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https://www.readbyqxmd.com/read/28214223/evaluation-of-a-field-test-for-antibodies-against-chlamydia-trachomatis-during-trachoma-surveillance-in-nepal
#1
Michelle J Sun, Andrea I Zambrano, Laura Dize, Beatriz Munoz, Sarah Gwyn, Sailesh Mishra, Diana L Martin, Shekhar Sharma, Sheila K West
PURPOSE: Testing for antibodies to Chlamydia trachomatis has potential as a surveillance tool. Our evaluation compares lateral flow assays (LFAs) during surveillance surveys in Nepal with Multiplex bead array (MBA). Fifty children were randomly sampled from each of 15 random clusters in two districts of Nepal. Finger prick blood samples were collected from 1509 children and tested onsite for anti-Pgp3 antibodies by LFA. The LFA was read at 30min as negative, positive, or invalid. Tests results were also rated as difficult to read ("equivocal")...
January 17, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28213403/temporal-dissociation-between-minimal-distal-to-aortic-pressure-ratio-and-peak-hyperemia-during-intravenous-adenosine-infusion
#2
Lorena Casadonte, Koen M Marques, Jos Ae Spaan, Maria Siebes
The present study sought to compare the temporal relation between maximal coronary flow (peak hyperemia) and minimal coronary/aortic (Pd/Pa) pressure ratio for intracoronary (IC) and intravenous (IV) adenosine administration. Peak hyperemia is assumed to coincide with the minimal Pd/Pa value. However, this has not been confirmed for systemic hemodynamic variations during IV adenosine infusion. Hemodynamic responses to IV and IC adenosine administration were obtained in 12 patients (14 lesions) using combined intracoronary pressure and flow velocity measurements...
February 17, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28213280/magnetic-lateral-flow-immunoassay-test-strip-development-considerations-for-proof-of-concept-evaluation
#3
R Connolly, R O' Kennedy
Lateral flow immunoassays (LFIA) have grown to become the predominant test device format for the diagnostics and point-of-care industries. The demand for robust and reproducible LFIAs has been facilitated through scale-up production methods using specialized and automated instruments. However, the feasibility of a LFIA device can still be evaluated in a small-scale laboratory setting through controlled manual preparation methods. The advent of super-paramagnetic (SPMP) labels for use in lateral flow has heralded the possibility of highly sensitive and stable LFIAs...
February 14, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28209740/quantitative-mri-volumetry-diffusivity-cerebrovascular-flow-and-cranial-hydrodynamics-during-head-down-tilt-and-hypercapnia-the-spacecot-study
#4
Larry Allen Kramer, Khader M Hasan, Ashot E Sargsyan, Karina Marshall-Goebel, Jörn Rittweger, Dorit Donoviel, Saki Higashi, Benson Mwangi, Darius A Gerlach, Eric M Bershad
PURPOSE: In order to improve the pathophysiologic understanding of visual changes observed in astronauts, we aimed to use quantitative MRI to measure anatomic and physiologic responses during a ground-based spaceflight analogue (head down tilt, HDT) combined with increased ambient carbon dioxide (CO2). METHODS: Six healthy, male subjects participated in the double-blinded, randomized cross-over design study with two conditions: 26.5 h of -12° HDT with ambient air and with 0...
February 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28205436/comparison-of-lateral-flow-assay-kidney-inhibition-swab-and-liquid-chromatography-tandem-mass-spectrometry-for-the-detection-of-penicillin-g-residues-in-sow-urine
#5
Weilin L Shelver, Shubhashis Chakrabarty, David J Smith
Sows (n = 126) were administered penicillin G; urine, collected at slaughter, was screened by kidney inhibition swab (KIS; 4 h testing time) and then stored at -80 °C (∼1200 days) until analysis by lateral flow assay (LF, ∼5 min testing time) and tandem quadrupole LC-MS/MS (TQ) analysis. The stability of penicillin in urine during storage was verified using TQ analyses. Quantitative results were well-correlated (R(2) = 0.98) with only a ∼10% decrease in penicillin concentration during the 3-year storage period...
February 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28205389/growth-inhibition-of-formed-corneal-neovascularization-following-fosaprepitant-treatment
#6
Fabio Bignami, Anna Lorusso, Paolo Rama, Giulio Ferrari
PURPOSE: The aim of this study was to test the efficacy of Neurokinin-1 Receptor (NK-1R) antagonist -Fosaprepitant- in inducing regression of established corneal neovascularization (CNV). METHODS: Twenty C57BL/6 mice underwent alkali burn. Seven days later, when corneal neovessels had developed, they received Fosaprepitant 10 mg/ml, administered topically six times a day in the right eye for 10 days. In parallel, a group of 20 causticated mice was treated with normal saline, as control...
