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Zebra fish

Frances E Clark, Matthew A Conte, Irani A Ferreira-Bravo, Andreia B Poletto, Cesar Martins, Thomas D Kocher
B chromosomes are extra chromosomes found in many species of plants, animals, and fungi. B chromosomes often manipulate common cellular processes to increase their frequency, sometimes to the detriment of organismal fitness. Here, we characterize B chromosomes in several species of Lake Malawi cichlid fish. Whole genome sequencing of Metriaclima zebra "Boadzulu" individuals revealed blocks of sequence with unusually high sequence coverage, indicative of increased copy number of those sequences. These regions of high sequence coverage were found only in females...
September 14, 2016: Journal of Heredity
Baolei Yuan, Xingshun Wang, Chunyan Fan, Jin You, Yuchu Liu, Jason D Weber, Hanbing Zhongand, Yandong Zhang
The RNA helicase DHX33 has been shown to be a critical regulator of cell proliferation and growth. However, the underlying mechanisms behind DHX33 function remain incompletely understood. We present original evidence in multiple cell lines that DHX33 transcriptionally controls the expression of genes involved in cell cycle, notably cyclins, E2F1, CDCs and MCMs. DHX33 physically associates with the promoters of these genes and controls the loading of active RNA polymerase II onto these promoters. DHX33 deficiency abrogates cell cycle progression, DNA replication, and leads to cell apoptosis...
September 6, 2016: Molecular and Cellular Biology
Qi Zhang, Decheng Wang, Guozhong Jiang, Wei Liu, Qing Deng, Xiujun Li, Wei Qian, Hugues Ouellet, Jianjun Sun
EsxA is required for virulence of Mycobacterium tuberculosis (Mtb) and plays an essential role in phagosome rupture and translocation to the cytosol of macrophages. Recent biochemical studies have demonstrated that EsxA is a membrane-permeabilizing protein. However, evidence that link EsxA membrane-permeabilizing activity to Mtb cytosolic translocation and virulence is lacking. Here we found that mutations at glutamine 5 (Q5) could up or down regulate EsxA membrane-permeabilizing activity. The mutation Q5K significantly diminished the membrane-permeabilizing activity, while Q5V enhanced the activity...
2016: Scientific Reports
Yang Yu, Feng Sun, Congcong Zhang, Zhendong Wang, Jinfeng Liu, Haining Tan
Soluble O-(2-hydroxyl)propyl-3-trimethyl ammonium chitooligosaccharide chloride (HTCOSC) was covalently conjugated to the 11-amino-acid peptide derived from amino terminus of endostatin (endostatin2, ES2, IVRRADRAAVP) to overcome its poor stability, low cell affinity and instable activity. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to study the structure and molecular weight information. The anti-angiogenic activities were evaluated using cell counting Kit-8 assay, flow cytometry assay, wounding migration assay, transwell migration assay, chicken chorioallantoic membrane (CAM) assay and zebra fish angiogenesis assay...
December 10, 2016: Carbohydrate Polymers
Jia Choi, Kui-Jin Kim, Byung-Hak Kim, Eun-Jeong Koh, Min-Jung Seo, Boo-Yong Lee
The present study was performed to investigate the molecular mechanism of 6-gingerol on adipocyte-mediated systemic inflammation in vitro and in high-fat diet-induced obese zebra fish. 6-Gingerol decreased adipogenesis due to the suppression of adipocyte differentiation markers, including peroxisome proliferator-activated receptor gamma, CCAATT enhancer binding protein α, and adipocyte protein 2, and triglyceride synthesis enzymes, including sterol regulatory element-binding protein-1, fatty acid synthase, lysophosphatidic acid acyltransferase, and acyl-coA : diacylglycerol acyltransferase 1, in 3T3-L1...
August 15, 2016: Planta Medica
Jun Cao, Yueqing Lv
Jacalin-related lectins are a type of carbohydrate-binding proteins, which are distributed across a wide variety of organisms and involved in some important biological processes. The evolution of this gene family in fishes is unknown. Here, 47 putative jacalin genes in 11 fish species were identified and divided into 4 groups through phylogenetic analysis. Conserved gene organization and motif distribution existed in each group, suggesting their functional conservation. Some fishes have eleven jacalin genes, while others have only one or zero gene in their genomes, suggesting dynamic changes in the number of jacalin genes during the evolution of fishes...
September 2016: Fish & Shellfish Immunology
Peter Kämpfer, Rute Irgang, Hans-Jürgen Busse, Mathías Poblete-Morales, Tanita Kleinhagauer, Stefanie P Glaeser, Ruben Avendaño-Herrera
One beige pigmented, Gram-staining negative, rod-shaped bacterium, strain E3/2T, was isolated from a Zebra fish, Danio rerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 97.7% 16S rRNA gene sequence identity to the species Pseudoduganella vioaceinigra and between 97.4 to 97.0% to some Duganella and Massilia species, including Duganella radicis, Duganella phyllosphaerae, Massilia dura, Massilia lutea, Duganella sacchari, Duganella zoogloeoides, Massilia albidiflava and Massilia umbonata...
