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https://www.readbyqxmd.com/read/29783623/optimization-of-ultrasound-assisted-extraction-and-structural-characterization-of-the-polysaccharide-from-pumpkin-cucurbita-moschata-seeds
#1
Libo Wang, Long Cheng, Fangcheng Liu, Tengfei Li, Zeyuan Yu, Yaqin Xu, Yu Yang
In the present study, ultrasound-assisted extraction (UAE) of crude polysaccharides (PSP) from pumpkin seeds was optimized by response surface method (RSM). The polysaccharide yield (2.29 ± 0.14%), which agreed closely with the theoretical predicted value 2.40%, was obtained under the optimal extraction conditions: extraction time 24 min, extraction temperature 50 °C, ultrasonic power 347 W, and liquid to solid ratio 23 mL/g. After further purification by two-step column chromatography, a novel polysaccharide (PSP-1) was isolated from pumpkin seeds...
May 18, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29729569/enhanced-organic-contaminants-accumulation-in-crops-mechanisms-interactions-with-engineered-nanomaterials-in-soil
#2
Xiang Wu, Wei Wang, Lizhong Zhu
The mechanism of enhanced accumulation of organic contaminants in crops with engineered nanomaterials (ENMs) were investigated by co-exposure of crops (Ipomoea aquatica Forsk (Swamp morning-glory), Cucumis sativus L. (cucumber), Zea mays L. (corn), Spinacia oleracea L. (spinach) and Cucurbita moschata (pumpkin))to a range of chemicals (polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and polybrominated diphenyl ether (PBDE)) and ENMs (TiO2 , Ag, Al2 O3 , graphene, carbon nanotubes (CNTs)) in soil...
May 2, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29725353/enhancement-of-lipid-metabolism-and-hepatic-stability-in-fat-induced-obese-mice-by-fermented-cucurbita-moschata-extract
#3
Md Akil Hossain, Seung-Jin Lee, Na-Hye Park, Biruk Tesfaye Birhanu, Abraham Fikru Mechesso, Ji-Yong Park, Eun-Jin Park, Sam-Pin Lee, Sun-Joo Youn, Seung-Chun Park
The aim of this study was to evaluate the potentials of fermented Cucurbita moschata extract (FCME) in the treatment of obesity and nonalcoholic fatty liver disease (NAFLD). Five-week-old male C57BL/6 mice were assigned to 6 groups and treated for 8 weeks by feeding the normal diet (ND) and high fat diet (HFD) with and without FCME. Changes in body weight gain and consumption of feed and water were recorded. Major organs, adipose tissues, and blood samples were collected after the experimental period. The serum lipid profile, histological features of liver and adipose tissues, and mRNA expression of different adipogenic/lipogenic genes from liver tissue were evaluated...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29718492/ovipositional-preferences-of-two-squash-bug-species-anasa-tristis-and-anasa-armigera-heteroptera-coreidae-for-different-cultivars-and-species-of-cucurbitaceae
#4
Mary L Cornelius
The ovipositional preferences of two squash bug species, Anasa tristis (DeGeer) (Heteroptera: Coreidae) and Anasa armigera Say (Heteroptera: Coreidae), were evaluated in paired choice tests of different species and cultivars of plants in the family Cucurbitaceae. Females of A. tristis preferred to oviposit on the cultivar from which they were reared over three other cultivars of the same species. However, females did not show any ovipositional preference for different cultivars when they had no previous exposure to either cultivar...
March 1, 2018: Journal of Insect Science
https://www.readbyqxmd.com/read/29629242/pumpkin-powdery-mildew-disease-severity-influences-the-fungal-diversity-of-the-phyllosphere
#5
Zhuo Zhang, Luyun Luo, Xinqiu Tan, Xiao Kong, Jianguo Yang, Duanhua Wang, Deyong Zhang, Decai Jin, Yong Liu
Phyllosphere microbiota play a crucial role in plant-environment interactions and their microbial community and function are influenced by biotic and abiotic factors. However, there is little research on how pathogens affect the microbial community of phyllosphere fungi. In this study, we collected 16 pumpkin ( Cucurbita moschata ) leaf samples which exhibited powdery mildew disease, with a severity ranging from L1 (least severe) to L4 (most severe). The fungal community structure and diversity was examined by Illumina MiSeq sequencing of the internal transcribed spacer (ITS) region of ribosomal RNA genes...
