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https://www.readbyqxmd.com/read/28646801/the-effects-of-exposure-to-low-frequency-electromagnetic-fields-on-male-fertility
#1
Mahsa Darbandi, Sara Darbandi, Ashok Agarwal, Ralf Henkle, Mohammad Reza Sadeghi
Context • People are increasingly exposed to low frequency (LF) electromagnetic fields (EMFs), mainly from electricity distribution networks and electronic devices. Critics of this widespread exposure believe that it can have detrimental effects on the human body. On the other hand, many in vivo and in vitro studies have claimed that low frequency electromagnetic therapy can function as a form of alternative medicine and that therapists can treat disease by applying electromagnetic radiation or pulsed EMFs to the body or cells...
June 23, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28633139/mitochondrial-metabolism-in-a-large-animal-model-of-huntington-disease-the-hunt-for-biomarkers-in-the-spermatozoa-of-presymptomatic-minipigs
#2
Jana Krizova, Hana Stufkova, Marie Rodinova, Monika Macakova, Bozena Bohuslavova, Daniela Vidinska, Jiri Klima, Zdenka Ellederova, Antonin Pavlok, David S Howland, Jiri Zeman, Jan Motlik, Hana Hansikova
BACKGROUND: Huntington disease (HD) is a fatal neurodegenerative disorder involving reduced muscle coordination, mental and behavioral changes, and testicular degeneration. In order to further clarify the decreased fertility and penetration ability of the spermatozoa of transgenic HD minipig boars (TgHD), we applied a set of mitochondrial metabolism (MM) parameter measurements to this promising biological material, which can be collected noninvasively in longitudinal studies. OBJECTIVE: We aimed to optimize methods for MM measurements in spermatozoa and to establish possible biomarkers of HD in TgHD spermatozoa expressing the N-terminal part of mutated human huntingtin...
June 21, 2017: Neuro-degenerative Diseases
https://www.readbyqxmd.com/read/28631185/the-increased-expression-of-follicle-stimulating-hormone-leads-to-a-decrease-of-fecundity-in-transgenic-large-white-female-pigs
#3
Kai Jiang, Pan Xu, Wanbo Li, Qiang Yang, Longyun Li, Chuanmin Qiao, Huanfa Gong, Hao Zheng, Zhimin Zhou, Hao Fu, Qiuyan Li, Yuyun Xing, Jun Ren
Follicle-stimulating hormone (FSH) is a pituitary gonadotropin regulating reproduction in mammals. Overexpression of the exogenous FSHα/β genes from Chinese Erhualian pigs improved female fecundity of transgenic (TG) mice and male spermatogenesis ability of Large White TG boars. Here, we investigated the impact of the exogenous FSHα/β genes on female reproductive performance of Large White TG pigs. First, we identified the integration site of the exogenous FSHα/β genes at 140,646,456 bp on chromosome 9 in these TG pigs using whole-genome sequencing...
June 19, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28628264/controlling-lactation-oestrus-the-final-frontier-for-breeding-herd-management
#4
REVIEW
William H E J van Wettere, Alice C Weaver, Emma C Greenwood, Robyn Terry, Paul E Hughes, Karen L Kind
Lactation anoestrus limits the flexibility of modern pig production systems such that any increase in lactation length reduces farrowing frequency, and thus profit. This review focuses on post-partum development of the sow's reproductive system, the physiology of lactation anoestrus and how it can be overcome, as well as the fertility of sows mated while lactating. The propensity for sows to ovulate spontaneously while lactating is high (24-31%), and a high proportion of sows will ovulate rapidly and synchronously in response to combinations of altered suckling (split weaning, interrupted suckling), daily boar contact, exogenous gonadotrophins, and group housing...