February 15, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28203194/accidental-ingestion-of-nasal-packing-gauze-during-endonasal-endoscopic-dacryocystorhinostomy-under-local-anesthesia-a-case-report
#7
Yoshiyuki Kitaguchi, Jacqueline Mupas-Uy, Yasuhiro Takahashi, Kazushige Ishida, Hirohiko Kakizaki
PURPOSE: To report a case of accidental ingestion of a nasal packing gauze during endonasal endoscopic dacryocystorhinostomy (en-DCR) under local anesthesia. CASE REPORT: A 66-year-old female patient underwent an en-DCR for a right acquired nasolacrimal duct obstruction. The surgery was performed in a supine position under local anesthesia. An X-ray detectable ribbon gauze soaked in 0.02% epinephrine was placed in the middle meatus to prevent blood and liquid from flowing into the pharynx...
January 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28203077/biosensing-of-dna-oxidative-damage-a-model-of-using-glucose-meter-for-non-glucose-biomarker-detection
#8
Xuena Zhu, Mehenur Sarwar, Qiaoli Yue, Chunying Chen, Chen-Zhong Li
Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL(-1) with a dynamic range of 1-200 ng mL(-1)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28197602/automating-multi-step-paper-based-assays-using-integrated-layering-of-reagents
#9
Sana Jahanshahi-Anbuhi, Balamurali Kannan, Kevin Pennings, M Monsur Ali, Vincent Leung, Karen Giang, Jingyun Wang, Dawn White, Yingfu Li, Robert H Pelton, John D Brennan, Carlos D M Filipe
We describe a versatile and simple method to perform sequential reactions on paper analytical devices by stacking dry pullulan films on paper, where each film contains one or more reagents or acts as a delay layer. Exposing the films to an aqueous solution of the analyte leads to sequential dissolution of the films in a temporally controlled manner followed by diffusive mixing of the reagents, so that sequential reactions can be performed. The films can be easily arranged for lateral flow assays or for spot tests (reactions take place sequentially in the z-direction)...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194991/simultaneous-detection-of-dual-nucleic-acids-using-a-sers-based-lateral-flow-assay-biosensor
#10
Xiaokun Wang, Namhyun Choi, Ziyi Cheng, Juhui Ko, Lingxin Chen, Jaebum Choo
A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28194732/late-post-avr-progression-of-bicuspid-aortopathy-link-to-hemodynamics
#11
Shiho Naito, Tatiana Gross, Kushtrim Disha, Yskert von Kodolitsch, Hermann Reichenspurner, Evaldas Girdauskas
BACKGROUND AND AIM OF THE STUDY: The ascending aortic dilatation may progress after aortic valve replacement (AVR) in bicuspid aortic valve (BAV) patients. Our aim was to evaluate rheological flow patterns and histological characteristics of the aneurysmal aorta in BAV patients at the time of reoperative aortic surgery. MATERIALS AND METHODS: 13 patients (mean age: 42 ± 9 years, 10 (77%) male) with significant progression of proximal aortopathy after isolated AVR surgery for BAV disease (i...
February 13, 2017: General Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28194062/performance-of-galactomannan-antigen-beta-d-glucan-and-aspergillus-lateral-flow-device-for-the-diagnosis-of-invasive-aspergillosis
#12
Gökhan Metan, Muzaffer Keklik, Gökçen Dinç, Çiğdem Pala, Afra Yıldırım, Berkay Saraymen, Mustafa Yavuz Köker, Leylagül Kaynar, Bülent Eser, Mustafa Çetin
Aspergillus lateral-flow device (LFD) was recently introduced as a practical tool for the diagnosis of invasive aspergillosis (IA). We investigated the performance of Aspergillus-LFD as a point-of-care test for the diagnosis of IA. Serum samples were collected twice weekly from patients who received intensive chemotherapy for acute leukemia, or recepients of allogeneic stem cell transplantation. Aspergillus galactomannan (GM) antigen, 1,3-beta-d-glucan and Aspergillus-LFD tests were carried out according to manufacturers' recommendations...
March 2017: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/28193020/viscoelastic-separation-of-particles-by-size-in-straight-rectangular-microchannels-a-parametric-study-for-a-refined-understanding
#13
Di Li, Xinyu Lu, Xiangchun Xuan
Microfluidic separation of particles has been implemented using a variety of force fields. We demonstrate in this work a continuous sheath-free separation of both a binary and a ternary particle mixture in viscoelastic polymer solutions through straight rectangular microchannels. This label-free separation arises from the flow-induced lift force that directs particles toward size-sensitive focusing positions in a high width/height channel. It is found to be a strong function of multiple experimental parameters, which is systematically investigated in terms of dimensionless numbers...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193014/simple-and-highly-sensitive-molecular-diagnosis-of-zika-virus-by-lateral-flow-assays
#14
Dohwan Lee, Yong Shin, Seok Chung, Kyo Seon Hwang, Dae Sung Yoon, Jeong Hoon Lee
We have developed a simple, user-friendly, and highly sensitive Zika virus (ZIKV) detection method by incorporating optimized reverse transcription loop-mediated isothermal amplification (RT-LAMP) and a lateral flow assay (LFA). The optimized RT-LAMP reaction was carried out using Bst 3.0 polymerase, which has robust and fast isothermal amplification performance even in the presence of high concentrations of inhibitors; this permitted the amplification of ZIKV RNA in pure water and human whole blood. In addition, the strong reverse transcription activity of Bst 3...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28192264/balloon-angioplasty-for-in-stent-restenosis-resulting-in-carotid-stent-fracture-with-literature-review-of-stent-in-stent-technique-as-a-viable-therapeutic-option
#15
Abdul-Haseeb Naeem, Ghaya Al-Rumaihi, Amirpouyan Namavarian, Manas Sharma, Melfort Boulton
BACKGROUND: Carotid stent fractures are rare and multiple etiologies have been proposed to explain their occurrence. We present a patient with a internal carotid artery (ICA) stent who developed in-stent restenosis. We performed balloon angioplasty to address in-stent restenosis but post-procedure he developed a carotid stent fracture. To our knowledge, balloon angioplasty has not been reported to cause stent fractures and we report the first case report. CASE DESCRIPTION: 72-year-old male underwent stenting for symptomatic left ICA stenosis with post-stenting residual stenosis of 55%...
