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https://www.readbyqxmd.com/read/28917088/depression-anxiety-ptsd-and-comorbidity-in-perinatal-women-in-turkey-a-longitudinal-population-based-study
#1
Pelin Dikmen-Yildiz, Susan Ayers, Louise Phillips
OBJECTIVE: (a) to assess prevalence of depression, anxiety and post-traumatic stress disorder (PTSD) and their comorbidity among women during the perinatal period (b) to examine course of those disorders from pregnancy to 6 months postpartum (c) to determine the rates of new-onset cases at 4-6 weeks and 6 months postpartum. DESIGN: A longitudinal population-based study in which participants completed psychosocial measures of depression, anxiety and PTSD in pregnancy (n = 950), 4-6 weeks (n = 858) and 6 months (n = 829) after birth...
September 6, 2017: Midwifery
https://www.readbyqxmd.com/read/28913702/impaired-fertility-and-motor-function-in-a-zebrafish-model-for-classic-galactosemia
#2
Jo M Vanoevelen, M Estela Rubio-Gozalbo, Britt van Erven, Jörgen Bierau, Xiaoping Huang, Gerard T Berry, Rein Vos, Ana I Coelho
Classic galactosemia is a genetic disorder of galactose metabolism, caused by severe deficiency of galactose-1-phosphate uridylyltransferase (GALT) enzyme activity due to mutations of the GALT gene. Its pathogenesis is still not fully elucidated, and a therapy that prevents chronic impairments is lacking. In order to move research forward, there is a high need for a novel animal model, which allows organ studies throughout development and high-throughput screening of pharmacologic compounds. Here, we describe the generation of a galt knockout zebrafish model and present its phenotypical characterization...
September 14, 2017: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/28903736/factors-associated-with-syphilis-treatment-failure-and-reinfection-a-longitudinal-cohort-study-in-shenzhen-china
#3
Zhenzhou Luo, Lin Zhu, Yi Ding, Jun Yuan, Wu Li, Qiuhong Wu, Lishan Tian, Li Zhang, Guomao Zhou, Tao Zhang, Jianping Ma, Zhongwei Chen, Tubao Yang, Tiejian Feng, Min Zhang
BACKGROUND: The treatment failure and reinfection rates among syphilis patients are high, and relevant studies in China are limited. The aim of this study was to detect the rates of treatment failure and reinfection after syphilis treatment and to explore the potential associated factors. METHODS: We conducted a longitudinal cohort study in a sexually transmitted disease clinic, the Department of Dermatology and Venereology in Nanshan Center for Chronic Disease Control...
September 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28889384/spheroid-cultures-of-primary-urothelial-cancer-cells-cancer-tissue-originated-spheroid-ctos-method
#4
Takahiro Yoshida, Hiroaki Okuyama, Hiroko Endo, Masahiro Inoue
Increasingly, it has been recognized that studying cancer samples from individual patients is important for the development of effective therapeutic strategies and in endeavors to overcome therapy resistance. Primary cultures of cancer cells acutely dissected from individual patients can provide a platform that enables the study and characterization of individual tumors. To that end, we have developed a method for preparing cancer cells in the form of multi-cellular spheroids. The cells can be derived from patient tumors (primary cells), from patient-derived xenografts, or from genetically- or chemically induced animal tumors...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28878072/high-throughput-protein-engineering-improves-the-antigenicity-and-stability-of-soluble-hiv-1-envelope-glycoprotein-sosip-trimers
#5
Jonathan T Sullivan, Chidananda Sulli, Alberto Nilo, Anila Yasmeen, Gabriel Ozorowski, Rogier W Sanders, Andrew B Ward, P J Klasse, John P Moore, Benjamin J Doranz
Soluble envelope glycoprotein (Env) trimers (SOSIP.664 gp140) are attractive HIV-1 vaccine candidates, with structures that mimic the native membrane-bound Env spike (gp160). Since engineering trimers can be limited by the difficulty of rationally predicting beneficial mutations, here we used a more comprehensive mutagenesis approach with the goal of identifying trimer variants with improved antigenic and stability properties. We created 341 cysteine pairs at predicted points of stabilization throughout gp140, 149 proline residue substitutions at every residue of the gp41 ectodomain, and 362 space-filling residue substitutions at every hydrophobic and aromatic residue in gp140...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28870356/the-childhood-roots-of-cardiovascular-disease-disparities
#6
REVIEW
Donald A Barr
A recent national review of the social determinants of cardiovascular disease (CVD) underscored the growing recognition that poor socioeconomic conditions early in life place children at higher risk for CVD as adults. There is growing evidence that chronic elevation of allostatic load as a consequence of high levels of early childhood stress can trigger early atherosclerotic changes in children independently of behaviors. Elevated levels of circulating cortisol have been documented in children as young as 4 years who were raised in highly stressful circumstances...
