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https://www.readbyqxmd.com/read/28192999/intracellular-and-in-vivo-cyanide-mapping-via-surface-plasmon-spectroscopy-of-single-au-ag-nanoboxes
#1
Peiyuan Wang, Yujie Bai, Chi Yao, Xiaomin Li, Lei Zhou, Wenxing Wang, Ahmed Mohamed El-Toni, Jian Zi, Dongyuan Zhao, Lei Shi, Fan Zhang
Cyanide is extremely toxic to organisms but difficult to detect in living biological specimens. Here, we report a new CN(-) sensing platform based on unmodified Au-Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN(-) concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference time-domain (FDTD) calculations are used to understand the CN(-) induced spectral shifts...
February 8, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28170238/dual-site-fluorescent-probe-for-visualizing-the-metabolism-of-cys-in-living-cells
#2
Yongkang Yue, Fangjun Huo, Peng Ning, Yongbin Zhang, Jianbin Chao, Xiangming Meng, Caixia Yin
Fluorescent probes as non-invasive tools to visualize the metabo-lism of bio-molecules hold great potential to explore their physio-logical and pathological processes. For cysteine (Cys), however, none of the reported fluorescent probes could image the metabolic processes in living cells. To achieve this goal, we developed a cou-marin derivate based on the rational design of the dual recognition sites for Cys and its metabolite SO2, respectively. The probe displayed distinct two channels turn-on fluorescent emission towards Cys and SO2, which were successfully applied for both A549 cells and zebra fish imaging...
February 7, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28156042/double-strand-dna-breaks-induced-by-paracyclophane-gold-i-complexes
#3
Peter Werner Roesky, Sebastian Bestgen, Carmen Seidl, Thomas Wiesner, Andreas Zimmer, Martina Falk, Beate Köberle, Martina Austeri, Jan Paradies, Stefan Bräse, Ute Schepers
Gold(I) complexes of ClickPhos [2.2]paracyclophane ligands were synthesized in excellent yields and fully characterized by spectroscopic methods as well as X-ray crystallography. The complexes exhibit a rigid ligand backbone and a triazolyl moiety and were systematically studied with respect to their cytotoxic properties. In combination with the ionic complex [(GemPhos)Au(tht)][ClO4] (tht = tetrahydrothiophene), in which the gold(I) atom exhibits a distorted trigonal coordination sphere of two phosphines and a labile tht ligand, their efficiency in cytotoxicity was investigated in HeLa, MCF7, and HCT116 cells as well as in a Zebra fish model...
February 3, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28129381/comparative-cytogenetics-between-two-important-songbird-models-the-zebra-finch-and-the-canary
#4
Michelly da Silva Dos Santos, Rafael Kretschmer, Carolina Frankl-Vilches, Antje Bakker, Manfred Gahr, Patricia C M O Brien, Malcolm A Ferguson-Smith, Edivaldo H C de Oliveira
Songbird species (order Passeriformes, suborder Oscines) are important models in various experimental fields spanning behavioural genomics to neurobiology. Although the genomes of some songbird species were sequenced recently, the chromosomal organization of these species is mostly unknown. Here we focused on the two most studied songbird species in neuroscience, the zebra finch (Taeniopygia guttata) and the canary (Serinus canaria). In order to clarify these issues and also to integrate chromosome data with their assembled genomes, we used classical and molecular cytogenetics in both zebra finch and canary to define their chromosomal homology, localization of heterochromatic blocks and distribution of rDNA clusters...
2017: PloS One
https://www.readbyqxmd.com/read/28091617/switch-from-sexual-to-parthenogenetic-reproduction-in-a-zebra-shark
#5
Christine L Dudgeon, Laura Coulton, Ren Bone, Jennifer R Ovenden, Severine Thomas
Parthenogenesis is a natural form of asexual reproduction in which embryos develop in the absence of fertilisation. Most commonly found in plants and invertebrate organisms, an increasing number of vertebrate species have recently been reported employing this reproductive strategy. Here we use DNA genotyping to report the first demonstration of an intra-individual switch from sexual to parthenogenetic reproduction in a shark species, the zebra shark Stegostoma fasciatum. A co-housed, sexually produced daughter zebra shark also commenced parthenogenetic reproduction at the onset of maturity without any prior mating...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28075531/three-dimensional-imaging-flow-cytometry-through-light-sheet-fluorescence-microscopy
#6
REVIEW
Emilio J Gualda, Hugo Pereira, Gabriel G Martins, Rui Gardner, Nuno Moreno
Flow cytometry is the tool of choice for high-speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two-dimensional spatial information were developed, some of the limitations remained though, namely constrains related to sample size and lack of depth or dynamic information. The combination of fluidics and light-sheet illumination has the potential to address these limitations...
