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https://www.readbyqxmd.com/read/28062031/how-to-measure-load-dependent-kinetics-of-individual-motor-molecules-without-a-force-clamp
#1
J Sung, K I Mortensen, J A Spudich, H Flyvbjerg
Single-molecule force spectroscopy techniques, including optical trapping, magnetic trapping, and atomic force microscopy, have provided unprecedented opportunities to understand biological processes at the smallest biological length scales. For example, they have been used to elucidate the molecular basis of muscle contraction and intracellular cargo transport along cytoskeletal filamentous proteins. Optical trapping is among the most sophisticated single-molecule techniques. With exceptionally high spatial and temporal resolutions, it has been extensively utilized to understand biological functions at the single molecule level, such as conformational changes and force-generation of individual motor proteins or force-dependent kinetics in molecular interactions...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28051333/optimal-loading-for-maximising-power-during-sled-resisted-sprinting
#2
Matt R Cross, Matt Brughelli, Pierre Samozino, Scott R Brown, Jean-Benoit Morin
Purpose We sought to ascertain whether force-velocity-power relationships could be compiled from a battery of sled-resisted over-ground sprints, and to clarify and compare the optimal loading conditions for maximising power production for different athlete cohorts. Methods Recreational mixed-sport athletes (n=12) and sprinters (n=15) performed multiple trials of maximal sprints unloaded and towing a selection of sled masses (20-120% body-mass). Velocity data were collected by sports radar, and kinetics at peak velocity were quantified using friction coefficients and aerodynamic drag...
January 4, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28044199/muscle-fascicle-shortening-behaviour-of-vastus-lateralis-during-a-maximal-force-velocity-test
#3
Hugo Hauraix, Sylvain Dorel, Giuseppe Rabita, Gaël Guilhem, Antoine Nordez
PURPOSE: Muscle fascicles-tendon interactions are the main determinant in production of high joint velocity. Currently, no study has investigated the muscle fascicles behaviour of knee extensor muscles until the highest reachable velocity (e.g., unloaded knee extension). We aimed to track the changes in vastus lateralis fascicles length during knee extensions to quantify muscle fascicles and tendinous tissues contributions to muscle-tendon unit shortening and to determine maximal muscle fascicles shortening velocity...
January 3, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28036211/load-response-of-the-flagellar-beat
#4
Gary S Klindt, Christian Ruloff, Christian Wagner, Benjamin M Friedrich
Cilia and flagella exhibit regular bending waves that perform mechanical work on the surrounding fluid, to propel cellular swimmers and pump fluids inside organisms. Here, we quantify a force-velocity relationship of the beating flagellum, by exposing flagellated Chlamydomonas cells to controlled microfluidic flows. A simple theory of flagellar limit-cycle oscillations, calibrated by measurements in the absence of flow, reproduces this relationship quantitatively. We derive a link between the energy efficiency of the flagellar beat and its ability to synchronize to oscillatory flows...
December 16, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/28027462/the-influence-of-asymmetric-force-requirements-on-a-multi-frequency-bimanual-coordination-task
#5
Deanna M Kennedy, Joohyun Rhee, Judith Jimenez, Charles H Shea
An experiment was designed to determine the impact of the force requirements on the production of bimanual 1:2 coordination patterns requiring the same (symmetric) or different (asymmetric) forces when Lissajous displays and goal templates are provided. The Lissajous displays have been shown to minimize the influence of attentional and perceptual constraints allowing constraints related to neural crosstalk to be more clearly observed. Participants (N=20) were randomly assigned to a force condition in which the left or right limb was required to produce more force than the contralateral limb...
December 24, 2016: Human Movement Science
https://www.readbyqxmd.com/read/27988910/importance-of-contraction-history-on-muscle-force-of-porcine-urinary-bladder-smooth-muscle
#6
Robin Menzel, Markus Böl, Tobias Siebert
INTRODUCTION: The purpose of this study was to provide a comprehensive dataset of porcine urinary bladder smooth muscle properties. Particularly, the history dependence of force production, namely force depression (FD) following shortening and force enhancement (FE) following stretch, was analysed. During active micturition, the circumference of the urinary bladder changes enormously. Thus, FD might be an important phenomenon during smooth muscle contraction. MATERIALS AND METHODS: Electrically stimulated, intact urinary bladder strips from pigs (n = 10) were suspended in an aerated-filled organ bath, and different isometric, isotonic, and isokinetic contraction protocols were performed to determine the force-length and the force-velocity relation...
