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https://www.readbyqxmd.com/read/28919276/-focus-on-achalasia
#1
Marie-Anne Guillaumot, Maximilien Barret, Sarah Leblanc, Mahaut Leconte, Bertrand Dousset, Ammar Oudjit, Frédéric Prat, Stanislas Chaussade
The pathophysiology of achalasia is largely unknown, and involves the destruction of ganglion cell in the esophageal myenteric plexus. High-resolution esophageal manometry is the key investigation. Endoscopic pneumodilatation and laparoscopic Heller myotomy have comparable short-term success rates, around 90%. The main complication after pneumodilatation is esophageal perforation, occurring in about 1% of cases. Peroral endoscopic myotomy is a promising treatment modality, however with frequent post-procedural gastroesophageal reflux...
September 14, 2017: La Presse Médicale
https://www.readbyqxmd.com/read/28918872/-management-of-ablative-therapies-in-prostate-cancer
#2
E Barret, R Sanchez-Salas, M Galiano, N Cathala, A Mombet, D Prapotnich, F Rozet, A Gangi, H Lang, X Cathelineau
OBJECTIVES: To describe the specific modalities of ablative therapies management in prostate cancer. MATERIALS AND METHODS: A review of the scientific literature was performed in Medline database (http://www.ncbi.nlm.nih.gov) and Embase (http://www.embase.com) using different associations of keywords. Publications obtained were selected based on methodology, language and relevance. After selection, 61 articles were analysed. RESULTS: Development of innovations such as ablative therapies in prostate cancer induces specific modalities in their management, during pre-, per- and post-procedure...
September 14, 2017: Progrès en Urologie
https://www.readbyqxmd.com/read/28918871/-indications-and-limits-of-ablative-therapies-in-prostate-cancer
#3
R Sanchez-Salas, E Barret, R Renard-Penna, J-M Cosset, P Dell'Oglio, J Battista, A Gangi, H Lang, X Cathelineau
OBJECTIVE: To perform a state of the art about indications and limits of ablative therapies for localized prostate cancer. METHODS: A review of the scientific literature was performed in Medline database (http://www.ncbi.nlm.nih.gov) and Embase (http://www.embase.com) using different associations of keywords. Publications obtained were selected based on methodology, language and relevance. After selection, 107 articles were analysed. RESULTS: The objective to combine reduction of side effects and oncological control has induced recent development of several ablative therapies...
September 14, 2017: Progrès en Urologie
https://www.readbyqxmd.com/read/28913799/biolistic-transformation-of-wheat
#4
Caroline Tassy, Pierre Barret
The wheat genome encodes some 100,000 genes. To understand how the expression of these genes is regulated it will be necessary to carry out many genetic transformation experiments. Robust protocols that allow scientists to transform a wide range of wheat genotypes are therefore required. In this chapter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28869687/ancestral-acquisitions-gene-flow-and-multiple-evolutionary-trajectories-of-the-type-three-secretion-system-and-effectors-in-xanthomonas-plant-pathogens
#5
Déborah Merda, Martial Briand, Eran Bosis, Céline Rousseau, Perrine Portier, Matthieu Barret, Marie-Agnès Jacques, Marion Fischer-Le Saux
Deciphering the evolutionary history and transmission patterns of virulence determinants is necessary to understand the emergence of novel pathogens. The main virulence determinant of most pathogenic proteobacteria is the type three secretion system (T3SS). The Xanthomonas genus includes bacteria responsible for numerous epidemics in agroecosystems worldwide and represents a major threat to plant health. The main virulence factor of Xanthomonas is the Hrp2 family T3SS, however this system is not conserved in all strains and it has not been previously determined whether the distribution of T3SS in this bacterial genus has resulted from losses or independent acquisitions...
