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https://www.readbyqxmd.com/read/29347914/in-depth-proteomic-analysis-of-boar-spermatozoa-through-shotgun-and-gel-based-methods
#1
Jean M Feugang, Shengfa F Liao, Scott T Willard, Peter L Ryan
BACKGROUND: Mature spermatozoa contain numerous epididymal and seminal plasma proteins, which full identification through high-throughput technologies may allow for a better understanding of the sperm biology. Therefore, we conducted a global proteomic analysis of boar spermatozoa through shotgun and gel-based methodologies. RESULTS: The total proteins were extracted from mature spermatozoa and subjecsted to proteome analyses. Functional analyses of gene ontology representations and pathway enrichments were conducted on the shotgun dataset, followed by immunology and gene expression validations...
January 18, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29344801/toxoplasma-gondii-in-sympatric-domestic-and-wild-ungulates-in-the-mediterranean-ecosystem
#2
S Almería, O Cabezón, J Paniagua, D Cano-Terriza, S Jiménez-Ruiz, A Arenas-Montes, J P Dubey, I García-Bocanegra
Toxoplasma gondii is a zoonotic protozoan of worldwide distribution. The present study provides information on risk factors affecting T. gondii infection in domestic and free-ranging wild ungulates sharing habitats in Mediterranean ecosystems in Spain. Serum samples from 482 extensively reared domestic ruminants and 2351 wild ungulates were tested for T. gondii antibodies using the modified agglutination test (MAT, cut-off 1:25). Toxoplasma gondii seroprevalence was 41.2% of 194 sheep, 18.6% of 199 cattle and 5...
January 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29333626/effect-of-astaxanthin-on-the-quality-of-boar-sperm-stored-at-17%C3%A2-c-incubated-at-37%C3%A2-c-or-under-in-vitro-conditions
#3
A Basioura, C M Boscos, I Parrilla, G Tsousis, I A Tsakmakidis
The aim of the study was to investigate the effect of the antioxidant astaxanthin on boar semen. Twenty ejaculates from 10 boars (two ejaculates/boar) were extended and split in three groups: semen control (SC), solvent control (C; semen with dimethyl sulfoxide, the diluent of astaxanthin) and semen with astaxanthin (A) in concentration 0.5 μmol/L. Sperm quality parameters (motility and kinetics, morphology, viability, functional integrity of sperm plasma membrane by Hypo-Osmotic Swelling Test [HOST] and DNA integrity) were assessed at 0, 24 and 48 hr of storage at 17°C (experiment I), before (0 hr) and after (1 hr) of sperm thermal resistance assay at 37°C (experiment II) and finally before (0 hr) and after (1 hr) sperm in vitro incubation (38...
January 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29330895/effect-of-local-scrotal-heating-on-the-expression-of-tight-junction-associated-molecule-occludin-in-boar-testes
#4
Z Zhang, X Fan, H Xi, R Ji, H Shen, A Shi, J He
The aim of this study was to determine whether local scrotal heating (42°C, for 1 hr) had an effect on the expression of tight junction (TJ)-associated molecule Occludin in boar testes. Adult boars (Landrace, n = 6) were used and randomly divided into two groups (n = 3 each). Three boars were given local scrotal exposure to 42°C for approximately 1 h with a home-made electric blanket of controlled temperature as local scrotal heating group, the other three boars received no heat treatment and were left at standard room temperature as control group...
January 12, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29329820/increased-testicular-estradiol-during-the-neonatal-interval-reduces-sertoli-cell-numbers
#5
Trish Berger, Barbara J Nitta-Oda
Previous studies have demonstrated that reducing endogenous testicular estradiol in neonatal boars would stimulate increased proliferation of Sertoli cells during the neonatal interval. The objective of this experiment was to determine if increasing testicular estradiol would have the opposite effect of reducing Sertoli cell numbers during the neonatal interval. Five littermate pairs of boars were evaluated with one littermate receiving a silastic implant containing estradiol and the second receiving only silastic at 1...
