keyword
MENU ▼
Read by QxMD icon Read
search

Zebra fish

keyword
https://www.readbyqxmd.com/read/29152025/advances-in-microfluidic-devices-made-from-thermoplastics-used-in-cell-biology-and-analyses
#1
REVIEW
Elif Gencturk, Senol Mutlu, Kutlu O Ulgen
Silicon and glass were the main fabrication materials of microfluidic devices, however, plastics are on the rise in the past few years. Thermoplastic materials have recently been used to fabricate microfluidic platforms to perform experiments on cellular studies or environmental monitoring, with low cost disposable devices. This review describes the present state of the development and applications of microfluidic systems used in cell biology and analyses since the year 2000. Cultivation, separation/isolation, detection and analysis, and reaction studies are extensively discussed, considering only microorganisms (bacteria, yeast, fungi, zebra fish, etc...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29127928/native-hypersaline-sulphate-reducing-bacteria-contributes-to-iron-nanoparticle-formation-in-saltpan-sediment-a-concern-for-aquaculture
#2
Kirti Ranjan Das, Meenal Kowshik, M K Praveen Kumar, Savita Kerkar, S K Shyama, Samir Mishra
A hypersaline dissimilatory sulphate reducing bacterium, strain LS4, isolated from the sediments of Ribander saltpan, Goa, India was found to produce (Fe2O3) maghemite nanoparticles. The presence of maghemite nanoparticles was also detected in the same sediment. Strain LS4 was isolated anaerobically on modified Hatchikian's media at 300 psu, growing optimally at 30 °C, 150 psu salinity and pH 7.8. Based on biochemical characteristics and 16S rRNA sequence analysis, the strain LS4 belongs to genus Desulfovibrio...
November 8, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29126392/mir-17-5p-suppresses-cell-proliferation-and-invasion-by-targeting-etv1-in-triple-negative-breast-cancer
#3
Jie Li, Yuanhui Lai, Jieyi Ma, Yue Liu, Jiong Bi, Longjuan Zhang, Lianzhou Chen, Chen Yao, Weiming Lv, Guangqi Chang, Shenming Wang, Mao Ouyang, Wenjian Wang
BACKGROUND: Triple-negative breast cancer (TNBC) is the malignancy with the worst outcome among all breast cancer subtypes. We reported that ETV1 is a significant oncogene in TNBC tumourigenesis. Consequently, investigating the critical regulatory microRNAs (miRNAs) of ETV1 may be beneficial for TNBC targeted therapy. METHODS: We performed in situ hybridization (ISH) and immunohistochemistry (IHC) to detect the location of miR-17-5p and ETV1 in TNBC patient samples, respectively...
November 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29122587/the-splicing-code
#4
REVIEW
Marco Baralle, Francisco Ernesto Baralle
This issue dedicated to the code of life tackles very challenging and open questions in Biology. The genetic code, brilliantly uncovered over 50 years ago is an example of a univocal biological code. In fact, except for very few and marginal variations, it is the same from bacteria to man, the RNA stretch: 5' GUGUUC 3' reads as the dipeptide: Val-Phe in bacteria, in yeast, in Arabidopsis, in zebra fish, in mouse and in human. A degree of ambiguity is possible if mutations are introduced in the tRNAs in a way that the anticodon reads one amino acid but the aminoacyl-transferase attaches a different one onto the tRNA...
November 6, 2017: Bio Systems
https://www.readbyqxmd.com/read/29044046/locally-extensive-meningoencephalitis-caused-by-miamiensis-avidus-syn-philasterides-dicentrarchi-in-a-zebra-shark
#5
Wen-Ta Li, Chieh Lo, Chen-Yi Su, Hsuan Kuo, Susanne Je-Han Lin, Hui-Wei Chang, Victor Fei Pang, Chian-Ren Jeng
Scuticociliatosis, caused by ciliated protozoa in the subclass Scuticociliatia of the phylum Ciliophora, can cause fatal disease in teleost fish species. However, information on scuticociliatosis in elasmobranchs is still scarce. In this report, we describe a case of locally extensive meningoencephalitis caused by Miamiensis avidus (syn. Philasterides dicentrarchi) in a 2 yr old captive zebra shark Stegostoma fasciatum. Granulocytic meningoencephalitis was observed through histological assessment. Inflammation was confined to the ventral aspect of the brain with a large number of ciliated protozoa, transforming into non-suppurative meningitis in the lateral aspect, and gradually vanished in the dorsal aspect...
