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https://www.readbyqxmd.com/read/28806601/influence-of-sublethal-doses-of-acetamiprid-and-halosulfuron-methyl-on-metabolites-of-zebra-fish-brachydanio-rerio
#1
Hong Zhang, Lijuan Zhao
Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to investigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio)...
August 4, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28775072/lmo2-lim-domain-only-2-modulates-sphk1-sphingosine-kinase-and-promotes-endothelial-cell-migration
#2
Gianfranco Matrone, Shu Meng, Qilin Gu, Jie Lv, Longhou Fang, Kaifu Chen, John P Cooke
OBJECTIVE: Lmo (LIM-domain-only)2 transcription factor is involved in hematopoiesis and vascular remodeling. Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). We hypothesized that Lmo2 regulates Sphk1 to promote endothelial cell (EC) migration and vascular development. APPROACH AND RESULTS: Lmo2 and Sphk1 knockdown (KD) were performed in Tg(fli1:EGFP) (y1) zebra fish and in human umbilical vein EC. Rescue of phenotypes or overexpression of these factors were achieved using mRNA encoding Lmo2 or Sphk1...
August 3, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28774750/advances-in-nonhuman-primate-models-of-autism-integrating-neuroscience-and-behavior
#3
REVIEW
M D Bauman, C S Schumann
Given the prevalence and societal impact of autism spectrum disorders (ASD), there is an urgent need to develop innovative preventative strategies and treatments to reduce the alarming number of cases and improve core symptoms for afflicted individuals. Translational efforts between clinical and preclinical research are needed to (i) identify and evaluate putative causes of ASD, (ii) determine the underlying neurobiological mechanisms, (iii) develop and test novel therapeutic approaches and (iv) ultimately translate basic research into safe and effective clinical practices...
July 31, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28766927/combining-hyperspectral-imaging-and-chemometrics-to-assess-and-interpret-the-effects-of-environmental-stressors-on-the-organism-at-tissue-level
#4
Víctor Olmos, Mònica Marro, Pablo Loza-Alvarez, Demetrio Raldúa, Eva Prats, Francesc Padrós, Benjamin Piña, Romà Tauler, Anna de Juan
Changes on an organism by the exposure to environmental stressors may be characterized by hyperspectral images (HSI), which preserve the morphology of biological samples, and suitable chemometric tools. The approach proposed allows assessing and interpreting the effect of contaminant exposure on heterogeneous biological samples monitored by HSI at specific tissue levels. In this work, the model example used consists of the study of the effect of the exposure of chlorpyrifos-oxon on zebra fish tissues. To assess this effect, unmixing of the biological sample images followed by tissue-specific classification models based on the unmixed spectral signatures is proposed...
August 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28762049/biogenic-synthesis-of-gold-nanoparticles-from-terminalia-arjuna-bark-extract-assessment-of-safety-aspects-and-neuroprotective-potential-via-antioxidant-anticholinesterase-and-antiamyloidogenic-effects
#5
Natarajan Suganthy, Vijayan Sri Ramkumar, Arivalagan Pugazhendhi, Giovanni Benelli, Govindaraju Archunan
The development of neuroprotective drugs through eco-friendly production routes is a major challenge for current pharmacology. The present study was carried out to synthesize gold nanoparticles (AuNPs) through biogenic route using ethanolic bark extract of Terminalia arjuna, a plant of high interest in Asian traditional medicine, and to evaluate its neuroprotective effects. The synthesized AuNPs were characterized by UV-Vis spectroscopy, FTIR spectroscopy, XRD, FESEM, EDX, HRTEM, DLS, and zeta potential analyses...
July 31, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28755261/acinetobacter-pittii-an-emerging-new-multi-drug-resistant-fish-pathogen-isolated-from-diseased-blunt-snout-bream-megalobrama-amblycephala-yih-in-china
#6
Jie Li, Jinglong Cao, Xu Wang, Ning Liu, Weimin Wang, Yi Luo
Despite the reason that genus Acinetobacter works as a grave human pathogen, very few numbers of researches have been done so that term it as a pathogen in respect to fish. As per the current study, isolation of three pathogenic bacterial strains was carried out from infected blunt snout bream (Megalobrama amblycephala Yih), from a farm in Yixing city, Jiangsu province, China, which displayed symptoms like tail-rot, shedding scales and ascites in addition to gentle ulceration on the entire body regardless of size and sex of fish...
