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https://www.readbyqxmd.com/read/29144025/detection-of-atypical-porcine-pestivirus-in-semen-from-commercial-boar-studs-in-the-united-states
#1
I R H Gatto, P H Arruda, C A Visek, J G Victoria, A R Patterson, A C Krull, K J Schwartz, L G de Oliveira, B L Arruda
Atypical porcine pestivirus (APPV) has recently been identified as a cause of congenital tremor (CT) in pigs and has been detected in semen and preputial swabs from boars that were known to be clinically affected with CT. Accordingly, the objectives of this study were to 1) detect the presence of APPV in semen, preputial fluids and preputial swabs from adult boars by quantitative reverse transcription PCR (qRT-PCR) and 2) genetically characterize a subset of positive samples to better understand the ecology of APPV in commercial boar studs and the potential risk of transmission of APPV via semen...
November 16, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29143706/potential-sensitivity-of-pork-production-situations-aiming-at-high-quality-products-to-the-use-of-entire-male-pigs-as-an-alternative-to-surgical-castrates
#2
M Bonneau, M Čandek-Potokar, M Škrlep, M Font-I-Furnols, M Aluwé, L Fontanesi
The perspective of a possible ban on surgical castration of male pigs in the EU is a real challenge for pork production systems aiming at (very) high-quality products. Information was collected from a total of 272 situations in 16 European countries, including 170 situations related to EU protected designations (Database of Origin & Registration (DOOR) database) and 102 other situations related to high-quality products or differentiated production systems, in order to evaluate their potential sensitivity to the use of entire male pigs along four dimensions: BT_Inc, likelihood of increased levels of boar taint compounds compared with conventional production of entire males; BT_Per, extent to which (some of) the associated pork product(s) are susceptible to perception of boar taint by consumers; FatQQ, likelihood that the quality of (some of) the related products is decreased due to the lower fat quantity and quality in entire males; Manag, increased likelihood of animal management and welfare problems compared with conventional production of entire males...
November 16, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29141628/gene-expression-analyses-of-the-small-intestine-of-pigs-in-the-ex-evacuation-zone-of-the-fukushima-daiichi-nuclear-power-plant
#3
Motoko Morimoto, Ayaka Kato, Jin Kobayashi, Kei Okuda, Yoshikazu Kuwahara, Yasushi Kino, Yasuyuki Abe, Tsutomu Sekine, Tomokazu Fukuda, Emiko Isogai, Manabu Fukumoto
BACKGROUND: After the accident at the Fukushima Daiichi Nuclear Power Plant, radioactive contaminants were released over a widespread area. Monitoring the biological effects of radiation exposure in animals in the ex-evacuation zone should be continued to understand the health effects of radiation exposure in humans. The present study aimed to clarify the effects of radiation by investigating whether there is any alteration in the morphology and gene expressions of immune molecules in the intestine of pigs and inobuta (wild boar and domestic pig hybrid) in the ex-evacuation zone in 2012...
November 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29135356/antibody-prevalence-to-influenza-type-a-in-wild-boar-of-northern-ukraine
#4
Ganna Kovalenko, Alona Molozhanova, Ihor Halka, Serhiy Nychyk
A preliminary serological survey was carried out to assess the likelihood of influenza A (IA) infection in wild boar and begin to characterize the role of wild boar in the epidemiology of the IA virus (IAV). Sera collected from 120 wild boar that were hunted in 2014 were tested. To detect antibodies to IA, a blocking the enzyme-linked immunosorbent assay (ELISA) was used. Thirty boar were collected from each of four oblasts in the north central and northwestern regions of Ukraine. Antibodies against IAV were detected in 27 samples (22...
