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https://www.readbyqxmd.com/read/28928141/a-genetic-screen-for-impaired-systemic-rnai-highlights-the-crucial-role-of-dicer-like-2
#1
Christelle Taochy, Nial R Gursanscky, Jiangling Cao, Stephen J Fletcher, Uwe Dressel, Neena Mitter, Matthew R Tucker, Anna M Koltunow, John L Bowman, Hervé Vaucheret, Bernard J Carroll
Post-transcriptional gene silencing (PTGS) of transgenes involves abundant 21 nt small interfering RNAs (siRNAs) and low abundance 22 nt siRNAs produced from double-stranded RNA (dsRNA) by DCL4 and DCL2, respectively. However, DCL2 facilitates the recruitment of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1 (AGO1)-derived cleavage products, resulting in more efficient amplification of secondary and transitive dsRNA and siRNAs. Here, we describe a reporter system where RDR6-dependent PTGS is initiated by restricted expression of an inverted-repeat dsRNA specifically in the root tip, allowing a genetic screen to identify mutants impaired in RDR6-dependent systemic PTGS...
September 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28926726/fertility-and-pregnancy-outcomes-after-transvaginal-cervico-isthmic-cerclage
#2
Marie-Emmanuelle Neveu, Hervé Fernandez, Xavier Deffieux, Marie-Victoire Senat, Marie Houllier, Perrine Capmas
INTRODUCTION: Prematurity is the leading cause of neonatal morbidity and mortality. Cervical insufficiency seems to be the main risk factor. Treatment is cervical cerclage. In case of failure, a cervico-isthmic cerclage by Fernandez' technique, with the placement of a polypropylene sling by vaginal approach during the first trimester of pregnancy, has proven its effectiveness. The aim of our study is to report effectiveness of Fernandez' cervico-isthmic cerclage in subsequent pregnancies...
September 11, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28926278/planar-optical-nano-antennas-resolve-cholesterol-dependent-nanoscale-heterogeneities-in-the-plasma-membrane-of-living-cells
#3
Raju Regmi, Pamina Martina Winkler, Valentin Flauraud, Kyra Borgman, Carlo Manzo, Juergen Brugger, Herve Rigneault, Jerome Wenger, Maria F Garcia-Parajo
Optical nano-antennas can efficiently confine light into nanoscopic hotspots enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform to investigate the dynamics of individual biomolecules in living cell membranes and their partitioning into cholesterol-dependent lipid nanodomains. Here, we present optical nano-antenna arrays with accessible surface hotspots to study the characteristic diffusion dynamics of phosphoethanolamine (PE) and sphingomyelin (SM) in the plasma membrane of living cells at the nanoscale...
September 19, 2017: Nano Letters
https://www.readbyqxmd.com/read/28924459/genetic-code-optimization-for-cotranslational-protein-folding-codon-directional-asymmetry-correlates-with-antiparallel-betasheets-trna-synthetase-classes
#4
Hervé Seligmann, Ganesh Warthi
A new codon property, codon directional asymmetry in nucleotide content (CDA), reveals a biologically meaningful genetic code dimension: palindromic codons (first and last nucleotides identical, codon structure XZX) are symmetric (CDA = 0), codons with structures ZXX/XXZ are 5'/3' asymmetric (CDA = - 1/1; CDA = - 0.5/0.5 if Z and X are both purines or both pyrimidines, assigning negative/positive (-/+) signs is an arbitrary convention). Negative/positive CDAs associate with (a) Fujimoto's tetrahedral codon stereo-table; (b) tRNA synthetase class I/II (aminoacylate the 2'/3' hydroxyl group of the tRNA's last ribose, respectively); and (c) high/low antiparallel (not parallel) betasheet conformation parameters...
2017: Computational and Structural Biotechnology Journal
https://www.readbyqxmd.com/read/28922973/a-new-wnt1-cre-tomatorosa-es-cell-line-a-tool-for-studying-neural-crest-cell-integration-capacity
#5
Soledad Acuna Mendoza, Sabrina Martin, Sabine Kuchler-Bopp, Sandy Ribes, Jeremy H Thalgott, Catherine Chaussain, Sophie Creuzet, Hervé Lesot, Franck Lebrin, Anne Poliard
Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies but further knowledge on their in vivo behaviour is required before clinical transfer may be envisioned. We here describe isolation and characterization of a new mouse ES line derived from Wnt1-CRE-R26 RosaTomatoTdv blastocyst and show that it displays the characteristics of typical ES cells. Furthermore, these cells can be efficiently directed towards a NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence...
