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Corneal Transplantation

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https://www.readbyqxmd.com/read/28933958/transplantation-of-human-corneal-endothelial-cells-cultured-on-bio-engineered-collagen-vitrigel-in-a-rabbit-model-of-corneal-endothelial-dysfunction
#1
Junko Yoshida, Seiichi Yokoo, Ayumi Oshikata-Miyazaki, Shiro Amano, Toshiaki Takezawa, Satoru Yamagami
PURPOSE: To evaluate the effect of transplanting bioengineered corneal endothelial grafts in a rabbit model of corneal endothelial failure. METHODS: Human corneal endothelial cells (HCECs) were seeded on a vitrigel carrier. After Descemet's membrane was removed from the eyes of rabbits, transplantation was done with a vitrigel/HCEC graft or vitrigel alone without cells, or the eyes were left untreated. Slit lamp examinations and measurement of the central corneal thickness (CCT) were performed for 14 days postoperatively...
September 21, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28932339/penetrating-keratoplasty-for-keratoconus-excimer-versus-femtosecond-laser-trephination
#2
Berthold Seitz, Achim Langenbucher, Tobias Hager, Edgar Janunts, Moatasem El-Husseiny, Nora Szentmáry
BACKGROUND: In case of keratoconus, rigid gas-permeable contact lenses as the correction method of first choice allow for a good visual acuity for quite some time. In a severe stage of the disease with major cone-shaped protrusion of the cornea, even specially designed keratoconus contact lenses are no more tolerated. In case of existing contraindications for intrastromal ring segments, corneal transplantation typically has a very good prognosis. METHODS: In case of advanced keratoconus - especially after corneal hydrops due to rupture of Descemet's membrane - penetrating keratoplasty (PKP) still is the surgical method of first choice...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28928909/transplanting-embryonic-stem-cells-onto-damaged-human-corneal-endothelium
#3
Charles Hanson, Arsaell Arnarsson, Thorir Hardarson, Ann Lindgård, Mandana Daneshvarnaeini, Catarina Ellerström, Anita Bruun, Ulf Stenevi
AIM: To investigate whether human embryonic stem cells (hESCs) could be made to attach, grow and differentiate on a human Descemet's membrane (DM). METHODS: Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis...
August 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/28923582/glaucoma-following-corneal-replacement
#4
REVIEW
Monika Baltaziak, Hall F Chew, Dominik W Podbielski, Iqbal Ike K Ahmed
Glaucoma is a well-known complication following corneal transplantation surgery. Traditional corneal transplantation surgery, specifically penetrating keratoplasty (PK), has been slowly replaced by the advent of new corneal transplantation procedures: primarily lamellar keratoplasties. There has also been an emergence of keratoprosthesis implants for eyes that are high risk of failure with PK. Consequently, there are different rates of glaucoma, pathogenesis, and potential treatment in the form of medical, laser or surgical therapy...
September 16, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28922328/minor-salivary-gland-transplantation-for-severe-dry-eyes
#5
Tais Hitomi Wakamatsu, Ana Estela Besteti Pires Ponce SantʼAnna, Priscila Cardoso Cristovam, Venâncio Avancini Ferreira Alves, Alda Wakamatsu, José Alvaro Pereira Gomes
Dry eye is a multifactorial disease comprising a wide spectrum of ocular surface alterations and symptoms of discomfort. In most patients with aqueous-deficient dry eye, pharmaceutical tear substitutes are used to control symptoms and prevent ocular surface damage. However, in severe dry eye conditions caused by cicatricial disorders, such as Stevens-Johnson syndrome and ocular cicatricial mucous membrane pemphigoid, noninvasive treatments are insufficient, and patients are at risk of developing complications that can lead to blindness...
September 15, 2017: Cornea
https://www.readbyqxmd.com/read/28900769/interface-scopulariopsis-gracilis-fungal-keratitis-following-descemet-s-stripping-automated-endothelial-keratoplasty-dsaek-with-a-contaminated-graft
#6
Craig Wilde, Marco Messina, Tara Moshiri, Susan E Snape, Senthil Maharajan
PURPOSE: To report for the first time a case of interface Scopulariopsis gracilis fungal keratitis following Descemet's stripping automated endothelial keratoplasty (DSAEK) with a contaminated graft. METHODS: A 57-year-old man with bilateral keratoconus and previous bilateral penetrating keratoplasties (PK) developed graft failure in association with marked corneal ectasia. He underwent a successful DSAEK. Unfortunately, a contaminated graft was transplanted and the following morning we were contacted by the eye bank to inform us a slow-growing fungus had been detected in the culture plates inoculated with dextran solution used to store the issued corneoscleral button...