September 2017: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/28867428/epidemiological-evaluation-of-the-patient-health-questionnaire-2-in-a-pregnant-population
#7
Richelle Vlenterie, Hilde W P van Ras, Nel Roeleveld, Monica Pop-Purceleanu, Marleen M H J van Gelder
INTRODUCTION: The Patient Health Questionnaire-2 (PHQ-2) is a commonly used 2-item screening tool for depressive symptoms among pregnant women in primary care settings. However, its validity has not been assessed for large-scale epidemiological studies. Therefore, the aim of this study was to provide an epidemiological evaluation of the PHQ-2 among pregnant women. METHOD: A total of 3033 pregnant women participating in the PRegnancy and Infant DEvelopment Study completed the PHQ-2 as well as the Hospital Anxiety Depression Scale-Depression (HADS-D) or the Edinburgh Depression Scale (EDS) three times throughout pregnancy...
August 5, 2017: Journal of Psychosomatic Research
https://www.readbyqxmd.com/read/28866612/dna-repair-related-functional-assays-for-the-classification-of-brca1-and-brca2-variants-a-critical-review-and-needs-assessment
#8
REVIEW
Amanda Ewart Toland, Paul R Andreassen
Mutation of BRCA1 and BRCA2 is the most common cause of inherited breast and ovarian cancer. Genetic screens to detect carriers of variants can aid in cancer prevention by identifying individuals with a greater cancer risk and can potentially be used to predict the responsiveness of tumours to therapy. Frequently, classification cannot be performed based on traditional approaches such as segregation analyses, including for many missense variants, which are therefore referred to as variants of uncertain significance (VUS)...
September 2, 2017: Journal of Medical Genetics
https://www.readbyqxmd.com/read/28863177/identification-of-sero-reactive-antigens-for-the-early-diagnosis-of-johne-s-disease-in-cattle
#9
Lingling Li, John P Bannantine, Joseph J Campo, Arlo Randall, Yrjo T Grohn, Robab Katani, Megan Schilling, Jessica Radzio-Basu, Vivek Kapur
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease (JD), a chronic intestinal inflammatory disease of cattle and other ruminants. JD has a high herd prevalence and causes serious animal health problems and significant economic loss in domesticated ruminants throughout the world. Since serological detection of MAP infected animals during the early stages of infection remains challenging due to the low sensitivity of extant assays, we screened 180 well-characterized serum samples using a whole proteome microarray from Mycobacterium tuberculosis (MTB), a close relative of MAP...
2017: PloS One
https://www.readbyqxmd.com/read/28859154/the-cost-effectiveness-of-phq-screening-and-collaborative-care-for-depression-in-new-york-city
#10
Boshen Jiao, Zohn Rosen, Martine Bellanger, Gary Belkin, Peter Muennig
BACKGROUND: Depression is under-diagnosed and under-treated in most areas of the US. New York City is currently looking to close gaps in identifying and treating depression through the adoption of a screening and collaborative care model deployed throughout the city. METHODS: We examine the cost-effectiveness of universal two-stage screening with the 2- and 9-item Patient Health Questionnaires (PHQ-2 and PHQ-9) in New York City followed by collaborative care for those who screen positive...
