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miRNA,Hepatic fibrosis

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https://www.readbyqxmd.com/read/29426919/dietary-supplementation-with-hybrid-palm-oil-alters-liver-function-in-the-common-marmoset
#1
Flavia Spreafico, Rafael Carvalho Sales, Judit Gil-Zamorano, Priscylla da Costa Medeiros, Maria-Jesús Latasa, Monique Ribeiro Lima, Sergio Augusto Lopes de Souza, Roberto Martin-Hernández, Diego Gómez-Coronado, Eduardo Iglesias-Gutierrez, Diana C Mantilla-Escalante, Maria das Graças Tavares do Carmo, Alberto Dávalos
Hybrid palm oil, which contains higher levels of oleic acid and lower saturated fatty acids in comparison with African palm oil, has been proposed to be somehow equivalent to extra virgin olive oil. However, the biological effects of its consumption are poorly described. Here we have explored the effects of its overconsumption on lipid metabolism in a non-human primate model, the common marmoset. Dietary supplementation of marmoset with hyperlipidic diet containing hybrid palm oil for 3 months did not modify plasma lipids levels, but increased glucose levels as compared to the supplementation with African palm oil...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29393361/baicalin-inhibits-pdgf-bb-induced-hepatic-stellate-cell-proliferation-apoptosis-invasion-migration-and-activation-via-the-mir-3595-acsl4-axis
#2
Xiongjian Wu, Fachao Zhi, Weijian Lun, Qiliang Deng, Wendi Zhang
Hepatic fibrosis is a physiological response to liver injury that includes a range of cell types. The pathogenesis of hepatic fibrosis currently focuses on hepatic stellate cell (HSC) activation into muscle fiber cells and fibroblasts. Baicalin is a flavone glycoside. It is the glucuronide of baicalein, which is extracted from the dried roots of Scutellaria baicalensis Georgi. Previous work focused on the anti‑viral, ‑inflammatory and ‑tumor properties of baicalin. However, the potential anti‑fibrotic effects and mechanisms of baicalin are not known...
January 25, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29391222/role-of-micrornas-in-the-pathogenesis-and-treatment-of-progressive-liver-injury-in-nafld-and-liver-fibrosis
#3
Qiaozhu Su, Virender Kumar, Neetu Sud, Ram I Mahato
Non-alcoholic fatty liver disease (NAFLD) increases the risk of various liver injuries, ranging from simple steatosis to non-alcoholic steatohepatitis (NASH), fibrosis and cirrhosis, and ultimately hepatocellular carcinoma (HCC). Ample evidence has suggested that aberrant expression of microRNAs (miRNAs) is functionally involved in the activation of cellular stress, inflammation and fibrogenesis in hepatic cells, including hepatocytes, Kupffer and hepatic stellate cells (HSCs), at different pathological stages of NAFLD and liver fibrosis...
January 29, 2018: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29323718/effect-of-mir-182-on-hepatic-fibrosis-induced-by-schistosomiasis-japonica-by-targeting-foxo1-through-pi3k-akt-signaling-pathway
#4
Yu Huang, Xiangxue Fan, Ran Tao, Qiqing Song, Likui Wang, Hongyue Zhang, Hongyan Kong, Jiaquan Huang
OBJECTIVE: The study aimed to investigate the impact of miR-182 and FOXO1 on S. japonica-induced hepatic fibrosis. METHODS: Microarray analysis was performed to screen out differential expressed miRNAs and mRNAs. Rat hepatic fibrosis model and human hepatocellular cell line LX-2 were used to study the effect of miR-182 and FOXO1. qRT-PCR and western blot were used to detect the expression of miR-182, FOXO1 or other fibrosis markers. The targeting relationship between FOXO1 and miR-182 was verified by luciferase reporter assay...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29248466/leptin-suppresses-microrna-122-promoter-activity-by-phosphorylation-of-foxo1-in-hepatic-stellate-cell-contributing-to-leptin-promotion-of-mouse-liver-fibrosis
#5
Qing Cao, Xiaofei Zhu, Xuguang Zhai, Li Ji, Fangyun Cheng, Yiqi Zhu, Pengfei Yu, Yajun Zhou
Adipocytokine leptin promotes hepatic stellate cell (HSC) activation (a key step in liver fibrogenesis) and liver fibrosis. microRNA-122 (miR-122) is the most abundant liver-specific miRNA and was demonstrated to inhibit liver fibrosis and reduced HSC proliferation. Our previous study revealed that leptin reduced miR-122 level in HSCs. This study was aimed to investigate whether leptin affected miR-122 promoter and the underlying mechanisms in HSCs. Results showed that leptin inhibited miR-122 promoter activity...
