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https://www.readbyqxmd.com/read/28231617/characterization-of-staphylococcus-epidermidis-strains-isolated-from-industrial-cleanrooms-under-regular-routine-disinfection
#1
U Ribič, A Klančnik, B Jeršek
AIMS: The purpose of this study was the genotypic and phenotypic characterization of 57 strains of Staphylococcus epidermidis isolated from cleanroom environments, based on their biofilm formation and antimicrobial resistance profiles. METHODS AND RESULTS: Biofilm formation was investigated using real-time PCR (icaA, aap, bhp genes), the Congo red agar method, and the crystal violet assay. The majority of the strains (59.7%; 34/57) did not form biofilms according to the crystal violet assay, although the biofilm-associated genes were present in 94...
February 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28229332/the-role-of-riboflavin-in-decolourisation-of-congo-red-and-bioelectricity-production-using-shewanella-oneidensis-mr1-under-mfc-and-non-mfc-conditions
#2
Ola M Gomaa, Segun Fapetu, Godfrey Kyazze, Tajalli Keshavarz
Dissimilatory metal reducing bacteria can exchange electrons extracellularly and hold great promise for their use in simultaneous wastewater treatment and electricity production. This study investigated the role of riboflavin, an electron carrier, in the decolourisation of Congo red in microbial fuel cells (MFCs) using Shewanella oneidensis MR-1 as a model organism. The contribution of the membrane-bound protein MtrC to the decolourisation process was also investigated. Within the range of riboflavin concentrations tested, 20 µM was found to be the best with >95% of the dye (initial concentration 200 mg/L) decolourised in MFCs within 50 h compared to 90% in the case where no riboflavin was added...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28223751/secondary-cutaneous-amyloidosis-in-a-patient-with-mycosis-fungoides
#3
Chan Hee Nam, Min Kee Park, Mi Soo Choi, Seung Phil Hong, Byung Cheol Park, Myung Hwa Kim
Secondary cutaneous amyloidosis refers to clinically unapparent amyloid deposits within the skin in association with a pre-existing skin condition or skin tumors, such as basal cell carcinoma, porokeratosis, solar elastosis, Bowen's disease, and mycosis fungoides. A 70-year-old woman presented with a 6-month history of asymptomatic multiple yellowish plaques on both legs. She had been diagnosed with mycosis fungoides 7 years ago and was treated with psoralen and ultraviolet A radiation (PUVA) therapy, narrow-band ultraviolet B (UVB) therapy, and acitretin for 5 years...
February 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/28222303/organic-dye-removal-from-aqueous-solutions-by-hierarchical-calcined-ni-fe-layered-double-hydroxide-isotherm-kinetic-and-mechanism-studies
#4
Chunsheng Lei, Meng Pi, Panyong Kuang, Yingqing Guo, Fenge Zhang
Hierarchically porous nickel-iron-layered double hydroxide (NiFe-LDH) with a Ni(2+)/Fe(3+) molar ratio of 3 was successfully synthesised through a simple hydrothermal route. After calcination at 400°C, NiFe-LDH transformed into nickel-iron-layered double oxides (NiFe-LDO). The as-prepared samples were characterised through X-ray powder diffraction, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and nitrogen adsorption...
February 14, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28221129/new-mycobacterium-tuberculosis-complex-sublineage-brazzaville-congo
#5
Sven Malm, Laure S Ghoma Linguissi, Emmanuel M Tekwu, Jeannhey C Vouvoungui, Thomas A Kohl, Patrick Beckert, Anissa Sidibe, Sabine Rüsch-Gerdes, Igor K Madzou-Laboum, Sylvie Kwedi, Véronique Penlap Beng, Matthias Frank, Francine Ntoumi, Stefan Niemann
Tuberculosis is a leading cause of illness and death in Congo. No data are available about the population structure and transmission dynamics of the Mycobacterium tuberculosis complex strains prevalent in this central Africa country. On the basis of single-nucleotide polymorphisms detected by whole-genome sequencing, we phylogenetically characterized 74 MTBC isolates from Brazzaville, the capital of Congo. The diversity of the study population was high; most strains belonged to the Euro-American lineage, which split into Latin American Mediterranean, Uganda I, Uganda II, Haarlem, X type, and a new dominant sublineage named Congo type (n = 26)...
