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https://www.readbyqxmd.com/read/28817769/a-paper-based-surface-enhanced-raman-scattering-lateral-flow-strip-for-detection-of-neuron-specific-enolase-in-blood-plasma
#1
Xuefei Gao, Peng Zheng, Sujan Kasani, Steven Wu, Feng Yang, Sara Lewis, Sara Nayeem, Elizabeth Engler-Chiurazzi, Jane Wigginton, James W Simpkins, Nianqiang Wu
An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to high sensitivity of device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix...
August 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28814081/monitoring-genetic-population-biomarkers-with-wastewater-based-epidemiology
#2
Zhugen Yang, Gaolian Xu, Julien Reboud, Barbara Kasprzyk-Hordern, Jonathan Mark Cooper
We report a rapid "sample-to-answer" platform for the quantitative monitoring of genetic biomarkers within communities by analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) and shows for the first time the ability to rapidly quantify human-specific mitochondrial DNA (mtDNA) from raw untreated wastewater samples. mtDNA provides a model population biomarker associated with carcinogenesis including breast, renal and gastric cancers. We integrated a filter to remove solid impurities and perform DNA extraction and enrichment in a low cost lateral flow-based test...
August 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28813601/platinum-decorated-gold-nanoparticles-with-dual-functionalities-for-ultrasensitive-colorimetric-in-vitro-diagnostics
#3
Zhuangqiang Gao, Haihang Ye, Dianyong Tang, Jing Tao, Sanaz Habibi, Adriene Minerick, Dianping Tang, Xiaohu Xia
Au nanoparticles (AuNPs) as signal reporters have been utilized in colorimetric in vitro diagnostics (IVDs) for decades. Nevertheless, it remains a grand challenge to substantially enhance the detection sensitivity of AuNP-based IVDs as confined by the inherent plasmonics of AuNPs. In this work, we circumvent this confinement by developing a unique dual-functional AuNPs that were engineered by coating conventional AuNPs with ultrathin Pt skins of sub-10 atomic layers (i.e., Au@Pt NPs). The Au@Pt NPs retain the plasmonic activity of initial AuNPs, while possess ultrahigh catalytic activity enabled by Pt skins...
August 16, 2017: Nano Letters
https://www.readbyqxmd.com/read/28810961/resuscitation-of-hypotensive-traumatic-brain-injured-animals-with-spray-dried-plasma-does-not-adversely-alter-physiology-and-improves-blood-brain-barrier-function
#4
Steven McDaniel, Stephanie Golla, Anthony N Moore, Joe DaCorta, Arthur Bode, Shibani Pati, Pramod K Dash, Jing Zhao
INTRODUCTION: According to the Defense and Veterans Brain Injury Center and the Armed Forces Health Surveillance Center, the number of soldiers who have sustained a traumatic brain injury (TBI) has risen dramatically over the past decade. Studies have shown that brain damage can be exacerbated if blood loss occurs (often occurring in polytrauma). As blood supply is critical for brain function and survival, TBI patients must be properly resuscitated to maintain blood volume, blood pressure, and cerebral perfusion...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28810259/reliability-reproducibility-and-prognostic-accuracy-of-the-alberta-stroke-program-early-ct-score-on-ct-perfusion-and-non-contrast-ct-in-hyperacute-stroke
#5
Jillian Naylor, Leonid Churilov, Ziyuan Chen, Miriam Koome, Neil Rane, Bruce C V Campbell
BACKGROUND: Alberta Stroke Program Early CT Score (ASPECTS) assesses early ischemic change on non-contrast CT (NCCT). We hypothesised that assessing ASPECTS regions on CT Perfusion (CTP) rather than NCCT would improve inter-rater agreement and prognostic accuracy, particularly in patients presenting early after stroke onset. METHODS: Ischemic stroke patients treated with intravenous alteplase from 2009 to 2014 at our institution were included in this study. Inter-rater agreement and prognostic accuracy of ASPECTS across modalities were analysed by the time between stroke onset and initial NCCT, dichotomized 1st quartile versus quartiles 2-4, referred to as epochs...
