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https://www.readbyqxmd.com/read/29778881/polymerase-chain-reaction-pcr-based-methods-promising-molecular-tools-in-dentistry
#1
REVIEW
Shahriar Shahi, Sepideh Zununi Vahed, Nazanin Fathi, Simin Sharifi
Polymerase chain reaction (PCR) has become a popular diagnosis and research technique in dentistry. Several studies show that its high sensitivity and specificity allow it as a precise, efficient, and rapid method for detection, identification, and quantification of microorganism. Several genetic polymorphisms can be determined along with detection of immune and inflammatory markers, therefore providing the more accurate perception into the mechanisms underlying the dental and periodontal disease. This review paper discusses the application of PCR as a diagnostic technique in periodontology, endodontic infections, implant-related, and peri-implantitis infection, immune and inflammatory markers identification and genetic polymorphism as well as application of next generation sequencing and gene microarray technology in dentistry mentioned...
May 17, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29770323/dental-mesenchymal-stem-cell-based-translational-regenerative-dentistry-from-artificial-to-biological-replacement
#2
REVIEW
Mona K Marei, Rania M El Backly
Dentistry is a continuously changing field that has witnessed much advancement in the past century. Prosthodontics is that branch of dentistry that deals with replacing missing teeth using either fixed or removable appliances in an attempt to simulate natural tooth function. Although such "replacement therapies" appear to be easy and economic they fall short of ever coming close to their natural counterparts. Complications that arise often lead to failures and frequent repairs of such devices which seldom allow true physiological function of dental and oral-maxillofacial tissues...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29770239/comparison-of-marginal-bone-loss-between-internal-and-external-connection-dental-implants-in-posterior-areas-without-periodontal-or-peri-implant-disease
#3
Dae-Hyun Kim, Hyun Ju Kim, Sungtae Kim, Ki-Tae Koo, Tae-Il Kim, Yang-Jo Seol, Yong-Moo Lee, Young Ku, In-Chul Rhyu
Purpose: The purpose of this retrospective study with 4-12 years of follow-up was to compare the marginal bone loss (MBL) between external-connection (EC) and internal-connection (IC) dental implants in posterior areas without periodontal or peri-implant disease on the adjacent teeth or implants. Additional factors influencing MBL were also evaluated. Methods: This retrospective study was performed using dental records and radiographic data obtained from patients who had undergone dental implant treatment in the posterior area from March 2006 to March 2007...
April 2018: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29763494/expression-levels-of-semaphorins-3a-3b-4a-and-4d-on-human-peri-implantitis
#4
Marta Ferreira Bastos, Leonardo de Franco, Andressa Cristina Garcia Tebar, Gabriela Giro, Jamil Awad Shibli
PURPOSE: To evaluate the gene expression levels of semaphorins 3A, 3B, 4A, and 4D in both healthy and diseased implants. MATERIALS AND METHODS: Subjects with peri-implantitis presented clinical attachment loss, probing depth ≥ 5 mm, bleeding on probing and/or suppuration, and radiographic bone loss > 4 mm. Peri-implant tissue biopsy specimens were sampled for analysis of the mRNA expression levels for semaphorins 3A, 3B, 4A, and 4D. A real-time polymerase chain reaction was performed, and the gene expression levels of semaphorins in relation to the housekeeping gene were analyzed by using the nonparametric Mann-Whitney test (P < ...
May 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29761866/occurrence-of-peri-implant-diseases-and-risk-indicators-at-the-patient-and-implant-levels-a-multi-level-cross-sectional-study
#5
Suzana P Pimentel, Roberto Shiota, Fabiano R Cirano, Renato C V Casarin, Vanessa G A Pecorari, Marcio Z Casati, Alex N Haas, Fernanda V Ribeiro
BACKGROUND: High prevalence rates of peri-implant diseases have been reported; however, the lack of standardization of definition criteria has lead to variations in the observed estimates. In addition, scarce data is available concerning patient and implant related factors associated to peri-implantitis. The aim of this study was to determine the prevalence of peri-implant diseases and their risk indicators at the patient and implant levels. METHODS: 147 patients with 490 dental implants were included...
