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https://www.readbyqxmd.com/read/29148720/a-dual-signal-readout-nanosphere-for-rapid-point-of-care-detection-of-ebola-virus-glycoprotein
#1
Jiao Hu, Yong-Zhong Jiang, Ling-Ling Wu, Zhen Wu, Yuhai Bi, Gary Wong, Xiang-Guo Qiu, Jian-Jun Chen, Dai-Wen Pang, Zhi-Ling Zhang
Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of EBOV glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target as well as to act as the signal enhancer...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29142810/the-role-of-ivabradine-in-diastolic-heart-failure-with-preserved-ejection-fraction-a-doppler-echocardiographic-study
#2
Federico Cacciapuoti, Valerio Massimo Magro, Michele Caturano, Diana Lama, Fulvio Cacciapuoti
Background: Ivabradine (IVA) is effective in patients with coronary artery disease (CAD) or systolic heart failure in sinus rhythm. Its action consists in reducing heart rate (HR) and improving the time of left ventricular (LV) diastolic filling. The aim of this study was to evaluate the effects of IVA added to conventional therapy on patients with diastolic heart failure (DHF) and preserved ejection fraction (HFpEF). Methods: We evaluated 25 patients with DHF in the New York Heart Association (NYHA) Class II-III and sinus rhythm...
October 2017: Journal of Cardiovascular Echography
https://www.readbyqxmd.com/read/29141740/a-lateral-flow-rapid-test-for-human-toxocariasis-developed-using-three-toxocara-canis-recombinant-antigens
#3
Muhammad Hafiznur Yunus, Siti Naqiuyah Tan Farrizam, Izzati Zahidah Abdul Karim, Rahmah Noordin
Laboratory diagnosis of toxocariasis is still a challenge especially in developing endemic countries with polyparasitism. In this study, three Toxocara canis recombinant antigens, rTES-26, rTES-30, and rTES-120, were expressed and used to prepare lateral flow immunoglobulin G4 (IgG4) dipsticks. The concordance of the results of the rapid test (comprising three dipsticks) with a commercial IgG-enzyme-linked immunosorbent assay (ELISA) (Cypress Diagnostics, Belgium) was compared against the concordance of two other commercial IgG-ELISA kits (Bordier, Switzerland and NovaTec, Germany) with the Cypress kit...
November 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29141048/microcirculation-in-open-vs-minimally-invasive-dorsal-stabilization-of-thoracolumbar-fractures
#4
Bergita Ganse, Miguel Pishnamaz, Philipp Kobbe, Christian Herren, Gertraud Gradl-Dietsch, Franziska Böhle, Bernd Johannes, Bong-Sung Kim, Klemens Horst, Matthias Knobe
Standard open and percutaneous minimally invasive surgical procedures co-exist in the treatment of fractures of the thoracolumbar spine. Shorter skin incisions just above the pedicles are used in minimally invasive procedures. Full-length skin incisions and invasive preparations are applied in the standard open approach. While both methods show equivalent rates of intraoperative surgical complications and comparable clinical and radiological outcomes, blood loss and operation time have shown to be decreased in minimally invasive treatment...
2017: PloS One
https://www.readbyqxmd.com/read/29141043/lateral-migration-of-electrospun-hydrogel-nanofilaments-in-an-oscillatory-flow
#5
Sylwia Pawłowska, Paweł Nakielski, Filippo Pierini, Izabela K Piechocka, Krzysztof Zembrzycki, Tomasz A Kowalewski
The recent progress in bioengineering has created great interest in the dynamics and manipulation of long, deformable macromolecules interacting with fluid flow. We report experimental data on the cross-flow migration, bending, and buckling of extremely deformable hydrogel nanofilaments conveyed by an oscillatory flow into a microchannel. The changes in migration velocity and filament orientation are related to the flow velocity and the filament's initial position, deformation, and length. The observed migration dynamics of hydrogel filaments qualitatively confirms the validity of the previously developed worm-like bead-chain hydrodynamic model...