September 12, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28898916/-rehabilitation-following-limbal-stem-cell-deficiency-current-treatment-options-and-future-developments
#7
Henning Thomasen, Klaus P Steuhl, Daniel Meller
Limbal stem cell deficiency (LSCD) is a condition caused by the loss of corneal epithelial regenerative potential. The treatment of this condition is still a challenge. It results from various conditions both intrinsic as well as extrinsic. LSCD can be either uni- or bilateral and either partial or total. Today treatment options include a variety of techniques including transplantation of amniotic membrane and limbal tissue or tissue engineered cell sheets. This article summarizes the current techniques to treat LSCD and upcoming developments...
September 12, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28890079/the-effect-of-chronic-pulmonary-disease-and-mechanical-ventilation-on-corneal-donor-endothelial-cell-density-and-transplant-suitability
#8
Jordan A Margo, Martha F Whiting, Clayton H Brown, Caroline K Hoover, Wuqaas M Munir
PURPOSE: To determine how chronic obstructive pulmonary disease (COPD) and mechanical ventilation time affect corneal donor endothelial cell density (ECD) and transplant suitability. DESIGN: Retrospective cohort study METHODS: Setting: institutional Study Population: 39,679 cornea donor eyes from SightLife Eye Bank between 2012-2016. Demographics, death to preservation time, ECD, lens status, medical history, time on mechanical ventilation, and suitability for transplantation were included...
September 7, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28889066/effect-of-lentivirus-mediated-gene-silencing-targeting-toll-like-receptor-2-on-corneal-allograft-transplantation-in-rats
#9
Lang Bai, Weiyi Liang, Minting Chen, Yacouba Cissé, Jing Liu, Yaru Su, Jian Yu, Qiong Liu
AIM: The present work aims to assess the effectiveness of lentiviral vector (LV) mediated Toll-like receptor 2 (TLR2) gene silencing in the survival of transplanted corneal allografts, against immune rejection, in rats. METHODS: LV mediated TLR2 small interference RNA (SiRNA) with enhanced green fluorescent protein (eGFP) [LV-TLR2-siRNA-eGFP] was realised and transfected to both rat corneal epithelial (EC) and stromal cells (SC). Multiplicity of infection (MOI) was optimized for transfection efficiency using flow cytometric (FCM) analysis...
September 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28885609/vitreous-cavity-length-in-keratoconus-implications-for-keratoplasty
#10
M Messina, T Umapathy, V Avadhanam, C Wilde, D G Said, H S Dua
PurposeTo compare axial length (AL) with vitreous cavity length (VCL) in patients with keratoconus and to ascertain whether graft size can be tailored to reduce myopic refractive error in patients with keratoconus undergoing penetrating keratoplasty (PK).Patients and methodsThe AL and VCL were measured prospectively in patients with keratoconus not undergoing PK (Group 1) and in normal phakic, emmetropic individuals (Group 2). A retrospective analysis of these measurements in patients with keratoconus who had undergone PK (Group 3) was also performed...
September 8, 2017: Eye
https://www.readbyqxmd.com/read/28883661/visualizing-micro-anatomical-structures-of-the-posterior-cornea-with-micro-optical-coherence-tomography
#11
Si Chen, Xinyu Liu, Nanshuo Wang, Xianghong Wang, Qiaozhou Xiong, En Bo, Xiaojun Yu, Shufen Chen, Linbo Liu
Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (µOCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotropic spatial resolution, high image acquisition rate and depth priority scanning mode. In this study, we explored the ability of µOCT to visualize micro-anatomical structures of the posterior cornea ex vivo and in vivo using small and large animals...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28878217/in-vivo-confocal-microscopy-evaluation-of-ocular-surface-with-graft-versus-host-disease-related-dry-eye-disease
#12
Jingliang He, Yoko Ogawa, Shin Mukai, Yumiko Saijo-Ban, Mizuka Kamoi, Miki Uchino, Mio Yamane, Nobuhiro Ozawa, Masaki Fukui, Takehiko Mori, Shinichiro Okamoto, Kazuo Tsubota
Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28873283/in-vivo-like-culture-conditions-in-a-bioreactor-facilitate-improved-tissue-quality-in-corneal-storage
#13
Richard Schmid, Ioana-Sandra Tarau, Angela Rossi, Stefan Leonhardt, Thomas Schwarz, Sebastian Schuerlein, Christian Lotz, Jan Hansmann
The cornea is the most-transplanted tissue worldwide. However, the availability and quality of grafts are limited due to the current methods of corneal storage. In this study, a dynamic bioreactor system is employed to enable the control of intraocular pressure and the culture at the air-liquid interface. Thereby, in vivo-like storage conditions are achieved. Different media combinations for endothelium and epithelium are tested in standard and dynamic conditions to enhance the viability of the tissue. In contrast to culture conditions used in eye banks, the combination of the bioreactor and biochrom medium 1 allows to preserve the corneal endothelium and the epithelium...
