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https://www.readbyqxmd.com/read/29761411/detection-of-pathogenic-viruses-in-the-ambient-air-in-seoul-korea
#1
Tae-Hee Han, Sang-Hun Park, Ju-Young Chung, Hyo-Won Jeong, Jihun Jung, Jae-In Lee, Young-Ok Hwang, Il-Young Kim, Jip-Ho Lee, Kweon Jung
The possible transport of pathogenic microorganisms during Asian dust events could be an important concern for health workers; however, this is still uncertain owing to a lack of supporting evidence. The present study aimed to investigate the presence of pathogenic microorganisms in air samples collected during the Asian and non-Asian dust periods. Between March and September 2016, air samples were collected at three weather observation stations in Seoul using a high-volume air sampler. Multiplex PCR was performed using the Allplex™ respiratory and gastrointestinal panel assay kits to detect 46 microorganisms...
May 14, 2018: Food and Environmental Virology
https://www.readbyqxmd.com/read/29737264/what-are-hidden-facts-behind-intestinal-parasitic-infections-in-ilam-city
#2
Jahangir Abdi, Soghra Viesy, Zahra Rezaei
Intestinal parasitic infections are the one of the most common health problems in developing countries. A number of patients die annually due to complications caused by these parasites.Therefore, the aim of this study was to investigate the rate and type of parasitic infections, determine the factors affecting them in Ilam city and also provide strategies to prevent them. In this descriptive cross-sectional study conducted in one of the Ilam labs in 2016, 417 stool specimens were randomly collected. All specimens were examined using direct and ethanol formaldehyde...
May 8, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29587846/evaluation-of-two-dna-extraction-methods-for-the-pcr-based-detection-of-eukaryotic-enteric-pathogens-in-fecal-samples
#3
Estelle Menu, Charles Mary, Isabelle Toga, Didier Raoult, Stéphane Ranque, Fadi Bittar
OBJECTIVE: Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative PCR cycle threshold (Ct) values, the efficiency of two DNA extraction kits: the semi-automated EZ1® (Qiagen) and the manual QIAamp® DNA Stool Mini Kit (Qiagen), on six protozoa: Blastocystis spp., Cryptosporidium parvum/hominis, Cyclospora cayetanensis, Dientamoeba fragilis, Giardia intestinalis and Cystoisospora belli and one microsporidia: Enterocytozoon bieneusi...
March 27, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29581055/evaluation-of-the-roche-lightmix-%C3%A2-gastro-parasites-multiplex-pcr-assay-detecting-giardia-duodenalis-entamoeba-histolytica-cryptosporidia-dientamoeba-fragilis-and-blastocystis-hominis
#4
Johannes Friesen, Jörg Fuhrmann, Heike Kietzmann, Egbert Tannich, Michael Müller, Ralf Ignatius
OBJECTIVES: Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix® Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. METHODS: Stool specimens (n=1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen® enzyme immunoassays (EIAs) detecting G...
March 23, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29536712/-epidemic-status-of-human-key-parasitic-diseases-in-tongcheng-city-anhui-province
#5
Jiang Long-Zhi
OBJECTIVE: To understand the current status of key parasitic diseases in Tongcheng City, so as to provide an epidemiological reference for formulating the scientific planning for "13th Five-year" parasitic diseases prevention and control. METHODS: The villages of different levels in Tongcheng City were sampled to investigate the infection of parasitic diseases of residents aged three or more years. Soil-transmitted nemathelminth, intestinal protozoa and Enterobius vermicularis were investigated by the Kato-Katz technique (a fecal sample seized two), iodine smear method and cellophane tape method, respectively...
