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cucurbita moschata

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https://www.readbyqxmd.com/read/29320569/transcriptome-profiling-of-pumpkin-cucurbita-moschata-duch-leaves-infected-with-powdery-mildew
#1
Wei-Li Guo, Bi-Hua Chen, Xue-Jin Chen, Yan-Yan Guo, He-Lian Yang, Xin-Zheng Li, Guang-Yin Wang
Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using RNA sequencing (RNA-Seq). The inbred line "112-2" has been purified over 8 consecutive generations of self-pollination and shows high resistance to PM...
2018: PloS One
https://www.readbyqxmd.com/read/29234347/ectopic-expression-of-pumpkin-nac-transcription-factor-cmnac1-improves-multiple-abiotic-stress-tolerance-in-arabidopsis
#2
Haishun Cao, Li Wang, Muhammad A Nawaz, Mengliang Niu, Jingyu Sun, Junjun Xie, Qiusheng Kong, Yuan Huang, Fei Cheng, Zhilong Bie
Drought, cold and salinity are the major environmental stresses that limit agricultural productivity. NAC transcription factors regulate the stress response in plants. Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related NAC genes in this crop are largely unknown. This study reports the function of CmNAC1, a stress-responsive pumpkin NAC domain protein. The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29165479/evaluation-of-the-impact-of-food-matrix-change-on-the-in-vitro-bioaccessibility-of-carotenoids-in-pumpkin-cucurbita-moschata-slices-during-two-drying-processes
#3
Zhongyuan Zhang, Xiaoyan Wang, Yixiang Li, Qiuyu Wei, Chunju Liu, Meimei Nie, Dajing Li, Yadong Xiao, Chunquan Liu, Lang Xu, Min Zhang, Ning Jiang
The food matrix is a limiting factor in determining the bioaccessibility of carotenoids. The impact of food matrix change on the bioaccessibility of carotenoids during drying processes is still unknown. The effect of intermittent microwave vacuum-assisted drying (IMVD) and hot air drying (HAD) on the in vitro liberation and micellization of carotenoids in pumpkin slices was studied. This variable depended on the changes of the matrix driven by the drying process. Different changes in the cell morphology and carotenoid distribution of pumpkin slices during the two processing methods were observed...
November 22, 2017: Food & Function
https://www.readbyqxmd.com/read/29112324/de-novo-assembly-of-the-zucchini-genome-reveals-a-whole-genome-duplication-associated-with-the-origin-of-the-cucurbita-genus
#4
Javier Montero-Pau, José Blanca, Aureliano Bombarely, Peio Ziarsolo, Cristina Esteras, Carlos Martí-Gómez, María Ferriol, Pedro Gómez, Manuel Jamilena, Lukas Mueller, Belén Picó, Joaquín Cañizares
The Cucurbita genus (squashes, pumpkins and gourds) includes important domesticated species such as C. pepo, C. maxima and C. moschata. In this study, we present a high-quality draft of the zucchini (C. pepo) genome. The assembly has a size of 263 Mb, a scaffold N50 of 1.8 Mb, and 34,240 gene models. It includes 92% of the conserved BUSCO core gene set, and it is estimated to cover 93.0% of the genome. The genome is organized in 20 pseudomolecules that represent 81.4% of the assembly, and it is integrated with a genetic map of 7,718 SNPs...
November 7, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29102765/can-highly-cited-herbs-in-ancient-traditional-chinese-medicine-formulas-and-modern-publications-predict-therapeutic-targets-for-diabetes-mellitus
#5
Huijun Wang, Songshan Shi, Shunchun Wang
ETHNOPHARMACOLOGICAL RELEVANCE: The prevalence of diabetes among all age groups worldwide was estimated to be more than 382 million in 2013. Traditional Chinese medicine (TCM) has been practiced for thousands of years, and substantial valuable experience and prescriptions have been accumulated in the TCM system for the treatment of diabetes. In recent decades, a large amount of experimental and clinical data has been published on the use of herbal medicines related to these ancient TCM prescriptions...
