keyword
MENU ▼
Read by QxMD icon Read
search

miRNA,Hepatic fibrosis

keyword
https://www.readbyqxmd.com/read/29627726/generation-of-wae001-a-15-a-human-embryonic-stem-cell-line-with-mir-122-doxycycline-inducible-expression
#1
Yanli Liu, Feima Wu, Dong Liu, Ruzhi Wei, Nasir Abbas, Jianhong Xia, Dongwei Li, Haiyun Wang, Yuanqi Zhuang, Dongsheng Guo, Yan Chen, Yuhang Wu, Xinrong Ke, Jiawang Tao, Fan Yang, Keyu Lai, Xiaodong Shu, Yin-Xiong Li
MiR-122 is the most abundant miRNA in the human liver accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 is a valuable therapeutic target for liver diseases, including viral hepatitis, fibrosis, steatosis, and hepatocarcinoma. Here, we constructed a miR-122 doxycycline-inducible expression human embryonic stem cell line WAe001-A-15 using the piggyBac transposon system. The cell line retained its pluripotency, in vitro differentiation potential, normal morphology, and karyotype...
March 23, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29587268/microrna-30a-suppresses-the-activation-of-hepatic-stellate-cells-by-inhibiting-epithelial-to-mesenchymal-transition
#2
Jianjian Zheng, Wei Wang, Fujun Yu, Peihong Dong, Bicheng Chen, Meng-Tao Zhou
BACKGROUND/AIMS: The activation of hepatic stellate cells (HSCs) is considered as a pivotal event in liver fibrosis and epithelial-mesenchymal transition (EMT) process has been reported to be involved in HSC activation. It is known that microRNAs (miRNAs) play a pro-fibrotic or anti-fibrotic role in HSC activation. Recently, emerging studies show that miR-30a is down-regulated in human cancers and over-expression of miR-30a inhibits tumor growth and invasion via suppressing EMT process...
March 20, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29564929/hepatotoxicity-after-paracetamol-overdose-in-a-patient-with-cystic-fibrosis-despite-early-acetylcysteine-and-utility-of-microrna-to-predict-hepatotoxicity
#3
Anselm Wong, Benjamin Cheung, Charlotte Nejad, Michael Gantier, Andis Graudins
CASE DETAILS: A 19-year-old girl presented to the emergency department following overdose of 10 g of paracetamol on a background history of cystic fibrosis. Paracetamol concentration was below the nomogram line, but was treated with acetylcysteine seven hours post-overdose given her symptomatology. Nineteen hours following her overdose she developed hepatotoxicity, despite early initiation of acetylcysteine. She was discharged well six days post-ingestion. On presentation, delta miRNA-122-miR483 was 20 times that of control patients, however, alanine aminotransferase was normal...
March 22, 2018: Clinical Toxicology
https://www.readbyqxmd.com/read/29554131/down-regulation-of-microrna-203-3p-initiates-type-2-pathology-during-schistosome-infection-via-elevation-of-interleukin-33
#4
Xing He, Jun Xie, Yange Wang, Xiaobin Fan, Qin Su, Yue Sun, Nanhang Lei, Dongmei Zhang, Guangping Gao, Weiqing Pan
The type 2 immune response is the central mechanism of disease progression in schistosomiasis, but the signals that induce it after infection remain elusive. Aberrant microRNA (miRNA) expression is a hallmark of human diseases including schistosomiasis, and targeting the deregulated miRNA can mitigate disease outcomes. Here, we demonstrate that efficient and sustained elevation of miR-203-3p in liver tissues, using the highly hepatotropic recombinant adeno-associated virus serotype 8 (rAAV8), protects mice against lethal schistosome infection by alleviating hepatic fibrosis...
March 19, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29524604/altered-global-microrna-expression-in-hepatic-stellate-cells-lx-2-by-angiotensin-1-7-and-mirna-1914-5p-identification-as-regulator-of-pro-fibrogenic-elements-and-lipid-metabolism
#5
Brenda de Oliveira da Silva, Luciane Carla Alberici, Letícia Ferreira Ramos, Caio Mateus Silva, Marina Bonfogo da Silveira, Carlos R P Dechant, Scott L Friedman, Kumiko Koibuchi Sakane, Letícia Rocha Gonçalves, Karen C M Moraes
The development of new therapeutic strategies to control or reverse hepatic fibrosis requires thorough knowledge about its molecular and cellular basis. It is known that the heptapeptide angiotensin-(1-7) [ang-(1-7)] can reduce hepatic fibrosis and steatosis in vivo; therefore, it is important to uncover the mechanisms regulating its activity and cellular model of investigation. Ang-(1-7) is a peptide of the renin-angiotensin system (RAS), and here we investigated its modulatory effect on the expression pattern of microRNAs (miRNAs) in hepatic stellate cells (HSCs) LX-2, which transdifferentiate into fibrogenic and proliferative cells...