November 14, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29134088/on-the-lack-of-a-universal-pattern-associated-with-mammalian-domestication-differences-in-skull-growth-trajectories-across-phylogeny
#5
Marcelo R Sánchez-Villagra, Valentina Segura, Madeleine Geiger, Laura Heck, Kristof Veitschegger, David Flores
As shown in a taxonomically broad study, domestication modifies postnatal growth. Skull shape across 1128 individuals was characterized by 14 linear measurements, comparing 13 pairs of wild versus domesticated forms. Among wild forms, the boar, the rabbit and the wolf have the highest proportion of allometric growth, explaining in part the great morphological diversity of the domesticated forms of these species. Wild forms exhibit more isometric growth than their domesticated counterparts. Multivariate comparisons show that dogs and llamas exhibit the greatest amount of differences in trajectories with their wild counterparts...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29128698/seminal-plasma-antioxidants-are-directly-involved-in-boar-sperm-cryotolerance
#6
Junwei Li, Isabel Barranco, Asta Tvarijonaviciute, Manuel F Molina, Emilio A Martinez, Heriberto Rodriguez-Martinez, Inmaculada Parrilla, Jordi Roca
Boar ejaculates are ejected in fractions with a specific composition in terms of sperm numbers and seminal plasma (SP), which is reflected in the varying sperm cryotolerance observed among different fractions. As boar sperm are particularly sensitive to oxidative stress, this study evaluated the role of SP antioxidants in the observed differences in sperm cryotolerance among ejaculate fractions. Ten ejaculates from five boars were manually collected in fractions: the first 10 mL of the sperm-rich fraction (SRF), the rest of the SRF and the post-SRF...
October 31, 2017: Theriogenology
https://www.readbyqxmd.com/read/29121524/eating-quality-traits-of-shoulder-roast-and-stir-fry-cuts-outperformed-loin-and-silverside-cuts-sourced-from-entire-and-immunocastrated-male-pigs
#7
H A Channon, D N D'Souza, F R Dunshea
This study investigated the effects of ageing period (2 or 7days), endpoint temperature (70 or 75°C), cut type (loin (M. longissimus thoracis et lumborum), silverside (M. biceps femoris), blade (M. triceps brachii) and chuck tender (M. supraspinatus)), cooking method (roast and stir fry (all cuts) and steak (loin only)) on eating quality attributes of pork from entire and immunocastrated male pigs. Higher intramuscular fat levels were found in all cuts from immunocastrated males compared with entire males...
October 31, 2017: Meat Science
https://www.readbyqxmd.com/read/29119857/mir-638-inhibits-immature-sertoli-cell-growth-by-indirectly-inactivating-pi3k-akt-pathway-via-spag1-gene
#8
Pandi Hu, Kaifeng Guan, Yue Feng, Changping Ma, Huibin Song, Yang Li, Xuanyan Xia, Jialian Li, Fenge Li
Numerous studies have demonstrated that microRNAs (miRNAs) play important roles in cell growth, apoptosis and spermatogenesis. Our previous study showed that miR-638 was differentially expressed in sexually immature and mature testes of Large White boars. Here we reported that sperm-associated antigen 1 (SPAG1) was a direct target gene of miR-638. Moreover, miR-638 inhibited cell proliferation and cell cycle, and promoted apoptosis of porcine immature Sertoli cells. Key genes including phosphorylated phosphatidylinositide 3-kinases (p-PI3K) and phosphorylated serine/ threonine kinase (p-AKT) in PI3K/AKT pathway as well as cell cycle factors including c-MYC, cyclin-D1 (CCND1), cyclin-E1 (CCNE1) and cyclin-dependent kinase 4 (CDK4) were all significantly down-regulated after overexpression of miR-638 or RNAi of SPAG1...
November 9, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29117209/persistence-of-hepatitis-e-virus-in-the-liver-of-non-viremic-naturally-infected-wild-boar
#9
María A Risalde, Antonio Rivero-Juárez, Fernando Romero-Palomo, Mario Frías, Pedro López-López, David Cano-Terriza, Ignacio García-Bocanegra, Saúl Jiménez-Ruíz, Ángela Camacho, Isabel Machuca, José C Gomez-Villamandos, Antonio Rivero
Hepatitis E virus (HEV) is an emerging zoonotic pathogen with pigs and wild boar serving as reservoirs for human infection through direct contact with infected animals or the consumption of raw or undercooked pork products. The liver is considered the main target site of HEV replication in swine and an important organ in the pathogenesis of the disease. The aim of this study was to characterize the target liver cells for HEV entry in naturally infected wild boar and to evaluate the type and severity of the pathological changes in order to reach a better understanding of the hepatic pathogenic mechanisms involved in hepatitis E...
