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https://www.readbyqxmd.com/read/28738536/effect-of-apigenin-kaempferol-and-resveratrol-on-the-gene-expression-and-protein-secretion-of-tumor-necrosis-factor-alpha-tnf-%C3%AE-and-interleukin-10-il-10-in-raw-264-7-macrophages
#1
Marta Palacz-Wrobel, Paulina Borkowska, Monika Paul-Samojedny, Malgorzata Kowalczyk, Anna Fila-Danilow, Renata Suchanek-Raif, Jan Kowalski
Polyphenols such as apigenin, kaempferol or resveratrol are typically found in plants, including fruits, vegetables, herbs and spices, which have a wide range of biological functions such as antioxidative, anti-inflammatory, vasodilative, anticoagulative and proapoptotic. Discovering such multifunctional compounds in widely consumed plant-based products - ones that both inhibit the release of TNF-α from tissue macrophages and at the same time enhance the secretion of IL-10 - would be an important signpost in the quest for effective pharmacological treatment of numerous diseases that have an inflammatory etiology...
July 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28732811/kaurenoic-acid-activates-tgf-%C3%AE-signaling
#2
Kyun Ha Kim, Jin Woo Han, Sung-Ki Jung, Bum-Joon Park, Chang Woo Han, Myungsoo Joo
BACKGROUND: Kaurenoic acid (ent-kaur-16-en-19-oic acid: KA) is a key constituent found in the roots of Aralia continentalis Kitagawa (Araliaceae) that has been used for treating rheumatism in traditional Asian medicine. HYPOTHESIS: Although KA was reported to suppress inflammation by activating Nrf2, the anti-inflammatory function of KA is less characterized. Given the complex nature of the inflammatory response and the critical role of TGF-β in resolving inflammation, we hypothesized that KA suppresses inflammatory response by activating TGF-β signaling...
August 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28726500/chemical-constituents-from-curcuma-longa-l-and-their-inhibitory-effects-of-nitric-oxide-production
#3
Ting Yuan, Cuiyun Zhang, Chongyue Qiu, Guiyang Xia, Fei Wang, Bin Lin, Hua Li, Lixia Chen
A new bisabolane-type sesquiterpenoid, turmerone Q (1), along with six known compounds (2-7), were isolated from the rhizomes of Curcuma longa L. The structural elucidation of the new compound was conducted using (1)H NMR, (13)C NMR, HSQC, HMBC and NOESY spectroscopic analyses. The absolute configuration of 1 was elucidated by comparison of the experimental and calculated ECD spectra. The anti-inflammatory effects of 1-7 were evaluated through lipopolysaccharide-induced nitric oxide (NO) production in RAW 264...
July 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28726414/citrifurans-a-d-four-dimeric-aromatic-polyketides-with-new-carbon-skeletons-from-the-fungus-aspergillus-sp
#4
Guo-Ping Yin, Ya-Rong Wu, Ming-Hua Yang, Tian-Xiao Li, Xiao-Bing Wang, Miao-Miao Zhou, Jian-Li Lei, Ling-Yi Kong
Citrifurans A-D (1-4), metabolized by an Aspergillus sp., are unusual dimers of azaphilone and furanone derivatives. Michael addition was thought to be the pivotal procedure in their biosynthesis, and different addition sites generated two new different carbon skeletons. Their structures were elucidated on the basis of spectroscopic methods, single-crystal X-ray diffraction, chemical conversion, and electronic circular dichroism analyses. Compounds 1-3 showed moderate inhibitory activities against LPS-induced NO production in RAW 264...
July 20, 2017: Organic Letters
https://www.readbyqxmd.com/read/28725904/cellular-antioxidant-methylglyoxal-trapping-and-anti-inflammatory-activities-of-cocoa-tea-camellia-ptilophylla-chang
#5
Xiong Gao, Xiaorong Lin, Xiaofei Li, Yuanyuan Zhang, Zhongzheng Chen, Bin Li
Cocoa tea (Camellia ptilophylla Chang) is a naturally low caffeine-containing but gallocatechin gallate (GCG)-rich tea cultivar, though its biological activities have not been extensively explored. Herein, we evaluated the in vitro cellular antioxidant, methylglyoxal trapping, and anti-inflammatory activities of water extract of green tea from cocoa tea (CWE) and Yunnan Daye tea (Camellia sinensis) (YWE), and their predominant bioactive components GCG and epigallocatechin gallate (EGCG) for comparative purposes...