December 14, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29236317/association-of-rno-mir-183-96-182-cluster-with-diethylnitrosamine-induced-liver-fibrosis-in-wistar-rats
#6
Rajeev Chandel, Roli Saxena, Ashim Das, Jyotdeep Kaur
Chronic liver injury due to various etiological factors including environmental carcinogens results in development of liver fibrosis. Numerous studies showed role of miRNAs in liver fibrosis. In the present study, we determined the rno-miR-183-96-182 cluster expression during hepatic fibrosis induced by diethylnitrosamine (DEN) treated Wistar rats and its association with plasma levels of circulating rno-miR-96, rno-miR-182, rno-miR-183, liver function test and lipid profile, aiming to identify their potential for histological stratification and early diagnosis of liver fibrosis...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29229992/mirna-21-ablation-protects-against-liver-injury-and-necroptosis-in-cholestasis
#7
Marta B Afonso, Pedro M Rodrigues, André L Simão, Maria M Gaspar, Tânia Carvalho, Paula Borralho, Jesús M Bañales, Rui E Castro, Cecília M P Rodrigues
Inhibition of microRNA-21 (miR-21) prevents necroptosis in the mouse pancreas. Necroptosis contributes to hepatic necro-inflammation in the common bile duct ligation (BDL) murine model. We aimed to evaluate the role of miR-21 in mediating deleterious processes associated with cholestasis. Mechanistic studies established a functional link between miR-21 and necroptosis through cyclin-dependent kinase 2-associated protein 1 (CDK2AP1). miR-21 expression increased in the liver of primary biliary cholangitis (PBC) patients and BDL wild-type (WT) mice at both 3 and 14 days...
December 11, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29196165/osteopontin-promotes-collagen-i-synthesis-in-hepatic-stellate-cells-by-mirna-129-5p-inhibition
#8
Yinghua Chen, Yitao Ou, Jiale Dong, Guizhi Yang, Zhi Zeng, Ying Liu, Bing Liu, Weidong Li, Xiaoshun He, Tian Lan
Activation of hepatic stellate cells (HSCs) is an essential event in the initiation and progression of liver fibrosis. HSCs are believed to be the major source of collagen-producing myofibroblasts in fibrotic livers. A key feature in the pathogenesis of liver fibrosis is fibrillar Collagen I (Col 1) deposition. Osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed in HSCs, could drive fibrogenesis by modulating the HSC pro-fibrogenic phenotype and Col 1 expression. Here, we aimed to investigate the molecular mechanism of OPN regulating the activation of HSCs...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29194684/global-micro-rna-expression-profiling-in-the-liver-biopsies-of-hepatitis-b-virus-infected-patients-suggests-specific-mirna-signatures-for-viral-persistence-and-hepatocellular-injury
#9
Avishek Kumar Singh, Sheetalnath Babasaheb Rooge, Aditi Varshney, Madavan Vasudevan, Ankit Bhardwaj, Senthil Kumar Venugopal, Nirupama Trehanpati, Manoj Kumar, Robert Geffers, Vijay Kumar, Shiv Kumar Sarin
Hepatitis B virus (HBV) can manipulate the miRNA regulatory networks in infected cells to create a permissive environment for viral replication, cellular injury, disease onset and its progression. The aim of the present study was to understand the miRNA networks and their target genes in the liver of hepatitis B patients involved in HBV replication, liver injury and liver fibrosis. We investigated differentially expressed miRNAs by microarray in the liver biopsy samples from different stages of HBV infection and liver disease [immune tolerant (IT; n= 8); acute viral hepatitis (AVH; n=8); no fibrosis (n=16); early (F1+F2) (n=19) or late fibrosis (F3+F4) (n=14) and healthy controls (n=7)]...
November 30, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29172295/evaluation-of-mir-224-mir-215-and-mir-143-as-serum-biomarkers-for-hcv-associated-hepatocellular-carcinoma
#10
Samah Mamdouh, Fatma Khorshed, Tarek Aboushousha, Hussam Hamdy, Ayman Diab, Mohamed Seleem, Mohamed Saber
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and α-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation...
