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Justin Oh, Anahi Perlas, Johnny Lau, Rajiv Gandhi, Vincent W S Chan
STUDY OBJECTIVE: To compare the postoperative functional outcome and the total cost associated with outpatient vs inpatient care following complex hind-foot and ankle surgery. DESIGN: Retrospective, cohort study. SETTING: Tertiary care center. PATIENTS: Forty patients, American Society of Anesthesiologists 1-3, of either sex undergoing elective complex hind-foot and ankle surgery (fusion, osteotomy, or multiple ligament repair)...
December 2016: Journal of Clinical Anesthesia
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
November 9, 2016: Cell and Tissue Banking
Grisel Garcia Guillen, Veronica Anahi Zuñiga Ibarra, Maria Isabel Mendivil Palma, Bindu Krishnan, David Avellaneda Avellaneda, Sadasivan Shaji
Pulsed laser ablation in liquid (PLAL) is a green synthesis technique to obtain semiconductor nanomaterials in colloidal form. In this work, cadmium sulfide (CdS) nanoparticles were synthesized by pulsed laser ablation of a CdS target in different liquid media using 532 and 1064 nm outputs from a pulsed (10 ns, 10 Hz) Nd:YAG laser at different ablation fluence. Morphology, structure, crystalline phase, elemental composition, optical and luminescent properties of CdS nanomaterials were analyzed using Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy (XPS), UV-Visible absorption spectroscopy and fluorescence spectroscopy...
November 3, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Anahí Espíndola, Megan Ruffley, Megan L Smith, Bryan C Carstens, David C Tank, Jack Sullivan
Identifying units of biological diversity is a major goal of organismal biology. An increasing literature has focused on the importance of cryptic diversity, defined as the presence of deeply diverged lineages within a single species. While most discoveries of cryptic lineages proceed on a taxon-by-taxon basis, rapid assessments of biodiversity are needed to inform conservation policy and decision-making. Here, we introduce a predictive framework for phylogeography that allows rapidly identifying cryptic diversity...
October 26, 2016: Proceedings. Biological Sciences
Fekade Bruck Sime, Adam Johnson, Sarah Whalley, Anahi Santoyo-Castelazo, A Bruce Montgomery, Kathie Ann Walters, Jeffrey Lipman, William W Hope, Jason A Roberts
There has been a resurgence of interest in aerosolization of antibiotics for treatment of patients with severe pneumonia, caused by multi-drug resistant pathogens. A combination formulation of amikacin/fosfomycin is currently undergoing clinical testing although the exposure response-relationships of these drugs have not been fully characterized. The aim of this study was to describe the individual and combined antibacterial effects of simulated epithelial lining fluid exposures of aerosolized amikacin and fosfomycin against resistant clinical isolates of Pseudomonas aeruginosa (MICs 16 mg/L; 64 mg/L) and Klebsiella pneumoniae (MICs 2 mg/L; 64 mg/L) using the dynamic hollow-fiber infection model over 7-days...
October 24, 2016: Antimicrobial Agents and Chemotherapy
Anahi Guedes de Mello
Anthropology has increasingly questioned the hegemony of biomedical knowledge in ethical review processes of social research projects prevailing in Brazil, which was governed until 2012 by the Human Research Ethics Committee of each institution under the auspices of the National Research Ethics Commission (CONEP). This was mandated through Resolution No. 196/1996 prevailing in 2012 when this field research was conducted. The scope of this study is to recount and reflect upon the barriers to obtaining approval in 2012 for my master's research project from the Human Research Ethics Committee of the Federal University of Santa Catarina (CEP/UFSC) in Florianopolis...
October 2016: Ciência & Saúde Coletiva
Carlos L Hoyos, Silvana P Cajal, Marisa Juarez, Jorge D Marco, Anahí M Alberti D'Amato, Melina Cayo, Irma Torrejón, Rubén O Cimino, Patricio Diosque, Alejandro J Krolewiecki, Julio R Nasser, José F Gil
Background. Endemic areas of tegumentary leishmaniasis (TL) in Salta, Argentina, present some overlap zones with the geographical distribution of Chagas disease, with mixed infection cases being often detected. Objectives. The purpose of this study was to determine the magnitude of Leishmania sp. infection and potential associated risk factors, the serologic prevalence of T. cruzi, and the presence of T. cruzi-Leishmania sp. mixed infection in a region of the northwest of Argentina. Methods. Cross-sectional studies were conducted to detect TL prevalence and T...