2017: PloS One
https://www.readbyqxmd.com/read/29113727/effect-of-single-layer-centrifugation-porcicoll-70-80-and-90-or-supplementation-with-reduced-glutathione-seminal-plasma-and-bovine-serum-albumin-on-frozen-thawed-boar-sperm
#10
I Crespo-Félez, A Castañeda-Sampedro, D I Sánchez, E Fernández-Alegre, M Álvarez-Rodríguez, J C Domínguez, J M Morrell, F Martínez-Pastor
Selecting the optimal sperm population is essential for success with reproductive techniques. Porcicoll (formerly Androcoll-P) is a colloid formulation for selection of high-quality boar spermatozoa by single layer centrifugation (SLC). To date, most studies have been carried out with fresh semen and large volumes. We carried out 2 experiments to test the use of Porcicoll for thawed boar semen in small volumes. In Experiment 1, cryopreserved semen doses were thawed, split in 200-μL aliquots and layered on 1mL of Porcicoll 70%, 80% or 90%, or buffer without colloid...
November 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29113556/phospholipase-d-family-member-6-pld6-is-a-surface-marker-for-enrichment-of-undifferentiated-spermatogonia-in-the-pre-pubertal-boars
#11
Pengfei Zhang, Yuwei Qin, Yi Zheng, Wenxian Zeng
Spermatogonial stem cells (SSCs) have a functional ability to maintain self-renewal and sustain production of spermatozoa throughout the reproductive lifespan of a male. Studies on SSCs can thus better understandings of spermatogenesis and unravel the mechanisms for self-renewal and differentiation of male germline stem cells. However, the rarity of SSCs in the testis and the lack of reliable surface markers obstruct the related study and further application of SSCs. This is especially the case in livestock animals...
November 7, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29108065/estimation-of-the-effects-of-selection-on-french-large-white-sow-and-piglet-performance-during-the-suckling-period
#12
P Silalahi, T Tribout, Y Billon, J Gogué, J P Bidanel
The effects of 21 yr of selection were estimated for sow and piglet performance during the suckling period in a French Large White (LW) pig population using frozen semen. Two experimental groups (EXP = L77 and L98) were produced by inseminating LW sows with either stored frozen semen from 17 LW boars born in 1977 (EXP = L77) or with fresh semen from 23 LW boars born in 1998 (EXP = L98). Seventy-four L77 and 89 L98 randomly chosen females were mated to 15 L77 and 15 L98, respectively, randomly chosen boars for 6 successive parities...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29108030/genetic-parameters-for-semen-quality-and-quantity-traits-in-five-pig-lines
#13
D B D Marques, M S Lopes, M L W J Broekhuijse, S E F Guimarães, E F Knol, J W M Bastiaansen, F F Silva, P S Lopes
We aimed to estimate genetic parameters for semen quality and quantity traits as well as for within-boar variation of these traits to evaluate their inclusion in breeding goals. Genetic parameters were estimated within line using a multiple-trait (4 × 4) repeatability animal model fitted for 5 pig lines, considering 4 semen traits: sperm motility (MOT), sperm progressive motility (PROMOT), log-transformed number of sperm cells per ejaculate (lnN), and total morphological abnormalities (ABN). The within-boar variation of these traits was analyzed based on a multiple-trait (2 × 2) approach for SD and average (AVG) and a single-trait analysis for CV...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29107386/impact-of-holding-and-equilibration-time-on-post-thaw-quality-of-shipped-boar-semen
#14
J Schäfer, D Waberski, M Jung, M Schulze
Cryopreservation of boar semen is of growing interest for breeding companies. Overnight-shipping of pre-diluted ejaculates to specialized laboratories offers a practicable method, but requires fine-tuned protocols. In this study, the impact of holding post shipping at 17°C for 2 or 24h (n=10 samples) and of equilibration in lactose-egg yolk extender without glycerol at 5°C for 2, 4, 24 or 48h (n=11 samples) before freezing was investigated. Sperm-rich fractions of ejaculates from 21 mature Pietrain boars were collected at a single boar stud...
