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https://www.readbyqxmd.com/read/29784301/two-new-farnesyl-phenolic-compounds-with-anti-inflammatory-activities-from-ganoderma-duripora
#1
Jie-Qing Liu, Chen-Lei Lian, Tian-Yong Hu, Cui-Fang Wang, Ying Xu, Lei Xiao, Zhi-Qiang Liu, Shu-Qi Qiu, Bao-Hui Cheng
Ganoderma fungi have long been used as a famous traditional medicine and food in country of East Asia. In this work, two new farnesyl phenolic compounds, ganoduriporols A and B (1 and 2), were isolated from the fruiting bodies of Ganoderma duripora, and their structures were elucidated using various spectroscopic methods. Anti-inflammatory activities were assayed and evaluated for the two compounds. Ganoduriporols A and B exhibited dose-dependent anti-inflammatory effects in RAW 264.7 cells. Furthermore, ganoduriporol A was demonstrated to inhibit the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and prostaglandin E2 (PGE2 ) through the suppression of COX-2, MAPK and NF-κB signaling pathway in LPS-induced macrophage cells...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29784022/in-vitro-screening-of-known-drugs-identified-by-scaffold-hopping-techniques-shows-promising-leishmanicidal-activity-for-suramin-and-netilmicin
#2
Supriya Khanra, Y Pavan Kumar, Jyotirmayee Dash, Rahul Banerjee
OBJECTIVE: The rapid emergence of drug resistant Leishmanial strains makes it imperative to continue the development of cheap and effective drugs against the parasite. Due to the absence of effective vaccines against leishmaniasis, current therapeutic measures exclusively rely on chemotherapy. Here we attempt, to identify novel antileishmanial from a list of known drugs determined from a previous bioinformatics study. Synergism between various drug combinations (involving netilmicin, suramin, paromomycin and curcumin) have been estimated to identify potent multidrug therapies to combat the disease...
May 21, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29783150/electrophoretic-deposition-of-dexamethasone-loaded-gelatin-nanospheres-chitosan-coating-and-its-dual-function-in-anti-inflammation-and-osteogenesis
#3
Hongfei Qi, Qiang Chen, Hailong Ren, Xianglong Wu, Xianhu Liu, Tingli Lu
Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration. The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX). GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method. Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD)...
May 18, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29783120/canine-mesenchymal-stem-cells-treated-with-tnf-%C3%AE-and-ifn-%C3%AE-enhance-anti-inflammatory-effects-through-the-cox-2-pge-2-pathway
#4
Hye-Mi Yang, Woo-Jin Song, Qiang Li, Su-Yeon Kim, Hyeon-Jin Kim, Min-Ok Ryu, Jin-Ok Ahn, Hwa-Young Youn
Mesenchymal stem cells (MSCs) have been used in studies on treatment of various diseases, and their application to immune-mediated diseases has garnered interest. Various methods for enhancing the immunomodulation effect of human MSCs have been used; however, similar approaches for canine MSCs are relatively unexplored. Accordingly, we evaluated immunomodulatory effects and mechanisms in canine MSCs treated with TNF-α and IFN-γ. Lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were incubated with the conditioned media (CM) from canine MSCs for 48 h...
May 14, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29783109/anti-inflammatory-potential-of-alginic-acid-from-sargassum-horneri-against-urban-aerosol-induced-inflammatory-responses-in-keratinocytes-and-macrophages
#5
I P Shanura Fernando, Thilina U Jayawardena, K K Asanka Sanjeewa, Lei Wang, You-Jin Jeon, Won Woo Lee
The airborne particulate pollutants originating in the deserts of Mongolia and China which becomes contaminated with industrial effluents and traffic emissions while moving with the wind currents towards East Asia has recently become a serious environmental and health issue in the region. They cause asthma, collateral lung tissue damage, oxidative stress, allergic reactions, and inflammation. The current study was undertaken to evaluate the protective effects of alginate extracted from the invasive alga Sargassum horneri (SHA) against fine dust collected from Beijing, China (Chinese fine dust; CFD)...