March 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28220714/is-maternal-education-a-social-vaccine-for-childhood-malaria-infection-a-cross-sectional-study-from-war-torn-democratic-republic-of-congo
#6
Cary Ma, Kasereka Masumbuko Claude, Zacharie Tsongo Kibendelwa, Hannah Brooks, Xiaonan Zheng, Michael Hawkes
In zones of violent conflict in the tropics, social disruption leads to elevated child mortality, of which malaria is the leading cause. Understanding the social determinants of malaria transmission may be helpful to optimize malaria control efforts. We conducted a cross-sectional study of healthy children aged 2 months to 5 years attending well-child and/or immunization visits in the Democratic Republic of Congo (DRC). Six hundred and forty-seven children were tested for malaria antigenemia by rapid diagnostic test and the accompanying parent or legal guardian simultaneously completed a survey questionnaire related to demographics, socioeconomic status, maternal education, as well as bednet use and recent febrile illness...
February 21, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28220300/application-of-confocal-laser-scanning-microscopy-for-the-diagnosis-of-amyloidosis
#7
Chiara Castellani, Marny Fedrigo, Anna Chiara Frigo, Mila Della Barbera, Gaetano Thiene, Marialuisa Valente, Fausto Adami, Annalisa Angelini
We analysed specificity and sensitivity of confocal laser microscopy (CLSM) on tissue sections for a diagnosis of amyloidosis, in an attempt to reduce technical errors and better standardise pathological diagnosis. We first set up a protocol for the use of CLSM on this type of specimen, using a group of 20 amyloid negative and 20 positive samples. Of all specimens, 2, 4 and 8-μm sections were cut. Sections were stained with Congo red (CR) and thioflavin-T (ThT) and observed by cross-polarised light microscopy (CR-PL), epifluorescence microscopy (CRF-epiFM and ThT-epiFM) and CLSM (CRF-CLSM and ThT-CLSM)...
February 20, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28219445/a-cross-sectional-study-of-the-income-sources-of-primary-care-health-workers-in-the-democratic-republic-of-congo
#8
Rishma Maini, David R Hotchkiss, Josephine Borghi
BACKGROUND: In the Democratic Republic of Congo (DRC), the state system to remunerate health workers is poorly functional, encouraging diversification of income sources and corruption. Given the central role that health workers play in health systems, policy-makers need to ensure health workers are remunerated in a way which best incentivises them to provide effective and good quality services. This study describes the different sources and quantities of income paid to primary care health workers in Equateur, Maniema, Kasai Occidental, Province Orientale and Kasai Oriental provinces...
February 20, 2017: Human Resources for Health
https://www.readbyqxmd.com/read/28218952/glomerular-hyperfiltration-is-strongly-correlated-with-age-in-congolese-children-with-sickle-cell-anaemia
#9
Michel Ntetani Aloni, René Makuala Ngiyulu, Pépé Mfutu Ekulu, Fiston IkwaNdol Mbutiwi, Jean Robert Makulo, Jean Lambert Gini-Ehungu, Nazaire Mangani Nseka, François Bompeka Lepira
AIM: Glomerular hyperfiltration is an early marker of sickle cell nephropathy and can lead to microalbuminuria and renal failure. Our aim was to identify the associated risk factors, as these could be of preventative importance. METHODS: We recruited 150 children with sickle cell anaemia (SCA), aged 2-18 years and living in Kinshasa, the Democratic Republic of Congo. Hyperfiltration and microalbuminuria were defined as an estimated glomerular filtration rate of less than 140 ml/min/1...
February 20, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28218698/genetic-assessment-of-african-swine-fever-isolates-involved-in-outbreaks-in-the-democratic-republic-of-congo-between-2005-and-2012-reveals-co-circulation-of-p72-genotypes-i-ix-and-xiv-including-19-variants
#10
Leopold K Mulumba-Mfumu, Jenna E Achenbach, Matthew R Mauldin, Linda K Dixon, Curé Georges Tshilenge, Etienne Thiry, Noelia Moreno, Esther Blanco, Claude Saegerman, Charles E Lamien, Adama Diallo
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated-except for Sardinia-followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein)...