July 28, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28742284/aquatic-animals-promote-antibiotic-resistance-gene-dissemination-in-water-via-conjugation-role-of-different-regions-within-the-zebra-fish-intestinal-tract-and-impact-on-fish-intestinal-microbiota
#7
Jialun Fu, Dong Yang, Min Jin, Weili Liu, Xin Zhao, Chenyu Li, Tianyu Zhao, Jingfeng Wang, Zhixian Gao, Zhiqiang Shen, Zhigang Qiu, Jun-Wen Li
The aqueous environment is one of many reservoirs of antibiotic resistance genes (ARGs). Fish, as important aquatic animals which possess ideal intestinal niches for bacteria to grow and multiply, may ingest antibiotic resistance bacteria from aqueous environment. The fish gut would be a suitable environment for conjugal gene transfer including those encoding antibiotic resistance. However, little is known in relation to the impact of ingested ARGs or ARB on gut microbiota. Here, we applied the cultivation method, qPCR, nuclear molecular genetic marker and 16S rDNA amplicon sequencing technologies to develop a plasmid-mediated ARG transfer model of zebrafish...
July 25, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28715372/high-resolution-episcopic-microscopy-hrem-simple-and-robust-protocols-for-processing-and-visualizing-organic-materials
#8
Stefan H Geyer, Barbara Maurer-Gesek, Lukas F Reissig, Wolfgang J Weninger
We provide simple protocols for generating digital volume data with the high-resolution episcopic microscopy (HREM) method. HREM is capable of imaging organic materials with volumes up to 5 x 5 x 7 mm(3) in typical numeric resolutions between 1 x 1 x 1 and 5 x 5 x 5 µm(3). Specimens are embedded in methacrylate resin and sectioned on a microtome. After each section an image of the block surface is captured with a digital video camera that sits on the phototube connected to the compound microscope head. The optical axis passes through a green fluorescent protein (GFP) filter cube and is aligned with a position, at which the bock holder arm comes to rest after each section...
July 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28675217/g2-s16-dendrimer-as-a-candidate-for-a-microbicide-to-prevent-hiv-1-infection-in-women
#9
Rafael Ceña-Diez, Pilar García-Broncano, Francisco Javier de la Mata, Rafael Gómez, Salvador Resino, MªÁngeles Muñoz-Fernández
Unprotected heterosexual intercourse is the first route for sustaining the global spread of human immunodeficiency virus type 1 (HIV-1), being responsible for 80% of new HIV-1 infections in the world. The presence of inflammation in the female reproductive tract and the presence of semen increases the risk of heterosexual HIV-1 transmission. This state-of-the-art research based on an innovative nanotechnology design was focused on a toxicological study of the limitation of the activity of the novel H2O-soluble anionic carbosilane dendrimer G2-S16 in the adult cervical and foreskin epithelia...