May 18, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29777902/chemical-characterization-and-cerebroprotective-effect-of-methanolic-root-extract-of-colebrookea-oppositifolia-in-rats
#6
Gollapalle Lakshminarayanashastry Viswanatha, Marikunte V Venkataranganna, Nunna Bheema Lingeswara Prasad, Shylaja Hanumanthappa
ETHNOPHARMACOLOGICAL RELEVANCE: Colebrookea oppositifolia Smith is one of the extensively used plants to treat neurological conditions such as epilepsy by the various ethnic communities in sub-Himalayan regions of India such as Bhoxa, Tharu and nomadic Gujjars. AIM OF THE STUDY: This study was conducted to evaluate the cerebroprotective effect of C. oppositifolia methanolic root (MeCO) extract in Wistar rats. MATERIALS AND METHODS: The MeCO was characterized for total phenolic content and later subjected for detailed liquid chromatography-mass spectrometry analysis...
May 16, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29776311/phospholipid-block-copolymer-hybrid-vesicles-with-lysosomal-escape-ability
#7
Wei Zong, Bo Thingholm, Fabian Itel, Philipp Sebastian Schattling, Edit Brodszkij, Daniel Mayer, Steffen Stenger, Kenneth N Goldie, Xiaojun Han, Brigitte Städler
The success of nanoparticulate formulations in drug delivery depends on various aspects including their toxicity, internalization and intracellular location. Vesicular assemblies consisting of phospholipid and amphiphilic block copolymers are an emerging platform, which combines the benefits from liposomes and polymersomes while overcoming their challenges. We report the synthesis of poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino) ethyl methacrylate) (pCMA-b-pDMAEMA) block copolymers and their assembly with phospholipids into hybrid vesicles...
May 18, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29775898/particle-induced-sirt1-downregulation-promotes-osteoclastogenesis-and-osteolysis-through-er-stress-regulation
#8
Liang Zhang, Dongmei Bao, Peng Li, Zhidong Lu, Long Pang, Zhirong Chen, Haohui Guo, Zhihui Gao, Qunhua Jin
BACKGROUND: Sirtuin 1 (SIRT1) downregulation has been found to be induced by wear particles in aseptic prosthesis loosening (APL). Osteoclastogenesis and osteoclast activation are the main pathological factors associated with APL. However, whether SIRT1 downregulation contributes to the formation and activation of osteoclasts through the induction of endoplasmic reticulum (ER) stress is unclear. METHODS: To address this, an osteolysis mouse model was used in which animals were treated with the SIRT1 activator, resveratrol (RES), or an ER stress inhibitor, 4-PBA, for two weeks...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29772718/ectromelia-virus-affects-mitochondrial-network-morphology-distribution-and-physiology-in-murine-fibroblasts-and-macrophage-cell-line
#9
Karolina P Gregorczyk, Zbigniew Wyżewski, Joanna Szczepanowska, Felix N Toka, Matylda B Mielcarska, Magdalena Bossowska-Nowicka, Małgorzata Gieryńska, Anna Boratyńska-Jasińska, Justyna Struzik, Marek G Niemiałtowski, Lidia Szulc-Dąbrowska
Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines and a combination of immunofluorescence techniques, confocal and electron microscopy, and flow cytometry to address subcellular changes following infection. Early in infection of L929 fibroblasts and RAW 264...
May 16, 2018: Viruses
https://www.readbyqxmd.com/read/29771951/antitumor-antioxidant-and-anti-inflammatory-activities-of-kaempferol-and-its-corresponding-glycosides-and-the-enzymatic-preparation-of-kaempferol
#10
Jingqiu Wang, Xianying Fang, Lin Ge, Fuliang Cao, Linguo Zhao, Zhenzhong Wang, Wei Xiao
Kaempferol (kae) and its glycosides are widely distributed in nature and show multiple bioactivities, yet few reports have compared them. In this paper, we report the antitumor, antioxidant and anti-inflammatory activity differences of kae, kae-7-O-glucoside (kae-7-O-glu), kae-3-O-rhamnoside (kae-3-O-rha) and kae-3-O-rutinoside (kae-3-O-rut). Kae showed the highest antiproliferation effect on the human hepatoma cell line HepG2, mouse colon cancer cell line CT26 and mouse melanoma cell line B16F1. Kae also significantly inhibited AKT phosphorylation and cleaved caspase-9, caspase-7, caspase-3 and PARP in HepG2 cells...