2017: PloS One
https://www.readbyqxmd.com/read/28858149/conducting-clinically-based-intimate-partner-violence-research-safety-protocol-recommendations
#11
Jocelyn C Anderson, Nancy E Glass, Jacquelyn C Campbell
BACKGROUND: Maintaining safety is of utmost importance during research involving participants who have experienced intimate partner violence (IPV). Limited guidance on safety protocols to protect participants is available, particularly information related to technology-based approaches to informed consent, data collection, and contacting participants during the course of a study. PURPOSE: The purpose of the article is to provide details on the safety protocol developed and utilized with women receiving care at an urban HIV clinic and who were taking part in an observational study of IPV, mental health symptoms, and substance abuse and their relationship to HIV treatment adherence...
September 2017: Nursing Research
https://www.readbyqxmd.com/read/28842664/evaluation-of-the-elecsys-syphilis-immunoassay-for-routine-screening-of-serum-samples-in-china
#12
Chuanmin Tao, Xiaoke Hao, Wei Xu, Jie Zhang, Shiyang Pan, Zhihua Tao, Xiaofei Li, Junmei Chen, Bingchang Zhang, Yurong Qiu, Yanan Wu, Qishui Ou, Xianzhang Huang, Lanlan Wang
We compared the performance of the Roche Diagnostics Elecsys immunoassay for the detection of Treponema pallidum specific antibodies in patient serum samples with that of the Abbott Laboratories Architect chemiluminescent microparticle immunoassay and the InTec and KHB enzyme-linked immunosorbent assays, which are commonly used in China. We tested 13,767 serum samples collected from 13 independent laboratories throughout China, which included samples from 999 previously confirmed syphilis cases and 158 'borderline' samples previously identified using the Architect, InTec, and KHB tests...
August 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28835636/potential-inhibitors-designed-against-ndm-1-type-metallo-%C3%AE-lactamases-an-attempt-to-enhance-efficacies-of-antibiotics-against-multi-drug-resistant-bacteria
#13
Asad U Khan, Abid Ali, Danishuddin, Gaurava Srivastava, Ashok Sharma
NDM-1 and its variants are the most prevalent types of metallo-β-lactamases, hydrolyze almost all antibiotics of β-lactam group leading to multiple-drug resistance in bacteria. No inhibitor has yet been obtained for NDM-1 or other class of metallo-β-lactamases. Therefore, strategies to identify novel anti-β-lactamase agents with specific mechanisms of action are the need of an hour. In this study, we have reported the discovery of novel non-β-lactam inhibitors against NDM-1 by multi-step virtual screening approach...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28816097/meltblown-polymer-fabrics-as-candidate-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#14
Thomas Jenkins, Sean Meehan, Behnam Pourdeyhimi, Dianne Little
Various biomaterial technologies are promising for tissue engineering, including electrospinning, but commercial scale-up of throughput is difficult. The goal of the study was to evaluate meltblown fabrics as candidate scaffolds for rotator cuff tendon tissue engineering. Meltblown poly(lactic acid) fabrics were produced with several polymer crystallinities and airflow velocities (500(low), 900(medium) or 1400(high) m<sup>3</sup>air/hour/m fabric). Fiber diameter, alignment, and baseline bi-directional tensile mechanical properties were evaluated...
August 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28810972/when-military-fitness-standards-no-longer-apply-the-high-prevalence-of-metabolic-syndrome-in-recent-air-force-retirees
#15
Marcus M Cranston, Mark W True, Jana L Wardian, Rishawn M Carriere, Tom J Sauerwein
BACKGROUND: Metabolic syndrome (MetS) is strongly associated with cardiovascular disease. With MetS prevalence rates increasing in the U.S. population, prevention efforts have largely focused on diet and exercise interventions. Before retirement, military service members have met fitness requirements for at least 20 years, and have lower MetS rates compared to age-matched U.S. population controls (23.4% vs. 39.0%), which suggests a protective effect of the lifestyle associated with military service...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28806985/parents-and-teachers-views-on-sexual-health-education-and-screening-for-sexually-transmitted-infections-among-in-school-adolescent-girls-in-kenya-a-qualitative-study
#16
George Wanje, Linnet Masese, Ethel Avuvika, Anisa Baghazal, Grace Omoni, R Scott McClelland
BACKGROUND: To successfully develop and implement school-based sexual health interventions for adolescent girls, such as screening for Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis, it is important to understand parents' and teachers' attitudes towards sexual health education and acceptability of sexually transmitted infection (STI) screening interventions. METHODS: In this qualitative study, we approached parents and teachers from three high schools to participate in in-depth interviews (IDIs) and focus-group discussions (FGDs)...