July 20, 2017: Food & Function
https://www.readbyqxmd.com/read/28719189/determination-of-double-bond-positions-in-polyunsaturated-fatty-acids-using-the-photochemical-patern%C3%A3-b%C3%A3-chi-reaction-with-acetone-and-tandem-mass-spectrometry
#6
Robert C Murphy, Toshiaki Okuno, Christopher A Johnson, Robert M Barkley
The position of double bonds along the carbon chain of methylene interrupted polyunsaturated fatty acids are unique identifiers of specific fatty acids derived from biochemical reactions that occur in cells. It is possible to obtain direct structural information as to these double bond positions using tandem mass spectrometry after collisional activation of the carboxylate anions of an acetone adduct at each of the double bond positions formed by the photochemical Paternò-Büchi reaction with acetone. This reaction can be carried out by exposing a small portion of an inline fused silica capillary to UV photons from a mercury vapor lamp as the sample is infused into the electrospray ion source of a mass spectrometer...
July 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28715912/acetyl-and-o-alkyl-derivatives-of-%C3%AE-mangostin-from-garcinia-mangostana-and-their-anti-inflammatory-activities
#7
Thiruventhan Karunakaran, Gwendoline Cheng Lian Ee, Intan Safinar Ismail, Siti Mariam Mohd Nor, Nor Hisam Zamakshshari
Pure β-mangostin (1) was isolated from the stem bark of Garcinia mangostana L. One monoacetate (2) and five O-alkylated β-mangostin derivatives (3-7) were synthesised from β-mangostin. The structures of these compounds were elucidated and determined using spectroscopic techniques such as 1D NMR and MS. The cytotoxicities and anti-inflammatory activities of these five compounds against RAW cell 264.7 were tested. The structural-activity relationship studies indicated that β-mangostin showed a significant activity against the LPS-induced RAW cell 264...
July 17, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28715873/subcritical-water-hydrolyzed-fish-collagen-ameliorates-survival-of-endotoxemic-mice-by-inhibiting-hmgb1-release-in-a-ho-1-dependent-manner
#8
Min Young Ahn, Jung Seok Hwang, Sun Ah Ham, Jinwoo Hur, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Sung Gu Han, Han Geuk Seo
To investigate potential mechanisms underlying the bioactivity of hydrolyzed fish collagen, we examined the anti-inflammatory actions of subcritical water-hydrolyzed fish collagen (SWFC) in lipopolysaccharide (LPS)-triggered inflammation and endotoxemia. SWFC markedly inhibited LPS-stimulated release of high mobility group box 1 (HMGB1) in murine RAW264.7 macrophages, along with decreased cytosolic translocation of HMGB1. Both the protein and mRNA levels of heme oxygenase-1 (HO-1) were significantly upregulated in SWFC-treated RAW 264...
July 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28713364/immunomodulatory-activities-of-a-fungal-protein-extracted-from-hericium-erinaceus-through-regulating-the-gut-microbiota
#9
Chen Diling, Zheng Chaoqun, Yang Jian, Li Jian, Su Jiyan, Xie Yizhen, Lai Guoxiao
A single-band protein (HEP3) was isolated from Hericium erinaceus using a chemical separation combined with pharmacodynamic evaluation methods. This protein exhibited immunomodulatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by decreasing the overproduction of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, and downregulating the expression of inducible nitric oxide synthase and nuclear factor-κB p65. Further researches revealed that HEP3 could improve the immune system via regulating the composition and metabolism of gut microbiota to activate the proliferation and differentiation of T cells, stimulate the intestinal antigen-presenting cells in high-dose cyclophosphamide-induced immunotoxicity in mice, and play a prebiotic role in the case of excessive antibiotics in inflammatory bowel disease model mice...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28712894/antimicrobial-and-immunomodulatory-activity-of-host-defense-peptides-clavanins-and-ll-37-in-vitro-an-endodontic-perspective
#10
Stella Maris F Lima, Mirna S Freire, Ana Luisa O Gomes, Ana Paula C Cantuária, Flávia Rodrigues P Dutra, Beatriz S Magalhães, Maurício Gonçalves C Sousa, Ludovico Migliolo, Jeeser A Almeida, Octávio L Franco, Taia Maria B Rezende
Endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. Endodontic therapy can be benefitted by host defense peptides (HDPs), which are multifunctional molecules that act against persistent infection and inflammation. This study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several HDPs, namely clavanin A, clavanin A modified (MO) and LL-37, compared to intracanal medication Ca(OH)2. HDPs and Ca(OH)2 were evaluated by: (1) antimicrobial assays against Candida albicans and Enterococcus faecalis, (2) cytotoxicity assays and (3) cytokine tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP)-1, interleukin (IL)-1α, IL-6, IL-10 and IL-12 and nitric oxide (NO) production by RAW 264...
