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intradiscal stem cell

Hemant Kumar, Doo-Hoe Ha, Eun-Jong Lee, Jun Hee Park, Jeong Hyun Shim, Tae-Keun Ahn, Kyoung-Tae Kim, Alexander E Ropper, Seil Sohn, Chung-Hun Kim, Devang Kashyap Thakor, Soo-Hong Lee, In-Bo Han
BACKGROUND: Adipose tissue-derived mesenchymal stem cells (AT-MSCs) offer potential as a therapeutic option for chronic discogenic low back pain (LBP) because of their immunomodulatory functions and capacity for cartilage differentiation. The goal of this study was to assess the safety and tolerability of a single intradiscal implantation of combined AT-MSCs and hyaluronic acid (HA) derivative in patients with chronic discogenic LBP. METHODS: We performed a single-arm phase I clinical trial with a 12-month follow-up and enrolled 10 eligible chronic LBP patients...
November 15, 2017: Stem Cell Research & Therapy
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
Christopher Centeno, Jason Markle, Ehren Dodson, Ian Stemper, Christopher J Williams, Matthew Hyzy, Thomas Ichim, Michael Freeman
BACKGROUND: Degenerative disc disease (DDD) is a common cause of lower back pain with radicular symptoms and has a significant socioeconomic impact given the associated disability. Limited effective conservative therapeutic options result in many turning to surgical alternatives for management, which vary in the rate of success and also carry an increased risk of morbidity and mortality associated with the procedures. Several animal based studies and a few human pilot studies have demonstrated safety and suggest efficacy in the treatment of DDD with mesenchymal stem cells (MSCs)...
September 22, 2017: Journal of Translational Medicine
Xiaofei Cheng, Guoying Zhang, Liang Zhang, Ying Hu, Kai Zhang, Xiaojiang Sun, Changqing Zhao, Hua Li, Yan Michael Li, Jie Zhao
Although mesenchymal stem cells (MSCs) transplantation into the IVD (intervertebral disc) may be beneficial in inhibiting apoptosis of nucleus pulposus cells (NPCs) and alleviating IVD degeneration, the underlying mechanism of this therapeutic process has not been fully explained. The purpose of this study was to explore the protective effect of MSC-derived exosomes (MSC-exosomes) on NPC apoptosis and IVD degeneration and investigate the regulatory effect of miRNAs in MSC-exosomes and associated mechanisms for NPC apoptosis...
August 14, 2017: Journal of Cellular and Molecular Medicine
Sarah E Gullbrand, Thomas P Schaer, Prateek Agarwal, Justin R Bendigo, George R Dodge, Weiliam Chen, Dawn M Elliott, Robert L Mauck, Neil R Malhotra, Lachlan J Smith
Degeneration of the intervertebral discs is a progressive cascade of cellular, compositional and structural changes that is frequently associated with low back pain. As the first signs of disc degeneration typically arise in the disc's central nucleus pulposus (NP), augmentation of the NP via hydrogel injection represents a promising strategy to treat early to mid-stage degeneration. The purpose of this study was to establish the translational feasibility of a triple interpenetrating network hydrogel composed of dextran, chitosan, and teleostean (DCT) for augmentation of the degenerative NP in a preclinical goat model...
September 15, 2017: Acta Biomaterialia
Nebojsa Nick Knezevic, Shane Mandalia, Jennifer Raasch, Ivana Knezevic, Kenneth D Candido
Back pain is the second leading cause of disability among American adults and is currently treated either with conservative therapy or interventional pain procedures. However, the question that remains is whether we, as physicians, have adequate therapeutic options to offer to the patients who suffer from chronic low back pain but fail both conservative therapy and interventional pain procedures before they consider surgical options such as discectomy, disc arthroplasty, or spinal fusion. The purpose of this article is to review the potential novel therapies that are on the horizon for the treatment of chronic low back pain...
2017: Journal of Pain Research
Frank Steffen, Lucas Adam Smolders, Anne Marie Roentgen, Alessandro Bertolo, Jivko Stoyanov
The intradiscal application of mesenchymal stem cells (MSCs) is considered a promising strategy for intervertebral disc (IVD) regeneration. Although many studies have been published, the feasibility and regenerative effects of intradiscal MSC application have not been evaluated in an animal model suffering from naturally occurring degenerative disc disease. Six German Shepherd Dogs suffering from naturally occurring degenerative IVD disease were included. Autologous MSCs were isolated from bone marrow (iliac crest) and cultured for 3 weeks...
