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https://www.readbyqxmd.com/read/28774657/prevalence-of-rheumatic-diseases-in-adult-population-in-spain-episer-2016-study-aims-and-methodology
#1
Daniel Seoane-Mato, Carlos Sánchez-Piedra, Lucía Silva-Fernández, Francisca Sivera, Francisco J Blanco, Fernando Pérez Ruiz, Antonio Juan-Mas, José M Pego-Reigosa, Javier Narváez, Neus Quilis Martí, Raúl Cortés Verdú, Fred Antón-Pagés, Víctor Quevedo Vila, Gustavo Añez Sturchio, Fermín Medina Varo, María Del Mar Ruiz Tudela, Antonio Romero Pérez, Javier Ballina, Anahy Brandy García, Dolores Fábregas Canales, Teresa Font Gayá, Carolina Bordoy Ferrer, Beatriz González Álvarez, Laura Casas Hernández, Fátima Álvarez Reyes, Mónica Delgado Sánchez, Cristina Martínez Dubois, Simón Ángel Sánchez-Fernández, Luisa Marena Rojas Vargas, Paula Virginia García Morales, Alejandro Olivé, Paula Rubio Muñoz, Marta Larrosa, Noemí Navarro Ricos, Eduard Graell Martín, Eugenio Chamizo, Lara Chaves Chaparro, Sara Rojas Herrera, Jordi Pons Dolset, Miguel Ángel Polo Ostariz, Susana Ruiz-Alejos Garrido, Cristina Macía Villa, Ana Cruz Valenciano, María Luisa González Gómez, Mercedes Morcillo Valle, Deseada Palma Sánchez, María José Moreno Martínez, Marta Mayor González, Joana Atxotegi Sáenz de Buruaga, Irati Urionagüena Onaindia, Boris Anthony Blanco Cáceres, Federico Díaz-González, Sagrario Bustabad
AIMS: To describe the methodology of the EPISER 2016 (study of the prevalence of rheumatic diseases in adult population in Spain), as well its strengths and limitations. The aim of this study is to estimate the prevalence of rheumatoid arthritis (RA), psoriatic arthritis (PsA), ankylosing spondylitis (AS), systemic lupus erythematosus (SLE), Sjögren's syndrome (SS), osteoarthritis (knee, hip, hands, and cervical and lumbar spine), fibromyalgia, gout and clinical osteoporotic fracture...
July 31, 2017: Reumatología Clinica
https://www.readbyqxmd.com/read/28706090/improving-site-selection-in-clinical-studies-a-standardised-objective-multistep-method-and-first-experience-results
#2
Anahí Hurtado-Chong, Alexander Joeris, Denise Hess, Michael Blauth
INTRODUCTION: A considerable number of clinical studies experience delays, which result in increased duration and costs. In multicentre studies, patient recruitment is among the leading causes of delays. Poor site selection can result in low recruitment and bad data quality. Site selection is therefore crucial for study quality and completion, but currently no specific guidelines are available. MATERIAL AND METHODS: Selection of sites adequate to participate in a prospective multicentre cohort study was performed through an open call using a newly developed objective multistep approach...
July 12, 2017: BMJ Open
https://www.readbyqxmd.com/read/28706089/evaluation-of-the-geriatric-co-management-for-patients-with-fragility-fractures-of-the-proximal-femur-geriatric-fracture-centre-gfc-concept-protocol-for-a-prospective-multicentre-cohort-study
#3
Alexander Joeris, Anahí Hurtado-Chong, Denise Hess, Vasiliki Kalampoki, Michael Blauth
INTRODUCTION: Treatment of fractures in the elderly population is a clinical challenge due partly to the presence of comorbidities. In a Geriatric Fracture Centre (GFC), patients are co-managed by a geriatrician in an attempt to improve clinical outcomes and reduce morbidity and mortality. Until now the beneficial effect of orthogeriatric co-management has not been definitively proven. The primary objective of this study is to determine the effect of GFC on predefined major adverse events related to a hip fracture compared to usual care centres (UCC)...