July 14, 2017: Peptides
https://www.readbyqxmd.com/read/28711615/water-soluble-polysaccharide-from-erythronium-sibiricum-bulb-structural-characterisation-and-immunomodulating-activity
#11
Rena Kasimu, Chunli Chen, Xiangyun Xie, Xue Li
A water-soluble polysaccharide named ESBP2-1 was isolated from Erythronium sibiricum bulb through anion-exchange and size-exclusion chromatography. ESBP2-1 is a neutral polysaccharide fraction with an average molecular weight of 9.4×10(5)Da and is composed of glucose, galactose and arabinose in a ratio of 24.3:1.1:1. Methylation analysis, partial hydrolysis and NMR studies revealed that the backbone of ESBP2-1 primarily consists of repeating →1)-α-D-Glcp-(4→ units where the disaccharide side chains of Arap-(1→6)-α-D-Galp-(1→ residue are attached to the O-6 position of Glc...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28708322/ganglioside-gm3-suppresses-lipopolysaccharide-induced-inflammatory-responses-in-raw-264-7-macrophage-cells-through-nf-%C3%AE%C2%BAb-ap-1-and-mapks-signaling
#12
Junyoung Park, Choong-Hwan Kwak, Sun-Hyung Ha, Kyung-Min Kwon, Fukushi Abekura, Seung-Hak Cho, Young-Chae Chang, Young-Choon Lee, Ki-Tae Ha, Tae-Wook Chung, Cheorl-Ho Kim
Gangliosides are known to specifically inhibit vascular leukocyte recruitment and consequent interaction with the injured endothelium, the basic inflammatory process. In this study, we have found that the production of nitric oxide (NO), a main regulator of inflammation, is suppressed by GM3 on murine macrophage RAW264.7 cells, when induced by LPS. In addition, GM3 attenuated the increase in cyclooxyenase-2 (COX-2) protein and mRNA levels in lipopolysaccharide (LPS)-activated RAW 264.7 cells in a dose-dependent manner...
July 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28707226/the-role-of-macrophage-polarization-on-fibroblast-behavior-an-in-vitro-investigation-on-titanium-surfaces
#13
Xuzhu Wang, Yulan Wang, Dieter D Bosshardt, Richard J Miron, Yufeng Zhang
OBJECTIVES: This study investigated the effect of smooth and rough titanium surface topographies on macrophage polarization and their influence on gingival fibroblast behavior cultured on titanium surfaces. MATERIALS AND METHODS: RAW 264.7 macrophages were seeded on smooth (pickled titanium (PT)) and rough Sand-blasted with Large grit particles followed by Acid-etching (SLA) titanium surfaces and first investigated for macrophage polarization towards tissue-inflammatory M1 macrophages or wound-healing M2 macrophages...
July 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28700941/probing-mammalian-cell-size-homeostasis-by-channel-assisted-cell-reshaping
#14
Giulia Varsano, Yuedi Wang, Min Wu
Cell size homeostasis can be achieved by size checkpoints that couple cell size to cell-cycle progression or by alternative mechanisms such as constant extension. In mammalian cells, the existence of strict size checkpoints remains controversial due to the technical limitations in determining cell size directly and accurately. We developed a microfabricated channel system that linearizes mammalian cell growth and facilitates cell size measurements. By tracking cell length, while directly visualizing cell-cycle progression in rat basophilic leukemia cells and RAW 264...
