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https://www.readbyqxmd.com/read/29596001/identification-of-candidate-genes-involved-in-the-salt-tolerance-of-date-palm-phoenix-dactylifera-l-based-on-a-yeast-functional-bioassay
#1
Himanshu V Patankar, Ibtisam Al-Harrasi, Rashid Al-Yahyai, Mahmoud W Yaish
Although date palm is a relatively salt-tolerant plant, the molecular basis of this tolerance is complex and poorly understood. Therefore, this study aimed to identify the genes involved in salinity tolerance using a basic yeast functional bioassay. To achieve this, a date palm cDNA library was overexpressed in Saccharomyces cerevisiae cells. The expression levels of selected genes that make yeast cells tolerant to salt were subsequently validated in the leaf and root tissues of date palm seedlings using a quantitative PCR method...
March 29, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29566164/lml1-encoding-a-conserved-eukaryotic-release-factor-1-protein-regulates-cell-death-and-pathogen-resistance-by-forming-a-conserved-complex-with-spl33-in-rice
#2
Peng Qin, Shijun Fan, Luchang Deng, Guangrong Zhong, Siwei Zhang, Meng Li, Weilan Chen, Geling Wang, Bin Tu, Yuping Wang, Xuewei Chen, Bingtian Ma, Shigui Li
Lesion mimic mutants are powerful tools for unveiling the molecular connections between cell death and pathogen resistance. Various proteins responsible for lesion mimics have been identified, however, the mechanism underlying lesion formation and pathogen resistance are still unknown. Here, we identify a lesion mimic mutant in rice, lesion mimic leaf 1 (lml1). The lml1 mutant exhibited abnormal cell death and resistance to both bacterial blight and rice blast. LML1 is expressed in all types of leaf cells, and encodes a novel eukaryotic release factor 1 (eRF1) protein located in the endoplasmic reticulum (ER)...
March 15, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29552555/stress-inducible-overexpression-of-athdg11-leads-to-improved-drought-and-salt-stress-tolerance-in-peanut-arachis-hypogaea-l
#3
Jayanna N Banavath, Thammineni Chakradhar, Varakumar Pandit, Sravani Konduru, Krishna K Guduru, Chandra S Akila, Sudhakar Podha, Chandra O R Puli
Peanut is an important oilseed and food legume cultivated as a rain-fed crop in semi-arid tropics. Drought and high salinity are the major abiotic stresses limiting the peanut productivity in this region. Development of drought and salt tolerant peanut varieties with improved yield potential using biotechnological approach is highly desirable to improve the peanut productivity in marginal geographies. As abiotic stress tolerance and yield represent complex traits, engineering of regulatory genes to produce abiotic stress-resilient transgenic crops appears to be a viable approach...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29515596/genomic-sequencing-of-japanese-plum-prunus-salicina-lindl-mutants-provides-a-new-model-for-rosaceae-fruit-ripening-studies
#4
Angel Fernandez I Marti, Christopher A Saski, George A Manganaris, Ksenija Gasic, Carlos H Crisosto
It has recently been described that the Japanese plum "Santa Rosa" bud sport series contains variations in ripening pattern: climacteric, suppressed-climacteric and non-climacteric types. This provides an interesting model to study the role of ethylene and other key mechanisms governing fruit ripening, softening and senescence. The aim of the current study was to investigate such differences at the genomic level, using this series of plum bud sports, with special reference to genes involved in ethylene biosynthesis, signal transduction, and sugar metabolism...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29467500/a-tomato-mads-box-protein-slcmb1-regulates-ethylene-biosynthesis-and-carotenoid-accumulation-during-fruit-ripening
#5
Jianling Zhang, Zongli Hu, Qiyuan Yao, Xuhu Guo, Vanluc Nguyen, Fenfen Li, Guoping Chen
The MADS-box transcription factors play essential roles in many physiological and biochemical processes of plants, especially in fruit ripening. Here, a tomato MADS-box gene, SlCMB1, was isolated. SlCMB1 expression declined with the fruit ripening from immature green to B + 7 (7 days after Breaker) fruits in the wild type (WT) and was lower in Nr and rin mutants fruits. Tomato plants with reduced SlCMB1 mRNA displayed delayed fruit ripening, reduced ethylene production and carotenoid accumulation. The ethylene production in SlCMB1-RNAi fruits decreased by approximately 50% as compared to WT...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29325104/eukaryotic-translational-termination-efficiency-is-influenced-by-the-3-nucleotides-within-the-ribosomal-mrna-channel
#6
Andrew G Cridge, Caillan Crowe-McAuliffe, Suneeth F Mathew, Warren P Tate
When a stop codon is at the 80S ribosomal A site, there are six nucleotides (+4 to +9) downstream that are inferred to be occupying the mRNA channel. We examined the influence of these downstream nucleotides on translation termination success or failure in mammalian cells at the three stop codons. The expected hierarchy in the intrinsic fidelity of the stop codons (UAA>UAG>UGA) was observed, with highly influential effects on termination readthrough mediated by nucleotides at position +4 and position +8...