July 13, 2017: Nanoscale
https://www.readbyqxmd.com/read/28672696/the-physiological-characteristics-of-zebra-fish-danio-rerio-based-on-metabolism-and-behavior-a-new-method-for-the-online-assessment-of-cadmium-stress
#10
Luhuizi Qi, Jingchun Ma, Jie Song, Shangge Li, Xiaoru Cui, Xiaojing Peng, Weiliang Wang, Zongming Ren, Mei Han, Ying Zhang
In order to illustrate heavy metal ecotoxicology associated with interactions between chemicals and biological systems, we investigated physiological changes (metabolism and behavior response) of zebra fish (Danio rerio) under 48 h Cadmium chloride (CdCl2) exposure using online monitoring technique. The concentrations of CdCl2 were designed as 4.26, 42.6 and 85.2 mg/L, which were 0.1, 1.0, and 2.0 based on toxic unit (TU), respectively. The metabolism was assessed using the oxygen consumption (OC), and the behavior response was analyzed in behavior strength (BS)...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28653157/computational-analysis-on-down-regulated-images-of-macrophage-scavenger-receptor
#11
Byeongtaek Oh, Yugyung Lee, Mingui Fu, Chi H Lee
BACKGROUND: Thiolated-graphene quantum dots (SH-GQDs) were developed and assessed for an efficient preventive means against atherosclerosis and potential toxicity through computational image analysis and animal model studies. EXPERIMENTS: Zebrafish (wild-type, wt) were used for evaluation of toxicity through the assessment of embryonic mortality, malformation and ROS generation. The amounts of SH-GQDs uptaken by mouse macrophage cells (Raw264.7) were analyzed using a flow cytometer...
June 26, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28616013/corrigendum-zebra-fish-lacking-adaptive-immunity-acquire-an-antiviral-alert-state-characterized-by-upregulated-gene-expression-of-apoptosis-multigene-families-and-interferon-related-genes
#12
Pablo García-Valtanen, Alicia Martínez-López, Azucena López-Muñoz, Melissa Bello-Perez, Regla M Medina-Gali, María Del Mar Ortega-Villaizán, Monica Varela, Antonio Figueras, Víctoriano Mulero, Beatriz Novoa, Amparo Estepa, Julio Coll
[This corrects the article on p. 121 in vol. 8, PMID: 28243233.].
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28608678/new-insights-into-the-stability-of-silver-sulfide-nanoparticles-in-surface-water-dissolution-through-hypochlorite-oxidation
#13
Lingxiangyu Li, Zhenlan Xu, Andreas Wimmer, Qinghua Tian, Xinping Wang
Silver sulfide nanoparticles (Ag2SNPs) are considered to be stable in the environment due to the extreme low solubility of Ag2S (Ksp: 6.3 × 10(-50)). Little is known about the stability of Ag2SNPs in surface water disinfected with aqueous chlorine, one of the globally most used disinfectants. Our results suggested that both uncoated and polyvinylpyrrolidone (PVP)-coated Ag2SNPs (100 μg/L) underwent dissolution in surface water disinfected with aqueous chlorine at a dose of 4 mg/L, showing the highest dissolved silver ion concentrations of 22...
June 27, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28558981/natural-lipids-based-nlc-containing-lidocaine-from-pre-formulation-to-in-vivo-studies
#14
Lígia N M Ribeiro, Márcia C Breitkreitz, Viviane A Guilherme, Gustavo H R da Silva, Verônica M Couto, Simone R Castro, Bárbara O de Paula, Daisy Machado, Eneida de Paula
In a nanotechnological approach we have investigated the use of natural lipids in the preparation of nanostructured lipid carriers (NLC). Three different NLC composed of copaiba oil and beeswax, sweet almond oil and shea butter, and sesame oil and cocoa butter as structural matrices were optimized using factorial analysis; Pluronic® 68 and lidocaine (LDC) were used as the colloidal stabilizer and model encapsulated drug, respectively. The optimal formulations were characterized by different techniques (IR-ATR, DSC, and TEM), and their safety and efficacy were also tested...
August 30, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28528958/investigation-of-calcium-dependent-activity-and-conformational-dynamics-of-zebra-fish-12-lipoxygenase
#15
Monica Mittal, Mahmudul Hasan, Navisraj Balagunaseelan, Alexander Fauland, Craig Wheelock, Olof Rådmark, Jesper Z Haeggström, Agnes Rinaldo-Matthis
BACKGROUND: A 12-lipoxygenase in zebra fish (zf12-LOX) was found to be required for normal embryonic development and LOXs are of great interest for targeted drug designing. In this study, we investigate the structural-functional aspects of zf12-LOX in response to calcium. METHODS: A soluble version of zf12-LOX was created by mutagenesis. Based on multiple sequence alignment, we mutated the putative calcium-responsive amino acids in N-PLAT domain of soluble zf12-LOX...
