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https://www.readbyqxmd.com/read/27926935/serological-and-virological-evidence-of-crimean-congo-haemorrhagic-fever-virus-circulation-in-the-human-population-of-borno-state-northeastern-nigeria
#1
David N Bukbuk, Stuart D Dowall, Kuiama Lewandowski, Andrew Bosworth, Saka S Baba, Anitha Varghese, Robert J Watson, Andrew Bell, Barry Atkinson, Roger Hewson
BACKGROUND: Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria. PRINCIPAL FINDINGS: Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27924816/an-analysis-of-human-exposure-to-trace-elements-from-deliberate-soil-ingestion-and-associated-health-risks
#2
Veronica M Ngole-Jeme, Georges-Ive E Ekosse, Sandile P Songca
Fifty-seven samples of soils commonly ingested in South Africa, Swaziland, Democratic Republic of Congo (DRC), and Togo were analyzed for the concentrations of arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), lead (Pb), manganese (Mn), nickel (Ni), and zinc (Zn) and their bioaccessibility in the human gastrointestinal tract. Bioaccessibility values were used to calculate daily intake, and hazard quotient of each trace element, and chronic hazard index (CHI) of each sample. Carcinogenic risk associated with As and Ni exposure were also calculated...
December 7, 2016: Journal of Exposure Science & Environmental Epidemiology
https://www.readbyqxmd.com/read/27920392/knowledge-attitudes-and-practices-of-health-care-workers-about-viral-hepatitis-b-and-c-in-south-kivu
#3
Tony Akilimali Shindano, Esto Bahizire, René Fiasse, Yves Horsmans
Health-care workers (HCWs) are at risk of infections associated with accidental exposure to blood, including viral hepatitis B (HBV) and C (HCV). A survey using a questionnaire was conducted on 250 HCW in Bukavu, an eastern town of the Democratic Republic of Congo, to analyze their attitude and knowledge about these two viruses. A response rate of 86.8% (217/250) was obtained. The mean age of respondents was 39.6 ± 9.8 years, in majority from paramedical staff (66.4%) and with more than 5 years of professional experience (60...
December 5, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27912123/synthesis-of-hierarchical-porous-zinc-oxide-zno-microspheres-with-highly-efficient-adsorption-of-congo-red
#4
Chunsheng Lei, Meng Pi, Chuanjia Jiang, Bei Cheng, Jiaguo Yu
Hierarchical porous zinc oxide (ZnO) was successfully synthesized via a facile hydrothermal method followed by calcination, and characterized by X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, nitrogen adsorption-desorption, and Fourier transform infrared spectroscopy analyses. The as-prepared porous ZnO exhibits microsphere morphologies with diameters of 6-8μm, which are assembled from two-dimensional nanosheets. The as-prepared hierarchical porous ZnO microspheres possessed high specific surface areas (57m(2)/g), and were evaluated for adsorption of Congo red (CR) in aqueous solution...
November 15, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27911894/head-lice-of-pygmies-reveal-the-presence-of-relapsing-fever-borreliae-in-the-republic-of-congo
#5
Nadia Amanzougaghene, Jean Akiana, Géor Mongo Ndombe, Bernard Davoust, Nardiouf Sjelin Nsana, Henri-Joseph Parra, Florence Fenollar, Didier Raoult, Oleg Mediannikov
BACKGROUND: Head lice, Pediculus humanus capitis, occur in four divergent mitochondrial clades (A, B, C and D), each having particular geographical distributions. Recent studies suggest that head lice, as is the case of body lice, can act as a vector for louse-borne diseases. Therefore, understanding the genetic diversity of lice worldwide is of critical importance to our understanding of the risk of louse-borne diseases. METHODOLOGY/PRINCIPAL FINDINGS: Here, we report the results of the first molecular screening of pygmies' head lice in the Republic of Congo for seven pathogens and an analysis of lice mitochondrial clades...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27906982/using-community-level-prevalence-of-loa-loa-infection-to-predict-the-proportion-of-highly-infected-individuals-statistical-modelling-to-support-lymphatic-filariasis-and-onchocerciasis-elimination-programs
#6
Daniela K Schlüter, Martial L Ndeffo-Mbah, Innocent Takougang, Tony Ukety, Samuel Wanji, Alison P Galvani, Peter J Diggle
Lymphatic Filariasis and Onchocerciasis (river blindness) constitute pressing public health issues in tropical regions. Global elimination programs, involving mass drug administration (MDA), have been launched by the World Health Organisation. Although the drugs used are generally well tolerated, individuals who are highly co-infected with Loa loa are at risk of experiencing serious adverse events. Highly infected individuals are more likely to be found in communities with high prevalence. An understanding of the relationship between individual infection and population-level prevalence can therefore inform decisions on whether MDA can be safely administered in an endemic community...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27905221/antioxidant-potentiality-of-three-herbal-teas-consumed-in-bandundu-rural-areas-of-congo
#7
Paulin Mutwale Kapepula, Patricia Mbombo Mungitshi, Thierry Franck, Ange Mouithys-Mickalad, Dieudonné Mumba Ngoyi, Pascal Dibungi T Kalenda, Nadege Kabamba Ngombe, Didier Serteyn, Monique Tits, Michel Frédérich, Jean-Jacques Tamfum Muyembe
The aim of this study was to evaluate and compare the cellular antioxidant activities of Lantana montevidensis, Lippia multiflora, and Ocimum gratissimum leaves often consumed as herbal teas in a rural area of Bandundu severely affected by konzo, which is related to oxidative damage. Consequently, dietary supplements with proven antioxidant potentialities could be of real interest to promote in this area. Phytochemical screening by TLC and HPLC-DAD of extracts revealed the presence of verbascoside as a major phenolic compound...