2017: PloS One
https://www.readbyqxmd.com/read/29138790/high-efficiency-single-cell-encapsulation-and-size-selective-capture-of-cells-in-picoliter-droplets-based-on-hydrodynamic-micro-vortices
#6
Gopakumar Kamalakshakurup, Abraham P Lee
Single cell analysis has emerged as a paradigm shift in cell biology to understand the heterogeneity of individual cells in a clone for pathological interrogation. Microfluidic droplet technology is a compelling platform to perform single cell analysis by encapsulating single cells inside picoliter-nanoliter (pL-nL) volume droplets. However, one of the primary challenges for droplet based single cell assays is single cell encapsulation in droplets, currently achieved either randomly, dictated by Poisson statistics, or by hydrodynamic techniques...
November 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29137710/loop-mediated-isothermal-amplification-using-self-avoiding-molecular-recognition-systems-and-antarctic-thermal-sensitive-uracil-dna-glycosylase-for-detection-of-nucleic-acid-with-prevention-of-carryover-contamination
#7
Yi Wang, Dongxin Liu, Jianping Deng, Yan Wang, Jianguo Xu, Changyun Ye
Loop-mediated isothermal amplification (LAMP) is the most popular technique to amplify nucleic acid sequence without the use of temperature cycling. However, LAMP is often confounded by false-positive results, arising from interactions between (hetero-dimer) or within (self-dimerization) primers, off-target hybrids and carryover contaminants. Here, we devised a new LAMP technique that is self-avoiding molecular recognition system (SAMRS) components and antarctic thermal sensitive uracil-DNA-glycosylase (AUDG) enzyme-assisted, termed AUDG-SAMRS-LAMP...
December 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29136915/a-quantum-dot-based-lateral-flow-immunoassay-for-the-sensitive-detection-of-human-heart-fatty-acid-binding-protein-hfabp-in-human-serum
#8
Mihaela Savin, Carmen-Marinela Mihailescu, Iulia Matei, Dana Stan, Carmen Aura Moldovan, Marian Ion, Ion Baciu
We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135409/double-inlay-plus-ventral-onlay-buccal-mucosa-graft-for-simultaneous-penile-and-bulbar-urethral-stricture
#9
Luciano A Favorito, Paulo P Conte, Ulisses G Sobrinho, Rodrigo G Martins, Tomas Accioly
OBJECTIVES: Buccal mucosa grafts and fascio-cutaneous flaps are frequently used in long anterior urethral strictures (1). The inlay and onlay buccal mucosa grafts are easier to perform, do not need urethral mobilization and generally have good long-term results (2-4). In the present video, we present a case where we used a double buccal mucosa graft technique in a simultaneous penile and bulbar urethral stricture. MATERIALS AND METHODS: A 54 year-old male patient was submitted to appendectomy where a urethral catheter was used for two days in May 2015...
November 17, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/29133362/three-month-endothelial-human-endothelin-1-overexpression-causes-blood-pressure-elevation-and-vascular-and-kidney-injury
#10
Suellen C Coelho, Olga Berillo, Antoine Caillon, Sofiane Ouerd, Júlio C Fraulob-Aquino, Tlili Barhoumi, Stefan Offermanns, Pierre Paradis, Ernesto L Schiffrin
Endothelium-derived endothelin (ET)-1 has been implicated in the development of hypertension and end-organ damage, but its exact role remains unclear. We have shown that tamoxifen-inducible endothelium-restricted human ET-1 overexpressing (ieET-1) mice exhibited blood pressure rise after a 3-week induction in an ET type A (ETA) receptor-dependent manner, in absence of vascular and renal injury. It is unknown whether long-term ET-1 overexpression results in sustained blood pressure elevation and vascular and renal injury...
November 13, 2017: Hypertension
https://www.readbyqxmd.com/read/29133151/laparoscopic-management-of-a-rudimentary-uterine-horn
#11
Emilie Faller, Jean Jacques Baldauf, François Becmeur, Anne Lehn, Cherif Youssef Akladios, Lise Lecointre
STUDY OBJECTIVE: To demonstrate a technique of laparoscopic management of a rudimentary horn in a 12-year-old girl. DESIGN: A step-by-step explanation of the surgery using video (instructive video), approved the local institutional Review Board. SETTING: A university hospital, University Hospital of Strasbourg, France. PATIENT: A 12-year-old girl with a uterine rudimentary horn. INTERVENTION: We describe the case of a 12 years old girl who had no medical history...
