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https://www.readbyqxmd.com/read/28929761/the-reaction-of-methyl-fluoroformyl-peroxycarbonate-fc-o-ooc-o-och3-with-cl-atoms-formation-of-hydro-chlorofluoro-peroxides
#1
Matias Berasategui, Gustavo Alejandro Argüello, Maxi Alberto Burgos Paci
The products following Cl atom initiated reactions of FC(O)OOC(O)OCH3 in 50-760 Torr of N2 at 296 K were investigated using FTIR. Reaction of Cl atoms with methyl fluoroformyl peroxycarbonate proceeds mainly via attack at the methyl group, forming FC(O)OOC(O)OCH2˙ radicals. Further reaction of this kind of radicals with Cl2 forms three new compounds: FC(O)OOC(O)OCH2Cl; FC(O)OOC(O)OCHCl2 and FC(O)OOC(O)OCCl3, whose existence was characterized experimentally by FTIR spectroscopy assisted with ab-initio calculations at the B3LYP/6-31++G(d,p) level...
September 20, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28929587/antibody-based-strategies-for-the-detection-of-luspatercept-ace-536-in-human-serum
#2
Christian Reichel, G Gmeiner, Mario Thevis
Luspatercept (ACE-536, ACVR2B-Fc), a fusion protein consisting of the extracellular domain of ActRIIB receptor and the Fc-part of human immunoglobulin G1 (IgG1), is currently under clinical development (Phase III). It stimulates the formation of red blood cells and hence may be misused by athletes for doping purposes in the future. Several antibody-based strategies for the detection of Luspatercept and other ACVR2B-Fc fusion proteins in human serum were evaluated (ELISA; IEF-, SDS-, and SAR-PAGE followed by Western blotting; immunoprecipitation)...
September 19, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28928828/screening-of-candidate-key-genes-associated-with-human-osteosarcoma-using-bioinformatics-analysis
#3
Kefeng Zhang, Jianwen Gao, Yong Ni
The aim of the present study was to identify the key genes associated with osteosarcoma (OS) using a bioinformatics approach. Microarray data (GSE36004) was downloaded from the Gene Expression Omnibus database, including 19 OS cell lines and 6 normal controls. Differentially expressed genes (DEGs) in the OS cell lines were identified using the Limma package, and differentially methylated regions were screened with methyAnalysis in R. Copy number analysis was performed and genes with copy number gains/losses were further screened using DNAcopy and cghMCR packages...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28928748/a-peptide-fc-opsonin-with-pan-amyloid-reactivity
#4
James S Foster, Angela D Williams, Sallie Macy, Tina Richey, Alan Stuckey, Daniel Craig Wooliver, Richa Koul-Tiwari, Emily B Martin, Stephen J Kennel, Jonathan S Wall
There is a continuing need for therapeutic interventions for patients with the protein misfolding disorders that result in systemic amyloidosis. Recently, specific antibodies have been employed to treat AL amyloidosis by opsonizing tissue amyloid deposits thereby inducing cell-mediated dissolution and organ improvement. To develop a pan-amyloid therapeutic agent, we have produced an Fc-fusion product incorporating a peptide, p5, which binds many if not all forms of amyloid. This protein, designated Fcp5, expressed in mammalian cells, forms the desired bivalent dimer structure and retains pan-amyloid reactivity similar to the p5 peptide as measured by immunosorbent assays, immunohistochemistry, surface plasmon resonance, and pulldown assays using radioiodinated Fcp5...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28928215/influenza-virus-hemagglutinin-stalk-specific-antibodies-in-human-serum-are-a-surrogate-marker-for-in-vivo-protection-in-a-serum-transfer-mouse-challenge-model
#5
Henning Jacobsen, Madhusudan Rajendran, Angela Choi, Haakon Sjursen, Karl A Brokstad, Rebecca J Cox, Peter Palese, Florian Krammer, Raffael Nachbagauer
The immunogenicity of current influenza virus vaccines is assessed by measuring an increase of influenza virus-specific antibodies in a hemagglutination inhibition assay. This method exclusively measures antibodies against the hemagglutinin head domain. While this domain is immunodominant, it has been shown that hemagglutination inhibition titers do not always accurately predict protection from disease. In addition, several novel influenza virus vaccines that are currently under development do not target the hemagglutinin head domain, but rather more conserved sites, including the hemagglutinin stalk...