August 16, 2017: Cerebrovascular Diseases
https://www.readbyqxmd.com/read/28810037/quantitative-features-of-the-choriocapillaris-in-healthy-individuals-using-swept-source-optical-coherence-tomography-angiography
#6
Mayss Al-Sheikh, Khalil Ghasemi Falavarjani, Maximilian Pfau, Akihito Uji, Phillip P Le, SriniVas R Sadda
BACKGROUND AND OBJECTIVE: To quantify vessel density (VD) and grey value (GV) as a measure of flow in the choriocapillaris (CC) in healthy subjects with optical coherence tomography angiography (OCTA). PATIENTS AND METHODS: In this prospective, noncomparative case series, 3 mm × 3 mm OCTA images of 36 eyes of 22 healthy individuals were obtained using a swept-source instrument. VD and GV levels were calculated on CC en face slabs in the central 1-mm (subfoveal field) and surrounding 2...
August 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28808562/floral-scent-and-species-divergence-in-a-pair-of-sexually-deceptive-orchids
#7
Daniel D L Gervasi, Marc-Andre Selosse, Mathieu Sauve, Wittko Francke, Nicolas J Vereecken, Salvatore Cozzolino, Florian P Schiestl
Speciation is typically accompanied by the formation of isolation barriers between lineages. Commonly, reproductive barriers are separated into pre- and post-zygotic mechanisms that can evolve with different speed. In this study, we measured the strength of different reproductive barriers in two closely related, sympatric orchids of the Ophrys insectifera group, namely Ophrys insectifera and Ophrys aymoninii to infer possible mechanisms of speciation. We quantified pre- and post-pollination barriers through observation of pollen flow, by performing artificial inter- and intraspecific crosses and analyzing scent bouquets...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28807294/recombinase-polymerase-amplification-rpa-combined-with-lateral-flow-lf-strip-for-detection-of-toxoplasma-gondii-in-the-environment
#8
Y D Wu, M J Xu, Q Q Wang, C X Zhou, M Wang, X Q Zhu, D H Zhou
Toxoplasma gondii infects all warm-blooded vertebrates, resulting in a great threat to human health and significant economic loss to the livestock industry. Ingestion of infectious oocysts of T. gondii from the environment is the major source of transmission. Detection of T. gondii oocysts by existing methods is laborious, time-consuming and expensive. The objective of the present study was to develop a recombinase polymerase amplification (RPA) method combined with a lateral flow (LF) strip for detection of T...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28804833/laboratory-testing-protocols-for-heparin-induced-thrombocytopenia-hit-testing
#9
Kun Kan Edwin Lau, Soma Mohammed, Leonardo Pasalic, Emmanuel J Favaloro
Heparin-induced thrombocytopenia (HIT) represents a significant high morbidity complication of heparin therapy. The clinicopathological diagnosis of HIT remains challenging for many reasons; thus, laboratory testing represents an important component of an accurate diagnosis. Although there are many assays available to assess HIT, these essentially fall into two categories-(a) immunological assays, and (b) functional assays. The current chapter presents protocols for several HIT assays, being those that are most commonly performed in laboratory practice and have the widest geographic distribution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28803065/flow-induced-adherens-junction-remodeling-driven-by-cytoskeletal-forces
#10
Deepika Verma, Vivek Bajpai, Nannan Ye, Mohammad M Maneshi, Deekshitha Jetta, Stelios T Andreadis, Frederick Sachs, Susan Z Hua
Adherens junctions (AJs) are a key structural component for tissue organization and function. Under fluid shear stress, AJs exhibit dynamic assembly/disassembly, but how shear stress couples to AJs is unclear. In MDCK cells we measured simultaneously the forces in cytoskeletal α-actinin and the density and length of AJs using a genetically coded optical force sensor, actinin-sstFRET, and fluorescently labeled E-cadherin (E-cad). We found that shear stress of 0.74dyn/cm(2) for 3hours significantly enhanced E-cad expression at cell-cell contacts and this phenomenon has two phases...
