keyword
MENU ▼
Read by QxMD icon Read
search

Erf1

keyword
https://www.readbyqxmd.com/read/28626940/overexpression-of-hevea-brasiliensis-ethylene-response-factor-hberf-ixc5-enhances-growth-tolerance-to-abiotic-stress-and-affects-laticifer-differentiation
#1
Retno Lestari, Maryannick Rio, Florence Martin, Julie Leclercq, Natthakorn Woraathasin, Sandrine Roques, Florence Dessailly, Anne Clément-Vidal, Christine Sanier, Denis Fabre, Sémi Melliti, Sony Suharsono, Pascal Montoro
Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting stress for better latex production. This paper set out to establish and characterize rubber transgenic lines overexpressing HbERF-IXc5. Overexpression of HbERF-IXc5 dramatically enhanced plant growth and enabled plants to maintain some ecophysiological parameters in response to abiotic stress such as water deficit, cold and salt treatments...
June 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28620159/main-and-epistatic-loci-studies-in-soybean-for-sclerotinia-sclerotiorum-resistance-reveal-multiple-modes-of-resistance-in-multi-environments
#2
Tara C Moellers, Arti Singh, Jiaoping Zhang, Jae Brungardt, Mehdi Kabbage, Daren S Mueller, Craig R Grau, Ashish Ranjan, Damon L Smith, R V Chowda-Reddy, Asheesh K Singh
Genome-wide association (GWAS) and epistatic (GWES) studies along with expression studies in soybean [Glycine max (L.) Merr.] were leveraged to dissect the genetics of Sclerotinia stem rot (SSR) [caused by Sclerotinia sclerotiorum (Lib.) de Bary], a significant fungal disease causing yield and quality losses. A large association panel of 466 diverse plant introduction accessions were phenotyped in multiple field and controlled environments to: (1) discover sources of resistance, (2) identify SNPs associated with resistance, and (3) determine putative candidate genes to elucidate the mode of resistance...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28576486/construction-of-an-inducible-stable-cell-line-for-efficient-incorporation-of-unnatural-amino-acids-in-mammalian-cells
#3
Ziwei Zhang, Huan Xu, Longlong Si, Yi Chen, Bo Zhang, Yan Wang, Yiming Wu, Xueying Zhou, Lihe Zhang, Demin Zhou
The genetic incorporation of unnatural amino acids (Uaas) with defined properties into proteins at designated sites represents an extremely powerful tool for protein engineering. However, the efficient incorporation of Uaas in response to the amber stop codon in mammalian cells remains a substantial challenge due to the competition from release factor 1(RF1). Addressing this challenge will greatly broaden the power and scope of this technology. Here, we chose the eRF1 mutant, which can selectively enhance Uaa incorporation in response to the amber codon without increasing the readthrough of the opal and ochre codons...
May 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28567122/a-functional-role-for-the-monomethylated-gln-51-and-lys-53-residues-of-the-49ggqtk53-motif-of-el42-from-human-80s-ribosomes
#4
Stéphanie Eustache, Jean-Bernard Créchet, Tahar Bouceba, Jun-Ichi Nakayama, Mayo Tanaka, Mieko Suzuki, Anne Woisard, Pierre Tuffery, Soria Baouz, Codjo Hountondji
BACKGROUND: We have previously demonstrated that the eukaryote-specific ribosomal protein eL42 of the human 80S ribosome contains seven monomethylated residues, among which are the Gln-51 and Lys-53 residues contained in the 47GFGGQTK53 sequence conserved in all eukaryotic 80S ribosomes. This sequence contains the methylated and universally conserved GGQ motif common for all class-1 translation termination factors responsible for stop codon recognition and for triggering the hydrolysis of the P site-bound peptidyl-tRNA...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28552050/readthrough-of-scn5a-nonsense-mutations-r1623x-and-s1812x-question-gene-therapy-in-brugada-syndrome
#5
Siyong Teng, Jian Huang, Zhan Gao, Jie Hao, Yuejin Yang, Shu Zhang, Jielin Pu, Rutai Hui, Yongjian Wu, Zheng Fan
PURPOSE: Readthrough of nonsense mutation are used as gene-specific treatment in some genetic disease. Response to readthrough treatment is determined by readthrough efficiency of various nonsense mutations. In this manuscript, we aimed to explore the harmful effect of nonsense mutation suppressions. METHODS: HEK293 cells were transfected with two SCN5A (encode cardiac Na+ channel) nonsense mutations, p.R1623X and p.S1812X. We applied two readthrough-enhancing methods (either aminoglycosides or by a siRNA targeting eukaryotic release factor eRF3a (a GTPase that binds eRF1)) to suppress these SCN5A nonsense mutations...