February 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28043500/design-and-development-of-novel-inhibitors-for-the-treatment-of-latent-tuberculosis
#7
Rudraraju Srilakshmi Reshma, Perumal Yogeeswari, Dharmarajan Sriram
OBJECTIVE/BACKGROUND: "The captain of all these men of death", is the apt sobriquet for the age-old disease tuberculosis (TB). Despite the availability of many drugs, cases of increasing resistance in the forms of multi-drug and extensively drug-resistant TB and persistence [characteristic of Mycobacterium tuberculosis (MTB)] make the eradication of TB a nightmare. Approval of bedaquiline by the Food and Drug Administration focused attention on quinoline scaffolds for development of new anti-TB agents...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28002605/knockdown-of-the-putative-lifeguard-homologue-cg3814-in-neurons-of-drosophila-melanogaster
#8
P G M'Angale, B E Staveley
Lifeguard is an integral transmembrane protein that modulates FasL-mediated apoptosis by interfering with the activation of caspase 8. It is evolutionarily conserved, with homologues present in plants, nematodes, zebra fish, frog, chicken, mouse, monkey, and human. The Lifeguard homologue in Drosophila, CG3814, contains the Bax inhibitor-1 family motif of unknown function. Downregulation of Lifeguard disrupts cellular homeostasis and disease by sensitizing neurons to FasL-mediated apoptosis. We used bioinformatic analyses to identify CG3814, a putative homologue of Lifeguard, and knocked down CG3814/LFG expression under the control of the Dopa decarboxylase (Ddc-Gal4) transgene in Drosophila melanogaster neurons to investigate whether it possesses neuroprotective activity...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27999812/integrative-characterization-of-toxic-response-of-zebra-fish-danio-rerio-to-deltamethrin-based-on-ache-activity-and-behavior-strength
#9
Qing Ren, Tingting Zhang, Shangge Li, Zongming Ren, Meiyi Yang, Hongwei Pan, Shiguo Xu, Li Qi, Tae-Soo Chon
In order to characterize the toxic response of zebra fish (Danio rerio) to Deltamethrin (DM), behavior strength (BS) and muscle AChE activity of zebra fish were investigated. The results showed that the average values of both BS and AChE activity showed a similarly decreased tendency as DM concentration increased, which confirmed the dose-effect relationship, and high and low levels of AChE and BS partly matched low and high levels of exposure concentrations in self-organizing map. These indicated that AChE and BS had slight different aspects of toxicity although overall trend was similar...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27896025/zebra-mussel-beds-an-effective-feeding-ground-for-ponto-caspian-gobies-or-suitable-shelter-for-their-prey
#10
Jarosław Kobak, Małgorzata Poznańska, Łukasz Jermacz, Tomasz Kakareko, Daniel Prądzynski, Małgorzata Łodygowska, Karolina Montowska, Karolina Bącela-Spychalska
Aggregations of the Ponto-Caspian invasive zebra mussel (Dreissena polymorpha) constitute a suitable habitat for macroinvertebrates, considerably increasing their abundance and providing effective antipredator protection. Thus, the overall effect of a mussel bed on particular predator species may vary from positive to negative, depending on both prey density increase and predator ability to prey in a structurally complex habitat. Alien Ponto-Caspian goby fish are likely to be facilitated when introduced into new areas by zebra mussels, provided that they are capable of utilizing mussel beds as habitat and feeding grounds...