December 17, 2016: International Urology and Nephrology
https://www.readbyqxmd.com/read/27918682/changes-in-the-force-velocity-mechanical-profile-after-short-resistance-training-programmes-differing-in-set-configurations
#7
Eliseo Iglesias-Soler, Miguel Fernández-Del-Olmo, Xián Mayo, Juan Fariñas, Dan Río-Rodríguez, Eduardo Carballeira, Elvis A Carnero, Robert A Standley, Manuel A Giráldez-García, Xurxo Dopico-Calvo, Jose Luis Tuimil
The main aim of this study was to analyse the effect of resistance training programs differing in set configuration on mechanical force-velocity profiles. Thirteen participants performed ten unilateral knee extension training sessions over 5 weeks. Each limb was randomised to one of the following set configurations: traditional (4 sets of 8 repetitions at maximum intended velocity, 10RM load, 3-min pause between sets), and inter-repetition rest (32 maximum intended velocity repetitions, 10RM load, 17.4 s of rest between each repetition)...
December 5, 2016: Journal of Applied Biomechanics
https://www.readbyqxmd.com/read/27915070/vicarious-ratings-of-social-touch-reflect-the-anatomical-distribution-velocity-tuning-of-c-tactile-afferents-a-hedonic-homunculus
#8
Susannah C Walker, Paula D Trotter, Andy Woods, Francis McGlone
A subclass of C-fibres, C-tactile afferents (CTs), have been discovered which respond preferentially to low force/velocity stroking touch, that is typically perceived as pleasant. Molecular genetic visualization of these low-threshold mechanosensitive C-fibres (CLTMs) in mice revealed a denser distribution in dorsal than ventral thoracic sites, scattered distal limb innervation and a complete absence from glabrous paw skin (Liu et al., 2007). Here we used third-party ratings to examine whether affective responses to social touch reflect the anatomical distribution and velocity tuning of CTs...
November 30, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27913559/physical-activity-as-intervention-for-age-related-loss-of-muscle-mass-and-function-protocol-for-a-randomised-controlled-trial-the-lisa-study
#9
Christian Skou Eriksen, Ellen Garde, Nina Linde Reislev, Cathrine Lawaetz Wimmelmann, Theresa Bieler, Andreas Kraag Ziegler, Anne Theil Gylling, Kasper Juel Dideriksen, Hartwig Roman Siebner, Erik Lykke Mortensen, Michael Kjaer
INTRODUCTION: Physical and cognitive function decline with age, accelerating during the 6th decade. Loss of muscle power (force×velocity product) is a dominant physical determinant for loss of functional ability, especially if the lower extremities are affected. Muscle strength training is known to maintain or even improve muscle power as well as physical function in older adults, but the optimal type of training for beneficial long-term training effects over several years is unknown...
December 2, 2016: BMJ Open
https://www.readbyqxmd.com/read/27896682/methods-of-power-force-velocity-profiling-during-sprint-running-a-narrative-review
#10
REVIEW
Matt R Cross, Matt Brughelli, Pierre Samozino, Jean-Benoit Morin
The ability of the human body to generate maximal power is linked to a host of performance outcomes and sporting success. Power-force-velocity relationships characterize limits of the neuromuscular system to produce power, and their measurement has been a common topic in research for the past century. Unfortunately, the narrative of the available literature is complex, with development occurring across a variety of methods and technology. This review focuses on the different equipment and methods used to determine mechanical characteristics of maximal exertion human sprinting...