November 2, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29093727/identification-and-genetic-characterization-of-ralstonia-solanacearum-species-complex-isolates-from-cucurbita-maxima-in-china
#6
Xiaoman She, Lin Yu, Guobing Lan, Yafei Tang, Zifu He
Ralstonia solanacearum species complex is a devastating phytopathogen with an unusually wide host range, and new host plants are continuously being discovered. In June 2016, a new bacterial wilt on Cucurbita maxima was observed in Guangdong province, China. Initially, in the adult plant stage, several leaves of each plant withered suddenly and drooped; the plant then wilted completely, and the color of their vasculature changed to dark brown, ultimately causing the entire plant to die. Creamy-whitish bacterial masses were observed to ooze from crosscut stems of these diseased plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28986571/a-high-density-linkage-map-and-qtl-mapping-of-fruit-related-traits-in-pumpkin-cucurbita-moschata-duch
#7
Yu-Juan Zhong, Yang-Yang Zhou, Jun-Xing Li, Ting Yu, Ting-Quan Wu, Jian-Ning Luo, Shao-Bo Luo, He-Xun Huang
Pumpkin (Cucurbita moschata) is an economically worldwide crop. Few quantitative trait loci (QTLs) were reported previously due to the lack of genomic and genetic resources. In this study, a high-density linkage map of C. moschata was structured by double-digest restriction site-associated DNA sequencing, using 200 F2 individuals of CMO-1 × CMO-97. By filtering 74,899 SNPs, a total of 3,470 high quality SNP markers were assigned to the map spanning a total genetic distance of 3087.03 cM on 20 linkage groups (LGs) with an average genetic distance of 0...
October 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28974797/evaluation-of-three-pumpkin-species-correlation-with-physicochemical-antioxidant-properties-and-classification-using-spme-gc-ms-and-e-nose-methods
#8
Chun-Li Zhou, Li Mi, Xue-Yan Hu, Bi-Hua Zhu
To ascertain the most discriminant variables for three pumpkin species principal component analysis (PCA) was performed. Twenty-four parameters (pH, conductivity, sucrose, glucose, total soluble solids, L*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, PPO activity, POD activity, total flavonoids, vitamin E, total phenolics, DPPH, FRAP, β-carotene, and aroma) were considered. The studied pumpkin species were Cucurbita maxima, Cucurbita moschata, and Cucurbita pepo...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28917590/karyotype-stability-and-unbiased-fractionation-in-the-paleo-allotetraploid-cucurbita-genomes
#9
Honghe Sun, Shan Wu, Guoyu Zhang, Chen Jiao, Shaogui Guo, Yi Ren, Jie Zhang, Haiying Zhang, Guoyi Gong, Zhangcai Jia, Fan Zhang, Jiaxing Tian, William J Lucas, Jeff J Doyle, Haizhen Li, Zhangjun Fei, Yong Xu
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago (Mya), respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C...
October 9, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28861228/resurgence-of-cucurbit-downy-mildew-in-the-united-states-insights-from-comparative-genomic-analysis-of-pseudoperonospora-cubensis
#10
Anna Thomas, Ignazio Carbone, Kisurb Choe, Lina M Quesada-Ocampo, Peter S Ojiambo
Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew (CDM), is known to exhibit host specialization. The virulence of different isolates of the pathogen can be classified into pathotypes based on their compatibility with a differential set composed of specific cucurbit host types. However, the genetic basis of host specialization within P. cubensis is not yet known. Total genomic DNA extracted from nine isolates of P. cubensis collected from 2008 to 2013 from diverse cucurbit host types (Cucumis sativus, C...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28852445/evaluation-of-phytochemicals-antioxidant-and-burn-wound-healing-activities-of-cucurbita-moschata-duchesne-fruit-peel
#11
Roodabeh Bahramsoltani, Mohammad Hosein Farzaei, Amir Hossein Abdolghaffari, Roja Rahimi, Nasrin Samadi, Mohammad Heidari, Mohammadamin Esfandyari, Maryam Baeeri, Gholamreza Hassanzadeh, Mohammad Abdollahi, Saba Soltani, Ali Pourvaziri, Gholamreza Amin
OBJECTIVES: Cucurbita moschata Duchesne (pumpkin) is a well-known plant with several pharmacological effects. The aim of the present study was to assess burn wound healing activity of C. moschata peel extract (CE). Also, standardized CE was assessed for antioxidant activity and antibacterial effects against major pathogens of burns. MATERIALS AND METHODS: Healing properties of topical preparation of 10% and 20% concentrations of CE were assessed on second degree burn in rats during a 14-day period as well as histological studies, total antioxidant power, lipid peroxidation and total thiol content of skin tissue samples...
