assess the efficacy of DSD in collagen-induced arthritis (CIA) rats...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28763459/metabolic-engineering-of-cho-cells-for-the-development-of-a-robust-protein-production-platform
#16
Sanjeev Kumar Gupta, Santosh K Srivastava, Ankit Sharma, Vaibhav H H Nalage, Darshita Salvi, Hiralal Kushwaha, Nikhil B Chitnis, Pratyoosh Shukla
Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pharmaceutical production. A major challenge in metabolic engineering is to balance the flux of the tuned heterogonous metabolic pathway and achieve efficient metabolic response in a mammalian cellular system. Pyruvate carboxylase is an important network element for the cytoplasmic and mitochondrial metabolic pathway and efficiently contributes in enhancing the energy metabolism. The lactate accumulation in cell culture can be reduced by re-wiring of the pyruvate flux in engineered cells...
2017: PloS One
https://www.readbyqxmd.com/read/28759723/physiological-and-transcriptomic-analysis-validates-previous-findings-of-changes-in-primary-metabolism-for-the-production-of-phenolic-antioxidants-in-wounded-carrots
#17
Cong Han, Peng Jin, Meilin Li, Lei Wang, Yonghua Zheng
Wounding induces the accumulation of phenolic compounds in carrot. This study uses physiological and transcriptomic analysis to validate previous findings relating primary metabolism and secondary metabolites in wounded carrots. Our data confirmed that increased wounding intensity strengthened the accumulation of phenolics accompanied by enhancing respiration and showed the loss of fructose and glucose and the increase of energy status in carrots. In addition, transcriptomic evaluation of shredded carrots indicated that the respiratory metabolism, sugar metabolism, energy metabolism, and phenolic biosynthesis related pathways, such as "citrate cycle (TCA cycle)", "oxidative phosphorylation" and "phenylpropanoid biosynthesis", were activated by wounding...
August 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28758404/lycopene-prevents-mitochondrial-dysfunction-during-d-galactosamine-lipopolysaccharide-induced-fulminant-hepatic-failure-in-albino-rats
#18
Sheik Abdulazeez Sheriff, Shaikhussain Shaik Ibrahim, Thiruvengadam Devaki, Sandipan Chakraborty, Subhash Agarwal, Horacio Perez-Sanchez
Fulminant hepatic failure (FHF) is a serious health issue, with a high mortality rate and characterized by severe perturbation in mitochondrial function. D-Galactosamine/lipopolysaccharide (D-GalN/LPS) induced FHF is a renowned model to evaluate the efficacy of hepatoprotective agents. Lycopene is an antioxidant and phytonutrient from carotenoid family. The health benefits of lycopene are prominent against cancer, cardiovascular, lung, liver, and skin problems. Recent studies have demonstrated the hepatoprotective, antidyslipidemic, and antioxidant roles of lycopene...
July 31, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28755490/metabolic-engineering-of-isopropyl-alcohol-producing-escherichia-coli-strains-with-13-c-metabolic-flux-analysis
#19
Nobuyuki Okahashi, Fumio Matsuda, Katsunori Yoshikawa, Tomokazu Shirai, Yoshiko Matsumoto, Mitsufumi Wada, Hiroshi Shimizu
Metabolic engineering of isopropyl alcohol (IPA)-producing E. coli strains was conducted along with (13) C-metabolic flux analysis (MFA). A metabolically engineered E. coli strain expressing the adc gene derived from Clostridium acetobutylicum and the IPADH gene from C. beijerinckii did not produce IPA during its exponential growth phase in the aerobic batch culture. (13) C-MFA was carried out, and revealed a deficiency in NADPH regeneration for IPA production in growth phase. Based on these findings, we used nitrogen-starved culture conditions to reduce NADPH consumption for biomass synthesis...
July 29, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28752265/the-augmented-lipid-productivity-in-an-emerging-oleaginous-model-alga-coccomyxa-subellipsoidea-by-nitrogen-manipulation-strategy
#20
Chao Wang, Zhenyao Wang, Fei Luo, Yuqin Li
The lipid productivity controlled by both of biomass and lipid content was really crucial for economic-feasibility of microalgae-based biofuels production. This study attempted at augmenting lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by different nitrogen manipulation including one-stage continuous N-sufficiency (OCNS), N-deprivation (OCND), N-limitation (OCNL), and also two-stage batch N-starvation (TBNS). Amongst four tested nitrogen manipulation strategies, OCNS performed remarkable promoting effect on cell metabolic growth and the maximum biomass was achieved by 7...
August 2017: World Journal of Microbiology & Biotechnology
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