November 28, 2016: Sports Medicine
https://www.readbyqxmd.com/read/27864330/three-dimensional-stochastic-model-of-actin-myosin-binding-in-the-sarcomere-lattice
#11
Srboljub M Mijailovich, Oliver Kayser-Herold, Boban Stojanovic, Djordje Nedic, Thomas C Irving, Michael A Geeves
The effect of molecule tethering in three-dimensional (3-D) space on bimolecular binding kinetics is rarely addressed and only occasionally incorporated into models of cell motility. The simplest system that can quantitatively determine this effect is the 3-D sarcomere lattice of the striated muscle, where tethered myosin in thick filaments can only bind to a relatively small number of available sites on the actin filament, positioned within a limited range of thermal movement of the myosin head. Here we implement spatially explicit actomyosin interactions into the multiscale Monte Carlo platform MUSICO, specifically defining how geometrical constraints on tethered myosins can modulate state transition rates in the actomyosin cycle...
December 2016: Journal of General Physiology
https://www.readbyqxmd.com/read/27824440/nonlinear-response-of-inertial-tracers-in-steady-laminar-flows-differential-and-absolute-negative-mobility
#12
A Sarracino, F Cecconi, A Puglisi, A Vulpiani
We study the mobility and the diffusion coefficient of an inertial tracer advected by a two-dimensional incompressible laminar flow, in the presence of thermal noise and under the action of an external force. We show, with extensive numerical simulations, that the force-velocity relation for the tracer, in the nonlinear regime, displays complex and rich behaviors, including negative differential and absolute mobility. These effects rely upon a subtle coupling between inertia and applied force that induces the tracer to persist in particular regions of phase space with a velocity opposite to the force...
October 21, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27806673/sprint-performance-and-mechanical-outputs-computed-with-an-iphone-app-comparison-with-existing-reference-methods
#13
Natalia Romero-Franco, Pedro Jiménez-Reyes, Adrián Castaño-Zambudio, Fernando Capelo-Ramírez, Juan José Rodríguez-Juan, Jorge González-Hernández, Francisco Javier Toscano-Bendala, Víctor Cuadrado-Peñafiel, Carlos Balsalobre-Fernández
The purpose of this study was to assess validity and reliability of sprint performance outcomes measured with an iPhone application (named: MySprint) and existing field methods (i.e. timing photocells and radar gun). To do this, 12 highly trained male sprinters performed 6 maximal 40-m sprints during a single session which were simultaneously timed using 7 pairs of timing photocells, a radar gun and a newly developed iPhone app based on high-speed video recording. Several split times as well as mechanical outputs computed from the model proposed by Samozino et al...
November 3, 2016: European Journal of Sport Science
https://www.readbyqxmd.com/read/27705034/the-high-pull-exercise-a-comparison-between-a-versapulley-flywheel-device-and-the-free-weight
#14
F Javier Núñez, Luis J Suarez-Arrones, Paul Cater, Alberto Mendez-Villanueva
The aim of this study was to examine the kinematics and kinetics (force, velocity and acceleration) and blood lactate concentration of the Versapulley (VP) device in comparison with free weight (FW) exercise at a similar external load. Fifteen rugby players randomly performed two training sessions of 6 sets of 6 repetitions with 20 s of recovery in between sets of the high pull exercise with the VP and the FW. Both training sessions were separated by 72 h. Barbell displacement (cm.), peak velocity (m/s), peak acceleration (m/s(2)), mean propulsive velocity (m/s), mean propulsive acceleration (m/s(2)), propulsive phase (%), mean and maximal force (Newtons) were continuously recorded during each repetition...
September 6, 2016: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/27676816/hamstring-injury-prevention-contributions-of-biomechanics
#15
Pascal Edouard, Pierre Samozino, Romain Slotala, Jurdan Mendiguchia, Jean-Benoit Morin
Hamstring muscle injury is the main injury related to athletics, but also other sports including acceleration and sprints, with important consequences (time loss of sport and re-injury risk). In a prevention approach, a better knowledge of risk factors and mechanisms of these lesions seem relevant, and it can pass through a better understanding of the biomechanical and muscular determinants of performance in sprint and acceleration. The ability to produce a horizontal force on the ground [i.e. orienting the total push backward (or ground force reaction forward)], and to do so despite the increase in running speed, is an important determinant of sprint performance...