November 2017: Tissue Engineering. Part C, Methods
M Basso, L Cavagnaro, A Zanirato, S Divano, C Formica, M Formica, L Felli
PURPOSE: This review aims to explore and summarize the current clinical evidence about the use of regenerative medicine such as mesenchymal stem cells or platelet-rich plasma in intervertebral disc regeneration, in order to clarify the state of art of these novel approaches. MATERIALS AND METHODS: We performed a research of the available literature about regenerative medicine strategies aiming to prevent intervertebral disc degeneration. All preclinical trials and in vitro studies were excluded...
August 2017: Musculoskeletal Surgery
Rodrigo Caldonazzo Fávaro, André de Oliveira Arruda, Luiz Roberto Gomes Vialle, Emiliano Neves Vialle
OBJECTIVE: The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury. METHODS: 32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest versus saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP)...
November 2016: Revista Brasileira de Ortopedia
Kenneth Pettine, Richard Suzuki, Theodore Sand, Matthew Murphy
PURPOSE: The purpose of this study is to assess safety and feasibility of intradiscal bone marrow concentrate (BMC) injections to treat discogenic pain as an alternative to surgery. METHODS: A total of 26 patients (11 male, 15 female, aged 18-61 years, 13 single level, 13 two level) that met inclusion criteria of chronic (> 6 months) discogenic low back pain, degenerative disc pathology assessed by magnetic resonance imaging (MRI) with modified Pfirrmann grade of IV-VII at one or two levels, candidate for surgical intervention (failed conservative treatment and radiologic findings) and a visual analogue scale (VAS) pain score of 40 mm or more at initial visit...
January 2016: International Orthopaedics
Giovanni Marfia, Rolando Campanella, Stefania Elena Navone, Ileana Zucca, Alessandro Scotti, Matteo Figini, Clara Di Vito, Giulio Alessandri, Laura Riboni, Eugenio Parati
INTRODUCTION: Biglycan is an important proteoglycan of the extracellular matrix of intervertebral disc (IVD), and its decrease with aging has been correlated with IVD degeneration. Biglycan deficient (Bgn-/0) mice lack this protein and undergo spontaneous IVD degeneration with aging, thus representing a valuable in vivo model for preliminary studies on therapies for human progressive IVD degeneration. The purpose of the present study was to assess the possible beneficial effects of adipose-derived stromal cells (ADSCs) implants in the Bgn-/0 mouse model...
October 8, 2014: Arthritis Research & Therapy
Gianluca Fontana, Akshay Srivastava, Dilip Thomas, Pierce Lalor, Peter Dockery, Abhay Pandit
Intradiscal injection of growth factors or cells has been shown to attenuate symptoms of intervertebral disc degeneration. However, different approaches are needed to overcome limitations such as short-term efficacy and leakage of the injected solutions. The current study aims at creating a platform for the realization of functional cell factories by using in parallel cell delivery and gene therapy approaches. Superfect, a transfecting agent, was used as nonviral gene vector because of its ability to form complexes with plasmid DNA (polyplexes)...
July 15, 2015: Bioconjugate Chemistry
G W Omlor, J Fischer, K Kleinschmitt, K Benz, J Holschbach, K Brohm, M Anton, T Guehring, W Richter
PURPOSE: Cell therapy would be favorably performed immediately after nucleotomy, to restore intervertebral disc functionality and to slow down disc degeneration. Promising results were reported from small animal models but remaining problems, especially in larger animals, include loss of vital cells due to annular damage at the injection site and detrimental intradiscal conditions. The aim of the present study was to optimize cell-based disc therapy using a new albumin-hyaluronan hydrogel together with bone marrow-derived mesenchymal stem cells in a large porcine disc model...
September 2014: European Spine Journal
Vivian Tam, Ian Rogers, Danny Chan, Victor Y L Leung, Kenneth M C Cheung
A major hurdle of cellular therapy for biological treatment of intervertebral disk (IVD) degeneration is the delivery method where current delivery methods are limited to intradiscal injection which can potentially cause further degeneration. Recent studies indicated that multipotential stem cells (MPSCs) from human umbilical cord blood home to injured sites and induce local therapeutic changes, thereby potentially addressing the drawbacks of direct delivery. We tested the effects of these cells on injured IVD using a mouse model of puncture-induced degeneration via two delivery methods...