October 18, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29028984/a-bayesian-framework-for-the-inference-of-gene-regulatory-networks-from-time-and-pseudo-time-series-data
#6
M Sanchez-Castillo, D Blanco, I M Tienda-Luna, M C Carrion, Yufei Huang
Motivation: Molecular profiling techniques have evolved to single-cell assays, where dense molecular profiles are screened simultaneously for each cell in a population. High-throughput single-cell experiments from a heterogeneous population of cells can be experimentally and computationally sorted as a sequence of samples pseudo-temporally ordered samples. The analysis of these data sets, comprising a large number of samples, has the potential to uncover the dynamics of the underlying regulatory programmes...
September 25, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28930614/inter-species-functional-interactome-of-nuclear-steroid-receptors-r1
#7
Styliani A Geronikolou, Athanasia Pavlopoulou, Christina Kanaka-Gantenbein, George Chrousos
Steroids exert their actions by binding to the glucocorticoid, mineralocorticoid, androgen, estrogen and progesterone classes of receptors. Despite an exponential increase in our knowledge of steroid receptors, their interactions with other molecules, subcellular location and functions still need further elucidation. To unravel the mechanism(s) of action of the steroid hormones, as well as the function of their cognate nuclear receptors, an interaction network was created (henceforth referred to as "R1 Interactome")- illustrating that robust interactions have been preserved in rodents, frog, zebra fish and drosophila...
January 1, 2018: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/28913548/changes-in-histopathology-enzyme-activities-and-the-expression-of-relevant-genes-in-zebrafish-danio-rerio-following-long-term-exposure-to-environmental-levels-of-thallium
#8
Li-Ping Hou, Yang Yang, Hu Shu, Guang-Guo Ying, Jian-Liang Zhao, Yi-Bing Chen, Yong-Heng Chen, Gui-Zhen Fang, Xin Li, Ji-Sheng Liu
Thallium is a rare-earth element, but widely distributed in water environments, posing a potential risk to our health. This study was designed to investigate the chronic effects of thallium based on physiological responses, gene expression, and changes in the activity of relevant enzymes in adult zebra fish exposed to thallium at low doses. The endpoints assessed include mRNA expression of metallothionein (MT)2 and heat shock protein HSP70; enzymatic activities of superoxide dismutase (SOD) and Na(+)/K(+)-ATPase; and the histopathology of gill, gonad, and liver tissues...
November 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28876356/a-versatile-upconversion-surface-evaluation-platform-for-bio-nano-surface-selection-for-the-nervous-system
#9
Libing Fu, Marco Morsch, Bingyang Shi, Guoying Wang, Albert Lee, Rowan Radford, Yiqing Lu, Dayong Jin, Roger Chung
There is considerable interest in developing diagnostic nanotools for early detection and delivery of various therapeutic agents for treatment of neurodegenerative diseases. However, a key challenge remains in the selection of suitable surfaces to overcome the nano-bio interface issue, namely that many nanoparticle surfaces demonstrate instability when administered into biological environments and show substantial cytotoxicity to the central nervous system. In this study, we fabricated an evaluation platform for bio-nano surface selection based on the combination of upconversion nanoparticles (UCNPs), cultured neural cells and zebra fish, and systemically demonstrated how it can evaluate the suitability of nanoparticle surfaces for applications in the central nervous system...
September 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28869925/cadmium-stress-assessment-based-on-the-electrocardiogram-characteristics-of-zebra-fish-danio-rerio-qrs-complex-could-play-an-important-role
#10
Na Xing, Lizhen Ji, Jie Song, Jingchun Ma, Shangge Li, Zongming Ren, Fei Xu, Jianping Zhu
The electrocardiogram (ECG) of zebra fish (Danio rerio) expresses cardiac features that are similar to humans. Here we use sharp microelectrode measurements to obtain ECG characteristics in adult zebra fish and analyze the effects of cadmium chloride (CdCl2) on the heart. We observe the overall changes of ECG parameters in different treatments (0.1 TU, 0.5 TU and 1.0 TU CdCl2), including P wave, Q wave, R wave, S wave, T wave, PR interval (atrial contraction), QRS complex (ventricular depolarization), ST segment, and QT interval (ventricular repolarization)...