August 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28802587/pre-transplant-infusion-of-donor-derived-dendritic-cells-maintained-at-the-immature-stage-by-sinomenine-increases-splenic-foxp3-tregs-in-recipient-rats-after-renal-allotransplantation
#11
Lian Li, Zhigang Luo, Zhe Song, Liwen Zheng, Tuo Chen
OBJECTIVE: The immunosuppressive mechanism of sinomenine in organ allotransplantation was investigated, especially its effect of blocking dendritic cell (DC) maturation, which might influence the frequency of regulatory T cells (Tregs). METHODS: Bone marrow cells from male donor Wistar rats were induced to differentiate into DCs in vitro in the presence or absence of sinomenine, and characterized by flow cytometry. These two groups of DCs were respectively injected into male recipient Sprague-Dawley rats via the tail vein, at both high and low doses...
August 9, 2017: Transplant Immunology
https://www.readbyqxmd.com/read/28801079/re-raising-muscle-flaps-a-randomised-controlled-animal-study
#12
K Bradshaw, M Wagels
BACKGROUND: In the experience of our centre, 33% of reconstructed compound lower limb injuries will need an orthopaedic revision.(1) A flap may be re-raised through numerous methods, and historically, the approach of choice has been based on the principle of protecting the vascular pedicle rather than the inset. Our aim was to determine whether a marginal versus a split approach to re-raising inferred a higher risk of flap necrosis and whether more attention should be paid to protecting the inset of the flap, particularly at the distal portion...
July 18, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28798852/flow-reproducibility-of-whole-blood-and-other-bodily-fluids-in-simplified-no-reaction-lateral-flow-assay-devices
#13
H Li, D Han, M A Hegener, G M Pauletti, A J Steckl
The "no reaction" lateral flow assay (nrLFA) uses a simplified LFA structure with no conjugate pad and no stored reagents. In the nrLFA, the capillary-based transport time or distance is the key indicator, rather than the outcome of a biochemical reaction. Hence, the calibration and reproducibility of the nrLFA device are critical. The capillary flow properties of several membrane types (nitrocellulose, nylon, cellulose acetate, polyethersulfone, and polyvinylidene difluoride) are evaluated. Flow rate evaluations of MilliporeSigma Hi-Flow™ Plus (HF075, HF135 and HF180) nitrocellulose membranes on nrLFA are performed using bodily fluids (whole blood, blood plasma, and artificial sweat)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28798144/frontline-science-leishmania-mexicana-amastigotes-can-replicate-within-neutrophils
#14
Benjamin P Hurrell, Manon Beaumann, Sandrina Heyde, Ivo B Regli, Andreas J Müller, Fabienne Tacchini-Cottier
Cutaneous leishmaniasis is a neglected tropical disease, causing a spectrum of clinical manifestations varying from self-healing to unhealing lesions that may be very difficult to treat. Emerging evidence points to a detrimental role for neutrophils during the first hours following infection with many distinct Leishmania species (spp.) at a time when the parasite is in its nonreplicative promastigote form. Neutrophils have also been detected at later stages of infection in unhealing chronic cutaneous lesions...
August 10, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28797494/laboratory-diagnostics-for-fungal-infections-a-review-of-current-and-future-diagnostic-assays
#15
REVIEW
Poornima Ramanan, Nancy L Wengenack, Elitza S Theel
This article reviews the current diagnostic approaches, both serologic and molecular, for the detection of fungi associated with pulmonary disease. Classic serologic techniques, including immunodiffusion and complement fixation, both of which remain a cornerstone for fungal diagnostic testing, are reviewed and their performance characteristics presented. More recent advances in this field, including novel lateral-flow assays for fungal antigen detection, are also described. Molecular techniques for fungal identification both from culture and directly from patient specimens, including nucleic acid probes, mass spectrometry-based methods, nucleic acid amplification testing, and traditional and broad-range sequencing, are discussed and their performance evaluated...
