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secretory immunoglobulin a

Mariona Camps-Bossacoma, Francisco J Pérez-Cano, Àngels Franch, Margarida Castell
Background: A 10% cocoa-enriched diet influences immune system functionality including the prevention of the antibody response and the induction of lower immunoglobulin (Ig) concentrations. However, neither cocoa polyphenols nor cocoa fiber can totally explain these immunoregulatory properties. Objectives: This study aimed to establish the influence of cocoa theobromine in systemic and intestinal Ig concentrations and to determine the effect of cocoa or theobromine feeding on lymphoid tissue lymphocyte composition...
March 1, 2018: Journal of Nutrition
Tyler D R Vance, Laurie A Graham, Peter L Davies
Out of the dozen different ice-binding protein (IBP) structures known, the DUF3494 domain is the most widespread, having been passed many times between prokaryotic and eukaryotic microorganisms by horizontal gene transfer. This ~25-kDa β-solenoid domain with an adjacent parallel α-helix is most commonly associated with an N-terminal secretory signal peptide. However, examples of the DUF3494 domain preceded by tandem Bacterial Immunoglobulin-like (BIg) domains are sometimes found, though uncharacterized. Here we present one such protein (SfIBP_1) from the Antarctic bacterium Shewanella frigidimarina...
March 2, 2018: FEBS Journal
Joanna Orysiak, Konrad Witek, Jadwiga Malczewska-Lenczowska, Agnieszka Zembron-Lacny, Andrzej Pokrywka, Dariusz Sitkowski
The aim of this study was to determine the effects of 17 days of training during preparation for the Ice Hockey Under 18 World Championship of the Polish ice hockey national team on the mucosal immune function and monitor upper respiratory tract infection (URTI) incidence before, during and after the competition. Twelve male ice hockey players (age 17.7±0.5 years) were recruited for this study. The first saliva/blood collection took place at the beginning of the training camp (without training at the training camp), the second one was conducted on the 9th day of the training camp immediately after the intensification of training, and the third collection was carried out on the 13th day of training (4 days before leaving for the World Championship) in the tapering phase...
February 27, 2018: Journal of Strength and Conditioning Research
Sven Mathias, Simon Fischer, René Handrick, Jürgen Fieder, Patrick Schulz, Harald Bradl, Ingo Gorr, Martin Gamer, Kerstin Otte
With the advance of complex biological formats such as bispecific antibodies or fusion proteins, mammalian expression systems often show low performance. Described determining factors may be accumulation or haltering of heterologous proteins within the different cellular compartments disturbing transport or secretion. In case of the investigated bispecific antibody (bsAb)-producing Chinese hamster ovary (CHO) cell line neither impaired transcription nor decreased translation processes were identified and thus satisfactorily explained its low production capacity...
March 1, 2018: Journal of Biotechnology
Jiajun Zhou, Joseph Henriquez, Robert Crawford, Norbert Kaminski
Aryl hydrocarbon receptor (AHR) activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is well established at suppressing humoral immunity. Previous studies in mouse B cells revealed that decreased IgM production was due to a significant suppression in the mRNA levels of the immunoglobulin M components (IgH, IgJ and Igκ chains) and subsequent decrease in IgM synthesis. By contrast, the current study shows that activation of AHR in human B cells also results in a significant suppression of the number of IgM secreting cells, but this is not due to a decrease in the transcription or translation of IgH, IgJ and Igκ chains...
February 16, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Xiaohong Zhang, Tie Zhao, Tiebing Zeng, Ning Wu, Yongjian Xiao, Shuangquan Liu, Jian Yu, Chuanhao Jiang, Lin Gan, Meixia Deng, Xi Luo, Feijun Zhao
The present study aimed to evaluate the immune effect of intramuscular primary immunization by the nucleic acid vaccine pcDNA/glycerophosphodiester phosphodiesterase-interleukin-2 (pcDNA/Gpd-IL-2) and enhanced immunization 2 weeks later with the combination of mucosal adjuvant CpG-oligodeoxynucleotides (ODN) and Gpd-IL-2 recombinant protein on skin infection caused by Treponema pallidum (Tp) in New Zealand rabbits. At week 8 following immunization, MTT assay was used to detect spleen cell proliferation, while enzyme-linked immunosorbent assay was performed to detect the cytokine and secretory immunoglobulin A (SIgA) levels...
March 2018: Experimental and Therapeutic Medicine
Helen G Hanstock, Jason P Edwards, Ross Roberts, Neil P Walsh
Tear secretory immunoglobulin-A (SIgA) is a putative biomarker of common-cold risk with potential utility in non-invasive diagnostics. As SIgA secretion at the ocular surface is under strong autonomic control, we investigated the relationship between HR reactivity and tear SIgA responses to novel experiential stress. Thirty-two healthy participants undertook a 60-second zip-line ride to evoke acute stress and a seated-rest control trial in a randomised-crossover design. We recorded heart rate (HR) continuously and collected unstimulated tear samples 5-min-pre-, 2-min-post- and 20-min-post-stress/control...
