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Giardia intestinalis

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https://www.readbyqxmd.com/read/29351067/changes-in-the-incidence-of-intestinal-giardiosis-in-mexican-population-during-five-years-2011-2015
#1
Gabriela Ibáñez-Cervantes, Gloria León-Ávila, Juan Manuel Bello-López, Armando Pérez-Rangel, Gregorio León-García, Benjamín Nogueda-Torres, José Manuel Hernández
Giardiosis is a parasitic disease caused by the protozoan Giardia intestinalis, which is distributed worldwide. Most of the data on the prevalence of giardiosis in Mexico comes from research, but it is also necessary to study the data provided by the Mexican Health Ministry and issued by the General Directorate of Epidemiology. The aim of this work was analyse the national surveillance data for human giardiosis in order to update the epidemiological data of this disease in Mexico. A retrospective observational analysis of giardiosis (from January 2011 to December 2015) was performed in the annual reports emitted by the GDE in Mexico...
March 26, 2018: Acta Parasitologica
https://www.readbyqxmd.com/read/29313513/diseases-due-to-intestinale-protozoa-diagnosed-at-le-dantec-university-hospital-in-dakar-senegal-from-2011-to-2015
#2
K Diongue, M Ndiaye, M C Seck, M A Diallo, Y D Ndiaye, A S Badiane, D Ndiaye
To identify the protozoa responsible for intestinal parasitosis diagnosed at Le Dantec University Hospital in Dakar, Senegal. The retrospective study included 2578 patients for whom stool samples were analyzed from 2011 to 2015. The sex ratio was 1.1. Patients' mean age was 28.5 years, with a range of 11 days to 91 years. Direct examination and the Ritchie technique were performed to search for parasites. In total, 317 patients had intestinal protozoa, for a prevalence of 12.3 %. Infections involved one (91%) or two parasites (9%)...
November 1, 2017: Médecine et Santé Tropicales
https://www.readbyqxmd.com/read/29286011/prevalence-of-zoonotic-and-non-zoonotic-genotypes-of-giardia-intestinalis-in-cats-a-systematic-review-and-meta-analysis
#3
Sebastián Ramírez-Ocampo, Juan David Cotte-Alzate, Ángel A Escobedo, Alfonso J Rodríguez-Morales
There are no meta-analyses specifically describing the prevalence of zoonotic and non-zoonotic genotypes of Giardia intestinalis in cats, which would be useful in defining the importance of cats as a source of zoonotic transmission. We performed a systematic review of the literature in three databases (PubMed, Scopus and SciELO) to assess the proportion of cats that were infected with specific G. intestinalis genotypes. A meta-analysis using a random effects model was performed to calculate the pooled prevalence and 95% confidence intervals (95%CI)...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29261753/prevalence-and-molecular-characterization-of-giardia-intestinalis-in-racehorses-from-the-sichuan-province-of-southwestern-china
#4
Lei Deng, Wei Li, Zhijun Zhong, Xuehan Liu, Yijun Chai, Xue Luo, Yuan Song, Wuyou Wang, Chao Gong, Xiangming Huang, Yanchun Hu, Hualin Fu, Min He, Ya Wang, Yue Zhang, Kongju Wu, Suizhong Cao, Guangneng Peng
Giardia intestinalis, a cosmopolitan zoonotic parasite, is one of the most common causes of protozoal diarrhea in both humans and animals worldwide. Although G. intestinalis has been detected in many animals, information regarding its prevalence and genotype in Chinese racehorses is scarce. In the present study, we investigated the prevalence of G. intestinalis in racehorses and performed molecular characterization of the pathogen to assess its zoonotic potential. Two hundred and sixty-four racehorse fecal samples from six equestrian clubs located in different regions of the Sichuan province of southwestern China were examined...