September 4, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28859394/esophageal-achalasia-a-risk-factor-for-carcinoma-a-systematic-review-and-meta-analysis
#6
F Tustumi, W M Bernardo, J R M da Rocha, S Szachnowicz, F C Seguro, E T Bianchi, R A A Sallum, I Cecconello
Achalasia of the cardia is associated with an increased risk of esophageal carcinoma. The real burden of achalasia at the malignancy genesis is still a controversial issue. Therefore, there are no generally accepted recommendations on follow-up evaluation for achalasia patients. This study aims to estimate the risk of esophageal adenocarcinoma and squamous cell carcinoma in achalasia patients. We searched for association between carcinoma and esophageal achalasia in databases up to January 2017 to perform a systematic review and meta-analysis...
October 1, 2017: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28858617/a-circuit-node-that-integrates-convergent-input-from-neuromodulatory-and-social-behavior-promoting-neurons-to-control-aggression-in-drosophila
#7
Kiichi Watanabe, Hui Chiu, Barret D Pfeiffer, Allan M Wong, Eric D Hoopfer, Gerald M Rubin, David J Anderson
Diffuse neuromodulatory systems such as norepinephrine (NE) control brain-wide states such as arousal, but whether they control complex social behaviors more specifically is not clear. Octopamine (OA), the insect homolog of NE, is known to promote both arousal and aggression. We have performed a systematic, unbiased screen to identify OA receptor-expressing neurons (OARNs) that control aggression in Drosophila. Our results uncover a tiny population of male-specific aSP2 neurons that mediate a specific influence of OA on aggression, independent of any effect on arousal...
August 30, 2017: Neuron
https://www.readbyqxmd.com/read/28826226/patterns-of-palliative-care-referral-in-patients-admitted-with-heart-failure-requiring-mechanical-ventilation
#8
Katie J Wiskar, Leo Anthony Celi, Robert C McDermid, Keith R Walley, James A Russell, John H Boyd, Barret Rush
BACKGROUND: Palliative care is recommended for advanced heart failure (HF) by several major societies, though prior studies indicate that it is underutilized. AIM: To investigate patterns of palliative care referral for patients admitted with HF exacerbations, as well as to examine patient and hospital factors associated with different rates of palliative care referral. DESIGN: Retrospective nationwide cohort analysis utilizing the National Inpatient Sample from 2006 to 2012...
January 1, 2017: American Journal of Hospice & Palliative Care
https://www.readbyqxmd.com/read/28780054/pseudoachalasia
#9
Maximilien Barret, Alexandre Rouquette, Pierre-Philippe Massault, Caroline Prieux, Bertrand Brieau, Romain Coriat, Stanislas Chaussade
No abstract text is available yet for this article.
August 2, 2017: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/28754326/diagnosis-of-acute-overt-gastrointestinal-bleeding-with-ct-angiography-comparison-of-the-diagnostic-performance-of-individual-acquisition-phases
#10
E Shotar, P Soyer, M Barat, R Dautry, M Pocard, V Placé, M Camus, C Eveno, M Barret, A Dohan
PURPOSE: To compare the respective values of arterial phase, portal venous phase and combination of phases using 64-section multidetector computed tomography (MDCT) for diagnosing acute overt gastrointestinal bleeding (AOGIB). PATIENTS AND METHODS: Forty-nine patients with AOGIB were included. There were 30 men and 19 women, with a mean age of 65.4±15.6 (SD) years [range, 34-91years]. Two observers reviewed MDCT examinations in consensus for presence of active bleeding, location of bleeding site and nature of causative lesion...
July 25, 2017: Diagnostic and Interventional Imaging
https://www.readbyqxmd.com/read/28749333/a-cluster-of-invasive-meningococcal-disease-imd-caused-by-neisseria-meningitidis-serogroup-w-among-university-students-france-february-to-may-2017
#11
Clément Bassi, Muhamed-Kheir Taha, Christian Merle, Eva Hong, Daniel Lévy-Bruhl, Anne-Sophie Barret, Ibrahim Mounchetrou Njoya
Between February and May 2017, two cases of invasive meningococcal disease caused by a new, rapidly expanding serogroup W meningococci variant were reported among students of an international university in Paris. Bacteriological investigations showed that isolates shared identical genotypic formula (W:P1.5,2:F1-1:cc11) and belonged to the South American/UK lineage. A vaccination campaign was organised that aimed at preventing new cases linked to potential persistence of the circulation of the bacteria in the students...