February 28, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29298914/atomic-mutagenesis-of-stop-codon-nucleotides-reveals-the-chemical-prerequisites-for-release-factor-mediated-peptide-release
#7
Thomas Philipp Hoernes, Nina Clementi, Michael Andreas Juen, Xinying Shi, Klaus Faserl, Jessica Willi, Catherina Gasser, Christoph Kreutz, Simpson Joseph, Herbert Lindner, Alexander Hüttenhofer, Matthias David Erlacher
Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs). Whereas in bacteria, RF1 and RF2 promote termination at UAA/UAG and UAA/UGA stop codons, respectively, eukaryotes only depend on one RF (eRF1) to initiate peptide release at all three stop codons. Based on several structural as well as biochemical studies, interactions between mRNA, tRNA, and rRNA have been proposed to be required for stop codon recognition...
January 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29238352/overexpression-of-erf1-v-from-haynaldia-villosa-can-enhance-the-resistance-of-wheat-to-powdery-mildew-and-increase-the-tolerance-to-salt-and-drought-stresses
#8
Liping Xing, Zhaocan Di, Wenwu Yang, Jiaqian Liu, Meina Li, Xiaojuan Wang, Chaofan Cui, Xiaoyun Wang, Xiue Wang, Ruiqi Zhang, Jin Xiao, Aizhong Cao
The APETALA 2/Ethylene-responsive element binding factor (AP2/ERF) transcription factor gene family is widely involved in the biotic and abiotic stress regulation. Haynaldia villosa (VV, 2 n = 14), a wild species of wheat, is a potential gene pool for wheat improvement. H. villosa confers high resistance to several wheat diseases and high tolerance to some abiotic stress. In this study, ERF1-V , an ethylene-responsive element-binding factor gene of the AP2/ERF transcription factor gene family from wild H. villosa , was cloned and characterized...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29193778/a-substitution-mutation-in-ospelota-confers-bacterial-blight-resistance-by-activating-the-salicylic-acid-pathway
#9
Xiao-Bo Zhang, Bao-Hua Feng, Hui-Mei Wang, Xia Xu, Yong-Feng Shi, Yan He, Zheng Chen, Atul Prakash Sathe, Lei Shi, Jian-Li Wu
We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed splHM47 ). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype...
February 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29139201/defining-the-protein-complexome-of-translation-termination-factor-erf1-identification-of-four-novel-erf1-containing-complexes-that-range-from-20s-to-57s-in-size
#10
Clyde L Denis, Roy Richardson, Shiwha Park, Chongxu Zhang, Wen Xi, Thomas M Laue, Xin Wang
The eukaryotic eRF1 translation termination factor plays an important role in recognizing stop codons and initiating the end to translation. However, which exact complexes contain eRF1 and at what abundance is not clear. We have used analytical ultracentrifugation with fluorescent detection system to identify the protein complexome of eRF1 in the yeast Saccharomyces cerevisiae. In addition to eRF1 presence in translating polysomes, we found that eRF1 associated with five other macromolecular complexes: 77S, 57S, 39S, 28S, and 20S in size...