2017: PloS One
https://www.readbyqxmd.com/read/29260648/enteroparasites-in-preschool-children-on-the-pacific-region-of-nicaragua
#5
Carla Muñoz-Antoli, Mónica Gozalbo, Aleyda Pavón, Paloma Escobedo, Rafael Toledo, Jose-Guillermo Esteban
The aim of this study was to determine for the first time the prevalence of enteroparasites in preschool children originating from the seven departments of the Pacific region in Nicaragua. One stool sample of each of 1,217 children, from 6 months to 5 years of age, was collected and personal data were recorded on delivery of the container. Samples fixed in 10% formalin were processed by a formol-acetate concentration and a modified Ziehl-Neelsen technique. The overall prevalence of enteroparasite infections was 68...
December 18, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29228011/characterization-of-the-giardia-intestinalis-secretome-during-interaction-with-human-intestinal-epithelial-cells-the-impact-on-host-cells
#6
Showgy Y Ma'ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G Svärd
BACKGROUND: Giardia intestinalis is a non-invasive protozoan parasite that causes giardiasis in humans, the most common form of parasite-induced diarrhea. Disease mechanisms are not completely defined and very few virulence factors are known. METHODOLOGY: To identify putative virulence factors and elucidate mechanistic pathways leading to disease, we have used proteomics to identify the major excretory-secretory products (ESPs) when Giardia trophozoites of WB and GS isolates (assemblages A and B, respectively) interact with intestinal epithelial cells (IECs) in vitro...
December 11, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29214976/stray-cats-are-more-frequently-infected-with-zoonotic-protists-than-pet-cats
#7
Martin Kvac, Lada Hofmannova, Ynes Ortega, Nikola Holubova, Michaela Horcickova, Marta Kicia, Lenka Hlaskova, Dana Kvetonova, Bohumil Sak, John McEvoy
Faecal samples were collected from cats kept as pets (n = 120) and stray cats (n = 135) in Central Europe (Czech Republic, Poland and Slovakia) and screened for the presence of Cryptosporidium spp., Giardia intestinalis (Kunstler, 1882), Encephalitozoon spp. and Enterocytozoon bieneusi Desportes, Le Charpentier, Galian, Bernard, Cochand-Priollet, Lavergne, Ravisse et Modigliani, 1985 by PCR analysis of the small-subunit of rRNA (Cryptosporidium spp. and G. intestinalis) and ITS (microsporidia) genes. Sequence analysis of targeted genes revealed the presence of C...
December 6, 2017: Folia Parasitologica
https://www.readbyqxmd.com/read/29168467/temporal-trends-of-intestinal-parasites-in-patients-attending-a-tertiary-care-hospital-in-south-india-a-seven-year-retrospective-analysis
#8
Ira Praharaj, Rajiv Sarkar, Sitara Swarna Rao Ajjampur, Sheela Roy, Gagandeep Kang
BACKGROUND & OBJECTIVES: Intestinal parasitic infections and their associated complications are a major cause of morbidity in the developing world. This retrospective study was done to assess the prevalence of intestinal parasitic infections among patients in a tertiary healthcare setting and to analyze age-, gender- and time-related trends in the prevalence of these intestinal parasites over a seven year period (2006-2012). METHODS: The presence of various intestinal parasites in a tertiary care setting over a seven year period in different age groups was determined by performing routine stool microscopy...
July 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29156877/comparison-of-microscopy-elisa-and-real-time-pcr-for-detection-of-giardia-intestinalis-in-human-stool-specimens
#9
Yunus Emre Beyhan, Zeynep Taş Cengiz
Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-ether sedimentation technique and microscopic examinations were carried out on wet mount slides. A commercially available ELISA kit (Giardia CELISA, Cellabs, Brookvale, Australia) was used for immunoassay...
August 23, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29155829/environmental-and-socio-demographic-individual-family-and-neighborhood-factors-associated-with-children-intestinal-parasitoses-at-iguaz%C3%A3%C2%BA-in-the-subtropical-northern-border-of-argentina
#10
Maria Romina Rivero, Carlos De Angelo, Pablo Nuñez, Martín Salas, Carlos E Motta, Alicia Chiaretta, Oscar D Salomón, Song Liang
BACKGROUND: Intestinal parasitoses are a major concern for public health, especially in children from middle and low-income populations of tropical and subtropical areas. We examined the presence and co-infection of parasites in humans as well as parasitic environmental contamination in Puerto Iguazú, Argentina. We explored the environmental and socio-demographic characteristics of the persistence of parasites in children and their environment. METHODOLOGY/PRINCIPAL FINDINGS: This cross-section survey was conducted among children population comprised into the area of the public health care centers of Iguazú during June 2013 to May 2016...