May 15, 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29758001/an-antimicrobial-peptide-loaded-gelatin-chitosan-nanofibrous-membrane-fabricated-by-sequential-layer-by-layer-electrospinning-and-electrospraying-techniques
#6
Yuzhu He, Yahui Jin, Xiumei Wang, Shenglian Yao, Yuanyuan Li, Qiong Wu, Guowu Ma, Fuzhai Cui, Huiying Liu
Guided bone regeneration (GBR) technique is widely used in the treatment of bone defects caused by peri-implantitis, periodontal disease, etc. However, the GBR membranes commonly used in clinical treatments currently have no antibacterial activity. Therefore, in this study, sequential layer-by-layer electrospinning and electrospraying techniques were utilized to prepare a gelatin (Gln) and chitosan (CS) composite GBR membrane containing hydroxyapatite nanoparticles (nHAp) and antimicrobial peptide (Pac-525)-loaded PLGA microspheres (AMP@PLGA-MS), which was supposed to have osteogenic and antibacterial activities...
May 14, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29756133/biology-of-teeth-and-implants-host-factors-pathology-regeneration-and-the-role-of-stem-cells
#7
F-Michael Eggert, Liran Levin
In chronic periodontitis and peri-implantitis, cells of the innate and adaptive immune systems are involved directly in the lesions within the tissues of the patient. Absence of a periodontal ligament around implants does not prevent a biologic process similar to that of periodontitis from affecting osseointegration. Our first focus is on factors in the biology of individuals that are responsible for the susceptibility of such individuals to chronic periodontitis and to peri-implantitis. Genetic factors are of significant importance in susceptibility to these diseases...
2018: Quintessence International
https://www.readbyqxmd.com/read/29753881/confocal-fluorescence-imaging-to-evaluate-the-effect-of-antimicrobial-photodynamic-therapy-depth-on-p-gingivalis-and-t-denticola-biofilms
#8
Stephen Rogers, Kiyonobu Honma, Thomas S Mang
BACKGROUND: Porphyromonas gingivalis and Treponema denticola are both principally implicated in the incidence of both periodontal disease and peri-implantitis. Recent studies have demonstrated that these bacteria exhibit symbiotic growth in vitro and a synergistic virulence in co-infection of animal models. Found at varying depths throughout the biofilm, these bacteria present a significant challenge to traditional antimicrobial treatment modalities. Antimicrobial photodynamic therapy ( aPDT ) has yielded high success against bacterial biofilms, namely those found in the oral cavity...
May 10, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29744214/comparison-of-biofilm-formation-and-migration-of-streptococcus-mutans-on-tooth-roots-and-titanium-miniscrews
#9
Kittipong Laosuwan, Don Jeevanie Epasinghe, Zhaoming Wu, Wai Keung Leung, David William Green, Han Sung Jung
Periodontitis and peri-implantitis are inflammatory diseases caused by periodontal pathogenic bacteria leading to destruction of supporting periodontal/peri-implant tissue. However, the progression of inflammatory process of these two diseases is different. The bacterial biofilm is the source of bacteria during the inflammatory process. As the bacteria migrate down the surface of tooth or titanium implant, the inflammation spreads along with it. Streptococcus mutans has an important role in oral bacterial biofilm formation in early stage biofilm before the microbiota shift to late stage and become more virulent...
April 2018: Clinical and Experimental Dental Research
https://www.readbyqxmd.com/read/29709064/effects-of-moderate-intensity-static-magnetic-fields-on-osteoclastic-differentiation-in-mouse-bone-marrow-cells
#10
Eun-Cheol Kim, Jaesuh Park, Gunwoo Noh, Su-Jung Park, Kwantae Noh, Il-Keun Kwon, Su-Jin Ahn
Although we recently demonstrated that static magnetic fields (SMFs) of 3, 15, and 50 mT stimulate osteoblastic differentiation, the effects of SMFs on osteoclastogenesis are still poorly understood. This study focused on the suppressive effects of SMFs on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis and bone resorption. Direct SMFs inhibit RANKL-induced multinucleated osteoclast formation, tartrate-resistant acid phosphatase activity, and bone resorption in mouse bone marrow-derived macrophage cells...