September 19, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28922120/motion-estimation-in-echocardiography-using-sparse-representation-and-dictionary-learning
#6
Nora Ouzir, Adrian Basarab, Herve Liebgott, Brahim Harbaoui, Jean-Yves Tourneret
This paper introduces a new method for cardiac motion estimation in 2D ultrasound images. The motion estimation problem is formulated as an energy minimization, whose data fidelity term is built using the assumption that the images are corrupted by multiplicative Rayleigh noise. In addition to a classical spatial smoothness constraint, the proposed method exploits the sparse properties of the cardiac motion to regularize the solution via an appropriate dictionary learning step. The proposed method is evaluated on one dataset with available ground-truth, including four sequences of highly realistic simulations...
September 18, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28920574/status-quality-and-specific-needs-of-zika-virus-zikv-diagnostic-capacity-and-capability-in-national-reference-laboratories-for-arboviruses-in-30-eu-eea-countries-may-2016
#7
Ramona Mögling, Hervé Zeller, Joana Revez, Marion Koopmans, Chantal Reusken
With international travel, Zika virus (ZIKV) is introduced to Europe regularly. A country's ability to robustly detect ZIKV introduction and local transmission is important to minimise the risk for a ZIKV outbreak. Therefore, sufficient expertise and diagnostic capacity and capability are required in European laboratories. To assess the capacity, quality, operational specifics (guidelines and algorithms), technical and interpretation issues and other possible difficulties that were related to ZIKV diagnostics in European countries, a questionnaire was conducted among national reference laboratories in 30 countries in the European Union/European Economic Area (EU/EEA) in May 2016...
September 7, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28920118/hybrid-magnetic-iron-oxide-nanoparticles-with-tunable-field-directed-self-assembly
#8
Vikash Malik, Antara Pal, Olivier Pravaz, Jérôme J Crassous, Simon Granville, Bernard Grobety, Ann M Hirt, Hervé Dietsch, Peter Schurtenberger
We describe the synthesis of hybrid magnetic ellipsoidal nanoparticles that consist of a mixture of two different iron oxide phases, hematite (α-Fe2O3) and maghemite (γ-Fe2O3), and characterize their magnetic field-driven self-assembly. We demonstrate that the relative amount of the two phases can be adjusted in a continuous way by varying the reaction time during the synthesis, leading to strongly varying magnetic properties of the particles. Not only does the saturation magnetization increase dramatically as the composition of the spindles changes from hematite to maghemite, but also the direction of the induced magnetic moment changes from being parallel to the short axis of the spindle to being perpendicular to it...
September 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28919068/intubation-and-extubation-of-the-icu-patient
#9
Hervé Quintard, Erwan l'Her, Julien Pottecher, Frédéric Adnet, Jean-Michel Constantin, Audrey Dejong, Pierre Diemunsch, Rose Fesseau, A Freynet, Christophe Girault, Christophe Guitton, Y Hamonic, Eric Maury, Armand Mekontso-Dessap, F Michel, P Nolent, Sébastien Perbet, Gwénaël Prat, Antoine Roquilly, Karim Tazarourte, Nicolas Terzi, Arnaud W Thille, Mikael Alves, Etienne Gayat, Laurence Donetti
No abstract text is available yet for this article.
September 14, 2017: Anaesthesia, Critical Care & Pain Medicine
https://www.readbyqxmd.com/read/28919067/impact-of-iron-deficiency-diagnosis-using-hepcidin-mass-spectrometry-dosage-methods-on-hospital-stay-and-costs-after-a-prolonged-icu-stay-study-protocol-for-a-multicentre-randomised-single-blinded-medico-economic-trial
#10
Sigismond Lasocki, Hervé Puy, Grégoire Mercier, Sylvain Lehmann
BACKGROUND: Iron deficiency (ID) is frequent but difficult to diagnose in critically ill patients. ID may be responsible for prolonged post-ICU hospital stays, since it results in fatigue, muscle weakness and anaemia. Hepcidin, the key iron metabolism hormone, may be a good marker of ID in these patients. The aim of this study is to determine whether using mass spectrometry hepcidin determination to diagnose (and treat) ID after prolonged ICU stays may reduce patients' subsequent hospital stays and costs in comparison with conventional (ferritin) methods...