2016: PeerJ
https://www.readbyqxmd.com/read/27892606/svep1-plays-a-crucial-role-in-epidermal-differentiation
#11
L Samuelov, Q Li, R Bochner, N Najor, L Albrecht, N Malchin, T Goldsmith, M Grafi-Cohen, D Vodo, G Fainberg, B Meilik, I Goldberg, E Warshauer, T Rogers, S Edie, A Ishida-Yamamoto, L Burzenski, N Erez, S A Murray, A D Irvine, L Shultz, K Green, J Uitto, E Sprecher, O Sarig
SVEP1 is a recently identified multi-domain cell adhesion protein, homologous to the mouse polydom protein, which has been shown to mediate cell-cell adhesion in an integrin dependent-manner in osteogenic cells. In the present study, we characterized SVEP1 function in the epidermis. SVEP1 was found by qRT-PCR to be ubiquitously expressed in human tissues, including the skin. Confocal microscopy revealed that SVEP1 is normally mostly expressed in the cytoplasm of basal and suprabasal epidermal cells. Down-regulation of SVEP1 expression in primary keratinocytes resulted in decreased expression of major epidermal differentiation markers...
November 28, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27887759/implementing-an-open-access-casa-software-for-the-assessment-of-stallion-sperm-motility-relationship-with-other-sperm-quality-parameters
#12
Elisa Giaretta, Mauro Munerato, Marc Yeste, Giovanna Galeati, Marcella Spinaci, Carlo Tamanini, Gaetano Mari, Diego Bucci
Setting an open-access computer assisted sperm analysis (CASA) may benefit the evaluation of motility in mammalian sperm, especially when economic constraints do not allow the use of a commercial system. There have been successful attempts to develop such a device in Zebra fish sperm and the system has been used in very few studies on mammalian spermatozoa. Against this background, the present study aimed at developing an open-access CASA system for mammalian sperm using the horse as a model and based upon the Image J software previously established for Zebra fish sperm...
January 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/27858217/alternative-animal-and-non-animal-models-for-drug-discovery-and-development-bonus-or-burden
#13
Irlan Almeida Freires, Janaina de Cássia Orlandi Sardi, Ricardo Dias de Castro, Pedro Luiz Rosalen
Mammalian models have served as a basis for R&D over the past decades. Nevertheless, these models are expensive, laborious, may yield results that cannot always be translated into the human in vivo situation and, more recently, have reverberated great social and ethical dilemmas. Hence, the prospect of changes in the global scientific scenario and the Three Rs principle (Reduction, Replacement and Refinement) have encouraged the development of alternative methods to the use of mammals. Despite the efforts, suitable alternative tests are not available in all areas of biomedical research, as regulatory acceptance requires time, prior validation and robust financial and scientific investment...
November 17, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27825714/does-time-difference-of-the-acetylcholinesterase-ache-inhibition-in-different-tissues-exist-a-case-study-of-zebra-fish-danio-rerio-exposed-to-cadmium-chloride-and-deltamethrin
#14
Tingting Zhang, Meiyi Yang, Hongwei Pan, Shangge Li, Baigang Ren, Zongming Ren, Na Xing, Luhuizi Qi, Qing Ren, Shiguo Xu, Jie Song, Jingchun Ma
In order to illustrate time difference in toxic effects of cadmium chloride (CdCl2) and deltamethrin (DM), AChE activities were measured in different tissues, liver, muscle, brain, and gill, of Zebra fish (Danio rerio) across different concentrations in this research. The average AChE activity decreased comparing to 0.0 TU with DM (82.81% in 0.1 TU, 56.14% in 1.0 TU and 44.68% in 2.0 TU) and with CdCl2 (74.68% in 0.1 TU, 52.05% in 1.0 TU and 50.14% in 2.0 TU) showed an overall decrease with the increase of exposure concentrations...
February 2017: Chemosphere
https://www.readbyqxmd.com/read/27811302/retinal-specialization-through-spatially-varying-cell-densities-and-opsin-coexpression-in-cichlid-fish
#15
Brian E Dalton, Fanny de Busserolles, N Justin Marshall, Karen L Carleton
The distinct behaviours of animals and the varied habitats in which animals live place different requirements on their visual systems. A trade-off exists between resolution and sensitivity, with these properties varying across the retina. Spectral sensitivity, which affects both achromatic and chromatic (colour) vision, also varies across the retina, though the function of this inhomogeneity is less clear. We previously demonstrated spatially varying spectral sensitivity of double cones in the cichlid fish Metriaclima zebra owing to coexpression of different opsins...
