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https://www.readbyqxmd.com/read/28070012/prevalence-and-risk-factors-for-intestinal-parasitic-infection-in-schoolchildren-in-battambang-cambodia
#1
Chien-Wei Liao, Kuan-Chih Chiu, I-Chen Chiang, Po-Ching Cheng, Ting-Wu Chuang, Juo-Han Kuo, Yun-Hung Tu, Chia-Kwung Fan
Most intestinal parasitic infections (IPIs), commonly endemic in tropical resource-poor developing countries, are neglected tropical diseases. Parasitic infections and malnutrition are most commonly found in children. We determined the prevalence of IPIs and the risk factors in Battambang Province, northwestern Cambodia, from August to September 2015. This study collected 308 valid questionnaires and specimens from Dontri (173, 56.2%) and Kon Kaêk (135, 43.8%) primary schools. All stool samples were examined using Chang's Feces Examination Apparatus through the merthiolate-iodine-formaldehyde technique...
January 9, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28049541/the-presence-of-giardia-intestinalis-in-donkeys-equus-asinus-in-china
#2
Xiao-Xuan Zhang, Fu-Kai Zhang, Fa-Cai Li, Jun-Ling Hou, Wen-Bin Zheng, Shuai-Zhi Du, Quan Zhao, Xing-Quan Zhu
BACKGROUND: Giardia intestinalis is one of the most important zoonotic enteric parasites. As no information regarding prevalence and genotype of G. intestinalis in donkeys (Equus asinus) in China is available, 181 faecal samples from 48 donkeys from Jilin Province, from 104 from Shandong Province and from 29 from Liaoning Province were examined between May and December 2015. FINDINGS: Twenty-eight (15.47%) out of 181 donkey samples were tested G. intestinalis-positive by nested amplification of the triosephosphate isomerase (tpi) gene...
January 3, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28030941/diagnostics-of-intestinal-parasites-in-light-microscopy-among-the-population-of-children-in-eastern-afghanistan
#3
Krzysztof Korzeniewski, Agata Smoleń, Alina Augustynowicz, Anna Lass
OBJECTIVES: The Afghans, living in poor socioeconomic conditions, are estimated to be a community with a high rate of intestinal parasitic infections. The aim of the study was to estimate the prevalence and species of intestinal parasites among children's population in eastern Afghanistan and to present the methods of optimizing the techniques for identification of pathogens in light microscopy. The research was carried out as a part of humanitarian project Capacity building of health care system in Ghazni Province...
23, 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28030344/report-of-fatal-mixed-infection-with-cryptosporidium-parvum-and-giardia-intestinalis-in-neonatal-calves
#4
Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai
In the production and management of beef and dairy cattle, controlling diarrhea is one of the important concerns. Pathogenic agents of the disease, protozoan parasites including Cryptosporidium spp., are difficult to control, making prevention, diagnoses, and treatment of diarrhea. In the present study, we investigated a farm with a history of calf deaths over a period of 10 years in order to determine the cause of disease and to clarify the detailed distribution of the pathogens. In four examined calves that were reared in calf pens, all were positive with Cryptosporidium and/or Giardia, while the other breeding stock and adult cattle were negative...
March 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28009386/detection-of-giardia-intestinalis-infections-in-polish-soldiers-deployed-to-afghanistan
#5
Krzysztof Korzeniewski, Monika Konior, Alina Augustynowicz, Anna Lass, Ewa Kowalska
BACKGROUND: Members of the Polish Military Contingent (PMC) have been stationed in Afghanistan since 2002. They typically serve in areas characterised by low standards of sanitation which often leads to the development of food- and waterborne diseases. The aim of the study was to evaluate the prevalence of Giardia intestinalis infections among Polish soldiers deployed to Afghanistan. The research study was conducted as part of a programme for prevention of parasitic diseases of the gastrointestinal tract run by the Polish Armed Forces...
2016: International Maritime Health
https://www.readbyqxmd.com/read/28007484/diagnosis-of-intestinal-parasites-in-a-rural-community-of-venezuela-advantages-and-disadvantages-of-using-microscopy-or-rt-pcr
#6
Renzo Nino Incani, Elizabeth Ferrer, Denise Hoek, Robbert Ramak, Jeroen Roelfsema, Lapo Mughini-Gras, Titia Kortbeek, Elena Pinelli
A cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time PCR (RT-PCR) for 14 intestinal parasites in a Venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. A total of 228 participants were included in this study. A multiplex RT-PCR was used for the detection of Dientamoeba fragilis, Giardia intestinalis, Cryptosporidium sp. and a monoplex RT-PCR for Entamoeba histolytica...