September 14, 2017: Anaesthesia, Critical Care & Pain Medicine
https://www.readbyqxmd.com/read/28918209/treatment-of-postoperative-distal-anastomotic-endoleak-with-the-amplatzer-vascular-plug-ii-in-chronic-aortic-arch-dissection-a-case-report
#11
Iskander Bouassida, Olivier Meyrignac, Bertrand Marcheix, Paul Revel-Mouroz, Hervé Rousseau
No abstract text is available yet for this article.
August 24, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28917882/a-review-about-brown-algal-cell-walls-and-fucose-containing-sulfated-polysaccharides-cell-wall-context-biomedical-properties-and-key-research-challenges
#12
REVIEW
Estelle Deniaud-Bouët, Kevin Hardouin, Philippe Potin, Bernard Kloareg, Cécile Hervé
Studies on brown algal cell walls have entered a new phase with the concomitant discovery of novel polysaccharides present in cell walls and the establishment of a comprehensive generic model for cell wall architecture. Brown algal cell walls are composites of structurally complex polysaccharides. In this review we discuss the most recent progress in the structural composition of brown algal cell walls, emphasizing the significance of extraction and screening techniques, and the biological activities of the corresponding polysaccharides, with a specific focus on the fucose-containing sulfated polysaccharides...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28916823/digging-into-the-low-molecular-weight-peptidome-with-the-oligonet-web-server
#13
Youzhong Liu, Sara Forcisi, Marianna Lucio, Mourad Harir, Florian Bahut, Magali Deleris-Bou, Sibylle Krieger-Weber, Régis D Gougeon, Hervé Alexandre, Philippe Schmitt-Kopplin
Bioactive peptides play critical roles in regulating many biological processes. Recently, natural short peptides biomarkers are drawing significant attention and are considered as "hidden treasure" of drug candidates. High resolution and high mass accuracy provided by mass spectrometry (MS)-based untargeted metabolomics would enable the rapid detection and wide coverage of the low-molecular-weight peptidome. However, translating unknown masses (<1 500 Da) into putative peptides is often limited due to the lack of automatic data processing tools and to the limit of peptide databases...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901136/tren-capped-hexaphyrin-zinc-complexes-interplaying-molecular-recognition-m%C3%A3-bius-aromaticity-and-chirality
#14
Hervé Ruffin, Gildas Nyame Mendendy Boussambe, Thierry Roisnel, Vincent Dorcet, Bernard Boitrel, Stéphane Le Gac
Over the last decade, the hexaphyrin skeleton has emerged as a multifaceted frame exhibiting strong interplay between topology, aromaticity and metal coordination, opening new research areas beyond porphyrins. Yet, molecular recognition with hexaphyrins has been under-explored, mainly due to the lack of general synthetic strategies leading to sophisticated molecular hosts. Here, we have developed a straightforward approach for capping the heteroannulene frame with tripodal units (e.g., tris(2-aminoethyl)amine [tren]), through the post-modification of a readily accessible meso-(2-aminophenyl) tris-substituted platform...
September 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28899104/therapeutic-vaccine-against-hiv-viral-variability-ctl-epitopes-and-genetics-of-patients
#15
Herve J Fleury, Camille Tumiotto, Pantxika Bellecave, Patricia Recordon-Pinson
The scientific and medical community is seeking to cure HIV. Several pathways have been or are being explored including therapeutic vaccination. Viro-immunological studies on primary infection as well as on elite controllers have demonstrated the importance of the cytotoxic CD8 response and have mainly oriented research on vaccine constructs towards this type of response. The results of these trials are clearly not commensurate with the hope placed in them. Might there be one or more uncontrolled variables? The genetics of patients need to be taken into consideration, especially their HLA alleles...
