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Raw 264.7

Wen-Juan Li, Yong Wang, Yulin Liu, Teng Wu, Wen-Li Cai, Xin-Tao Shuai, Guo-Bin Hong
Purpose: To establish small-sized superparamagnetic polymeric micelles for magnetic resonance and fluorescent dual-modal imaging, we investigated the feasibility of MR imaging (MRI) and macrophage-targeted in vitro. Methods: A new class of superparamagnetic iron oxide nanoparticles (SPIONs) and Nile red-co-loaded mPEG-Lys3-CA4-NR/SPION polymeric micelles was synthesized to label Raw264.7 cells. The physical characteristics of the polymeric micelles were assessed, the T2 relaxation rate was calculated, and the effect of labeling on the cell viability and cytotoxicity was also determined in vitro...
2018: International Journal of Medical Sciences
Xue-Yan Yang, Yi-Fan Zhang, Li-Jia Liu, Yi Wang, Ming-Ying Shang, Feng Xu, Guang-Xue Liu, Shao-Qing Cai
A new lignan, tirpitzin A (17) together with 20 known compounds (1-16, and 18-21) were isolated from the ethyl acetate soluble fraction of ethanol extract of the aerial parts of Tirpitzia ovoidea. The structure of new compound was elucidated by means of spectroscopic analysis. Of the known compounds, 7-21 were isolated from Linaceae family for the first time. The pharmacological activity of the crude extracts was tested using a mouse inflammation model induced by dimethyl benzene. The results demonstrated that the ethyl acetate soluble fraction had anti-inflammatory activity...
December 2017: Chinese Journal of Natural Medicines
Jia Xiong, Mary H Grace, Debora Esposito, Slavko Komarnytsky, Fei Wang, Mary Ann Lila
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264...
November 2017: Chinese Journal of Natural Medicines
Hien Thi Thu Le, Young-Chang Cho, Sayeon Cho
Guettarda speciosa Linn. (G. speciosa, Rubiaceae) has been used as a traditional medicinal plant in Asia for the treatment of various inflammatory conditions, including cough, fever and maternal postpartum infection. However, the mechanisms underlying the anti‑inflammatory action of G. speciosa extracts have remained elusive. In the present study, the anti‑inflammatory effects of the methanol extract of G. speciosa (MGS) were investigated in murine macrophages by measuring the production of inflammatory mediators and the underlying mechanisms of action by performing immunoblotting analysis of proteins that are potentially involved...
January 10, 2018: International Journal of Molecular Medicine
Seock-Jin Chung, Hai-Jeon Yoon, Gi Jeong Cheon, Hyewon Youn, Mi Jeong Kim, Lin Xie, Yun-Sang Lee, Jae Min Jeong, June-Key Chung, Keon Wook Kang, Ming-Rong Zhang
Activated macrophages have been known to play pivotal roles in the pathogenesis of rheumatoid arthritis (RA). 18F-FEDAC is a radiolabeled ligand for the translocator protein (TSPO), which is abundant in activated macrophages. We evaluated the feasibility of 18F-FEDAC in a murine RA model. Methods: RAW 264.7 mouse macrophages were activated by lipopolysaccharide. TSPO expression levels in activated and inactivated macrophages were measured by quantitative polymerase chain reaction (qPCR) and western blotting...
January 11, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Xiaokai Li, Siyuan Feng, Yi Luo, Keren Long, Zhenghao Lin, Jideng Ma, Anan Jiang, Long Jin, Qianzi Tang, Mingzhou Li, Xun Wang
Macrophage-derived foam cells were one of the hallmarks of atherosclerosis, and microRNAs played an important role in the formation of foam cells. In order to explore the roles of miRNA in the formation of foam cells, we investigated miRNA expression profiles in foam cells through high-throughput sequencing technology. A total of 84 miRNAs were differentially expressed between RAW 264.7 macrophages and foam cells induced by ox-LDL. Thirty miRNAs were upregulated and 54 miRNAs were downregulated. GO terms and KEGG pathways analysis revealed that the target genes of most of DE miRNAs were mainly enriched in "cell differentiation," "endocytosis," "MAPK signaling pathway," and "FoxO signaling pathway...
January 10, 2018: In Vitro Cellular & Developmental Biology. Animal
Venkata Harini Kema, Imran Khan, Suman Kapur, Palash Mandal
Diallyl sulfide (DAS) has been studied extensively for its alleged role as an anticancer and protective agent. Alcohol influences and effects on human health have been extensively studied. However, investigations toward developing and testing therapeutic agents that can reduce the tissue injury caused by ethanol are scarce. In this backdrop, this study was designed to explore the potential effect of DAS in reducing alcohol induced damage of 3T3L1 adipocytes and RAW 264.7 macrophages. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was performed to determine the DAS effect on cell viability...