April 30, 2018: Bioelectromagnetics
https://www.readbyqxmd.com/read/29706393/implant-success-and-safety-of-left-atrial-appendage-occlusion-in-end-stage-renal-disease-patients-peri-procedural-outcomes-from-an-italian-dialysis-population
#11
Simonetta Genovesi, Giorgio Slaviero, Luca Porcu, Gavino Casu, Silvio Bertoli, Antonio Sagone, Federico Pieruzzi, Giovanni Rovaris, Monique Buskermolen, Paolo Danna, Alberto Montoli, Jacopo Oreglia, Gina Contaldo, Patrizio Mazzone
AIMS: To estimate the safety and the efficacy of the off label left atrial appendage (LAA) occlusion in chronic dialysis patients with atrial fibrillation (AF). In this preliminary paper, we report the design of the study and the data on peri-procedural complications. METHODS: This is a prospective cohort study. Primary endpoints are i) incidence of peri-procedural complications, ii) cumulative incidence of two-year thromboembolic events iii) cumulative incidence of two-year bleedings iiii) mortality at two years...
July 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29706210/influence-of-antibiotic-prophylaxis-on-the-stability-of-orthodontic-microimplants-a-pilot-randomized-controlled-trial
#12
RANDOMIZED CONTROLLED TRIAL
Jan Łyczek, Beata Kawala, Joanna Antoszewska-Smith
INTRODUCTION: The aims of this 2-arm parallel pilot randomized controlled trial were to investigate the influence of antibiotic prophylaxis on the stability of orthodontic microimplants and to evaluate the efficacy of systemic inflammatory marker measurements in detecting infections in tissues surrounding microscrews. METHODS: Orthodontic patients requiring en-masse distalization in the maxilla received antibiotics or a placebo before microimplant placement. Eligibility criteria included 13 years of age, and good general and oral health...
May 2018: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/29696649/the-efficacy-of-laser-therapy-used-alone-or-as-an-adjunct-to-non-surgical-and-surgical-treatment-of-periodontitis-and-peri-implant-diseases
#13
Kirk W Noraian, Charles M Cobb
Twenty-five years of research focused on various aspects of laser-mediated periodontal therapy have yielded a significant amount of conflicting evidence and a considerable amount of controversy. Despite the inconsistencies and the polemics, the use of lasers, either as a monotherapy or adjunctive to traditional therapy, is still promoted by some expert clinicians, key opinion leaders, and commercial entities. This article is protected by copyright. All rights reserved.
April 25, 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29693260/american-academy-of-periodontology-best-evidence-consensus-statement-on-the-efficacy-of-laser-therapy-used-alone-or-as-an-adjunct-to-non-surgical-and-surgical-treatment-of-periodontitis-and-peri-implant-diseases
#14
Michael P Mills, Paul S Rosen, Leandro Chambrone, Henry Greenwell, Richard T Kao, Perry R Klokkevold, Bradley S McAllister, Mark A Reynolds, Georgios E Romanos, Hom-Lay Wang
The American Academy of Periodontology (AAP) recently embarked on a Best Evidence Consensus (BEC) model of scientific inquiry to address questions of clinical importance in the treatment of periodontal and peri-implant diseases. For each focused question addressed below, there is a critical mass of evidence. However, standing alone, that evidence is, in the judgment of the Expert Panel convened by the AAP, insufficient to support broad conclusions and/or clinical practice guidelines. This article is protected by copyright...
April 25, 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29683503/diagnostic-accuracy-of-clinical-parameters-to-monitor-peri-implant-conditions-a-matched-case-control-study
#15
Alberto Monje, Jordi Caballé-Serrano, Jose Nart, David Peñarrocha, Hom-Lay Wang, Mia Rakic
BACKGROUND: The aim of this case-control study was to estimate the diagnostic accuracy of the standard clinical parameters in diagnosing healthy peri-implant tissues, peri-implant mucositis, and peri-implantitis. METHODS: A case-control study was designed to compare the clinical parameters used in the diagnosis of peri-implant diseases such as: probingdepth (PD), bleeding on probing (BOP), mucosal redness (MR), suppuration (SUP), and plaque index (PI). Furthermore, the influence of patient- (sex, age) and implant-related variables (implant neck configuration, time in function after loading) were evaluated to investigate the association with the clinical findings...