November 10, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29133005/correlation-between-contrast-time-density-time-on-digital-subtraction-angiography-and-flow-an-in-vitro-study
#12
Denise Brunozzi, Sophia F Shakur, Rahim Ismail, Andreas Linninger, Chih-Yang Hsu, Fady T Charbel, Ali Alaraj
BACKGROUND: and Purpose Digital subtraction angiography (DSA) provides an excellent anatomic characterization of cerebral vasculature, but hemodynamic assessment is often qualitative and subjective. Various clinical algorithms have been produced to semi-quantify flow from data obtained from DSA, but few have tested these against reliable flow values. MATERIALS AND METHODS: An arched flow model was created and injected with contrast. 17 injections were acquired in anterior-posterior and lateral DSA projections, 4 injections were acquired in oblique projection...
November 10, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29129151/false-positive-diagnosis-of-brain-death-following-the-pediatric-guidelines-case-report-and-discussion
#13
D Alan Shewmon
A 2-year-old boy with severe head trauma was diagnosed brain dead according to the 2011 Pediatric Guidelines. Computed tomographic (CT) scan showed massive cerebral edema with herniation. Intracranial pressures were extremely high, with cerebral perfusion pressures around 0 for several hours. An apnea test was initially contraindicated; later, one had to be terminated due to oxygen desaturation when the Pco2 had risen to 57.9 mm Hg. An electroencephalogram (EEG) was probably isoelectric but formally interpreted as equivocal...
December 2017: Journal of Child Neurology
https://www.readbyqxmd.com/read/29125848/early-decreased-tlr2-expression-on-monocytes-is-associated-with-their-reduced-phagocytic-activity-and-impaired-maturation-in-a-porcine-polytrauma-model
#14
Lukas Schimunek, Rafael Serve, Michel P J Teuben, Philipp Störmann, Birgit Auner, Mathias Woschek, Roman Pfeifer, Klemens Horst, Tim-P Simon, Miriam Kalbitz, Ramona Sturm, Hans-C Pape, Frank Hildebrand, Ingo Marzi, Borna Relja
In their post-traumatic course, trauma patients suffering from multiple injuries have a high risk for immune dysregulation, which may contribute to post-injury complications and late mortality. Monocytes as specific effector cells of the innate immunity play a crucial role in inflammation. Using their Pattern Recognition Receptors (PRRs), notably Toll-Like Receptors (TLR), the monocytes recognize pathogens and/or pathogen-associated molecular patterns (PAMPs) and organize their clearance. TLR2 is the major receptor for particles of gram-positive bacteria, and initiates their phagocytosis...
2017: PloS One
https://www.readbyqxmd.com/read/29124533/syngeneic-and-xenogeneic-transplantations-of-mesenchymal-stromal-cells-modify-the-production-of-reactive-oxygen-species-by-blood-mononuclears-of-mice
#15
E V Agaeva, V N Petrov, A G Konoplyannikov, O E Popovkina, L A Lepekhina, E V Sayapina, I V Semenkova
In vivo modifying effects of bone marrow mesenchymal stromal cells of humans and laboratory mice on ROS production by mouse blood mononuclears are studied by luminol-dependent zymosan-induced chemiluminescence after syngeneic and xenogeneic transplantation into systemic blood flow. The chemiluminescent activity of mouse blood mononuclears has increased early (1 day) after syngeneic (mouse mesenchymal stromal cells) and xenogeneic (human mesenchymal stromal cells) transplantation. Later, 7-21 days after syngeneic and xenogeneic transplantation, the chemiluminescent activity of mouse mononuclears is suppressed...
November 9, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29123099/spatio-temporally-separated-cortical-flows-and-spindle-geometry-establish-physical-asymmetry-in-fly-neural-stem-cells
#16
Chantal Roubinet, Anna Tsankova, Tri Thanh Pham, Arnaud Monnard, Emmanuel Caussinus, Markus Affolter, Clemens Cabernard
Asymmetric cell division, creating sibling cells with distinct developmental potentials, can be manifested in sibling cell size asymmetry. This form of physical asymmetry occurs in several metazoan cells, but the underlying mechanisms and function are incompletely understood. Here we use Drosophila neural stem cells to elucidate the mechanisms involved in physical asymmetry establishment. We show that Myosin relocalizes to the cleavage furrow via two distinct cortical Myosin flows: at anaphase onset, a polarity induced, basally directed Myosin flow clears Myosin from the apical cortex...