2018: PeerJ
https://www.readbyqxmd.com/read/29320569/transcriptome-profiling-of-pumpkin-cucurbita-moschata-duch-leaves-infected-with-powdery-mildew
#6
Wei-Li Guo, Bi-Hua Chen, Xue-Jin Chen, Yan-Yan Guo, He-Lian Yang, Xin-Zheng Li, Guang-Yin Wang
Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using RNA sequencing (RNA-Seq). The inbred line "112-2" has been purified over 8 consecutive generations of self-pollination and shows high resistance to PM...
2018: PloS One
https://www.readbyqxmd.com/read/29234347/ectopic-expression-of-pumpkin-nac-transcription-factor-cmnac1-improves-multiple-abiotic-stress-tolerance-in-arabidopsis
#7
Haishun Cao, Li Wang, Muhammad A Nawaz, Mengliang Niu, Jingyu Sun, Junjun Xie, Qiusheng Kong, Yuan Huang, Fei Cheng, Zhilong Bie
Drought, cold and salinity are the major environmental stresses that limit agricultural productivity. NAC transcription factors regulate the stress response in plants. Pumpkin ( Cucurbita moschata ) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related NAC genes in this crop are largely unknown. This study reports the function of CmNAC1, a stress-responsive pumpkin NAC domain protein. The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29165479/evaluation-of-the-impact-of-food-matrix-change-on-the-in-vitro-bioaccessibility-of-carotenoids-in-pumpkin-cucurbita-moschata-slices-during-two-drying-processes
#8
Zhongyuan Zhang, Xiaoyan Wang, Yixiang Li, Qiuyu Wei, Chunju Liu, Meimei Nie, Dajing Li, Yadong Xiao, Chunquan Liu, Lang Xu, Min Zhang, Ning Jiang
The food matrix is a limiting factor in determining the bioaccessibility of carotenoids. The impact of food matrix change on the bioaccessibility of carotenoids during drying processes is still unknown. The effect of intermittent microwave vacuum-assisted drying (IMVD) and hot air drying (HAD) on the in vitro liberation and micellization of carotenoids in pumpkin slices was studied. This variable depended on the changes of the matrix driven by the drying process. Different changes in the cell morphology and carotenoid distribution of pumpkin slices during the two processing methods were observed...
December 13, 2017: Food & Function
https://www.readbyqxmd.com/read/29112324/de-novo-assembly-of-the-zucchini-genome-reveals-a-whole-genome-duplication-associated-with-the-origin-of-the-cucurbita-genus
#9
Javier Montero-Pau, José Blanca, Aureliano Bombarely, Peio Ziarsolo, Cristina Esteras, Carlos Martí-Gómez, María Ferriol, Pedro Gómez, Manuel Jamilena, Lukas Mueller, Belén Picó, Joaquín Cañizares
The Cucurbita genus (squashes, pumpkins and gourds) includes important domesticated species such as C. pepo, C. maxima and C. moschata. In this study, we present a high-quality draft of the zucchini (C. pepo) genome. The assembly has a size of 263 Mb, a scaffold N50 of 1.8 Mb and 34 240 gene models. It includes 92% of the conserved BUSCO core gene set, and it is estimated to cover 93.0% of the genome. The genome is organized in 20 pseudomolecules that represent 81.4% of the assembly, and it is integrated with a genetic map of 7718 SNPs...