August 31, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28862536/investigation-of-a-brazilian-tannery-effluent-by-means-of-zebra-fish-danio-rerio-embryo-acute-toxicity-fet-test
#11
Otávio Pelegrino Rocha, Danielle Palma De Oliveira
Tannery effluents consist of a complex chemical composition not only limited to primary pollutants, which also require biological detection as these compounds may produce adverse effects. The fish embryo toxicity (FET) test with Danio rerio is an alternative method in hazard and risk assessment for determination of chemical-mediated effects. The aim of this investigation was to use the FET test to detect compounds and consequent effects in Brazilian tannery effluents. Samples were collected from the inlet and outlet of the effluent treatment plant at a tannery located in Restinga, São Paulo, Brazil...
September 1, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28855876/the-non-peptide-arginine-vasopressin-v1a-selective-receptor-antagonist-sr49059-blocks-the-rewarding-prosocial-and-anxiolytic-effects-of-3-4-methylenedioxymethamphetamine-and-its-derivatives-in-zebra-fish
#12
Luisa Ponzoni, Daniela Braida, Gianpietro Bondiolotti, Mariaelvina Sala
3,4-Methylenedioxymethamphetamine (MDMA) and its derivatives, 2,5-dimethoxy-4-bromo-amphetamine hydrobromide (DOB) and para-methoxyamphetamine (PMA), are recreational drugs whose pharmacological effects have recently been attributed to serotonin 5HT2A/C receptors. However, there is growing evidence that the oxytocin (OT)/vasopressin system can modulate some the effects of MDMA. In this study, MDMA (2.5-10 mg/kg), DOB (0.5 mg/kg), or PMA (0.005, 0.1, or 0.25 mg/kg) were administered intramuscularly to adult zebra fish, alone or in combination with the V1a vasopressin antagonist, SR49059 (0...
2017: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/28806601/influence-of-sublethal-doses-of-acetamiprid-and-halosulfuron-methyl-on-metabolites-of-zebra-fish-brachydanio-rerio
#13
Hong Zhang, Lijuan Zhao
Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to investigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio)...
August 4, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28775072/lmo2-lim-domain-only-2-modulates-sphk1-sphingosine-kinase-and-promotes-endothelial-cell-migration
#14
Gianfranco Matrone, Shu Meng, Qilin Gu, Jie Lv, Longhou Fang, Kaifu Chen, John P Cooke
OBJECTIVE: Lmo (LIM-domain-only)2 transcription factor is involved in hematopoiesis and vascular remodeling. Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). We hypothesized that Lmo2 regulates Sphk1 to promote endothelial cell (EC) migration and vascular development. APPROACH AND RESULTS: Lmo2 and Sphk1 knockdown (KD) were performed in Tg(fli1:EGFP) (y1) zebrafish and in human umbilical vein EC. Rescue of phenotypes or overexpression of these factors were achieved using mRNA encoding Lmo2 or Sphk1...
October 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28774750/advances-in-nonhuman-primate-models-of-autism-integrating-neuroscience-and-behavior
#15
REVIEW
M D Bauman, C S Schumann
Given the prevalence and societal impact of autism spectrum disorders (ASD), there is an urgent need to develop innovative preventative strategies and treatments to reduce the alarming number of cases and improve core symptoms for afflicted individuals. Translational efforts between clinical and preclinical research are needed to (i) identify and evaluate putative causes of ASD, (ii) determine the underlying neurobiological mechanisms, (iii) develop and test novel therapeutic approaches and (iv) ultimately translate basic research into safe and effective clinical practices...
August 1, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28766927/combining-hyperspectral-imaging-and-chemometrics-to-assess-and-interpret-the-effects-of-environmental-stressors-on-the-organism-at-tissue-level
#16
Víctor Olmos, Mònica Marro, Pablo Loza-Alvarez, Demetrio Raldúa, Eva Prats, Francesc Padrós, Benjamin Piña, Romà Tauler, Anna de Juan
Changes on an organism by the exposure to environmental stressors may be characterized by hyperspectral images (HSI), which preserve the morphology of biological samples, and suitable chemometric tools. The approach proposed allows assessing and interpreting the effect of contaminant exposure on heterogeneous biological samples monitored by HSI at specific tissue levels. In this work, the model example used consists of the study of the effect of the exposure of chlorpyrifos-oxon on zebra fish tissues. To assess this effect, unmixing of the biological sample images followed by tissue-specific classification models based on the unmixed spectral signatures is proposed...
