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secretory immunoglobulin a

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https://www.readbyqxmd.com/read/29143175/vectors-for-expression-of-signal-peptide-dependent-proteins-in-baculovirus-insect-cell-systems-and-their-application-to-expression-and-purification-of-the-high-affinity-immunoglobulin-gamma-fc-receptor-i-in-complex-with-its-gamma-chain
#1
Le T M Le, Jens R Nyengaard, Monika M Golas, Bjoern Sander
Integral membrane proteins play a central role in various cellular functions and are important therapeutic targets. However, technical challenges in the overexpression and purification of membrane proteins often represent a limiting factor for biochemical and structural studies. Here, we constructed a set of vectors, derivatives of MultiBac vectors that can be used to express proteins with a cleavable N-terminal signal peptide in insect cells. We propose these vectors for expression of type I membrane proteins and other secretory pathway proteins that require the signal recognition particle for translocation to the endoplasmic reticulum (ER)...
November 15, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29123984/transmembrane-thioredoxin-related-protein-tmx1-is-reversibly-oxidized-in-response-to-protein-accumulation-in-the-endoplasmic-reticulum
#2
Yoshiyuki Matsuo, Kiichi Hirota
Numerous secretory and membrane proteins undergo post-translational modifications in the endoplasmic reticulum (ER), and the formation of disulfide bonds is a modification that is critical for proper protein folding. The mammalian ER contains a large family of oxidoreductases that are considered to catalyze thiol/disulfide exchange and ensure the maintenance of a redox environment within the ER. Disruption of ER homeostasis causes an accumulation of misfolded and unfolded proteins, a condition termed ER stress...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29102516/colocalization-of-intracellular-specific-iga-iciga-with-influenza-virus-in-patients-nasopharyngeal-aspirate-cells
#3
Tuck-Weng Kok, Maurizio Costabile, Gregory A Tannock, Peng Li
Inhibition of viral replication by icIgA antibodies has only been observed with in vitro studies using epithelial cell lines in transwell cultures. This effect appears to involve an interaction between polymeric immunoglobulin A (pIgA) and viral particles within an intracellular compartment, since IgA is transported across polarized cells. Polyclonal guinea pig antisera against purified influenza A virus and mouse antisera prepared against Influenza A/H3N2 hemagglutinin (HA0) cleavage loop peptides, were used in confocal fluorescence microscopy to show specific staining of wild-type influenza H1N1 and H3N2 viruses in clinical specimens...
November 2, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/29093329/the-emerging-picture-of-mast-cell-activation-the-complex-regulatory-network-of-high-affinity-receptor-for-immunoglobulin-e-signaling
#4
Ryo Suzuki
It is now well known that immunoglobulin E (IgE) and mast cells (MCs) are important participants in allergic diseases. MCs contain electron-dense secretory granules which are filled with inflammatory mediators. The interaction of an allergen (antigen) with an antigen-specific IgE-bound high-affinity receptor for IgE (FcεRI) is an essential step in MC activation as well as subsequent downstream signaling events. What we know is that IgE and FcεRI activate a complex regulatory network (i.e., signaling molecules and messengers) that governs both the type of MC activation and the symptoms of allergic disease...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29077887/effects-of-dietary-supplementation-with-enzymatically-treated-artemisia-annua-on-growth-performance-intestinal-morphology-digestive-enzyme-activities-immunity-and-antioxidant-capacity-of-heat-stressed-broilers
#5
Z H Song, K Cheng, X C Zheng, H Ahmad, L L Zhang, T Wang
The gastrointestinal tract is considered as one of the main target organs affected by heat stress. Phytogenic feed additives containing phenolics and flavonoids can improve the resistance of broilers to heat stress. This study was conducted to investigate the effects of dietary supplementation with enzymatically treated Artemisia annua (EA) on growth performance, intestinal morphology, digestive enzyme activities, immunity and antioxidant capacity of broilers challenged with heat stress. One hundred and forty-four 21-day-old male Arbor Acres broilers were randomly distributed into 3 treatments: 1) non-challenged control (CON); 2) heat-stress-challenged control (HS); and 3) heat-stress-challenged group + 1 g EA/kg diet (HS-EA)...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29075899/immunoglobulin-a-and-liver-diseases
#6
REVIEW
Tatsuo Inamine, Bernd Schnabl
Immunoglobulin A (IgA) is a major immunoglobulin isotype in the gut and plays a role in maintenance of gut homeostasis. Secretory IgA (SIgA) has multiple functions in the gut, such as to regulate microbiota composition, to protect intestinal epithelium from pathogenic microorganisms, and to help for immune-system development. The liver is the front-line organ that receives gut-derived products through the portal vein, implying that the liver could be severely affected by a disrupted intestinal homeostasis. Indeed, some liver diseases like alcoholic liver disease are associated with an altered composition of gut microbiota and increased blood endotoxin levels...
