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https://www.readbyqxmd.com/read/28224333/anti-neuroinflammatory-effects-of-grossamide-from-hemp-seed-via-suppression-of-tlr-4-mediated-nf-%C3%AE%C2%BAb-signaling-pathways-in-lipopolysaccharide-stimulated-bv2-microglia-cells
#1
Qian Luo, Xiaoli Yan, Larisa Bobrovskaya, Mei Ji, Huiqing Yuan, Hongxiang Lou, Peihong Fan
Grossamide, a representative lignanamide in hemp seed, has been reported to possess potential anti-inflammatory effects. However, the potential anti-neuroinflammatory effects and underlying mechanisms of action of grossamide are still unclear. Therefore, the present study investigated the possible effects and underlying mechanisms of grossamide against lipopolysaccharide (LPS)-induced inflammatory response in BV2 microglia cells. BV2 microglia cells were pre-treated with various concentrations of grossamide before being stimulated with LPS to induce inflammation...
February 21, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28223351/in-vivo-and-in-vitro-proteome-analysis-of-hiv-1-infected-human-cd4-t-cells
#2
Johannes Nemeth, Valentina Vongrad, Karin J Metzner, Victoria Strouvelle, Rainer Weber, Patrick Pedrioli, Ruedi Aebersold, Huldrych Guenthard, Ben C Collins
Host directed therapies against HIV-1 are thought to be critical for long term containment of the HIV-1 pandemic but remain elusive. Since HIV-1 infects and manipulates important effectors of both the innate and adaptive immune system, identifying modulations of the host cell systems in humans during HIV-1 infection may be crucial for the development of immune based therapies. Here, we quantified the changes of the proteome in human CD4+ T cells upon HIV-1 infection, both in vitro and in vivo. A SWATH-MS approach was used to measure the proteome of human primary CD4+ T cells infected with HIV-1 in vitro as well as CD4+ T cells from HIV-1 infected patients with paired samples on and off antiretroviral treatment...
February 21, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28222433/high-glucose-induces-mouse-mesangial-cell-overproliferation-via-inhibition-of-hydrogen-sulfide-synthesis-in-a-tlr-4-dependent-manner
#3
Tao Ding, Wei Chen, Juan Li, Jiarong Ding, Xiaobin Mei, Haiyan Hu
BACKGROUND/AIMS: Overproliferation of mesangial cells was believed to play an important role in the progress of diabetic nephropathy, one of the primary complications of diabetes. Hydrogen sulfide (H2S), a well-known and pungent gas with the distinctive smell of rotten eggs, was discovered to play a protective role in diabetic nephropathy. METHODS: MTT assay was used to examine the viability of mesangial cells. Small interfering RNA was used to knock down the expression of TLR4 while specific inhibitor LY294002 to suppress the function of PI3K...
February 22, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28221243/effects-of-glycyrrhizin-on-the-differentiation-of-myeloid-cells-of-the-heart-and-lungs-in-lipopolysaccharide-induced-septic-mice
#4
Eun-Hye Seo, Ga-Yun Song, Byung Ok Kwak, Chung-Sik Oh, Seung Hyun Lee, Seong-Hyop Kim
BACKGROUND: This study investigated the effects of glycyrrhizin (GR) on the ratio of myeloid-derived suppressor cells (MDSCs) to cluster of differentiation (CD)11b+Gr1 myeloid cells in the heart and lungs in lipopolysaccharide (LPS)-induced septic mice. METHODS: Mice were divided into three groups: Control, LPS, and LPS+GR. After intraperitoneal injection of phosphate-buffered saline for the Control group, LPS for the LPS group, and a combination of LPS and GR for the LPS+GR group, fluorescence-activated cell sorting was utilized to evaluate cytokines and immune cells in the blood, heart, and lungs...
February 17, 2017: Shock
https://www.readbyqxmd.com/read/28215633/tlr-4-mirna-32-5p-fstl1-signaling-regulates-mycobacterial-survival-and-inflammatory-responses-in-mycobacterium-tuberculosis-infected-macrophages
#5
Zhi-Min Zhang, Ai-Rong Zhang, Min Xu, Jun Lou, Wei-Qiang Qiu
Macrophages play a pivotal role in host immune response against mycobacterial infection, which is tightly modulated by multiple factors, including microRNAs. The purpose of the present study was to investigate the biological function and potential mechanism of miR-32-5p in human macrophages during Mycobacterium tuberculosis (M.tb) infection. The results demonstrated that miR-32-5p was robustly enhanced in THP-1 and U937 cells in response to M.tb infection. TLR-4 signaling was required for upregulation of miR-32-5p induced by M...