October 24, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29529848/prevalence-of-intestinal-parasites-and-associated-risk-factors-for-infection-among-rural-communities-of-chachoengsao-province-thailand
#6
Pisit Suntaravitun, Amornrat Dokmaikaw
Intestinal parasitic infections remain a major public health problem in many parts of Thailand, particularly in rural areas. This study aimed to determine the prevalence of intestinal parasitic infections and associated risk factors among the people living in Huai Sai sub-district, Bang Khla district, Chachoengsao Province, central Thailand. A cross-sectional survey was carried out from June 2017 to August 2017 which included a total of 224 participants. Stool samples were examined using a simple direct smear and formalin ethyl acetate concentration technique...
February 2018: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29522552/intestinal-parasite-infections-in-a-rural-community-of-rio-de-janeiro-brazil-prevalence-and-genetic-diversity-of-blastocystis-subtypes
#7
Carolina Valença Barbosa, Magali Muniz Barreto, Rosemary de Jesus Andrade, Fernando Sodré, Claudia Masini d'Avila-Levy, José Mauro Peralta, Ricardo Pereira Igreja, Heloisa Werneck de Macedo, Helena Lucia Carneiro Santos
BACKGROUND: Intestinal parasitic infections are considered a serious public health problem and widely distributed worldwide, mainly in urban and rural environments of tropical and subtropical countries. Globally, soil-transmitted helminths and protozoa are the most common intestinal parasites. Blastocystis sp. is a highly prevalent suspected pathogenic protozoan, and considered an unusual protist due to its significant genetic diversity and host plasticity. METHODOLOGY/MAIN FINDINGS: A total of 294 stool samples were collected from inhabitants of three rural valleys in Rio de Janeiro, Brazil...
2018: PloS One
https://www.readbyqxmd.com/read/29498738/blastocystis-hominis-transmission-by-non-potable-water-a-case-report-in-italy
#8
Maria Cristina Angelici, Chiara Nardis, Riccardo Scarpelli, Paola Ade
In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method...
March 2, 2018: New Microbiologica
https://www.readbyqxmd.com/read/29491555/genomic-analysis-of-blastocystis-hominis-isolates-in-patients-with-hiv-positive-using-locus-ssu-rdna
#9
Anahita Razavi Piranshahi, Mehdi Tavalla, Shahram Khademvatan
Blastocystis hominis ( B. hominis ) is a protozoan zoonosis which clinical signs of infection with this parasite has been reported to be more severe in patients with weakened immune systems than healthy controls. So, the aim of the study was to evaluate genomic analysis of B. hominis isolates obtained from patients with HIV-positive using locus SSU-rDNA. At first, 268 stool samples were randomly collected from patients with HIV-positive referred to health centers of Khuzestan province, southwest of Iran. Formol-ether and direct smear techniques were used for the detection of parasitic agents...
March 2018: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29469358/-survey-and-analysis-of-epidemic-status-of-principal-human-parasitosis-in-ecological-region-of-huaiyang-hills-of-henan-province-in-2015
#10
Zhang Ya-Lan, Zhu Yan-Kun, Chen Wei-Qi, Deng Yan, Lin Xi-Meng, Li Peng, Zhang Hong-Wei
OBJECTIVE: To understand the epidemic status of principal human parasitosis in the ecological region of Huaiyang hills of Henan Province. METHODS: According to the scheme of The 3rd National Survey of Principal Human Parasites made by National Institute of Parasitic Diseases, the survey was performed based on the ecological regions. The stratified cluster sampling was made combined with the economic and geographical conditions. The infections of intestinal helminths and protozoans in permanent residents were respectively detected by Kato-Kats technique and iodine solution...
July 24, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29455921/clinical-and-epidemiological-characteristics-of-intestinal-parasite-infection-by-blastocystis-hominis
#11
C Ocaña-Losada, J A Cuenca-Gómez, M T Cabezas-Fernández, J Vázquez-Villegas, M J Soriano-Pérez, I Cabeza-Barrera, J Salas-Coronas
OBJECTIVES: Blastocystis hominis (B. hominis) is one of the most common intestinal parasites isolated in humans. The parasite can cause gastrointestinal symptoms or, in most cases, remain asymptomatic. There are issues concerning the parasite's pathogenic character. The aim of this study was to analyse the clinical and epidemiological characteristics of the parasite infection by B. hominis, with or without other parasitic co-infections. PATIENTS AND METHODS: An observational retrospective study was conducted of B...