February 7, 2018: Biological Psychology
Qiucheng Lei, Jingcheng Bi, Huanwei Chen, Feng Tian, Xuejin Gao, Ning Li, Xinying Wang
Parenteral nutrition (PN) is associated with increased infectious risks due to impaired intestinal immunity. Although glucagon-like peptide-2 (GLP-2) enhances the gut barrier function, it is uncertain whether it improves mucosal immunologic barrier function. We hypothesized that injecting the PN mouse model with GLP-2 improved innate and acquired immunity, and prevented bacterial translocation. Forty-eight hours after venous cannulation, male Institute of Cancer Research mice were randomly divided into 3 groups based on their diet: chow with saline (n = 10), PN (n = 9), or PN + GLP-2 (30 μg bid per mouse, n = 10) provided for 5 days...
January 2018: Nutrition Research
Yunjiang Yu, Ziling Yu, Peng Sun, Bigui Lin, Liangzhong Li, Zhengdong Wang, Ruixue Ma, Mingdeng Xiang, Hui Li, Shu Guo
This cross-sectional study investigated the association between air pollutant (AP) and respiratory health of 951 children residing near a municipal solid waste (MSW) landfill in Northern China. Results showed that students in non-exposure areas had significantly higher levels of lysozyme, secretory immunoglobulin A (SIgA), and better lung capacity than students in exposure areas (p < .05). Multiple regression model analysis indicated that lysozyme levels exhibited a consistent negative association with methane (CH4: β = -76...
February 5, 2018: Environmental Pollution
Patricia Burns, Sofia Oddi, Liliana Forzani, Eduardo Tabacman, Jorge Reinheimer, Gabriel Vinderola
OBJECTIVE: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. RESULTS: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility...
February 5, 2018: BMC Research Notes
Xia Hong, Yan-Yan Zhang, Wei Li, Yan-Ying Liu, Zhen Wang, Yan Chen, Yan Gao, Zhi-Peng Sun, Xin Peng, Jia-Zeng Su, Zhi-Gang Cai, Lei Zhang, Jing He, Li-Min Ren, Hong-Yu Yang, Zhan-Guo Li, Guang-Yan Yu
BACKGROUND: Immunoglobulin G4-related sialadenitis (IgG4-RS) is a newly recognized immune-mediated systemic disease. Despite its good response to steroid therapy, its treatment protocol is not standardized and the long-term outcome is controversial. The study was conducted to determine the short-term and long-term outcomes of IgG4-RS patients treated with glucocorticoids and steroid-sparing immunosuppressive agents, to analyze secretory function, serological and radiological changes in salivary glands and to assess the usefulness of serum IgG4 level as an indicator of disease activity...
January 30, 2018: Arthritis Research & Therapy
Annabel Kleinwort, Felix Lührs, Claus-Dieter Heidecke, Martin Lipp, Tobias Schulze
Introduction: Sphingosine-1-phosphate (S1P) regulates the migration of follicular B cells (B2 cells) and directs the positioning of Marginal zone B cells (MZ B cells) within the spleen. The function of S1P signalling in the third B cell lineage, B1 B cells, mainly present in the pleural and peritoneal cavity, has not yet been determined. Methods: S1P receptor expression was analysed in peritoneal B cells by real-time polymerase chain reaction (qPCR). The chemotactic response to S1P was studied in vitro. The role of S1P signalling was further explored in a s1p₄-/- mouse strain...
January 29, 2018: International Journal of Molecular Sciences
Svetla Todinova, Sashka Krumova, Avgustina Danailova, Violeta Petkova, Margarita Guenova, Georgi Mihaylov, Lidia Gartcheva, Stefka Germanova Taneva
The blood proteome has been studied extensively for identification of novel reliable disease biomarkers. In recent years, differential scanning calorimetry has emerged as a new tool for characterization of the thermodynamic properties of the major serum/plasma proteins and for the establishment of calorimetric markers for a variety of diseases. Here we applied calorimetry to monitor the effect of treatment of patients diagnosed with multiple myeloma and Waldenström's macroglobulinemia on the calorimetric profiles of patients' blood sera...
January 23, 2018: European Biophysics Journal: EBJ
Voraphoj Nilaratanakul, Jie Chen, Oanh Tran, Victoria K Baxter, Elizabeth M Troisi, Jane X Yeh, Diane E Griffin
Sindbis virus (SINV) infection of neurons in the brain and spinal cord in mice provides a model system for investigating recovery from encephalomyelitis and antibody-mediated clearance of virus from the central nervous system (CNS). To determine the roles of IgM and IgG in recovery, we compared the responses of immunoglobulin-deficient activation-induced adenosine deaminase (AID)-/-, secretory IgM (sIgM)-/- and AID-/- sIgM-/- double knock out (DKO) mice with wild-type (WT) C57BL/6 mice for disease, clearance of infectious virus and viral RNA from brain and spinal cord, antibody responses and B cell infiltration into the CNS...