April 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29683501/the-evaluation-of-peri-implant-sulcus-fluid-osteocalcin-osteopontin-and-osteonectin-levels-in-peri-implant-diseases
#16
Oya Turkoglu Cakal, Candan Efeoglu, Emir Bozkurt
BACKGROUND: Peri-implant mucositis is an inflammation of the soft tissues surrounding an implant. Peri-implantitis refers to a process characterized by peri-implant bone loss along with an inflammation of the soft tissues. Osteocalcin, osteopontin, and osteonectin proteins are related to bone remodeling. The aim of the present study was to investigate peri-implant sulcus fluid (PISF) osteocalcin, osteopontin, and osteonectin levels in peri-implant mucositis and peri-implantitis. METHODS: Fifty-two implants with peri-implantitis, 46 implants with peri-implant mucositis, and 47 control implants were included in the study...
April 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29659049/clinical-characteristics-of-peri-implant-mucositis-and-peri-implantitis
#17
Ausra Ramanauskaite, Kathrin Becker, Frank Schwarz
OBJECTIVES: To evaluate and correlate clinical parameters associated with peri-implant diseases based on established case definitions. MATERIALS AND METHODS: A total of 75 patients exhibiting 269 implants (healthy: 77; peri-implant mucositis: 77; peri-implantitis: 115) were included in this observational study. Clinical parameters included bleeding on probing (BOP), probing depths (PDs), and suppuration (Supp). RESULTS: Healthy sites were associated with the absence of BOP, while mean BOP in peri-implant mucositis and peri-implantitis patients amounted to 20...
April 16, 2018: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/29656074/protein-phosphatase-2a-as-a-new-target-for-downregulating-osteoclastogenesis-and-alleviating-titanium-particle-induced-bone-resorption
#18
Liangliang Wang, Xiaobin Guo, Wei Zhou, Yayun Ding, Jiawei Shi, Xiexing Wu, Yu Liu, Yaozeng Xu, Huilin Yang, Dechun Geng
Receptor activator of nuclear factor-кB ligand (RANKL)-induced osteoclastogenesis is believed to play a critical role in osteolytic diseases including peri-prosthetic osteolysis (PPO), the primary reason for implant failure and revision surgery. In this study, we observed that protein phosphatase 2A (PP2A), a major serine-threonine phosphatase, was highly expressed in human periprosthetic interface membranes with aseptic loosening and in a murine osteolysis model induced by titanium particle irritation. PP2A inhibition effectively alleviated titanium particle-induced bone destruction at osteolytic sites...
April 12, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29581951/unusual-malignant-neoplasms-occurring-around-dental-implants-a-report-of-2-cases
#19
Song Hee Oh, Ju Hee Kang, Yu-Kyeong Seo, Sae Rom Lee, Yong-Suk Choi, Eui-Hwan Hwang
Osseointegrated implants are now commonplace in contemporary dentistry. However, a number of complications can occur around dental implants, including peri-implantitis, maxillary sinusitis, osteomyelitis, and neoplasms. There have been several reports of a malignant neoplasm occurring adjacent to a dental implant. In this report, we describe 2 such cases. One case was that of a 75-year-old man with no previous history of malignant disease who developed a solitary plasmacytoma around a dental implant in the left posterior mandible, and the other was that of a 43-year-old man who was diagnosed with squamous cell carcinoma adjacent to a dental implant in the right posterior mandible...
March 2018: Imaging Science in Dentistry
https://www.readbyqxmd.com/read/29576918/nonspanning-total-wrist-arthrodesis-with-a-low-profile-locking-plate
#20
Schneider K Rancy, Eugene T Ek, Sophia Paul, Robert N Hotchkiss, Scott W Wolfe
Purpose  This study aims to compare the outcomes and complications of our technique for nonspanning total wrist arthrodesis using a locking plate with the standard carpometacarpal spanning technique. Methods  A retrospective review of charts was performed to identify patients who underwent total wrist arthrodesis by the senior author (S.W.W.). We compared the outcomes of 15 cases of nonspanning wrist fusion with a 2.4/2.7 mm locking T plate to 11 cases of spanning wrist fusion with a 2.7/3.5 mm locking compression plate...
April 2018: Journal of Wrist Surgery
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