November 7, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29102765/can-highly-cited-herbs-in-ancient-traditional-chinese-medicine-formulas-and-modern-publications-predict-therapeutic-targets-for-diabetes-mellitus
#10
Huijun Wang, Songshan Shi, Shunchun Wang
ETHNOPHARMACOLOGICAL RELEVANCE: The prevalence of diabetes among all age groups worldwide was estimated to be more than 382 million in 2013. Traditional Chinese medicine (TCM) has been practiced for thousands of years, and substantial valuable experience and prescriptions have been accumulated in the TCM system for the treatment of diabetes. In recent decades, a large amount of experimental and clinical data has been published on the use of herbal medicines related to these ancient TCM prescriptions...
March 1, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29093727/identification-and-genetic-characterization-of-ralstonia-solanacearum-species-complex-isolates-from-cucurbita-maxima-in-china
#11
Xiaoman She, Lin Yu, Guobing Lan, Yafei Tang, Zifu He
Ralstonia solanacearum species complex is a devastating phytopathogen with an unusually wide host range, and new host plants are continuously being discovered. In June 2016, a new bacterial wilt on Cucurbita maxima was observed in Guangdong province, China. Initially, in the adult plant stage, several leaves of each plant withered suddenly and drooped; the plant then wilted completely, and the color of their vasculature changed to dark brown, ultimately causing the entire plant to die. Creamy-whitish bacterial masses were observed to ooze from crosscut stems of these diseased plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28986571/a-high-density-linkage-map-and-qtl-mapping-of-fruit-related-traits-in-pumpkin-cucurbita-moschata-duch
#12
Yu-Juan Zhong, Yang-Yang Zhou, Jun-Xing Li, Ting Yu, Ting-Quan Wu, Jian-Ning Luo, Shao-Bo Luo, He-Xun Huang
Pumpkin (Cucurbita moschata) is an economically worldwide crop. Few quantitative trait loci (QTLs) were reported previously due to the lack of genomic and genetic resources. In this study, a high-density linkage map of C. moschata was structured by double-digest restriction site-associated DNA sequencing, using 200 F2 individuals of CMO-1 × CMO-97. By filtering 74,899 SNPs, a total of 3,470 high quality SNP markers were assigned to the map spanning a total genetic distance of 3087.03 cM on 20 linkage groups (LGs) with an average genetic distance of 0...
October 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28974797/evaluation-of-three-pumpkin-species-correlation-with-physicochemical-antioxidant-properties-and-classification-using-spme-gc-ms-and-e-nose-methods
#13
Chun-Li Zhou, Li Mi, Xue-Yan Hu, Bi-Hua Zhu
To ascertain the most discriminant variables for three pumpkin species principal component analysis (PCA) was performed. Twenty-four parameters (pH, conductivity, sucrose, glucose, total soluble solids, L*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, PPO activity, POD activity, total flavonoids, vitamin E, total phenolics, DPPH, FRAP, β-carotene, and aroma) were considered. The studied pumpkin species were Cucurbita maxima, Cucurbita moschata, and Cucurbita pepo...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28917590/karyotype-stability-and-unbiased-fractionation-in-the-paleo-allotetraploid-cucurbita-genomes
#14
Honghe Sun, Shan Wu, Guoyu Zhang, Chen Jiao, Shaogui Guo, Yi Ren, Jie Zhang, Haiying Zhang, Guoyi Gong, Zhangcai Jia, Fan Zhang, Jiaxing Tian, William J Lucas, Jeff J Doyle, Haizhen Li, Zhangjun Fei, Yong Xu
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago (Mya), respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C...