July 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28586926/cytoprotective-effects-of-pumpkin-cucurbita-moschata-fruit-extract-against-oxidative-stress-and-carbonyl-stress
#12
Reyhaneh Shayesteh, Mohammad Kamalinejad, Hasan Adiban, Azin Kardan, Fariborz Keyhanfar, Mohammad Reza Eskandari
Background Diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. Diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. In the meantime, reactive oxygen species (ROS) and reactive carbonyl species (RCS) have key mediatory roles in the development and progression of diabetes complications...
October 2017: Drug Research
https://www.readbyqxmd.com/read/28551134/a-green-analytical-method-for-benzoyl-peroxide-determination-by-a-sequential-injection-spectrophotometry-using-natural-reagent-extracts-from-pumpkin
#13
Sam-Ang Supharoek, Kraingkrai Ponhong, Kate Grudpan
A green and environmental friendly method with simple sequential injection spectrophotometry using natural reagent extract from pumpkin (Cucurbita moschata Decne) for determination of benzoyl peroxide (BPO) was developed. The maximum absorption of the yellow crude extract containing the β-carotene compound occurred at a wavelength of 450nm. The yellow extract solution was bleached by BPO to change the color to a colorless solution. The optimum conditions of the proposed method, such as extraction solvent influence of pH, dilution of pumpkin extracts solution, aspiration volume of reagent and sample, flow reversal, incubation time, mixing coil length, and dispensing flow rate were studied and optimized...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28369915/fruit-quality-of-seedless-watermelon-grafted-onto-squash-rootstocks-under-different-production-systems
#14
Qianru Liu, Xin Zhao, Jeffrey K Brecht, Charles A Sims, Tatiana Sanchez, Nicholas S Dufault
BACKGROUND: The market demand for seedless watermelon has been continuously increasing because of consumer preference. Grafting is a useful tool to manage soilborne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. Currently, most research has focused on seeded cultivars, while grafting effects on seedless watermelons remain largely unknown. This multi-season study was conducted to assess the effects of squash rootstocks, including both Cucurbita maxima × C...
March 29, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28242253/dechlorination-and-chlorine-rearrangement-of-1-2-5-5-6-9-10-heptachlorodecane-mediated-by-the-whole-pumpkin-seedlings
#15
Yanlin Li, Xingwang Hou, Miao Yu, Qunfang Zhou, Jiyan Liu, Jerald L Schnoor, Guibin Jiang
Short chain chlorinated paraffins (SCCPs) are ubiquitously present as persistent organic pollutants in the environment. However, little information on the interaction of SCCPs with plants is currently available. In this work, young pumpkin plants (Cucurbita maxima × C. Moschata) were hydroponically exposed to the congener of chlorinated decane, 1,2,5,5,6,9,10-heptachlorodecane (1,2,5,5,6,9,10-HepCD), to investigate the uptake, translocation and transformation of chlorinated decanes in the intact plants...