January 6, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29325489/creating-effective-biocontainment-facilities-and-maintenance-protocols-for-raising-specific-pathogen-free-severe-combined-immunodeficient-scid-pigs
#6
Ellis J Powell, Sara Charley, Adeline N Boettcher, Lisa Varley, Justin Brown, Martine Schroyen, Malavika K Adur, Susan Dekkers, Dean Isaacson, Mary Sauer, Joan Cunnick, N Matthew Ellinwood, Jason W Ross, Jack Cm Dekkers, Christopher K Tuggle
Severe combined immunodeficiency (SCID) is defined by the lack of an adaptive immune system. Mutations causing SCID are found naturally in humans, mice, horses, dogs, and recently in pigs, with the serendipitous discovery of the Iowa State University SCID pigs. As research models, SCID animals are naturally tolerant of xenotransplantation and offer valuable insight into research areas such as regenerative medicine, cancer therapy, as well as immune cell signaling mechanisms. Large-animal biomedical models, particularly pigs, are increasingly essential to advance the efficacy and safety of novel regenerative therapies on human disease...
January 1, 2018: Laboratory Animals
https://www.readbyqxmd.com/read/29325102/elevated-proportions-of-deleterious-genetic-variation-in-domestic-animals-and-plants
#7
Takashi Makino, Carl-Johan Rubin, Miguel Carneiro, Erik Axelsson, Leif Andersson, Matthew T Webster
A fraction of genetic variants segregating in any population are deleterious, which negatively impacts individual fitness. The domestication of animals and plants is associated with population bottlenecks and artificial selection, which are predicted to increase the proportion of deleterious variants. However, the extent to which this is a general feature of domestic species is unclear. Here we examine the effects of domestication on the prevalence of deleterious variation using pooled whole-genome resequencing data from five domestic animal species (dog, pig, rabbit, chicken and silkworm) and two domestic plant species (rice and soybean) compared to their wild ancestors...
January 9, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29322645/faecal-escherichia-coli-as-biological-indicator-of-spatial-interaction-between-domestic-pigs-and-wild-boar-sus-scrofa-in-corsica
#8
S A Barth, S Blome, D Cornelis, J Pietschmann, M Laval, O Maestrini, L Geue, F Charrier, E Etter, C Menge, M Beer, F Jori
On the Mediterranean island of Corsica, cohabitation between sympatric domestic pigs and Eurasian wild boar (Sus scrofa) is common and widespread and can facilitate the maintenance and dissemination of several pathogens detrimental for the pig industry or human health. In this study, we monitored a population of free-ranging domestic pigs reared in extensive conditions within a 800-ha property located in Central Corsica which was frequently visited by a sympatric population of wild boar between 2013 and 2015...
January 10, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29321862/assessing-the-utility-of-metabarcoding-for-diet-analyses-of-the-omnivorous-wild-pig-sus-scrofa
#9
Michael S Robeson, Kamil Khanipov, George Golovko, Samantha M Wisely, Michael D White, Michael Bodenchuck, Timothy J Smyser, Yuriy Fofanov, Noah Fierer, Antoinette J Piaggio
Wild pigs (Sus scrofa) are an invasive species descended from both domestic swine and Eurasian wild boar that was introduced to North America during the early 1500s. Wild pigs have since become the most abundant free-ranging exotic ungulate in the United States. Large and ever-increasing populations of wild pigs negatively impact agriculture, sport hunting, and native ecosystems with costs estimated to exceed $1.5 billion/year within the United States. Wild pigs are recognized as generalist feeders, able to exploit a broad array of locally available food resources, yet their feeding behaviors remain poorly understood as partially digested material is often unidentifiable through traditional stomach content analyses...
January 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29319413/molecular-evidence-of-rickettsia-slovaca-in-wild-boar-lice-in-northeastern-algeria
#10
Fayçal Zeroual, Hamza Leulmi, Ahmed Benakhla, Didier Raoult, Philippe Parola, Idir Bitam
Using molecular assays, Rickettsia slovaca, the agent of a spotted fever group rickettsia was assessed in 92/325 lice collected on 117 wild boars hunted in the far northeast of Algeria. Overall, 7/92 (7.61%) Haematopinus suis were found positive to R. slovaca. The presence of R. slovaca DNA on H. suis questions about the relationship that may exist between R. slovaca and lice.