October 9, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28861228/resurgence-of-cucurbit-downy-mildew-in-the-united-states-insights-from-comparative-genomic-analysis-of-pseudoperonospora-cubensis
#15
Anna Thomas, Ignazio Carbone, Kisurb Choe, Lina M Quesada-Ocampo, Peter S Ojiambo
Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew (CDM), is known to exhibit host specialization. The virulence of different isolates of the pathogen can be classified into pathotypes based on their compatibility with a differential set composed of specific cucurbit host types. However, the genetic basis of host specialization within P. cubensis is not yet known. Total genomic DNA extracted from nine isolates of P. cubensis collected from 2008 to 2013 from diverse cucurbit host types (Cucumis sativus, C...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28852445/evaluation-of-phytochemicals-antioxidant-and-burn-wound-healing-activities-of-cucurbita-moschata-duchesne-fruit-peel
#16
Roodabeh Bahramsoltani, Mohammad Hosein Farzaei, Amir Hossein Abdolghaffari, Roja Rahimi, Nasrin Samadi, Mohammad Heidari, Mohammadamin Esfandyari, Maryam Baeeri, Gholamreza Hassanzadeh, Mohammad Abdollahi, Saba Soltani, Ali Pourvaziri, Gholamreza Amin
OBJECTIVES: Cucurbita moschata Duchesne (pumpkin) is a well-known plant with several pharmacological effects. The aim of the present study was to assess burn wound healing activity of C. moschata peel extract (CE). Also, standardized CE was assessed for antioxidant activity and antibacterial effects against major pathogens of burns. MATERIALS AND METHODS: Healing properties of topical preparation of 10% and 20% concentrations of CE were assessed on second degree burn in rats during a 14-day period as well as histological studies, total antioxidant power, lipid peroxidation and total thiol content of skin tissue samples...
July 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28586926/cytoprotective-effects-of-pumpkin-cucurbita-moschata-fruit-extract-against-oxidative-stress-and-carbonyl-stress
#17
Reyhaneh Shayesteh, Mohammad Kamalinejad, Hasan Adiban, Azin Kardan, Fariborz Keyhanfar, Mohammad Reza Eskandari
Background Diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. Diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. In the meantime, reactive oxygen species (ROS) and reactive carbonyl species (RCS) have key mediatory roles in the development and progression of diabetes complications...
October 2017: Drug Research
https://www.readbyqxmd.com/read/28551134/a-green-analytical-method-for-benzoyl-peroxide-determination-by-a-sequential-injection-spectrophotometry-using-natural-reagent-extracts-from-pumpkin
#18
Sam-Ang Supharoek, Kraingkrai Ponhong, Kate Grudpan
A green and environmental friendly method with simple sequential injection spectrophotometry using natural reagent extract from pumpkin (Cucurbita moschata Decne) for determination of benzoyl peroxide (BPO) was developed. The maximum absorption of the yellow crude extract containing the β-carotene compound occurred at a wavelength of 450nm. The yellow extract solution was bleached by BPO to change the color to a colorless solution. The optimum conditions of the proposed method, such as extraction solvent influence of pH, dilution of pumpkin extracts solution, aspiration volume of reagent and sample, flow reversal, incubation time, mixing coil length, and dispensing flow rate were studied and optimized...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28369915/fruit-quality-of-seedless-watermelon-grafted-onto-squash-rootstocks-under-different-production-systems
#19
Qianru Liu, Xin Zhao, Jeffrey K Brecht, Charles A Sims, Tatiana Sanchez, Nicholas S Dufault
BACKGROUND: The market demand for seedless watermelon has been continuously increasing because of consumer preference. Grafting is a useful tool to manage soilborne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. Currently, most research has focused on seeded cultivars, while grafting effects on seedless watermelons remain largely unknown. This multi-season study was conducted to assess the effects of squash rootstocks, including both Cucurbita maxima × C...
November 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28242253/dechlorination-and-chlorine-rearrangement-of-1-2-5-5-6-9-10-heptachlorodecane-mediated-by-the-whole-pumpkin-seedlings
#20
Yanlin Li, Xingwang Hou, Miao Yu, Qunfang Zhou, Jiyan Liu, Jerald L Schnoor, Guibin Jiang
Short chain chlorinated paraffins (SCCPs) are ubiquitously present as persistent organic pollutants in the environment. However, little information on the interaction of SCCPs with plants is currently available. In this work, young pumpkin plants (Cucurbita maxima × C. Moschata) were hydroponically exposed to the congener of chlorinated decane, 1,2,5,5,6,9,10-heptachlorodecane (1,2,5,5,6,9,10-HepCD), to investigate the uptake, translocation and transformation of chlorinated decanes in the intact plants...
May 2017: Environmental Pollution
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