June 19, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28611113/active-paraoxonase-1-is-synthesised-throughout-the-internal-boar-genital-organs
#5
Isabel Barranco, Cristina Perez-Patiño, Asta Tvarijonaviciute, Inmaculada Parrilla, Alejandro Vicente-Carrillo, Manuel Alvarez-Rodriguez, Jose J Ceron, Emilio Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
The paraoxonase type 1 (PON1) is an enzyme with antioxidant properties recently identified in the seminal plasma (SP) of several species, including the porcine. The aims of the present study were to (1) describe the inmunohistochemical localization of PON1 in the genital organs of fertile boars and (2) evaluate the relationship among PON1 activity and high-density lipoprotein cholesterol (HDL-C) concentration in fluids of the boar genital organs. Immunohistochemical analysis demonstrated that PON1 was present in testis (specifically in Leydig cells, blood vessels, spermatogonia and elongated spermatids), epididymis (specifically in the cytoplasm of the principal epithelial cells, luminal secretion and in the surrounding smooth muscle) and the lining epithelia of the accessory sexual glands (cytoplasmic location in the prostate and membranous in the seminal vesicle and bulbourethral glands)...
June 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28608609/artificial-insemination-with-frozen-thawed-boar-sperm
#6
REVIEW
Marc Yeste, Joan E Rodríguez-Gil, Sergi Bonet
Artificial insemination with frozen-thawed semen in pigs is not a routine technique; its use is restricted to specific cases, such as preservation of valuable genetic material (germplasm banks), safety strategies in case of natural disasters, long-distance transport of sperm, and in combination with sex-sorting. Cryoinjuries resulting from freeze-thawing protocols are a major concern with regard to the fertilization capacity of the treated sperm, which is lower than that of liquid-stored semen. Here we provide an overview of artificial insemination using cryopreserved sperm, and summarize the factors that influence cryopreservation success before, during, and after freeze-thaw (i...
June 13, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28604785/genome-wide-association-analysis-and-functional-annotation-of-positional-candidate-genes-for-feed-conversion-efficiency-and-growth-rate-in-pigs
#7
Justyna Horodyska, Ruth M Hamill, Patrick F Varley, Henry Reyer, Klaus Wimmers
Feed conversion efficiency is a measure of how well an animal converts feed into live weight and it is typically expressed as feed conversion ratio (FCR). FCR and related traits like growth rate (e.g. days to 110 kg-D110) are of high interest for animal breeders, farmers and society due to implications on animal performance, feeding costs and environmental sustainability. The objective of this study was to identify genomic regions associated with FCR and D110 in pigs. A total of 952 terminal line boars, showing an individual variation in FCR, were genotyped using 60K SNP-Chips...
2017: PloS One
https://www.readbyqxmd.com/read/28601155/boar-genotype-as-a-factor-shaping-age-related-changes-in-semen-parameters-and-reproduction-longevity-simulations
#8
Damian Knecht, Anna Jankowska-Mąkosa, Kamil Duziński
The aim of this study was a detailed analysis of the boar genotypes used in AI stations with an indication of their production capacity, including age and a precise analysis of their culling time and reason. The study included 334 boars: 81 Polish Large White (PLW), 108 Polish Landrace (PL), 49 Pietrain (P), 56 Duroc × Pietrain (D × P) and 40 Hampshire × Pietrain (H × P). Semen volume, spermatozoa concentration, total number of spermatozoa, number of motile spermatozoa, and number of insemination doses were analyzed...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28594953/non-invasive-genetics-outperforms-morphological-methods-in-faecal-dietary-analysis-revealing-wild-boar-as-a-considerable-conservation-concern-for-ground-nesting-birds
#9
Ragne Oja, Egle Soe, Harri Valdmann, Urmas Saarma
Capercaillie (Tetrao urogallus) and other grouse species represent conservation concerns across Europe due to their negative abundance trends. In addition to habitat deterioration, predation is considered a major factor contributing to population declines. While the role of generalist predators on grouse predation is relatively well known, the impact of the omnivorous wild boar has remained elusive. We hypothesize that wild boar is an important predator of ground-nesting birds, but has been neglected as a bird predator because traditional morphological methods underestimate the proportion of birds in wild boar diet...