May 28, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28542545/a-first-insight-into-the-involvement-of-phytohormones-pathways-in-coffee-resistance-and-susceptibility-to-colletotrichum-kahawae
#6
Inês Diniz, Andreia Figueiredo, Andreia Loureiro, Dora Batista, Helena Azinheira, Vítor Várzea, Ana Paula Pereira, Elijah Gichuru, Pilar Moncada, Leonor Guerra-Guimarães, Helena Oliveira, Maria do Céu Silva
Understanding the molecular mechanisms underlying coffee-pathogen interactions are of key importance to aid disease resistance breeding efforts. In this work the expression of genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) pathways were studied in hypocotyls of two coffee varieties challenged with the hemibiotrophic fungus Colletotrichum kahawae, the causal agent of Coffee Berry Disease. Based on a cytological analysis, key time-points of the infection process were selected and qPCR was used to evaluate the expression of phytohormones biosynthesis, reception and responsive-related genes...
2017: PloS One
https://www.readbyqxmd.com/read/28467675/ribosome-bound-pub1-modulates-stop-codon-decoding-during-translation-termination-in-yeast
#7
Valery N Urakov, Olga V Mitkevich, Irina V Safenkova, Michael D Ter-Avanesyan
In eukaryotes, termination of translation is controlled by polypeptide chain release factors eRF1 and eRF3, of which the former recognizes nonsense codons, while the latter interacts with eRF1 and stimulates polypeptide release from the ribosome in a GTP- dependent manner, and ABCE1, which facilitates ribosome recycling. In this work, we demonstrate that Pub1, a yeast protein known to be involved in stress granule formation, regulation of gene expression, and organization of the tubulin cytoskeleton, also plays a role in translation termination...
May 3, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28457996/exploring-contacts-of-erf1-with-the-3-terminus-of-the-p-site-trna-and-mrna-stop-signal-in-the-human-ribosome-at-various-translation-termination-steps
#8
Konstantin N Bulygin, Dmitri M Graifer, Codjo Hountondji, Ludmila Yu Frolova, Galina G Karpova
Here we employed site-directed cross-linking with the application of tRNA and mRNA analogues bearing an oxidized ribose at the 3'-terminus to investigate mutual arrangement of the main components of translation termination complexes formed on the human 80S ribosome bound with P site deacylated tRNA using eRF1•eRF3•GTP or eRF1 alone. In addition, we applied a model complex obtained in the same way with eRF1•eRF3•GMPPNP. We found that eRF3 content in the complexes with GTP and GMPPNP is similar, proving that eRF3 does not leave the ribosome after GTP hydrolysis...
April 27, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28174592/integration-of-ethylene-and-light-signaling-affects-hypocotyl-growth-in-arabidopsis
#9
REVIEW
Yanwen Yu, Rongfeng Huang
As an ideal model for studying ethylene effects on cell elongation, Arabidopsis hypocotyl growth is widely used due to the unique characteristic that ethylene stimulates hypocotyl elongation in the light but inhibits it in the dark. Although the contrasting effect of ethylene on hypocotyl growth has long been known, the molecular basis of this effect has only gradually been identified in recent years. In the light, ethylene promotes the expression of PHYTOCHROME INTERACTING FACTOR 3 (PIF3) and the degradation of ELONGATED HYPOCOTYL 5 (HY5) protein, thus stimulating hypocotyl growth...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28134793/trm112-a-protein-activator-of-methyltransferases-modifying-actors-of-the-eukaryotic-translational-apparatus
#10
REVIEW
Gabrielle Bourgeois, Juliette Létoquart, Nhan van Tran, Marc Graille
Post-transcriptional and post-translational modifications are very important for the control and optimal efficiency of messenger RNA (mRNA) translation. Among these, methylation is the most widespread modification, as it is found in all domains of life. These methyl groups can be grafted either on nucleic acids (transfer RNA (tRNA), ribosomal RNA (rRNA), mRNA, etc.) or on protein translation factors. This review focuses on Trm112, a small protein interacting with and activating at least four different eukaryotic methyltransferase (MTase) enzymes modifying factors involved in translation...