September 19, 2017: MBio
https://www.readbyqxmd.com/read/28927728/cryopreservation-of-human-whole-blood-allows-immunophenotyping-by-flow-cytometry-up-to-30days-after-cell-isolation
#6
R Madelaine Paredes, Douglas K Tadaki, Amanda Sooter, Fabia Gamboni, C D R Forest Sheppard
Immunophenotyping of whole blood (WB) by flow cytometry (FC) is used clinically to assess a patient's immune status and also in biomedical research. Current protocols recommend storage of immunolabeled samples at 4°C with FC analysis to be completed within seven days. This data acquisition window can be extended to up to one year post-labeling, but this requires cryopreservation of the samples at ultra-low temperatures (≤-80°C or in liquid nitrogen). In this study we optimized a standardized cryopreservation protocol to enable preservation of immunolabeled, human WB samples at -20°C for FC and tested its effectiveness after 0, 5, 15 or 30days...
September 16, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28927270/proteomic-analysis-of-peripheral-blood-mononuclear-cells-pbmc-after-a-high-fat-high-carbohydrate-meal-with-orange-juice
#7
Daniela F Seixas Chaves, Paulo C Carvalho, Elisa Brasili, Marcelo Macedo Rogero, Neuza Mariko Aymoto Hassimotto, Jolene K Diedrich, James J Moresco, John R Yates, Franco Maria Lajolo
Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown that orange juice is able to prevent this response by inhibiting toll like receptors (TLR) expression and endotoxemia. Our goal was to study the proteome response in PBMC after the consumption of a HFHC meal consumed with water, orange juice or an isocaloric beverage (water with glucose)...
September 19, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28926673/combinatorial-genome-and-protein-engineering-yields-monoclonal-antibodies-with-hypergalactosylation-from-cho-cells
#8
Cheng-Yu Chung, Qiong Wang, Shuang Yang, Sean A Ponce, Brian J Kirsch, Hui Zhang, Michael J Betenbaugh
One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution. Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies. However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities. In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms...
July 7, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28926542/corrigendum-igg-fc-domains-that-bind-c1q-but-not-effector-fc%C3%AE-receptors-delineate-the-importance-of-complement-mediated-effector-functions
#9
Chang-Han Lee, Gabrielle Romain, Wupeng Yan, Makiko Watanabe, Wissam Charab, Biliana Todorova, Jiwon Lee, Kendra Triplett, Moses Donkor, Oana I Lungu, Anja Lux, Nicholas Marshall, Margaret A Lindorfer, Odile Richard-Le Goff, Bianca Balbino, Tae Hyun Kang, Hidetaka Tanno, George Delidakis, Corrine Alford, Ronald P Taylor, Falk Nimmerjahn, Navin Varadarajan, Pierre Bruhns, Yan Jessie Zhang, George Georgiou
No abstract text is available yet for this article.
September 19, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28926475/altered-resting-state-functional-connectivity-of-default-mode-network-and-sensorimotor-network-in-heavy-metal-music-lovers
#10
Yan Sun, Congcong Zhang, Shuxia Duan, Xiaoxia Du, Vince D Calhoun
The aim of this study was to investigate the spontaneous neural activity and functional connectivity (FC) in heavy metal music lovers (HMML) compared with classical music lovers (CML) during resting state. Forty HMML and 31 CML underwent resting-state functional MRI scans. Fractional amplitude of low-frequency fluctuations (fALFF) and seed-based resting-state FC were computed to explore regional activity and functional integration. A voxel-based two-sample t-test was used to test the differences between the two groups...