November 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29170365/intrahepatic-microrna-profile-of-liver-transplant-recipients-with-hepatitis-c-virus-co-infected-with-human-immunodeficiency-virus
#11
Hisamitsu Miyaaki, Mitsuhisa Takatsuki, Tatsuki Ichikawa, Masaaki Hidaka, Akihiko Soyama, Hideki Ohdan, Yukihiro Inomata, Shinji Uemoto, Norihiro Kokudo, Kazuhiko Nakao, Susumu Eguchi
BACKGROUND In patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) co-infection, HIV can modulate HCV replication and immune response as well as accelerate liver fibrosis. The role of miRNA in HIV/HCV co-infection is not fully elucidated. The aim of this study was to examine the differential expression of miRNAs in the liver. MATERIAL AND METHODS Thirteen patients who had undergone a liver transplant (7 HCV-infected and 6 HIV/HCV-co-infected patients) were examined using a miRNA array containing 1347 human miRNAs...
November 24, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/29162670/pu-1-deficient-mice-are-resistant-to-thioacetamide-induced-hepatic-fibrosis-pu-1-finely-regulates-sirt1-expression-via-transcriptional-promotion-of-mir-34a-29c-in-hscs
#12
Qing Liu, Yongming Zhang, Songzhu Yang, Yanfang Wu, Jiantao Wang, Weiwei Yu, Yanguo Liu
PU.1 is a critical transcription factor involving in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1(+/-) mice, Sirt1 mRNA expression was not changed but Sirt1 protein was significantly increased, suggesting its promoting role in Sirt1 translation. We then isolated HSCs from WT and PU.1(+/-) mice, and the pcDNA-PU.1 expression vector was transfected into PU.1(+/-) HSCs. We checked the levels of miR-34a and miR-29c, two Sirt1-targeting miRNAs, and protein levels of PU...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29109031/pge2-induces-apoptosis-of-hepatic-stellate-cells-and-attenuates-liver-fibrosis-in-mice-by-downregulating-mir-23a-5p-and-mir-28a-5p
#13
R Brea, O Motiño, D Francés, C García-Monzón, J Vargas, M Fernández-Velasco, L Boscá, M Casado, P Martín-Sanz, N Agra
MicroRNAs (miRNAs), small noncoding RNAs modulating messenger RNA (mRNA) and protein expression, have emerged as key regulatory molecules in chronic liver diseases, whose end stage is hepatic fibrosis, a major global health burden. Pharmacological strategies for prevention or treatment of hepatic fibrosis are still limited, what makes it necessary to establish a better understanding of the molecular mechanisms underlying its pathogenesis. In this context, we have recently shown that cyclooxygenase-2 (COX-2) expression in hepatocytes restricts activation of hepatic stellate cells (HSCs), a pivotal event in the initiation and progression of hepatic fibrosis...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29100790/smurf2-an-e3-ubiquitin-ligase-interacts-with-pde4b-and-attenuates-liver-fibrosis-through-mir-132-mediated-ctgf-inhibition
#14
Yu Cai, Guanqun Huang, Lijie Ma, Ling Dong, She Chen, Xizhong Shen, Shuncai Zhang, Ruyi Xue, Deqiang Sun, Si Zhang
We previously reported that Smad ubiquitin regulatory factor 2 (Smurf2) activity was decreased in human fibrotic livers. Here, we overexpressed Smurf2 in livers of transgenic mice and observed inhibited collagen deposition and hepatic stellate cell activation in fibrotic model induced by carbon tetrachloride treatment or bile duct ligation. Hepatic Smurf2 overexpression also inhibited the production of connective tissue growth factor (CTGF), a central mediator of liver fibrosis. Using miRNA array and bioinformatics analyses, we identified miR-132 as a mediator of this inhibitory effect...