October 26, 2017: Journal of Gastroenterology
https://www.readbyqxmd.com/read/29068402/oral-delivery-of-probiotics-expressing-dendritic-cell-targeting-peptide-fused-with-porcine-epidemic-diarrhea-virus-coe-antigen-a-promising-vaccine-strategy-against-pedv
#7
Xiaona Wang, Li Wang, Xuewei Huang, Sunting Ma, Meiling Yu, Wen Shi, Xinyuan Qiao, Lijie Tang, Yigang Xu, Yijing Li
Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, is the causative agent of porcine epidemic diarrhea (PED) that damages intestinal epithelial cells and results in severe diarrhea and dehydration in neonatal suckling pigs with up to 100% mortality. The oral vaccine route is reported as a promising approach for inducing protective immunity against PEDV invasion. Furthermore, dendritic cells (DCs), professional antigen-presenting cells, link humoral and cellular immune responses for homeostasis of the intestinal immune environment...
October 25, 2017: Viruses
https://www.readbyqxmd.com/read/29066918/asthma-with-bronchial-hypersecretion-expression-of-mucins-and-toll-like-receptors-in-sputum-and-blood
#8
Astrid Crespo-Lessmann, Eder Mateus, Montserrat Torrejón, Alicia Belda, Jordi Giner, Silvia Vidal, Oriol Sibila, Vicente Plaza
Asthma with bronchial hypersecretion is a type of asthma that is poorly studied. Its pathogenesis is not well understood, but is probably related to innate impaired immunity, particularly with toll-like receptors (TLRs) and secretory mucins (MUC). OBJECTIVES: 1) Define the clinical and inflammatory phenotype of asthma with bronchial hypersecretion of mucus. 2) Compare the type of mucin present in induced sputum (IS) of patients with and without bronchial hypersecretion. 3) Determine the expression of TLRs in IS and blood of asthmatics with and without bronchial hypersecretion...
2017: Journal of Asthma and Allergy
https://www.readbyqxmd.com/read/29052012/effect-of-nasal-antihistamine-on-secretory-iga-in-nasal-lavage-of-rats
#9
Remzi Doğan, Fadlullah Aksoy, Seda Sezen Goktas, Ilker Kocak, Yavuz Selim Yıldırım, Said Incir, Orhan Özturan
The humoral IgA is an immunoglobulin which plays a defensive role for organisms on mucosal surfaces. Today, intranasal antihistamines are effectively used in the treatment of allergic rhinitis. In our study, the effect of azelastine hydrochloride-a nasal antihistaminic-on humoral IgA of the nasal mucosa has been reviewed empirically. Twenty-four female Sprague-Dawley rats were included in our study. The rats were divided into three groups randomly. Group 1(azelastine hydrochloride): rats in this group had nasal azelastine hydrochloride (0...
October 19, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29032226/maternal-depressive-symptoms-linked-to-reduced-fecal-immunoglobulin-a-concentrations-in-infants
#10
Liane J Kang, Petya T Koleva, Catherine J Field, Gerald F Giesbrecht, Eytan Wine, Allan B Becker, Piushkumar J Mandhane, Stuart E Turvey, Padmaja Subbarao, Malcolm R Sears, James A Scott, Anita L Kozyrskyj
Secretory Immunoglobulin A (sIgA) plays a critical role to infant gut mucosal immunity. Delayed IgA production is associated with greater risk of allergic disease. Murine models of stressful events during pregnancy and infancy show alterations in gut immunity and microbial composition in offspring, but little is known about the stress-microbiome-immunity pathways in humans. We investigated differences in infant fecal sIgA concentrations according to the presence of maternal depressive symptoms during and after pregnancy...