2016: BioMed Research International
Denise Mathes, Johannes Weirather, Peter Nordbeck, Anahi-Paula Arias-Loza, Matthias Burkard, Christina Pachel, Thomas Kerkau, Niklas Beyersdorf, Stefan Frantz, Ulrich Hofmann
OBJECTIVE: The present study analyzed the effect of CD4(+) Forkhead box protein 3 negative (Foxp3(-)) T-cells and Foxp3(+) CD4(+) T-cells on infarct size in a mouse myocardial ischemia-reperfusion model. APPROACH AND RESULTS: We examined the infarct size as a fraction of the area-at-risk as primary study endpoint in mice after 30minutes of coronary ligation followed by 24hours of reperfusion. CD4(+) T-cell deficient MHC-II KO mice showed smaller histologically determined infarct size (34...
October 19, 2016: Journal of Molecular and Cellular Cardiology
Lucía N Biruk, Juan Moretton, Alicia Fabrizio de Iorio, Cristian Weigandt, Jimena Etcheverry, Javier Filippetto, Anahí Magdaleno
The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts...
October 17, 2016: Ecotoxicology and Environmental Safety
Catalina E Kopetz, Anahi Collado, Carl W Lejuez
The current research explores the idea that self-defeating behaviors represent means toward individuals' goals. In this quality, they may be automatically initiated upon goal activation without individual's voluntary intention and thus exemplify the long-held idea that the end justifies the means. To investigate this notion empirically we explored one of the most problematic self-defeating behavior: engagement in sex exchange for crack cocaine. This behavior is common among female drug users despite its well-known health and legal consequences...
December 2015: Motivation Science
Anahí Collado, Patrick S Johnson, Jennifer M Loya, Matthew W Johnson, Richard Yi
The study examined sexual delay discounting, or the devaluation of condom-protected sex in the face of delay, as a risk factor for sexually transmitted infection (STI) among college students. Participants (143 females, 117 males) completed the sexual delay discounting task (Johnson & Bruner, 2012) and questionnaires of risky sexual behavior, risk perception, and knowledge. Participants exhibited steeper sexual delay discounting (above and beyond general likelihood of having unprotected sex) when partners were viewed as more desirable or less likely to have a STI, with males demonstrating greater sexual delay discounting than females across most conditions...
October 3, 2016: Archives of Sexual Behavior
Thallita Pereira Queiroz, Rafael Scaf de Molon, Francisley Ávila Souza, Rogério Margonar, Anahi Herrera Aparecida Thomazini, Antônio Carlos Guastaldi, Eduardo Hochuli-Vieira
OBJECTIVE: We aimed to assess the surfaces of commercially pure titanium implants (cp Ti) with modified surfaces by laser beam (LS) with and without hydroxyapatite (HA) deposition, without (HAB) and with (HABT) thermal treatment. Furthermore, we have compared them with implants with surfaces modified by acid treatment (AS) and with machined surfaces (MS) utilizing histomorphometric and descriptive histologic analyses. MATERIAL AND METHODS: Surface topography characterization was analyzed by scanning electron microscopy (SEM), X-ray energy-dispersive spectroscopy (EDX), and surface roughness (Ra) before implant installation...
August 16, 2016: Clinical Oral Investigations
Kariem El-Boghdadly, Richelle Kruisselbrink, Vincent W S Chan, Anahi Perlas
No abstract text is available yet for this article.
September 2016: Anesthesiology
Rania Ghossoub, Louise Lindbæk, Anahi Molla-Herman, Alain Schmitt, Søren Tvorup Christensen, Alexandre Benmerah
In many vertebrate cell types, the proximal part of the primary cilium is positioned within an invagination of the plasma membrane known as the ciliary pocket. Recent evidence points to the conclusion that the ciliary pocket comprises a unique site for exocytosis and endocytosis of ciliary proteins, which regulates the spatiotemporal trafficking of receptors into and out of the cilium to control its sensory function. In this chapter, we provide methods based on electron microscopy, 3D reconstruction of fluorescence images as well as live cell imaging suitable for investigating processes associated with endocytosis at the ciliary pocket...