2017: PloS One
https://www.readbyqxmd.com/read/28583614/effects-of-active-immunization-against-gnrh-versus-surgical-castration-on-hypothalamic-pituitary-function-in-boars
#10
Xingfa Han, Yuqin Zhou, Yu Zeng, Fenfen Sui, Yacheng Liu, Yao Tan, Xiaohan Cao, Xiaogang Du, Fengyan Meng, Xianyin Zeng
The objective was to compare effects of anti-GnRH immunization (immunocastration) versus surgical castration on hypothalamic-pituitary function in boars. Thirty-six boars were randomly divided into three groups (n = 12/group): control, surgically castrated, or immunized against GnRH at 10 wk of age (boostered 8 wk later). Compared to intact boars, immunocastration reduced (P < 0.05) serum concentrations of LH, FSH, testosterone and inhibin B and caused severe testicular atrophy, whereas surgical castration increased (P < 0...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28580140/analysis-of-the-lifetime-and-culling-reasons-for-ai-boars
#11
Damian Knecht, Anna Jankowska-Mąkosa, Kamil Duziński
BACKGROUND: The aim of the study was to analyze the lifetime and culling reasons for boars used in insemination centers (AI centers). METHODS: The data collected from 355 culled boars from 1998 to 2013 included: age at start of semen collection, boar herd life, culling reason, daily gain and lean meat content, and number of ejaculates not meeting sales requirements after dilution. Culling reasons were divided into 7 groups: low semen value (LSV), low or lack of libido (LL), leg problems (LP), infectious diseases (ID), old age (OA), reduced demand for semen from the given boar (RD), and others (OT)...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28576677/impact-of-different-dilution-techniques-on-boar-sperm-quality-and-sperm-distribution-of-the-extended-ejaculate
#12
M Schulze, C Ammon, J Schaefer, A-M Luther, M Jung, D Waberski
The dilution of ejaculates is a fundamental step for the production of liquid-preserved boar semen. For a long time, it has been recommended to add the extender to the ejaculate. The aim of the present study was to first compare the effect of the position ('center' vs. 'wall') where the extender is added to the semen-mixing cylinder (height 32.5cm; diameter 12.7cm) using an automatic dispenser (n=11). In experiment 2 (n=30), we analyzed the two main dilution methods (extender to the semen ('control') vs. 'reverse')...
May 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28576621/chelating-agents-in-combination-with-rosmarinic-acid-for-boar-sperm-freeze-drying
#13
Maite Olaciregui, Victoria Luño, Noelia González, Paula Domingo, Ignacio de Blas, Lydia Gil
The presence of DNA protective agents in the medium is necessary to maintain sperm functionality after freeze-drying procedure. The objective of this study was to investigate the effect of chelating agents, ethylene diaminetetraacetic acid (EDTA) and ethylene glycoltetraacetic acid (EGTA), in combination with rosmarinic acid (RA) on DNA integrity of freeze-dried boar sperm. We also examined the effect of these agents on the in vitro developmental ability of porcine oocytes following sperm injection (ICSI). Heterospermic mix, obtained from ejaculated sperm of three boars, was freeze-dried in two different chelating agents' media: 50mM EDTA or 50mM EGTA, and in these media supplemented with 105μM of rosmarinic acid...
May 30, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28573011/behaviour-of-free-ranging-wild-boar-towards-their-dead-fellows-potential-implications-for-the-transmission-of-african-swine-fever
#14
Carolina Probst, Anja Globig, Bent Knoll, Franz J Conraths, Klaus Depner
The behaviour of free ranging wild boar (Sus scrofa) towards carcasses of their conspecifics potentially infected with African swine fever (ASF) may significantly influence the course of an ASF epidemic. This study aims to better understand the behaviour of wild boar towards their dead fellows. Thirty-two wild boar carcasses on nine study sites in northeast Germany were monitored under field conditions by photo-trapping from October 2015 until October 2016. During this period, a total of 122 160 pictures were taken, thereof 16 111 pictures of wild boar...
May 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28569505/copper-i-complexes-of-a-flexible-bis-phospholane-ligand-route-to-paddle-wheel-and-box-type-macrocycles
#15
Paul Boar, Markus Streitberger, Peter Lönnecke, Evamarie Hey-Hawkins
Two bis-phospholane copper(I) metallamacrocycles were selectively synthesized starting from the same two building blocks, namely, ligand 1 and [Cu(NCCH3)4]BF4. Reaction conditions (ligand:metal (L:M) ratio and dilution) can be tuned to obtain either a paddle-wheel- (2, L:M = 3:2) or box-type complex (3, L:M = 8:4). Their structures were unequivocally determined by X-ray crystallography. The solution (31)P{(1)H} NMR spectrum of complex 2 consists of a broad signal, as is common for such complexes, whereas complex 3 shows splitting of the (31)P{(1)H} NMR signal into a pseudoquartet due to (63,65)Cu-(31)P coupling, a rare occurrence exhibited only by highly symmetrical copper(I) phosphine complexes...