June 2014: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Yuk Yin Li, Hua Jia Diao, Tze Kit Chik, Cin Ting Chow, Xiao Meng An, Victor Leung, Kenneth Man Chi Cheung, Barbara Pui Chan
Mesenchymal stem cells (MSCs) have the potential to treat early intervertebral disc (IVD) degeneration. However, during intradiscal injection, the vast majority of cells leaked out even in the presence of hydrogel carrier. Recent evidence suggests that annulus puncture is associated with cell leakage and contributes to osteophyte formation, an undesirable side effect. This suggests the significance of developing appropriate carriers for intradiscal delivery of MSCs. We previously developed a collagen microencapsulation platform, which entraps MSCs in a solid microsphere consisting of collagen nanofiber meshwork...
May 2014: Tissue Engineering. Part A
Brian R Subach, Anne G Copay, Marcus M Martin, Thomas C Schuler, Diana S DeWolfe
BACKGROUND CONTEXT: Percutaneous intradiscal therapies are gaining popularity as a regenerative treatment option for spinal disc degeneration. The risks, benefits, and possible complications associated with such procedures have been poorly defined. As these procedures are performed with increasing frequency, the likelihood that clinicians will be faced with significant complications also increases. PURPOSE: The purpose of this study is to describe a significant complication of a percutaneous intradiscal bone marrow and adipose tissue transplantation for symptomatic lumbar disc degeneration...
November 2012: Spine Journal: Official Journal of the North American Spine Society
Peter Ghosh, Robert Moore, Barrie Vernon-Roberts, Tony Goldschlager, Diane Pascoe, Andrew Zannettino, Stan Gronthos, Silviu Itescu
OBJECT: Chronic low-back pain of discal origin is linked strongly to disc degeneration. Current nonsurgical treatments are palliative and fail to restore the disc extracellular matrix. In this study the authors examined the capacity of ovine mesenchymal precursor cells (MPCs) to restore the extracellular matrix of degenerate discs in an ovine model. METHODS: Three adjacent lumbar discs of 24 adult male sheep were injected intradiscally with chondroitinase-ABC (cABC) to initiate disc degeneration...
May 2012: Journal of Neurosurgery. Spine
Michael Bendtsen, Cody Eric Bünger, Xuenong Zou, Casper Foldager, Hans Stødkilde Jørgensen
STUDY DESIGN: Experimental, controlled, randomized, and paired study. OBJECTIVE: To evaluate regenerative effect of stem cell therapy on the vertebral endplate and introduce dynamic contrast-enhanced magnetic resonance imaging (MRI) as a tool in the investigation of endplate function. SUMMARY OF BACKGROUND DATA: The vertebral endplate plays a crucial role in nutritional supply to the intervertebral disc. Estimation of endplate function is an important parameter in future biologic therapy of intervertebral disc degeneration (IDD)...
March 15, 2011: Spine
Shishu Huang, Vivian Tam, Kenneth M C Cheung, Dan Long, Minmin Lv, Ting Wang, Guangqian Zhou
Degeneration of the intervertebral disc is an age-related progressive process considered to be the major cause of a series of spine disorders, such as low-back pain that affects the majority of adult population and causes a huge loss of time from work and medical expenses. Numerous regenerative approaches are being developed with the aim to halt or reverse degeneration, including intradiscal administration of nucleus pulposus cells and mesenchymal stem cells and anabolic growth factors. Each of the currently proposed approaches, however, has exhibited certain limitations or shortcomings, largely due to our limited understanding on the cell biology, turnover mechanisms of the intervertebral disc as well as the etiology of disc degeneration...
December 2011: Current Stem Cell Research & Therapy
Takashi Miyamoto, Takeshi Muneta, Takashi Tabuchi, Kenji Matsumoto, Hirohisa Saito, Kunikazu Tsuji, Ichiro Sekiya
INTRODUCTION: Synovial mesenchymal stem cells (MSCs) have high proliferative and chondrogenic potentials, and MSCs transplanted into the articular cartilage defect produce abundant extracellular matrix. Because of similarities between the articular cartilage and the intervertebral disc cartilage, synovial MSCs are a potential cell source for disc regeneration. Here, we examined the effect of intradiscal transplantation of synovial MSCs after aspiration of nucleus pulposus in rabbits. METHODS: The nucleus pulposus tissues of rabbit's intervertebral discs were aspirated to induce disc degeneration, and allogenic synovial MSCs were transplanted...
2010: Arthritis Research & Therapy
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