January 27, 2017: Biomolecules
https://www.readbyqxmd.com/read/28118091/paraburkholderia-phytofirmans-psjn-protects-arabidopsis-thaliana-against-a-virulent-strain-of-pseudomonas-syringae-through-the-activation-of-induced-resistance
#11
Tania Timmermann, Grace Armijo, Raúl Donoso, Aldo Seguel, Loreto Holuigue, Bernardo González
Paraburkholderia phytofirmans PsJN is a plant growth-promoting rhizobacterium (PGPR) that stimulates plant growth and improves tolerance to abiotic stresses. This study analyzed whether strain PsJN can reduce plant disease severity and proliferation of the virulent strain Pseudomonas syringae pv. tomato DC3000, in Arabidopsis plants, through the activation of induced resistance. Arabidopsis plants previously exposed to strain PsJN showed a reduction in disease severity and pathogen proliferation in leaves compared with noninoculated, infected plants...
March 16, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28062855/expression-of-the-erf1-translation-termination-factor-is-controlled-by-an-autoregulatory-circuit-involving-readthrough-and-nonsense-mediated-decay-in-plants
#12
Tünde Nyikó, Andor Auber, Levente Szabadkai, Anna Benkovics, Mariann Auth, Zsuzsanna Mérai, Zoltán Kerényi, Andrea Dinnyés, Ferenc Nagy, Dániel Silhavy
When a ribosome reaches a stop codon, the eukaryotic Release Factor 1 (eRF1) binds to the A site of the ribosome and terminates translation. In yeasts and plants, both over- and underexpression of eRF1 lead to altered phenotype indicating that eRF1 expression should be strictly controlled. However, regulation of eRF1 level is still poorly understood. Here we show that expression of plant eRF1 is controlled by a complex negative autoregulatory circuit, which is based on the unique features of the 3΄untranslated region (3΄UTR) of the eRF1-1 transcript...
April 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27986851/activation-mode-of-the-eukaryotic-m2g10-trna-methyltransferase-trm11-by-its-partner-protein-trm112
#13
Gabrielle Bourgeois, Julien Marcoux, Jean-Michel Saliou, Sarah Cianférani, Marc Graille
Post-transcriptional and post-translational modifications of factors involved in translation are very important for the control and accuracy of protein biosynthesis. Among these factors, tRNAs harbor the largest variety of grafted chemical structures, which participate in tRNA stability or mRNA decoding. Here, we focused on Trm112 protein, which associates with four different eukaryotic methyltransferases modifying tRNAs (Trm9 and Trm11) but also 18S-rRNA (Bud23) and translation termination factor eRF1 (Mtq2)...
February 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27940554/rna-helicase-ddx19-stabilizes-ribosomal-elongation-and-termination-complexes
#14
Tatiana Mikhailova, Ekaterina Shuvalova, Alexander Ivanov, Denis Susorov, Alexey Shuvalov, Peter M Kolosov, Elena Alkalaeva
The human DEAD-box RNA-helicase DDX19 functions in mRNA export through the nuclear pore complex. The yeast homolog of this protein, Dbp5, has been reported to participate in translation termination. Using a reconstituted mammalian in vitro translation system, we show that the human protein DDX19 is also important for translation termination. It is associated with the fraction of translating ribosomes. We show that DDX19 interacts with pre-termination complexes (preTCs) in a nucleotide-dependent manner. Furthermore, DDX19 increases the efficiency of termination complex (TC) formation and the peptide release in the presence of eukaryotic release factors...