November 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/29122653/validation-of-an-automated-bilateral-feet-cold-pressor-test
#17
Petra Bachmann, Xinwei Zhang, Mauro F Larra, Dagmar Rebeck, Karsten Schönbein, Klaus P Koch, Hartmut Schächinger
The Cold Pressor Test (CPT) is often used in psychobiological research. However, the classical CPT version (unilateral hand immersion into ice-water) involves some disadvantages: hands may be needed for further applications, attentional drift towards the affected sensory hemi-field and/or physiological activation of the contralateral hemisphere may produce a laterality bias. Furthermore, instruction-induced motor activity may bias physiologic reactivity. To avoid these problems, a fully automated bilateral feet CPT was developed and tested for validity and feasibility...
November 6, 2017: International Journal of Psychophysiology
https://www.readbyqxmd.com/read/29122551/crispr-mediated-deletion-of-the-pecam-1-cytoplasmic-domain-increases-receptor-lateral-mobility-and-strengthens-endothelial-cell-junctional-integrity
#18
Danying Liao, Heng Mei, Yu Hu, Debra K Newman, Peter J Newman
AIMS: PECAM-1 is an abundant endothelial cell surface receptor that becomes highly enriched at endothelial cell-cell junctions, where it functions to mediate leukocyte transendothelial migration, sense changes in shear and flow, and maintain the vascular permeability barrier. Homophilic interactions mediated by the PECAM-1 extracellular domain are known to be required for PECAM-1 to perform these functions; however, much less is understood about the role of its cytoplasmic domain in these processes...
November 6, 2017: Life Sciences
https://www.readbyqxmd.com/read/29121743/profiling-of-cardiovascular-and-cerebrovascular-disease-associated-microrna-expression-in-umbilical-cord-blood-in-gestational-hypertension-preeclampsia-and-fetal-growth-restriction
#19
Ilona Hromadnikova, Katerina Kotlabova, Katarina Ivankova, Yulia Vedmetskaya, Ladislav Krofta
BACKGROUND AND METHODS: Gene expression of 29 cardiovascular and cerebrovascular disease associated microRNAs was assessed in whole umbilical cord blood, compared between groups [47 gestational hypertension (GH), 56 preeclampsia (PE), 37 fetal growth restriction (FGR) and 44 normal pregnancies] and correlated with the severity of the disease with respect to clinical signs (mild PE vs. severe PE), delivery date (before and after 34weeks of gestation), and Doppler ultrasound parameters [pulsatility index (PI) in the umbilical artery, PI in the middle cerebral artery and the cerebroplacental ratio]...
December 15, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/29121579/a-process-oriented-hydro-biogeochemical-model-enabling-simulation-of-gaseous-carbon-and-nitrogen-emissions-and-hydrologic-nitrogen-losses-from-a-subtropical-catchment
#20
Wei Zhang, Yong Li, Bo Zhu, Xunhua Zheng, Chunyan Liu, Jialiang Tang, Fang Su, Chong Zhang, Xiaotang Ju, Jia Deng
Quantification of nitrogen losses and net greenhouse gas (GHG) emissions from catchments is essential for evaluating the sustainability of ecosystems. However, the hydrologic processes without lateral flows hinder the application of biogeochemical models to this challenging task. To solve this issue, we developed a coupled hydrological and biogeochemical model, Catchment Nutrients Management Model - DeNitrification-DeComposition Model (CNMM-DNDC), to include both vertical and lateral mass flows. By incorporating the core biogeochemical processes (including decomposition, nitrification, denitrification and fermentation) of the DNDC into the spatially distributed hydrologic framework of the CNMM, the simulation of lateral water flows and their influences on nitrogen transportation can be realized...
November 6, 2017: Science of the Total Environment
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