December 19, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27923058/effect-of-mass-stool-examination-and-mass-treatment-for-decreasing-intestinal-helminth-and-protozoan-infection-rates-in-bolivian-children-a-cross-sectional-study
#7
Takao Asai, Claudia Còrdova Vidal, Wilma Strauss, Toshikazu Ikoma, Kazuo Endoh, Masaharu Yamamoto
Bolivia is one of the countries with a high intestinal helminth and protozoan infection rate. Despite the high prevalence of the parasitic infection, nationwide preventive measures for Bolivian children have not yet been implemented. We evaluated the effect of mass stool examination and treatment as a strategy for decreasing the infection rate. This study was conducted between 2013 and 2015 in children aged 2-18 years. A total of 2,033 stool samples (575 in 2013, 815 in 2014 and 642 in 2015) were collected and examined using the formalin-ether medical sedimentation method...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27905282/distribution-of-parasites-detected-in-stool-samples-of-patients-admitted-to-our-parasitology-laboratory-during-a-three-year-period-between-2012-and-2014
#8
Mehmet Burak Selek, Bayhan Bektöre, Ergenekon Karagöz, Orhan Baylan, Mustafa Özyurt
OBJECTIVE: Parasitic diseases are among the major public health issues worldwide. A number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. Nevertheless, the most common diagnostic method is microscopic examination. In this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 2014...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27876969/molecular-detection-of-giardiasis-among-children-at-cairo-university-pediatrics-hospitals
#9
Marwa A Ghieth, Magd A Kotb, Enas Y Abu-Sarea, Ayman A El-Badry
Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27871871/paleoparasitological-analysis-of-the-extinct-myotragus-balearicus-bate-1909-artiodactyla-caprinae-from-mallorca-balearic-islands-western-mediterranean
#10
Victor Hugo Borba Nunes, Josep Antoni Alcover, Valmir Laurentino Silva, Paula Borba Cruz, José Roberto Machado-Silva, Adauto José Gonçalves de Araújo
Myotragus balearicus (Artiodactyla, Caprinae) is an extinct caprine endemic of the Eastern Balearic Islands or Gymnesics (i.e., Mallorca, Menorca and surrounding islets, Western Mediterranean Sea). In spite of its small size, c. 50cm height at the shoulder, it was the largest mammal inhabiting these islands until the human arrival, and it had peculiar short legs and frontal vision. It disappeared between 2830 and 2210calBCE. The coprolites here studied were recovered from Cova Estreta, in Pollença, Mallorca...
November 18, 2016: Parasitology International
https://www.readbyqxmd.com/read/27821451/phase-i-clinical-trial-results-of-auranofin-a-novel-antiparasitic-agent
#11
Edmund V Capparelli, Robin Bricker-Ford, M John Rogers, James H McKerrow, Sharon L Reed
Under an NIH priority to identify new drugs to treat class B parasitic agents, we performed high-throughput screens, which identified the activity of auranofin (Ridaura) against Entamoeba histolytica and Giardia intestinalis, major causes of water- and foodborne outbreaks. Auranofin, an orally administered, gold (Au)-containing compound that was approved by the FDA in 1985 for treatment of rheumatoid arthritis, was effective in vitro and in vivo against E. histolytica and both metronidazole-sensitive and -resistant strains of Giardia We now report the results of an NIH-sponsored phase I trial to characterize the pharmacokinetics (PK) and safety of auranofin in healthy volunteers using modern techniques to measure gold levels...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27815907/synchronization-of-pathogenic-protozoans
#12
Staffan Svärd, Karin Troell
Protozoans are single-celled eukaryotes and many of the best-studied protozoans are parasitic to humans (e.g., Plasmodium falciparum causing malaria and Trypanosoma brucei causing sleeping sickness). These organisms are distantly related to humans but with retained eukaryotic type of cellular processes, making them good model systems for studies of the evolution of basic processes like the cell cycle. Giardia intestinalis causes 250 million cases of diarrhea yearly and is one of the earliest diverging protozoans...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27756339/prevalence-of-intestinal-parasitic-infections-and-associated-risk-factors-among-schoolchildren-in-the-plateau-central-and-centre-ouest-regions-of-burkina-faso
#13
Séverine Erismann, Serge Diagbouga, Peter Odermatt, Astrid M Knoblauch, Jana Gerold, Akina Shrestha, Tarnagda Grissoum, Aminata Kaboré, Christian Schindler, Jürg Utzinger, Guéladio Cissé
BACKGROUND: Unsafe drinking water, unimproved sanitation and lack of hygiene pose health risks, particularly to children in low- and middle-income countries. This study aimed to assess the prevalence and risk factors of intestinal parasitic infections in school-aged children in two regions of Burkina Faso. METHODS: A cross-sectional survey was carried out in February 2015 with 385 children aged 8-14 years from eight randomly selected schools in the Plateau Central and Centre-Ouest regions of Burkina Faso...
October 18, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27717905/anti-giardia-activity-and-acute-toxicity-of-a-methanol-extract-of-senna-racemosa-bark
#14
Edgar E Caamal-Fuentes, Manlio Graniel-Sabido, Gonzalo J Mena-Rejón, Rosa E Moo-Puc
ETHNOPHARMACOLOGICAL RELEVANCE: Senna racemosa (Mill.) H.S. Irwin & Barneby (syn. Cassia racemosa Mill.) is a plant used in traditional Mayamedicinal practices to treat diarrhea. A methanol extract of S. racemosa bark has been shown to have in vitro activity against Giardia intestinalis. No studies of its efficacy and toxicity in in vivo models have been done. The present study objective was to analyze the activity of this methanol extract of S. racemosa bark against Giardia intestinalis trophozoites in experimentally infected mice, and evaluate its toxicological effects in rats...