January 10, 2018: Journal of Virology
Bradley W Richmond, Rui-Hong Du, Wei Han, John T Benjamin, Riet van der Meer, Linda Gleaves, Marshall Guo, Austin McKissack, Yongqin Zhang, Dong-Sheng Cheng, Vasiliy V Polosukhin, Timothy S Blackwell
Loss of secretory immunoglobulin A (SIgA) is common in the small airways of patients with chronic obstructive pulmonary disease (COPD) and may contribute to disease pathogenesis. Using mice that lack SIgA in the airways due to genetic deficiency of polymeric immunoglobulin receptor (pIgR-/- mice), we investigated the role of neutrophils in driving the fibrotic small airway wall remodeling and emphysema that develops spontaneously in these mice. By flow cytometry, we found an increase in the percentage of neutrophils among CD45+ cells in the lungs, as well as an increase in total neutrophils, in pIgR-/- mice compared to wild-type (WT) controls...
January 9, 2018: American Journal of Respiratory Cell and Molecular Biology
Anush Bakunts, Andrea Orsi, Milena Vitale, Angela Cattaneo, Federica Lari, Laura Tadè, Roberto Sitia, Andrea Raimondi, Angela Bachi, Eelco van Anken
Insufficient folding capacity of the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) to restore homeostasis. Yet, how the UPR achieves ER homeostatic readjustment is poorly investigated, since in most studies the ER-stress that is elicited cannot be overcome. Here we show that a proteostatic insult, achieved by persistent expression of the secretory heavy chain of immunoglobulin M (µs), is well-tolerated in HeLa cells. Upon µs expression, its levels temporarily eclipse those of the ER-chaperone BiP, leading to acute, full-geared UPR activation...
December 18, 2017: ELife
Y P Chen, C Wen, Y M Zhou
BACKGROUD: This study was conducted to investigate effects of dietary synbiotic supplementation as an alternative to antibiotic on growth performance, intestinal morphology, immunity and oxidative status of broilers. 1-day-old male chicks were allocated to 3 treatments, and given a basal diet free from antibiotic (Control group), and the basal diet supplemented with either chlortetracycline or synbiotic for 42 days, respectively. RESULTS: Compared with control group, the supplementation of synbiotic increased average daily gain and gain : feed ratio of broilers from 22 to 42 days and 1 to 42 days of age, and the relative weight of thymus and secretory immunoglobulin A level in the jejunum and ileum at 42 days of age, with the values of these parameters being similar to antibiotic group...
December 18, 2017: Journal of the Science of Food and Agriculture
Yongyao Yu, Yangzhou Liu, Huili Li, Shuai Dong, Qingchao Wang, Zhenyu Huang, Weiguang Kong, Xiaoting Zhang, Yongshen Xu, Xiaoyao Chen, Zhen Xu
The polymeric immunoglobulin receptor (pIgR) is an essential component of the mucosal immune system in jawed vertebrates including teleost fish, which mediate transepithelial transport of secretory immunoglobulins (sIgs) to protect organisms against environmental pathogens. In this study, we firstly cloned and identified the pIgR from dojo loach (Misgurnus anguillicaudatus). The full-length cDNA of Ma-pIgR was of 1145 bp, containing an open reading frame (ORF) of 1101 bp encoded a predicted protein of 336 amino acids...
December 14, 2017: Fish & Shellfish Immunology
Haifeng Sun, Jingcheng Bi, Qiucheng Lei, Xiao Wan, Tingting Jiang, Chao Wu, Xinying Wang
BACKGROUND AND AIMS: Partial enteral nutrition (PEN) protects parenteral nutrition (PN) induced gut mucosal immunity impairment. However, the gastrointestinal function of most patients with PN is too poor to tolerate full dosage of PEN and no guidelines recommend PEN dose. We aimed to identify an optimal PEN dose and to understand the protective mechanism. METHODS: Mice were assigned to groups with total parenteral nutrition (TPN), total enteral nutrition (TEN), or various degrees of PEN with PN...
December 14, 2017: International Journal of Surgery
Diana C Koester, David E Wildt, Morgan Maly, Pierre Comizzoli, Adrienne E Crosier
Approximately 80% of cheetahs living in typical zoological collections never reproduce. In more than 60% of breedings, the female is confirmed to ovulate, but parturition fails to occur. It is unknown if these non-pregnant intervals of elevated progesterone (deemed luteal phases) are conception failures or a pregnancy terminating in embryonic/fetal loss. There have been recent advances in metabolic profiling and proteome analyses in many species with mass spectrometry used to identify 'biomarkers' and mechanisms indicative of specific physiological states (including pregnancy)...
2017: PloS One
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