March 7, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29469324/-changes-of-liver-fibrosis-related-mirnas-induced-by-soluble-egg-antigen-of-schistosoma-japonicum
#6
Wang Huan, Lu Ya-Jing, Gao Yan-Ru, Wang Shu-Hong, Zhou Rui, Dong Hui-Fen
OBJECTIVE: To investigate the expression of miRNA associated with hepatic fibrosis induced by Schistosoma japonicum soluble egg antigen stimulation in mouse hepatocytes (AML12), so as to lay the foundation for clarifying the mechanism of schistosome infection leading to hepatic fibrosis. METHODS: The expressions of miR-122, miR-182, miR-23b, miR27b and KSRP in AML12 cells treated with SEA were measured by q-PCR. KSRP protein in cell lyses was measured by Western blotting...
March 8, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29426919/dietary-supplementation-with-hybrid-palm-oil-alters-liver-function-in-the-common-marmoset
#7
Flavia Spreafico, Rafael Carvalho Sales, Judit Gil-Zamorano, Priscylla da Costa Medeiros, Maria-Jesús Latasa, Monique Ribeiro Lima, Sergio Augusto Lopes de Souza, Roberto Martin-Hernández, Diego Gómez-Coronado, Eduardo Iglesias-Gutierrez, Diana C Mantilla-Escalante, Maria das Graças Tavares do Carmo, Alberto Dávalos
Hybrid palm oil, which contains higher levels of oleic acid and lower saturated fatty acids in comparison with African palm oil, has been proposed to be somehow equivalent to extra virgin olive oil. However, the biological effects of its consumption are poorly described. Here we have explored the effects of its overconsumption on lipid metabolism in a non-human primate model, the common marmoset. Dietary supplementation of marmoset with hyperlipidic diet containing hybrid palm oil for 3 months did not modify plasma lipids levels, but increased glucose levels as compared to the supplementation with African palm oil...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29393361/baicalin-inhibits-pdgf-bb-induced-hepatic-stellate-cell-proliferation-apoptosis-invasion-migration-and-activation-via-the-mir-3595-acsl4-axis
#8
Xiongjian Wu, Fachao Zhi, Weijian Lun, Qiliang Deng, Wendi Zhang
Hepatic fibrosis is a physiological response to liver injury that includes a range of cell types. The pathogenesis of hepatic fibrosis currently focuses on hepatic stellate cell (HSC) activation into muscle fiber cells and fibroblasts. Baicalin is a flavone glycoside. It is the glucuronide of baicalein, which is extracted from the dried roots of Scutellaria baicalensis Georgi. Previous work focused on the anti‑viral, ‑inflammatory and ‑tumor properties of baicalin. However, the potential anti‑fibrotic effects and mechanisms of baicalin are not known...
January 25, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29391222/micrornas-in-the-pathogenesis-and-treatment-of-progressive-liver-injury-in-nafld-and-liver-fibrosis
#9
Qiaozhu Su, Virender Kumar, Neetu Sud, Ram I Mahato
Non-alcoholic fatty liver disease (NAFLD) increases the risk of various liver injuries, ranging from simple steatosis to non-alcoholic steatohepatitis (NASH), fibrosis and cirrhosis, and ultimately hepatocellular carcinoma (HCC). Ample evidence has suggested that aberrant expression of microRNAs (miRNAs) is functionally involved in the activation of cellular stress, inflammation and fibrogenesis in hepatic cells, including hepatocytes, Kupffer and hepatic stellate cells (HSCs), at different pathological stages of NAFLD and liver fibrosis...
