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https://www.readbyqxmd.com/read/29150726/tspo-imaging-using-the-novel-pet-ligand-18-f-ge-180-quantification-approaches-in-patients-with-multiple-sclerosis
#1
Lena Vomacka, Nathalie Lisa Albert, Simon Lindner, Marcus Unterrainer, Christoph Mahler, Matthias Brendel, Larissa Ermoschkin, Astrid Gosewisch, Anika Brunegraf, Christopher Buckley, Tania Kümpfel, Rainer Rupprecht, Sibylle Ziegler, Martin Kerschensteiner, Peter Bartenstein, Guido Böning
BACKGROUND: PET ligands targeting the translocator protein (TSPO) represent promising tools to visualise neuroinflammation. Here, we analysed parameters obtained in dynamic and static PET images using the novel TSPO ligand [(18)F]GE-180 in patients with relapsing remitting multiple sclerosis (RRMS) and an approach for semi-quantitative assessment of this disease in clinical routine. Seventeen dynamic [(18)F]GE-180 PET scans of RRMS patients were evaluated (90 min). A pseudo-reference region (PRR) was defined after identification of the least disease-affected brain area by voxel-based comparison with six healthy controls (HC) and upon exclusion of voxels suspected of being affected in static 60-90 min p...
October 26, 2017: EJNMMI Research
https://www.readbyqxmd.com/read/29150155/the-association-of-functional-capacity-with-right-atrial-deformation-in-patients-with-pulmonary-arterial-hypertension-a-study-with-two-dimensional-speckle-tracking
#2
Weiwei Liu, Yueheng Wang, Jinling Zhou, Hui Bai, Feng Wang, Ju Wang
BACKGROUND: The purpose of this study was to assess right atrial (RA) myocardial mechanics in pulmonary hypertension (PH) patients using two-dimensional speckle tracking (2D-STE), and define the relationship between RA function and exercise capacity in PH patients. METHODS: Thirty-eight consecutive PH patients were studied and compared with a control group of 25 healthy volunteers. Peak atrial longitudinal strain (PALS), RA strain rate were measured in all subjects...
April 13, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/29149672/the-application-of-bacteriophages-as-novel-indicators-of-viral-pathogens-in-wastewater-treatment-systems
#3
Edgard Dias, James Ebdon, Huw Taylor
Many wastewater treatment technologies have been shown to remove bacterial pathogens more effectively than viral pathogens and, in aquatic environments, levels of traditional faecal indicator bacteria (FIB) do not appear to correlate consistently with levels of human viral pathogens. There is, therefore, a need for novel viral indicators of faecal pollution and surrogates of viral pathogens, especially given the increasing importance of indirect and direct wastewater reuse. Potential candidates include bacteriophages (phages) and the study described here sought to elucidate the relationship between three groups of phages (somatic coliphages (SOMPH), F-RNA coliphages (F-RNAPH) and human-specific phages infecting B...
November 9, 2017: Water Research
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#4
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
November 15, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29147781/a-dft-study-of-a-set-of-natural-dyes-for-organic-electronics
#5
Igo T Lima, Leonardo Sousa, Renato da S Freitas, Luiz Antonio Ribeiro Junior, Rafael Timóteo de Sousa Júnior, Demétrio A da Silva Filho
We systematically investigate, at density functional theory level, the electronic properties of a set of ten carotenoid molecules with different conjugation length. Ground state geometries were fully optimized using both B3LYP and its long-range corrected version, i.e., the CAM-B3LYP functional. The time-dependent DFT approach (TD-DFT) was also performed for the calculation of the excited states of the optimized geometries and the results were compared to the experimental ones, when available. Our findings indicate a dependence of the transition vertical energies, oscillator strengths, and transition dipole moments on the extension of conjugation, as expected...