2016: Methods in Molecular Biology
Eliza Julia Pedraza-Brindis, Karina Sánchez-Reyes, Georgina Hernández-Flores, Alejandro Bravo-Cuellar, Luis Felipe Jave-Suárez, Adriana Aguilar-Lemarroy, Paulina Gómez-Lomelí, Brenda Anahí López-López, Pablo Cesar Ortiz-Lazareno
Patients with cervical cancer (CxCa) typically present an infiltrate of tumor-associated macrophages, which is associated with a poor prognosis. We found that CxCa cell lines (HeLa, SiHa, and C-33A) secreted factors involved in regulating tumor growth including IL-6, IL-4, PDGFAA, HGF, VEGF, ANG-2, and TGF-β3. We assessed the effects of culture supernatants from these cell lines on macrophages derived from the THP-1 cell line. Macrophages treated with culture supernatants from CxCa cells developed an M2-like phenotype with expression of CD163, low nitric oxide release, and high secretion of IL-6, PDGFAA, HGF, ANG-2, and VEGF...
July 16, 2016: Cellular Immunology
Anahi S Vieira, Lorena Narduche, Gabriel Martins, Igor A H F Schabib Péres, Namor P Zimmermann, Raquel S Juliano, Aiesca O Pellegrin, Walter Lilenbaum
Leptospiral infection is widespread in wildlife. In this context, wild ecosystems in tropical countries hold a vast biodiversity, including several species that may act as potential reservoirs of leptospires. The Pantanal biome presents highly favorable environmental conditions for the occurrence of leptospirosis, such as high temperatures, constant flooding, and high biodiversity. The purpose of this study was to detect wild animals as carriers of Leptospira sp. using direct methods (PCR and culture) in the Pantanal biome, Brazil...
November 2016: Acta Tropica
María Victoria Nieto Farias, Pamela Anahí Lendez, Maia Marin, Silvina Quintana, Lucía Martínez-Cuesta, María Carolina Ceriani, Guillermina Laura Dolcini
Bovine leukemia virus (BLV) infection is widespread mainly in dairy cattle and 5-10% of infected animals will die due to lymphosarcoma; most cattle remain asymptomatic but 30% develop persistent lymphocytosis (PL). BLV transmission depends on infected cell exchange and thus, proviral load is determinant. Understanding the mechanisms which govern the control of viral dissemination will be desirable for the design of effective therapeutic or preventive strategies for BLV. The development of high proviral load (HPL) or low proviral load (LPL) might be associated to genetic factors and humoral immune responses, however cellular responses are not fully described...
August 2016: Research in Veterinary Science
Anahi Perlas, Vincent W S Chan, Scott Beattie
BACKGROUND: This propensity score-matched cohort study evaluates the effect of anesthetic technique on a 30-day mortality after total hip or knee arthroplasty. METHODS: All patients who had hip or knee arthroplasty between January 1, 2003, and December 31, 2014, were evaluated. The principal exposure was spinal versus general anesthesia. The primary outcome was 30-day mortality. Secondary outcomes were (1) perioperative myocardial infarction; (2) a composite of major adverse cardiac events that includes cardiac arrest, myocardial infarction, or newly diagnosed arrhythmia; (3) pulmonary embolism; (4) major blood loss; (5) hospital length of stay; and (6) operating room procedure time...
October 2016: Anesthesiology
Ana Laura Colín-González, Marisol Maya-López, José Pedraza-Chaverrí, Syed F Ali, Anahí Chavarría, Abel Santamaría
No abstract text is available yet for this article.
July 7, 2016: Brain Research
Reinher Pimentel-Domínguez, Paola Moreno-Álvarez, Mathieu Hautefeuille, Anahí Chavarría, Juan Hernández-Cordero
Photothermal therapy has shown to be a promising technique for local treatment of tumors. However, the main challenge for this technique is the availability of localized heat sources to minimize thermal damage in the surrounding healthy tissue. In this work, we demonstrate the use of optical fiber microheaters for inducing thermal lesions in soft tissue. The proposed devices incorporate carbon nanotubes or gold nanolayers on the tips of optical fibers for enhanced photothermal effects and heating of ex vivo biological tissues...
April 1, 2016: Biomedical Optics Express
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