October 31, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29073595/epigenetically-regulated-microrna-9-5p-suppresses-the-activation-of-hepatic-stellate-cells-via-tgfbr1-and-tgfbr2
#15
Fujun Yu, BiCheng Chen, XuFei Fan, Guojun Li, Peihong Dong, Jianjian Zheng
BACKGROUND/AIMS: Recently, microRNAs (miRNAs) have been demonstrated to act as regulators of activation of hepatic stellate cells (HSCs). It is well known that the main profibrogenic inducer transforming growth factor-β1 (TGF-β1) contributes to HSC activation, which is a key event in liver fibrosis. Increasing studies show that miR-9-5p is down-regulated in liver fibrosis and restoration of miR-9-5p limits HSC activation. However, the role of miR-9-5p in TGF-β1-induced HSC activation is still not clear...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29054053/leptin-reduces-microrna-122-level-in-hepatic-stellate-cells-in-vitro-and-in-vivo
#16
Xuguang Zhai, Fangyun Cheng, Li Ji, Xiaofei Zhu, Qing Cao, Yali Zhang, Xin Jia, Qian Zhou, Wei Guan, Yajun Zhou
Obese patients, often accompanied by hyperleptinemia, are more likely to develop liver fibrosis. Leptin, an adipocyte-derived hormone, augments inflammatory in liver and promotes hepatic stellate cell (HSC) activation (a key step for liver fibrogenesis) and liver fibrosis. microRNA-122 (miR-122) is the most abundant liver-specific miRNA and can attenuate liver fibrosis. This study examined the effect of leptin on miR-122 level in HSCs in vivo and in vitro. Results demonstrated that leptin reduced the levels of both miR-122 (mature miR-122) and primary miR-122 (pri-miR-122)...
October 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29051972/mir-96-5p-prevents-hepatic-stellate-cell-activation-by-inhibiting-autophagy-via-atg7
#17
Kangkang Yu, Ning Li, Qi Cheng, Jianming Zheng, Mengqi Zhu, Suxia Bao, Mingquan Chen, Guangfeng Shi
Activation of hepatic stellate cell (HSC), which is the main source of extracellular matrix, plays a pivotal role in liver fibrogenesis. Autophagy of hepatic stellate cell has been recently implicated in liver fibrosis, but the regulation of hepatic stellate cell autophagy during this process remains poorly understood. Here, we first identified miR-96-5p as an aberrantly expressed miRNA in fibrotic liver tissues. Next, we transfected miR-96-5p mimic into human hepatic stellate cell line LX-2 and observed decreased protein and mRNA levels of α-SMA and Col1A1...
October 19, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29018509/circrna_0046367-prevents-hepatoxicity-of-lipid-peroxidation-an-inhibitory-role-against-hepatic-steatosis
#18
Xing-Ya Guo, Jian-Neng Chen, Fang Sun, Yu-Qin Wang, Qin Pan, Jian-Gao Fan
Hepatic steatosis reflects the miRNA-related pathological disorder with triglyceride accumulation and lipid peroxidation, which leads to nonalcoholic steatohepatitis, liver fibrosis/cirrhosis, and even hepatocellular carcinoma. Circular RNA (circRNA)/miRNA interaction reveals a novel layer of epigenetic regulation, yet the miRNA-targeting circRNA remains uncertain in hepatic steatosis. Here, we uncover circRNA_0046367 to be endogenous modulator of miR-34a that underlies hepatic steatosis. In contrast to its expression loss during the hepatocellular steatosis in vivo and in vitro, circRNA_0046367 normalization abolished miR-34a's inhibitory effect on peroxisome proliferator-activated receptor α (PPARα) via blocking the miRNA/mRNA interaction with miRNA response elements (MREs)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28953986/the-regulatory-role-of-il-6r-in-hepatitis-b-associated-fibrosis-and-cirrhosis
#19
Y Chen, S Yang, Y Peng, Z Yang
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay...
September 21, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28766848/microrna-30a-ameliorates-hepatic-fibrosis-by-inhibiting-beclin1-mediated-autophagy
#20
Jianliang Chen, Yue Yu, Shu Li, Yuting Liu, Shu Zhou, Shouji Cao, Jie Yin, Guoqiang Li
We explored the role of microRNA-30a (miR-30a) and the mechanism involved in hepatic fibrosis. MiR-30a overexpression was achieved by miR-30a mimics transfection in hepatic stellate cells (HSCs) (HSC-T6, LX-2), and miR-30a agomir (ago-miR-30a) treatment in mice. MiR-30a levels were measured using TaqMan miRNA assay system, and the localization of miR-30a was detected by fluorescence in situ hybridization (FISH). The interaction of miR-30a and Beclin1 was confirmed by dual-luciferase reporter assay. Autophagic flux was analysed using tandem mRFP-GFP-LC3 fluorescence microscopy, electron microscopy and Western blot of LC3-II/I ratio...
August 1, 2017: Journal of Cellular and Molecular Medicine
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