September 5, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28855664/oral-mucosal-epithelial-cells-grown-on-porous-silicon-membrane-for-transfer-to-the-rat-eye
#14
Yazad D Irani, Sonja Klebe, Steven J P McInnes, Marek Jasieniak, Nicolas H Voelcker, Keryn A Williams
Dysfunction of limbal stem cells or their niche can result in painful, potentially sight-threatening ocular surface disease. We examined the utility of surface-modified porous-silicon (pSi) membranes as a scaffold for the transfer of oral mucosal cells to the eye. Male-origin rat oral mucosal epithelial cells were grown on pSi coated with collagen-IV and vitronectin, and characterised by immunocytochemistry. Scaffolds bearing cells were implanted into normal female rats, close to the limbus, for 8 weeks. Histology, immunohistochemistry and a multiplex nested PCR for sry were performed to detect transplanted cells...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28844326/-comparison-of-anatomic-and-functional-results-between-z6%C3%A2-femtosecond-laser-assisted-and-manual-trephination-in-deep-anterior-lamellar-keratoplasty-for-advanced-keratoconus-french-version
#15
A Blériot, E Martin, P Lebranchu, K Zimmerman, L Libeau, M Weber, B Vabres, I Orignac
The management of severe keratoconus requires corneal transplantation, for which the gold standard is deep anterior lamellar keratoplasty (DALK), preserving the healthy Descemet's membrane and endothelium. The safety and reproducibility of corneal cuts have been improved by the evolution of femtosecond lasers in refractive surgery, and femtosecond laser in DALK would seem to provide the same advantages over the manual method. In our retrospective study, we compare functional and anatomical results of femtosecond assisted DALK versus manual trephination DALK in patients with keratoconus in stage 4 of the Krumeich classification...
September 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28831627/gender-differences-in-keratoconus-keratoplasty-a-25-year-study-in-southern-brazil-and-global-perspective
#16
Otavio A Magalhaes, Samara B Marafon, Raissa C Ferreira
PURPOSE: To compare the characteristics and outcomes of male and female patients that underwent corneal transplantation for keratoconus (KC) in Southern Brazil and worldwide. METHODS: Retrospective longitudinal study that evaluated medical records of patients who were submitted to keratoplasty between August 1990 and September 2015 in two tertiary hospitals in the state of Rio Grande do Sul (Brazil) and review of the Medline and Embase database international literature...
August 22, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28827194/midterm-outcomes-of-penetrating-keratoplasty-after-cultivated-oral-mucosal-epithelial-transplantation-in-chemical-burn
#17
Alireza Baradaran-Rafii, Siamak Delfazayebaher, Nasser Aghdami, Ehsan Taghiabadi, Shahram Bamdad, Danial Roshandel
PURPOSE: To evaluate the mid-term outcome of penetrating keratoplasty (PKP) after cultivated oral mucosal epithelial transplantation (COMET) in patients with bilateral total limbal stem cell deficiency (LSCD) due to chemical burn. METHODS: In this prospective interventional nonrandomized case series, optical PKP was performed in patients with severe stromal opacity after successful COMET. Main outcome measures were stability of the ocular surface, visual acuity improvement and corneal graft survival...
August 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28820150/component-corneal-surgery-an-update
#18
REVIEW
Prafulla K Maharana, Pranita Sahay, Deepali Singhal, Itika Garg, Jeewan S Titiyal, Namrata Sharma
Several decades ago, penetrating keratoplasty was a challenge to corneal surgeons. Constant effort by the corneal surgeon to improve the outcomes as well as utilization of the available resources has led to a revolutionary change in the field of keratoplasty. All these efforts have led to the evolution of techniques that allow a corneal surgeon to disease-specific transplant of individual layers of corneal "so-called component corneal surgery" depending on the layer of cornea affected. This has led to an improvement in corneal graft survival as well as a better utilization of corneal tissues...
August 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#19
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
August 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28810564/clinical-efficacy-of-conjunctival-flap-surgery-in-the-treatment-of-refractory-fungal-keratitis
#20
Honorine Nizeyimana, Dan-Dan Zhou, Xiu-Fen Liu, Xiao-Tao Pan, Cong Liu, Cheng-Wei Lu, Ji-Long Hao
The aim of the present study was to investigate the use and effectiveness of a selective, partial, pedunculated (tongue-shaped) conjunctival flap (CF) for the treatment of refractory fungal keratitis (FK) with or without perforation. A total of 31 cases of corneal diseases treated by CF surgery between April 2014 and October 2015 were evaluated. Among the 31 cases, 16 cases (male:female, 11:5) with FK were selected. Logistic regression analysis was used to investigate factors associated with complications of CF surgery...
August 2017: Experimental and Therapeutic Medicine
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