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https://www.readbyqxmd.com/read/28434066/toxicity-and-genotoxicity-of-three-antimicrobials-commonly-used-in-veterinary-medicine
#1
Anahí Magdaleno, Sofía Carusso, Juan Moretton
The toxicity of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) was tested on two green algal species: the international standard Pseudokirchneriella subcapitata and the native Argentine species Ankistrodesmus fusiformis. All three antibiotics inhibited the algal growth. The most sensitive species was P. subcapitata, for which the EC50 for CTC, OTC and ENF were 1.19 ± 0.53, 0.92 ± 0.30 and 5.18 ± 3.80 mg L(-1), respectively. The EC50 for A. fusiformis, were 3.23 ± 0...
April 22, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28387756/pore-forming-toxin-mediated-ion-dysregulation-leads-to-death-receptor-independent-necroptosis-of-lung-epithelial-cells-during-bacterial-pneumonia
#2
Norberto González-Juarbe, Kelley Margaret Bradley, Anukul Taranath Shenoy, Ryan Paul Gilley, Luis Felipe Reyes, Cecilia Anahí Hinojosa, Marcos Ignacio Restrepo, Peter Herman Dube, Molly Ann Bergman, Carlos Javier Orihuela
We report that pore-forming toxins (PFTs) induce respiratory epithelial cell necroptosis independently of death receptor signaling during bacterial pneumonia. Instead, necroptosis was activated as a result of ion dysregulation arising from membrane permeabilization. PFT-induced necroptosis required RIP1, RIP3 and MLKL, and could be induced in the absence or inhibition of TNFR1, TNFR2 and TLR4 signaling. We detected activated MLKL in the lungs from mice and nonhuman primates experiencing Serratia marcescens and Streptococcus pneumoniae pneumonia, respectively...
April 7, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28346980/ecological-intensification-to-mitigate-impacts-of-conventional-intensive-land-use-on-pollinators-and-pollination
#3
REVIEW
Anikó Kovács-Hostyánszki, Anahí Espíndola, Adam J Vanbergen, Josef Settele, Claire Kremen, Lynn V Dicks
Worldwide, human appropriation of ecosystems is disrupting plant-pollinator communities and pollination function through habitat conversion and landscape homogenisation. Conversion to agriculture is destroying and degrading semi-natural ecosystems while conventional land-use intensification (e.g. industrial management of large-scale monocultures with high chemical inputs) homogenises landscape structure and quality. Together, these anthropogenic processes reduce the connectivity of populations and erode floral and nesting resources to undermine pollinator abundance and diversity, and ultimately pollination services...
March 27, 2017: Ecology Letters
https://www.readbyqxmd.com/read/28342050/sewage-pollution-genotoxicity-assessment-and-phytoremediation-of-nutrients-excess-with-hydrocotyle-ranunculoides
#4
Gabriel Basílico, Anahí Magdaleno, Marta Paz, Juan Moretton, Ana Faggi, Laura de Cabo
The discharge of sewage effluents into low-order streams has negative effects on water quality. Macrophytes can be efficient in the treatment of this wastewater due to the removal of the main pollutants. The genotoxicity of sewage-polluted water discharging into La Choza stream was evaluated by testing with Allium cepa. Also, a phytoremediation assay with continuous recirculation of the residual water was conducted for 12 days. Three treatments were carried out. One treatment (Hr) was performed with a macrophyte (Hydrocotyle ranunculoides), and two treatments were conducted without macrophytes: with lighting (Ai) and without lighting (Ao)...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28337836/plant-transcription-factors-from-the-homeodomain-leucine-zipper-family-i-role-in-development-and-stress-responses
#5
REVIEW
María Florencia Perotti, Pamela Anahí Ribone, Raquel Lía Chan
In front of stressful conditions plants display adaptation mechanisms leading to changes in their morphology, physiology, development and molecular composition. Transcription factors (TFs) play crucial roles in these complex adaptation processes. This work is focused in the homeodomain-leucine zipper I (HD-Zip I) family of TFs, unique to plants. First discovered in 1991, they were identified and isolated from monocotyledonous and dicotyledonous plants showing high structural similarity and diversified functions...
March 24, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28290283/ace-inhibitory-peptides-from-bovine-caseins-released-with-peptidases-from-maclura-pomifera-latex
#6
María Alicia Corrons, Constanza Silvina Liggieri, Sebastián Alejandro Trejo, Mariela Anahí Bruno
In work reported here, a proteolytic extract prepared from Maclura pomifera latex was employed to hydrolyze bovine caseins. Densitograms of Tricine-sodium-dodecyl-sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) indicated that the caseins were considerably degraded after a 10-min reaction. The degree of hydrolysis determined by the 2,4,6-trinitrobenzenesulfonic-acid method was 17.1±0.7% after 180min of digestion. The concentration of small peptides increased with hydrolysis time, and analysis by reverse-phase high-performance liquid chromatography (RP HPLC) and mass spectrometry, revealed a virtually unchanged peptide profile...