August 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28515779/higher-resource-level-promotes-virulence-in-an-environmentally-transmitted-bacterial-fish-pathogen
#16
Hanna Kinnula, Johanna Mappes, Janne K Valkonen, Katja Pulkkinen, Lotta-Riina Sundberg
Diseases have become a primary constraint to sustainable aquaculture, but remarkably little attention has been paid to a broad class of pathogens: the opportunists. Opportunists often persist in the environment outside the host, and their pathogenic features are influenced by changes in the environment. To test how environmental nutrient levels influence virulence, we used strains of Flavobacterium columnare, an environmentally transmitted fish pathogen, to infect rainbow trout and zebra fish in two different nutrient concentrations...
June 2017: Evolutionary Applications
https://www.readbyqxmd.com/read/28437728/is-a-a-o-process-effective-in-toxicity-removal-case-study-with-coking-wastewater
#17
Liu Shi, Dong Wang, Di Cao, Chunhong Na, Xie Quan, Ying Zhang
The anaerobic-anoxic-oxic (A/A/O) process is the commonly used biological wastewater treatment process, especially for the coking wastewater. However, limit is known about its ability in bio-toxicity removal from wastewater. In this study, we evaluated the performance of A/A/O process in bio-toxicity removal from the coking wastewater, using two test species (i.e. crustacean (Daphnia magna) and zebra fish (Danio rerio)) in respect of acute toxicity, oxidative damage and genotoxicity. Our results showed that the acute toxicity of raw influent was reduced gradually along with A/A/O process and the effluent presented no acute toxicity to Daphnia magna (D...
August 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28422363/the-occhiolino-occ-mutant-zebrafish-a-model-for-development-of-the-optical-function-in-the-biological-lens
#18
Masamoto Aose, Tor H Linbo, Owen Lawrence, Tadashi Senoo, David W Raible, John I Clark
BACKGROUND: Zebrafish visual function depends on quality optics. An F3 screen for developmental mutations in the Zebrafish nervous system was conducted in wild-type (wt) AB Zebrafish exposed to 3 mM of N-ethyl-N-nitrosourea (ENU). RESULTS: Mutant offspring, identified in an F3 screen, were characterized by a small pupil, resulting from retinal hypertrophy or hyperplasia and a small lens. Deficits in visual function made feeding difficult after hatching at approximately 5-6 days postfertilization (dpf)...
April 19, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28369860/broad-shifts-in-the-resource-use-of-a-commercially-harvested-fish-following-the-invasion-of-dreissenid-mussels
#19
Shannon A Fera, Michael D Rennie, Erin S Dunlop
Dreissenid mussels, including the zebra (Dreissena polymorpha) and quagga (Dreissena rostiformus bugensis) mussel, are invasive species known for their capacity to act as ecosystem engineers. They have caused significant changes in the many freshwater systems they have invaded by increasing water clarity, reducing primary productivity, and altering zooplankton and benthic invertebrate assemblages. What is less clear is how their ecosystem engineering effects manifest up the food web to impact higher trophic levels, including fish...
June 2017: Ecology
https://www.readbyqxmd.com/read/28344476/effect-of-pelargonidin-isolated-from-ficus-benghalensis-l-on-phenotypic-changes-in-zebrafish-danio-rerio-embryos
#20
Uday Kundap, Yogini Jaiswal, Rachana Sarawade, Leonard Williams, Mohd Farooq Shaikh
In the present study, the extraction and isolation of Pelargonidin, an anthocyanin compound from stem bark of Ficus benghalensis are described. The study also involves evaluation of the effect of Pelargonidin on phenotypic variations in zebra fish embryos. Extraction and isolation of Pelargonidin were carried out by employing liquid-liquid extraction technique, phytochemical tests, column chromatography, UV and FT-IR. In the zebra fish embryo model, Paclitaxel was employed as a negative control. A series of phenotypic changes in different stages of embryonic development were studied with treatment concentrations of Pelargonidin between 3...
February 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
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