April 2018: Revista Clínica Española
https://www.readbyqxmd.com/read/29454366/occurrence-of-blastocystis-sp-and-pentatrichomonas-hominis-in-sheep-and-goats-in-china
#12
Wen Chao Li, Kai Wang, Youfang Gu
BACKGROUND: Global data regarding the molecular epidemiology of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats are sparse. China has one of the largest sheep and goat populations in the world. In this study we investigated the occurrence of Blastocystis sp. and P. hominis in domestic sheep and goats in China, and analyzed the genetic characterization of these two parasite species. METHODS: In total, we collected fresh fecal samples from 832 sheep and 781 goats located on seven and ten farms, respectively, in the central eastern region of China...
February 17, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29378644/prevalence-of-and-risk-factors-for-malaria-filariasis-and-intestinal-parasites-as-single-infections-or-co-infections-in-different-settlements-of-gabon-central-africa
#13
Noé Patrick M'bondoukwé, Eric Kendjo, Denise Patricia Mawili-Mboumba, Jeanne Vanessa Koumba Lengongo, Christelle Offouga Mbouoronde, Dieudonné Nkoghe, Fousseyni Touré, Marielle Karine Bouyou-Akotet
BACKGROUND: Malaria, filariasis, and intestinal parasitic infections (IPIs) are common and frequently overlap in developing countries. The prevalence and predictors of these infections were investigated in three different settlements (rural, semi-urban, and urban) of Gabon. METHODS: During cross-sectional surveys performed from September 2013 to June 2014, 451 individuals were interviewed. In addition, blood and stool samples were analysed for the presence of Plasmodium, filarial roundworm, intestinal protozoan, and helminth infections...
January 30, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29378626/prevalence-and-molecular-characterization-of-strongyloides-stercoralis-giardia-duodenalis-cryptosporidium-spp-and-blastocystis-spp-isolates-in-school-children-in-cubal-western-angola
#14
Elena Dacal, José M Saugar, Aida de Lucio, Marta Hernández-de-Mingo, Elena Robinson, Pamela C Köster, María L Aznar-Ruiz-de-Alegría, Mateu Espasa, Arlette Ninda, Javier Gandasegui, Elena Sulleiro, Milagros Moreno, Fernando Salvador, Israel Molina, Esperanza Rodríguez, David Carmena
BACKGROUND: Human infections by the gastrointestinal helminth Strongyloides stercoralis and the enteric protozoans Giardia duodenalis, Cryptosporidium spp. and Blastocystis spp. are not formally included in the list of 20 neglected tropical diseases prioritised by the World Health Organization. Although largely underdiagnosed and considered of lower public health relevance, these infections have been increasingly demonstrated to cause significant morbidity and even mortality globally, particularly among children living in resource-poor settings...
January 29, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29363443/a-cross-sectional-study-on-intestinal-parasitic-infections-in-children-in-suburban-public-primary-schools-saraburi-the-central-region-of-thailand
#15
Buravej Assavapongpaiboon, Uthaitip Bunkasem, Vivornpun Sanprasert, Surang Nuchprayoon
Intestinal parasitic infection rate among school-aged children in Thailand has been decreasing. However, certain intestinal parasites remain problematic in some regions. This cross-sectional study was conducted between February and September 2016 in three suburban government primary schools (KK, BR, and HK), Saraburi, Thailand. Stool was collected from 263 asymptomatic subjects (4-15 years old), using simple direct smear, formalin-ether concentration, Boeck and Drbohlav's Locke-Egg-Serum (LES) medium culture, and agar plate culture...