July 13, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28741859/inpatient-palliative-care-referral-and-9-month-hospital-readmission-in-patients-with-congestive-heart-failure-a-linked-nationwide-analysis
#12
K Wiskar, L A Celi, K R Walley, C Fruhstorfer, B Rush
OBJECTIVE: End-stage heart failure (HF) is characterized by high symptom burden and frequent hospitalization. Palliative care (PC) is recommended for advanced HF, and there is some evidence in other diseases that this may reduce readmission rates. We attempted examine the association of an inpatient PC visit on hospital readmission for patients admitted with HF. METHODS: Retrospective linked nationwide analysis from 2013 with 9-month follow-up for all hospital readmissions for patients admitted with HF exacerbations using the Nationwide Readmission Database (NRD)...
July 25, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/28727387/focal-cryotherapy-step-by-step-technique-description
#13
Cristina Redondo, Victor Srougi, José Batista da Costa, Mohammed Baghdad, Guillermo Velilla, Igor Nunes-Silva, Sebastien Bergerat, Silvia Garcia-Barreras, François Rozet, Alexandre Ingels, Marc Galiano, Rafael Sanchez-Salas, Eric Barret, Xavier Cathelineau
INTRODUCTION AND OBJECTIVE: Focal cryotherapy emerged as an efficient option to treat favorable and localized prostate cancer (PCa). The purpose of this video is to describe the procedure step by step. MATERIALS AND METHODS: We present the case of a 68 year-old man with localized PCa in the anterior aspect of the prostate. RESULTS: The procedure is performed under general anesthesia, with the patient in lithotomy position. Briefly, the equipament utilized includes the cryotherapy console coupled with an ultrasound system, argon and helium gas bottles, cryoprobes, temperature probes and an urethral warming catheter...
July 20, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28711280/unsupervised-extraction-of-stable-expression-signatures-from-public-compendia-with-an-ensemble-of-neural-networks
#14
Jie Tan, Georgia Doing, Kimberley A Lewis, Courtney E Price, Kathleen M Chen, Kyle C Cady, Barret Perchuk, Michael T Laub, Deborah A Hogan, Casey S Greene
Cross-experiment comparisons in public data compendia are challenged by unmatched conditions and technical noise. The ADAGE method, which performs unsupervised integration with denoising autoencoder neural networks, can identify biological patterns, but because ADAGE models, like many neural networks, are over-parameterized, different ADAGE models perform equally well. To enhance model robustness and better build signatures consistent with biological pathways, we developed an ensemble ADAGE (eADAGE) that integrated stable signatures across models...
July 26, 2017: Cell Systems
https://www.readbyqxmd.com/read/28691186/per-oral-endoscopic-myotomy-poem-as-a-salvation-technique-post-heller-an-international-experience
#15
Amy Tyberg, Reem Z Sharaiha, Pietro Familiari, Guido Costamagna, Fernando Casas, Nikhil A Kumta, Maximilien Barret, Amit P Desai, Felice Schnoll-Sussman, Payal Saxena, Guadalupe Martínez, Felipe Zamarripa, Monica Gaidhane, Helga Bertani, Peter V Draganov, Valerio Balassone, Ahmed Sharata, Kevin Reavis, Lee Swanstrom, Martina Invernizzi, Stefan Seewald, Hitomi Minami, Haruhiro Inoue, Michel Kahaleh
BACKGROUND: The treatment for achalasia has traditionally been Heller myotomy (HM). Despite its excellent efficacy rate, a number of patients remain symptomatic post-procedure. Limited data exists as to the best management for recurrence of symptoms post HM. We present an international, multicenter experience evaluating the efficacy and safety of post-HM per-oral endoscopic myotomy (POEM). METHODS: Patients who underwent a POEM post-HM from 13 centers from January 2012 to January 2017 were included as part of a prospective registry...