November 15, 2017: Proteins
https://www.readbyqxmd.com/read/29127299/origin-of-the-omnipotence-of-eukaryotic-release-factor-1
#11
Christoffer Lind, Ana Oliveira, Johan Åqvist
Termination of protein synthesis on the ribosome requires that mRNA stop codons are recognized with high fidelity. This is achieved by specific release factor proteins that are very different in bacteria and eukaryotes. Hence, while there are two release factors with overlapping specificity in bacteria, the single omnipotent eRF1 release factor in eukaryotes is able to read all three stop codons. This is particularly remarkable as it is able to select three out of four combinations of purine bases in the last two codon positions...
November 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/29096083/gene-network-underlying-the-response-of-harvested-pepper-to-chilling-stress
#12
Sun-Young Shin, Me-Hea Park, Ji-Weon Choi, Ji-Gang Kim
Cold storage is an effective postharvest control strategy to maintain the freshness of vegetables by suppressing respiration. However, subtropical plants including pepper (Capsicum annuum L.) undergo chilling injury. To better understand the molecular mechanisms involved in preventing chilling injury, transcriptome profiling analysis of peppers stored in a cold chamber and treated with 50μM methyl jasmonate (MeJA) and 1μLL(-1) 1-methylcyclopropene as an ethylene reaction inhibitor was performed. A total of 240, 470, and 290 genes were upregulated and 184, 291, and 219 genes down-regulated in cold-, MeJA- and 1-methylcyclopropene-treated peppers, respectively...
October 28, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28954403/textile-hemp-vs-salinity-insights-from-a-targeted-gene-expression-analysis
#13
Gea Guerriero, Marc Behr, Jean-Francois Hausman, Sylvain Legay
Soil salinity is a serious threat to agriculture, because it compromises biomass production and plant productivity, by negatively affecting the vegetative growth and development of plants. Fiber crops like textile hemp (Cannabis sativa L.) are important natural resources that provide, sustainably, both cellulosic and woody fibers for industry. In this work, the response to salinity (200 mM NaCl) of a fiber variety of hemp (Santhica 27) was studied using quantitative real-time PCR. The responses of plantlets aged 15 days were analyzed by microscopy and by measuring the changes in expression of cell wall-related genes, as well as in the general response to exogenous constraints...
September 26, 2017: Genes
https://www.readbyqxmd.com/read/28934298/transcriptome-analysis-of-callery-pear-pyrus-calleryana-reveals-a-comprehensive-signalling-network-in-response-to-alternaria-alternata
#14
Jialiang Kan, Tingli Liu, Na Ma, Hui Li, Xiaogang Li, Jinyan Wang, Baolong Zhang, Youhong Chang, Jing Lin
The pear is an important temperate fruit worldwide that is produced by a group of species in the genus Pyrus. Callery pear (Pyrus calleryana Decne) is characterized by high resistance to multiple diseases, good adaptability, and high ornamental value, and is therefore widely planted in pear orchards for edible fruit production or as stock. Plant pathogens are a major threat to pear yield. Black spot disease, caused by the filamentous fungus Alternaria alternata, is one of the most serious diseases in pear. Elucidation of resistant genes to black spot disease is extremely important for understanding the underlying mechanisms as well as for the development of resistant cultivars...
2017: PloS One
https://www.readbyqxmd.com/read/28889908/resistance-of-fusarium-poae-in-arabidopsis-leaves-requires-mainly-functional-ja-and-et-signaling-pathways
#15
María Inés Dinolfo, Eliana Castañares, Sebastián A Stenglein
Fusarium poae has been considered as a minor species among those that cause the FHB disease but in recent years several researchers have documented a high frequency of occurrence in several crops. We evaluated the ability of F. poae to produce symptoms in A. thaliana leaves. Moreover, we analyzed the defense of A. thaliana against F. poae using SA, JA, and ET mutants and we monitored the expression level of genes involved in the main signaling pathways related to plant defense. Symptoms were observed in the inoculated leaves demonstrating the ability of F...