January 10, 2018: Drug and Chemical Toxicology
Xianjun Sun, Xiaohong Deng, Waijiao Cai, Wenwei Li, Ziyin Shen, Tinghui Jiang, Jianhua Huang
AIMS: Icariin (ICA) is a flavonoid isolated from certain plant species in the genus Epimedium, especially Epimedium brevicornum. Previous studies indicated that ICA has certain regulatory effects on some inflammatory diseases, and that ICA regulates the activity of glucocorticoid receptor (GR) and NF-κB. But the causal link between GR and NF-κB and other downstream pathways in effects of ICA remained elusive, therefore here we have investigated whether ICA could promote GR function, in turn, to regulate NF-κB and/or other factors to achieve its anti-inflammatory effect...
January 6, 2018: Life Sciences
M B Lapenkova, N S Smirnova, P N Rutkevich, M A Vladimirsky
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium...
January 2018: Bulletin of Experimental Biology and Medicine
Zhiqiang Li, Hui Zhang, Jinliang Zhang, Li Xi, Guangli Yang, Shuli Wang, Qingfeng Zhou, Xiaogen Zhang, Junbo Zhang
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins...
January 4, 2018: World Journal of Microbiology & Biotechnology
Hyun Gyung Kim, Sang Hoon Kim, Taek-Seung Kim, Tae Won Park, Ran Won, Hee-Deung Park, Soo An Choi, Yong Woo Jung
Carbon nanotubes (CNTs) are nanomaterials that have been employed in generating diverse materials. We previously reported that CNTs induce cell death in macrophages, possibly via asbestosis. Therefore, we generated CNT-attached polyvinylidene fluoride (PVDF), which is an established polymer in membrane technology, and then examined whether CNT-attached PVDF is immunologically safe for medical purposes compared to CNT alone. To test this, we treated RAW 264.7 murine macrophages (RAW cells) with CNT-attached PVDF and analyzed the production of nitric oxide (NO), a potent proinflammatory mediator, in these cells...
December 2017: Immune Network
Chang-Ju Kim, Sang-Hun Shin, Bok-Joo Kim, Chul-Hoon Kim, Jung-Han Kim, Hae-Mi Kang, Bong-Soo Park, In-Ryoung Kim
Kaempferol, a flavonoid compound, is derived from the rhizome of Kaempferia galanga L., which is used in traditional medicine in Asia. Autophagy has pleiotropic functions that are involved in cell growth, survival, nutrient supply under starvation, defense against pathogens, and antigen presentation. There are many studies dealing with the inhibitory effects of natural flavonoids in bone resorption. However, no studies have explained the relationship between the autophagic and inhibitory processes of osteoclastogenesis by natural flavonoids...
January 2, 2018: International Journal of Molecular Sciences
Jennifer D Sisler, Chaolong Qi, Walter McKinney, Justine Shaffer, Michael Andrew, Taekhee Lee, Treye Thomas, Vincent Castranova, Robert R Mercer, Yong Qian
To protect against decay and fungal invasion into the wood, the micronized copper, copper carbonate particles, has been applied in the wood treatment in recent years; however, there is little information on the health risk associated with sanding micronized copper- treated lumber. In this study, wood dust from the sanding of micronized copper azole -treated lumber (MCA) was compared to sanding dust from solubilized copper azole-treated wood (CA-C) and untreated yellow pine (UYP). The test found that sanding MCA released a much higher concentration of nanoparticles than sanding CA-C and UYP, and the particles between about 0...
January 4, 2018: Journal of Occupational and Environmental Hygiene
Da-Peng Qin, Da-Bo Pan, Wei Xiao, Hai-Bo Li, Biao Yang, Xiao-Jun Yao, Yi Dai, Yang Yu, Xin-Sheng Yao
Arteannoide A (1), an unusual cadinane dimer featuring a rare fused 6,8-dioxabicyclo[3.2.l]octan-7-one ring system, arteannoides B and C (2 and 3), two novel heterodimers incorporating a rearranged cadinene sesquiterpenoid and a phenylpropanoid, together with two new rearranged cadinene sesquiterpenoids 4 and 5, were isolated from Artemisia annua L. Their structures were determined by a combination of NMR spectroscopy, electronic circular dichroism calculations, and X-ray diffraction analyses. Compounds 2 and 3 showed inhibition of nitric oxide production in lipopolysaccharide-induced RAW 264...