March 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29318125/prevalence-of-intestinal-parasites-in-food-handlers-of-bandar-abbas-southern-iran
#16
Mehrgan Heydari-Hengami, Yaghoob Hamedi, Majid Najafi-Asl, Khojasteh Sharifi-Sarasiabi
Background: Food handlers play a significant role in the dissemination and transmission of enteropathogenic microorganisms, including intestinal parasites. The aim of this study was to determine the prevalence of intestinal parasites in food handlers of Bandar Abbas, southern Iran. Methods: In this cross-sectional study, 800 stool samples were randomly collected in a period of 6 months from Jun to Nov 2015. The diagnosis was made on direct wet mount, formalin-ether concentration, Ziehl-Neelsen, and Trichrome stained slides...
January 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29260648/enteroparasites-in-preschool-children-on-the-pacific-region-of-nicaragua
#17
Carla Muñoz-Antoli, Mónica Gozalbo, Aleyda Pavón, Paloma Pérez, Rafael Toledo, Jose-Guillermo Esteban
The aim of this study was to determine for the first time the prevalence of enteroparasites in preschool children originating from the seven departments of the Pacific region in Nicaragua. One stool sample of each of 1,217 children, from 6 months to 5 years of age, was collected and personal data were recorded on delivery of the container. Samples fixed in 10% formalin were processed by a formol-acetate concentration and a modified Ziehl-Neelsen technique. The overall prevalence of enteroparasite infections was 68...
February 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29237648/parasitic-stool-testing-in-newly-arrived-refugees-in-calgary-alta
#18
Giselle DeVetten, Meghan Dirksen, Robert Weaver, Tanvir Turin Chowdhury, Michael William Aucoin
OBJECTIVE: To determine the prevalence of intestinal parasites and rates of stool testing compliance, as well as associated patient characteristics, among newly arrived refugees at the Mosaic Refugee Health Clinic in Calgary, Alta. DESIGN: Retrospective chart review. SETTING: Primary care clinic for refugee patients. PARTICIPANTS: A total of 1390 new refugee patients at the clinic from May 1, 2011, to June 30, 2013. MAIN OUTCOME MEASURES: Stool ova and parasite test completion and proportion of positive test results...
December 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29103267/intestinal-parasitic-infections-and-environmental-water-contamination-in-a-rural-village-of-northern-lao-pdr
#19
Alexis Ribas, Chloé Jollivet, Serge Morand, Boupha Thongmalayvong, Silaphet Somphavong, Chern-Chiang Siew, Pei-Jun Ting, Saipin Suputtamongkol, Viengsaene Saensombath, Surapol Sanguankiat, Boon-Huan Tan, Phimpha Paboriboune, Kongsap Akkhavong, Kittipong Chaisiri
A field survey studying intestinal parasites in humans and microbial pathogen contamination at environment was performed in a Laotian rural village to identify potential risks for disease outbreaks. A parasitological investigation was conducted in Ban Lak Sip village, Luang Prabang, Lao PDR involving fecal samples from 305 inhabitants as well as water samples taken from 3 sites of the local stream. Water analysis indicated the presence of several enteric pathogens, i.e., Aeromonas spp., Vibrio spp., E. coli H7, E...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29055284/multiple-sampling-and-saf-fixative-triple-faeces-testing-for-dysbiosis-and-pathogenic-infections-of-the-gastrointestinal-tract-case-report
#20
Johanna Mauss, Michelle Retz, Roger Dilts, Jeffrey Langland
The classic presentation of acute gastrointestinal (GI) parasitic infection including fever, diarrhea, nausea, and/or abdominal pain is not always a reliable measure. In these cases, parasitic infections require sensitive stool testing to be identified and treated effectively. The standard practice for stool analysis typically requires 3 separate stool specimens to be collected on 3 separate days to overcome the possibility of misdiagnosis due to intermittent shedding. Nonetheless, this practice is not often utilized due to very short periods of sample viability, which often leads to patient misdiagnosis...
October 21, 2017: Alternative Therapies in Health and Medicine
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