August 14, 2017: Reproductive Health
https://www.readbyqxmd.com/read/28793149/genotypic-and-phenotypic-features-of-bap1-cancer-syndrome-a-report-of-8-new-families-and-review-of-cases-in-the-literature
#17
Alexandra M Haugh, Ching-Ni Njauw, Jeffrey A Bubley, Anna Elisa Verzì, Bin Zhang, Emily Kudalkar, Timothy VandenBoom, Kara Walton, Brian L Swick, Raj Kumar, Huma Q Rana, Sarah Cochrane, Shelley R McCormick, Christopher R Shea, Hensin Tsao, Pedram Gerami
Importance: Patients with germline mutations in BAP1 may develop several flesh-colored melanocytic BAP1-mutated atypical intradermal tumors (MBAITs). These tumors generally develop earlier than other BAP1-associated tumors, highlighting an important role for dermatologists in identifying and screening patients with a history suggestive of a germline mutation. Objective: To describe 8 new families with germline mutations in BAP1 and provide a comprehensive review of reported cases...
August 9, 2017: JAMA Dermatology
https://www.readbyqxmd.com/read/28789584/monoclonal-antibodies-technologies-for-early-discovery-and-engineering
#18
Patrick J Kennedy, Carla Oliveira, Pedro L Granja, Bruno Sarmento
Antibodies are essential in modern life sciences biotechnology. Their architecture and diversity allow for high specificity and affinity to a wide array of biochemicals. Combining monoclonal antibody (mAb) technology with recombinant DNA and protein expression links antibody genotype with phenotype. Yet, the ability to select and screen for high affinity binders from recombinantly-displayed, combinatorial libraries unleashes the true power of mAbs and a flood of clinical applications. The identification of novel antibodies can be accomplished by a myriad of in vitro display technologies from the proven (e...
August 8, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28774368/-clinical-feature-and-molecular-diagnostic-analysis-of-the-first-non-caucasian-child-with-infantile-liver-failure-syndrome-type-1
#19
Wei-Xia Lin, Qi-Qi Zheng, Li Guo, Ying Cheng, Yuan-Zong Song
Infantile liver failure syndrome type 1 (ILFS1) is a Mendelian disease due to biallelic mutations in the cytoplasmic leucyl-tRNA synthetase gene (LARS). This study aimed to report the clinical and molecular features of the first non-caucasian ILFS1 patient, providing reliable evidences for the definite diagnosis of ILFS1. The 2 years and 9 months old male patient was referred to the hospital with hepatosplenomegaly over 1 year. At age 17 months, he was found to have hepatosplenomegaly and anemia. Since then, he had been managed in different hospitals...
August 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28763500/child-contact-management-in-high-tuberculosis-burden-countries-a-mixed-methods-systematic-review
#20
Daria Szkwarko, Yael Hirsch-Moverman, Lienki Du Plessis, Karen Du Preez, Catherine Carr, Anna M Mandalakas
Tuberculosis (TB) remains a leading cause of morbidity and mortality worldwide. Considering the World Health Organization recommendation to implement child contact management (CCM) for TB, we conducted a mixed-methods systematic review to summarize CCM implementation, challenges, predictors, and recommendations. We searched the electronic databases of PubMed/MEDLINE, Scopus, and Web of Science for studies published between 1996-2017 that reported CCM data from high TB-burden countries. Protocol details for this systematic review were registered on PROSPERO: International prospective register of systematic reviews (#CRD42016038105)...
2017: PloS One
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