August 8, 2016: International Journal of Systematic and Evolutionary Microbiology
Deng-Tai Wen, Lan Zheng, Liu Ni, Hui Wang, Yue Feng, Min Zhang
The CG9940 gene, which encodes the NAD(+) synthase protein in Drosophila, is conserved in human, zebra fish, and mosquito. NAD(+) synthase is a homodimer, which catalyzes the final step in de novo nicotinamide adenine dinucleotide (NAD(+)) biosynthesis, an amide transfer from either ammonia or glutamine to nicotinic acid adenine dinucleotide (NaAD). Both the CG9940 and exercise are closely relative to NAD(+) level, and NAD(+) plays important roles not only in energy metabolism and mitochondrial functions but also in aging...
October 2016: Experimental Gerontology
Jörg Bohlen, Vendula Šlechtová, Vlastimil Šlechta, Vera Šlechtová, Alexandr Sember, Petr Ráb
One of the most efficient mechanisms to keep animal lineages separate is a difference in ploidy level (number of whole genome copies), since hybrid offspring from parents with different ploidy level are functionally sterile. In the freshwater fish family Botiidae, ploidy difference has been held responsible for the separation of its two subfamilies, the evolutionary tetraploid Botiinae and the diploid Leptobotiinae. Diploid and tetraploid species coexist in the upper Yangtze, the Pearl River and the Red River basins in China...
2016: PloS One
Carla Bacchetta, Gerardo López, Gisela Pagano, Diana Tomazi Muratt, Leandro Machado de Carvalho, José María Monserrat
The antimicrobial activity of silver nanoparticles (AgNP) makes them useful in a wide range of products although their environmental impact is still uncertain. The main goal of this study was to evaluate short-term effects induced by AgNP on gills oxidative status and bacterial communities living at the skin mucus of zebrafish. Both the number of bacteria colony forming units and bacteria growth obtained from skin mucus were lower in all concentrations tested (25, 50 and 100 µg nAg/L). Besides, AgNP exposure caused a significant decrease in bacteria growth in zebrafish exposed to 100 µg nAg/L...
October 2016: Bulletin of Environmental Contamination and Toxicology
Xin Liu, Xing Huang, Zhiyun Xu, Deqin Yang
OBJECTIVE: To investigate the mechanism of retinoic acid (RA) signal in dental evolution, RA is used to explore the influence of the mechanism on neural crest's migration during the early stage of zebra fish embryos. METHODS: We divided embryos of wild type and transgenic line zebra fish into three groups. 1 x 10(-7) to 6 x 10(-7) mol x L(-1) RA and 1 x 10(-7) mo x L(-1) 4-diethylaminobenzaldehyde (DEAB) were added into egg water at 24 hpf for 9 h. Dimethyl sulfoxid (DMSO) with the concentration was used as control group...
April 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Roghieh Safari, Seyed Hossein Hoseinifar, Morteza Kavandi
The present study explores the effect of dietary sodium propionate on mucosal immune response and expression of antioxidant enzyme genes in zebra fish (Danio rerio). Six hundred healthy zebra fish (0.42 ± 0.06 g) supplied, randomly stocked in 12 aquariums and fed on basal diets supplemented with different levels of sodium propionate [0 (control), 5, 10 and 20 g kg(-1)] for 8 weeks. At the end of the feeding trial, mucosal immune parameters (TNF-α, IL-1β, Lyz), antioxidant enzyme (SOD, CAT) as well as heat shock protein 70 (HSP70) gene expression were measured...
June 18, 2016: Fish Physiology and Biochemistry
Peter Kämpfer, Rute Irgang, Hans-Jürgen Busse, Matías Poblete-Morales, Tanita Kleinhagauer, Stefanie P Glaeser, Ruben Avendaño-Herrera
One beige pigmented, Gram-staining negative, rod-shaped bacterium, strain E3/4T, was isolated from a Zebra fish, Danio rerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.5% 16S rRNA gene sequence identity to the type strain of Undibacterium macrobrachii and 97.8% to the type strain of Undibacterium seohonense. Lower 16S rRNA gene sequence similarities (< 97.0%) could be found in comparison to all other Undibacterium species. DNA-DNA hybridisation (DDH) with Undibacterium macrobrachii LMG 26891T showed low < 35%...