September 2017: Clinics in Chest Medicine
https://www.readbyqxmd.com/read/28797318/single-laboratory-validation-of-the-neogen-qualitative-lateral-flow-immunoassay-for-the-detection-of-paralytic-shellfish-toxins-in-mussels-and-oysters
#16
Alison R Turnbull, Jessica Y C Tan, Sarah C Ugalde, Gustaaf M Hallegraeff, Katrina Campbell, D Tim Harwood, Juan José Dorantes-Aranda
Detection of paralytic shellfish toxins (PSTs) in bivalve shellfish by analytical methods is complicated and costly, requiring specific expertise and equipment. Following extensive blooms of Alexandrium tamarense Group 1 in Tasmania, Australia, an investigation was made into commercially available screening test kits suitable for use with the toxin profiles found in affected bivalves. The qualitative Neogen rapid test kit, with a modified protocol to convert gonyautoxins GTX1&4 and GTX2&3 into neosaxitoxin and saxitoxin (STX), respectively, with higher cross-reactivities, was the best fit-for-purpose...
August 10, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28796606/directional-transverse-oscillation-vector-flow-estimation
#17
Jorgen Arendt Jensen
A method for estimating vector velocities using transverse oscillation (TO) combined with directional beamforming is presented. In directional TO (DTO), a normal focused field is emitted and the received signals are beamformed in the lateral direction transverse to the ultrasound beam to increase the amount of data for vector velocity estimation. The approach is self-calibrating as the lateral oscillation period is estimated from the directional signal through a Fourier transform to yield quantitative velocity results over a large range of depths...
August 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28793922/comparison-of-novel-and-standard-diagnostic-tools-for-the-detection-of-schistosoma-mekongi-infection-in-lao-people-s-democratic-republic-and-cambodia
#18
Youthanavanh Vonghachack, Somphou Sayasone, Virak Khieu, Robert Bergquist, Govert J van Dam, Pytsje T Hoekstra, Paul L A M Corstjens, Beatrice Nickel, Hanspeter Marti, Jürg Utzinger, Sinuon Muth, Peter Odermatt
BACKGROUND: Given the restricted distribution of Schistosoma mekongi in one province in Lao People's Democratic Republic (Lao PDR) and two provinces in Cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. However, sensitive diagnostic tools will be required to determine whether elimination has been achieved. We compared several standard and novel diagnostic tools in S...
August 10, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28792278/astrocytic-endfoot-ca-2-correlates-with-parenchymal-vessel-responses-during-4-ap-induced-epilepsy-an-in%C3%A2-vivo-two-photon-lifetime-microscopy-study
#19
Cong Zhang, Maryam Tabatabaei, Samuel Bélanger, Hélène Girouard, Mohammad Moeini, Xuecong Lu, Frédéric Lesage
Neurovascular coupling (NVC) underlying the local increase in blood flow during neural activity forms the basis of functional brain imaging and is altered in epilepsy. Because astrocytic calcium (Ca(2+)) signaling is involved in NVC, this study investigates the role of this pathway in epilepsy. Here, we exploit 4-AP induced epileptic events to show that absolute Ca(2+) concentration in cortical astrocyte endfeet in vivo correlates with the diameter of precapillary arterioles during neural activity. We simultaneously monitored free Ca(2+) concentration in astrocytic endfeet with the Ca(2+)-sensitive indicator OGB-1 and diameter of adjacent arterioles in the somatosensory cortex of adult mice by two-photon fluorescence lifetime measurements following 4-AP injection...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28791468/functional-outcomes-of-endoscopic-arytenoid-abduction-lateropexy-for-unilateral-vocal-cord-paralysis-with-dyspnea
#20
Vera Matievics, Adam Bach, Balazs Sztano, Zsofia Bere, Zoltan Tobias, Paul F Castellanos, Andreas H Mueller, Laszló Rovo
In unilateral vocal cord paralysis (UVCP), hoarseness is usually the leading symptom; however, the diminished airway might lead to breathing problems as well, especially with exertion. The application of the classic resection glottis enlarging or medialization procedures might shift the breathing and/or the voice to a worse condition. The non-destructive endoscopic arytenoid abduction lateropexy (EAAL) might be a solution for this problem. The aim of our study was to analyze the phonatory and respiratory outcomes of this treatment concept...
August 8, 2017: European Archives of Oto-rhino-laryngology
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