September 2016: Annals of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/27669187/muscle-specific-effective-mechanical-advantage-and-joint-impulse-in-weightlifting
#16
Kristof Kipp, Chad Harris
Lifting greater loads during weightlifting exercises may theoretically be achieved through increasing the magnitudes of net joint impulses or manipulating the joints' effective mechanical advantage (EMA). The purpose of this study was to investigate muscle-specific EMA and joint impulse as well as impulse-momentum characteristics of the lifter-barbell system across a range of external loads during the execution of the clean. Collegiate-level weightlifters performed sub-maximal cleans at 65, 75, and 85% of their 1-repetition maximum (1-RM) while data from a motion analysis system and a force plate were used to calculate lifter-barbell system impulse and velocity, as well as net extensor impulse generated at the hip, knee, and ankle joints and the EMA of the gluteus maximus, hamstrings, quadriceps, and triceps surae muscles...
September 21, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27618131/the-high-pull-exercise-a-comparison-between-a-versapulley-flywheel-device-and-the-free-weight
#17
F Javier Núñez, Luis J Suarez-Arrones, Paul Cater, Alberto Mendez-Villanueva
The aim of this study was to examine the kinematics and kinetics (force, velocity and acceleration) and blood lactate concentration of the Versapulley (VP) device in comparison with free weight (FW) exercise at a similar external load. Fifteen rugby players randomly performed two training sessions of 6 sets of 6 repetitions with 20 s of recovery in between sets of the high pull exercise with the VP and the FW. Both training sessions were separated by 72 h. Barbell displacement (cm.), peak velocity (m/s), peak acceleration (m/s2), mean propulsive velocity (m/s), mean propulsive acceleration (m/s2), propulsive phase (%), mean and maximal force (Newtons) were continuously recorded during each repetition...
September 6, 2016: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/27582260/bilateral-deficit-in-maximal-force-production
#18
REVIEW
Jakob Škarabot, Neil Cronin, Vojko Strojnik, Janne Avela
The bilateral deficit phenomenon, characterized by a reduction in the amount of force from a single limb during maximal bilateral actions, has been shown in various movement tasks, contraction types and different populations. However, bilateral deficit appears to be an inconsistent phenomenon, with high variability in magnitude and existence, and seems to be plastic, as bilateral facilitation has also been shown to occur. Furthermore, many mechanisms underlying this phenomenon have been proposed over the years, but still remain largely unknown...
December 2016: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/27577950/1rm-prediction-a-novel-methodology-based-on-the-force-velocity-and-load-velocity-relationships
#19
Pietro Picerno, Danilo Iannetta, Stefania Comotto, Marco Donati, Fabrizio Pecoraro, Mounir Zok, Giorgio Tollis, Marco Figura, Carlo Varalda, Davide Di Muzio, Federica Patrizio, Maria Francesca Piacentini
PURPOSE: This study aimed to evaluate the accuracy of a novel approach for predicting the one-repetition maximum (1RM). The prediction is based on the force-velocity and load-velocity relationships determined from measured force and velocity data collected during resistance-training exercises with incremental submaximal loads. 1RM was determined as the load corresponding to the intersection of these two curves, where the gravitational force exceeds the force that the subject can exert...
October 2016: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/27548784/acute-kinematic-and-kinetic-adaptations-to-wearable-resistance-during-sprint-acceleration
#20
Paul Macadam, Kim D Simperingham, John B Cronin
Wearable resistance (WR) in the form of weighted vests and shorts enables movement specific sprint running to be performed under load. The purpose of this study was to determine the acute changes in kinematics and kinetics when an additional load equivalent to 3% body mass (BM) was attached to the anterior or posterior surface of the lower limbs during sprint running. Nineteen male rugby athletes (age: 19.7 ± 2.3 years; body mass: 96.1 ± 16.5 kg; height: 181 ± 6.5 cm) volunteered to participate in the study...
August 18, 2016: Journal of Strength and Conditioning Research
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