February 16, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28212352/rta-occupancy-of-the-origin-of-lytic-replication-during-murine-gammaherpesvirus-68-reactivation-from-b-cell-latency
#6
Alexis L Santana, Darby G Oldenburg, Varvara Kirillov, Laraib Malik, Qiwen Dong, Roman Sinayev, Kenneth B Marcu, Douglas W White, Laurie T Krug
RTA, the viral Replication and Transcription Activator, is essential for rhadinovirus lytic gene expression upon de novo infection and reactivation from latency. Lipopolysaccharide (LPS)/toll-like receptor (TLR)4 engagement enhances rhadinovirus reactivation. We developed two new systems to examine the interaction of RTA with host NF-kappaB (NF-κB) signaling during murine gammaherpesvirus 68 (MHV68) infection: a latent B cell line (HE-RIT) inducible for RTA-Flag expression and virus reactivation; and a recombinant virus (MHV68-RTA-Bio) that enabled in vivo biotinylation of RTA in BirA transgenic mice...
February 16, 2017: Pathogens
https://www.readbyqxmd.com/read/28210837/toll-like-receptor-3-tlr-3-tlr-4-and-cd80-expression-in-peripheral-blood-mononuclear-cells-and-urinary-cd80-levels-in-children-with-idiopathic-nephrotic-syndrome
#7
Om P Mishra, Ravindra Kumar, Gopeshwar Narayan, Pradeep Srivastava, Abhishek Abhinay, Rajniti Prasad, Ankur Singh, Vineeta V Batra
BACKGROUND: The aims of this study were (1) to detect toll-like receptor (TLR)-3, TLR-4 and CD80 expression in peripheral blood mononuclear cells (PBMCs) and estimate urinary CD80 levels in children with idiopathic nephrotic syndrome and (2) to investigate the utility of these markers to differentiate between biopsy-proven minimal change disease (MCD) and focal segmental glomeruloscelerosis (FSGS). METHODS: The study included 70 patients with idiopathic nephrotic syndrome (NS), of whom 40 had steroid-sensitive NS (SSNS; 25 with active NS, 15 in remission) and 30 had steroid-resistant NS (SRNS) patients, and 23 healthy controls...
February 16, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28210435/role-of-thr399ile-and-asp299gly-polymorphisms-of-toll-like-receptor-4-gene-in-acute-dental-abscess
#8
Ebrahim Miri-Moghaddam, Narges Farhad-Mollashahi, Elnaz Baghaee, Ali Bazi, Yasaman Garme
BACKGROUND: Apical Periodontitis (AP) is an inflammatory disease that affects the tissues surrounding the root end of a tooth. The disease which is caused by endodontic infections presents in different clinical ways including development of an acute abscess. Recent studies have provided information suggesting role of a multitude of factors in pathogenesis of acute apical abscess (AAA). In this case-control study, our goal was to evaluate the frequency and potential role of two common polymorphisms of toll like receptor-4 (TLR-4) gene; Thr399Ile (1196 C>T) and Asp299Gly (+896 A>G), in 50 patients with AAA as cases and 50 patients with asymptomatic apical periodontitis (AAP) as controls...
February 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28209713/mir-718-represses-pro-inflammatory-cytokine-production-through-targeting-pten
#9
Parisa Kalantari, Omid F Harandi, Sarika Agarwal, Florentina Rus, Evelyn A Kurt-Jones, Katherine A Fitzgerald, Daniel R Caffrey, Douglas T Golenbock
Bacterial sepsis involves a complex interaction between the host immune response and bacterial lipopolysaccharides (LPS). LPS binds Toll-like receptor (TLR) 4, which leads to the release of pro-inflammatory cytokines that are essential for a potent innate immune response against pathogens. The innate immune system is tightly regulated as excessive inflammation can lead to organ failure and death. MicroRNAs have recently emerged as important regulators of the innate immune system. Here, we determined the function of miR-718, which is conserved across mammals and overlaps with the 5 UTR of the Interleukin-1 Receptor-Associated Kinase (IRAK1) gene...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28204830/monoclonal-antibody-against-toll-like-receptor%C3%A2-4-attenuates-ventilator-induced-lung-injury-in-rats-by-inhibiting-myd88-and-nf-%C3%AE%C2%BAb-dependent-signaling
#10
Cuiyuan Huang, Linghui Pan, Fei Lin, Huijun Dai, Ruili Fu
The mechanisms through which mechanical ventilation causes non-infectious inflammatory diseases and lung injury are poorly understood. Animals models of this type of injury suggest that it involves signaling mediated by Toll‑like receptor (TLR)4 and 9. In this study, in order to gain further insight into the involvement of TLR4 in this type of injury, we performed in vivo and in vitro experiments to determine the mechanisms through which TLR4 triggers inflammation. We also examined whether the use of TLR4 monoclonal antibody (mAb) can alleviate this type of injury...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28190471/plant-lectins-conbr-and-cfl-modulate-expression-toll-like-receptors-pro-inflammatory-cytokines-and-reduce-the-bacterial-burden-in-macrophages-infected-with-salmonella-enterica-serovar-typhimurium
#11
Jec Batista, M T Ralph, R V Vaz, Pfc Souza, A B Silva, Dco Nascimento, L T Souza, M V Ramos, P Mastroeni, J V Lima-Filho
BACKGROUND: Plant lectins have long been used in biomedical research as immunomodulators against tumor cells and microbial infections. PURPOSE: To test the ability of plant lectins ConBr (Canavalia brasiliensis) and CFL (Cratylia argentea) to activate antimicrobial and immunomodulatory activities of murine peritoneal macrophages (pMØ) infected with a virulent strain of Salmonella enterica serovar Typhimurium (STm). METHODS: We incubated pMØ with non-toxic amounts of ConBr and CFL either before (preventive schedule) or after (curative schedule) exposure to STm...