June 15, 2016: International Journal of Systematic and Evolutionary Microbiology
Venugopalan Rajesh, Sakthivel Ilanthalir
Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in several experimental paradigms. This study was undertaken to examine the effect of sulforaphane on cognitive impairment in zebra fish model using a novel method of fear conditioning. Initially, the normal behaviour of zebra fishes was studied in light-dark tank for 10 min daily for 10 days...
June 2, 2016: Neurochemical Research
Joel Cano-Nicolau, Clémentine Garoche, Nathalie Hinfray, Elisabeth Pellegrini, Noureddine Boujrad, Farzad Pakdel, Olivier Kah, François Brion
The effects of some progestins on fish reproduction have been recently reported revealing the hazard of this class of steroidal pharmaceuticals. However, their effects at the central nervous system level have been poorly studied until now. Notwithstanding, progesterone, although still widely considered primarily a sex hormone, is an important agent affecting many central nervous system functions. Herein, we investigated the effects of a large set of synthetic ligands of the nuclear progesterone receptor on the glial-specific expression of the zebrafish brain aromatase (cyp19a1b) using zebrafish mechanism-based assays...
August 15, 2016: Toxicology and Applied Pharmacology
S S Vutukuru, Jayasree Ganugapati, Vardhini Ganesh, P Atheeksha, Ravindra Babu Potti
Endocrine disrupting chemicals may induce adverse health effects in humans and wildlife. Recent studies demonstrate that endocrine disrupting chemicals like Bisphenol A (BPA), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ether (PBDE) affect the reproductive characters shared by wide range of creatures including fish. An attempt was made to evaluate the toxicity of these chemicals on the vitellogenin protein of zebra fish (Danio rerio) using in silico approach. The protein structure of zebra fish vitellogenin was predicted using homology modelling, and the stereochemical quality of the model was validated by Ramachandran plot...
May 30, 2016: Fish Physiology and Biochemistry
Man-Hong Keung, Lai-Sheung Chan, Hoi-Hin Kwok, Ricky Ngok-Shun Wong, Patrick Ying-Kit Yue
BACKGROUND: Ginsenoside-Rg3, the pharmacologically active component of red ginseng, has been found to inhibit tumor growth, invasion, metastasis, and angiogenesis in various cancer models. Previously, we found that 20(R)-ginsenoside-Rg3 (Rg3) could inhibit angiogenesis. Since microRNAs (miRNAs) have been shown to affect many biological processes, they might play an important role in ginsenoside-mediated angiomodulation. METHODS: In this study, we examined the underlying mechanisms of Rg3-induced angiosuppression through modulating the miRNA expression...
April 2016: Journal of Ginseng Research
Cristian Gutierrez-Ibanez, Andrew N Iwaniuk, Megan Jensen, David J Graham, Ákos Pogány, Benjamin C Mongomery, James L Stafford, Harald Luksh, Douglas R Wylie
Cocaine- and amphetamine-regulated transcript peptides (CARTp) are neuropeptides that acts as neurotransmitters in the brain of vertebrates. The expression of CARTp has been characterised in teleost, amphibians and several mammalian species, but comparative data in reptiles and birds is non-existant. In this study, we show the distribution of immunoreactivity against CART peptides (CARTp-ir) in the brains of the two bird species: the pigeon (Columba livia) and zebra finch (Taeniopygia guttata). We found CARTp-ir cells and terminals in the brains of both, but no major differences between the two species...
April 28, 2016: Journal of Comparative Neurology
Yi Yuan, Min Liu, Ru-juan Chen, Feng-hua Wei, Gui-lin Li, Jian-qiang Li, Xing-jian Xu
OBJECTIVE: To evaluate the acute toxicity of 10% LDS, a new molluscicide, to non-target organisms. METHODS: Based on "Chemical pesticide environmental safety test evaluation standard", an acute toxicity test was carried out with Coturnix coturnix japonica (quail), Apis mellifera L (bee), Bombyx mori (silkworm), and Brachydonio rerio (zebra fish) , and the skin stimulus test was also performed with guinea pig. RESULTS: The quails had no toxic symptoms while the maximum poisoning concentration of LDS was 200 mg/kg (no toxicity)...
December 2015: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
K Mohammed-Geba, F Arrutia, H Do-Huu, Y J Borrell, A Galal-Khallaf, A Ardura, Francisco A Riera, Eva Garcia-Vazquez
Today enormous research efforts are being focused on alleviating the massive, adverse effects of obesity. Short peptides are key targets for research as they can be generated from natural proteins, like milk. Here we conducted trypsinogen digestion of beta-lactoglobulin (β-lg), the major mammalian milk protein, to release the hexamer VY6. It was assayed in vivo for its activities on lipid metabolism using zebra fish as a vertebrate model. Zebra fish juveniles were injected with two different doses of the peptide: 100 and 800 μg per g fish and left for 5 days before sacrificing...
April 2016: Food & Function
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