February 18, 2017: Viruses
https://www.readbyqxmd.com/read/28215917/onchocerciasis-in-the-democratic-republic-of-congo-survey-of-knowledge-attitude-and-perception-in-bandundu-province
#11
Jean-Claude Makenga Bof, Dieudonne Mpunga, Emery N Soa, Fortunat Ntumba, Didier Bakajika, Michele E Murdoch, Yves Coppieters
A community-directed treatment with ivermectin (CDTI) for fighting onchocerciasis was started in 2003 in the hyperendemic province of Bandundu, Democratic Republic of Congo (DRC); such initiative was supported by the African Programme for Onchocerciasis Control (APOC). As the APOC stopped at the end of 2015, there was an urgent need to assess the sustainability of an ivermectin treatment. A cross-sectional survey of knowledge, attitude and perception was conducted to determine the awareness level of local population regarding the disease...
February 16, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28214190/biofilm-formation-by-staphylococcus-aureus-isolates-from-a-dental-clinic-in-konya-turkey
#12
Emrah Torlak, Emre Korkut, Ali T Uncu, Yağmur Şener
The ability of Staphylococcus aureus to form biofilm is considered to be a major virulence factor influencing its survival and persistence in both the environment and the host. Biofilm formation in S. aureus is most frequently associated with production of polysaccharide intercellular adhesion by ica operon-encoded enzymes. The present work aimed at evaluating the in vitro biofilm production and presence of the icaA and icaD genes in S. aureus isolates from a dental clinic in Konya, Turkey. The surfaces of inanimate objects were sampled over a period of six months...
February 14, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28214119/persistent-organic-pollutants-in-the-olifants-river-basin-south-africa-bioaccumulation-and-trophic-transfer-through-a-subtropical-aquatic-food-web
#13
Vera Verhaert, Nadine Newmark, Wendy D'Hollander, Adrian Covaci, Wynand Vlok, Victor Wepener, Abraham Addo-Bediako, Antoinette Jooste, Johannes Teuchies, Ronny Blust, Lieven Bervoets
This study investigates the trophic transfer of persistent organic pollutants (POPs: PCBs, PBDEs, OCPs and PFASs) in the subtropical aquatic ecosystem of the Olifants River Basin (South Africa) by means of trophic magnification factors (TMFs). Relative trophic levels were determined by stable isotope analysis. POP levels in surface water, sediment and biota were low. Only ∑DDTs levels in fish muscle (<LOQ-61ng/g ww) were comparable or higher than values from other temperate and tropical regions. Significant positive relationships between relative trophic level and PCB, DDT and HCH concentrations were observed so trophic levels play an important role in the movement of contaminants through the food web...
February 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28213810/how-to-tackle-natural-focal-infections-from-risk-assessment-to-vaccination-strategies
#14
Luca Busani, Alexander E Platonov, Onder Ergonul, Giovanni Rezza
Natural focal diseases are caused by biological agents associated with specific landscapes. The natural focus of such diseases is defined as any natural ecosystem containing the pathogen's population as an essential component. In such context, the agent circulates independently on human presence, and humans may become accidentally infected through contact with vectors or reservoirs. Some viruses (i.e., tick-borne encephalitis and Congo-Crimean hemorrhagic fever virus) are paradigmatic examples of natural focal diseases...
February 18, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28213150/preparation-of-the-go-pd-nanocomposite-and-its-application-for-the-degradation-of-organic-dyes-in-water
#15
Afshan Omidvar, Babak Jaleh, Mahmoud Nasrollahzadeh
A GO/Pd nanocomposite has been successfully synthesized applying a simple method via immobilizing Pd on the surface of the graphene oxide (GO). The GO/Pd nanocomposite was characterized by X-ray diffraction (XRD), Fourier transformed infrared (FT-IR) spectroscopy, Raman, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) techniques. The surface morphology of Pd NPs was viewed by TEM, the particles are spherical with an average particle size of 11nm...
February 9, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28210184/surgical-treatment-of-a-rare-case-of-bilateral-ptosis-due-to-localized-ocular-amyloidosis
#16
Gianluca Scuderi, Solmaz Abdolrahimzadeh, Antonietta Troccola, Marco Mazzocchi, Santi Maria Recupero
We describe a rare case of a 31-year old woman with bilateral ptosis due to localized amyloidosis. She referred a nine-year history of ptosis and surgical treatment with frontalis suspension three years previously. Following complete ophthalmological examination and evaluation of the ptosis we carried out tarsal and fornix biopsy, which revealed accumulation of a weakly eosinophilic amyloid positive substance. We performed surgical correction using the levator aponeurosis-Müller's muscle complex re-adaptation technique and amyloid substance debulking in all the palpebral layers in the left eye...