November 17, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29146327/effect-of-postharvest-spray-of-apple-polyphenols-on-the-quality-of-fresh-cut-red-pitaya-fruit-during-shelf-life
#6
Panhui Fan, Donald J Huber, Zihan Su, Meijiao Hu, Zhaoyin Gao, Min Li, Xuequn Shi, Zhengke Zhang
Fresh-cut (FC) red pitaya fruit were treated with 5ga.i.l(-1) apple polyphenols (APP) and then stored at 20°C for up to 4days to evaluate the effects on attributes. Results showed that FC pitaya fruit with APP treatment showed greater colour retention, delayed softening, reduced loss of soluble solids content, titratable acidity, betacyanin and total phenolics compared with untreated FC fruit. APP treatment also maintained antioxidant activity, as indicated by higher DPPH radical-scavenging activity and reducing power compared with untreated FC pitaya fruit...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29145074/ratiometric-electrochemical-assay-for-sensitive-detecting-microrna-based-on-dual-amplification-mechanism-of-duplex-specific-nuclease-and-hybridization-chain-reaction
#7
Yan-Hong Yuan, Bao-Zhu Chi, Shao-Hua Wen, Ru-Ping Liang, Zhi-Mei Li, Jian-Ding Qiu
We propose a ratiometric electrochemical assay for detecting microRNA (miRNA) on the basis of dual-amplification mechanism by using distinguishable electrochemical signals from thionine (Thi) and ferrocene (Fc). The thiol-modified and ferrocene-labeled hairpin capture probes (CP) are first immobilized on an Au electrode via Au-S reaction. The target miRNA hybridizes with CP and unfolding the hairpin structure of CP to form miRNA-DNA duplexes. Then, kamchatka crab duplex specific nuclease (DSN) specifically cleaves the DNA in miRNA-DNA duplexes, leading to the release of miRNA and another cleaves cycle, meanwhile, numerous Fc leaves away from the electrode surface and leads to the signal-off of Fc...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29144007/analysis-of-surface-roughness-fracture-toughness-and-weibull-characteristics-of-different-framework-veneer-dental-ceramic-assemblies-after-grinding-polishing-and-glazing
#8
Guillermo Pradíes, Laura Godoy-Ruiz, Mutlu Özcan, Isabel Moreno-Hay, Francisco Martínez-Rus
PURPOSE: To compare the surface roughness and biaxial flexural strength of dental ceramics obtained after chairside surface modification by mechanical polishing procedures, versus laboratory reglazing. MATERIALS AND METHODS: Discs (16 × 1.5 ± 1.6 mm) (N = 90) of various framework-veneering combinations were fabricated: D/FC: lithium disilicate/feldspathic ceramic; Z/AL: zirconium dioxide/aluminous ceramic; N/FC: noble alloy/feldspathic ceramic; N/FF: noble alloy feldspathic with fluorapatite; B/FC: base alloy/feldspathic ceramic; B/FF: base alloy/feldspathic ceramic with fluorapatite...
November 16, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/29143175/vectors-for-expression-of-signal-peptide-dependent-proteins-in-baculovirus-insect-cell-systems-and-their-application-to-expression-and-purification-of-the-high-affinity-immunoglobulin-gamma-fc-receptor-i-in-complex-with-its-gamma-chain
#9
Le T M Le, Jens R Nyengaard, Monika M Golas, Bjoern Sander
Integral membrane proteins play a central role in various cellular functions and are important therapeutic targets. However, technical challenges in the overexpression and purification of membrane proteins often represent a limiting factor for biochemical and structural studies. Here, we constructed a set of vectors, derivatives of MultiBac vectors that can be used to express proteins with a cleavable N-terminal signal peptide in insect cells. We propose these vectors for expression of type I membrane proteins and other secretory pathway proteins that require the signal recognition particle for translocation to the endoplasmic reticulum (ER)...
November 15, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29140180/the-role-of-lumbopelvic-hip-complex-stability-in-softball-throwing-mechanics
#10
Gabrielle G Gilmer, Jessica K Washington, Jeffrey Dugas, James Andrews, Gretchen D Oliver
CONTEXT: Studies have found that a 20% reduction in energy generation from the lumbopelvic-hip complex (LPHC) during overhead throws leads to a 34% increase in load on the shoulder. OBJECTIVE: The purpose of this study was to assess the effects of LPHC stability, via the single leg squat (SLS) assessment, on throwing mechanics of softball athletes. DESIGN: Prospective cohort study. SETTING: Laboratory setting. PARTICIPANTS: Fifty softball athletes (164...