January 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27630131/dynamic-sequence-evolution-of-a-sex-associated-b-chromosome-in-lake-malawi-cichlid-fish
#16
Frances E Clark, Matthew A Conte, Irani A Ferreira-Bravo, Andreia B Poletto, Cesar Martins, Thomas D Kocher
B chromosomes are extra chromosomes found in many species of plants, animals, and fungi. B chromosomes often manipulate common cellular processes to increase their frequency, sometimes to the detriment of organismal fitness. Here, we characterize B chromosomes in several species of Lake Malawi cichlid fish. Whole genome sequencing of Metriaclima zebra "Boadzulu" individuals revealed blocks of sequence with unusually high sequence coverage, indicative of increased copy number of those sequences. These regions of high sequence coverage were found only in females...
January 2017: Journal of Heredity
https://www.readbyqxmd.com/read/27601587/dhx33-transcriptionally-controls-genes-involved-in-the-cell-cycle
#17
Baolei Yuan, Xingshun Wang, Chunyan Fan, Jin You, Yuchu Liu, Jason D Weber, Hanbing Zhong, Yandong Zhang
The RNA helicase DHX33 has been shown to be a critical regulator of cell proliferation and growth. However, the underlying mechanisms behind DHX33 function remain incompletely understood. We present original evidence in multiple cell lines that DHX33 transcriptionally controls the expression of genes involved in the cell cycle, notably cyclin, E2F1, cell division cycle (CDC), and minichromosome maintenance (MCM) genes. DHX33 physically associates with the promoters of these genes and controls the loading of active RNA polymerase II onto these promoters...
December 1, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27600772/esxa-membrane-permeabilizing-activity-plays-a-key-role-in-mycobacterial-cytosolic-translocation-and-virulence-effects-of-single-residue-mutations-at-glutamine-5
#18
Qi Zhang, Decheng Wang, Guozhong Jiang, Wei Liu, Qing Deng, Xiujun Li, Wei Qian, Hugues Ouellet, Jianjun Sun
EsxA is required for virulence of Mycobacterium tuberculosis (Mtb) and plays an essential role in phagosome rupture and translocation to the cytosol of macrophages. Recent biochemical studies have demonstrated that EsxA is a membrane-permeabilizing protein. However, evidence that link EsxA membrane-permeabilizing activity to Mtb cytosolic translocation and virulence is lacking. Here we found that mutations at glutamine 5 (Q5) could up or down regulate EsxA membrane-permeabilizing activity. The mutation Q5K significantly diminished the membrane-permeabilizing activity, while Q5V enhanced the activity...
September 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27577911/study-on-glyco-modification-of-endostatin-derived-synthetic-peptide-endostatin2-es2-by-soluble-chitooligosaccharide
#19
Yang Yu, Feng Sun, Congcong Zhang, Zhendong Wang, Jinfeng Liu, Haining Tan
Soluble O-(2-hydroxyl)propyl-3-trimethyl ammonium chitooligosaccharide chloride (HTCOSC) was covalently conjugated to the 11-amino-acid peptide derived from amino terminus of endostatin (endostatin2, ES2, IVRRADRAAVP) to overcome its poor stability, low cell affinity and instable activity. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to study the structure and molecular weight information. The anti-angiogenic activities were evaluated using cell counting Kit-8 assay, flow cytometry assay, wounding migration assay, transwell migration assay, chicken chorioallantoic membrane (CAM) assay and zebra fish angiogenesis assay...
December 10, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27525509/6-gingerol-suppresses-adipocyte-derived-mediators-of-inflammation-in-vitro-and-in-high-fat-diet-induced-obese-zebra-fish
#20
Jia Choi, Kui-Jin Kim, Byung-Hak Kim, Eun-Jeong Koh, Min-Jung Seo, Boo-Yong Lee
The present study was performed to investigate the molecular mechanism of 6-gingerol on adipocyte-mediated systemic inflammation in vitro and in high-fat diet-induced obese zebra fish. 6-Gingerol decreased adipogenesis due to the suppression of adipocyte differentiation markers, including peroxisome proliferator-activated receptor gamma, CCAATT enhancer binding protein α, and adipocyte protein 2, and triglyceride synthesis enzymes, including sterol regulatory element-binding protein-1, fatty acid synthase, lysophosphatidic acid acyltransferase, and acyl-coA : diacylglycerol acyltransferase 1, in 3T3-L1...
February 2017: Planta Medica
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