February 9, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28192054/evaluation-of-immunoassays-and-general-biological-indicator-tests-for-field-screening-of-bacillus-anthracis-and-ricin
#16
Rachel A Bartholomew, Richard M Ozanich, Jennifer S Arce, Heather E Engelmann, Alejandro Heredia-Langner, Beth A Hofstad, Janine R Hutchison, Kristin Jarman, Angela M Melville, Kristin D Victry, Cynthia J Bruckner-Lea
There is little published data on the performance of biological indicator tests and immunoassays that could be used by first responders to determine if a suspicious powder contains a potential biothreat agent. We evaluated a range of biological indicator tests, including 3 protein tests, 2 ATP tests, 1 DNA test, and 1 FTIR spectroscopy instrument for their ability to screen suspicious powders for Bacillus anthracis (B. anthracis) spores and ricin. We also evaluated 12 immunoassays (mostly lateral flow immunoassays) for their ability to screen for B...
January 2017: Health Security
https://www.readbyqxmd.com/read/28191682/aortic-root-thrombus-complicated-by-left-main-coronary-artery-occlusion-visualized-by-3d-echocardiography-in-a-patient-with-continuous-flow-left-ventricular-assist-device
#17
Monique S Tanna, Alex Reyentovich, Leora B Balsam, John A Dodson, Alan F Vainrib, Ricardo J Benenstein, Barry P Rosenzweig, Muhamed Saric
Aortic root thrombus is an uncommon complication of continuous-flow left ventricular assist devices (LVAD). We present the case of a 71-year-old man with ischemic cardiomyopathy who underwent destination therapy HeartMate II LVAD placement. Eighteen months later, he presented with a cerebrovascular accident followed by myocardial infarction. Transesophageal echocardiography revealed an aortic root thrombus spanning the left and noncoronary cusps and obliterating the left main coronary artery. We discuss the incidence, risk factors, and management of aortic root thrombus in LVAD patients...
February 13, 2017: Echocardiography
https://www.readbyqxmd.com/read/28190730/leftward-flow-determines-laterality-in-conjoined-twins
#18
Matthias Tisler, Thomas Thumberger, Isabelle Schneider, Axel Schweickert, Martin Blum
Conjoined twins fused at the thorax display an enigmatic left-right defect: although left twins are normal, laterality is disturbed in one-half of right twins [1-3]. Molecularly, this randomization corresponds to a lack of asymmetric Nodal cascade induction in right twins [4]. We studied leftward flow [5, 6] at the left-right organizer (LRO) [7, 8] in thoracopagus twins in Xenopus, which displayed a duplicated, fused, and ciliated LRO. Cilia were motile and produced a leftward flow from the right LRO margin of the right to the left margin of the left twin...
February 7, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28190607/patient-specific-assessment-of-hemodynamics-by-computational-fluid-dynamics-in-patients-with-bicuspid-aortopathy
#19
Naoyuki Kimura, Masanori Nakamura, Kenji Komiya, Satoshi Nishi, Atsushi Yamaguchi, Osamu Tanaka, Yoshio Misawa, Hideo Adachi, Koji Kawahito
OBJECTIVE: Hemodynamics related to eccentric blood flow may factor into the development of bicuspid aortic valve aortopathy. We investigated wall shear stress distribution by means of magnetic resonance imaging-based computational fluid dynamics in patients with a bicuspid aortic valve. METHODS: Included were 12 patients with a bicuspid aortic valve (aortic stenosis, n = 11; root enlargement, n = 1). Three patients with a normal tricuspid aortic valve (arch aneurysm, n = 1; descending aortic aneurysm, n = 2) were included for comparison...
January 11, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28190501/middle-east-respiratory-syndrome-mers-coronavirus-and-dromedaries
#20
REVIEW
Ulrich Wernery, Susanna K P Lau, Patrick C Y Woo
Middle East Respiratory Syndrome (MERS) is a zoonotic viral disease that can be transmitted from dromedaries to human beings. More than 1500 cases of MERS have been reported in human beings to date. Although MERS has been associated with 30% case fatality in human beings, MERS coronavirus (MERS-CoV) infection in dromedaries is usually asymptomatic. In rare cases, dromedaries may develop mild respiratory signs. No MERS-CoV or antibodies against the virus have been detected in camelids other than dromedaries...
February 2017: Veterinary Journal
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