December 9, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27933082/rna-seq-transcriptome-response-of-flax-linum-usitatissimum-l-to-the-pathogenic-fungus-fusarium-oxysporum-f-sp-lini
#15
Leonardo Galindo-González, Michael K Deyholos
Fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. Along with rust, fusarium wilt has become an important factor in flax production worldwide. Resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. This interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform breeding programs on potential candidate genes to evaluate or improve resistance across cultivars...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27863242/decoding-mammalian-ribosome-mrna-states-by-translational-gtpase-complexes
#16
Sichen Shao, Jason Murray, Alan Brown, Jack Taunton, V Ramakrishnan, Ramanujan S Hegde
In eukaryotes, accurate protein synthesis relies on a family of translational GTPases that pair with specific decoding factors to decipher the mRNA code on ribosomes. We present structures of the mammalian ribosome engaged with decoding factor⋅GTPase complexes representing intermediates of translation elongation (aminoacyl-tRNA⋅eEF1A), termination (eRF1⋅eRF3), and ribosome rescue (Pelota⋅Hbs1l). Comparative analyses reveal that each decoding factor exploits the plasticity of the ribosomal decoding center to differentially remodel ribosomal proteins and rRNA...
November 17, 2016: Cell
https://www.readbyqxmd.com/read/27830682/-identification-of-new-genes-that-affect-psi-prion-toxicity-in-saccharomyces-cerevisiae-yeast
#17
A G Matveenko, M V Belousov, S A Bondarev, S E Moskalenko, G A Zhouravleva
Translation termination is an important step in gene expression. Its correct processing is governed by eRF1 (Sup45) and eRF3 (Sup35) proteins. In Saccharomyces cerevisiae, mutations in the corresponding genes, as well as Sup35 aggregation in [PSI^(+)] cells that propagate the prion form of Sup35 lead to inaccurate stop codon recognition and, consequently, nonsense suppression. The presence of stronger prion variants results in the more efficient suppression of nonsense mutations. Previously, we proposed a synthetic lethality test that enables the identification of genes that may influence either translation termination factors or [PSI^(+)] manifestation...
September 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/27787902/ubc18-mediates-erf1-degradation-under-light-dark-cycles
#18
Mei-Chun Cheng, Wen-Chieh Kuo, Yi-Ming Wang, Hsing-Yu Chen, Tsan-Piao Lin
Ethylene Response Factor 1 (ERF1) plays a crucial role in biotic and abiotic stress responses. Previous studies have shown that ERF1 regulates stress-responsive gene expression by binding to different cis-acting elements in response to various stress signals. ERF1 was also reported to be unstable in the dark, and it regulates hypocotyl elongation. Here, we elucidated the mechanism underlying degradation of ERF1. Yeast two-hybrid screening showed that UBIQUITIN-CONJUGATING ENZYME 18 (UBC18) interacted with ERF1...
October 27, 2016: New Phytologist
https://www.readbyqxmd.com/read/27626604/evaluation-of-different-oral-formulations-of-clindamycin-extended-release-in-dogs
#19
RANDOMIZED CONTROLLED TRIAL
E Ortega, L Gutiérrez, M J Bernad, F Salmerón, I Juárez, D Vargas
The purpose of this study was to evaluate the pharmacokinetics of extended-release formulations (ERFs) of clindamycin with polymeric-based matrices. In a crossover study, 21 healthy adult dogs were randomly assigned (in groups of 7) to receive a single oral dose (20 mg/kg) of clindamycin without excipients (control) or an extended-release formulation containing clindamycin+Hydroxypropyl methylcellulose (HPMC)+poloxamer at a ratio of 1 : 0.04 : 0.5 (ERF1) or containing clindamycin+HPMC+acrylic acid polymer (AAP) at the same proportions (ERF2)...
January 2017: Drug Research
https://www.readbyqxmd.com/read/27593378/an-unprecedented-non-canonical-nuclear-genetic-code-with-all-three-termination-codons-reassigned-as-sense-codons
#20
Kristína Záhonová, Alexei Y Kostygov, Tereza Ševčíková, Vyacheslav Yurchenko, Marek Eliáš
A limited number of non-canonical genetic codes have been described in eukaryotic nuclear genomes. Most involve reassignment of one or two termination codons as sense ones [1-4], but no code variant is known that would have reassigned all three termination codons. Here, we describe such a variant that we discovered in a clade of trypanosomatids comprising nominal Blastocrithidia species. In these protists, UGA has been reassigned to encode tryptophan, while UAG and UAA (UAR) have become glutamate encoding. Strikingly, UAA and, less frequently, UAG also serve as bona fide termination codons...
September 12, 2016: Current Biology: CB
keyword
keyword
8036
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"