September 18, 2017: Neuroreport
https://www.readbyqxmd.com/read/28925591/melinjo-gnetum-gnemon-seed-extract-consumption-during-lactation-improved-vasodilation-and-attenuated-the-development-of-hypertension-in-female-offspring-of-fructose-fed-pregnant-rats
#11
Rachael A Uson-Lopez, Saori Kataoka, Yuuka Mukai, Shin Sato, Masaaki Kurasaki
BACKGROUND: Fructose intake has been correlated with increased prevalence of metabolic disorders including hypertension. In pregnant rats, fructose intake has been reported to have adverse effects on the health of its offspring. This study investigated the effects of gestational maternal fructose consumption and if supplementation with melinjo seed extracts to the maternal diet during lactation could benefit the offspring in later life. METHODS: Pregnant rats were randomly divided into three groups: untreated (CC), fructose-treated (FC), and fructose and melinjo-treated (FM)...
September 19, 2017: Birth defects research
https://www.readbyqxmd.com/read/28924691/pharmacokinetic-considerations-for-antibody-drug-conjugates-against-cancer
#12
REVIEW
Paul Malik, Colin Phipps, Andrea Edginton, Jonathan Blay
Antibody-drug conjugates (ADCs) are ushering in the next era of targeted therapy against cancer. An ADC for cancer therapy consists of a potent cytotoxic payload that is attached to a tumour-targeted antibody by a chemical linker, usually with an average drug-to-antibody ratio (DAR) of 3.5-4. The theory is to deliver potent cytotoxic payloads directly to tumour cells while sparing healthy cells. However, practical application has proven to be more difficult. At present there are only two ADCs approved for clinical use...
September 18, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28923156/functional-constructivism-in-search-of-formal-descriptors
#13
Irina Trofimova
The Functional Constructivism (FC) paradigm is an alternative to behaviorism and considers behavior as being generated every time anew, based on an individual's capacities, environmental resources and demands. Walter Freeman's work provided us with evidence supporting the FC principles. In this paper we make parallels between gradual construction processes leading to the formation of individual behavior and habits, and evolutionary processes leading to the establishment of biological systems. Referencing evolutionary theory, several formal descriptors of such processes are proposed...
October 2017: Nonlinear Dynamics, Psychology, and Life Sciences
https://www.readbyqxmd.com/read/28921546/neuroblastoma-cells-undergo-transcriptomic-alterations-upon-dissemination-into-the-bone-marrow-and-subsequent-tumor-progression
#14
Fikret Rifatbegovic, Christian Frech, M Reza Abbasi, Sabine Taschner-Mandl, Tamara Weiss, Wolfgang M Schmidt, Iris Schmidt, Ruth Ladenstein, Inge M Ambros, Peter F Ambros
Neuroblastoma is the most common extracranial solid tumor in childhood. The vast majority of metastatic (M) stage patients present with disseminated tumor cells (DTCs) in the bone marrow (BM) at diagnosis and relapse. Although these cells represent a major obstacle in the treatment of neuroblastoma patients, insights into their expression profile remained elusive. The present RNA-Seq study of stage 4/M primary tumors, enriched BM-derived diagnostic and relapse DTCs, as well as the corresponding BM-derived mononuclear cells (MNCs) from 53 patients revealed 322 differentially expressed genes in DTCs as compared to the tumors (q < 0...
September 16, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28921534/exploring-cellular-behaviour-under-transient-gene-expression-and-its-impact-on-mab-productivity-and-fc-glycosylation
#15
Si Nga Sou, Ken Lee, Kalpana Nayyar, Karen M Polizzi, Christopher Sellick, Cleo Kontoravdi
Transient gene expression (TGE) is a methodology employed in bioprocessing for the fast provision of recombinant protein material. Mild hypothermia is often introduced to overcome the low yield typically achieved with TGE and improve specific protein productivity. It is therefore of interest to examine the impact of mild hypothermic temperatures on both the yield and quality of transiently-expressed proteins and the relationship to changes in cellular processes and metabolism. In this study, we focus on the ability of a Chinese hamster ovary cell line to galactosylate a recombinant monoclonal antibody (mAb) product...