November 15, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/29140158/endosomal-trafficking-regulates-receptor-mediated-transcytosis-of-antibodies-across-the-blood-brain-barrier
#11
Arsalan S Haqqani, Christie E Delaney, Eric Brunette, Ewa Baumann, Graham K Farrington, William Sisk, John Eldredge, Wen Ding, Tammy-Lynn Tremblay, Danica B Stanimirovic
Current methods for examining antibody trafficking are either non-quantitative such as immunocytochemistry or require antibody labeling with tracers. We have developed a multiplexed quantitative method for antibody 'tracking' in endosomal compartments of brain endothelial cells. Rat brain endothelial cells were co-incubated with blood-brain barrier (BBB)-crossing FC5, monovalent FC5Fc or bivalent FC5Fc fusion antibodies and control antibodies. Endosomes were separated using sucrose-density gradient ultracentrifugation and analyzed using multiplexed mass spectrometry to simultaneously quantify endosomal markers, receptor-mediated transcytosis (RMT) receptors and the co-incubated antibodies in each fraction...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29139548/prospects-from-systems-serology-research
#12
REVIEW
Kelly B Arnold, Amy W Chung
Antibodies are highly functional glycoproteins capable of providing immune protection through multiple mechanisms, including direct pathogen neutralization and the engagement of their Fc-portions with surrounding effector immune cells and immune components that induce anti-pathogenic responses. Small modifications to multiple antibody biophysical features induced by vaccines and other therapeutic regimens can significantly alter functional immune outcomes, though it is difficult to predict which combinations confer protective immunity...
November 15, 2017: Immunology
https://www.readbyqxmd.com/read/29139314/cost-utility-analysis-of-life-long-prophylaxis-with-recombinant-factor-viii-fc-vs-recombinant-factor-viii-for-the-management-of-severe-haemophilia-a-in-sweden
#13
Nathaniel Henry, Jelena Jovanović, Max Schlueter, Persefoni Kritikou, Koo Wilson, Karl-Johan Myrén
AIMS: Prophylaxis with recombinant factor VIII (rFVIII) is the standard of care for severe haemophilia A in Sweden. The need for frequent injections with existing rFVIII products may, however, result in poor adherence to prophylaxis leading to increased bleeding and long-term joint damage. Recombinant FVIIIFc (rFVIIIFc) is an extended half-life fusion protein which can offer prolonged protection and reduced dosing frequency. The objective of this study was to evaluate the cost-utility of prophylaxis with rFVIIIFc in severe haemophilia A from the perspective of the Swedish health system...
November 15, 2017: Journal of Medical Economics
https://www.readbyqxmd.com/read/29138497/rapid-purification-of-human-bispecific-antibodies-via-selective-modulation-of-protein-a-binding
#14
Adam Zwolak, Catherine N Leettola, Susan H Tam, Dennis R Goulet, Mehabaw G Derebe, Jose R Pardinas, Songmao Zheng, Rose Decker, Eva Emmell, Mark L Chiu
Methods to rapidly generate high quality bispecific antibodies (BsAb) having normal half-lives are critical for therapeutic programs. Here, we identify 3 mutations (T307P, L309Q, and Q311R or "TLQ") in the Fc region of human IgG1 which disrupt interaction with protein A while enhancing interaction with FcRn. The mutations are shown to incrementally alter the pH at which a mAb elutes from protein A affinity resin. A BsAb comprised of a TLQ mutant and a wild-type IgG1 can be efficiently separated from contaminating parental mAbs by differential protein A elution starting from either a) purified parental mAbs, b) in-supernatant crossed parental mAbs, or c) co-transfected mAbs...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137283/c1q-tumor-necrosis-factor-related-protein-3-protects-macrophages-against-lps-induced-lipid-accumulation-inflammation-and-phenotype-transition-via-ppar%C3%AE-and-tlr4-mediated-pathways
#15
Jiale Lin, Qi Liu, Hui Zhang, Xingtao Huang, Ruoxi Zhang, Shuyuan Chen, Xuedong Wang, Bo Yu, Jingbo Hou
Macrophage inflammation and foam cell formation are critical events during the initiation and development of atherosclerosis (AS). C1q/tumor necrosis factor-related protein-3 (CTRP3) is a novel adipokine with anti-inflammatory and cardioprotection properties; however, little is known regarding the influence of CTRP3 on AS. As macrophages play a key role in AS, this study investigated the effects of CTRP3 on macrophage lipid metabolism, inflammatory reactions, and phenotype transition, as well as underlying mechanisms, to reveal the relationship between CTRP3 and AS...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137228/a-native-like-bispecific-antibody-suppresses-the-inflammatory-cytokine-response-by-simultaneously-neutralizing-tumor-necrosis-factor-alpha-and-interleukin-17a
#16
Tianshu Xu, Tianlei Ying, Lili Wang, Xiaohua Douglas Zhang, Ying Wang, Lishan Kang, Tao Huang, Liang Cheng, Liping Wang, Qi Zhao
Anti-tumor necrosis factor (TNF) therapies are successful in the treatment of inflammatory disorders. However, some patients with rheumatoid arthritis (RA) fail to response anti-TNF drugs due to the compensation of other inflammatory signals. In this study, to reduce compensatory responses of interleukin-17A (IL-17A) during TNF-α inhibition, we generated an IgG-like bispecific antibodiy (bsAb) against TNF-α and IL-17A through a combination method of electrostatic Fc pairing and light chain crossover. This bsAb exhibited relatively high stability comparable to natural IgG antibodies, and retained the unaltered affinities to both of two targets...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136712/-long-term-outcome-and-prognostic-factors-in-pregnant-women-with-pulmonary-arterial-hypertension-associated-with-congenital-heart-disease
#17
Q T Ou, J K Lu, J Zhang, Y Chen, Q Li, J L Zhang
Objective: To investigate the perinatal outcome, risk factors and long-term outcome of pregnancy complicated with pulmonary arterial hypertension(PAH) and congenital heart diseases (CHD). Methods: Clinical data of 110 pregnant women who were diagnosed as PAH-CHD were retrospectively analyzed in the Department of Obstetrics and Gynecology and Surgical Intensive Care Unit at Beijing Anzhen Hospital from 2004 to 2013. The survival and treatment status were followed up. Results: 110 subjects consisted of 11 mild PAH, 33 moderate and 66 severe ones...