May 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27936008/cultivar-based-introgression-mapping-reveals-wild-species-derived-pm-0-the-major-powdery-mildew-resistance-locus-in-squash
#16
William L Holdsworth, Kyle E LaPlant, Duane C Bell, Molly M Jahn, Michael Mazourek
Powdery mildew is a major fungal disease on squash and pumpkin (Cucurbita spp.) in the US and throughout the world. Genetic resistance to the disease is not known to occur naturally within Cucurbita pepo and only infrequently in Cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species Cucurbita okeechobeensis subsp. martinezii. At present, this gene, Pm-0, is used extensively in breeding, and is found in nearly all powdery mildew-resistant C...
2016: PloS One
https://www.readbyqxmd.com/read/27935073/no-accumulation-alleviates-h2-o2-dependent-oxidative-damage-induced-by-ca-no3-2-stress-in-the-leaves-of-pumpkin-grafted-cucumber-seedlings
#17
Lin Li, Sheng Shu, Qing Xu, Ya-Hong An, Jin Sun, Shi-Rong Guo
Nitric oxide (NO) and hydrogen peroxide (H2 O2 ), two important signaling molecules, are stimulated in plants by abiotic stresses. In this study, we investigated the role of NO and its interplay with H2 O2 in the response of self-grafted (S-G) and salt-tolerant pumpkin-grafted (Cucurbita maxima × C. moschata) cucumber seedlings to 80 mM Ca(NO3 )2 stress. Endogenous NO and H2 O2 production in S-G seedlings increased in a time-dependent manner, reaching maximum levels after 24 h of Ca(NO3 )2 stress. In contrast, a transient increase in NO production, accompanied by H2 O2 accumulation, was observed at 2 h in rootstock-grafted plants...
May 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/27701737/isolation-and-functional-characterization-of-cslsi1-a-silicon-transporter-gene-in-cucumis-sativus
#18
Hao Sun, Jia Guo, Yaoke Duan, Tiantian Zhang, Heqiang Huo, Haijun Gong
Cucumber (Cucumis sativus) is a widely grown cucurbitaceous vegetable that exhibits a relatively high capacity for silicon (Si) accumulation, but the molecular mechanism for silicon uptake remains to be clarified. Here we isolated and characterized CsLsi1, a gene encoding a silicon transporter in cucumber (cv. Mch-4). CsLsi1 shares 55.70 and 90.63% homology with the Lsi1s of a monocot and dicot, rice (Oryza sativa) and pumpkin (Cucurbita moschata), respectively. CsLsi1 was predominantly expressed in the roots, and application of exogenous silicon suppressed its expression...
February 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/27697378/carboxylesterase-involved-metabolism-of-di-n-butyl-phthalate-in-pumpkin-cucurbita-moschata-seedlings
#19
Qingqi Lin, Siyuan Chen, Yuanqing Chao, Xiongfei Huang, Shizhong Wang, Rongliang Qiu
Uptake and accumulation by plants is a significant pathway in the migration and transformation of phthalate esters (PAEs) in the environment. However, limited information is available on the mechanisms of PAE metabolism in plants. Here, we investigated the metabolism of di-n-butyl phthalate (DnBP), one of the most frequently detected PAEs, in pumpkin (Cucurbita moschata) seedlings via a series of hydroponic experiments with an initial concentration of 10 mg L(-1). DnBP hydrolysis occurred primarily in the root, and two of its metabolites, mono-n-butyl phthalate (MnBP) and phthalic acid (PA), were detected in all plant tissues...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27621740/can-adverse-effects-of-acidity-and-aluminum-toxicity-be-alleviated-by-appropriate-rootstock-selection-in-cucumber
#20
Youssef Rouphael, Elvira Rea, Mariateresa Cardarelli, Michael Bitterlich, Dietmar Schwarz, Giuseppe Colla
Low-pH and aluminum (Al) stresses are the major constraints that limit crop yield in acidic soils. Grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and Al toxicity. Two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (Cucumis sativus L. "Ekron") either non-grafted or grafted onto "P360" (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne; E/C) or figleaf gourd (Cucurbita ficifolia Bouché; E/F)...
2016: Frontiers in Plant Science
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