December 1, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27902321/the-serology-of-ebolavirus-a-wider-geographical-range-a-wider-genus-of-viruses-or-a-wider-range-of-virulence
#8
Magdalena Formella, Derek Gatherer
Viruses of the genus Ebolavirus are the causative agents of Ebola virus disease (EVD), of which there have been only 25 recorded outbreaks since the discovery of Zaire and Sudan ebolaviruses in the late 1970s. Until the west African outbreak commencing in late 2013, EVD was confined to an area of central Africa stretching from the coast of Gabon through the Congo river basin and eastward to the Great Lakes. Nevertheless, population serological studies since 1976, most of which were carried out in the first two decades after that date, have suggested a wider distribution and more frequent occurrence across tropical Africa...
October 21, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27897378/subcellular-clustering-of-a-putative-c-di-gmp-dependent-exopolysaccharide-machinery-affecting-macro-colony-architecture-in-bacillus-subtilis
#9
Patricia Bedrunka, Peter L Graumann
The structure of bacterial biofilms is predominantly established through the secretion of extracellular polymeric substances (EPS). We show that Bacillus subtilis contains an operon (ydaJ-N) whose induction leads to increased Congo Red staining of biofilms and strongly altered biofilm architecture, suggesting that it mediates the production of an unknown exopolysaccharide. Supporting this idea, overproduction of YdaJKLMN leads to cell clumping during exponential growth in liquid culture, and also causes colony morphology alterations in wild type cells, as well as in a mutant background lacking the major exopolysaccharide of B...
November 29, 2016: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/27890637/antiplasmodial-activity-of-heinsia-crinita-rubiaceae-and-identification-of-new-iridoids
#10
P Tshisekedi Tshibangu, P Mutwale Kapepula, M J Kabongo Kapinga, A Tujibikila Mukuta, Dibungi T Kalenda, Alembert T Tchinda, Ange A Mouithys-Mickalad, O Jansen, E Cieckiewicz, M Tits, L Angenot, M Frédérich
ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in the Democratic Republic of the Congo (DRC) as a remedy against malaria. Investigations were conducted on crude extracts of leaves, fruits and stem barks in view to validate their use and to determine which plant part possesses the best antiplasmodial properties. MATERIALS AND METHODS: Different plant parts were extracted with methanol, ethanol and dichloromethane...
November 24, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27890528/pathological-concentration-of-zinc-dramatically-accelerates-abnormal-aggregation-of-full-length-human-tau-and-thereby-significantly-increases-tau-toxicity-in-neuronal-cells
#11
Ji-Ying Hu, De-Lin Zhang, Xiao-Ling Liu, Xue-Shou Li, Xiao-Qing Cheng, Jie Chen, Hai-Ning Du, Yi Liang
A pathological hallmark of Alzheimer disease and other tauopathies is the formation of neurofibrillary tangles mainly composed of bundles of fibrils formed by microtubule-associated protein Tau. Here we study the effects of Zn(2+) on abnormal aggregation and cytotoxicity of a pathological mutant ΔK280 of full-length human Tau. As revealed by Congo red binding assays, transmission electron microscopy, immunofluorescence, Western blot, and immunogold electron microscopy, pathological concentration of Zn(2+) dramatically accelerates the fibrillization of ΔK280 both in vitro and in SH-SY5Y neuroblastoma cells...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27890366/in%C3%A2-vitro-biofilm-formation-in-esbl-producing-escherichia-coli-isolates-from-cage-birds
#12
Ebru Sebnem Yılmaz, Nur Ceyhan Güvensen
OBJECTIVE: To determine biofilm and hydrophobicity formation ratios in extended spectrum beta lactamases (ESBL) synthesizing Escherichia coli isolates which were isolated from feces samples of 150 cage bird species randomly taken from pet shops in Hatay province, Turkey. METHODS: In vitro biofilm production of 4 ESBL positive isolates were performed by Congo Red Agar (CRA), Standard Tube (ST) and Microtitre Plate (MP) methods while their hydrophobicity were examined by bacterial adhesion to hydrocarbon (BATH) test...