July 11, 2017: Cell Reports
https://www.readbyqxmd.com/read/28700722/anti-inflammatory-properties-of-extracts-from-chimonanthus-nitens-oliv-leaf
#15
Qi Sun, Jiajin Zhu, Feiwei Cao, Fengjia Chen
Chimonanthus nitens Oliv. (CN) is a species in the family Calycanthaceae. Its leaf is widely used to make traditional herbal tea in southern China and has a wide range of therapeutic effects. The profile of the ethanol extracts from CN leaves was identified by UPLC-QTOF-MS/MS. Forty seven compounds were determined including organic acids, phenolic acids and derivatives, flavonoids, coumarins, fatty acids and other compounds. The effect of the CN extracts on the inflammatory damage in zebrafish and in RAW 264...
2017: PloS One
https://www.readbyqxmd.com/read/28699786/inactivation-of-norovirus-by-lemongrass-essential-oil-using-a-norovirus-surrogate-system
#16
Ye Won Kim, Hyun Ju You, Soyoung Lee, Bomi Kim, Do Kyung Kim, Joo-Bong Choi, Ji-Ah Kim, Hee Jung Lee, In Sun Joo, Jeong Su Lee, Dong Hyun Kang, Giljae Lee, Gwang Pyo Ko, Sung-Joon Lee
This study investigated the effect of lemongrass essential oil (LGEO) on the infectivity and viral replication of norovirus. Murine norovirus 1 (MNV-1), a surrogate of human norovirus, was preincubated with LGEO and then used to infect RAW 264.7 cells in a plaque reduction assay. LGEO exhibited a significant reduction in MNV-1 plaque formation in both time- and dose-dependent manners. The quantification of viral genome by quantitative real-time PCR showed similar results in line with those of the plaque reduction assay...
July 12, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28698676/new-anti-inflammatory-metabolites-produced-by-streptomyces-violaceoruber-isolated-from-equus-burchelli-feces
#17
Jian Ma, Hui Lei, Xiu Chen, Xiaoxu Bi, Yi Jiang, Li Han, Xueshi Huang
Three new metabolites (2-4), together with one known compound, GTRI-02, (1) were isolated from a fermentation broth of Streptomyces violaceoruber derived from Equus burchelli feces. The structures of the new compounds 2-4 were established using comprehensive NMR spectroscopic data analysis as well as UV, IR and MS data. The anti-inflammatory activity of compounds 1-4 was tested by examining their ability to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells...
July 12, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28698461/dereplication-guided-isolation-of-new-phenylpropanoid-substituted-diglycosides-from-cistanche-salsa-and-their-inhibitory-activity-on-no-production-in-macrophage
#18
Jongmin Ahn, Hee-Sung Chae, Young-Won Chin, Jinwoong Kim
Dereplication allows for a rapid identification of known and unknown compounds in plant extracts. In this study, we performed liquid chromatography-mass spectroscopy (LC-MS)- based dereplication using data from ESI⁺ QTOF-MS for the analysis of phenylpropanoid-substituted diglycosides, the major active constituents of Cistanche salsa (C. A. Mey.) Beck. Using TOF-MS alone, the substructures of these compounds could be unambiguously confirmed based on the characteristic fragmentation patterns of various product ions...
July 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28697633/zinc-l-carnosine-suppresses-inflammatory-responses-in-lipopolysaccharide-induced-raw-264-7-murine-macrophages-cell-line-via-activation-of-nrf2-ho-1-signaling-pathway
#19
Theng Choon Ooi, Kok Meng Chan, Razinah Sharif
CONTEXT: Zinc L-carnosine (ZnC) is a chelate of Zn and L-carnosine and is used clinically in the treatment of peptic ulcer. OBJECTIVE: In this study, we aim to investigate the involvement of heme oxygenase-1 (HO-1) in the anti-inflammatory effects of ZnC in lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophages. MATERIALS AND METHODS: We used immunoblotting analysis to evaluate the involvement of HO-1 in the anti-inflammatory effects of ZnC and the signaling pathway involved was measured using Dual luciferase reporter assay...
July 11, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28695725/highly-selective-and-sensitive-two-photon-fluorescence-probe-for-endogenous-peroxynitrite-detection-and-its-applications-in-living-cells-and-tissues
#20
Jun Li, Chang Su Lim, Gyoungmi Kim, Hwan Myung Kim, Juyoung Yoon
A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO(-)) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO(-) in phosphate-buffered saline solution with a low detection limit (3.5 × 10(-8) M). Furthermore, the probe displays good performance in detecting endogenous ONOO(-), not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.
July 24, 2017: Analytical Chemistry
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