2018: PloS One
https://www.readbyqxmd.com/read/29771509/extzbox-a-glowing-cyclophane-for-live-cell-imaging
#11
Indranil Roy, Sharan Bobbala, Jiawang Zhou, Minh T Nguyen, Siva Krishna Mohan Nalluri, Yilei Wu, Daniel Ferris, Evan Alexander Scott, Michael R Wasielewski, J Fraser Stoddart
The ideal fluorescent probe for live-cell imaging is bright, non-cytotoxic and can be delivered easily into the living cells in an efficient manner. The design of synthetic fluorophores, having all three of these properties, however, has proved to be challenging. Here, we introduce a simple, yet effective, strategy based on well-established chemistry for designing a new class of fluorescent probes for live-cell imaging. A box-like hybrid cyclophane, namely ExTzBox•4X (6•4X, X = PF6 - , Cl- ), has been synthesized by connecting an extended viologen (ExBIPY) and a dipyridyl thiazolothiazole (TzBIPY) unit in an end-to-end fashion with two p-xylylene ( p-Xy) linkers...
May 17, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29770236/magnoliae-cortex-and-maize-modulate-porphyromonas-gingivalis-induced-inflammatory-reactions
#12
Jae-Yoon Kim, Kyoung-Hwa Kim, Eun-Hye Kwag, Yang Jo Seol, Yong Moo Lee, Young Ku, In-Chul Rhyu
Purpose: The aim of this study was to evaluate the capacity of single and combined applications of the bark of the stems and roots of Magnolia officinalis Rehd. et Wils. (Magnoliae Cortex) and Zea mays L. (maize) to modulate inflammation in RAW 264.7 cells stimulated with Porphyromonas gingivalis . Methods: RAW 264.7 cells were stimulated with P. gingivalis , and Magnoliae Cortex and/or maize was added. Cytotoxicity and the capacity to modulate inflammation were determined with a methylthiazol tetrazolium (MTT) assay, nitrite production, enzyme-linked immunosorbent assay (ELISA), and western blotting...
April 2018: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29769604/macrophage-cytokines-enhance-cell-proliferation-of-normal-prostate-epithelial-cells-through-activation-of-erk-and-akt
#13
Tu Dang, Geou-Yarh Liou
Macrophage infiltrations (inflammation) are associated with prostate disorders such as prostatitis, prostatic hyperplasia and prostate cancer. All prostate disorders have elevated cell proliferation, and are initiated from normal prostate epithelial cells. To date, the mechanism of how macrophages regulate normal prostate epithelial cell proliferation remains largely unknown. Using a 3D co-culture system, we here show that Raw 264.7 macrophages increased cell proliferation of normal prostate epithelial PZ-HPV-7 cells...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769428/myokine-irisin-induced-protection-against-oxidative-stress-in-vitro-involvement-of-heme-oxygenase-1-and-antioxidazing-enzymes-superoxide-dismutase-2-and-glutathione-peroxidase
#14
A I Mazur-Bialy, K Kozlowska, E Pochec, J Bilski, T Brzozowski
Irisin is a recently discovered myokine reported as protective protein released from exercising skeletal muscles. Although myokines were recently reported to possess the antioxidizing properties, the impact of irisin on the functions of macrophages with respect to its anti-inflammatory potential has not been fully elucidated. Here, we determined the ability of irisin to interact with reactive oxygen species (ROS) in RAW 264.7 murine macrophages. The macrophages were pre-incubated with irisin (0 - 50 nM), some of which had undergone additional co-incubation with bacterial lipopolysaccharide (LPS) (100 ng/ml)...
February 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29765866/effects-of-endosulfan-isomers-on-cytokine-and-nitric-oxide-production-by-differentially-activated-raw-264-7-cells
#15
Alexander I Terry, Sandra Benitez-Kruidenier, Gregory K DeKrey
Endosulfan is an organochlorine insecticide comprised of two isomers: endosulfan-α and endosulfan-β. Endosulfan exposure has been shown to elevate some inflammatory factors, such as nitric oxide (NO) and tumor necrosis factor (TNF), in animals or cultures of animal cells. Because the two endosulfan isomers can vary in their biological activities, the goal of this study was to determine if individual endosulfan isomers differentially impact production of NO or TNF by the mouse macrophage cell RAW 264.7 at non-cytotoxic levels...