March 2017: Food Research International
https://www.readbyqxmd.com/read/28273076/different-genotypes-of-trypanosoma-cruzi-produce-distinctive-placental-environment-genetic-response-in-chronic-experimental-infection
#7
Natalia Anahí Juiz, María Elisa Solana, Gonzalo Raúl Acevedo, Alejandro Francisco Benatar, Juan Carlos Ramirez, Priscilla Almeida da Costa, Andrea Mara Macedo, Silvia Andrea Longhi, Alejandro G Schijman
Congenital infection of Trypanosoma cruzi allows transmission of this parasite through generations. Despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/6J mice comparing placentas from uninfected animals and from animals infected with two different T. cruzi strains: K98, a clone of the non-lethal myotropic CA-I strain (TcI), and VD (TcVI), isolated from a human case of congenital infection...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28272292/the-erector-spinae-plane-block-provides-visceral-abdominal-analgesia-in-bariatric-surgery-a-report-of-3-cases
#8
Ki Jinn Chin, Laith Malhas, Anahi Perlas
Postoperative pain after bariatric surgery can be significant and yet difficult to manage. These patients frequently have associated obstructive sleep apnea and are at risk of respiratory depression with opioid analgesia. Abdominal wall blocks such as the subcostal transversus abdominis plane block are not of significant benefit, probably in part because they provide only somatic analgesia. The ultrasound-guided erector spinae plane (ESP) block is a recently described regional anesthetic technique for providing thoracic analgesia when performed at the level of the T5 transverse process...
May 2017: Regional Anesthesia and Pain Medicine
https://www.readbyqxmd.com/read/28257502/treatment-of-ruptured-intracranial-aneurysms-yesterday-and-now
#9
Alexander Hammer, Anahi Steiner, Ghassan Kerry, Gholamreza Ranaie, Ingrid Baer, Christian M Hammer, Stefan Kunze, Hans-Herbert Steiner
OBJECTIVE: This prospective study is designed to detect changes in the treatment of ruptured intracranial aneurysms over a period of 17 years. METHODS: We compared 361 treated cases of aneurysm occlusion after subarachnoid hemorrhage from 1997 to 2003 with 281 cases from 2006 to 2014. Specialists of neuroradiology and vascular neurosurgery decided over the modality assignment. We established a prospective data acquisition in both groups to detect significant differences within a follow-up time of one year...
2017: PloS One
https://www.readbyqxmd.com/read/28256443/cat-scratch-disease-during-etanercept-therapy-in-a-rheumatoid-arthritis-patient
#10
Alberto O Orden, Norma N Nardi, Alicia B Vilaseca, Ana C Colombini, Nora G Barrios, Anahí Vijnovich Barón
Cat scratch disease (CSD) is an infectious disorder caused by Bartonella henselae and characterized by fever and granulomatous lymphadenopathy. Immunosuppression is a risk factor for the development of atypical forms of the disease. We report the case of a 52-year-old woman who presented with fever and bilateral inguinal lymph node enlargement. She did not have apparent contact with animals. The patient was receiving etanercept therapy for rheumatoid arthritis. Lymph node biopsy demonstrated granulomatous lymphadenitis...
February 27, 2017: Reumatología Clinica
https://www.readbyqxmd.com/read/28193419/chemical-stability-of-astaxanthin-integrated-into-a-food-matrix-effects-of-food-processing-and-methods-for-preservation
#11
REVIEW
Alejandra Anahí Martínez-Delgado, Sanghamitra Khandual, Socorro Josefina Villanueva-Rodríguez
Astaxanthin is a carotenoid pigment found in numerous organisms ranging from bacteria to algae, yeasts, plants, crustaceans and fish such as salmon. Technological importance of this pigment emerged from various studies demonstrating that it is a powerful antioxidant, even with higher activity than alpha-tocopherol and other carotenoids. It has been included in various pharmaceutical products because of several beneficial properties. By its nature, astaxanthin is susceptible to degradation and can undergo chemical changes during food processing...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28107777/the-dc1-domain-protein-vacuoleless-gametophytes-is-essential-for-development-of-female-and-male-gametophytes-in-arabidopsis
#12
Sebastián D'Ippólito, Leonardo Agustín Arias, Claudia Anahí Casalongué, Gabriela Carolina Pagnussat, Diego Fernando Fiol
In this work we identified VACUOLELESS GAMETOPHYTES (VLG) as a DC1 domain-containing protein present in the endomembrane system and essential for development of both female and male gametophytes. VLG was originally annotated as a gene coding for a protein of unknown function containing DC1 domains. DC1 domains are cysteine- and histidine-rich zinc finger domains found exclusively in the plant kingdom that have been named on the basis of similarity with the C1 domain present in protein kinase C (PKC). In Arabidopsis, both male and female gametophytes are characterized by the formation of a large vacuole early in development; this is absent in vlg mutant plants...