November 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29154846/effect-of-piper-betle-on-giardia-intestinalis-infection-in%C3%A2-vivo
#11
Radka Pecková, Karel Doležal, Bohumil Sak, Dana Květoňová, Martin Kváč, Wisnu Nurcahyo, Ivona Foitová
Piper betle has been used as a medicinal plant in traditional medical systems throughout South and South East Asia. Experimental studies have revealed its wide and diverse biological and pharmacological effects. In this study, antigiardial activity of Piper betle was tested using experimental infections of Giardia intestinalis, the most common cause of protozoal diarrhoea worldwide, in Mongolian gerbils. Plants were extracted in water, methanol and methanol:tetrahydrofuran. Gerbils were treated for ten days intragastrically twice a day, with the dose of 40 mg of the extract per 100 g of body weight...
November 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29066287/multiple-identification-of-most-important-waterborne-protozoa-in-surface-water-used-for-irrigation-purposes-by-18s-rrna-amplicon-based-metagenomics
#12
Y Moreno, L Moreno-Mesonero, I Amorós, R Pérez, A J Morillo, J L Alonso
Understanding waterborne protozoan parasites (WPPs) diversity has important implications in public health. In this study, we evaluated a NGS-based method as a detection approach to identify simultaneously most important WPPs using 18S rRNA high-throughput sequencing. A set of primers to target the V4 18S rRNA region of WPPs such as Cryptosporidium spp., Giardia sp., Blastocystis sp., Entamoeba spp, Toxoplasma sp. and free-living amoebae (FLA) was designed. In order to optimize PCR conditions before sequencing, both a mock community with a defined composition of representative WPPs and a real water sample inoculated with specific WPPs DNA were prepared...
October 19, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29041984/prevalence-and-multilocus-genotyping-of-giardia-duodenalis-in-pigs-of-shaanxi-province-northwestern-china
#13
Sha-Sha Wang, Ya-Jie Yuan, Yan-Ling Yin, Rui-Si Hu, Jun-Ke Song, Guang-Hui Zhao
BACKGROUND: Giardiasis, caused by Giardia duodenalis (syn. Giardia intestinalis, Giardia lamblia), is a significant zoonotic parasitic disease of animals and humans worldwide. Accurate genotyping of G. duodenalis is essential for efficient control and management of giardiasis. The objectives of the present study were to investigate the prevalence and assemblages of giardiasis in pigs in Shaanxi Province, northwestern China, and for the first time study multilocus genotypes (MLGs) in pigs using multilocus genotyping technology in this region...
October 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29040080/detection-of-giardia-intestinalis-in-water-samples-collected-from-natural-water-reservoirs-and-wells-in-northern-and-north-eastern-poland-using-lamp-real-time-pcr-and-nested-pcr
#14
Anna Lass, Beata Szostakowska, Krzysztof Korzeniewski, Panagiotis Karanis
Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal-oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (real-time PCR) and nested PCR...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/28979346/prevalence-and-risk-factors-of-human-intestinal-parasites-in-roudehen-tehran-province-iran
#15
Nasrin Hemmati, Elham Razmjou, Saeideh Hashemi-Hafshejani, Abbas Motevalian, Lameh Akhlaghi, Ahmad Reza Meamar
BACKGROUND: Intestinal parasitic infections are among the most common infections and health problems worldwide. Due to the lack of epidemiologic information of such infections, the prevalence of, and the risk factors for, enteric parasites were investigated in residents of Roudehen, Tehran Province, Iran. METHODS: In this cross-sectional study, 561 triple fecal samples were collected through a two-stage cluster-sampling protocol from Jun to Dec 2014. The samples were examined by formalin-ether concentration, culture, and with molecular methods...