2018: Toxicology Reports
https://www.readbyqxmd.com/read/29763660/docosahexaenoic-acid-derived-oxidized-lipid-metabolites-modulate-the-inflammatory-response-of-lipolysaccharide-stimulated-macrophages
#16
John P Caron, Jeffrey C Gandy, Jennifer L Brown, Lorraine M Sordillo
Docosahexaenoic acid (DHA) supplementation has demonstrated beneficial effects in a number of inflammatory diseases. Increasingly, important contributions to its favorable effects are attributed downstream metabolites called docosanoids. Herein, we investigated the role of DHA-derived oxidized lipid metabolites on inflammatory mediator expression by RAW 264.7 murine macrophages. Specifically, macrophage incorporation of DHA, and the resultant biosynthesis of selected pro-resolving docosanoids was quantified...
May 12, 2018: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/29761425/genistein-has-the-function-of-alleviating-and-treating-disseminated-intravascular-coagulation-caused-by-lipopolysaccharide
#17
Xueqin Chen, Jingyi Tan, Mengqi Yang, Zhi-Kai Liao, Cheng Lu, Youwei Huang, Liang-Cai Wu
Symptoms of disseminated intravascular coagulation (DIC) include thromboembolism, acute attrition bleeding and multiple organ failure. Genistein isolated from leguminous plants has been shown to be effective in oxidation resistance and tumor inhibition. The present study was designed to evaluate the therapeutic effects of genistein in DIC and preliminarily discuss the mechanisms regarding the anti-inflammatory and anticoagulant effect of genistein. Swiss mice were randomly divided into the following groups-(1) lipopolysaccharide (LPS), (2) genistein, (3) dimethyl sulfoxide (DMSO, the non-major solvent component of genistein), (4) DMSO + LPS, (5) saline control group, and (6) heparin control group...
May 14, 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29761397/quantitative-phagocytosis-assays-in-primary-and-cultured-macrophages
#18
Fernando Montaño, Sergio Grinstein, Roni Levin
This chapter describes methods to induce and quantify phagocytosis in primary macrophages and in myeloid cell lines. To this end, we initially detail the isolation of primary human monocytes and their differentiation into macrophages. Because primary cells are comparatively refractory to molecular manipulation, we also describe the culture of RAW 264.7 cells-an immortalized monocyte/macrophage cell line, which is more tractable. The chapter also includes methods for preparation of phagocytic targets, specifically sheep erythrocytes opsonized with immunoglobulin G (IgG), as well as means of distinguishing bound from internalized targets, using fluorescently labeled secondary antibodies...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29757247/enrichment-and-purification-of-total-ginkgo-flavonoid-o-glycosides-from-ginkgo-biloba-extract-with-macroporous-resin-and-evaluation-of-anti-inflammation-activities-in-vitro
#19
Lihu Zhang, Tingting Wu, Wei Xiao, Zhenzhong Wang, Gang Ding, Linguo Zhao
In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O -glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O -glycosides among the six resins...
May 13, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29754612/development-of-extremely-stable-dual-functionalized-gold-nanoparticles-for-effective-colorimetric-detection-of-clenbuterol-and-ractopamine-in-human-urine-samples
#20
Turibius Simon, Muthaiah Shellaiah, Perpectual Steffi, Kien Wen Sun, Fu-Hsiang Ko
New glutamic acid (Glu) and polyethylenimine (PE) functionalized ultra-stable gold nanoparticles (PE-Glu-AuNPs) were developed via a simple NaBH4 reduction method. The low toxicity and biocompatibility of PE-Glu-AuNPs were confirmed via an MTT assay in Raw 264.7 cells. Excitingly, PE-Glu-AuNPs were found to be extremely stable at room temperature up to six months and were utilized in an effective colorimetric naked eye assay of clenbuterol (CLB) and ractopamine (RCT) at pH 5. It was found that the selective assay of CLB and RCT is not affected by any other interferences (such as alanine, phenylalanine, NaCl, CaCl2 , threonine, cysteine, glycine, glucose, urea and salbutamol)...
September 6, 2018: Analytica Chimica Acta
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