November 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27888125/initiating-ntd-programs-targeting-schistosomiasis-and-soil-transmitted-helminthiasis-in-two-provinces-of-the-democratic-republic-of-the-congo-establishment-of-baseline-prevalence-for-mass-drug-administration
#13
Achille Kabore, Moudachirou Ibikounle, Jean Jacques Tougoue, Sylvain Mupoyi, Martin Ndombe, Scott Shannon, Eric A Ottesen, Faustin Mukunda, Naomi Awaca
BACKGROUND: Schistosomiasis (SCH) and soil-transmitted helminthiasis (STH) are widely distributed in the Democratic Republic of the Congo (DRC) and constitute a serious public health problem. As recommended by the World Health Organization (WHO), before launching mass chemotherapy to control these diseases, parasitological surveys were conducted in sentinel sites in six health zones (HZs) in Bandundu and Maniema provinces. Baseline prevalence and intensity of infection for SCH and STH were determined to establish the appropriate treatment plan using Praziquantel (PZQ) and Albendazole (ALB)...
November 22, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27886627/facilitating-the-development-of-higher-order-thinking-skills-hots-of-novice-nursing-postgraduates-in-africa
#14
Lizeth Roets, Jeanette Maritz
BACKGROUND: International research in nursing education has shown to be deficient regarding both the quality of research produced and the building of disciplinary capacity. The CHENMA (Collaboration for Higher Education of Nurses and Midwives in Africa) project aimed to strengthen nursing and midwifery expertise in Africa. Sixteen French-speaking students of the Democratic Republic of the Congo (DRC) enrolled for a master's degree in nursing midwifery at a South African university in 2008...
November 12, 2016: Nurse Education Today
https://www.readbyqxmd.com/read/27884064/amyloid-fibril-proteins-and-amyloidosis-chemical-identification-and-clinical-classification-international-society-of-amyloidosis-2016-nomenclature-guidelines
#15
Jean D Sipe, Merrill D Benson, Joel N Buxbaum, Shu-Ichi Ikeda, Giampaolo Merlini, Maria J M Saraiva, Per Westermark
The Nomenclature Committee of the International Society of Amyloidosis (ISA) met during the XVth Symposium of the Society, 3 July-7 July 2016, Uppsala, Sweden, to assess and formulate recommendations for nomenclature for amyloid fibril proteins and the clinical classification of the amyloidoses. An amyloid fibril must exhibit affinity for Congo red and with green, yellow or orange birefringence when the Congo red-stained deposits are viewed with polarized light. While congophilia and birefringence remain the gold standard for demonstration of amyloid deposits, new staining and imaging techniques are proving useful...
December 2016: Amyloid: the International Journal of Experimental and Clinical Investigation
https://www.readbyqxmd.com/read/27880794/phylogeography-of-crimean-congo-hemorrhagic-fever-virus
#16
Alexander N Lukashev, Alexander S Klimentov, Svetlana E Smirnova, Tamara K Dzagurova, Jan Felix Drexler, Anatoly P Gmyl
Crimean Congo hemorrhagic fever virus (CCHFV) is one of the most severe viral zoonozes. It is prevalent throughout Africa, Asia and southern Europe. Limited availability of sequence data has hindered phylogeographic studies. The complete genomic sequence of all three segments of 14 Crimean Congo hemorrhagic fever virus strains isolated from 1958-2000 in Russia, Central Asia and Africa was identified. Each genomic segment was independently subjected to continuous Bayesian phylogeographic analysis. The origin of each genomic segment was traced to Africa about 1,000-5,000 years ago...