January 10, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29311328/modeling-lethal-x-linked-genetic-disorders-in-pigs-with-ensured-fertility
#11
Hitomi Matsunari, Masahito Watanabe, Kazuaki Nakano, Shin Enosawa, Kazuhiro Umeyama, Ayuko Uchikura, Sayaka Yashima, Toru Fukuda, Nikolai Klymiuk, Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Valeri Zakhartchenko, Eckhard Wolf, Yutaka Hanazono, Masaki Nagaya, Akihiro Umezawa, Hiromitsu Nakauchi, Hiroshi Nagashima
Genetically engineered pigs play an indispensable role in the study of rare monogenic diseases. Pigs harboring a gene responsible for a specific disease can be efficiently generated via somatic cell cloning. The generation of somatic cell-cloned pigs from male cells with mutation(s) in an X chromosomal gene is a reliable and straightforward method for reproducing X-linked genetic diseases (XLGDs) in pigs. However, the severe symptoms of XLGDs are often accompanied by impaired growth and reproductive disorders, which hinder the reproduction of these valuable model animals...
January 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29310697/epidemiology-genetic-variants-and-clinical-course-of-natural-infections-with-anaplasma-phagocytophilum-in-a-dairy-cattle-herd
#12
Cornelia Silaghi, Marion Nieder, Carola Sauter-Louis, Gabriela Knubben-Schweizer, Kurt Pfister, Martin Pfeffer
BACKGROUND: Anaplasma phagocytophilum is an obligate intracellular, tick-transmitted bacterium that causes granulocytic anaplasmosis in humans and several mammalian species including domestic ruminants where it is called tick-borne fever (TBF). Different genetic variants exist but their impact with regard to putative differences in host associations and pathogenicity are not yet completely understood. METHODS: Natural infections with A. phagocytophilum in a dairy cattle herd in Germany were investigated over one pasture season by using serology, haematology, blood chemistry and polymerase chain reaction (PCR)...
January 8, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29305646/genetic-and-immunogenicity-analysis-of-porcine-circovirus-type-2-strains-isolated-in-central-china
#13
Chang Liu, Yunchao Liu, Hongying Chen, Hua Feng, Yumei Chen, Yinbiao Wang, Juan Wang, Dongmin Liu, Ruiguang Deng, Gaiping Zhang
Porcine circovirus type 2 (PCV2) is an economically important pathogen in domestic pigs and wild boars all around the world. To understand the molecular epidemiology of PCV2 strains circulating in central China and to provide a potential vaccine candidate strain, we analyzed the genetic variations of 46 PCV2 isolates circulating from 2009 to 2016 in Henan Province (24 detected in the field from 2009-2013 and 22 from 2013-2016) and evaluated the efficacy of an isolate as a vaccine candidate strain in a mouse model...
January 5, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29289610/body-composition-serum-lipid-levels-and-transcriptomic-characterization-in-the-adipose-tissue-of-male-pigs-in-response-to-sex-hormone-deficiency
#14
Yuchang Yao, Hongying Ma, Keliang Wu, Yonggang Shao, Wenpeng Han, Zhaowei Cai, Ningying Xu, Meiyu Qi, Chunjiang Zhao, Changxin Wu
It is known that the male hypogonadism plays an important role in regulating adipose metabolism. In the present study, fifteen pairs of full male sibs were divided into a castrated group and an intact group with a paired experiment design. The pigs were slaughtered at an age of 175days. The carcass characteristics and fat deposit of the studied animal were measured, and the hormone and serum lipid levels of the peripheral blood samples were determined, and the differentially expressed genes of the back fat between the two groups were screened with porcine genome array...
December 28, 2017: Gene
https://www.readbyqxmd.com/read/29277068/biological-effects-of-polyphenol-rich-extract-and-fractions-from-an-oenological-oak-derived-tannin-on-in%C3%A2-vitro-swine-sperm-capacitation-and-fertilizing-ability
#15
Marcella Spinaci, Vera Muccilli, Diego Bucci, Nunzio Cardullo, Beatrice Gadani, Corrado Tringali, Carlo Tamanini, Giovanna Galeati
Although excessive ROS levels induce sperm damage, sperm capacitation is an oxidative event that requires low amounts of ROS. As the antioxidant activity of the ethanol extract (TRE) of a commercial oenological tannin (Quercus robur toasted oak wood, Tan'Activ R®) and its four fractions (FA, FB, FC, FD) has been recently reported, the present study was set up to investigate the biological effects of TRE and its fractions in an in vitro model of sperm capacitation and fertilization. Boar sperm capacitation or gamete coincubation were performed in presence of TRE or its fractions (0, 1, 10, 100 μg/ml)...