January 31, 2018: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29323718/effect-of-mir-182-on-hepatic-fibrosis-induced-by-schistosomiasis-japonica-by-targeting-foxo1-through-pi3k-akt-signaling-pathway
#10
Yu Huang, Xiangxue Fan, Ran Tao, Qiqing Song, Likui Wang, Hongyue Zhang, Hongyan Kong, Jiaquan Huang
OBJECTIVE: The study aimed to investigate the impact of miR-182 and FOXO1 on S. japonica-induced hepatic fibrosis. METHODS: Microarray analysis was performed to screen out differential expressed miRNAs and mRNAs. Rat hepatic fibrosis model and human hepatocellular cell line LX-2 were used to study the effect of miR-182 and FOXO1. qRT-PCR and western blot were used to detect the expression of miR-182, FOXO1 or other fibrosis markers. The targeting relationship between FOXO1 and miR-182 was verified by luciferase reporter assay...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29248466/leptin-suppresses-microrna-122-promoter-activity-by-phosphorylation-of-foxo1-in-hepatic-stellate-cell-contributing-to-leptin-promotion-of-mouse-liver-fibrosis
#11
Qing Cao, Xiaofei Zhu, Xuguang Zhai, Li Ji, Fangyun Cheng, Yiqi Zhu, Pengfei Yu, Yajun Zhou
Adipocytokine leptin promotes hepatic stellate cell (HSC) activation (a key step in liver fibrogenesis) and liver fibrosis. microRNA-122 (miR-122) is the most abundant liver-specific miRNA and was demonstrated to inhibit liver fibrosis and reduced HSC proliferation. Our previous study revealed that leptin reduced miR-122 level in HSCs. This study was aimed to investigate whether leptin affected miR-122 promoter and the underlying mechanisms in HSCs. Results showed that leptin inhibited miR-122 promoter activity...
January 15, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29236317/association-of-rno-mir-183-96-182-cluster-with-diethyinitrosamine-induced-liver-fibrosis-in-wistar-rats
#12
Rajeev Chandel, Roli Saxena, Ashim Das, Jyotdeep Kaur
Chronic liver injury due to various etiological factors including environmental carcinogens results in development of liver fibrosis. Numerous studies showed role of miRNAs in liver fibrosis. In the present study, we determined the rno-miR-183-96-182 cluster expression during hepatic fibrosis induced by diethylnitrosamine (DEN) treated Wistar rats and its association with plasma levels of circulating rno-miR-96, rno-miR-182, rno-miR-183, liver function test and lipid profile, aiming to identify their potential for histological stratification and early diagnosis of liver fibrosis...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29229992/mirna-21-ablation-protects-against-liver-injury-and-necroptosis-in-cholestasis
#13
Marta B Afonso, Pedro M Rodrigues, André L Simão, Maria M Gaspar, Tânia Carvalho, Paula Borralho, Jesús M Bañales, Rui E Castro, Cecília M P Rodrigues
Inhibition of microRNA-21 (miR-21) prevents necroptosis in the mouse pancreas. Necroptosis contributes to hepatic necro-inflammation in the common bile duct ligation (BDL) murine model. We aimed to evaluate the role of miR-21 in mediating deleterious processes associated with cholestasis. Mechanistic studies established a functional link between miR-21 and necroptosis through cyclin-dependent kinase 2-associated protein 1 (CDK2AP1). miR-21 expression increased in the liver of primary biliary cholangitis (PBC) patients and BDL wild-type (WT) mice at both 3 and 14 days...
December 11, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29196165/osteopontin-promotes-collagen-i-synthesis-in-hepatic-stellate-cells-by-mirna-129-5p-inhibition
#14
Yinghua Chen, Yitao Ou, Jiale Dong, Guizhi Yang, Zhi Zeng, Ying Liu, Bing Liu, Weidong Li, Xiaoshun He, Tian Lan
Activation of hepatic stellate cells (HSCs) is an essential event in the initiation and progression of liver fibrosis. HSCs are believed to be the major source of collagen-producing myofibroblasts in fibrotic livers. A key feature in the pathogenesis of liver fibrosis is fibrillar Collagen I (Col 1) deposition. Osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed in HSCs, could drive fibrogenesis by modulating the HSC pro-fibrogenic phenotype and Col 1 expression. Here, we aimed to investigate the molecular mechanism of OPN regulating the activation of HSCs...
January 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29194684/global-micro-rna-expression-profiling-in-the-liver-biopsies-of-hepatitis-b-virus-infected-patients-suggests-specific-mirna-signatures-for-viral-persistence-and-hepatocellular-injury
#15
Avishek Kumar Singh, Sheetalnath Babasaheb Rooge, Aditi Varshney, Madavan Vasudevan, Ankit Bhardwaj, Senthil Kumar Venugopal, Nirupama Trehanpati, Manoj Kumar, Robert Geffers, Vijay Kumar, Shiv Kumar Sarin
Hepatitis B virus (HBV) can manipulate the miRNA regulatory networks in infected cells to create a permissive environment for viral replication, cellular injury, disease onset and its progression. The aim of the present study was to understand the miRNA networks and their target genes in the liver of hepatitis B patients involved in HBV replication, liver injury and liver fibrosis. We investigated differentially expressed miRNAs by microarray in the liver biopsy samples from different stages of HBV infection and liver disease [immune tolerant (IT; n= 8); acute viral hepatitis (AVH; n=8); no fibrosis (n=16); early (F1+F2) (n=19) or late fibrosis (F3+F4) (n=14) and healthy controls (n=7)]...