July 12, 2017: BMJ Open
https://www.readbyqxmd.com/read/28667646/qpcr-and-hrm-based-diagnosis-of-snps-on-growth-differentiation-factor-9-gdf9-a-gene-associated-with-sheep-ovis-aries-prolificacy
#4
Raquel Anahí Escobar-Chaparro, Gabriel Guillén, Luis Uribe Espejo-Galicia, Víctor Manuel Meza-Villalvazo, Julián Mario Peña-Castro, José Abad-Zavaleta
Prolificacy is a desirable trait for genetic improvement of sheep flocks, since it holds the potential to improve productivity. Animals carrying single-nucleotide polymorphisms (SNPs) in genes associated with this trait can be identified and employed to increase prolificacy in flocks. In this study, we report a diagnostic method based on quantitative PCR and high-resolution melting curves to detect different SNPs in the prolificacy-associated gene growth differentiation factor 9 (GDF9). The diagnostic method was validated using artificial sequences representing known SNPs in GDF9, then applied to a real flock comprising four breeds and admixed animals (n = 306)...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28665011/demographic-model-selection-using-random-forests-and-the-site-frequency-spectrum
#5
Megan L Smith, Megan Ruffley, Anahí Espíndola, David C Tank, Jack Sullivan, Bryan C Carstens
Phylogeographic datasets have grown from tens to thousands of loci in recent years, but extant statistical methods do not take full advantage of these large datasets. For example, Approximate Bayesian Computation (ABC) is a commonly used method for the explicit comparison of alternate demographic histories, but it is limited by the 'curse of dimensionality' and issues related to the simulation and summarization of data when applied to next-generation sequencing (NGS) datasets. We implement here several improvements to overcome these difficulties...
June 30, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28645767/assessing-the-potential-of-rad-sequencing-to-resolve-phylogenetic-relationships-within-species-radiations-the-fly-genus-chiastocheta-diptera-anthomyiidae-as-a-case-study
#6
Tomasz Suchan, Anahí Espíndola, Sereina Rutschmann, Brent C Emerson, Kevin Gori, Christophe Dessimoz, Nils Arrigo, Michał Ronikier, Nadir Alvarez
Determining phylogenetic relationships among recently diverged species has long been a challenge in evolutionary biology. Cytoplasmic DNA markers, which have been widely used, notably in the context of molecular barcoding, have not always proved successful in resolving such phylogenies. However, with the advent of next-generation-sequencing technologies and associated techniques of reduced genome representation, phylogenies of closely related species have been resolved at a much higher detail in the last couple of years...
June 21, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28631376/scale-of-human-mobility-in-the-southern-andes-argentina-and-chile-a-new-framework-based-on-strontium-isotopes
#7
Ramiro Barberena, Víctor A Durán, Paula Novellino, Diego Winocur, Anahí Benítez, Augusto Tessone, María N Quiroga, Erik J Marsh, Alejandra Gasco, Valeria Cortegoso, Gustavo Lucero, Carina Llano, Kelly J Knudson
OBJECTIVES: The goal of this article is to assess the scale of human paleomobility and ecological complementarity between the lowlands and highlands in the southern Andes during the last 2,300 years. By providing isotope results for human bone and teeth samples, we assess a hypothesis of "high residential mobility" suggested on the basis of oxygen isotopes from human remains. METHODS: We develop an isotopic assessment of human mobility in a mountain landscape combining strontium and oxygen isotopes...
June 20, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28603105/antibacterial-activity-of-propolis-extracts-from-the-south-of-portugal
#8
Ana Vanessa Oliveira, Ana Luisa Ferreira, Susana Nunes, Susana Anahi Dandlen, da Graca da Graca Miguel, Maria Leonor Faleiro
To examine the antibacterial activity of diverse extracts of propolis harvested at winter and spring from several locations of Algarve, Portugal, against Gram-negative and Gram-positive bacteria was the main goal of the present work. For such, the antibacterial activity was determined by agar diffusion. The results showed that all tested bacterial strains showed susceptibility to diluted propolis extracts and in a dose-dependent manner. Two propolis samples collected at springtime showed higher antibacterial activity, in comparison with samples harvested at wintertime...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28593502/prognostic-factors-in-surgical-patients-with-chordomas-of-the-cervical-spine-a-study-of-52-cases-from-a-single-institution
#9
Hua Zhou, Liang Jiang, Feng Wei, Alexander Joeris, Anahí Hurtado-Chong, Vasiliki Kalampoki, Elke Rometsch, Miao Yu, Fengliang Wu, Lei Dang, Xiaoguang Liu, Zhongjun Liu
BACKGROUND: The recurrence rate of cervical chordomas is high, and hence it is important to discern the prognostic factors for local relapse and overall survival (OS). METHODS: A retrospective review of 52 patients with cervical chordoma operated in our institution from January 1999 to March 2015 was performed. The association of clinicopathologic characteristics with local relapse-free survival (LRFS) and OS was analyzed. Univariate analysis was performed to determine whether tumor characteristics and types of therapy affected prognosis, and a multivariate Cox proportional hazard model was developed to further investigate local recurrence and mortality...