September 13, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28895752/airflow-obstruction-and-use-of-solid-fuels-for-cooking-or-heating-bold-results
#16
André F S Amaral, Jaymini Patel, Bernet S Kato, Daniel O Obaseki, Hervé Lawin, Wan C Tan, Sanjay K Juvekar, Imed Harrabi, Michael Studnicka, Emiel F M Wouters, Li-Cher Loh, Eric D Bateman, Kevin Mortimer, A Sonia Buist, Peter G J Burney
RATIONALE: Evidence supporting the association of COPD or airflow obstruction with use of solid fuels is conflicting and inconsistent. OBJECTIVE: To assess the association of airflow obstruction with self-reported use of solid fuels for cooking or heating. METHODS: We analysed 18,554 adults from the BOLD study, who had provided acceptable post-bronchodilator spirometry measurements and information on use of solid fuels. The association of airflow obstruction with use of solid fuels for cooking or heating was assessed by sex, within each site, using regression analysis...
September 12, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28894984/multifidelity-cma-a-multifidelity-approach-for-efficient-personalisation-of-3d-cardiac-electromechanical-models
#17
Roch Molléro, Xavier Pennec, Hervé Delingette, Alan Garny, Nicholas Ayache, Maxime Sermesant
Personalised computational models of the heart are of increasing interest for clinical applications due to their discriminative and predictive abilities. However, the simulation of a single heartbeat with a 3D cardiac electromechanical model can be long and computationally expensive, which makes some practical applications, such as the estimation of model parameters from clinical data (the personalisation), very slow. Here we introduce an original multifidelity approach between a 3D cardiac model and a simplified "0D" version of this model, which enables to get reliable (and extremely fast) approximations of the global behaviour of the 3D model using 0D simulations...
September 11, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28894286/intermittent-hypoxia-induced-insulin-resistance-is-associated-with-alterations-in-white-fat-distribution
#18
Laureline Poulain, Hervé Mathieu, Amandine Thomas, Anne-Laure Borel, Chantal Remy, Patrick Levy, Claire Arnaud, Maurice Dematteis
Sleep apnea syndrome is characterized by repetitive upper airway collapses during night leading to intermittent hypoxia (IH). The latter is responsible for metabolic disturbances that rely, at least in part, on abdominal white fat inflammation. Besides qualitative alterations, we hypothesized that IH could also modify body fat distribution, a key factor for metabolic complications. C57BL6 mice exposed to IH (21-5% FiO2, 60 s cycle, 8 h/day) or air for 6 weeks were investigated for topographic fat alterations (whole-body MRI)...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28893944/making-a-virtue-of-necessity-the-pleiotropic-role-of-human-endogenous-retroviruses-in-cancer
#19
REVIEW
George Kassiotis, Jonathan P Stoye
Like all other mammals, humans harbour an astonishing number of endogenous retroviruses (ERVs), as well as other retroelements, embedded in their genome. These remnants of ancestral germline infection with distinct exogenous retroviruses display various degrees of open reading frame integrity and replication capability. Modern day exogenous retroviruses, as well as the infectious predecessors of ERVs, are demonstrably oncogenic. Further, replication-competent ERVs continue to cause cancers in many other species of mammal...
October 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28893857/changes-in-dynamic-lung-mechanics-after-lung-volume-reduction-coil-treatment-of-severe-emphysema
#20
Demosthenes Makris, Sylvie Leroy, Johana Pradelli, Jonathan Benzaquen, Hervé Guenard, Jeanne-Marie Perotin, Spyros Zakynthinos, Epaminondas Zakynthinos, Gaëtan Deslee, Charles Hugo Marquette
We assessed the relationships between changes in lung compliance, lung volumes and dynamic hyperinflation in patients with emphysema who underwent bronchoscopic treatment with nitinol coils (coil treatment) (n=11) or received usual care (UC) (n=11). Compared with UC, coil treatment resulted in decreased dynamic lung compliance (CLdyn) (p=0.03) and increased endurance time (p=0.010). The change in CLdyn was associated with significant improvement in FEV1 and FVC, with reduction in residual volume and intrinsic positive end-expiratory pressure, and with increased inspiratory capacity at rest/and at exercise...
September 11, 2017: Thorax
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