January 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28094482/real-time-ultrasound-guided-spinal-anesthesia-in-patients-with-predicted-difficult-anatomy
#13
Hesham Elsharkawy, Ankit Maheshwari, Rovnat Babazade, Anahi Perlas, Sherif Zaky, Loran M Soliman
BACKGROUND: There are limited reports of lumbar neuraxial blocks using real-time ultrasound in patients with predicted difficulties. We compared the number of attempts to perform spinal anesthesia using real time ultrasound guidance versus landmark technique in patients meeting predefined criteria for difficult spinal anesthesia. We also compared procedure time, block success, patient satisfaction and difficulty scores between groups. METHODS: Following institutional review board approval patients scheduled for total hip or knee arthroplasty with expected difficulty to perform spinal anesthesia were included...
January 17, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/28045758/a-virtual-reality-simulation-model-of-spinal-ultrasound-role-in-teaching-spinal-sonoanatomy
#14
Reva Ramlogan, Ahtsham U Niazi, Rongyu Jin, James Johnson, Vincent W Chan, Anahi Perlas
BACKGROUND AND OBJECTIVES: Ultrasound assessment of the lumbar spine improves the success of spinal and epidural anesthesia, especially for patients with underlying difficult anatomy. To assist with the teaching and learning of ultrasound-guided neuraxial anesthesia, we have created an online interactive educational model (http://www.usra.ca/vspine.php and http://pie.med.utoronto.ca/vspine). The aim of the current study was to determine whether the virtual spine model improved the knowledge of neuraxial anatomy and sonoanatomy...
March 2017: Regional Anesthesia and Pain Medicine
https://www.readbyqxmd.com/read/28040421/anti-mda5-dermatomyositis-mimicking-psoriatic-arthritis
#15
Iván Cabezas-Rodríguez, Isla Morante-Bolado, Anahy Brandy-García, Rubén Queiro-Silva, Lourdes Mozo, Francisco Javier Ballina-García
Dermatomyositis causes inflammation and damage of muscle and skin, and sometimes involves internal organs, especially lung parenchyma. Patients with dermatomyositis still represent a diagnostic challenge because of the rarity of this disease and the lack of specificity of some of its cutaneous manifestations. Herein, we describe the case of a patient with dermatomyositis, initially diagnosed as psoriatic arthritis, in which the performance of anti-melanoma differentiation-associated gene 5 (MDA5) antibodies was decisive to establish a definitive diagnosis...
December 28, 2016: Reumatología Clinica
https://www.readbyqxmd.com/read/27997497/safety-and-subepineural-injections-a-reply-to-dr-bigeleisen
#16
LETTER
Anthony Short, Vincent W S Chan, Anahi Perlas
No abstract text is available yet for this article.
January 2017: Regional Anesthesia and Pain Medicine
https://www.readbyqxmd.com/read/27939811/integrating-knowledge-exchange-and-the-assessment-of-dryland-management-alternatives-a-learning-centered-participatory-approach
#17
Susana Bautista, Joan Llovet, Anahí Ocampo-Melgar, Alberto Vilagrosa, Ángeles G Mayor, Cristina Murias, V Ramón Vallejo, Barron J Orr
The adoption of sustainable land management strategies and practices that respond to current climate and human pressures requires both assessment tools that can lead to better informed decision-making and effective knowledge-exchange mechanisms that facilitate new learning and behavior change. We propose a learning-centered participatory approach that links land management assessment and knowledge exchange and integrates science-based data and stakeholder perspectives on both biophysical and socio-economic attributes...
December 8, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27939775/potential-of-an-outranking-multi-criteria-approach-to-support-the-participatory-assessment-of-land-management-actions
#18
Anahí Ocampo-Melgar, Susana Bautista, J Edward deSteiguer, Barron J Orr
We evaluated the potential of an outranking Multi-Criteria Decision Analysis approach for assisting in the participatory assessment of dryland management actions implemented in the San Simon watershed, in southeastern Arizona, USA. We compared an outranking-facilitated assessment of actions with a simple and direct (baseline) ranking of the same actions by the participating stakeholders in terms of: 1) internal homogeneity of each assessment approach, (2) similarity of individual assessments between methods, and (3) effects of the use of implicit/explicit assessment criteria...
December 7, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27871521/functional-outcome-and-cost-effectiveness-of-outpatient-vs-inpatient-care-for-complex-hind-foot-and-ankle-surgery-a-retrospective-cohort-study
#19
Justin Oh, Anahi Perlas, Johnny Lau, Rajiv Gandhi, Vincent W S Chan
STUDY OBJECTIVE: To compare the postoperative functional outcome and the total cost associated with outpatient vs inpatient care following complex hind-foot and ankle surgery. DESIGN: Retrospective, cohort study. SETTING: Tertiary care center. PATIENTS: Forty patients, American Society of Anesthesiologists 1-3, of either sex undergoing elective complex hind-foot and ankle surgery (fusion, osteotomy, or multiple ligament repair)...
December 2016: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/27830445/tissue-engineering-for-neurodegenerative-diseases-using-human-amniotic-membrane-and-umbilical-cord
#20
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
March 2017: Cell and Tissue Banking
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