December 4, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27660859/molecular-identification-of-giardia-duodenalis-isolates-from-domestic-dogs-and-cats-in-wroclaw-poland
#15
Jolanta Piekarska, Joanna Bajzert, Michał Gorczykowski, Magdalena Kantyka, Magdalena Podkowik
INTRODUCTION: Giardia duodenalis (G. intestinalis) is a common protozoan causing gastrointestinal disorders in many species of mammals. The genus of Giardia has high molecular diversity. Dogs and cats, in addition to their typical infection with assemblages C, D and F, may be a reservoir of zoonotic assemblages (A and B). OBJECTIVE: The aim of this study was a genetic characteristic of Giardia isolates of dogs and cats from the area of Wroclaw (Poland). MATERIALS AND METHOD: A total of 128 and 33 faecal samples from dogs and cats, respectively, were analyzed by routine coprological methods...
September 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/27638793/occurrence-of-giardia-intestinalis-and-cryptosporidium-sp-in-wastewater-samples-from-s%C3%A3-o-paulo-state-brazil-and-lima-peru
#16
Francisco Miroslav Ulloa-Stanojlović, Bruna Aguiar, Luis M Jara, Maria Inês Zanoli Sato, Juana Arzola Guerrero, Elayse Hachich, Glavur Rogério Matté, Milena Dropa, Maria Helena Matté, Ronalda Silva de Araújo
The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of São Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis...
September 9, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27638151/characterization-of-telomeres-and-telomerase-from-the-single-celled-eukaryote-giardia-intestinalis
#17
Magdalena Uzlíková, Jana Fulnečková, Filip Weisz, Eva Sýkorová, Eva Nohýnková, Pavla Tůmová
The ends of linear chromosomes, telomeres, are most commonly maintained by the enzyme telomerase. Our study presents the characteristics of telomeres and telomerase from the single-celled parasitic eukaryote Giardia intestinalis. Using fluorescence in situ hybridization, we localized telomeres during all stages of the trophozoite cell cycle and demonstrated differences in the observed number of telomeric foci, indicating telomere clustering. The length of Giardia telomeres was determined in different cell lines derived from WB clinical isolate using terminal restriction fragment analysis and ranged from 0...
January 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/27622633/partial-inhibition-of-two-genes-that-encode-spliceosomal-proteins-in-giardia-intestinalis
#18
Vanessa Gómez, Moisés Wasserman
Giardia intestinalis is an early divergent organism that was recently shown to have introns. The machinery responsible for the removal of introns in higher eukaryotes is the spliceosome, which consists of five ribonucleoproteins. Each of these ribonucleoproteins has a small nuclear RNA, a set of seven Sm proteins (B, D1, D2, D3, E, F and G) and several specific proteins. Some genes that encode spliceosome proteins have been bioinformatically identified in the parasite genome. Although it is assumed that the spliceosome is responsible for splicing in this parasite, biochemical characterization is lacking...
March 29, 2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27608965/mitosomal-chaperone-modulation-during-the-life-cycle-of-the-pathogenic-protist-giardia-intestinalis
#19
Victor Midlej, Luciana Penha, Rosane Silva, Wanderley de Souza, Marlene Benchimol
The mitosome is a double-membrane enveloped organelle that is found in few unicellular eukaryotes, one of which is the human intestinal parasitic protist Giardia intestinalis, which also lacks mitochondria and peroxisomes. This flagellated protist grows in vitro as trophozoites and under some conditions, differentiates into cysts, which are characterized by the absence of externalized flagella, a round shape, and the presence of a cyst wall. The presence and distribution of mitosomal proteins, such as giardial iron-sulfur cluster protein (GiIscU), heat-shock protein 70 (mit-HSP70) and giardial chaperonin 60 (GiCpn60), during the process of trophozoite-to-cyst transformation was tracked using confocal laser scanning microscopy and western blotting...
December 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/27605751/comparison-of-microscopic-and-immunoassay-examination-in-the-diagnosis-of-intestinal-protozoa-of-humans-in-mansoura-egypt
#20
Shaadi F Elswaifi, James R Palmieri, Nora El-Tantawy, Mona El-Hussiny, Tarek Besheer, Ekbal Abohashem
Protozoal diseases are prevalent globally and especially in developing countries that have relatively lower socioeconomic populations such as Egypt. Direct microscopic examination (DME) is used for the detection and identification of protozoa but lacks sufficient reliability, and thus may be detrimental in obtaining accurate diagnostic or epidemiological data. In this study, we determine the prevalence of infections by Giardia intestinalis, Cryptosporidium sp., and Entamoeba histolytica in humans in Egypt. Furthermore, we determine the reliability of DME in determining infections caused by these protozoa and compare the results to enzyme linked Immunosorbent assays (ELISA)...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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