October 2017: Fungal Biology
https://www.readbyqxmd.com/read/28878788/transcriptomic-analysis-reveals-candidate-genes-for-female-sterility-in-pomegranate-flowers
#16
Lina Chen, Jie Zhang, Haoxian Li, Juan Niu, Hui Xue, Beibei Liu, Qi Wang, Xiang Luo, Fuhong Zhang, Diguang Zhao, Shangyin Cao
Pomegranate has two types of flowers on the same plant: functional male flowers (FMF) and bisexual flowers (BF). BF are female-fertile flowers that can set fruits. FMF are female-sterile flowers that fail to set fruit and that eventually drop. The putative cause of pomegranate FMF female sterility is abnormal ovule development. However, the key stage at which the FMF pomegranate ovules become abnormal and the mechanism of regulation of pomegranate female sterility remain unknown. Here, we studied ovule development in FMF and BF, using scanning electron microscopy to explore the key stage at which ovule development was terminated and then analyzed genes differentially expressed (differentially expressed genes - DEGs) between FMF and BF to investigate the mechanism responsible for pomegranate female sterility...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28649000/suppression-of-the-mads-box-gene-slmbp8-accelerates-fruit-ripening-of-tomato-solanum-lycopersicum
#17
Wencheng Yin, Zongli Hu, Baolu Cui, Xuhu Guo, Jingtao Hu, Zhiguo Zhu, Guoping Chen
MADS-box genes encode important transcription factors that are involved in many biological processes of plants, including fruit ripening. In our research, a MADS-box gene, SlMBP8, was identified, and its tissue-specific expression profiles were analysed. SlMBP8 was highly expressed in fruits of the B+4 stage, in senescent leaves and in sepals. To further characterize its function, an RNA interference (RNAi) expression vector of SlMBP8 was constructed and transferred into tomato. In the transgenic plants, the ripening of fruits was shortened by 2-4 days compared to that of wild type...
September 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28626940/overexpression-of-hevea-brasiliensis-ethylene-response-factor-hberf-ixc5-enhances-growth-and-tolerance-to-abiotic-stress-and-affects-laticifer-differentiation
#18
Retno Lestari, Maryannick Rio, Florence Martin, Julie Leclercq, Natthakorn Woraathasin, Sandrine Roques, Florence Dessailly, Anne Clément-Vidal, Christine Sanier, Denis Fabre, Sémi Melliti, Sony Suharsono, Pascal Montoro
Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting stress for better latex production. This study sets out to establish and characterize rubber transgenic lines overexpressing HbERF-IXc5. Overexpression of HbERF-IXc5 dramatically enhanced plant growth and enabled plants to maintain some ecophysiological parameters in response to abiotic stress such as water deficit, cold and salt treatments...
June 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28620159/main-and-epistatic-loci-studies-in-soybean-for-sclerotinia-sclerotiorum-resistance-reveal-multiple-modes-of-resistance-in-multi-environments
#19
Tara C Moellers, Arti Singh, Jiaoping Zhang, Jae Brungardt, Mehdi Kabbage, Daren S Mueller, Craig R Grau, Ashish Ranjan, Damon L Smith, R V Chowda-Reddy, Asheesh K Singh
Genome-wide association (GWAS) and epistatic (GWES) studies along with expression studies in soybean [Glycine max (L.) Merr.] were leveraged to dissect the genetics of Sclerotinia stem rot (SSR) [caused by Sclerotinia sclerotiorum (Lib.) de Bary], a significant fungal disease causing yield and quality losses. A large association panel of 466 diverse plant introduction accessions were phenotyped in multiple field and controlled environments to: (1) discover sources of resistance, (2) identify SNPs associated with resistance, and (3) determine putative candidate genes to elucidate the mode of resistance...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28576486/construction-of-an-inducible-stable-cell-line-for-efficient-incorporation-of-unnatural-amino-acids-in-mammalian-cells
#20
Ziwei Zhang, Huan Xu, Longlong Si, Yi Chen, Bo Zhang, Yan Wang, Yiming Wu, Xueying Zhou, Lihe Zhang, Demin Zhou
The genetic incorporation of unnatural amino acids (Uaas) with defined properties into proteins at designated sites represents an extremely powerful tool for protein engineering. However, the efficient incorporation of Uaas in response to the amber stop codon in mammalian cells remains a substantial challenge due to the competition from release factor 1(RF1). Addressing this challenge will greatly broaden the power and scope of this technology. Here, we chose the eRF1 mutant, which can selectively enhance Uaa incorporation in response to the amber codon without increasing the readthrough of the opal and ochre codons...
August 5, 2017: Biochemical and Biophysical Research Communications
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