June 1, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28568977/reproductive-performance-and-expression-of-imprinted-genes-in-somatic-cell-cloned-boars
#16
Tatsuo Kawarasaki, Satoko Enya, Masayoshi Otake, Masatoshi Shibata, Satoshi Mikawa
To assess the performance of boars derived by somatic cell cloning, we analyzed various aspects of their reproductive characteristics and the expression of two imprinted genes. Cloned boars (cloned Duroc × Jinhua) were analyzed for birth weight, growth rate, age at first ejaculation, semen characteristics and fertility, in comparison with naturally bred control boars of the same strain. The expression of imprinted genes was analyzed using the microsatellite marker SWC9 for the paternally expressed gene insulin-like growth factor -2 (IGF2) and with single nucleotide polymorphisms (SNPs) for the gene maternally expressed 3 (MEG3)...
June 1, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28557348/the-toxic-mode-of-action-of-cyclic-lipodepsipeptide-fusaricidins-produced-by-paenibacillus-polymyxa-toward-mammalian-cells
#17
Raimo Mikkola, Maria A Andersson, Pavel Grigoriev, Mari Heinonen, Mirja S Salkinoja-Salonen
AIMS: The toxic strains of Paenibacillus polymyxa were isolated from buildings connected to ill health symptoms. Our aim was to identify the toxic compounds of Paenibacillus polymyxa and describe their toxic actions. METHODS AND RESULTS: The toxins of Paenibacillus polymyxa were purified and analysed by HPLC and mass spectrometry. Toxic fusaricidins A and B, and LI-F05a with mass ions at m/z 883.7, 897.6, and 897.6, respectively, were found. Cytotoxicity of purified fusaricidins A and B were measured using boar sperm, porcine tubular kidney epithelial and murine fibroblast cells...
May 29, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28551745/diversity-and-activity-patterns-of-sympatric-animals-among-four-types-of-forest-habitat-in-guanyinshan-nature-reserve-in-the-qinling-mountains-china
#18
Xuehua Liu, Pengfeng Wu, Xiaoming Shao, Melissa Songer, Qiong Cai, Xiangbo He, Yun Zhu
Environmental heterogeneity contributes to various habitats and may influence the diversity and activity patterns of wildlife among habitats. We used camera traps to assess wildlife habitat use in Guanyinshan Nature Reserve from 2009 to 2012. We focused on four types of habitat including open areas with gentle slope (<15°) (Type1), low elevation areas (about 1500-1700 m) with high bamboo coverage (Type2), high elevation areas (about 2100-2300 m) with high canopy coverage (Type3), and wildlife migration passages (Type4)...
May 27, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28550945/pre-birth-sense-of-smell-in-the-wild-boar-the-ontogeny-of-the-olfactory-mucosa
#19
Domenico Fulgione, Martina Trapanese, Maria Buglione, Daniela Rippa, Gianluca Polese, Viviana Maresca, Valeria Maselli
Animals recognize their surrounding environments through the sense of smell by detecting thousands of chemical odorants. Wild boars (Sus scrofa) completely depend on their ability to recognize chemical odorants: to detect food, during scavenging and searching partners, during breeding periods and to avoid potential predators. Wild piglets must be prepared for the chemical universe that they will enter after birth, and they show intense neuronal activity in the olfactory mucosa. With this in mind, we investigated the morpho-functional embryonic development of the olfactory mucosa in the wild boar (in five stages before birth)...
May 17, 2017: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/28539617/characterization-of-porcine-simple-sequence-repeat-variation-on-a-population-scale-with-genome-resequencing-data
#20
Congcong Liu, Yan Liu, Xinyi Zhang, Xuewen Xu, Shuhong Zhao
Simple sequence repeats (SSRs) are used as polymorphic molecular markers in many species. They contribute very important functional variations in a range of complex traits; however, little is known about the variation of most SSRs in pig populations. Here, using genome resequencing data, we identified ~0.63 million polymorphic SSR loci from more than 100 individuals. Through intensive analysis of this dataset, we found that the SSR motif composition, motif length, total length of alleles and distribution of alleles all contribute to SSR variability...
May 24, 2017: Scientific Reports
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