July 2016: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
https://www.readbyqxmd.com/read/28202798/high-prevalence-and-diversity-of-hepatitis-viruses-in-suspected-cases-of-yellow-fever-in-the-democratic-republic-of-congo
#17
Sheila Makiala-Mandanda, Frédéric Le Gal, Nadine Ngwaka-Matsung, Steve Ahuka-Mundeke, Richard Onanga, Berthold Bivigou-Mboumba, Elisabeth Pukuta-Simbu, Athenaïs Gerber, Jessica L Abbate, Dieudonné Mwamba, Nicolas Berthet, Eric Maurice Leroy, Jean-Jacques Muyembe-Tamfum, Pierre Becquart
The majority of patients with acute febrile jaundice (>95%) identified through a yellow fever surveillance program in the Democratic Republic of Congo (DRC) test negative for antibodies against yellow fever virus. However, no etiological investigation has ever been carried out on these patients. Here, we tested for hepatitis A (HAV), B (HBV), C (HCV), D (HDV) and E (HEV) viruses, all of which can cause acute febrile jaundice, in patients included in the yellow fever surveillance program in the DRC. On a total of 498 serum samples collected from suspected cases of yellow fever from January 2003 to January 2012, ELISA techniques were used to screen for antibodies against HAV (IgM) and HEV (IgM) and for antigens and antibodies against HBV (HBsAg and anti-HBc IgM), HCV and HDV...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28201963/inhibition-of-amyloid-like-fibril-formation-of-trypsin-by-red-wines
#18
Márta Kotormán, Phanindra Babu Kasi, László Halász, Attila Borics
The aim of the present study was to examine the potential role and applicability of dietary supplements in reducing the risk of development of amyloid diseases associated with the gastrointestinal tract, such as type II diabetes. Trypsin, a well-known serine protease was used as a model protein in our experiments. The effect of various red wines on the formation of amyloid-like fibrils of trypsin was studied in vitro, in aqueous ethanol, at pH 7.0. Turbidity measurements, aggregation kinetics experiments, Congo red binding assays and electronic circular dichroism spectroscopic measurements were used to follow the aggregation process in the presence or absence of various red wines...
February 14, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28195198/the-gadolinium-gd-3-and-tin-sn-4-co-doped-bifeo3-nanoparticles-as-new-solar-light-active-photocatalyst
#19
Syed Irfan, Syed Rizwan, Yang Shen, Liangliang Li, Asfandiyar, Sajid Butt, Ce-Wen Nan
The process of photocatalysis is appealing to huge interest motivated by the great promise of addressing current energy and environmental issues through converting solar light directly into chemical energy. However, an efficient solar energy harvesting for photocatalysis remains a critical challenge. Here, we reported a new full solar spectrum driven photocatalyst by co-doping of Gd(3+) and Sn(4+) into A and B-sites of BiFeO3 simultaneously. The co-doping of Gd(3+) and Sn(4+) played a key role in hampering the recombination of electron-hole pairs and shifted the band-gap of BiFeO3 from 2...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28192518/infant-feeding-practices-and-diarrhoea-in-sub-saharan-african-countries-with-high-diarrhoea-mortality
#20
Felix A Ogbo, Kingsley Agho, Pascal Ogeleka, Sue Woolfenden, Andrew Page, John Eastwood
BACKGROUND: The impacts of optimal infant feeding practices on diarrhoea have been documented in some developing countries, but not in countries with high diarrhoea mortality as reported by the World Health Organisation/United Nations Children's Fund. We aimed to investigate the association between infant feeding practices and diarrhoea in sub-Saharan African countries with high diarrhoea mortality. METHOD: The study used the most recent Demographic and Health Survey datasets collected in nine sub-Saharan African countries with high diarrhoea mortality, namely: Burkina Faso (2010, N = 9,733); Demographic Republic of Congo (2013; N = 10,458); Ethiopia (2013, N = 7,251); Kenya (2014, N = 14,034); Mali (2013, N = 6,365); Niger (2013, N = 7,235); Nigeria (2013, N = 18,539); Tanzania (2010, N = 5,013); and Uganda (2010, N = 4,472)...
2017: PloS One
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