November 1, 2017: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/29136384/field-effect-modulation-of-heterogeneous-charge-transfer-kinetics-at-back-gated-2d-mos2-electrodes
#18
Yan Wang, Chang-Hyun Kim, Youngdong Yoo, James E Johns, C Daniel Frisbie
The ability to improve and to modulate the heterogeneous charge transfer kinetics of two dimensional (2D) semiconductors, such as MoS2, is a major challenge for electrochemical and photoelectrochemical applications of these materials. Here we report a continuous and reversible physical method for modulating the heterogeneous charge transfer kinetics at a monolayer MoS2 working electrode supported on a SiO2/p-Si substrate. The heavily doped p-Si substrate serves as a back gate electrode; application of a gate voltage (VBG) to p-Si tunes the electron occupation in the MoS2 conduction band and shifts the conduction band edge position relative to redox species dissolved in electrolyte in contact with the front side of the MoS2...
November 14, 2017: Nano Letters
https://www.readbyqxmd.com/read/29136125/effective-connectivity-measured-using-optogenetically-evoked-hemodynamic-signals-exhibits-topography-distinct-from-resting-state-functional-connectivity-in-the-mouse
#19
Adam Q Bauer, Andrew W Kraft, Grant A Baxter, Patrick W Wright, Matthew D Reisman, Annie R Bice, Jasmine J Park, Michael R Bruchas, Abraham Z Snyder, Jin-Moo Lee, Joseph P Culver
Brain connectomics has expanded from histological assessment of axonal projection connectivity (APC) to encompass resting state functional connectivity (RS-FC). RS-FC analyses are efficient for whole-brain mapping, but attempts to explain aspects of RS-FC (e.g., interhemispheric RS-FC) based on APC have been only partially successful. Neuroimaging with hemoglobin alone lacks specificity for determining how activity in a population of cells contributes to RS-FC. Wide-field mapping of optogenetically defined connectivity could provide insights into the brain's structure-function relationship...
November 9, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/29135944/design-synthesis-and-use-of-peptides-derived-from-human-papillomavirus-l1-protein-for-the-modification-of-gold-electrode-surfaces-by-self-assembled-monolayers
#20
John Alejandro Lara Carrillo, Ricardo Fierro Medina, Juan Manríquez Rocha, Erika Bustos Bustos, Diego Sebastián Insuasty Cepeda, Javier Eduardo García Castañeda, Zuly Jenny Rivera Monroy
In order to obtain gold electrode surfaces modified with Human Papillomavirus L1 protein (HPV L1)-derived peptides, two sequences, SPINNTKPHEAR and YIK, were chosen. Both have been recognized by means of sera from patients infected with HPV. The molecules, Fc-Ahx-SPINNTKPHEAR, Ac-C-Ahx-(Fc)KSPINNTKPHEAR, Ac-C-Ahx-SPINNTKPHEAR(Fc)K, C-Ahx-SPINNTKPHEAR, and (YIK)₂-Ahx-C, were designed, synthesized, and characterized. Our results suggest that peptides derived from the SPINNTKPHEAR sequence, containing ferrocene and cysteine residues, are not stable and not adequate for electrode surface modification...
November 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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