December 14, 2017: Theriogenology
https://www.readbyqxmd.com/read/29274354/ochratoxin-a-exposure-decreased-sperm-motility-via-the-ampk-and-pten-signaling-pathways
#16
Tian-Yu Zhang, Rui-Ying Wu, Yong Zhao, Chun-Shuang Xu, Wei-Dong Zhang, Wei Ge, Jing Liu, Zhong-Yi Sun, Shu-Hua Zou, Wei Shen
Ochratoxin A (OTA), a common mycotoxin found in nature, has been implicated as effecting the function of male reproductive systems. OTA exposure has been shown to decrease sperm production and quality, however, the underlying mechanisms remain unknown. In the current investigation boar sperm exposed to 10 and 100μM OTA in vitro for 24h resulted in significantly decreased motility, in the 100μM OTA treatment group when compared with the control group. The level of reactive oxygen species (ROS) was significantly increased in both of the OTA treatment groups...
December 20, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29259947/prevalence-of-trichinella-spp-infections-in-hunted-wild-boars-in-northern-iran
#17
Ali Rostami, Hooshang Khazan, Bahram Kazemi, Eshrat Beigom Kia, Mojgan Bandepour, Niloofar Taghipour, Gholamreza Mowlavi
Background: Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the prevalence of Trichinella spp. infections in hunted wild boars in northern Iran. Methods: Thirty-five hunted wild boars were subjected in this study in 2015. All samples were examined by conventional artificial digestion method to detect of muscle larvae...
December 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29250528/questionnaire-based-assessment-of-wild-boar-domestic-pig-interactions-and-implications-for-disease-risk-management-in-corsica
#18
Ferran Jori, Anne Relun, Bastien Trabucco, François Charrier, Oscar Maestrini, David Chavernac, Daniel Cornelis, François Casabianca, Eric Marcel Charles Etter
Wild boars and domestic pigs belong to the same species (Sus scrofa). When sympatric populations of wild boars, feral pigs, and domestic pigs share the same environment, interactions between domestic and wild suids (IDWS) are suspected to facilitate the spread and maintenance of several pig pathogens which can impact on public health and pig production. However, information on the nature and factors facilitating those IDWS are rarely described in the literature. In order to understand the occurrence, nature, and the factors facilitating IDWS, a total of 85 semi-structured interviews were implemented face to face among 25 strict farmers, 20 strict hunters, and 40 hunting farmers in the main traditional pig-farming regions of Corsica, where IDWS are suspected to be common and widespread...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29249901/the-paradox-of-long-term-ungulate-impact-increase-of-plant-species-richness-in-a-temperate-forest
#19
Ondřej Vild, Radim Hédl, Martin Kopecký, Péter Szabó, Silvie Suchánková, Václav Zouhar
Questions: Did high densities of wild ungulates cause a decline in plant species richness in a temperate oakwood? How did species composition change after nearly five decades? Did ungulates facilitate the spread of ruderal species and supress endangered species? Did dispersal strategies play a role in these processes? Location: Krumlov Wood, SE Czech Republic. Methods: In 2012, we resampled 58 quasi-permanent vegetation plots first surveyed in 1960s...
April 2017: Applied Vegetation Science
https://www.readbyqxmd.com/read/29241320/influence-of-marinades-on-shear-force-structure-and-sensory-properties-of-home-style-jerky
#20
Joanna Żochowska-Kujawska, Marek Kotowicz, Kazimierz Lachowicz, Małgorzata Sobczak
BACKGROUND: Products from dried meat have been sold for many years as snack foods. The most important sensory attributes of this type of food are texture and flavour, determined by the selection of the raw material and the effect of numerous technological factors. The aim of the study was to investigate the structure, sen- sory and mechanical properties of meat snacks produced under non-commercial conditions using wild boar, roe deer and beef as raw material. METHODS: Jerky was prepared from Longissimus muscle treated (24 h, 4°C) with the follow- ing marinades before drying at 55°C for 6 h: (a) spices, (b) lemon & honey marinade, (c) marinating with balsamic vinegar marinade, (d) dipping into pineapple marinade, and (e) using ginger marinade...
October 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
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