August 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28583785/late-onset-cogan-s-syndrome-associated-with-large-vessel-vasculitis
#10
Iván Cabezas-Rodríguez, Anahy Brandy-García, Carlos Rodríguez-Balsera, Paloma Rozas-Reyes, Belen Fernández-Llana, Luis Arboleya-Rodríguez
Cogan's syndrome is a rare autoimmune disease that usually affects young Caucasian adults and is classically defined as the combination of nonsyphilitic interstitial keratitis and audiovestibular symptoms resembling Meniere's disease, both of them developed in an interval of less than two years. Nevertheless, cases with atypical ophthalmologic and audiovestibular features, with systemic manifestations or affecting children and older patients have also been reported, expanding the clinical spectrum of Cogan's syndrome...
June 2, 2017: Reumatología Clinica
https://www.readbyqxmd.com/read/28540114/expert-consensus-on-predictive-parameters-for-the-occurrence-of-a-fall-and-a-contralateral-hip-fracture-1-and-3-years-after-hip-fracture-a-delphi-survey
#11
Alexander Joeris, Anahí Hurtado-Chong, Michael Blauth, Joerg Goldhahn, Nicolas S Bodmer, Lucas M Bachmann
BACKGROUND: Early identification of people at risk for a contralateral hip fracture would be desirable to favorably influence patients' prognosis. A recent systematic review failed to depict stringent patterns of risk parameters to be used for decision-making in clinical practice. OBJECTIVE: To perform a consensus study using the Delphi method to reach an expert consensus on predictive parameters for the occurrence of a fall and a contralateral hip fracture 1 and 3 years after hip fracture...
June 2017: Geriatric Orthopaedic Surgery & Rehabilitation
https://www.readbyqxmd.com/read/28524742/piqueria-trinervia-as-a-source-of-metabolites-against-giardia-intestinalis
#12
Yadira Rufino-González, Martha Ponce-Macotela, Manuel Jiménez-Estrada, Candy N Jiménez-Fragoso, Guadalupe Palencia, Gabriel Sansón-Romero, Anahi Anzo-Osorio, Mario N Martínez-Gordillo
CONTEXT: Piqueria trinervia Cav. (Asteraceae) is a plant species with a long history in traditional medicine to cure diarrhoea and other digestive disorders. OBJECTIVE: The study investigates the antigiardial activity of piquerol, trinervinol, red oil and two fractions (F1 and F2) from P. trinervia. MATERIALS AND METHODS: P. trinervia was collected in the Ajusco in Mexico City. Aerial parts were ground and mixed with water to obtain the extract, which was treated with dichloromethane to isolate piquerol and trinervinol (P & T)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28479304/understanding-hematopoietic-stem-cell-development-through-functional-correlation-of-their-proliferative-status-with-the-intra-aortic-cluster-architecture
#13
Antoniana Batsivari, Stanislav Rybtsov, Celine Souilhol, Anahi Binagui-Casas, David Hills, Suling Zhao, Paul Travers, Alexander Medvinsky
During development, hematopoietic stem cells (HSCs) emerge in the aorta-gonad-mesonephros (AGM) region through a process of multi-step maturation and expansion. While proliferation of adult HSCs is implicated in the balance between self-renewal and differentiation, very little is known about the proliferation status of nascent HSCs in the AGM region. Using Fucci reporter mice that enable in vivo visualization of cell-cycle status, we detect increased proliferation during pre-HSC expansion followed by a slowing down of cycling once cells start to acquire a definitive HSC state, similar to fetal liver HSCs...
June 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28473208/-working-conditions-in-outpatient-clinics-adjacent-to-private-pharmacies-in-mexico-city-perspective-of-physicians
#14
Sandra P Díaz-Portillo, Hortensia Reyes-Morales, Silvia Magali Cuadra-Hernández, Álvaro J Idrovo, Gustavo Nigenda, Anahí Dreser
OBJECTIVE: To analyse the working conditions of physicians in outpatient clinics adjacent to pharmacies (CAFs) and their organizational elements from their own perspective. METHODS: We carried out an exploratory qualitative study. Semi-structured interviews were conducted with 32 CAF physicians in Mexico City. A directed content analysis technique was used based on previously built and emerging codes which were related to the experience of the subjects in their work...