September 16, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28921389/altered-structure-and-intrinsic-functional-connectivity-in-post-stroke-aphasia
#16
Mi Yang, Pu Yang, Yun-Shuang Fan, Jiao Li, Dezhong Yao, Wei Liao, Huafu Chen
Previous studies have demonstrated that alterations of gray matter exist in post-stroke aphasia (PSA) patients. However, so far, few studies combined structural alterations of gray matter volume (GMV) and intrinsic functional connectivity (iFC) imbalances of resting-state functional MRI to investigate the mechanism underlying PSA. The present study investigated specific regions with GMV abnormality in patients with PSA (n = 17) and age- and sex- matched healthy controls (HCs, n = 20) using voxel-based morphometry...
September 18, 2017: Brain Topography
https://www.readbyqxmd.com/read/28920405/effects-of-processing-particle-size-and-moisturizing-of-sorghum-based-feeds-on-pellet-quality-and-broiler-production
#17
Patrícia Garcia Silva, Fabiana Ramos Santos, Luana Martins Schaly Oliveira, Nayanne Rodrigues Oliveira, Fábio Ataides Moura Júnior, Maura Regina Sousa Silva, Deibity Alves Cordeiro, Cibele Silva Minafra
Objective: This study aimed to assess the effect of pelleted and expanded sorghum-based feeds prepared with different moisture levels and particle size of ingredients on metabolizable energy, ileal digestibility of amino acids and broiler performance. Methods: The experiment was performed with 720 male broiler chicks of the Cobb strain, with treatments of six replications, with 15 birds each; they were arranged in a completely randomized design and 2 × 2 × 2 factorial scheme (pelleted or expanded feed processing, 0...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28919849/interhemispheric-resting-state-functional-connectivity-predicts-severity-of-idiopathic-normal-pressure-hydrocephalus
#18
Yousuke Ogata, Akihiko Ozaki, Miho Ota, Yurie Oka, Namiko Nishida, Hayato Tabu, Noriko Sato, Takashi Hanakawa
Idiopathic normal pressure hydrocephalus (iNPH) is characterized by a clinical triad (gait disturbance, dementia, and urinary incontinence), and by radiological findings of enlarged ventricles reflecting disturbance of central spinal fluid circulation. A diagnosis of iNPH is sometimes challenging, and the pathophysiological mechanisms underlying the clinical symptoms of iNPH remain largely unknown. Here, we used an emerging MRI technique, resting-state functional connectivity MRI (rsfcMRI), to develop a subsidiary diagnostic technique and to explore the underlying pathophysiological mechanisms of iNPH...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28919388/altered-brain-functional-connectivity-induced-by-physical-exercise-may-improve-neuropsychological-functions-in-patients-with-benign-epilepsy
#19
Gyan Raj Koirala, Dongpyo Lee, Soyong Eom, Nam-Young Kim, Heung Dong Kim
OBJECTIVE: The objective of this study was to elucidate alteration in functional connectivity (FC) in patients with benign epilepsy with centrotemporal spikes (BECTS) as induced by physical exercise therapy and their correlation to the neuropsychological (NP) functions. METHODS: We analyzed 115 artifact- and spike-free 2-second epochs extracted from resting state EEG recordings before and after 5weeks of physical exercise in eight patients with BECTS. The exact Low Resolution Electromagnetic Tomography (eLORETA) was used for source reconstruction...
September 15, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28918477/in-vitro-inhibition-of-hiv-1-replication-in-autologous-cd4-t-cells-indicates-viral-containment-by-multifactorial-mechanisms
#20
Ting Tu, Jianbo Zhan, Danlei Mou, Wei Li, Bin Su, Tong Zhang, Tao Li, Ning Li, Hao Wu, Cong Jin, Huabiao Chen
HIV-1-specific cytotoxic T lymphocytes (CTLs) and neutralizing antibodies (NAbs) are present during chronic infection, but the relative contributions of these effector mechanisms to viral containment remain unclear. Here, using an in vitro model involving autologous CD4(+) T cells, primary HIV-1 isolates, HIV-1-specific CTLs, and neutralizing monoclonal antibodies, we show that b12, a potent and broadly neutralizing monoclonal antibody to HIV-1, was able to block viral infection when preincubated with virus prior to infection, but was much less effective than CTLs at limiting virus replication when added to infected cell cultures...
September 15, 2017: Virologica Sinica
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