November 30, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29172295/evaluation-of-mir-224-mir-215-and-mir-143-as-serum-biomarkers-for-hcv-associated-hepatocellular-carcinoma
#16
Samah Mamdouh, Fatma Khorshed, Tarek Aboushousha, Hussam Hamdy, Ayman Diab, Mohamed Seleem, Mohamed Saber
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and α-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation...
November 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29170365/intrahepatic-microrna-profile-of-liver-transplant-recipients-with-hepatitis-c-virus-co-infected-with-human-immunodeficiency-virus
#17
Hisamitsu Miyaaki, Mitsuhisa Takatsuki, Tatsuki Ichikawa, Masaaki Hidaka, Akihiko Soyama, Hideki Ohdan, Yukihiro Inomata, Shinji Uemoto, Norihiro Kokudo, Kazuhiko Nakao, Susumu Eguchi
BACKGROUND In patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) co-infection, HIV can modulate HCV replication and immune response as well as accelerate liver fibrosis. The role of miRNA in HIV/HCV co-infection is not fully elucidated. The aim of this study was to examine the differential expression of miRNAs in the liver. MATERIAL AND METHODS Thirteen patients who had undergone a liver transplant (7 HCV-infected and 6 HIV/HCV-co-infected patients) were examined using a miRNA array containing 1347 human miRNAs...
November 24, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/29162670/pu-1-deficient-mice-are-resistant-to-thioacetamide-induced-hepatic-fibrosis-pu-1-finely-regulates-sirt1-expression-via-transcriptional-promotion-of-mir-34a-and-mir-29c-in-hepatic-stellate-cells
#18
Qing Liu, Yongming Zhang, Songzhu Yang, Yanfang Wu, Jiantao Wang, Weiwei Yu, Yanguo Liu
PU box binding protein (PU.1) is a critical transcription factor involved in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1+/- mice, Sirt1 mRNA expression was not changed but Sirt1 protein was significantly increased, suggesting its promoting role in Sirt1 translation. We then isolated HSCs from wild-type (WT) and PU.1+/- mice, and the pcDNA-PU.1 expression vector was transfected into PU...
December 22, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29109031/pge-2-induces-apoptosis-of-hepatic-stellate-cells-and-attenuates-liver-fibrosis-in-mice-by-downregulating-mir-23a-5p-and-mir-28a-5p
#19
R Brea, O Motiño, D Francés, C García-Monzón, J Vargas, M Fernández-Velasco, L Boscá, M Casado, P Martín-Sanz, N Agra
MicroRNAs (miRNAs), small noncoding RNAs modulating messenger RNA (mRNA) and protein expression, have emerged as key regulatory molecules in chronic liver diseases, whose end stage is hepatic fibrosis, a major global health burden. Pharmacological strategies for prevention or treatment of hepatic fibrosis are still limited, what makes it necessary to establish a better understanding of the molecular mechanisms underlying its pathogenesis. In this context, we have recently shown that cyclooxygenase-2 (COX-2) expression in hepatocytes restricts activation of hepatic stellate cells (HSCs), a pivotal event in the initiation and progression of hepatic fibrosis...
February 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29100790/smurf2-an-e3-ubiquitin-ligase-interacts-with-pde4b-and-attenuates-liver-fibrosis-through-mir-132-mediated-ctgf-inhibition
#20
Yu Cai, Guanqun Huang, Lijie Ma, Ling Dong, She Chen, Xizhong Shen, Shuncai Zhang, Ruyi Xue, Deqiang Sun, Si Zhang
We previously reported that Smad ubiquitin regulatory factor 2 (Smurf2) activity was decreased in human fibrotic livers. Here, we overexpressed Smurf2 in livers of transgenic mice and observed inhibited collagen deposition and hepatic stellate cell activation in fibrotic model induced by carbon tetrachloride treatment or bile duct ligation. Hepatic Smurf2 overexpression also inhibited the production of connective tissue growth factor (CTGF), a central mediator of liver fibrosis. Using miRNA array and bioinformatics analyses, we identified miR-132 as a mediator of this inhibitory effect...
February 2018: Biochimica et Biophysica Acta
keyword
keyword
80185
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"