May 1, 2017: Gaceta Sanitaria
https://www.readbyqxmd.com/read/28467722/redox-signaling-neuroinflammation-and-neurodegeneration
#15
Gabriela Aguilera, Ana Laura Colín-González, Edgar Rangel-López, Anahí Chavarría, Abel Santamaría
SIGNIFICANCE: Production of pro-inflammatory and anti-inflammatory cytokines is part of the defense system that mostly microglia and macrophages display to induce normal signaling to counteract the deleterious actions of invading pathogens in the brain. Also, redox activity in the central nervous system (CNS) constitutes an integral part of the metabolic processes needed by cells to exert their normal molecular and biochemical functions. Under normal conditions, the formation of reactive oxygen and nitrogen species, and the following oxidative activity encounter a healthy balance with immunological responses to preserve cell functions in the brain...
June 6, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28434066/toxicity-and-genotoxicity-of-three-antimicrobials-commonly-used-in-veterinary-medicine
#16
Anahí Magdaleno, Sofía Carusso, Juan Moretton
The toxicity of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) was tested on two green algal species: the international standard Pseudokirchneriella subcapitata and the native Argentine species Ankistrodesmus fusiformis. All three antibiotics inhibited the algal growth. The most sensitive species was P. subcapitata, for which the EC50 for CTC, OTC and ENF were 1.19 ± 0.53, 0.92 ± 0.30 and 5.18 ± 3.80 mg L(-1), respectively. The EC50 for A. fusiformis, were 3.23 ± 0...
April 22, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28387756/pore-forming-toxin-mediated-ion-dysregulation-leads-to-death-receptor-independent-necroptosis-of-lung-epithelial-cells-during-bacterial-pneumonia
#17
Norberto González-Juarbe, Kelley Margaret Bradley, Anukul Taranath Shenoy, Ryan Paul Gilley, Luis Felipe Reyes, Cecilia Anahí Hinojosa, Marcos Ignacio Restrepo, Peter Herman Dube, Molly Ann Bergman, Carlos Javier Orihuela
We report that pore-forming toxins (PFTs) induce respiratory epithelial cell necroptosis independently of death receptor signaling during bacterial pneumonia. Instead, necroptosis was activated as a result of ion dysregulation arising from membrane permeabilization. PFT-induced necroptosis required RIP1, RIP3 and MLKL, and could be induced in the absence or inhibition of TNFR1, TNFR2 and TLR4 signaling. We detected activated MLKL in the lungs from mice and nonhuman primates experiencing Serratia marcescens and Streptococcus pneumoniae pneumonia, respectively...
May 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28346980/ecological-intensification-to-mitigate-impacts-of-conventional-intensive-land-use-on-pollinators-and-pollination
#18
REVIEW
Anikó Kovács-Hostyánszki, Anahí Espíndola, Adam J Vanbergen, Josef Settele, Claire Kremen, Lynn V Dicks
Worldwide, human appropriation of ecosystems is disrupting plant-pollinator communities and pollination function through habitat conversion and landscape homogenisation. Conversion to agriculture is destroying and degrading semi-natural ecosystems while conventional land-use intensification (e.g. industrial management of large-scale monocultures with high chemical inputs) homogenises landscape structure and quality. Together, these anthropogenic processes reduce the connectivity of populations and erode floral and nesting resources to undermine pollinator abundance and diversity, and ultimately pollination services...
May 2017: Ecology Letters
https://www.readbyqxmd.com/read/28342050/sewage-pollution-genotoxicity-assessment-and-phytoremediation-of-nutrients-excess-with-hydrocotyle-ranunculoides
#19
Gabriel Basílico, Anahí Magdaleno, Marta Paz, Juan Moretton, Ana Faggi, Laura de Cabo
The discharge of sewage effluents into low-order streams has negative effects on water quality. Macrophytes can be efficient in the treatment of this wastewater due to the removal of the main pollutants. The genotoxicity of sewage-polluted water discharging into La Choza stream was evaluated by testing with Allium cepa. Also, a phytoremediation assay with continuous recirculation of the residual water was conducted for 12 days. Three treatments were carried out. One treatment (Hr) was performed with a macrophyte (Hydrocotyle ranunculoides), and two treatments were conducted without macrophytes: with lighting (Ai) and without lighting (Ao)...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28337836/plant-transcription-factors-from-the-homeodomain-leucine-zipper-family-i-role-in-development-and-stress-responses
#20
REVIEW
María Florencia Perotti, Pamela Anahí Ribone, Raquel Lía Chan
In front of stressful conditions plants display adaptation mechanisms leading to changes in their morphology, physiology, development and molecular composition. Transcription factors (TFs) play crucial roles in these complex adaptation processes. This work is focused in the homeodomain-leucine zipper I (HD-Zip I) family of TFs, unique to plants. First discovered in 1991, they were identified and isolated from monocotyledonous and dicotyledonous plants showing high structural similarity and diversified functions...
May 2017: IUBMB Life
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