July 10, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/28667403/molecular-and-fish-analyses-of-a-53-kbp-intact-dna-fragment-inserted-by-biolistics-in-wheat-triticum-aestivum-l-genome
#16
A Partier, G Gay, C Tassy, M Beckert, C Feuillet, P Barret
A large, 53-kbp, intact DNA fragment was inserted into the wheat ( Triticum aestivum L.) genome. FISH analyses of individual transgenic events revealed multiple insertions of intact fragments. Transferring large intact DNA fragments containing clusters of resistance genes or complete metabolic pathways into the wheat genome remains a challenge. In a previous work, we showed that the use of dephosphorylated cassettes for wheat transformation enabled the production of simple integration patterns. Here, we used the same technology to produce a cassette containing a 44-kb Arabidopsis thaliana BAC, flanked by one selection gene and one reporter gene...
June 30, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28660622/palliative-care-access-for-hospitalized-patients-with-end-stage-liver-disease-across-the-united-states
#17
Barret Rush, Keith R Walley, Leo Anthony Celi, Neil Rajoriya, Mayur Brahmania
OBJECTIVE: Patients with end-stage liver disease (ESLD) often have a high symptom burden. Historically, palliative care (PC) services have been underutilized in this population. We investigated the use of PC services in patients with ESLD hospitalized across the United States. METHODS: We utilized the Nationwide Inpatient Sample (NIS) to conduct a retrospective nationwide cohort analysis. All patients >18 years of age admitted with ESLD, defined as those with at least two liver decompensation events, were included in the analysis...
June 29, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28655462/evaluation-of-the-antibiotics-effects-on-the-physical-and-chemical-parameters-during-the-co-composting-of-sewage-sludge-with-palm-wastes-in-a-bioreactor
#18
Amine Ezzariai, Maialen Barret, Georges Merlina, Eric Pinelli, Mohamed Hafidi
The objective of this study was to investigate thermal and physicochemical parameters of sewage sludge-palm waste mixtures contaminated by different families of antibiotics (tetracyclines, macrolides and fluoroquinolones) during co-composting. Sludge was spiked with chlortetracycline (CTC), oxytetracycline (OTC), roxithromycin (RXY), enrofloxacin (ENR) and ciprofloxacin (CIP). Antibiotics were spiked at a low level, medium level, high level and a control without antibiotics was conducted. The results showed that the organic matter degradation was delayed and the carbon/nitrogen (C/N) ratio was affected by an increase of the antibiotics concentration...
June 24, 2017: Waste Management
https://www.readbyqxmd.com/read/28648486/focal-brachytherapy-for-localized-prostate-cancer-urinary-toxicity-depends-on-tumor-location
#19
Victor Srougi, Eric Barret, Igor Nunes-Silva, Mohammed Baghdadi, Silvia Garcia-Barreras, Noelle Pierrat, Francois Rozet, Marc Galiano, Rafael Sanchez-Salas, Xavier Cathelineau, Jean-Marc Cosset
PURPOSE: To evaluate whether patients with prostate cancer have worse functional urinary recovery with focal brachytherapy (FBT) at the base versus the apex of the prostate. METHODS AND MATERIALS: The functional outcomes of patients treated with FBT at the base of the prostate were compared with those of patients treated with FBT at the apex. Urinary symptoms, continence, and erectile dysfunction were measured using the International Prostate Symptom Score (IPSS), International Continence Score (ICS), and International Index of Erectile Function (IIEF-5) questionnaires, respectively, at baseline and at 6, 12, and 24 months after treatment...
June 22, 2017: Brachytherapy
https://www.readbyqxmd.com/read/28644320/in-reply
#20
Barret Rush, Pawel Martinka, Robert C McDermid, John H Boyd, Leo Anthony Celi
No abstract text is available yet for this article.
July 2017: Obstetrics and Gynecology
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