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Raw 264.7

Ming-Yue Wang, Chang Shen, Meng-Fei An, Chuan-Qi Xie, Xin Wu, Qiang-Qiang Zhu, Bin Sun, Yan-Ping Huang, Yun-Li Zhao, Xuan-Jun Wang, Jun Sheng
AIMS: Dendrobium candidum (DC) and black tea, are traditional chinese drinks, which contain multiple active ingredients. However, whether or not the combination of these two ingredients can improve osteoporosis remains unknown. This study therefore aimed to examine the effects of the combination of DC and black tea extract (BTE) on osteoporosis. MAIN METHODS: Ovariectomy (OVX)-induced osteoporosis in vivo as well as receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis in vitro was selected...
March 12, 2018: Life Sciences
Vahid Mirshafiee, Bingbing Sun, Chong Hyun Chang, Yu-Pei Liao, Wen Jiang, Jinhong Jiang, Xiangsheng Liu, Xiang Wang, Tian Xia, Andre E Nel
The liver and the mononuclear phagocyte system (MPS) is a frequent target for engineered nanomaterials (ENMs), either as a result of particle uptake and spread from primary exposure sites or systemic administration of therapeutic and imaging nanoparticles. In this study, we performed a comparative analysis of the toxicological impact of 29 metal oxide (MOx) nanoparticles (NPs), some commonly used in consumer products, in transformed or primary Kupffer cells (KCs) and hepatocytes. Not only did we observe differences between KC and hepatocytes, but also differences in the toxicological profiles of transition metal oxides (TMOs, e...
March 15, 2018: ACS Nano
Bo Yang, Nathan Heath Patterson, Tina Tsui, Richard M Caprioli, Jeremy L Norris
It has been widely recognized that individual cells that exist within a large population of cells, even if they are genetically identical, can have divergent molecular makeups resulting from a variety of factors, including local environmental factors and stochastic processes within each cell. Presently, numerous approaches have been described that permit the resolution of these single-cell expression differences for RNA and protein; however, relatively few techniques exist for the study of lipids and metabolites in this manner...
March 13, 2018: Journal of the American Society for Mass Spectrometry
Kangfeng Jiang, Shuai Guo, Tao Zhang, Yaping Yang, Gan Zhao, Aftab Shaukat, Haichong Wu, Ganzhen Deng
Acute lung injury (ALI) is a progressive clinical disease with a high mortality rate, and characterized by an excessive uncontrolled inflammatory response. MicroRNAs (miRNAs) play a critical role in various human inflammatory diseases, and have been recognized as important regulators of inflammation. However, the regulatory mechanisms mediated by miRNAs involved in Lipopolysaccharide (LPS)-induced inflammation in ALI remain hazy. In this study, we found that miR-181a expression in the lung tissues of ALI mice and LPS-stimulated RAW 264...
2018: Frontiers in Pharmacology
Shaoyi Cai, Gengzheng Zhu, Xiaohong Cen, Jingjie Bi, Jingru Zhang, Xiaoshan Tang, Kun Chen, Kui Cheng
Toll-like receptor 2 (TLR2) can recognize pathogen-associated molecular patterns to defense against invading organisms and has been represents an attractive therapeutic target. Until today, none TLR2 small molecule antagonist have been developed in clinical trial. Herein, we designed and synthesized 50 N-benzylideneaniline compounds with the help of CADD. And subsequent in vitro studies leading to the optimized compound SMU-A0B13 with most potent inhibitory activity to TLR2 (IC50 =18.21 ± 0.87 μM)...
March 10, 2018: Bioorganic & Medicinal Chemistry
Da Yeon Lee, Hwa Jin Lee, Jae-Ha Ryu
Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols ( 1 - 7 ) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols ( 2 - 4 and 6 ) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2 - 4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS)...
March 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Li-Hao Huang, Elaina M Melton, Haibo Li, Paul Sohn, DaeYoung Jung, Ching-Yi Tsai, Tian Ma, Hiroyuki Sano, HyeKyung Ha, Randall H Friedline, Jason K Kim, Edward Usherwood, Catherine Cy Chang, Ta-Yuan Chang
Macrophages are phagocytes that play important roles in health and diseases. Acyl-CoA:cholesterol acyltransferase 1 [ACAT1] converts cellular cholesterol to cholesteryl esters, and is expressed in many cell types. Unlike global Acat1 knockout [KO], myeloid-specific Acat1 KO [Acat1-M/-M ] does not cause overt abnormalities in mice. Here we performed analyses in age and sex matched Acat1-M/-M and wild-type mice on chow or Western diet, and discovered that Acat1-M/-M mice exhibit resistance to Western diet-induced obesity...