August 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28762049/biogenic-synthesis-of-gold-nanoparticles-from-terminalia-arjuna-bark-extract-assessment-of-safety-aspects-and-neuroprotective-potential-via-antioxidant-anticholinesterase-and-antiamyloidogenic-effects
#17
Natarajan Suganthy, Vijayan Sri Ramkumar, Arivalagan Pugazhendhi, Giovanni Benelli, Govindaraju Archunan
The development of neuroprotective drugs through eco-friendly production routes is a major challenge for current pharmacology. The present study was carried out to synthesize gold nanoparticles (AuNPs) through biogenic route using ethanolic bark extract of Terminalia arjuna, a plant of high interest in Asian traditional medicine, and to evaluate its neuroprotective effects. The synthesized AuNPs were characterized by UV-Vis spectroscopy, FTIR spectroscopy, XRD, FESEM, EDX, HRTEM, DLS, and zeta potential analyses...
July 31, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28755261/acinetobacter-pittii-an-emerging-new-multi-drug-resistant-fish-pathogen-isolated-from-diseased-blunt-snout-bream-megalobrama-amblycephala-yih-in-china
#18
Jie Li, Jinglong Cao, Xu Wang, Ning Liu, Weimin Wang, Yi Luo
Despite the reason that genus Acinetobacter works as a grave human pathogen, very few numbers of researches have been done so that term it as a pathogen in respect to fish. As per the current study, isolation of three pathogenic bacterial strains was carried out from infected blunt snout bream (Megalobrama amblycephala Yih), from a farm in Yixing city, Jiangsu province, China, which displayed symptoms like tail-rot, shedding scales and ascites in addition to gentle ulceration on the entire body regardless of size and sex of fish...
July 28, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28742284/aquatic-animals-promote-antibiotic-resistance-gene-dissemination-in-water-via-conjugation-role-of-different-regions-within-the-zebra-fish-intestinal-tract-and-impact-on-fish-intestinal-microbiota
#19
Jialun Fu, Dong Yang, Min Jin, Weili Liu, Xin Zhao, Chenyu Li, Tianyu Zhao, Jingfeng Wang, Zhixian Gao, Zhiqiang Shen, Zhigang Qiu, Jun-Wen Li
The aqueous environment is one of many reservoirs of antibiotic resistance genes (ARGs). Fish, as important aquatic animals which possess ideal intestinal niches for bacteria to grow and multiply, may ingest antibiotic resistance bacteria from aqueous environment. The fish gut would be a suitable environment for conjugal gene transfer including those encoding antibiotic resistance. However, little is known in relation to the impact of ingested ARGs or antibiotic resistance bacteria (ARB) on gut microbiota. Here, we applied the cultivation method, qPCR, nuclear molecular genetic marker and 16S rDNA amplicon sequencing technologies to develop a plasmid-mediated ARG transfer model of zebrafish...
July 25, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28715372/high-resolution-episcopic-microscopy-hrem-simple-and-robust-protocols-for-processing-and-visualizing-organic-materials
#20
Stefan H Geyer, Barbara Maurer-Gesek, Lukas F Reissig, Wolfgang J Weninger
We provide simple protocols for generating digital volume data with the high-resolution episcopic microscopy (HREM) method. HREM is capable of imaging organic materials with volumes up to 5 x 5 x 7 mm(3) in typical numeric resolutions between 1 x 1 x 1 and 5 x 5 x 5 µm(3). Specimens are embedded in methacrylate resin and sectioned on a microtome. After each section an image of the block surface is captured with a digital video camera that sits on the phototube connected to the compound microscope head. The optical axis passes through a green fluorescent protein (GFP) filter cube and is aligned with a position, at which the bock holder arm comes to rest after each section...
July 7, 2017: Journal of Visualized Experiments: JoVE
keyword
keyword
80318
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"