2016: PloS One
https://www.readbyqxmd.com/read/27880749/early-diagnosis-of-hiv-infection-in-infants-one-caribbean-and-six-sub-saharan-african-countries-2011-2015
#17
Karidia Diallo, Andrea A Kim, Shirley Lecher, Dennis Ellenberger, R Suzanne Beard, Helen Dale, Mackenzie Hurlston, Molly Rivadeneira, Peter N Fonjungo, Laura N Broyles, Guoqing Zhang, Katrina Sleeman, Shon Nguyen, Steve Jadczak, Nadine Abiola, Raimi Ewetola, Jérémie Muwonga, Franck Fwamba, Christina Mwangi, Mary Naluguza, Charles Kiyaga, Isaac Ssewanyana, Deyde Varough, Domercant Wysler, David Lowrance, Frantz Jean Louis, Olbeg Desinor, Josiane Buteau, Francois Kesner, Vanessa Rouzier, Nat Segaren, Tessa Lewis, Abdoulaye Sarr, Geoffrey Chipungu, Sundeep Gupta, Daniel Singer, Reuben Mwenda, Hilary Kapoteza, Zawadi Chipeta, Nancy Knight, Sergio Carmona, William MacLeod, Gayle Sherman, Yogan Pillay, Clement B Ndongmo, Bridget Mugisa, Annie Mwila, James McAuley, Peter J Chipimo, Wezi Kaonga, Dailess Nsofwa, Davy Nsama, Fales Zulu Mwamba, Crispin Moyo, Clement Phiri, Marie-Yolande Borget, Leonard Ya-Kouadio, Abo Kouame, Christiane A Adje-Toure, John Nkengasong
Pediatric human immunodeficiency virus (HIV) infection remains an important public health issue in resource-limited settings. In 2015, 1.4 million children aged <15 years were estimated to be living with HIV (including 170,000 infants born in 2015), with the vast majority living in sub-Saharan Africa (1). In 2014, 150,000 children died from HIV-related causes worldwide (2). Access to timely HIV diagnosis and treatment for HIV-infected infants reduces HIV-associated mortality, which is approximately 50% by age 2 years without treatment (3)...
November 25, 2016: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/27879149/increasing-amount-of-amyloid-are-associated-with-the-severity-of-clinical-features-in-hereditary-gelsolin-agel-amyloidosis
#18
Tiia Pihlamaa, Sinikka Suominen, Sari Kiuru-Enari, Maarit Tanskanen
BACKGROUND: Patients with hereditary gelsolin (AGel) amyloidosis (HGA) present with hanging skin (cutis laxa) and bilateral cranial neuropathy, and require symptomatic plastic surgery. Our clinical observation of tissue fragility prompted us to design a prospective study. METHODS: Twenty-nine patients with HGA undergoing surgery were interviewed and clinically examined. The height and thickness of skin folds in standard anatomical localizations were measured. The presence and distribution of amyloid in skin samples were analyzed using Congo red staining and immunohistochemistry using antibodies against gelsolin amyloid (AGel) subunit...
December 2016: Amyloid: the International Journal of Experimental and Clinical Investigation
https://www.readbyqxmd.com/read/27879120/network-based-analysis-of-a-small-ebola-outbreak
#19
Mark G Burch, Karly A Jacobsen, Joseph H Tien, Grzegorz A Rempala
We present a method for estimating epidemic parameters in network-based stochastic epidemic models when the total number of infections is assumed to be small. We illustrate the method by reanalyzing the data from the 2014 Democratic Republic of the Congo (DRC) Ebola outbreak described in Maganga et al. (2014).
February 1, 2017: Mathematical Biosciences and Engineering: MBE
https://www.readbyqxmd.com/read/27878461/crimean-congo-hemorrhagic-fever-among-children-in-iran
#20
Dalileh Aslani, Mostafa Salehi-Vaziri, Vahid Baniasadi, Tahmineh Jalali, Sanam Azad-Manjiri, Tahereh Mohammadi, Sahar Khakifirouz, Mehdi Fazlalipour
Crimean-Congo hemorrhagic fever (CCHF) is a viral zoonotic disease which is endemic in Iran. The etiological agent of CCHF is an RNA virus belonging to the genus Nairovirus of the family Bunyaviridae. CCHF virus (CCHFV) can be transmitted to humans through bites from infected ticks and direct contact with infected blood or tissues. Although the disease has been observed in different age groups, the rate of disease is lower in children and elderly. This study was designed to characterize CCHFV-infected children in Iran...
November 22, 2016: Archives of Virology
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