February 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28189063/the-nf-%C3%AE%C2%BAb-inhibitor-celastrol-attenuates-acute-hepatic-dysfunction-induced-by-cecal-ligation-and-puncture-in-rats
#12
Ghada S El-Tanbouly, Mohammed S El-Awady, Nermeen A Megahed, Hatem A Salem, Hassan A El-Kashef
Acute hepatic dysfunction associating sepsis is mediated mainly by toll-like receptor-4 (TLR-4)/nuclear factor kappa-B (NF-κB) inflammatory pathway. This study explores potential hepatoprotective effect of the NF-κB inhibitor celastrol in cecal ligation and puncture (CLP) model in rats. Protective effect of celastrol (1mg/kg, i.p., 1h before CLP) was illustrated after 24h by preventing CLP-induced hepatic histopathological changes and elevation in serum hepatic biomarkers [alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and gamma aminotransferase (γ-GT)] without affecting mortality...
February 4, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28182362/upregulation-of-foxo-1-signaling-mediates-the-proinflammatory-cytokine-upregulation-in-the-macrophage-from-polycystic-ovary-syndrome-patients
#13
Ning Li, Xiaoyan Wang, Xiaojie Wang, Hongna Yu, Li Lin, Chengming Sun, Peng Liu, Yongli Chu, Jianqing Hou
BACKGROUND: Chronic activation of macrophage-mediated inflammatory signals in insulin-sensitive metabolic tissues is thought to be one of the causes of insulin resistance-one of the hallmarks of the metabolic syndrome. Insulin resistance is a feature of polycystic ovary syndrome (PCOS) and is related to mitochondrial and endothelial function. METHODS: In the present study, we investigated the phosphorylation level of FoxO 1, which is suppressed by the action of AKT, triggers the TLR4 inflammatory signaling pathway in the macrophages, from polycystic ovary syndrome patients or normal subjects...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28179600/an-ethanol-extract-of-aster-yomena-kitam-honda-inhibits-lipopolysaccharide-induced-inflammatory-responses-in-murine-raw-264-7-macrophages
#14
Hye-Joo Kang, Ji-Suk Jeong, No-Jin Park, Geun-Bae Go, Sung Ok Kim, Cheol Park, Byung Woo Kim, Su-Hyun Hong, Yung Hyun Choi
Aster yomena (Kitam.) Honda has been widely used as a traditional herbal medicine for centuries to treat cough, asthma, insect bites, etc. Recent reports indicate that A. yomena possesses a wide spectrum of pharmacological activities; however, few experiments have described its anti-inflammatory properties. The present study examined the anti-inflammatory effects of an ethanol extract of A. yomena leaves (EEAY) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Treatment with EEAY significantly reduced the secretion of pro-inflammatory molecules, such as nitric oxide and interleukin-1β, in LPS-stimulated RAW 264...
February 7, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28159232/bidirectional-immunomodulating-activity-of-fermented-polysaccharides-from-yupingfeng
#15
Hao Sun, Xueqin Ni, Dong Zeng, Fuqin Zou, Mingyue Yang, Zhirong Peng, Yi Zhou, Yan Zeng, Hui Zhu, Hesong Wang, Zhongqiong Yin, Kangcheng Pan, Bo Jing
Accumulating evidence has proven the immunomodulating activity of Yupingfeng. This study compared the immunomodulatory activity in vitro of the unfermented Yupingfeng dreg polysaccharides (UYDP) with that of the fermented Yupingfeng dreg polysaccharides (FYDP) obtained using Rhizopus oligosporus SH. Results consistently elucidated the duality of the immunomodulatory roles of UYDP and FYDP in regulating proliferation, and cytokines expressions in murine lymphocytes and macrophages. Compared with UYDP, FYDP effectively enhanced the proliferation of lymphocytes and promoted mRNA expression of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, INF-γ, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and TLR-4 in lymphocytes and macrophages...