July 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28959734/drug-free-approach-to-study-the-unusual-cell-cycle-of-giardia-intestinalis
#16
Kathleen Horlock-Roberts, Chase Reaume, Guillem Dayer, Christine Ouellet, Nicholas Cook, Janet Yee
Giardia intestinalis is a protozoan parasite that causes giardiasis, a form of severe and infectious diarrhea. Despite the importance of the cell cycle in the control of proliferation and differentiation during a giardia infection, it has been difficult to study this process due to the absence of a synchronization procedure that would not induce cellular damage resulting in artifacts. We utilized counterflow centrifugal elutriation (CCE), a size-based separation technique, to successfully obtain fractions of giardia cultures enriched in G1, S, and G2...
September 2017: MSphere
https://www.readbyqxmd.com/read/28924741/spectrum-of-infectious-diseases-among-newly-arrived-eritrean-refugees-in-switzerland-a-cross-sectional-study
#17
Afona Chernet, Andreas Neumayr, Christoph Hatz, Kerstin Kling, Véronique Sydow, Katharina Rentsch, Jürg Utzinger, Nicole Probst-Hensch, Hanspeter Marti, Beatrice Nickel, Niklaus D Labhardt
OBJECTIVES: Our study aimed at determining the prevalence of selected infectious diseases among recently arrived Eritrean refugees in Switzerland. METHODS: In this cross-sectional study, asymptomatic Eritrean migrants aged ≥16 years who arrived <24 months ago were recruited at refugee centres in Switzerland. Infectious disease screening included serology for HIV, hepatitis B and C, syphilis and schistosomiasis, polymerase chain reaction (PCR) for malaria, stool microscopy for helminths and intestinal protozoa and circulating cathodic antigen (CCA) testing in urine for schistosomiasis...
September 19, 2017: International Journal of Public Health
https://www.readbyqxmd.com/read/28884403/resonance-raman-studies-on-the-flavohemoglobin-of-the-protist-giardia-intestinalis-evidence-of-a-type-i-ii-peroxidase-like-heme-environment-and-roles-of-the-active-site-distal-residues
#18
Brian Lukaszewicz, Eliza McColl, Janet Yee, Steven Rafferty, Manon Couture
Flavohemoglobins are microbial enzymes that counter nitrosative stress, but the details of their underlying enzymatic activities and structure-function relationships are not completely understood. These enzymes have been identified in Gram-negative bacteria, certain fungi, and the parasitic protist Giardia intestinalis (gFlHb) which, despite lacking the ability to make heme, encodes several hemeproteins. To gain knowledge about the biophysical properties of the active site of gFlHb, we used resonance Raman spectroscopy to probe the wild-type protein and variants at globin domain positions E11, E7, and B10 on the distal, ligand-binding side of the heme...
October 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28822909/variation-in-basal-body-localisation-and-targeting-of-trypanosome-rp2-and-for20-proteins
#19
Jane Harmer, Xin Qi, Gabriella Toniolo, Aysha Patel, Hannah Shaw, Fiona E Benson, Michael L Ginger, Paul G McKean
TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies - on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function...
July 13, 2017: Protist
https://www.readbyqxmd.com/read/28793025/environmental-aspects-related-to-tuberculosis-and-intestinal-parasites-in-a-low-income-community-of-the-brazilian-amazon
#20
Biatriz Araújo Cardoso, Fabio de Oliveira Fonseca, Antonio Henrique Almeida de Moraes, Ana Caroline Guedes Souza Martins, Nissa Vilhena da Silva Oliveira, Luana Nepomuceno Gondim Costa Lima, George Alberto da Silva Dias, Maria Helena Féres Saad
We carried out a cross-sectional study from January to December 2015 on 1,425 inhabitants from a floating population in the Brazilian Amazon (Murinin district, Pará State) to describe the population-based prevalence of tuberculosis (TB) from 2011 to 2014, recent TB contacts (rCts) latently infected with Mycobacterium tuberculosis (LTBI) , the coverage of the local health network, socio-environmental factors, and frequency of intestinal parasitic infection (IPI). We found that the sanitary structure was inadequate, with latrines being shared with other rooms within the same accommodation; well water was the main source of water, and 48% of families had low incomes...
August 7, 2017: Revista do Instituto de Medicina Tropical de São Paulo
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