October 27, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29103700/mlva-16-typing-of-brucella-suis-biovar-2-strains-circulating-in-europe
#15
A C Ferreira, M I Corrêa de Sá, R Dias, R Tenreiro
Swine brucellosis due to Brucella suis biovar 2 is an emerging disease in Europe, associated with increase of extensive swine farms and high density of infected wild boars. Since knowledge of predominant circulating strains is a prerequisite for any epidemiological study, accurate molecular typing procedures were applied to a collection of 176 B. suis isolates. By using suis-ladder multiplex PCR and PCR-RFLP analysis of omp2a, omp2b and omp31 genes, five haplotypes were identified among 160 biovar 2 isolates, with haplotypes 2d and 2e restricted to Portugal and Spain and haplotypes 2a, 2b and 2c widespread in Europe (except Portugal)...
October 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29096150/comparison-of-different-methodologies-for-the-90-sr-determination-in-environmental-samples
#16
Željko Grahek, Silvia Dulanská, Gorana Karanović, Ivana Coha, Ivana Tucaković, Marijana Nodilo, Ľubomír Mátel
The paper describes different isolation/separation and detection procedures for (90)Sr determination in the environmental samples which are routinely used in Laboratories A and B. In this context, four different methods for strontium isolation and two methods for detection were tested and compared by (90)Sr determination in proficiency test samples (water, soil, vegetation) and animal bone samples. The chromatographic isolation of Sr on Sr resin, AnaLig(®)Sr01 resin gel, strong base anion exchange resins in nitrate form and combination of strong base anion exchange and Sr resin were used for the examination of the impact of sample matrix constituents on efficiency of strontium isolation (chemical yield), while Cherenkov counting of (90)Y and counting of (90)Sr((90)Y) on proportional counter were used for the quantitative (90)Sr determination...
October 28, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/29090093/effects-of-pituitary-specific-overexpression-of-fsh%C3%AE-%C3%AE-on-reproductive-traits-in-transgenic-boars
#17
Wenting Li, Yujun Quan, Mengmeng Zhang, Kejun Wang, Muzhen Zhu, Ye Chen, Qiuyan Li, Keliang Wu
BACKGROUND: Follicle-stimulating hormone (FSH) is a gonadotropin synthesized and secreted by the pituitary gland. FSH stimulates follicle development and maturation in females. It also plays an important role in spermatogenesis in males, including humans and mice. However, the effects of FSH on male pigs are largely unknown. In this study, we generated transgenic pigs to investigate the effects of FSHα/β overexpression on reproductive traits in boars. RESULTS: After five transgenic F0 founders were crossed with wide-type pigs, 193 F1 animals were obtained...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29082338/a-simple-confocal-microscopy-based-method-for-assessing-sperm-movement
#18
Sung Woo Kim, Min Su Ki, Chan-Lan Kim, In-Sul Hwang, Ik Soo Jeon
In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29081591/seroprevalence-of-mycoplasma-suis-infection-in-pigs-in-eastern-china-as-estimated-by-a-blocking-enzyme-linked-immunosorbent-assay
#19
Liang Zhongyang, Zhang Jiansong, Shen Yijuan, Xia Yuting, Li Yufeng, Xu Jiarong
Porcine infectious anemia caused by Mycoplasma suis is a global disease and results in serious economic losses. To determine the prevalence of M. suis infection in eastern China, a cross-sectional serologic study was conducted with 3458 porcine serum samples randomly obtained from January 2014 to August 2016. The samples were tested with a blocking enzyme-linked immunosorbent assay developed in our laboratory. The seroprevalence was 33.3% in the complete sample set and was 25.9%, 37.8%, and 37.8% in 2014, 2015, and 2016, respectively...
October 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/29081440/cluster-analysis-reveals-seasonal-variation-of-sperm-subpopulations-in-extended-boar-semen
#20
Iulian Ibănescu, Claus Leiding, Heinrich Bollwein
This study aimed to identify motile sperm subpopulations in extended boar semen and to observe the presumptive seasonal variation in their distribution. Data from 4837 boar ejaculates collected over a two-year period were analyzed in terms of kinematic parameters by Computer Assisted Sperm Analysis (CASA). Individual sperm data were used to determine subgroups of motile sperm within the ejaculates using cluster analysis. Four motile sperm subpopulations (SP) were identified, with distinct movement patterns: SP1 sperm with high velocity and high linearity; SP2 sperm with high velocity but low linearity; SP3 sperm with low velocity but high linearity; and SP4 sperm with low velocity and low linearity...
October 28, 2017: Journal of Reproduction and Development
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