February 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28152042/effect-of-pregabalin-administration-upon-reperfusion-in-a-rat-model-of-hyperglycemic-stroke-mechanistic-insights-associated-with-high-mobility-group-box-1
#16
Young Song, Ji-Hae Jun, Eun-Jung Shin, Young-Lan Kwak, Jeon-Soo Shin, Jae-Kwang Shim
Hyperglycemia, which reduces the efficacy of treatments and worsens clinical outcomes, is common in stroke. Ability of pregabalin to reduce neuroexcitotoxicity may provide protection against stroke, even under hyperglycemia. We investigated its protective effect against hyperglycemic stroke and its possible molecular mechanisms. Male Wistar rats administered dextrose to cause hyperglycemia, underwent middle cerebral artery occlusion for 1 h and subsequent reperfusion. Rats were treated with an intraperitoneal injection of 30 mg/kg pregabalin or an equal amount of normal saline at the onset of reperfusion (n = 16 per group)...
2017: PloS One
https://www.readbyqxmd.com/read/28131803/myd88-gene-knockout-attenuates-paraquat-induced-acute-lung-injury
#17
Haitao Shen, Na Wu, Yu Wang, Feng Guo, Lianghong Chen, Zhigang Zhang, Dong Jia, Min Zhao
OBJECTIVE: This study investigated the role of myeloid differentiation factor 88 (MyD88) in paraquat-induced acute lung injury (ALI). METHODS: C57BL mice were divided into the control group, paraquat group, MyD88 knockout (KO) group, and MyD88 KO plus paraquat group. At 48h after paraquat poisoning, serum and lung tissues were collected. ELISA was employed to detect tumor necrosis factor-α (TNF-α) and interleukine-1β (IL-1β) contents in serum. Lung tissues were processed for hematoxylin-eosin staining, followed by histological scoring...
January 25, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28130575/modulation-of-cyclophosphamide-induced-cardiotoxicity-by-methyl-palmitate
#18
Dina S El-Agamy, Mohamed A Elkablawy, Hany M Abo-Haded
PURPOSE: Cyclophosphamide (CP) is a frequently used anticancer and immunosuppressant although its use has been associated with severe cardiotoxicity. The present study examined the ability of methyl palmitate (MP) to counteract CP-induced cardiotoxicity. METHODS: Adult male Wistar rats were divided into four groups. The first one served as control while the second received a single injection of CP (200 mg/kg, i.p.). The other two groups were administered MP at two different dose levels (300, 400 mg/kg) for 10 days before and 7 days after CP single injection...
February 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28128358/galectin-3-released-in-response-to-traumatic-brain-injury-acts-as-an-alarmin-orchestrating-brain-immune-response-and-promoting-neurodegeneration
#19
Ping Kei Yip, Alejandro Carrillo-Jimenez, Paul King, Anna Vilalta, Koji Nomura, Chi Cheng Chau, Alexander Michael Scott Egerton, Zhuo-Hao Liu, Ashray Jayaram Shetty, Jordi L Tremoleda, Meirion Davies, Tomas Deierborg, John V Priestley, Guy Charles Brown, Adina Teodora Michael-Titus, Jose Luis Venero, Miguel Angel Burguillos
Traumatic brain injury (TBI) is currently a major cause of morbidity and poor quality of life in Western society, with an estimate of 2.5 million people affected per year in Europe, indicating the need for advances in TBI treatment. Within the first 24 h after TBI, several inflammatory response factors become upregulated, including the lectin galectin-3. In this study, using a controlled cortical impact (CCI) model of head injury, we show a large increase in the expression of galectin-3 in microglia and also an increase in the released form of galectin-3 in the cerebrospinal fluid (CSF) 24 h after head injury...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28122919/soluble-cd14-acts-as-a-damp-in-human-macrophages-origin-and-involvement-in-inflammatory-cytokine-chemokine-production
#20
Manuella Lévêque, Karin Simonin-Le Jeune, Stéphane Jouneau, Solenn Moulis, Benoit Desrues, Chantal Belleguic, Graziella Brinchault, Sophie Le Trionnaire, Jean-Pierre Gangneux, Marie-Thérèse Dimanche-Boitrel, Corinne Martin-Chouly
The innate immune system is able to detect bacterial LPS through the pattern recognition receptor CD14, which delivers LPS to various TLR signaling complexes that subsequently induce intracellular proinflammatory signaling cascades. In a previous study, we showed the overproduction of the soluble form of CD14 (sCD14) by macrophages from patients with cystic fibrosis (CF). CF is an autosomal recessive disorder that is caused by mutations in the gene that encodes the CFTR protein and is characterized by persistent inflammation...
January 25, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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