October 12, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28984027/influence-of-a-preschool-preventive-dental-programme-on-caries-prevalence-oral-care-and-secretory-immunity-to-streptococcus-mutans-in-young-adults
#11
K L Aida, S de Paula Ramos, G F Seixas, A Bozza, R S Couto de Almeida, C C Dezan Garbelini
OBJECTIVE: To evaluate oral hygiene habits, decayed, missing and filled teeth (DMFT) and surfaces (DMFS), dental care, dietetic habits and anti-Streptococcus mutans salivary secretory Immunoglobulin A (SIgA) in young adults who attended a preventive programme during preschool age. MATERIAL AND METHODS: The study group (Baby Clinic) comprised 72 patients, aged 18-25 years, who had participated in the Baby Clinic preventive programme. The control group was age- and gender-matched...
October 6, 2017: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28979180/the-effect-of-different-workplace-nanoparticles-on-the-immune-systems-of-employees
#12
Natalja Kurjane, Tija Zvagule, Jelena Reste, Zanna Martinsone, Ilona Pavlovska, Inese Martinsone, Ivars Vanadzins
Currently, nanoparticles are widely present in the environment and are being used in various industrial technologies. Nanoparticles affect immune functions, causing different immune responses. The aim of the current study was to evaluate several cytokines, interleukin (IL)-1b, IL-6, IL-8, tumour necrosis factor-a (TNF-α), interferon-γ, adhesive molecule sICAM-1, macrophage inhibitory protein 1a (MIP1a) and secretory immunoglobulin A, in nasal lavage fluid and in the peripheral blood of healthy subjects exposed to workplace nanoparticles...
2017: Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology
https://www.readbyqxmd.com/read/28932905/serum-ige-and-igg4-against-muscle-larva-excretory-secretory-products-during-the-early-and-late-phases-of-human-trichinellosis
#13
Marcela A Calcagno, María A Forastiero, María P Saracino, Cecilia C Vila, Stella M Venturiello
In human trichinellosis, the relevance of the presence and persistence of specific serum IgE and IgG4 during the early and late phases of infection is still controversial.The aim of this work was to determine the percentage of human sera presenting IgE and IgG4 against Trichinella spiralis muscle larvae excretory-secretory products as well as their levels during the early and late phases of the infection. The antigen recognition pattern by serum total immunoglobulins (IgGAM), IgE, and IgG4 was assessed over time...
November 2017: Parasitology Research
https://www.readbyqxmd.com/read/28879521/fcrla-a-resident-endoplasmic-reticulum-protein-that-associates-with-multiple-immunoglobulin-isotypes-in-b-lineage-cells
#14
Tessa E Blackburn, Teresa Santiago, Peter D Burrows
FCRLA is homologous to receptors for the Fc portion of IgG (FcγR) and is located in the same region of human chromosome one, but has several unusual and unique features. It is a soluble resident ER protein retained in this organelle by unknown mechanisms involving the N-terminal domain, a disordered domain with three Cys residues in close proximity in the human protein. Unlike the FcγRs, FCRLA is not glycosylated and has no transmembrane region. FCRLA is included in this CTMI volume on IgM-binding proteins because it binds IgM in the ER, but quite surprisingly, given the isotype-restricted ligand specificity of the other FcRs, it also binds all other Ig isotypes so far tested, IgG and IgA...
September 7, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28866292/the-road-to-a-more-effective-influenza-vaccine-up-to-date-studies-and-future-prospects
#15
REVIEW
Kaori Sano, Akira Ainai, Tadaki Suzuki, Hideki Hasegawa
Influenza virus causes an acute respiratory infection in humans. Frequent point mutations in the influenza genome and occasional exchange of genetic segments between virus strains help the virus evade the pre-existing immunity, resulting in epidemics and pandemics. Although vaccination is the most effective intervention, mismatches between circulating viruses and vaccine strains reduce vaccine efficacy. Furthermore, current injectable vaccines induce IgG antibodies in serum (which limit progression of influenza symptoms) but not secretory IgA antibodies in the respiratory mucosa (which prevent virus infection efficiently)...