March 13, 2018: American Journal of Physiology. Endocrinology and Metabolism
Francine Kengne Mediesse, Thaddée Boudjeko, Anantharaju Hasitha, Matharasala Gangadhar, Wilfred Fon Mbacham, Perumal Yogeeswari
BACKGROUND: Khaya grandifoliola (C.D.C.) stem bark, Cymbopogon citratus (Stapf) and Cryptolepis sanguinolenta (Lindl.) Schltr leaves are used in Cameroonian traditional medicine for the treatment of inflammatory diseases. Several studies have been performed on the biological activities of secondary metabolites extracted from these plants. However, to the best of our knowledge, the anti-neuro inflammatory and protective roles of the polysaccharides of these three plants have not yet been elucidated...
March 12, 2018: BMC Complementary and Alternative Medicine
Ivanna S Rolny, Silvia M Racedo, Pablo F Pérez
In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502...
December 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
Yannick P Borguet, Sarosh Khan, Amandine Noel, Sean P Gunsten, Steven L Brody, Mahmoud Elsabahy, Karen L Wooley
To expand the range of functional polymer materials to include fully hydrolytically degradable systems that bear bioinspired phosphorus-containing linkages both along the backbone and as cationic side chain moieties for packaging and delivery of nucleic acids, phosphonium-functionalized polyphosphoester- block-poly(l-lactide) copolymers of various compositions were synthesized, fully characterized, and their self-assembly into nanoparticles were studied. First, an alkyne-functionalized polyphosphoester- block-poly(l-lactide) copolymer was synthesized via a one pot sequential ring opening polymerization of an alkyne-functionalized phospholane monomer, followed by the addition of l-lactide to grow the second block...
March 11, 2018: Biomacromolecules
Xiao Wang, Xiumin Wang, Da Teng, Ruoyu Mao, Ya Hao, Na Yang, Zhanzhan Li, Jianhua Wang
Treatment of Staphylococcus aureus infections remains very difficult due to its capacity to survive intracellularly and its multidrug resistance. In this study, the extracellular/intracellular activities of plectasin derivatives-MP1102/NZ2114 were investigated against three methicillin-susceptible/-resistant S. aureus (MSSA/MRSA) strains in RAW 264.7 macrophages and mice to overcome poor intracellular activity. Antibacterial activities decreased 4-16-fold under a mimic phagolysosomal environment. MP1102/NZ2114 were internalized into the cells via clathrin-mediated endocytosis and macropinocytosis and distributed in the cytoplasm; they regulated tumor necrosis factor-α, interleukin-1β and interleukin-10 levels...
March 9, 2018: Scientific Reports
Katarzyna Miranowicz-Dzierżawska
The aim of this study was to assess whether there are differences between the results of determining oxidative stress markers obtained from different origin cell lines after exposure to chemicals generating free radicals. The studies considered two markers of oxidative stress: the level of thiobarbituric acid reactive substances (TBARS) and superoxide dismutase activity. The evaluation was performed in five cell lines: Chinese hamster ovary (CHO-9) cells, lung adenocarcinoma A549, macrophages RAW264.7, skin carcinoma cells A431, and keratinocytes HaCaT...
January 1, 2018: Toxicology and Industrial Health
Tzenge-Lien Shih, Ming-Hwa Liu, Chia-Wai Li, Chia-Feng Kuo
A series of B-ring, halo-substituted chalcones and azachalcones were synthesized to evaluate and compare their anti-inflammatory activity. Mouse BALB/c macrophage RAW 264.7 were pre-treated with 10 μg/mL of each compound for one hour before induction of inflammation by lipopolysaccharide (1 μg/mL) for 6 h. Some halo-chalcones and -azachalcones suppressed expression of pro-inflammatory factors toll-like receptor 4 (TLR4), IκB-α, transcription factor p65, interleukine 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), and cyclooxygenase 2 (COX-2)...
March 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Chen Zhang, Ran Wen, Xiao-Li Ma, Ke-Wu Zeng, Yang Xue, Pu-Ming Zhang, Ming-Bo Zhao, Yong Jiang, Guo-Qing Liu, Peng-Fei Tu
Two new disesquiterpenoids (1 and 2) and 11 new (3-13) and 10 known (14-23) sesquiterpenoids were isolated from the whole plants of Artemisia freyniana. Their structures were elucidated by spectroscopic data analysis and comparison with published NMR data. The absolute configurations of the new isolates (1-13) were assigned based on single-crystal X-ray diffraction data and comparison of the experimental and calculated ECD data. The eremophilane derivatives 8 and 9 possess an unprecedented 2-isopropyl-3,7,7a-trimethyl-2,4,5,6,7,7a-hexahydro-1 H-indene scaffold, and a putative biosynthetic pathway for these compounds is proposed...