January 4, 2018: Organic Letters
Vilasinee Hirunpanich Sato, Bunleu Sungthong, Prasob-Orn Rinthong, Narawat Nuamnaichati, Supachoke Mangmool, Savita Chewchida, Hitoshi Sato
CONTEXT: Chatuphalatika (CTPT), is a Thai herbal formulation mixture of Phyllanthus emblica Linn. (Euphorbiaceae), Terminalia belerica Linn. (Combretaceae), T. chebula and the fruit of T. arjuna (Roxb.) Wight & Arn. CTPT is considered to exert anti-inflammatory and antihyperuricemic effects, but there have been no reports to demonstrate these pharmacological effects in a quantitative manner. OBJECTIVES: To investigate the antioxidative, anti-inflammatory and antihyperuricemic effects of CTPT...
December 2018: Pharmaceutical Biology
Suvarna H Pagire, Eunhye Lee, Haushabhau S Pagire, Eun Jung Bae, Soo Jung Ryu, Dahye Lee, Min Hee Kim, Geum Ran Kim, Kyu-Seok Hwang, Sukyung Ahn, Jin Hee Maeng, Jin Sook Song, Myung Ae Bae, Don Hang Lee, Jin Hee Ahn
A series of glutamic acid derivatives was synthesized and evaluated for their antioxidant activity and stability. We found several potent and stable glutamic acid derivatives. Among them, compound 12b exhibited good in vitro activity, chemical stability and cytotoxicity. A prototype compound 12b showed an anti-inflammatory effect in LPS-stimulated RAW 264.7 cell lines and in a zebrafish model.
November 7, 2017: Bioorganic & Medicinal Chemistry Letters
Diane M Spencer, John R Dye, Claude A Pianatadosi, David S Pisetsky
Microparticles (MPs) are small membrane-bound vesicles released from activated or dying cells. As shown previously, LPS stimulation of the RAW 264.7 macrophage cell line can induce MP release, with the caspase inhibitor Z-VAD increasing the extent of this process. Since combined treatment of cells with LPS and Z-VAD can induce necroptosis, we explored particle release during this form of cell death using flow cytometry to assess particle size, binding of annexin V and staining for DNA with propidium iodide (PI) and SYTO 13...
December 29, 2017: Experimental Cell Research
Jingwen Zhang, Anaïs Pitto-Barry, Lijun Shang, Nicolas P E Barry
We report an evaluation of the cytotoxicity of a series of electron-deficient (16-electron) half-sandwich precious metal complexes of ruthenium, osmium and iridium ([Os/Ru(η6-p-cymene)(1,2-dicarba-closo-dodecarborane-1,2-dithiolato)] (1/2), [Ir(η5-pentamethylcyclopentadiene)(1,2-dicarba-closo-dodecarborane-1,2-dithiolato)] (3), [Os/Ru(η6-p-cymene)(benzene-1,2-dithiolato)] (4/5) and [Ir(η5-pentamethylcyclopentadiene)(benzene-1,2-dithiolato)] (6)) towards RAW 264.7 murine macrophages and MRC-5 fibroblast cells...
November 2017: Royal Society Open Science
Yu-Ra Kang, Joon Park, Sung Keun Jung, Yoon Hyuk Chang
The aims of this study were to synthesize chlorophyll derivatives, pheophytins and Zn-pheophytins, from chlorophylls extracted from spinach, characterize them, and evaluate their antioxidant and anti-inflammatory activities. The chlorophylls isolated from spinach were identified by means of FT-IR and NMR spectroscopies. The synthesis of pheophytins and Zn-pheophytins was confirmed by UV-Vis spectral analyses. The antioxidant activity of chlorophylls, pheophytins, and Zn-pheophytins was studied. The results revealed that the Zn-pheophytins showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and β-carotene bleaching activities, followed by chlorophylls and pheophytins...
April 15, 2018: Food Chemistry
Tiane C Finimundy, Lillian Barros, Ricardo C Calhelha, Maria José Alves, Miguel A Prieto, Rui M V Abreu, Aldo J P Dillon, João A P Henriques, Mariana Roesch-Ely, Isabel C F R Ferreira
A study with Pleurotus sajor-caju was conducted to: evaluate the nutritional and chemical composition of the fruiting bodies; optimize the preparation of bioactive phenolic extracts; and characterize the optimized extract in terms of bioactive compounds and properties. P. sajor-caju revealed an equilibrated nutritional composition with the presence of hydrophilic (sugars and organic acids) and lipophilic (tocopherols and PUFA) compounds. p-Hydroxybenzoic, p-coumaric and cinnamic acids were identified in the extract obtained with ethanol (30g/l ratio) at 55°C for 85min...
April 15, 2018: Food Chemistry
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