September 25, 2017: Vaccine
https://www.readbyqxmd.com/read/28858222/diurnal-changes-in-distribution-characteristics-of-salivary-cortisol-and-immunoglobulin-a-concentrations
#16
Hiromitsu Kobayashi, Chorong Song, Harumi Ikei, Bum-Jin Park, Takahide Kagawa, Yoshifumi Miyazaki
Salivary cortisol and secretory immunoglobulin A (S-IgA) are important biomarkers for environmental and public health research. The present study investigated the diurnal variations of these salivary biomarkers, with a focus on the change of distribution characteristics (i.e., skewness and kurtosis) of the concentrations. The participants in this study were 113 healthy young males. Salivary samples were collected in the morning (6:30-7:30 a.m.) and afternoon (1:00-4:00 p.m.). The skewness and kurtosis of salivary cortisol concentrations in afternoon samples (skewness = 1...
August 31, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28855300/an-alzheimer-associated-trem2-variant-occurs-at-the-adam-cleavage-site-and-affects-shedding-and-phagocytic-function
#17
Kai Schlepckow, Gernot Kleinberger, Akio Fukumori, Regina Feederle, Stefan F Lichtenthaler, Harald Steiner, Christian Haass
Sequence variations occurring in the gene encoding the triggering receptor expressed on myeloid cells 2 (TREM2) support an essential function of microglia and innate immunity in the pathogenesis of Alzheimer's disease (AD) and other neurodegenerative disorders. TREM2 matures within the secretory pathway, and its ectodomain is shed on the plasma membrane. Missense mutations in the immunoglobulin (Ig)-like domain such as p.T66M and p.Y38C retain TREM2 within the endoplasmic reticulum and reduce shedding as well as TREM2-dependent phagocytosis...
October 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28843478/chronic-alcohol-consumption-regulates-the-expression-of-poly-immunoglobulin-receptor-pigr-and-secretory-iga-in-the-gut
#18
María C López
The effect of ethanol (EtOH) on the gut immune system was analyzed using an experimental model previously described, where EtOH was provided ad libitum in the drinking water in a 20% w/v concentration for up to 12weeks. Dendritic cells, T cells and macrophages were analyzed in Peyer's patches and the small intestines using flow cytometry. Cytokine and immunoglobulin levels were analyzed in sera, feces, and homogenates from small and large intestines and lungs. Decreases in the proportion of T cells and alterations in dendritic cells and macrophages were observed after EtOH treatment...
October 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28835984/relapsing-remitting-type-1-diabetes
#19
Kayleigh M van Megen, Matthew P Spindler, Fleur M Keij, Ineke Bosch, Fleur Sprangers, Annet van Royen-Kerkhof, Tatjana Nikolic, Bart O Roep
AIMS/HYPOTHESIS: Type 1 diabetes is believed to be an autoimmune disease associated with irreversible loss of insulin secretory function that follows a chronic progressive course. However, it has been speculated that relapsing/remitting disease progression may occur in type 1 diabetes. METHODS: We report the case of an 18-year-old girl with Graves' disease, chronic inflammatory demyelinating polyneuropathy (CIDP) and multiple islet autoantibodies, presenting with relapsing/remitting hyperglycaemia...
November 2017: Diabetologia
https://www.readbyqxmd.com/read/28811748/salivary-diagnostic-markers-in-males-and-females-during-rest-and-exercise
#20
Kay Rutherfurd-Markwick, Carlene Starck, Deborah K Dulson, Ajmol Ali
BACKGROUND: Saliva is a useful diagnostic tool for analysis in sports, exercise and nutrition research, as collection is easy and non-invasive and it contains a large number of analytes affected by a range of physiological and pathological stressors and conditions. This study examined key salivary electrolytes and stress and immune markers in males and females at rest and during exercise. METHODS: Unstimulated whole saliva from 20 healthy, recreationally active participants (8 males and 12 females) was analysed for flow rate, osmolality, sodium (Na(+)), potassium (K(+)), chloride (Cl(-)), secretory immunoglobulin A (SIgA), α-amylase activity and cortisol during both rest and moderate intensity (70% peak power) cycling exercise in a randomised crossover design...
2017: Journal of the International Society of Sports Nutrition
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