March 8, 2018: Journal of Natural Products
Mithun Ghosh, In Sook Kim, Young Min Lee, Seong Min Hong, Taek Hwan Lee, Ji Hong Lim, Trishna Debnath, Beong Ou Lim
This study aimed to determine the anti-osteoclastogenic effects of extracts from Aronia melanocarpa 'Viking' (AM) and identify the underlying mechanisms in vitro. Reactive oxygen species (ROS) are signal mediators in osteoclast differentiation. AM extracts inhibited ROS production in RAW 264.7 cells in a dose-dependent manner and exhibited strong radical scavenging activity. The extracts also attenuated the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts. To attain molecular insights, the effect of the extracts on the signaling pathways induced by receptor activator of nuclear factor kappa B ligand (RANKL) were also investigated...
March 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Tian-Yun Jin, Shao-Qiang Li, Cui-Rong Jin, Hao Shan, Rui-Min Wang, Ming-Xing Zhou, Ai-Ling Li, Ling-Yu Li, Shui-Yao Hu, Tao Shen, Lan Xiang
Isoquinoline alkaloids possess a wide range of structural features and pharmaceutical activities and are promising drug candidates. Ten water-soluble catecholic isoquinolines were isolated from the medicinal plant Portulaca oleracea, including three new (1-3) and seven known compounds (4-10), along with the known catecholamines 11 and 12 and four other known compounds (13-16). A method of polyamide column chromatography using EtOAc-MeOH as the mobile phase was developed for the isolation of catecholic isoquinolines...
March 8, 2018: Journal of Natural Products
Chris Steffi, Dong Wang, Chee Hoe Kong, Zuyong Wang, Poon Nian Lim, Zhilong Shi, Eng San Thian, Wilson Wang
Estrogen, a steroid hormone, plays an important role in modulating osteoclast proliferation and development. Estrogen deficiency boosts osteoclast activity, leading to osteoporosis in elderly women. In this study, 17-ß estradiol (E2) loaded poly(ε-caprolactone) (PCL)/silk fibroin (SF) electrospun microfibers was developed as a proposed localized E2 delivery system to treat osteoporotic fractures. PCL is a synthetic polymer known for its biocompatibility and excellent mechanical properties. The bioactivity of PCL was enhanced by mixing it with natural silk fibroin (SF) polymer that has low immunogenicity and inherent bioactivity...
March 7, 2018: ACS Applied Materials & Interfaces
Rongjun Liu, Liangliang Zhang, Yunyun Chen, Zirong Huang, Yong Huang, Shulin Zhao
Superoxide anion (O2•-) and hydroxyl radical (•OH) are important reactive oxygen species (ROS) used as biomarkers in physiological and pathological processes. The ROS generation is closely related to the development of a variety of inflammatory diseases. However, in situ tracing inflammation process by real time monitoring the changes of ROS is difficult owing to short half-lives of ROS and high tissue autofluorescence in vivo. Here we developed a new near-infrared (NIR) ratiometric fluorescence imaging approach by using a förster resonance energy transfer (FRET)-base ratiometric fluorescent nanoprobe for real-time monitoring O2•- and •OH generation, and in situ tracing inflammation process in vivo...
March 7, 2018: Analytical Chemistry
Agnese Gugliandolo, Sabrina Giacoppo, Marcello Ficicchia, Angelo Aliquò, Placido Bramanti, Emanuela Mazzon
Certain nutrients are able to exert health promoting effects. The consumption of Brassicaceae vegetables has increased given their reported beneficial effects on human health, due to their high content of nutraceutical compounds. The health benefits appear to be associated with the presence of glucosinolates and flavonoids. Certain nutraceutics have been revealed to have anti‑inflammatory action. In the present study, the anti‑inflammatory properties of Eruca sativa seed extract (ESE) were evaluated in NSC‑34 motor neurons exposed to the cell culture medium of lipopolysaccharide (LPS)‑stimulated RAW 264...
March 7, 2018: Molecular Medicine Reports
Nathalia Regina F Leal, Mariana Vieira Vigliano, Fabiana A Pinto, Thayane V de Sousa, Leosvaldo S M Velozo, Kátia C C Sabino, Maria da Graça Justo, Marsen Garcia Pinto Coelho
OBJECTIVES: To evaluate the anti-inflammatory potential of Pterodon polygalaeflorus hexane extract (HE) and its fractions on macrophage migration in vitro and in vivo. METHODS: Hexane extract from P. polygalaeflorus fruits was fractionated and yielded four fractions. RAW 264.7 cells were treated with samples to evaluate cell viability (MTT assay), cell migration (wound healing and transwell assays), CD14 expression (flow cytometry), iNOS and cytokine mRNA expression (RT-qPCR), NO (Griess reaction) and cytokine (ELISA) production...
March 7, 2018: Journal of Pharmacy and Pharmacology
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